Labconco 794001010, 794001015, 794001030, 794001040, 794001050 Instruction Manual

Original instructions

FreeZone® Triad™ Freeze Dryers

Models

7940010** Series

Original instructions

Warranty

Labconco Corporation provides a warranty to the original buyer for the repair or replacement of parts and
reasonable labor as a result of normal and proper use of the equipment with compatible chemicals. Broken
glassware and maintenance items, such as filters, gaskets, light bulbs, finishes and lubrication are not
warranted. Excluded from warranty are products with improper installation, erratic electrical or utility supply,
unauthorized repair and products used with incompatible chemicals.

The warranty for FreeZone® Freeze Dry Systems will expire one year from date of installation or two years
from date of shipment from Labconco, whichever is sooner. Warranty is non-transferable and only applies to
the owner (organization) of record.

Buyer is exclusively responsible for the set-up, installation, verification, decontamination or calibration of
equipment. This limited warranty covers parts and labor, but not transportation and insurance charges. If the
failure is determined to be covered under this warranty, the dealer or Labconco Corporation will authorize
repair or replacement of all defective parts to restore the unit to operation. Repairs may be completed by 3rd
party service agents approved by Labconco Corporation. Labconco Corporation reserves the rights to limit this

warranty based on a service agent’s travel, working hours, the site’s entry restrictions and unobstructed access

to serviceable components of the product.
Under no circumstances shall Labconco Corporation be liable for indirect, consequential, or special damages

of any kind. This warranty is exclusive and in lieu of all other warranties whether oral, or implied.

Copyright © 2017 Labconco Corporation. The information contained in this manual and the accompanying
products are copyrighted and all rights reserved by Labconco Corporation. Labconco Corporation reserves
the right to make periodic design changes without obligation to notify any person or entity of such change.

Returned or Damaged Goods

Do not return goods without the prior authorization from Labconco. Unauthorized returns will not be
accepted. If your shipment was damaged in transit, you must file a claim directly with the freight carrier.
Labconco Corporation and its dealers are not responsible for shipping damages.

The United States Interstate Commerce Commission rules require that claims be filed with the delivery
carrier within fifteen (15) days of delivery.

Limitation of Liability

The disposal and/or emission of substances used in connection with this equipment may be governed by
various federal, state, or local regulations. All users of this equipment are required to become familiar with
any regulations that apply in the user’s area concerning the dumping of waste materials in or upon water,
land, or air and to comply with such regulations. Labconco Corporation is held harmless with respect to
user’s compliance with such regulations.

Contacting Labconco Corporation

If you have questions that are not addressed in this manual, or if you need technical assistance, contact
Labconco’s Customer Service Department or Labconco’s Product Service Department at 1-800-821-5525
or 1-816-333-8811, between the hours of 7:30 a.m. and 5:30 p.m., Central Standard Time.

Part #7343300, Rev. A

ECO L423

Original instructions

TABLE OF CONTENTS

CHAPTER 1: INTRODUCTION 1
Intended Use 1
Freeze Dry Process 1
Freeze Dry Rates 2
Freeze Dry Capacity 3
Samples Containing Volatile Substances 4
About This Manual 4
Typographical Conventions 5

CHAPTER 2: PREREQUISITES 6

Electrical Requirements 6
Location Requirements 7
Vacuum Pump Requirements 7

CHAPTER 3: GETTING STARTED 8

Unpacking Your Freeze Dryer 8
Freeze Dryer Components 9

Setting Up Your Freeze Dryer 9
Component Identification 10
Vacuum Pump Connection 11
Electrical Connection 12
Chemical Resistance of Freeze Dryer Components 12

Solvent Safety Precautions 14

CHAPTER 4: LYO-WORKS™ OPERATING SYSTEM 15

Control Panel 16
Initial Power Up 16
Touch Screen Operation 17

Home screen 17

Main Menu Icons 17

Moving between Menu Screens 17
Function Indicator Bar 17
System Parameters Display 18
Operation Buttons (Program, Manual, Vacuum, Defrost) 19

Programs screen 20

Data Logging screen 22

Settings screen 23

Original instructions

Language 23
Time & Date 23
Units 24
Security 24
Alerts 25
Configure Email Settings 26
Maintenance 27

Vacuum Pump Settings 27

Refrigeration System Settings 27
Freeze Dryer Line Voltage Offset 27
Vacuum Pump Protection 27

Calibration 28
Display 29

Network 30
Sensors screen 30
End Point Detection screen 30
Help screen 32
Diagnostics 32
User’s Manual 32
About 32

CHAPTER 5: USING YOUR FREEZE DRYER 33

Operation Checklist 33
Vacuum Pump Ballast Setting 33
Sample Freezing 33
Shelf Loading 34
Setting the Operating Vacuum Level 34
Freeze Drying Inside the Chamber 36
Manual Mode with Pre-Frozen Samples 36
Manual Mode with Unfrozen Samples 37
Program Mode with Pre-Frozen Samples 37
Program Mode with Unfrozen Samples 38
Stopping the Freeze Dry Cycle 38
Freeze Drying Using Sample Valves (In-Flask Mode) 39
Stoppering Vials 40
Vacuum Break/Backfilling 40
Defrosting 41
Alerts 41
Copy (Export) a Data Log File 43
Download a Data Log File via Ethernet 43
Copy a Program 44

CHAPTER 6: MAINTAINING YOUR FREEZE DRYER 45

Service Safety Precautions 45
Routine Maintenance Schedule 45

Original instructions

CHAPTER 7: TROUBLESHOOTING 47

Vacuum Pump 47
Gaskets, Tubing, Connections, Sample Valves 48
System Components & Collector Chamber Isolation 50
Refrigeration Module Operation 52

APPENDIX A: FREEZE DRYER COMPONENTS 53

APPENDIX B: FREEZE DRYER DIMENSIONS 55

APPENDIX C: FREEZE DRYER SPECIFICATIONS 56

Electrical Specifications 56
Environmental Conditions 57

APPENDIX D: FREEZE DRYER ACCESSORIES 58

1

Original instructions

CHAPTER 1

INTRODUCTION

Congratulations on your purchase of a Labconco FreeZone® Triad™ Freeze Dry
System, which is designed for laboratory lyophilization procedures. The
refrigerant used in the refrigeration system is HCFC-free so it will not endanger
the environment. The unit is easy to install and maintain. Proper care and
maintenance of this product will result in many years of dependable service.

Intended Use

The Labconco Freeze Dryers described in this manual are intended for laboratory
use to facilitate the lyophilization process.

Freeze Dry Process

Freeze drying is an important process in sample preparation and for the preservation
and storage of biologicals, pharmaceuticals and foods. Of the various methods of
dehydration, freeze drying (lyophilization) is especially suited for substances that are
heat sensitive. Other than food processing (e.g., coffee, whole dinners), freeze drying
has been extensively used in the development of pharmaceuticals (e.g., antibiotics) and
preservation of biologicals (e.g., proteins, plasma, viruses and cell lines). The
nondestructive nature of this process has been demonstrated by the retention of
viability in freeze dried viruses and microorganisms.

Freeze drying is a process whereby water or other solvent is removed from frozen
material by converting the frozen water directly into vapor without the
intermediate formation of liquid water. The basis for this sublimation process
involves the absorption of heat by the frozen sample in order to vaporize the ice;
the use of a vacuum pump to enhance the removal of water vapor from the surface
of the sample; the transfer of water vapor to a collector; and the removal of heat by
the collector in order to condense the water vapor. In essence, the freeze dry
process is a balance between the heat absorbed by the sample to vaporize the ice,
and the heat removed from the collector to convert the water vapor into ice.

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Original instructions

SAFE TEMPERATURE AND DRYING TIMES

FOR SELECTED MATERIALS

Material
10mm Thick

Safe
Temperature
°C

Collector
Temperature
°C

Hours
(Approx.)
Milk

-5

-40

10

Urea

-7

-40

10

Blood Plasma

-10 to -25

-40

16

Serum

-25

-40

18

Vaccinia

-30 to -40

-50

22

Influenza Vaccine

-30

-50

24

Human Tissue

-30 to -40

-50

48

Vegetable Tissue

-50

-80

60

Table 1

Freeze Dry Rates

The efficiency of the Freeze Drying process is dependent upon the surface area and
the thickness of the sample, the collector temperature and vacuum obtained, the
eutectic point and solute concentration of the sample. It is important to remember
these factors when trying to obtain efficient utilization of your Freeze Dry system.
A listing of selected materials and their approximate drying times are shown in
Table 1 for your reference.

*Total sample quantities are contingent on various freeze dryer capacities.

Up to the point of overloading the system, the greater the surface area of the
sample, the faster the rate of freeze drying. By contrast, for a given surface area,
the thicker the sample the slower the rate of freeze drying. This is based on the
fact that the heat of sublimation is usually absorbed on one side of the frozen
sample and must travel through the frozen layer to vaporize water at the other
surface. In addition, as the sample is freeze dried, the water vapor must travel
through the layer of dried material. The thicker the sample, the greater the chance
that the dried layer may collapse which would cause an additional decrease in the
rate of freeze drying.

The surface area and thickness of the sample can usually be ignored when each
sample contains only a few milliliters. However, for larger volumes, the samples
should be shell frozen to maximize the surface area and minimize the thickness of
the sample. The volume of the freeze dry flask should be two to three times the
volume of the sample.

In order for lyophilization to occur, ice must be removed from the frozen sample
via sublimation. This is accomplished by the collector and the vacuum pump. The
collector, which should be at least 15 to 20°C colder than the eutectic temperature
(melting temperature) of the sample, traps vapor as ice. Since the vapor pressure
at the collector is lower than that of the sample, the flow of water vapor is from the
sample to the collector. Since this vapor diffusion process occurs very slowly
under normal atmospheric conditions, a good vacuum is essential to maintain an

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Chapter 1: Introduction

3

Original instructions

efficient rate. In many applications, the maintenance of a vacuum of 0.133 mbar
or less is recommended.

The rate of freeze drying is directly proportional to the vapor pressure and the
vapor pressure is dependent upon both eutectic temperature and solute
concentration of the sample. For example, a solution of sodium chloride and water
would freeze dry at a slower rate than pure water. The eutectic temperature of a
sodium chloride solution is about –21°C and at this temperature the vapor pressure
is about 1/16 that of water at 0°C. Although the eutectic temperature is not
dependent upon the concentration of sodium chloride, the vapor pressure of the
water would decrease as the concentration of sodium chloride increased. This is
due to the fact that as the solute concentration increases, less of the surface area of
the frozen sample is occupied by water. In general, most solutions or biological
samples will have a eutectic temperature of –10 to –25°C. However, if the sample
contains a simple sugar such as glucose or if the sample is animal or plant tissue,
the eutectic temperature may be as low as –30° to –50°C.

Freeze Dry Capacity

The volume of a sample that can be freeze dried at one time is related to factors
discussed previously and the size and design of the freeze dry system. With any
given instrument, the capacity is based on the surface area of the sample; the
eutectic temperature and concentration of the sample; and the rate and amount of
heat transferred to the frozen sample. Of these factors, the eutectic temperature is
the most important factor in determining the amount of sample that can be freeze
dried at one time, particularly when flasks are used. This is because as the eutectic
temperature decreases, the vapor pressure decreases but the rate of heat absorption
by the sample does not change. This tends to promote melting of the sample,
which leads to a marked increase in vapor pressure and ultimately overloads the
collector and vacuum pump. Samples that have eutectic temperatures of –20°C or
lower should be placed on the freeze dry system one flask at a time so that the
vacuum in the system may recover before adding another sample to the system. If
the vacuum does not recover, the capacity of the freeze dry system has been
exceeded and the sample should be removed.

If there is a problem with a particular type of sample melting when placed on the
freeze dry system, dilution of the sample with more water or providing some
insulation around the flask (when utilizing In-Flask mode) to decrease the rate of
heat absorption by the sample may help.

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Chapter 1: Introduction

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Original instructions

Samples Containing Volatile Substances

In certain cases the solvent in a sample to be freeze dried may contain volatile
components such as acetonitrile, methanol, acetic acid, formic acid or pyridine. In
addition to these substances having an effect on the eutectic temperature, they may
increase the vapor pressure at the surface of the sample. Also, compared to water,
they will require the absorption of less heat for sublimation to occur. Hence,
samples that contain volatile substances will have a greater tendency to melt,
particularly when placed in flasks or exposed to room temperature. If a sample
containing a volatile substance tends to melt when placed on a freeze dry system,
dilution of the sample with more water will help keep the sample frozen. For
example, a 0.2M solution of acetic acid is much easier to freeze dry than a 0.5M
solution.

About This Manual

This manual is designed to help you learn how to install, use, and maintain your
Freeze Dryer. Instructions for performing routine maintenance and making minor
modifications to your Freeze Dryer are also included.

Chapter 1: Introduction provides a brief overview of the Freeze Dryer, explains
the organization of the manual, and defines the typographical conventions used in
the manual.

Chapter 2: Prerequisites explains what you need to do to prepare your site before
you install your Freeze Dryer. Electrical requirements are discussed.

Chapter 3: Getting Started contains the information you need to properly unpack,
inspect and install your Freeze Dryer.

Chapter 4: Lyo-Works Operating System describes the display screens and user
interface software that controls the Freeze Dryer.

Chapter 5: Using Your Freeze Dryer discusses the basic operation of your Freeze
Dryer. Information on how to attach samples and run the Freeze Dryer is included.

Chapter 6: Maintaining Your Freeze Dryer explains how to perform routine
maintenance on your Freeze Dryer.

Chapter 7: Troubleshooting contains a table of problems you may encounter while
using your Freeze Dryer, including the probable causes of the problems, and
suggested corrective actions.

Appendix A: Freeze Dryer Components contains labeled diagrams of the
components of the Freeze Dryer.

Appendix B: Freeze Dryer Dimensions contains diagrams showing the dimensions
for the Freeze Dryer.

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Chapter 1: Introduction

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Original instructions



!

Appendix C: Freeze Dryer Specifications contains product specifications.

Appendix D: Freeze Dryer Accessories see www.labconco.com for a complete list

of Freeze Dry accessories.

Typographical Conventions

Recognizing the following typographical conventions will help you understand and
use this manual:

 Book, chapter, and section titles are shown in italic type (e.g., Chapter 3:

Getting Started).

 Steps required to perform a task are presented in a numbered format.
 Comments located in the margins provide suggestions, reminders, and

references.

 Critical information is presented in boldface type in paragraphs that are

preceded by the exclamation icon. Failure to comply with the information
following an exclamation icon may result in injury to the user or permanent
damage to your Freeze Dryer.

 Important information is presented in capitalized type in paragraphs that are

preceded by the pointer icon. It is imperative that the information contained in
these paragraphs be thoroughly read and understood by the user.

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6

Original instructions

CHAPTER 2

PREREQUISITES

Before you install your Freeze Dryer, you need to prepare your site for installation.
Carefully examine the location where you intend to install your Freeze Dryer. You
must be certain that the area is level and of solid construction. An electrical source
must be located near the installation site.

Carefully read this chapter to learn:

 Electrical supply requirements.
 Vacuum pump requirements.

Refer to Appendix C: Freeze Dryer Specifications for complete Freeze Dryer
electrical and environmental conditions, specifications and requirements.

Electrical Requirements

 230V models

o 20 Amp dedicated single phase circuit.
o Properly grounded receptacle that matches the plug supplied with

the unit, (230V models are supplied with one of the following
plugs):

US EU China UK India

(NEMA 6-15P)

o If the plug does not match the available receptacle, remove the plug

and replace it with an approved plug of the suitable style.

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Chapter 2: Prerequisites

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Original instructions



!

!

Do not use any detachable power cord that is not adequately
rated for the unit.

Frequency must agree with the serial tag rating.
Improper frequency will damage the compressor.

ADDITIONAL APPLIANCES ON THE SAME CIRCUIT MAY
CAUSE THE CURRENT TO EXCEED THE RATING OF THE
CIRCUIT BREAKER OR FUSE.

Location Requirements

The Freeze Dryer should be located in an area that provides an unobstructed flow
of air around the cabinet. This air cools the refrigeration system. The refrigeration
system draws air through the rear panel and exhausts it through the sides. A
minimum of 3" must be allowed between the back and both sides of the Freeze
Dryer and adjacent wall surfaces. Restriction of airflow during operation could
adversely affect performance. The Triad Freeze Dryer must be placed on a work
surface or table that will support over 400 lbs.

Refer to Appendix B: Freeze Dryer Dimensions for dimensional drawings.

Vacuum Pump Requirements

A vacuum pump must be provided by the user. A vacuum pump with a
displacement of 98 liters per minute and 0.007 mbar ultimate pressure is adequate
for most samples. The inlet fitting on the vacuum pump must be suitable for 3/4"
ID vacuum hose, which is provided. It is recommended that the vacuum pump is
equipped with an exhaust filter to minimize oil mist exhausting from the vacuum
pump. The operating vacuum level may be set on the Freeze Dryer. The higher
the vacuum set point, the more likely it is that oil mist will be exhausted.

Vacuum pumps should be equipped with a reverse IEC plug (included with 230V
pumps purchased from Labconco). This will allow the vacuum pump to be
plugged into the receptacle on the back panel of the freeze dryer.

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8

Original instructions

!

The United
States
Interstate
Commerce
Commission
rules require
that claims be
filed with the
delivery carrier
within fifteen
(15) days of
delivery.



CHAPTER 3

GETTING STARTED

Now that the site for your Freeze Dryer is properly prepared, you are ready to
unpack, inspect, install and test your Freeze Dryer. Read this chapter to learn how
to:

 Unpack and move your Freeze Dryer.
 Set up your Freeze Dryer.
 Connect the electrical supply source to your Freeze Dryer.
 Properly exhaust your Freeze Dryer.
 Safely use solvents with your Freeze Dryer.

The Triad Freeze Dryers weigh over 400 lbs (181 Kg). The
carton allows for lifting with a mechanical lift truck or hand
truck. If you must lift the Freeze Dryer manually, use at least
two (4) persons and follow safe lifting guidelines.

Unpacking Your Freeze Dryer

Carefully unpack your Freeze Dryer and inspect it for damage that may have
occurred in transit. If your Freeze Dryer is damaged, notify the delivery carrier
immediately and retain the entire shipment intact for inspection by the carrier.

DO NOT RETURN GOODS WITHOUT THE PRIOR
AUTHORIZATION OF LABCONCO. UNAUTHORIZED
RETURNS WILL NOT BE ACCEPTED.

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Chapter 3: Getting Started

9

Original instructions

Qty.

Part #

Description

Comments

1

1089005

User’s Manual

USB Flash Drive

1

Various

Power Cord

See parts list (Appendix A)

1

7373450

Vacuum Hose

2

1488800

Hose Clamp





IF YOUR FREEZE DRYER WAS DAMAGED IN TRANSIT,
YOU MUST FILE A CLAIM DIRECTLY WITH THE FREIGHT
CARRIER. LABCONCO CORPORATION AND ITS DEALERS
ARE NOT RESPONSIBLE FOR SHIPPING DAMAGE.

DO NOT DISCARD THE CARTON OR PACKING MATERIAL
FOR YOUR FREEZE DRYER UNTIL YOU HAVE CHECKED
ALL OF THE COMPONENTS AND INSTALLED AND TESTED
THE FREEZE DRYER.

Freeze Dryer Components

Verify that the components listed below are present and undamaged.

If you did not receive one or more of the components listed for your Freeze Dryer,
or if any of the components are damaged, contact Labconco Corporation
immediately for further instructions.

Setting Up Your Freeze Dryer

After you verify receipt of the proper components, move your Freeze Dryer to the
location where you want to install it. Then, follow the steps listed below.

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Chapter 3: Getting Started

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Original instructions

Sample Valves

USB Port

Touch Screen

Stoppering Control

Vacuum Release

Backfill Port

On/Standby Button

LED Indicator

Ethernet Port

End Point Detection

Vacuum Pump Outlet

Power Inlet

Vacuum Connection

Circuit Breaker Reset

Component Identification

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Chapter 3: Getting Started

11

Original instructions

HOSE
CLAMP

VACUUM HOSE
3/4" ID

VACUUM
PUMP

!

Hose Barb

Clamp

Inlet

Vacuum Pump Connection

A vacuum pump (as described in Chapter 2: Prerequisites) is required to operate
your Freeze Dryer properly. The Freeze Dryer is equipped with a 3/4" ID vacuum
hose for connecting the collector chamber to the vacuum pump.

Make certain that the oil in the vacuum pump is at the proper
level.

1. Place the vacuum pump near the Triad Freeze

Dryer.

2. Remove protective caps from inlet and outlet

ports.

3. Some vacuum pumps have inlet fittings that clamp

to the pump. Place the centering ring with filter
screen on the inlet fitting.

4. Place the hose barb fitting on top of the centering

ring and secure with the clamp.

5. CHECK THE VACUUM PUMP OIL LEVEL.

Fill the pump with oil so the level is between
the lines on the sight gauge on the end of the
pump housing.

6. Attach one end of the vacuum hose to the port

on the back of the Freeze Dryer and the other
end to the inlet port of the vacuum pump with
the hose clamps provided. If necessary, cut
the hose to proper length to allow for gentle
bends without kinks.

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Original instructions



7. VERIFY THE VACUUM PUMP VOLTAGE SETTING AGREES WITH

THE FREEZE DRYER VOLTAGE SHOWN ON THE SERIAL
NUMBER LABEL. Reconfigure if necessary (see vacuum pump
instructions).

8. Connect the vacuum pump power cord to the receptacle labeled “vacuum

pump” on the back of the Freeze Dryer. Models rated for use on 230V
have a reverse IEC receptacle for attaching the vacuum pump power cord.

9. If the vacuum pump has an ON/OFF switch, turn the switch ON. The

vacuum pump will be controlled by the Freeze Dryer.

IT IS HIGHLY RECOMMENDED THAT AN OIL MIST
EXHAUST FILTER BE INSTALLED ON ROTORY VANE
VACUUM PUMPS TO MINIMIZE OIL MIST EXITING THE
PUMP. AN OIL RETURN KIT CAN ALSO BE USED IN
CONJUNCTION WITH THE EXHAUST FILTER TO RETURN
TRAPPED OIL BACK TO THE VACUUM PUMP.

Electrical Connection

Make sure that the outlet that you intend to use meets the voltage and amperage
requirements listed on the serial tag of the Freeze Dryer. Plug the power cord into
the receptacle on the back of the Freeze Dryer and plug the other end into a
suitable power outlet. It will take approximately 90 seconds for the operating
system to fully load.

Chemical Resistance of Freeze Dryer

Components

The FreeZone Freeze Dry System is designed to be chemically resistant to most
compounds that are commonly used in freeze drying processes. However, by
necessity, the freeze dryer is comprised of a number of different materials, some of
which may be attacked and degraded by certain chemicals. The degree of
degradation is dependent on the concentration and exposure duration. Some of the
major components of the Freeze Dryer that are susceptible to degradation are as
follows:

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Original instructions

Acids

Buffers Solvents

Component

Material

Acetic Acid 20% Formic Acid Trifluoroacetic Acid (TFA)

Calcium Chloride Sodium Phosphate

Acetone Acetonittirle Carbon Tetrochloride Cyclohexane Dioxane Methyl t

-Butyl Ether (BTBE)

Pyridine

Valve Stem

Acetal

C D D D D

Collector
Lid

Acrylic

D D D D

Hoses,
Gaskets &
Valve
Bodies

Neoprene

C D D C C D D D C

D

Flask Top

Silicon
Rubber

C D D D D D C

D

Chamber &
Fittings

Stainless
Steel

C

C – Moderate degradation; Limited use
D – Severe degradation; infrequent use recommended; immediate thorough
cleaning required.

 Most common compounds used in freeze drying processes, if allowed to enter

the vacuum pump, will degrade the oil and cause damage to the vacuum pump.

 Sugars and proteins typically will have minimal negative effect on any of the

materials of construction.

When using compounds in the Freeze Dryer that are hostile to the materials of
construction, it is imperative the equipment is thoroughly cleaned after use.

 Rubber and plastic components that have been exposed to damaging

compounds should be removed and flushed with water.

 The oil in the vacuum pump should be checked often. It must be changed if it

is cloudy, shows particles or is discolored. The useful life of vacuum pump oil
can be extended if the vacuum pump is operated for an extended period of time
after a freeze dry run. This allows contaminants to be purged from the hot oil.
This must be done with the inlet to the pump blocked off to prevent air from
free flowing through the pump. If the pump is operated at an elevated vacuum
level (> 10mbar), oil may be expelled from the pump and damage could occur.

Another way to extend the life of the vacuum pump is to install an optional
secondary trap in the line between the Freeze Dryer and the vacuum pump.
Contact Labconco for ordering information.

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Chapter 3: Getting Started

14

Original instructions

!

With prudent maintenance the Freeze Dryer will provide years of service.
Warranty on the affected parts will be voided if maintenance has been obviously
neglected. If you have questions about using specific compounds in the Freeze
Dryer, contact Labconco Technical Service at 1-800-821-5525 or 816-333-8811 or
e-mail: [e-mail].

Solvent Safety Precautions

Solvents used in the Freeze Dryer may be flammable or
hazardous to your health. Use extreme caution and keep
sources of ignition away from the solvents. When using
flammable or hazardous solvents, the vacuum pump must be
vented to or operated inside a fume hood.

Hazardous materials such as strong acids or bases, radioactive
substances and volatile organics must be handled carefully and
promptly cleaned up if spilled. If a sample is spilled in the
collector chamber it must immediately be cleaned up.

WARNING: The disposal of substances used in connection with
this equipment may be governed by various Federal, State or
local regulations. All users of this equipment are urged to

become familiar with any regulations that apply in the user’s

area concerning the dumping of waste materials in or upon
water, land or air and to comply with such regulations.

Product Service 816-333-8811 or 1-800-522-7658

15

Original instructions

!

CHAPTER 4

LYO-WORKS™ OPERATING SYSTEM

After your Freeze Dryer has been installed as detailed in Chapter 3: Getting
Started, read this chapter to learn how to:

 Operate the controls.
 Understand the display.

Do not use the Freeze Dryer in a manner not specified by the
manufacturer (refer to Appendix C: Freeze Dryer
Specifications). The electrical protection properties of the
Freeze Dryer may be impaired if the Freeze Dryer is used
inappropriately.

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Chapter 4: Lyo-Works Operating System

16

Original instructions

2 3 1

Control Panel

The control panel for the Freeze Dryer is shown below with a description of its
functions.

1. Touch Screen Display – Capacitive touch screen displays system operating

parameters, set-up parameters and alarm messages. All user interface will take
place via the touch screen.

2. On / Standby Button – If the display is illuminated pressing this button will

put the display into low power mode (display backlight will be turned off). If
the display is in low power mode, pressing this button will illuminate the
display (Note: the display can also be brought out of low power mode by
touching any location on the screen).

3. On / Standby LED Indicator – Blue LED indicator light to designate the

current status of the system.

o 1 flash per second = Initial power up (loading operating system)
o Slow pulse = Touch screen in low power mode (screen timeout)
o ON = Unit is plugged in and touch screen is in active mode
o Off = Unit is unplugged

Initial Power Up

When the Freeze Dryer is initially plugged into a power source, the On / Standby
LED will begin flashing (1 flash per second). The display will remain OFF for the
first 10 to 15 seconds, then the FreeZone animated graphics will appear. It will
take approximately 90 seconds for the operating system to fully load.

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Indicator Bar

Time / Date

Operation
Buttons

Menu

Line 2

Line 1

Line 3

Line 4

Line 5

Touch Screen Operation

Home Screen

After initial power up, the Home screen will be displayed. Most of the general
operation and system monitoring can be done from the Home screen.

Main Menu Icons

Menu icons are displayed across the top of the screen. Their descriptions from left
to right are as follows:

1. Home

2. Programs

3. Data Logging

4. Settings

5. Sensors

6. (Empty)

7. End Point Detection

8. Help

Moving between Menu Screens

You can move between menu screens by either touching the menu icons or
swiping across the screen from left to right or right to left.

The Following system parameters are displayed in the center portion of the screen:

Function Indicator Bar

A thin blue indicator bar will appear under the icon of the menu screen that is
currently being displayed. The same type of indicator bar will appear above the
operation buttons to signify if each function is ON or OFF.

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System Parameters Display

Line 1

COLLECTOR, VACUUM & TEMP HEADING – Describes the

measurement that is displayed directly below the text.

Line 2

COLLECTOR MEASUREMENT - Displays the temperature of the

collector coil in °C or °F (as selected in Settings). The value displayed
will be RED if the temperature is > -60° C. The value displayed will be

GREEN if the temperature is ≤ -60° C.

VACUUM MEASUREMENT – Displays the Freeze Dryer vacuum level
in mbar, Pa, or Torr (as selected in Settings). The display will read “High”
if the vacuum level is > 5 mbar. If the vacuum level is ≤ 5 mbar, a
numerical value will be displayed. The value displayed will be RED if the
vacuum is > 1.500 mbar. The value displayed will be GREEN if the
vacuum is ≤ 1.500 mbar.

SHELF & SAMPLE TEMPERATURE MEASUREMENT – Displays
the shelf & sample temperatures in °C or °F (as selected in Settings).

Line 3

Vacuum Set Point – Displays the current vacuum set point in mbar, Pa, or

Torr (as selected in Settings) in the center section Line 3.

Line 4

Operation Mode – Indicates the current status of the Freeze Dryer. One of

the following will always be displayed on this line:

1. Standby – Collector and Vacuum are both OFF

2. Manual – Collector and/or Vacuum are ON, but no Program is

currently running.

3. Program – If a program is currently running this line will

display the actual Program Name, Step #, and Time Remaining
(hh:mm) in the current step of the program.

4. Defrost – Defrost will be displayed when the hot gas defrost

function is turned ON.

Line 5

Data Logging Status – On or Off

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Operation Buttons

Operation buttons are displayed across the bottom of the Home screen.

 PROGRAM – This button opens the PROGAMS screen described in the

next section.

 MANUAL – Use this button to turn the collector & shelf control ON/OFF.

There are 3 different modes to choose from within the MANUAL function:

o Max Cold – This setting allows the shelf to be used to freeze

samples below -55°C, obtaining “Max Cold” (MC) temperature of
approximately -75°C. Max Cold should only be used for pre­freezing samples, not for freeze drying, since the collector coil is
not being cooled in this mode.

o In-Flask – This setting is used to freeze dry pre-frozen samples

when using ONLY the sample valves on the left side of the unit.
There is no shelf temperature control in this mode.

o Shelf Temp Set Point – When using this mode the shelf

temperature will maintain the set point selected. The shelf
temperature may be set in the range from +50°C to –55°C in 1°C
increments.

 VACUUM – Use this button to turn the vacuum pump ON/OFF or change

the vacuum set point value.

 DEFROST – Use this button to turn the defrost mode ON/OFF. The

defrost function will turn off automatically if the collector temperature
reaches +60°C or if defrost has been running for 2 hours.

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Programs Screen

The Programs screen will allow you to create and store up to 30 programs with up

to 16 segments per program. When no program has been selected the “New”

button will be the only active button along the bottom of the screen. A program
can be selected from the program list by pressing the program name. When a
program has been selected, the program row will be highlighted (blue). With a
program selected, the rest of the buttons along the bottom of the screen will
become active (Start, Edit/View, Copy & Delete).

 New – Use this button to add a new program to the program list. The new

program name will be defaulted to a two digit sequential number (starting
with “Program 01”) that will be incremented as new programs are added to
the list (i.e. Program 02, Program 03…). The default name can be edited as
desired in the New or Edit/View screen. Up to 30 programs can be saved.

 Start/Stop – Use this button to start or stop a program. The wording on

this button will change from “Start” to “Stop” depending on whether or not

a program is currently running.

 Edit/View – Use this button to Edit or View the parameters of a saved

program.

 Copy – Use this button to Copy a selected program. The default name will

be “Copy of Program Name”. The program name can be edited as desired.

 Delete – Use this button to delete a saved program from the list.

When a program is currently in progress, a “running man” icon will appear to the

left side of the program name. If the running program is selected (highlighted), the
“Stop” button will be active, and the “Delete” button will be inactive.

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“New” program

When the “New” button is selected, the following screen will be displayed.

Pressing the program name field will allow you to edit the program name.
The Step numbers are listed in numerical order. Ramp Rate, Shelf Temp., Time,

and Vacuum can be edited by pressing any of these cells. The “Add” button will
add a new Step to the program. Pressing the “remove” button will delete the

selected step from the program. Changes will not be saved until the “Save” button

is pressed. Pressing the “Back” button will return to the Programs screen without

saving changes.

 Ramp Rate – This allows the temperature of the system to be increased or

decreased at any desired rate within the capacity of the heating and cooling
systems of the Freeze Dryer. Without samples on the shelf, the system is
capable of cooling at a rate of approximately 0.5°C/min from 50°C to 0°C,
.25°C/min from 0°C to -55°C, and can heat at approximately 3°C/min.
Ramp Rate may be set anywhere from 0.05°C to 3.0°C/min.

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Original instructions

 Shelf Temp. - May be set anywhere from +50°C to –55°C in 1°C

increments. There is also a Max Cold (MC) setting that allows the system
to reach its lowest possible temperature (approximately -75°C). Max Cold
should only be used for pre-freezing samples, not for freeze drying, since
the collector coil is not being cooled in this mode.

 Time – This time represents how long the system will hold at the Shelf

Temp. that has been programmed for each Step. The time can be set from
00:01 to 99:59 (1 min to 99 hrs 59 min). It can also be set to an indefinite
amount of time, which is represented by the infinity symbol (∞).

 Vacuum – Vacuum control can be set from 0.000 to 1.500 mbar.

Illustration of a simple 3 Step Program

Data Logging Screen

If no data log file has been selected the “New” button will be the only active
button along the bottom of the screen. A data log file can be selected from the list
by pressing the data log file name. When a data log file has been selected, the row

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Original instructions

will be highlighted (blue). With a data log file selected, the rest of the buttons
along the bottom of the screen will become active (Data View, Chart, Copy &
Delete). If a data log file is currently running (collecting data), the “running man”
icon will appear to the left of data log file name. If the currently running data log
file is selected (highlighted), the Copy & Delete buttons will be inactive.

 New/Stop – Use this button to start a new data log file or to stop a running

data log file. The wording on this button will change from “New” to

“Stop” depending on whether or not a data log file is currently running.

The new data log file name will be defaulted to a three digit sequential

number (starting with “Batch 001”) that will be incremented as new data

log files are added to the list (i.e. Batch 002, Batch 003…). The default

name can be edited as desired in the New or Data View screen.

 Data View – Use this button to view the data from a saved data log file in

table format.

 Chart – Use this button to view data from a saved data log file in chart

format (1 temperature sensor & 1 vacuum sensor can be graphed at a time).
Temperature and vacuum sensors can be selected from pop-up menus.

 Copy – Use this button to copy a selected data log file to a USB flash drive

or computer via the USB port on the left side of the Freeze Dryer.

 Delete – Use this button to delete a saved data log file from the list.

Settings Screen

Many of the user preferences may be configured from the Settings menu.
Before operating the freeze dryer you may want to configure some of the available
preferences. There are nine categories in the Settings menu.

1. Language – Chose from 5 languages: English, Spanish, French, German,

Italian

2. Time/Date – Chose Clock Format (12 or 24 hour), Set Time, Set Date for

manually setting the time and date. The Auto Update time & date feature can
be used if a valid network connection has been established. With Auto Update
the following settings need to be entered:

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Original instructions



 Time Zone – Enter the Greenwich Mean Time (GMT) offset value for

your location (integer value between -12 & +12).

 NTP server – Enter the “web address” (URL) of the NTP server (of

your choice) that will provide clock synchronization data to the Freeze
Dryer via the Ethernet connection.

 Daylight Savings (ON/OFF) – This feature can be used to adjust the

time for areas observing Daylight Savings Time (DST).

3. Units – Select your desired unit of measure for Temperature (°C , °F) and

Vacuum (mbar, Pa, Torr)

4. Security – A four digit security code can be set that will require users to enter

the code to gain access to selected screens. A security code will always be
required to enter the “Security” screen.

THE FACTORY DEFAULT SECURITY CODE IS “1234”

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 System – If the System security feature is selected (box checked),

users will be required to enter the security code to gain access to the
touch screen controls when the screen is reactivated from a screen
timeout (sleep mode) or power failure (screen timeout described in
the Settings/Display section).

 Programs, Email Alerts, Calibration, Network – Users will be

required to enter the security code to gain access to each individual
screen that has been selected (box checked).

5. Alerts – If a valid network connection has been established (via the Settings /

Network screen) and Email Settings have been properly configured, alerts can
be emailed to selected users, by entering the appropriate email address(es) and
selecting the type of alerts that will be sent to each email address.

The Edit & Delete buttons will be inactive until an email address is selected
from the list. When an email address has been selected, it will be
highlighted (blue).

 Add – Use this button to add a new email address to the list.
 Edit – Use this button to edit an existing email address.

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Original instructions

 Delete – Use this button to delete an existing email address.
 Device Name – The default name for this field will be “Labconco

Freeze Dryer”. The name can be edited by pressing the name field.

 Configure Email Settings – Pressing this button will bring up the

following screen, where the appropriate email settings can be
entered.

If you do not know the proper information to enter into each of the SMTP
fields, consult your IT personnel or email service provider.

After entering the appropriate information in all of the SMTP fields, press
the Save button. The system will check the information entered into each
field to make sure each entry is valid. If one or more invalid entries are
found the following message will appear: EMAIL SETTINGS ERROR –

Error connecting to email server, check your settings.

If all the SMTP fields entered are valid, but the network connection is not
working (or Ethernet cable is not connected) the following message will
appear after pressing the Save button: EMAIL SETTINGS ERROR –
Network connection is not active

 Test Email – After the Email Settings and Network Settings have

been correctly entered, pressing this button will send a test email to
the selected email address.

 Select Alerts – The type of alerts that will be sent to each

individual email address can be customized. Pressing the Select
Alerts button will bring up the following selection list. Alerts can
be selected or deselected by pressing the check box in front of each
alert. Scroll list up or down by swiping screen up or down.

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6. Maintenance – This screen contains data and settings pertaining to the

operation hours and maintenance period for the vacuum pump and refrigeration
system. Scroll up and down to view the entire page.

 Line Voltage Offset - These offset values determine the acceptable voltage

range for electrical power supplied to the Freeze Dryer. If the supply line
voltage is outside of the acceptable range, then the Line Voltage Range alert
will be displayed. The default range is +/- 10% of the nominal rated voltage
(115V or 230V depending on model). The LOW Line Voltage Offset can be
adjusted from 0 to -10V (115V models) or 0 to -20V (230V models). The
HIGH Line Voltage Offset can be adjusted from 0 to +10V (115V models) or
0 to + 20V (230V models). Scroll up and down to view the entire page.

 Vacuum Pump Protection – If vacuum pump protection is turned ON, the

vacuum pump will automatically be turned off if a “Vacuum Leak” alert is
presented. This will protect oil filled pumps from losing their oil and
potentially damaging the pump in the event of a major vacuum leak. The

factory default setting for vacuum pump protection is ON, and it is highly
recommended that it remain in the ON setting. If vacuum pump protection

is turned OFF, the pump will continue to run after a “Vacuum Leak” alert is
presented (i.e. there is no protection for the pump in the event of a major
vacuum leak).

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Original instructions

Sample

7. Calibration – Some sensors in the Freeze Dryer can be calibrated. This

process should be done by a qualified technician or metrologist with reference
sensors that are known with certainty. An external reference sensor must be
placed at the same measurement point (as close as physically possible) as the
sensor being calibrated. The Collector and Shelf temperature sensors have
their own reference thermocouple (type T) built into each sensor. Each
thermocouple is a pair of wires (red & blue) sheath with a brown outer casing.
They are located inside the housing of the Freeze Dryer and can be accessed by
removing the back panel. A thermocouple meter can be used to read the
thermocouple value as a reference value.

 Current Reading (uncorrected) – This gives the raw sensor reading

with no offset applied to the reading.

 Offset – This is the value that will be added to the Current Reading

(uncorrected) to give the proper adjusted reading that matches the
reference sensor being used for calibration. The adjusted reading
(uncorrected + Offset) is the value that will be presented on all screens
outside of the Sensor Calibration screen. Pressing the Offset field of
the desired sensor will bring up a dialog box for entering the offset
value for the selected sensor.

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Original instructions

CALIBRATION

Offset: ________

Cancel

Calibrate

 Calibrate - Pressing this button will save the entered offset value.

Temperature sensor offset values can range from -5 to +5° C.
Vacuum Sensor offset values can range from -0.020 to +0.020
mbar.

8. Display – This screen contains settings for controlling various functions of the

touch screen display.

 Brightness Slider – Moving the slider to the right will increase the

brightness level, moving to the left will decrease the brightness level
of the display.

 Touch Sounds – With Touch Sounds turned ON, there will be an

audible sound each time the display is touched. Pressing the Touch
Sounds button will toggle between ON & OFF.

 Screen Timeout Function – If this function is turned ON, the screen

will go into low power mode (sleep) when no screen touches have
been detected for the allotted set time (Screen Timeout). Pressing the
Screen Timeout Function button will toggle between ON & OFF.

 Screen Timeout – This value can be set to 5, 10, 15, 30 min, 1, 2, or 3

hours.

9. Network – If the DHCP Enabled button is turned ON, and an Ethernet cable is

connecting the Freeze Dryer to an active network, then the IP Address, Subnet
Mask, and Default Gateway fields will be populated automatically. If the
DHCP Enabled button is turned OFF, the IP Address, Subnet Mask, and

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Original instructions

Default Gateway fields can then be manually entered to establish a network
connection. If you do not know the proper information to enter into each of the
Network fields, consult your IT personnel.

Sensors Screen

This screen contains a list of all sensors currently installed on the Freeze Dryer and
any attached accessory. The Sensors screen is for viewing only (there are no
selectable fields on this screen).

End Point Detection Screen

If the communication cable is NOT connected or if NONE of the vacuum sensors
are connected to the End Point Detection Enclosure, the End Point Detection icon
(checkered flag) will NOT appear in the menu bar. If the End-Zone end point
detection accessory is properly connected to the Freeze Dryer, this screen will be
used to control the End Point Detection settings for up to five individual freeze dry
flasks.

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Original instructions



 End Point Detection status – Shows the current status for each flask.

The status will be one of the following: ON, OFF or COMPLETE.
Touching the end point detection cell for any given flask will allow you
to turn the end point detection ON/OFF. The End Point Detection
status will display COMPLETE automatically when an End Point
Detection alert is achieved for any given flask.

 Offset from Ref. Vacuum – This allows the user to determine the level

of dryness at which the system will declare that the primary drying end
point has been reached. The offset from Ref. Vacuum represents the
difference between the Reference vacuum sensor and the individual
flask sensor. This value can be set from 0.010 to 0.050 mbar (0.010
being the most dry & 0.050 being the least dry).

END POINT DETECTION INDICATES THE END OF THE PRIMARY
DRYING PHASE. SOME SAMPLES MAY REQUIRE ADDITIONAL
FREEZE DRYING TIME (SECONDARY DRYING PHASE) TO
ACHIEVE THE DESIRED MOISTURE CONTENT.

Chapter 4: Lyo-Works Operating System

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Original instructions

Help Screen

 Diagnostics – This screen will give information about the power supply

to the Freeze Dryer (Voltage, Current & Frequency) and allow the user
to run Manual or Auto Diagnostics on the unit.

 User’s Manual – Go to www.labconco.com for user’s manual
 About – Displays the catalog number of the Freeze Dryer and the

software versions currently installed.

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Original instructions

CHAPTER 5

USING YOUR FREEZE DRYER

Operation Checklist

The following checklist should be followed prior to each use of your Freeze Dryer:

1. Wipe out the interior of the chamber with a soft cloth or paper towel to remove

any moisture or debris.

2. Check the collector tray drain hose to ensure that the hose is free of moisture

and that the drain plug is securely installed.

3. Using a soft, lint-free cloth or paper towel, wipe the door gasket to remove any

dirt and contaminants that could cause a vacuum leak. Vacuum grease is not
required on the door gasket.

4. Check that each sample valve is closed or in the “vent” position. See Figure 4.

Vacuum Pump Ballast Setting

Most vacuum pumps are equipped with a gas ballast mechanism. The freeze dry
process requires high vacuum. Therefore, it is recommended that the gas ballast be
closed during the operation of the Freeze Dryer.

Note: If the gas ballast is left open for extended periods of operation, the oil can

be pumped out the exhaust, causing the pump to fail.

Sample Freezing

Before the freeze dry process can occur, the products to be dried must be in a
frozen state. This can be accomplished in a freezer separate from the freeze dryer
or on the shelf inside the Freeze Dryer. If the shelf inside the Freeze Dryer will be
used to pre-freeze samples, make sure that the vacuum is turned off until the
samples are fully frozen.

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Original instructions



THE VACUUM SHOULD ALWAYS BE TURNED OFF WHILE
USING THE SHELVES TO FREEZE SAMPLES

The sample container volume should be at least two to three times greater than the
sample volume. The temperature required for pre-freezing is dependent on the
characteristics of the sample. Pre-freezing temperature is typically at least 10° to
20°C below the eutectic or collapse temperature of the sample. Pre-freezing on the
Triad shelf can be done in Manual or Program modes as long as the shelf set point
temperature is adequate to fully freeze the sample.

In MANUAL mode, simply set the Shelf temperature to the desired value for
freezing the sample, and allow an appropriate amount of time for the sample to be
fully frozen before starting the vacuum and the freeze drying process.

In PROGAM mode, the vacuum set point can be programmed to OFF for the
initial pre-freezing step. If multiple steps are desired for more complex pre­freezing/annealing processes, then program the vacuum set point to OFF for each
of the pre-freezing / annealing steps.

If desired, place the sample probe in a sample vial to monitor the sample
temperature throughout the pre-freezing & freeze dry process.

Shelf Loading

The stoppering mechanism is capable of generating a very strong force, which
could damage the shelf or mechanism. Therefore, it is important to distribute the
serum bottles to be stoppered evenly across the entire surface of the shelf. When
stoppering vials, always place a vial on each corner of the shelf.

Setting the Operating Vacuum Level

To change the Vacuum Set Point

1. Go to Home screen

2. Press VACUUM,

 enter new Vacuum Set Point value
 press APPLY (or START if vacuum pump is not already running)

The vacuum level may be set by the user to optimize the freeze dry process.
Normally, the sublimation rate will increase as the pressure increases in the Freeze
Dryer. A good starting place is to set the vacuum so its level is equivalent to about
10°C colder than the eutectic or collapse temperature of the sample. The reference
table below shows the relationship between ice temperature and vapor pressure.

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Temp.

(°C)

Pressure

(mbar)

Temp.

(°C)

Pressure

(mbar)

Temp.

(°C)

Pressure

(mbar)

Temp.

(°C)

Pressure

(mbar)

Temp.

(°C)

Pressure

(mbar)

0

6.11

-11

2.37

-21

0.94

-31

0.34

-50

0.039

-1

5.63

-12

2.17

-22

0.85

-32

0.31

-60

0.011

-2

5.18

-13

1.98

-23

0.77

-33

0.28

-70

0.003

-3

4.76

-14

1.81

-24

0.70

-34

0.25

-80

0.001

-4

4.37

-15

1.65

-25

0.63

-35

0.22

-5

4.02

-16

1.50

-26

0.57

-36

0.20

-6

3.69

-17

1.37

-27

0.52

-37

0.18

-7

3.38

-18

1.25

-28

0.47

-38

0.16

-8

3.10

-19

1.13

-29

0.42

-39

0.14

-9

2.84

-20

1.03

-30

0.38

-40

0.13

Material

Solidification/Eutectic

Temperature

Pre-Freeze

Temperatures

Vacuum Set Point

Bacteria, Virus

≤ -40°C

≤ -50°C

≤ 0.04 mbar

Milk

-5 to -13

-15 to -23

1.65 to 0.77

Fungi

≤ -40

≤ -50

≤ 0.04

Vegetable Tissue

-25 to -50

-35 to -60

0.22 to 0.01

Human Tissue

-30 to -40

-40 to -50

0.12 to 0.04

Blood Plasma

-10 to -25

-20 to -35

1.03 to 0.22

Vaccine

-30 to -40

-40 to -50

0.12 to 0.04

Vapor pressure above ice

Adjustments to the vacuum level must be made for various freeze drying
conditions. Factors that must be considered are whether the sample is freeze dried
on heated shelves or in glassware attached to sample valves, the volatility of the
sample itself, the size of the sample and the heat energy supplied to the sample.
When the vacuum control is set to operate at less vacuum (higher pressure), the ice
holding capacity of the collector may be decreased. Some guidelines for setting
the vacuum level are shown below. Exact protocols must be determined by the
user for the specific samples that are being freeze dried.

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Original instructions

Freeze Drying Inside the Chamber

The following procedure should be followed when freeze drying using the
temperature controlled shelf inside the vacuum chamber.

Manual Mode with Pre-frozen Samples

1. Go to the Home Screen and press MANUAL

2. Enter the desired shelf set point temperature and press Start.

3. The system will begin ramping the shelf temperature until the set point

temperature is achieved. After reaching the set point temperature, the
system will hold at this temperature indefinitely.

4. When the collector temperature reaches –80°C and the shelf

temperature reaches the desired set point, turn the vacuum pump off,
release the vacuum, and open the chamber door. Place the frozen
samples on the shelf, close the door, and turn the vacuum on.

5. Shelf set point temperature and vacuum set point can be changed at any

time during the manual freeze dry process.

6. To stop, press MANUAL, then Stop. This will turn off the refrigeration

system and heater.

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Original instructions



Manual Mode with Unfrozen Samples

1. Place unfrozen samples on the shelf and close the door. The sample probe

can be placed in the sample if desired.

2. Start the refrigeration system by pressing MANUAL, then select Max Cold

or enter a Shelf Temp Set Point (depending on the desired shelf
temperature), then press Start.

3. Make sure the vacuum is turned OFF.

THE VACUUM SHOULD ALWAYS BE TURNED OFF WHILE
USING THE SHELVES TO FREEZE SAMPLES

4. The shelf will cool to the entered set point value (or approx. –75°C in Max

Cold mode).

Note: With the system starting at room temperature, it will take
approximately 6 hours to freeze a 2 liter tray of water in Max Cold
mode.

5. When the sample is frozen, change the shelf set point to the desired

temperature.

6. Turn on vacuum.

7. Shelf set point temperature and vacuum set point can be changed at any

time during the manual freeze dry process.

Program Mode with Pre-Frozen Samples

The shelf should be pre-cooled to the desired temperature before placing pre­frozen sample on it for freeze drying. Pre-cooling the shelf can be done with either
the Manual or Program mode (Manual mode has been detailed above). You can
access the Programs screen from two different buttons, both lead to the same
location:

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Original instructions



1. Go to the Programs screen.

2. Select a program from the saved programs list, or create a new program.

3. With the desired program selected from the list, press the START button.

4. The refrigeration system will start.

5. When the collector temperature reaches –80°C and the shelf temperature

reaches the desired set point of segment 1, turn off the vacuum pump and
release the vacuum. Place the frozen samples on the shelf. Close the door
and turn the vacuum on.

Program Mode with Unfrozen Samples

1. Place unfrozen samples on the shelf and close the door. The sample probe

can be placed in the sample if desired.

2. Go to the Programs screen.

3. Select a program from the saved programs list, or create a new program.

The first step of the selected program (or multiple steps if desired) must
have the vacuum turned OFF while the sample is pre-freezing.

THE VACUUM SHOULD ALWAYS BE TURNED OFF WHILE
USING THE SHELVES TO FREEZE SAMPLES

4. With the desired program selected from the list, press the START button.

5. The refrigeration system will start.

6. The program should contain all the necessary steps to take the sample

through the pre-freezing, primary drying, and secondary drying processes.

Stopping the Freeze Dry Cycle

Manual Mode

1. Go to the Home screen, press MANUAL, then Stop.

2. Turn OFF the vacuum pump.

3. Position the “Vac Release” valve to OPEN (or open one of the sample

valves on the left side of the unit).

4. Allow the vacuum to bleed to atmospheric pressure, then open the door

and remove the samples.

5. Defrost the ice, drain the collector pan, and dry.

Program Mode

1. If operating in program mode, the “Program Complete” alert will be

displayed on the touch screen at the end of the last programmed step.

After the “Program Complete” alert has been presented, the unit will

continue to run in Manual mode until turned off by the user (shelf set
point temperature and vacuum set point will hold the settings from the
last program step).

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Chapter 5: Using Your Freeze Dryer

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Original instructions

2. To stop a program (at any time) go to the Programs screen, select

(highlight) the program that is currently running, press Stop. This will
turn OFF the refrigeration system & vacuum pump.

3. Position the “Vac Release” valve to OPEN (or open one of the sample

valves on the left side of the unit).

4. Allow the vacuum to bleed to atmospheric pressure, then open the door

and remove the samples.

5. Defrost the ice, drain the collector pan and dry.

Freeze Drying Using Sample Valves (In-Flask Mode)

The In-Flask mode is used to freeze dry pre-frozen samples when using ONLY the
sample valves on the left side of the unit. There is no shelf temperature control in
this mode. The refrigeration system will only cool the collector. The following
procedure should be followed when using the sample valves to freeze dry:

1. Go to the Home screen and press MANUAL, then select In-Flask.

2. Once the collector temperature is less than –80°C and vacuum is less than 0.133

mbar, connect a pre-frozen sample to a sample valve on the left side of the cabinet

using an adapter. Turn the plastic valve knob to the “VACUUM” position to

open the valve. The bevel on the knob should be positioned toward the sample
port to apply vacuum to the sample.

3. Before adding another sample, allow system vacuum to return to the

vacuum set point. Any combination of valves and sample sizes may be
utilized at one time provided that the system vacuum and collector
temperature remain sufficiently low to prevent melting of the frozen
sample.

4. When all the frost has disappeared from the outer surface of the sample

container and no cold spots can be detected by handling the container,
primary drying is nearly complete. To be certain of low final moisture
content, dry the sample for several hours past this point.

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Chapter 5: Using Your Freeze Dryer

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Original instructions

5. To remove a container after freeze drying is complete, turn the plastic knob

on the valve to the “VENT” position, which closes the valve and vents the
container. Should backfilling with an inert gas be required, connect the gas
supply line to the vent port on the valve before turning the plastic knob on
the valve to vent position. The sample container may now be removed. In
the vent position the bevel on the valve knob should point away from the
sample port.

6. Ampules may be flame sealed while connected to a valve by using a

sealing torch. Care must be taken not to burn the valve. An insulation
material placed between the valve and the torch is recommended.

Stoppering Vials

The stoppering operation (when desired) is performed after the freeze dry process
is complete and before breaking vacuum. To seal vials, move the Stoppering
control toward the “LOWER” position. This action allows the diaphragm above
the stoppering platen to inflate causing the platen to lower. The platen will lower
until it contacts the vials on the shelf. The rubber stoppers will be pressed into the
vials. Monitor the stoppering process by looking through the chamber door.

When all of the vials appear to be stoppered, move the Stoppering Control to the

“RAISE” position. This opens the diaphragm to the vacuum pump, which deflates

the diaphragm. Opening the Vacuum Release Control also deflates the stoppering
diaphragm. The Stoppering Control should be left in the “RAISE” position when
not stoppering. The stoppering mechanism is equipped with anti-tilt switch which
will stop the platen from lowering if it tilts more than 4°: If this happens return the
stoppering control valve to the “RAISE” position. This will deflate the diaphragm
and allow the platen to return to the raised position. Determine the cause of the tilt
condition before proceeding.

Vacuum Break/Backfilling

To open the chamber door, the vacuum must be released. To release the vacuum,

move the Vacuum Release Control to the “OPEN” position and turn off the

vacuum pump by pressing VACUUM, then Stop. The vacuum can also be
released more quickly by opening one of the sample valves on the left side of the
unit. Air enters the vacuum chamber through the Back Fill Port. When the sound
of air through the Back Fill Port is no longer audible, the chamber door is ready to
open.

To backfill the chamber with a gas, connect a cylinder of the desired gas to the
Back Fill Port. The port will accept 1/8" tubing. The bottle must be equipped with
a regulator set to 15 psi maximum. The gas can be metered with the Vacuum
Release Control and chamber pressure monitored with the freeze dryer vacuum
gauge. Allow the vacuum pump to run for a few minutes while the gas enters to
permit the back fill gas to surround the freeze dried sample. When the vacuum
pump is turned off, air will enter the system through the vacuum break valve
located between the vacuum pump and the collector chamber.

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Original instructions

!

Defrosting

After the freeze dry process is complete the ice that has accumulated on the
collector coil must be removed. The Triad Freeze Dry System is equipped with a
rapid defrost feature.

The following procedure should be followed when defrosting the collector coil:

1. Press the DEFROST button on the Home screen. The defrost indicator

above that button will illuminate. Allow unit to operate in this condition
until all condensate is defrosted from the collector coil. Turn the system off
by pressing DEFROST, then Stop. The defrost function will turn off
automatically if the collector temperature reaches +60°C or if defrost has
been running for 2 hours.

2. Place the drain hose in a suitable container to collect the condensation.

Remove the drain plug from the drain hose.

3. Replace drain plug and remove drain pan to dispose of the ice and wipe

dry.

4. Wipe any excess liquid from inside of vacuum chamber, replace drain pan.

Utilization of acid requires immediate cleaning and
neutralization after defrost or physical damage to the collector
chamber and coil will result.

Do not attempt to chip ice from the collector coil as damage may
occur.

Never attempt to start the vacuum pump when there is liquid in
the collector chamber. This could result in damage to the vacuum
pump.

Alerts

A number of events may occur during a lyophilization procedure that will cause an
alert to be displayed on the Freeze Dryer touch screen. An audible alarm (beeper)
will also be sounded that will automatically be muted after one minute.
The specific alert type can be identified by observing the message box on the
display. The alert message box and audible alarm can be dismissed by pressing the
“Back” button on the alert message box. The following conditions will initiate an
alert.

Power Fail

If a power failure occurs during a Freeze Dry process, the vacuum control valve
will allow air to bleed into the Freeze Dryer System. If the failure is of a short
duration and the collector does not warm up above -30°C, when power is restored
the Freeze Dryer will restart and resume operation of the refrigeration and vacuum
systems. If the power failure lasts for a longer duration and the collector warms
above safe limits, when the power is restored, the Freeze Dryer will not

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Chapter 5: Using Your Freeze Dryer

42

Original instructions

automatically restart. This prevents melted sample from being drawn into the
collector and prevents liquid from being drawn into the vacuum pump.
When power is restored, the POWER FAIL alert message will be displayed on the
touch screen and the audible alarm will sound.

Line Voltage Range

If the voltage supplied to the Freeze Dryer varies beyond allowable limits, the
LINE VOLTAGE RANGE alert will be activated. The high and low alarm points
are preset at the factory to correspond to the normal allowable voltage variations
based on the nominal voltage specified for the freeze dryer. Some models may be
operated outside the normal voltage limits (see Appendix C). If necessary, the high
and low LINE VOLTAGE OFFSETS may be adjusted from the Settings /
Maintenance screen.

Collector Temp

After the collector temperature has cooled to below -40°C, an alert will be
activated if the collector temperature rises above -40°C for more than 5 minutes.

Service Vacuum Pump

The vacuum pump normally plugs into the vacuum pump electrical receptacle on
the back of the Freeze Dryer. When the Freeze Dryer has accumulated a total of
1000 operating hours, an alert will be activated. It may be necessary to service the
vacuum pump more frequently than every 1000 hours depending on the operation
of the Freeze Dryer. The pump oil should be regularly monitored to verify that
it is clean. The “Set hours between oil change” can be reprogrammed from the
Settings / Maintenance screen for any value from 0 to 9999 hours.

Vacuum Leak

If the vacuum level in the freeze dryer has not reached at least 5 mbar within 30
minutes of starting the vacuum, an alert will be activated and the vacuum pump
will be turned off (if it is plugged into the Freeze Dryer vacuum pump outlet).

System Temp Variation

Once the system temperature has stabilized (held set point temperature within +/­2°C for 20 minutes), if the manual set point temperature or program hold
temperature varies more than ±2°C from the set point (as measured by the system
temperature sensor), the “System Temp Variation” alert will be displayed.

Vacuum Variation

Once the system vacuum has stabilized at a point where it changes less than 0.020

mbar in 5 minutes, if the vacuum changes more than 0.500 mbar, the “Vacuum

Variation” alert will be displayed.

Vacuum Set Point

If the system is unable to achieve the vacuum set point (during Manual or Program
mode), an alert will be activated. This alert will be activated if the vacuum level is
not within 0.500 mbar of the vacuum set point in 20 minutes.

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Original instructions

Shelf Temp Set Point

If during a Ramp segment the shelf temperature stabilizes without reaching the
Shelf Temp Set Point, the control will automatically enter the next Hold segment
and the “Shelf Temp Set Point” alert will be displayed.

Platen Tilt

If the stoppering platen tilts more than 4° while stoppering, the platen will stop. If

this happens return the stoppering control valve to the “RAISE” position. This will

deflate the diaphragm and allow the platen to return to the raised position.
Determine the cause of the tilt condition before proceeding.

Program Complete

This alert will be activated when the last step of a running program has been
completed.

Status Email Messages

If this option has been turned on, the selected email addresses will receive
STATUS alert messages containing the current operating parameters of the Freeze
Dryer. Status alert messages will not be displayed on the touch screen. The
Freeze Dryer can be configured to send email status alert messages from the
Settings / Alerts / Select Alerts screen.

Copy (Export) a Data Log File

Data Log Files can be copied (exported) to a USB flash drive via the USB port on
the left side of the Freeze Dryer. The file will be exported as a “comma separated

values” file (.csv file extension), which can be easily opened with a spreadsheet

application program for data analysis and graphing. To copy a file:

1. Insert USB flash drive into the Freeze Dryer USB port

2. Go to Data Logging screen

3. Select the file that you want to copy by pressing the Data Log File name

4. Press the Copy button

Downloading Data Log Files via Ethernet

Data log files that are stored in the Freeze Dryer can be downloaded via an
Ethernet connection. In order for this to be possible, a working Ethernet
connection must first be established. The following steps must be completed:

1. An Ethernet cable must be connected to the Ethernet port on the back of the

Freeze Dryer and to a Local Area Network (LAN) with working internet
service.

2. The proper settings must be entered into the Settings>Network screen to

establish a valid network connection (see description of Network screen in
Chapter 4).

3. Write down the “IP Address” shown on the Settings>Network screen of the

Freeze Dryer (this number will be used in Step 5)

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Chapter 5: Using Your Freeze Dryer

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Original instructions

4. Open an internet browser of your choice from a computer that is connected

to your LAN.

5. Type the following into the address bar at the top of the browser screen:

http://“IP Address”:8080 where “IP Address” represents the number
written down from the Settings>Network screen in Step 3 (example =

http://10.10.9.117:8080).

6. The following webpage will appear with a list of all the Data Log files that

are stored on the Freeze Dryer.

7. Single click on a file name from the list to download the file.

8. Single click on the downloaded file to open the file in Excel.

Copy a Program

If you wish to duplicate a program or quickly create a program that is similar to an
existing program, you can use the copy program function. The new program file
created by the copy function will be stored on the Freeze Dryer (programs cannot
be copied to a USB flash drive or computer). To copy a program:

1. Go to Programs screen

2. Select a program from the list by pressing the program name

3. Press the COPY button

4. Edit program name if desired (the default program name will be “copy of

program name”)

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Original instructions

!

CHAPTER 6

MAINTAINING YOUR FREEZE

DRYER

Service Safety Precautions

 Always ensure that only authorized technicians service the refrigeration,

vacuum and electrical systems.

 If performing any electrical maintenance, always disconnect the power at

the main disconnect.

 If the refrigeration system must be operated with access panels removed

(for diagnostic purposes only), stay clear of moving fan blade.

 Always practice team lifting when moving heavy equipment.
 After servicing, verify that all access panels or covers are in place before

resuming normal operation of the equipment.

Routine Maintenance Schedule

Under normal operation, the Freeze Dryer requires little maintenance. The
following maintenance schedule is recommended:

As needed:

1. The user has the responsibility for carrying out appropriate

decontamination if hazardous material is spilled on or inside the equipment.
This may be done by wiping the contaminated surfaces with a soft cloth
dampened with alcohol. Alcohol may craze the acrylic door. Before using
any cleaning or decontamination method except those recommended by
Labconco, users should check with Labconco to determine that the
proposed method will not damage the equipment.

2. Clean up all spills; remove liquids from the chamber.

3. Clean door and gasket using soft cloth, sponge or chamois and a mild, non-

abrasive soap or detergent.

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Chapter 5: Using Your Freeze Dryer

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Original instructions

!

4. Check oil level of the vacuum pump. It should be between MIN and MAX.

If the oil level is less than an inch (25.4 mm) above MIN, add oil to proper
level.

5. If oil shows cloudiness, particles or discoloration, drain the pump and

replace with fresh oil.

6. The use of acids requires immediate cleaning and neutralization after a run

or physical damage will result.

7. When freeze drying biological substances, it may be necessary to

decontaminate the system. A surface decontaminant should be used to
clean the accessible surfaces.

The use of ethylene oxide is not recommended because of its
hazardous and corrosive nature. Contact Labconco for
additional information.

Monthly:

1. The rubber components on the freeze dry system may eventually deteriorate

and require replacement. The effective life of rubber parts depends upon both
their usage and the surrounding environment. Check all rubber hoses and
gaskets and replace any that show signs of hardening, permanent set or
deterioration.

2. Using a soft cloth, sponge or chamois and a mild, non-abrasive soap or

detergent, clean the acrylic door.

3. Using a soft cloth, sponge, or chamois and a mild, non-abrasive soap or

detergent, clean the exterior surfaces of the cabinet. Liquid spray cleaners and
polishes may be used on the exterior surfaces. Do not use solvents to remove
stains from the exterior surfaces as they may damage the finish.

Annually:

1. Every 12 months, or more often if the freeze dry system is operated in a

dusty environment, the refrigeration system condenser should be cleaned.
Using a vacuum cleaner with brush attachment, clean the condenser to
ensure proper airflow for peak performance.

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47

Original instructions

CHAPTER 7
TROUBLESHOOTING

Refer to the following if your Freeze Dryer fails to operate properly. If the
suggested corrective actions do not solve your problem, contact Labconco for
additional assistance.

Vacuum System Performance

FreeZone Triad Freeze Dry Systems that are clean and dry and without
samples attached should reach a vacuum of 0.133 mbar within 30 minutes
and should achieve an ultimate vacuum of 0.040 mbar within 18 hours when
the refrigeration is operating. If the freeze dry system does not obtain a
satisfactory vacuum, perform the following maintenance tests.

Vacuum Pump

First make sure that the vacuum pump operates. If it fails to operate, check
the electrical connections of the Freeze Dryer to the power source and then
check the electrical connection of the vacuum pump to the Freeze Dryer. If
the vacuum pump has a power switch, make sure that it is turned on. Note:

If the vacuum pump is plugged directly into a wall outlet for
troubleshooting purposes, the vacuum START button must be initiated
on the display screen. If the vacuum START button is not initiated the
vacuum vent valve will remain open and the vacuum reading will remain
on HIGH. If the vacuum is not adequate when the vacuum pump is

operating, proceed with the following steps:

1. Check the oil and ensure it is clear and clean. If the oil looks cloudy

or has any particulates, replace the pump oil. Sometimes it may be
necessary to flush the pump with clean oil several times. To flush the
pump, run the pump 5 to 10 minutes to allow the oil to warm up.
Drain the oil and refill with clean oil. Repeat as necessary.

2. Check the oil level in the pump. Ensure it is filled to the correct level.

3. Check vacuum hose connections from the pump to the Freeze Dryer

and try running the unit.

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Chapter 7: Troubleshooting

48

Sample Valve Installation

If vacuum problems continue, consider obtaining a second vacuum
gauge capable of reading a vacuum of 0.010 mbar. It is often useful in
determining if the vacuum pump is operating properly and the vacuum
sensor reading is accurate.

4. Isolate the pump by disconnecting the vacuum hose from the Freeze

Dryer. Deadhead the pump by inserting the vacuum sensor from a
secondary vacuum gauge into the end of the vacuum hose and observe
the vacuum reading obtained. Confirm that the pump is capable of
achieving an ultimate vacuum less than 0.010 mbar or approximately
10 microns. If an inadequate vacuum reading is obtained, the pump
has most likely failed and may need to be replaced or rebuilt.

Gaskets, Tubing, Connections, Sample Valves

1. Check all sample valves on the side of the cabinet and ensure all

valves are closed or in the vent position.

2. Check all vacuum tubing for signs of deterioration or cracking.

3. Check all connections and make sure they are secure and leak tight.

4. Check the freeze dry system door gasket for indentations cracks or

tears. Clean gaskets using a soft, lint free cloth or paper towel.

5. The freeze dry system chamber and collector must be dry.

6. When checking the sample valves, pull as much vacuum as possible.

If a vacuum indication is displayed, wiggle or rotate the valves and
watch the gauge for any fluctuation. Fluctuations can show a
potential vacuum leak. If the valve seems to be in good condition,
remove the valve and apply a thin coat of vacuum grease to the stem
and the outside sealing surface of the valve body, and reinstall the
valve. If the valve still seems to be the source of the problem, remove
the valve and stopper the hole with a rubber stopper. Continue
checking the other valves.

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49

Nuts

Latch
Pawl

Door Adjustment

The following adjustments can be made if the door is not sealing properly
when vacuum is applied.

1. The latch can be adjusted to increase the force

between the door gasket and chamber sealing
surface.

a. Open the door.
b. Using two ½" wrenches, loosen the nuts

securing the latch pawl.

c. Move the latch pawl towards the door to

tighten the latch.

d. Tighten the nuts.

2. The door hinges can be adjusted to move the door in

towards the chamber sealing surface.

a. Removing the four screws that attach the back panel to the

side panel.

b. Close the vacuum release. Remove the knobs from the two

valves on the front.

c. Remove the lower front panel.
d. Remove the one screw on the left side of the control panel.
e. Remove the three screws attaching the right panel to the base

and remove the panel.

f. Turn on the vacuum pump. After the vacuum level reaches

approximately 5mbar, loosen the six screws on the door
hinges. Do not remove the screws.

g. Once the vacuum level reaches approximately 1mbar, tighten

the six screws.

Refrigeration Module Performance

In Max Cold mode under a no-load condition and an ambient temperature of
20°C, the freeze dry system refrigeration system can achieve a shelf
temperature of –75°C or lower within 4 hours.

If any repairs are required on the refrigeration module, please call Labconco.
Repairs should only be undertaken by a competent refrigeration technician or
through an authorized Labconco service agency.

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Chapter 7: Troubleshooting

50

Filling the Fluid System

1. Disconnect power cord from supply outlet.

2. Remove the rear cover to expose the marked back foam insulation.

Cut through the foam along the marked lines and remove center
cutout.

3. Remove the insulation and both caps from the fluid circulation valve.

4. Attach a flexible tube to the exposed port (service port). The port is

3/8" x 45° male flare.

5. Remove the fluid reservoir cap and place the opposite end of the

tubing into the fluid reservoir.

6. Fill the reservoir slowly allowing the fluid to drain through the

system. When the system appears full, prepare to start the circulation
pump.

7. Open the fluid circulation valve by completely “down-seating” (turn

top valve stem completely clockwise) to direct fluid to by-pass
through tubing to fluid reservoir.

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51

Name:

Lexol 542

Manufacturer:

Santa Barbara Chemical Co.
927 Indio Muerto
Santa Barbara, CA 93140
805-963-7793

!

8. Connect the unit to the proper power source. Activate the pump from the

display screen (Home screen) by selecting MANUAL, entering 24°C (in
Shelf Temp Set Point entry field), and pressing Start. This will start the
heat transfer fluid pump and also the refrigeration system. The fluid
system will circulate with the fluid going through the add-on tubing,
purging itself of air. Add fluid when needed to keep fluid in the reservoir.
After the fluid stream is established, tilt the entire freeze dry system up
and down. Observe the fluid stream and repeat the tilt procedure if it is
not free of bubbles. Fill reservoir to level indicated by the label.

9. Turn the pump and refrigeration system OFF by pressing MANUAL, then

Stop. Disconnect power cord from supply outlet.

10. Close “up-seat” fluid circulation valve (turn valve stem completely

counter-clockwise, closing the fluid/tubing by-pass).

11. Remove the tubing. Replace the caps on the valve and replace insulation

and covers. Replace the cap on the tank.

12. Replace insulation and seal air tight. Replace rear cover.

Heat Transfer Fluid Precautions

The fluid’s name and manufacturer are:

The fluid used for heat transfer in this system is combustible
and hazardous. Leaks and spills should be attended to
immediately or serious injury could occur.

Handling and Storage Precautions – Dirty Solvent: Store in

accordance with all applicable regulations. Tighten caps and store in
a cool area.

Precautions if Material is Released or Spilled: Spills should be
contained immediately. Spills may be soaked up with absorbent
materials, placed in closed containers, labeled, stored and disposed of
properly. Persons performing this work should wear adequate
personal protective equipment and clothing.

Disposal of Non-Recyclable Solvents: Dispose of in accordance
with all federal, state, and local health and pollution regulations.
Follow same guidelines as when disposing kerosene.

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52

Heat Transfer Fluid First Aid Procedures

Eye Contact: Immediately flush eyes with fresh water for at least 15
minutes. If irritation persists, get medical attention.

Skin Contact: Wash contaminated areas with soap and water.
Remove contaminated clothing and footwear. Wash clothing before
reuse. Discard footwear which cannot be decontaminated. Medical
attention may be required.

Inhalation: Remove patient to fresh air. If breathing stops, give
artificial respiration. Get medical attention immediately, if required.

Ingestion: Get medical attention immediately. Do not induce
vomiting.

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Original instructions

APPENDIX A

FREEZE DRYER COMPONENTS

The following pages list components that are available for your Freeze Dryer. The
parts shown are the most common replacement parts. If other parts are required,
contact Product Service.

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Appendix A: Freeze Dryer Components

54

Original instructions

1

4

5

8

11

12

10

3

13

2

6

14

15

Item

Part No.

Qty.

Description

1

7591806

2

Compressor 230V – 50 Hz (Not Shown)

7591807

Compressor 230V – 60 Hz (Not Shown)

2

7501902

1

Lexsol Pump 230V

3

7365401

1

Heater 230V

4

7211100

1

Door Gasket

5

7210900

1

Chamber Door Assembly (includes Gasket)

6

7365800

2

Temperature Sensor – Sample

7

7515302

1

Temperature Sensor – Collector (Not Shown)

8

7337600

1

Control Assembly

9

7840000

1 Gal.

Heat Transfer Fluid (Not Shown)

10

1338002

1

Power Cord 230V 60Hz US (Not Shown)

1332601

Power Cord 230V 60Hz Saudi (Not Shown)

1336500

Power Cord 230V 50Hz EU (Not Shown)

1332701

Power Cord 230V 50Hz China (Not Shown)

1332601

Power Cord 230V 50Hz UK (Not Shown)

1345701

Power Cord 230V 50Hz India (Not Shown)

11

7373436

1

Vacuum Hose

12

1488800

2

Hose Clamp

13

7343900

1

Bladder, Stoppering

14

7508600

4

Valve Knob

15

7505600

4

Valve Body

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Original instructions

Size

Labconco No (100 pcs.).

Shelf Capacity

2 ml

7575010

400

3 ml

7575210

441

5 ml

7573010

233

10 ml

7573210

196

20 ml

7573410

121

30 ml

7573610

86

50 ml

7573810

64

100 ml

7574010

42

125 ml

7574210

36

29.5

74.9cm

27.6

70.2cm

27.7

70.4cm

28.3

71.9cm

26.0
66cm

APPENDIX B

FREEZE DRYER DIMENSIONS

Serum Bottle Capacity of the Triad Freeze Dry System

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Original instructions

Voltage

Frequency

Amps

Catalog #

Description

Nominal

Operating

Range

(Hz)

Max. with

Vacuum Pump

794001010
794001015

Triad Freeze

Dryer

230V

187-253

60

12

794001030
794001040
794001050
794001070

Triad Freeze

Dryer

230V

198-253

50

12

APPENDIX C

FREEZE DRYER

SPECIFICATIONS

This Appendix contains technical information about the Freeze Dryer including
electrical specifications and operating conditions.

Electrical Specifications

Product Service 816-333-8811 or 1-800-522-7658

Appendix C: Freeze Dryer Specifications

57

Original instructions

Environmental Conditions

 Indoor use only.
 Ambient temperature range: 41° to 104°F (5° to 40°C).
 Maximum relative humidity: 80% for temperatures up to 88°F (31°C),

decreasing linearly to 50% relative humidity at 104°F (40°C).

 Main supply voltage fluctuations not to exceed ±10% of the nominal

voltage.

 Transient overvoltages according to Installation Categories II (Overvoltage

Categories per IEC 1010). Temporary voltage spikes on the AC input line
that may be as high as 1500V for 115V models and 2500V for 230V
models are allowed.

 Used in an environment of Pollution degrees 2 (i.e., where normally only

non-conductive atmospheres are present). Occasionally, however, a
temporary conductivity caused by condensation must be expected, in
accordance with IEC 664.

Product Service 816-333-8811 or 1-800-522-7658

58

Original instructions

APPENDIX D

FREEZE DRYER ACCESSORIES

Labconco offers a full line of accessories to enhance your Freeze Dryer’s operation

and usability. For a complete list of these accessories, please consult our website
at www.labconco.com.

Product Service 816-333-8811 or 1-800-522-7658