Labconco 7900000, 7900001, 7900002, 7900003, 7900010 User Manual

...
7900000
7900001
7900002
7900003
7900010
7900011
7900012
7900013
7910000
7910001
7910010
7910011
7910012
7910013
7910014
7910015
RapidVap Vacuum, N2 & N2/48
Models
Warranty
Labconco Corporation provides a warranty to the original buyer for the repair or replacement of parts and reasonable labor as a result of normal and proper use of the equipment with compatible chemicals. Broken glassware and maintenance items, such as filters, gaskets, light bulbs, finishes and lubrication are not warranted. Excluded from warranty are products with improper installation, erratic electrical or utility supply, unauthorized repair and products used with incompatible chemicals.
The warranty for RapidVap® Vacuum, N2 & N2/48 Evaporation Systems will expire one year from date of installation or two years from date of shipment from Labconco, whichever is sooner. Warranty is non­transferable and only applies to the owner (organization) of record.
Buyer is exclusively responsible for the set-up, installation, verification, decontamination or calibration of equipment. This limited warranty covers parts and labor, but not transportation and insurance charges. If the failure is determined to be covered under this warranty, the dealer or Labconco Corporation will authorize repair or replacement of all defective parts to restore the unit to operation. Repairs may be completed by 3rd party service agents approved by Labconco Corporation. Labconco Corporation reserves the rights to limit this
warranty based on a service agent’s travel, working hours, the site’s entry restrictions and unobstructed access
to serviceable components of the product.
Under no circumstances shall Labconco Corporation be liable for indirect, consequential, or special damages of any kind. This warranty is exclusive and in lieu of all other warranties whether oral, or implied.
Copyright © 2017 Labconco Corporation. The information contained in this manual and the accompanying products are copyrighted and all rights reserved by Labconco Corporation. Labconco Corporation reserves the right to make periodic design changes without obligation to notify any person or entity of such change.
Returned or Damaged Goods
Do not return goods without the prior authorization from Labconco. Unauthorized returns will not be accepted. If your shipment was damaged in transit, you must file a claim directly with the freight carrier. Labconco Corporation and its dealers are not responsible for shipping damages.
The United States Interstate Commerce Commission rules require that claims be filed with the delivery carrier within fifteen (15) days of delivery.
Limitation of Liability
The disposal and/or emission of substances used in connection with this equipment may be governed by various federal, state, or local regulations. All users of this equipment are required to become familiar with
any regulations that apply in the user’s area concerning the dumping of waste materials in or upon water,
land, or air and to comply with such regulations. Labconco Corporation is held harmless with respect to user’s compliance with such regulations.
Contacting Labconco Corporation
If you have questions that are not addressed in this manual, or if you need technical assistance, contact Labconco’s Customer Service Department or Labconco’s Product Service Department at 1-800-821-5525 or 1-816-333-8811, between the hours of 7:30 a.m. and 5:30 p.m., Central Standard Time.
Part #7490100, Rev. K
ECO #L122
ORIGINAL INSTRUCTIONS
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CHAPTER 1: INTRODUCTION 1
About This Manual 2 Typographical Conventions 3 Your Next Step 3
CHAPTER 2: PREREQUISITES 5
Electrical Requirements 5 Location and Exhaust Requirements 6 Vacuum Pump Requirements 6 Vacuum Line Traps 7 Nitrogen Supply Requirements 8 Space Requirements 8 Tools Required 8 Your Next Step 8
CHAPTER 3: GETTING STARTED 9
Unpacking Your RapidVap 9 RapidVap Components 10 Setting Up Your RapidVap 12
Vacuum Pump Connection 12 Sample Block 13 Nitrogen Gas Connection 15 Exhaust Port 15 Electrical Connection 15
Calibrate the Vacuum Sensor 15 Chemical Attack on RapidVap Components 15 Solvent Safety Precautions 17 Your Next Step 18
CHAPTER 4: USING YOUR RAPIDVAP 19
Planning 19 Glassware Selection and Speed Setting Guidelines 20 Loading Glassware Into the RapidVap 22 Heat Setting Guidelines 23 Time Setting Guidelines 27 RapidVap Controls 29
Operating the RapidVap Controls 30
Operating The RapidVap 32
Operational Notes 33 Interrupting a Cycle After it Has Begun 33 End Alarms 33 RS-232 Communications 34
RS-232 Programming Syntax 34
RS-232 Hardware Required 36
CHAPTER 5: MAINTAINING YOUR RAPIDVAP 37
CHAPTER 6: MODIFYING YOUR RAPIDVAP 39
Changing the Sample Blocks 39 Installing the Optional Lid Heater 40
CHAPTER 7: TROUBLESHOOTING 41
APPENDIX A: RAPIDVAP AND RAPIDVAP N2 COMPONENTS 45
APPENDIX B: RAPIDVAP DIMENSIONS 52
APPENDIX C: RAPIDVAP SPECIFICATIONS 54
Wiring Diagram (115V) 56 Wiring Diagram (230V) 57 Evaporation Rates 58
RapidVap Vacuum Models 58
RapidVap N2 Models 59
RapidVap N2/48 Models 63
APPENDIX D: RAPIDVAP ACCESSORIES 65
RapidVap Vacuum Model Accessories 65 RapidVap N2 Model Accessories 68 RapidVap N2/48 Model Accessories 70
1
Original instructions
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Congratulations on your purchase of a Labconco RapidVap. Labconco manufactures two types of RapidVaps - one utilizes vacuum and the other utilizes nitrogen as an aid to evaporation. Each model is available for operation on 115V or 230V.
Superior evaporation rates are achieved by the unique blending of several gentle forces on the sample. Samples are subjected to a gyrating motion causing the liquid to form a vortex configuration which greatly increases the surface area and hence the ability to evaporate. A precisely controlled amount of thermal energy can be supplied to the sample to heat it. In the vacuum model, the sample can be subjected to a decreased pressure by the application of a regulated vacuum. In summary, the vacuum model RapidVap performance is maximized by increasing the sample’s surface area, increasing temperature and decreasing pressure.
In the RapidVap N2, a stream of nitrogen or dry gas is directed downward onto the surface of the sample. This reduces the partial pressure directly over the liquid to speed evaporation and helps remove the solvent as it evaporates. In summary, the RapidVap N2 performance is maximized by increasing the sample’s surface area, increasing temperature and directing gas over the sample.
The gyrating motion imparted to the sample causes other, more subtle effects to occur. Excellent mixing of the components in the liquid sample is obtained. Also, as the liquid forms the vortex shape, a centrifugal force is established which forces the liquid outward against the tube walls. This helps prevent bumping and in turn allows the evaporation rate to be optimized.
The microprocessor which controls the operation of the heater and the motor provides excellent regulation and reproducibility of protocols. The end of the run can be signaled by the automatic end point system or by a manually preset timer. Nine different protocols can be stored in memory so exact test parameters can be easily repeated.
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Chapter 1: Introduction
2
Original instructions
About This Manual
This manual is designed to help you learn how to install, use, and maintain your RapidVap. Instructions for performing routine maintenance and making minor modifications to your RapidVap are also included.
Chapter 1: Introduction provides a brief overview of the RapidVap, explains the organization of the manual, and defines the typographical conventions used in the manual.
Chapter 2: Prerequisites explains what you need to do to prepare your site before you install your RapidVap. Electrical and exhaust requirements are discussed as well as vacuum pump or nitrogen requirements.
Chapter 3: Getting Started contains the information you need to properly unpack, inspect, install, and test your RapidVap.
Chapter 4: Using Your RapidVap discusses the basic operation of your RapidVap. Information on how to arrange the samples inside your RapidVap and select an operating cycle is included.
Chapter 5: Maintaining Your RapidVap explains how to perform routine maintenance on your RapidVap.
Chapter 6: Modifying Your RapidVap describes how to install other blocks and the optional lid heater.
Chapter 7: Troubleshooting contains a table of problems you may encounter while using your RapidVap, including the probable causes of the problems, and suggested corrective actions.
Appendix A: RapidVap and RapidVap N2 Components contains labeled diagrams of the components of the RapidVaps.
Appendix B: RapidVap Dimensions contains comprehensive diagrams showing the dimensions for the RapidVap and RapidVap N2.
Appendix C: RapidVap Specifications contains product specifications. Wiring diagrams for both the 115V and 230V RapidVaps are also included.
Appendix D: RapidVap Accessories lists the part numbers and descriptions of all of the accessories available for your RapidVap.
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Chapter 1: Introduction
3
Original instructions
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Typographical Conventions
Recognizing the following typographical conventions will help you understand and use this manual:
Book, chapter, and section titles are shown in italic type (e.g., Chapter 3:
Getting Started).
Steps required to perform a task are presented in a numbered format. Comments located in the margins provide suggestions, reminders, and
references.
Critical information is presented in boldface type in paragraphs that are
preceded by the exclamation icon. Failure to comply with the information following an exclamation icon may result in injury to the user or permanent damage to your RapidVap.
Les informations critiques sont présentées en caractères gras dans les
paragraphes qui sont précédés par le point d'exclamation. Le non respect de l'information suite à un point d'exclamation peut entraîner des blessures pour l'utilisateur ou des dommages permanents à votre RapidVap.
Important information is presented in capitalized type in paragraphs that are
preceded by the pointer icon. It is imperative that the information contained in these paragraphs be thoroughly read and understood by the user.
Information that is specific to a particular model of RapidVap is preceded by a
letter icon. The V icon indicates the text is specific to the Vacuum model. The N icon indicates the text is specific to the RapidVap N2 model.
CAUTION – See Manual. When this symbol is on the unit it indicates a
caution that is detailed in this manual.
ATTENTION – Voir le manuel. Lorsque ce symbole est allumé l'unité, il
indique une mise en garde qui est détaillée dans ce manuel.
Your Next Step
If your RapidVap needs to be installed, proceed to Chapter 2: Prerequisites to ensure your installation site meets all of the requirements. Then, go to Chapter 3: Getting Started for instructions on how to install your RapidVap and make all of the necessary connections.
For information on the operational characteristics of your RapidVap, go to Chapter
4: Using Your RapidVap.
If your RapidVap is installed and you need to perform routine maintenance on the RapidVap, proceed to Chapter 5: Maintaining Your RapidVap.
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Chapter 1: Introduction
4
Original instructions
For information on making modifications to the configuration of your RapidVap go to Chapter 6: Modifying Your RapidVap.
Refer to Chapter 7: Troubleshooting if you are experiencing problems with your RapidVap.
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5
Original instructions
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Before you install your RapidVap, you need to prepare your site for installation. Carefully examine the location where you intend to install your RapidVap. You must be certain that the area is level and of solid construction. In addition, an exhaust means must be provided and a source of nitrogen must be available for the RapidVap N2. An electrical source must be located near the installation site.
Carefully read this chapter to learn:
the electrical supply requirements. the exhaust requirements. the nitrogen supply requirements for the RapidVap N2.  the vacuum pump requirements for the RapidVap Vacuum.
Refer to Appendix C: RapidVap Specifications for complete RapidVap electrical and environmental conditions, specifications and requirements.
If the unit is not operated as specified in this manual it may impair the protection provided by the unit.
Si l'appareil n'est pas utilisé comme spécifié dans ce manuel, il peut compromettre la protection offerte par l'appareil.
Electrical Requirements
If your RapidVap is a Vacuum model, a dedicated electrical outlet is required. This outlet requires a 20 Amp circuit breaker or fuse for models rated at 115V (60 Hz). A 10 Amp circuit breaker or fuse is required for models rated at 230V (50/60 Hz). 115V models are equipped with a 20 Amp NEMA 5-20P plug. 230V models are equipped with a 16 Amp CEE 7/7 plug. It may be necessary to remove the plug and install a different plug to match the available receptacle.
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Chapter 2: Prerequisites
6
Original instructions
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If your RapidVap is a Nitrogen model, a dedicated electrical outlet is required. This outlet requires a 15 Amp circuit breaker or fuse for models rated at 115V (60 Hz) or an 8 Amp circuit breaker or fuse is required for 230V (50/60 Hz) models. 115V models are equipped with a 20 Amp NEMA 5-20P plug. 230V models are equipped with a 16 Amp CEE 7/7 plug. It may be necessary to remove the plug and install a different plug to match the available receptacle.
Do not use any detachable power cord that is not adequately rated for the unit.
N'utilisez pas de cordon d'alimentation amovible qui n'est pas suffisamment puissant pour l'unité.
Location and Exhaust Requirements
The RapidVap Vacuum Evaporation System should be located within a fume hood if hazardous or flammable solvents are used. In all cases, regardless of the solvent used, it is strongly recommended that the vacuum pump is located and vented in a fume hood.
The RapidVap N2 Evaporation System should be located within a fume hood if hazardous or flammable solvents are used. In all cases, regardless of the solvent used, it is recommended that the exhaust hose is vented into a fume hood or other laboratory ventilation device.
Do not position the unit so that it is difficult to operate the main disconnect device.
Ne pas placer l'appareil de sorte qu'il est difficile de faire fonctionner le dispositif de déconnexion principal.
Vacuum Pump Requirements
A vacuum pump must be provided by the user. For most applications, it is recommended that the vacuum pump is capable of a free air flow of 63 L/min and
1.5 mbar vacuum although, in some applications other pumps may be satisfactory or be required. The inlet fitting on the vacuum pump must be suitable for 0.50 I.D. hose.
Biological samples, for example, are often dissolved in water, a high boiling point solvent, and are often heat liable. Such samples require the high vacuum achievable with a mechanical vacuum pump, usually with an ultimate vacuum of 7 x 10-3 mBar to achieve a good evaporation rate.
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Chapter 2: Prerequisites
7
Original instructions
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On the other hand, samples dissolved in methylene chloride, a low boiling point solvent, can be efficiently concentrated using a water aspirator.
The nomograms in Chapter 4: Using Your RapidVap will help determine the boiling point of a solvent at pressures below atmospheric. This can serve as a guide to the appropriate vacuum source.
TO INSURE THAT AGGRESSIVE SAMPLES USED IN THE RAPIDVAP DO NOT DAMAGE THE VACUUM PUMP, IT IS RECOMMENDED THAT ALL INTERNAL WETTED PARTS ARE PTFE OR PTFE COATED. TO PREVENT A HAZARDOUS CONDITION, THE PUMP MUST BE SUITABLE FOR HANDLING THE VAPORS FROM THE SOLVENTS THAT WILL BE EVAPORATED IN THE RAPIDVAP.
CAUTION: When selecting the vacuum pump it is very important to consider the flammability of the solvents that will be used. If the solvents are flammable, an explosion proof vacuum pump is recommended.
ATTENTION: Lors de la sélection de la pompe à vide, il est très important d'examiner l'inflammabilité des solvants qui seront utilisés. Si les solvants sont inflammables, d'une pompe à vide à l'épreuve des explosions est recommandé
Vacuum Line Traps
When using a mechanical pump which is not corrosion resistant, a trap must be used in the vacuum line to prevent damage from solvent vapors. This is most often a condenser-type trap chilled with dry ice/acetone. When evaporating acids, it is advisable to include a soda lime acid trap in the vacuum line. This adds additional protection for the pump.
Several components within the RapidVap are made from stainless steel and can be degraded if exposed to acids. Before evaporating acids, be sure to contact Labconco.
Plusieurs composants du RapidVap sont en acier inoxydable et peuvent être dégradés s'ils sont exposés à des acides. Avant de s'évaporer acides, n'oubliez pas de contacter Labconco.
A moisture trap and radioisotope trap are also available. When evaporating high boiling point solvents, a liquid trap should be positioned between the RapidVap and the vacuum pump to prevent liquid from entering the pump. Refer to Appendix D: RapidVap Accessories for ordering information.
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Chapter 2: Prerequisites
8
Original instructions
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Nitrogen Supply Requirements
The 1/4" I.D. hose on the rear of the RapidVap N2 must be attached to a source of nitrogen or other suitable gas. Pressure must NOT exceed 20 psi. A regulator must be supplied by the user. The nitrogen supply must be capable of 0.6 CFM flow rate for the 8 place RapidVap N2. The RapidVap N2/48 requires a flow rate of 3.5 CFM. A suitable barb or compression fitting must be supplied by the user.
Space Requirements
Refer to Appendix C: RapidVap Specifications for dimensional drawings of the RapidVap.
Tools Required
Common hand tools are required to set up the RapidVap. A screwdriver or 5/16" socket is needed to attach hose clamps. A tubing cutter or knife is needed to shorten exhaust hoses. If the sample block must be installed or exchanged, attaching nuts on the RapidVap Vacuum and RapidVap N2 require a 7/16" socket. The manifold guides, which secure the block on the RapidVap N2/48, require a 9/16" socket.
Your Next Step
After you have determined that the location for your RapidVap accommodates the installation and operational requirements, you are ready to unpack and install your RapidVap. Proceed to Chapter 3: Getting Started.
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9
Original instructions
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The United States Interstate Commerce Commission rules require that claims be filed with the delivery carrier within fifteen (15) days of delivery.
Now that the site for your RapidVap is properly prepared, you are ready to unpack, inspect, install, and test your RapidVap. Read this chapter to learn how to:
unpack and move your RapidVap. set up your RapidVap. connect the vacuum pump or nitrogen source to your RapidVap. connect the electrical supply source to your RapidVap. properly exhaust your RapidVap. calibrate the vacuum sensor. solvent safety precautions.
Depending upon which model of RapidVap you are installing, you will need common hand tools including a flat blade screw driver, a 7/16" socket and an adjustable wrench to complete the instructions in this chapter.
The RapidVap weighs over 70 lbs. (33 Kg). The carton allows for lifting with a mechanical lift truck or hand truck. If you must lift the RapidVap manually, use at least two (2) persons and follow safe-lifting guidelines.
Unpacking Your RapidVap
Carefully unpack your RapidVap and inspect it for damage that may have occurred in transit. If your RapidVap is damaged, notify the delivery carrier immediately and retain the entire shipment intact for inspection by the carrier.
DO NOT RETURN GOODS WITHOUT THE PRIOR AUTHORIZATION OF LABCONCO. UNAUTHORIZED RETURNS WILL NOT BE ACCEPTED.
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Chapter 3: Getting Started
10
Original instructions
Catalog #
RapidVap Description
79000-00
RapidVap Vacuum System – 115V
79000-01
RapidVap Vacuum System – 230V
79000-02
RapidVap Vacuum System – 115V with lid heater
79000-03
RapidVap Vacuum System – 230V with lid heater
79000-10
RapidVap Vacuum System – 115V with RS 232 Interface
79000-11
RapidVap Vacuum System – 230V with RS 232 Interface
79000-12
RapidVap Vacuum System – 115V with lid heater with RS 232 Interface
79000-13
RapidVap Vacuum System – 230V with lid heater with RS 232 Interface
Plus the Following:
Part #
Component Description
78286-04
Hose - Vacuum
14888-00
Two (2) Clamps
13364-00
13365-00
Power Cord – 115V
or
Power Cord – 230V
19116-00
Washer (3)
19092-21
Acorn Nut (3)
12556-00
Heat Transfer Grease
74901-00
User’s Manual
74848-00
Cable Assembly RS232 (Models 79000-10, -11, -12, -13)
IF YOUR RAPIDVAP WAS DAMAGED IN TRANSIT, YOU MUST FILE A CLAIM DIRECTLY WITH THE FREIGHT CARRIER. LABCONCO CORPORATION AND ITS DEALERS ARE NOT RESPONSIBLE FOR SHIPPING DAMAGES.
Do not discard the carton or packing material for your RapidVap until you have checked all of the components and installed and tested the RapidVap.
RapidVap Components
As previously mentioned, Labconco manufactures two types of RapidVap, the Vacuum and the Nitrogen models. Each model is available in 115V or 230V.
The different RapidVap models require different assembly components. Locate the model of RapidVap you received in the following tables. Verify that the components listed are present and undamaged.
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Chapter 3: Getting Started
11
Original instructions
Catalog #
RapidVap Description
79100-00
RapidVap N2 Evaporation System – 115V
79100-01
RapidVap N2 Evaporation System – 230V
79100-10
RapidVap N2 Evaporation System – 115V with RS 232 Interface
79100-11
RapidVap N2 Evaporation System – 230V with RS 232 Interface
Plus the Following:
Part #
Component Description
79131-01
Exhaust Hose
19660-00
One (1) Clamp
79255-00
Eight (8) Sample Tube Caps
13364-00
13365-00
Power Cord – 115V
or
Power Cord – 230V
12556-00
Heat Transfer Grease
74901-00
User’s Manual
74848-00
Cable Assembly RS232 (Models 79100-10, -11)
Catalog #
RapidVap Description
79100-12
RapidVap N2/48 Evaporation System – 115V
79100-13
RapidVap N2/48 Evaporation System – 230V
79100-14
RapidVap N2/48 Evaporation System – 115V with RS 232 Interface
79100-15
RapidVap N2/48 Evaporation System – 230V with RS 232 Interface
Plus the Following:
Part #
Component Description
79131-00
Exhaust Hose – 6 ft.
19660-00
One (1) Clamp
13364-00
13365-00
Power Cord – 115V
or
Power Cord – 230V
12556-00
Heat Transfer Grease
74901-00
User’s Manual
74488-00
Manifold Guide (3)
19116-00
Washer (3)
74848-00
Cable Assembly RS232 (Models 74100-14, -15)
Since users’ preference of sample size varies, the sample holding block is not
included with the RapidVap Vacuum System or the RapidVap N2/48 Evaporation System. The correct size block must be selected and ordered separately. Refer to Appendix D: RapidVap Accessories for block selection.
If you do not receive one or more of the components listed for your RapidVap, or if any of the components are damaged, contact Labconco Corporation immediately for further instructions.
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Chapter 3: Getting Started
12
Original instructions
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Optional vacuum pump exhaust hose to ventilation device
Hose
Vacuum hose
Clamp all connections
Optional liquid trap or chilled trap or secondary trap
Vacuum pump inlet fitting
Vacuum pump power connection
Setting Up Your RapidVap
After you verify the RapidVap components, move your RapidVap to the location where you want to install it. Then, follow the steps listed below.
Vacuum Pump Connection
Connect the pump inlet to the fitting at the right hand rear corner of the RapidVap using the hose and clamps provided. Route the hose to prevent kinks. Clamp all connections securely. Plug the pump into the pump receptacle on the back of the unit and turn the pump switch on if it is so equipped. When evaporating high boiling point solvents, a liquid trap should be positioned between the RapidVap and the vacuum pump to prevent liquid from entering the pump. It is recommended that the vacuum pump is located inside a fume hood. If that is not possible, the exhaust of the pump should be directed to a laboratory fume hood or other laboratory ventilation device.
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Chapter 3: Getting Started
13
Original instructions
There is a switch on the back of the RapidVap Vacuum that is used to select the operation of an optional RapidVap Trapping Valve. When the RapidVap is used without the Trapping Valve as shown above, set the switch to the “Off” position.
Sample Block
If the RapidVap was not shipped with the sample block installed then it must be installed by the user. The sample block must be securely attached to its mounting plate. Note the asymmetric hole pattern of the three thru mounting holes. Locate the three flat washers either nuts or manifold guides that were shipped with the RapidVap. The RapidVap Vacuum uses nuts while the RapidVap N2/48 uses guide rods.
Apply a small amount of heat transfer grease that was shipped with the RapidVap to the end of the temperature sensor probe that extends up from the bottom of the chamber. Position the block over the three studs in the chamber. Install the flat washers and either nuts or guide rods. Tighten securely.
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Chapter 3: Getting Started
14
Original instructions
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Nut or guide rods – 3 required
Washer – 3 required
Block
Mounting stud
Temperature sensor
Apply heat transfer grease
Never lift the lid if the block is moving. Samples may splash out.
Ne jamais soulever le couvercle si le bloc est en mouvement. Les échantillons peuvent gicler.
Never operate the RapidVap without the sample block securely fastened in place. Excessive vibration will occur.
Ne jamais faire fonctionner le RapidVap sans le bloc d'échantillon fixé solidement en place. Des vibrations excessives se produira.
Never attempt to reach into the chamber if the block is moving.
Ne jamais tenter de mettre la main dans la chambre si le bloc est en mouvement.
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Chapter 3: Getting Started
15
Original instructions
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Nitrogen Gas Connection
Turn the gas supply off. Install a barb fitting suitable for a 1/4" I.D. hose into the “out” port of the regulator. Clamp securely. Alternately, a compression fitting may be used that is suitable for a hose with a 3/8" O.D. and 1/4" I.D.
THE NITROGEN PRESSURE MUST NEVER BE ALLOWED TO EXCEED 20 PSI. (103 KPA)
Exhaust Port
If the RapidVap N2 is not located in a fume hood, attach one end of the two inch diameter venting hose that is supplied with the RapidVap to the exhaust port on the right side of the unit. Clamp securely. Route the other end to a fume hood or other laboratory ventilation device.
Electrical Connection
Plug the power cord into the receptacle on the back of the RapidVap and plug the other end into a suitable wall power receptacle.
Calibrate the Vacuum Sensor
The vacuum sensor can be calibrated to room ambient atmospheric pressure. To do this, determine the atmospheric pressure using a known reference device such as a mercury barometer. Press and hold the vacuum release button on the RapidVap while turning on the RapidVap power switch. Use the increase or decrease buttons to make the vacuum display agree with the reference pressure. Press run to store the calibration in memory.
Chemical Attack on RapidVap Components
Your RapidVap Evaporation System is designed to be chemical resistant to most compounds that are commonly used in the concentration processes that are performed in it. However, by necessity, the RapidVap is comprised of a number of different materials, some of which may be attacked and degraded by certain chemicals. The degree of degradation is obviously dependent on the concentration and duration of exposure. Some of the major components of the RapidVap that are susceptible to degradation are as follows:
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Chapter 3: Getting Started
16
Original instructions
Acids
Bases
Solvents
COMPONENT
MATERIAL
Acetic Acid 20%
Boric Acid
Formic Acid Hydrobromic Acid 20%
Hydrochloric Acid 20%
Nitric Acid 20% Sulfuric Acid 10%
Trifluoroacetic Acid (TFA)
Ammonium Hydroxide
Potassium Hydroxide
Acetone Acetonitrile Chloroform
Dimethyl Formamide Dimethyl Sulfoxide (DMSO)
Ethanol Ethyl Acetate
Hexanes Isoproponal
Methylene
Chloride Toluene
Manifold N2/48
Acetal
C C C D D D C D C
Exhaust Elbow N2
Polyamid (Nylon)
D D D D D D D C D C
Blower Fan N2
Polyamid (Nylon)
D D D D D D D C D C
Exhaust Hose N2
Polyethylene
C C C C C C C
Vacuum Fittings
Polypropylene
C C
Blower Housing N2
Polypropylene
C C
Vacuum Hose
PVC D C D D D D D D C D D D D Trim Ring
Bromyl Butyl
C D D D D D D D
O- Rings
Neoprene
D D C D D C D D D D D D
Block Hardware
Stainless Steel
Nozzle N2
Stainless Steel
D D D
Vacuum Sensor
Stainless Steel
D D D
C- Moderate Degradation- Questionable Use D- Severe Degradation- Infrequent use recommended- immediate thorough cleaning required
If a rotary vane vacuum pump is used, most compounds used in the RapidVap
will degrade the oil if allowed to enter the pump. Generally, a rotary vane pump is not suitable for use with the RapidVap.
Diaphragm vacuum pumps sold by Labconco have wetted parts either made
from PTFE or protected by PTFE coatings and are suitable for nearly all procedures.
Glass lids are suitable for use with all common compounds.
When using compounds in the RapidVap that are hostile to the materials of construction, it is imperative that the equipment is properly maintained.
After each run, clean up all residues, spills, and materials that might have
splashed in the chamber using agents suitable for the substance involved.
When using a rotary vane pump the oil in the pump should be checked often. It
must be changed if it is cloudy, shows particles or is discolored. The useful life of vacuum pump oil can be extended if the vacuum pump is operated for an extended period of time after the RapidVap run is over. This allows contaminants to be purged from the hot oil. This must be done with the inlet to the pump blocked off to prevent air from free flowing through the pump. If the pump is operated at an elevated vacuum level, oil will be expelled from the pump and damage will occur.
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Original instructions
!
With prudent maintenance your RapidVap will provide years of service. Warranty on the effected parts will be void if maintenance has obviously been neglected. If you have questions about using specific compounds in your RapidVap, contact Labconco Technical Service at 1-800-821-5525 or 816-333-8811 or e-mail labconco@labconco.com.
Solvent Safety Precautions
The RapidVap is not classified as “explosion proof.” It has been designed with safety as a primary consideration and should be used in a prudent manner using
“good laboratory practices.” It has been designed for use with compounds as
described in the United States National Electrical Code Class I, Group D. The block that holds the sample tubes may be programmed to run as hot as 100°C, however, the heater element may normally run at 110°C. A thermal fuse limits the heater to a maximum temperature of 130°C. It is important that the solvents used are compatible with these temperatures. Do not evaporate solvents that have an autoignition temperature below 180°C. Do not evaporate solvents that are classified as Group A, B, or C by the National Electrical Code. Evaporate only non-flammable or Group D solvents with autoignition temperatures 180°C or above. Use of other compounds could cause an explosion.
Solvents used in the RapidVap may be flammable or hazardous. Use extreme caution and keep sources of ignition away from the solvents. When using flammable or hazardous solvents, both the RapidVap and the vacuum pump should be operated inside a fume hood.
If a sample is spilled in the chamber it must immediately be cleaned up.
Hazardous materials such as strong acids or bases, radioactive substances and volatile organics, must be handled carefully and promptly cleaned up if spilled.
Several components of the RapidVap which are located inside the chamber are made of stainless steel which can be attacked by acids. Use of acids such as trifluoroacetic acid can result in degradation of the product and the vacuum pump if the vacuum system is being used. Use care when using aggressive liquids which can damage the RapidVap and thoroughly clean the RapidVap after each use. Contact Labconco before evaporating acids.
WARNING: The disposal of substances used in connection with this equipment may be governed by various Federal, State or local regulations. All users of this equipment are urged to
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Original instructions
become familiar with any regulations that apply in the user’s
area concerning the dumping of waste materials in or upon water, land or air and to comply with such regulations.
AVERTISSEMENT: L'élimination des substances utilisées dans le cadre de ce matériel peut être régie par diverses réglementations fédérales, provinciales ou locales. Tous les utilisateurs de cet équipement sont invités à se familiariser avec tous les règlements qui s'appliquent dans le domaine de l'utilisateur concernant le déversement de déchets dans ou sur l'eau, la terre ou de l'air et de se conformer à ces règlements.
Your Next Step
The installation and setup of your RapidVap is now complete. To learn how to load and operate your RapidVap, proceed to Chapter 4: Using Your RapidVap. To make a modification to the configuration of your RapidVap, proceed to Chapter 6: Modifying your RapidVap. To perform additional diagnostics on your RapidVap, proceed to Chapter 7:Troubleshooting. To learn about the maintenance requirements for your RapidVap, proceed to Chapter 5: Maintaining Your RapidVap.
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19
Original instructions
C
C
HHAAPPTTEERR
4
4
U
U
SSIINNG
G
Y
Y
OOUURR
R
R
AAPPIID
D
V
V
AAPP
!
V
After your RapidVap has been installed as detailed in Chapter 3: Getting Started, you are ready to begin using your RapidVap. Read this chapter to learn how to:
set operating parameters. operate the controls. properly select and position glassware inside your RapidVap. understand the display. interrupt a cycle after it has begun.
Do not use the RapidVap in a manner not specified by the manufacturer (refer to Appendix C: RapidVap Specifications). The electrical protection properties of the RapidVap may be impaired if the RapidVap is used inappropriately.
Planning
Thoroughly understand procedures and the equipment operation prior to beginning work. The unique performance of the RapidVap Vacuum is dependent upon the proper balance of heat, vacuum and vortexing action. If the proper balance is not established, it is possible to damage or lose a portion of the sample. Therefore, if you are unfamiliar with the RapidVap or are attempting a new protocol, it may be helpful to make a trial run that is void of the sample you are attempting to concentrate.
Select the size of the sample tube so it is compatible with the block. Tubes should not be loose in the block. Blocks are available with holes for various size tubes. Refer to Appendix D: RapidVap Accessories for available block sizes and pages 19 and 20 for recommended usable tube volumes and vortex speeds.
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Original instructions
N
The unique performance of the RapidVap N2 is dependent upon the proper balance of heat, gas flow and vortexing action. If the proper balance is not established, it is possible to damage or lose a portion of the sample. Therefore, if you are unfamiliar with the RapidVap N2 or are attempting a new protocol, it may me helpful to make a trial run that is void of the sample you are attempting to concentrate.
Determine if the sample which is to be processed will be allowed to go to dryness or whether the evaporation is to cease prior to dryness. If the process should not go to dryness, the proper sample tubes must be selected to allow the desired amount of solvent to remain in the tubes. Sample tubes are available for various end point volumes. Refer to Appendix D: Accessories for available sample tubes. Any style ground glass tube may be used if the sample is to go to dryness, however, flat bottom tubes evaporate the quickest.
Glassware Selection and Speed Setting Guidelines
The speed at which the sample block should oscillate is dependent on the sample tube size and the sample volume. Generally, the tube should be no more than half full. The faster the speed, the higher the sample will vortex giving a maximum surface area and therefore a maximum evaporation rate. However, if the speed is too fast, samples will raise above the top of the tube and be lost. Smaller tubes can be run faster than larger tubes. The following table gives recommended safe speeds for tubes loaded with water. Experimentation with the specific sample may determine faster speeds. When setting the speed, keep in mind that the sample will raise up slightly in the tube when vacuum is applied to the chamber of the vacuum model.
GLASS VIALS USED IN THE RAPIDVAP SHOULD BE OF THE STYLE THAT DO NOT HAVE THE BEAD OR LIP ON THE TOP EDGE. IF VIALS WITH BEADS ARE USED, THE BEADS COULD HIT EACH OTHER AND BREAK THE GLASS.
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Original instructions
TUBE
SIZE
SAMPLE
VOLUME
VORTEX
SPEED
12 x 75 mm (6 ml)
3 ml
95%
13 x 100 mm (10 ml)
5 ml
95%
16 x 150 mm (23 ml)
12 ml
95%
15 ml centrifuge
7.5 ml
66%
28 mm (46 ml)
23 ml
48%
600 ml
50 ml
45%
100 ml
45%
200 ml
38%
300 ml
29%
400 ml
20%
450 ml
18%
TUBE
SIZE
SAMPLE
VOLUME
VORTEX
SPEED
170 ml
50 ml
50%
75 ml
47%
100 ml
42%
125 ml
35%
TUBE
SIZE
SAMPLE
VOLUME
VORTEX
SPEED
600 ml
50 ml
90%
100 ml
90%
200 ml
76%
300 ml
58%
400 ml
40%
450 ml
36%
170 ml
50 ml
100%
75 ml
95%
100 ml
85%
125 ml
70%
N
V
Several different size sample blocks are available for the RapidVap Vacuum model. The maximum recommended sample volumes and vortex speeds are as follows:
Two sample blocks are available for the RapidVap N2. Recommended speeds are as follows:
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Original instructions
TUBE
SIZE
SAMPLE
VOLUME
VORTEX
SPEED
12 mm (6 ml)
3 ml
100%
13 mm (10 ml)
5 ml
100%
16 mm (23 ml)
12 ml
100%
20 mm (35 ml)
18 ml
100%
N
Four sample blocks are available for the RapidVap N2/48. Recommended speeds are as follows:
Loading Glassware Into the RapidVap
Smooth operation of the RapidVap is dependent upon proper balance of the machine. Therefore, if less than a full load of samples is run, it is important to load samples into the RapidVap in a fairly uniform manner distributing the weight of the samples and glassware evenly in the sample block.
IN ORDER TO MAXIMIZE THE PERFORMANCE OF THE RAPIDVAP IT IS IMPORTANT THAT SOME OF THE SAMPLES ARE CLUSTERED AROUND THE FRONT LEFT HAND BLOCK MOUNTING STUD. THE BLOCK TEMPERATURE SENSOR IS LOCATED ADJACENT TO THIS BLOCK MOUNTING STUD. BY POSITIONING THE SAMPLE NEAR THE SENSOR, THE SENSOR WILL DETECT THE COOLING EFFECT OF THE EVAPORATING SAMPLES AND SIGNAL THE CONTROLS TO SUPPLY MORE HEAT TO THE SAMPLE BLOCK.
The best evaporation rate is achieved under conditions that maximize sample surface area. These include using the largest tube possible, and using the highest vortex speed possible.
When less than 8 samples are run in the RapidVap N2 or less than 48 samples are run in the RapidVap N2/48, nitrogen flow can be directed to 2, 4, or 6 positions. It is important to position glassware properly in the RapidVap N2 when less than a full block is run to ensure that nitrogen gas is directed downward where the sample is located. Note the label on the hub in the center of the chamber. This indicates which nozzles on a RapidVap N2 or nozzle clusters on a RapidVap N2/48 will be activated. If one or two positions are to be run, samples must be positioned at locations identified (2). If three or four positions are to be run, samples must be positioned at locations identified (4). If five or six positions are to be run, samples must be positioned at locations identified (6). If seven or eight positions are to be run, samples may be positioned at any of the locations. When using the RapidVap N2/48, glassware may be placed anywhere within the activated cluster of nozzles.
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Original instructions
V
Heat Setting Guidelines
The evaporation rate achieved by the RapidVap is dependent upon a variety of factors. These include the nature of the solvent, the temperature, the pressure in the vacuum system or the flow of the nitrogen gas in the RapidVap N2, the volume of sample, shape of tubes and vortex speed. Because of the multiplicity of factors it is impossible to give “typical” performance data.
By design, the RapidVap Vacuum model can be run at lower temperature settings than the RapidVap N2 model. This is because the decrease in pressure will cause the sample to evaporate at a lower temperature. As a general guideline, to speed the evaporation process, the RapidVap temperature should be set as high as possible as long as the temperature will not damage the sample or cause the sample to bump.
In the RapidVap Vacuum models, generally the evaporation rate will be optimized with the block temperature set and preheated 20 to 40°C above the boiling point of the solvent at the operating pressure.
For example, methanol (which boils at 65°C at atmospheric pressure) boils at 5°C at pressure of 53 mBar. Under these vacuum conditions, the block temperature should be set between 25 to 45°C. The nomogram and equation to follow will help in this determination.
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Original instructions
Calculating the boiling point:
The operating pressure corresponding to the desired boiling point temperature may be calculated for any solvent using the following formula:
log P = 2.8811 – Ta – T (b)(T)
Where P = Vacuum (mm Hg)
Ta = Boiling Point Temperature at Atmospheric Pressure (°K) T = Desired Boiling Point Temperature (°K) b = Constant from Table
Useful conversion factors: 1 mBar = .75 mm Hg
°K = °C + 273
Using the nomogram:
To use this graph, three facts must be known about a given solvent:
1. Boiling point at normal atmospheric pressure (1 Bar).
2. Constant b.
3. Either operating temperature or operating pressure.
To determine the desired boiling point at a given pressure:
1. Find the boiling point at normal atmospheric pressure (1 Bar) on the left side of
the graph.
2. Find the operating pressure on the right side of the graph.
3. Draw a straight line linking these two points.
4. Find “Constant b” for the solvent on the top of the grid. Follow that line until
it intersects with the line as drawn between the two points. This point represents the boiling point at the given pressure and the temperature is read off the left or right side of the grid (0-250°C).
To determine the operating pressure necessary to achieve a given boiling point:
1. Find the boiling point at normal atmospheric pressure (1 Bar) on the left side of
the graph.
2. Find the “Constant b” and follow this line to the desired boiling point on the
grid (0-250°C).
3. Draw a straight line linking these two points and extrapolate on the right hand
scale to find the operating pressure.
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Original instructions
SOLVENT
BOILING PT (°C)
DENSITY (g/cm³)
HEAT OF VAPORIZATION (Kcal/Kg)
CONSTANT b
VACUUM FOR BOILING POINT AT 40°C (MBAR)
Acetone
56
.790
125
0.196
556
Acetonitrile
82
.786
178
0.195
208
Acetylene tetrachloride
147
1.602
53
0.200
20
Benzene
80
0.879
93
0.202
236
Butyl alcohol
118
0.810
120
0.156
26
Carbon tetrachloride
77
1.594
45
0.206
271
Chlorobenzene
132
1.110
90
0.202
36
Chloroform
60
1.480
59
0.203
474
1.2 Dichloroethane
83
1.235
126
0.201
210
Dichloroethylene
55
1.250
41
0.200
584
Diproplyether
68
0.726
76
0.207
375
Dioxide
111
1.034
98
0.195
70
Ethyl acetate
77
0.900
102
0.189
240
Ethyl alcohol
78
0.791
205
0.159
175
Ethylene dichloride
85
1.256
78
0.200
194
Heptane
98
0.680
74
0.200
120
Hexane
69
0.659
85
0.206
360
Iso-amyl alcohol
132
0.800
120
0.160
15
Iso-butyl alcohol
108
0.803
120
0.160
44
Isopropyl alcohol
82
0.785
160
0.158
136
Methyl alcohol
65
0.792
267
0.167
337
Methylene chloride
40
1.325
41
0.194
1013
Methyl ethyl ketone
80
0.810
113
0.206
243
N-Amyl alcohol
137
0.814
142
0.158
11
Pentachloroethane
159
1.700
45
0.207
15
Pentane
36
0.626
85
0.214
1013
Propyl alcohol
97
0.804
165
0.154
67
Tert, Butanol
83
0.787
141
0.154
130
1, 1, 2, 2, Tetrachloroethane
146
1.603
60
0.198
20 Tetrachloroethylene
121
1.624
50
0.202
53
Toluene
111
0.867
86
0.202
76
1, 1, 1, Trichloroethane
74
1.346
76
0.206
301
Trichloroethylene
87
1.462
57
0.202
183
Water
100
1.000
540
0.167
72
Xylene
140
0.870
82
0.199
25
Physical constants:
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Original instructions
Determination of Operating Conditions Applied Vacuum/Boiling Point:
ts = Boiling point at 1 bar (normal pressure) tp = Boiling point at P mbar (operating pressure) p = Operating pressure in mbar
Example methyl alcohol:
1. Boiling point at normal (1 bar) atmospheric pressure = 65°C.
2. Constant b = 0.160
3. Operating pressure = 130 mBar
4. Boiling point at 130 mBar pressure = 24°C
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Original instructions
Constant b
Groups
0.200
Aliphatic hydrocarbons
Aliphatic halogenated hydrocarbons
0.195
Aromatic hydrocarbons
Aromatic halogenated hydrocarbons
Ketones
Ethers
Nitriles
Heterocyclic compounds
0.190
Aldehydes
Esters
0.185
Amines
0.180
Phenols
0.165
Acids
0.160
Alcohols
N
Time Setting Guidelines
It is often desirable to stop the concentration process before the solvent is entirely evaporated. This is accomplished by using glassware that has a small diameter
stem on the bottom and using the “Time” function to shut off the process after a
predetermined interval of time.
The “Cool Zone” feature is available on the RapidVap N2 with 8 places and is not available on the RapidVap N2 with 48 places.
The design of the RapidVap N2 causes the evaporation to proceed quickly when the sample is in the larger upper chamber of the sample tubes. When the solvent evaporates sufficiently to drop the level into the smaller stem, the evaporation rate decreases significantly. This phenomenon allows the solvent in all sample tubes to evaporate out of the large upper chamber before any of the small chambers are completely evaporated dry providing that all sample tubes are filled with nearly the same starting volumes.
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Original instructions
V
Cool-Zone
When it is necessary to end the concentration process with the sample still dissolved in a small amount of solvent, the time function should be activated and the process time set to allow the solvent sufficient time to evaporate completely out of the large upper portion of the sample tube and into the small lower portion
“Cool Zone.” This time can be approximated by knowing the starting sample size,
the desired end point volume, the stem size of the sample tube used and the solvent. The tables in Appendix C: RapidVap Specifications indicate the approximate time required to evaporate various common solvents. Actual times must be determined by the user. When the set time has expired, the RapidVap N2 will give an audible alarm and turn off all RapidVap functions.
The tables in Appendix C: RapidVap Specifications indicate approximate times required to evaporate various common solvents. Actual times must be determined by the user. The RapidVap can be set to alarm after a preset period of operation. When the time expires, the RapidVap will give an audible alarm and turn off all functions.
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Original instructions
1 2 4
6 7 3
5
1
6 5 4
3
2
RapidVap Controls
The control panel for the RapidVap vacuum model and the RapidVap N2 are shown below with a description about their function.
RapidVap Vacuum Model
RapidVap N2 Model
1. Display – The liquid crystal display (LCD) shows set point parameters and
actual measured conditions.
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Original instructions
2. Set Point Select Button – To select a parameter to change, press the set point
select button directly below the parameter.
3. Run/Stop Button – Used to start or stop a run.
4. Decrease Button – When pressed, the last selected set point will decrease.
5. Increase Button – When pressed, the last selected set point will increase.
6. Preheat Button – Used to turn on the heaters to preheat the sample block prior
to loading samples. When pressed during a run, it will switch the end point alarm from audible to muted or back to audible. The audible alarm is the default mode when the RapidVap is powered up.
7. Vacuum Release (Vacuum Model Only) – When pressed and held, the vacuum
valve will open to break vacuum. This is used to quickly decrease vacuum should bumping of the sample occur.
Operating The RapidVap Controls
Preheat:
To preheat the chamber, press the preheat button. The LED next to the button will illuminate and the display will show:
SP: XXC ACT: XXC
Press increase or decrease buttons until the desired set point is displayed. The actual block temperature will be displayed to the right. When power is being supplied to the heaters, a bar under the actual temperature will illuminate. The block will continue to preheat until either the preheat button is pressed again or the run button is pressed.
Select existing program:
Operating parameters can be stored in memory so protocols can be repeated. Nine programs can be stored. To select a program, press the set point select button
directly under the word “PROG.” An arrow will point to the current program
number indicating that this set point can be altered. To change the program number, press the increase or decrease button until the desired program number is displayed. When the program number is changed, all its set points will change also to indicate the last entered parameters. Pressing the “PROG” set point button will reset the timer to the previous set point time.
Change speed set point:
To change the speed set point parameter, press the set point select button directly under the word “SPEED.” Arrows will point to the present set point. The speed may be altered from 0 to 100% by pressing the increase or decrease buttons. The last entered set point will be stored in memory.
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Original instructions
V
N
It is suggested that the user set the vortex speed at a low percentage and gradually increase it after the run is started. This will minimize the possibility of samples sloshing out of the tubes. Reruns of the same protocol may start at the speed determined by previous runs.
Change heat set point:
To change the heat set point, press the set point select button under the word “HEAT.” Arrows will point to the present set point which can be changed from
-- (OFF) up to 99° by pressing the increase or decrease buttons. The set point is 100°C if the display shows “HI.” The last entered set point will be stored in memory.
Change time set point:
To change the time set point, press the set point select button under the word
“TIME.” Arrows will point to the present set point which can be changed from 1
to 999 minutes by pressing the increase or decrease buttons. If it is desired to have the RapidVap run continuously without alarming at the end of a time period, press
the increase button until the display says “RUN.” The last entered set point will be
stored in memory.
To reset the timer to the previous set point time, the “PROG” set point button must be pressed.
Change the Vacuum set point (Vacuum model only):
To change the vacuum set point, press the set point select button directly under the
word “VACUUM.” An arrow will point to the last entered vacuum set point. The
vacuum level may be set between 0 to 999 mBar by pressing the increase or decrease buttons. Above 999 mBar, the display will show “HI.” With a “HI” setting, the vacuum pump will not operate. The last entered set point will be stored in memory. Keep in mind that even though a deep vacuum set point may be entered, the actual vacuum level that the RapidVap will try to maintain can be no deeper than the vacuum level that the vacuum pump can maintain.
Select the number of samples that will be run (RapidVap N2 only):
Press the set point select button under the word “SAMPLE.” An arrow will point to the last entered set point. By pressing the increase or decrease button, the number of samples or clusters may be changed from 2 to 4 to 6 to 8. When running less than eight samples in the RapidVap N2 or 48 samples in the RapidVap N2/48, be sure to position them as described in the previous paragraph “Loading Glassware Into the RapidVap.”
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Original instructions
!
Operating The RapidVap
Never lift the lid if the block is moving. Samples may splash out.
Ne jamais soulever le couvercle si le bloc est en mouvement. Les échantillons peuvent éclabousser.
Never attempt to reach into the chamber if the block is moving.
Ne jamais tenter de mettre la main dans la chambre si le bloc est en mouvement.
Keep hands clear of lid at all times and do not place objects on the lid. Upon power up the unit may resume an interrupted program and the lid may start moving.
Gardez les mains éloignées de couvercle à tout moment et ne pas placer d'objets sur le couvercle. Dès la mise sous tension l'appareil peut reprendre un programme interrompu et le couvercle peut commencer à bouger.
1. Turn the power switch ON.
2. Select a program or set the set point parameters.
3. Turn on the nitrogen (RapidVap N2 only).
4. Preheat the block if desired.
5. Place samples in vials. Normally the vials should be no more than half full.
6. Load sample vials in the chamber.
7. Close the lid. On RapidVap N2/48 models, press down and rotate the cross bar
on the top of the lid counter clockwise approximately 1/8".
8. Swing both latches over the lid and tighten both knobs. Do not over tighten.
9. Press RUN. If the display was showing set point parameters, it will change to
show actual parameters. Press “RUN” again. The “S” (STOP) in the display
will change to “R” (RUN).
10. Set point parameters can be altered at any time during a run by first selecting
the parameter using the appropriate set point select button and then pressing the “Increase” or “Decrease” switch.
11. If the time set point is used, at the end of the set time an alarm will sound. All
operating functions will cease.
12. Press STOP to terminate operation if the RapidVap has not already stopped
itself.
13. When the evaporation is complete, allow the chamber to stop moving, unlatch
the latches, on the RapidVap N2/48 models rotate the cross bar clockwise approximately 1/8", lift the lid and remove the samples.
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Original instructions
14. Any time the RapidVap is not in operation, the power switch should be turned
OFF. This will prolong the life of the cooling fan and the blower in the RapidVap N2.
Operational Notes
The LCD display conveys various pieces of information. When the RapidVap is turned on, the RapidVap will return to the same mode (RUN or STOP) that it was in when the power was turned off. If the RapidVap was in the RUN mode when the power was turned off, when the power is turned on, the RapidVap will attempt to return to the programmed set points.
If the previous run is to be duplicated, it is important to press the program set point button prior to pressing run. This will show all the set points for that program and will reset the timer back to the programmed set point. Without pressing the program set point button, the time will not be reset and will continue to count down from the time at which it was stopped.
Interrupting a Cycle After it Has Begun
At any time during a run, the cycle may be stopped by pressing the STOP button. This shuts off all operating functions. After the chamber stops oscillating, the latches may be unlatched and the lid opened. If it is necessary to re-start the RapidVap, close the lid and secure the latches and press RUN. The RapidVap will resume operation at the same set point parameters and the timer will continue to count down from the time at which the RapidVap was stopped.
End Alarms
The RapidVap has two means to signal the completion of a run. The first method is determined by the operator when the run time is set as described in the previous paragraph, Time Setting Guidelines. In the second method the RapidVap will automatically sense when an evaporation process is nearly complete by comparing temperatures in the unit. When the RapidVap detects end point, it will sound an
alarm and the LED next to the “PREHEAT/ END ALARM” button will flash. This alerts the operator to closely monitor the run’s completion. This method performs
best when large samples are processed at high temperatures. When small samples or low temperatures are used, the alarm may signal prior to the end of evaporation.
The audible alarm may be canceled by pressing the “PREHEAT/ END ALARM”
button. The LED will continue to flash until the “STOP” button is pressed. If the button is pressed again, the audible alarm will become active again.
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Original instructions
#R[x];
x=0 for stop, x=1 for run and x=2 for pre-heat, will get a x’\n’
response for confirmation; when a “#R;” with no “[x]” is sent,
the RapidVap will respond 0, 1 or 2
#S[xxx];
Selects the vortex speed of program 9 from 0% or 12% to 100%, will get a xxx;yyy’\n’ response for confirmation. (xxx:speed setpoint, yyy:actual speed)
#T[xxx];
Selects the HEAT TEMPERATURE SET POINT of program 9 from 30 to 100 (C), will get a xxx;yyy’\n’ response for confirmation. (xxx:temperature setpoint, yyy:actual temperature); by entering “#T0;”, the heat will be set to OFF
RS-232 Communications
Some RapidVaps are equipped with RS-232 communications capabilities. This hardware and software allows a user to perform all the front panel functions from a standard RS-232 link. Communications will be at 4800 baud, no parity, 8 bit word, 1 stop bit, and a 3-wire interface (TXD, RXD and circuit common). The RS-232 link will be duplex or two-way, that is, it will allow the RapidVap Basic and N2 models to be controlled externally (incoming commands) as well as provide status information coming out of the RapidVap (outgoing status). All communications will be done in standard ASCII format using the programming syntax below.
RS-232 Programming Syntax
The RapidVap only responds to commands sent by the connected computer or terminal – it does not send out commands on its own. The programming syntax has two forms:
1. # [command abbreviation letter] [value]; when the command requires a
numeric value.
2. # [command abbreviation letter ]; when the command does not require a
numeric value.
When the programming syntax contains a numeric value, it will change the operation of the RapidVap. When the programming syntax does not contain a numeric value, the RapidVap will respond with its current status for that command value.
Note that the lead-in character is “#” while the “[ ]” (brackets) are shown only to separate parameters for ease of understanding. These brackets will not be used in
the actual ASCII command string. Finally, the “;” is used to indicate the end of a
command. Also note that ‘\n’ indicates a newline character.
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Chapter 4: Using Your RapidVap
35
Original instructions
#t[xxxx]
Selects the TIME SET POINT of program 9 from 1 to 999 minutes, and 1000 for no stopping (will run continuously), will get a xxxx;yyyy’\n’ response for confirmation. (xxxx:time setpoint, yyyy:time left)
#V[xxxx];
Selects the VACUUM SET POINT (if the RapidVap is a Vacuum model) of program 9 from 1 to 1000 mBar, will get a xxxx;yyyy’\n’ response for confirmation. (xxxx:vacuum setpoint, yyyy:actual vacuum)
#H[xxx];
Selects the PRE-HEAT SET POINT of program 9 from 30 to 100 C, will get a xxx;yyy’\n’ response for confirmation.(xxx:pre-heat setpoint, yyy:actual temperature)
#L[x];
KEY LOCKED when x=1 and UNLOCKED when x=0, will get a x’\n’ response for confirmation
#N[x]
elects the N2 VALVES (if the RapidVap is an N2 model) of program 9 from 0,2,4,6,8, will get a x’\n’ response for confirmation
#M[x];
x=1 will mute the audio alarm; x=0 will allow the audio alarm to work normally
#E;
The RapidVap will respond to this command with a numeric
value as follows :’”1"’=indicates an endpoint has been reached;
“0”=endpoint has not been reached
#e;
The RapidVap will respond to this command with a numeric
value as follows: 0=all functions are normal; “1”=lid is open; “2”=communication problem; “3” and later numbers will be
established later as specific error messages
Note: When controlling the RapidVap using the RS-232 link, all control parameters will be entered into program 9.
Note: All of the above commands can be sent with no numeric value, that is
‘#[command];’ (without value) to read out the setpoint and actual value without
changing the setpoint.
Note: There is no command for the vacuum release button on the front panel since this button is momentary in nature – the “#V0000;” command will bleed the vacuum chamber to atmospheric pressure.
Note: The above commands can be sent at a maximum rate of one command per second.
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Chapter 4: Using Your RapidVap
36
Original instructions
RS-232 Hardware Required
The connecting cable, Labconco #7484800 is supplied with the unit and is wired as shown below.
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37
Original instructions
C
C
HHAAPPTTEERR
5
5
M
M
AAIINNTTAAIINNIINNG
G
Y
Y
OOUURR
R
R
AAPPIID
D
V
V
AAPP
V
Under normal operation, the RapidVap requires little maintenance. The following maintenance schedule is recommended:
As needed:
1. Clean up all spills; remove liquids from chamber.
2. Clean lid and gasket using soft cloth, sponge or chamois and a mild, non-
Weekly:
1. If the vacuum pump contains oil, check the condition and level. If the oil level
Monthly:
1. The rubber components on the RapidVap may eventually deteriorate and
2. Using a soft cloth, sponge or chamois and a mild, non-abrasive soap or
abrasive soap or detergent.
is low, add oil. If the oil contains excessive amounts of moisture, detected by a cloudiness in the oil, it will be necessary to change the oil. For further information regarding procedures for changing the vacuum pump oil, refer to
the vacuum pump manufacturer’s instructions. To expel small amounts of
water that may have accumulated in the pump oil, periodically operate the vacuum pump against a dry, leak-tight system. The gas ballast must be open.
require replacement. The effective life of rubber parts depends upon both their usage and the surrounding environment. Check all rubber hoses and gaskets and replace any that show signs of hardening, permanent set or deterioration.
detergent, clean the glass lid.
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Chapter 5: Maintaining Your RapidVap
38
Original instructions
3. Using a soft cloth, sponge, or chamois and a mild, non-abrasive soap or
detergent, clean the exterior surfaces of the unit. Liquid spray cleaners and polishes may be used on the exterior surfaces. Do not use solvents to remove stains from the exterior surfaces as they may damage the finish.
4. All weekly activities.
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39
Original instructions
C
C
HHAAPPTTEERR
6
6
M
M
OODDIIFFYYIINNG
G
Y
Y
OOUURR
R
R
AAPPIID
D
V
V
AAPP
The configuration of your RapidVap may need to be changed to accommodate your needs. For example, you may want to change the sample size you are running so you will need to change the block or you may want to add a lid heater to eliminate condensation inside the lid. Read this chapter to learn how to:
change blocks. install a lid with heater.
Changing the Sample Blocks
If it is necessary to change from one sample size to another, it may be necessary to change the block. Refer to Appendix D: RapidVap Accessories for available sample blocks.
First, using a 7/16" socket (RapidVap Vacuum and RapidVap N2), remove the three nuts and washers on the top of the existing block. The RapidVap N2/48 requires a 9/16" socket to remove the three manifold guides that secure the block. Lift the block out of the chamber. Note the asymmetric hole pattern of the three mounting holes. Apply a small amount of heat transfer grease supplied with the RapidVap to the end of the temperature sensor probe that extends up from the bottom of the chamber.
Position the block over the three studs in the chamber. Install the flat washers and nuts or manifold guide rods. Tighten securely.
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Chapter 6: Modifying Your RapidVap
40
Original instructions
V
Installing the Optional Lid Heater
If a Lid Heater is to be added by the user, first disconnect the main power cord to the RapidVap. Then remove the existing lid by removing the two screws that hold either the RH or LH hinge pivot support bracket. Remove the bracket and then slide the lid assembly off of the other bracket. Install the new lid with the heater attached by reversing the process. Plug the jack attached to the lid heater into the receptacle on the back of the RapidVap. The lid heater will operate any time the main power switch is on.
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41
Original instructions
C
C
HHAAPPTTEERR
7
7
T
T
RROOUUBBLLEESSHHOOOOTTIINNG
G
DISPLAY ERROR CODE
CAUSE
CORRECTIVE ACTION
Top Temp Sensor
Top sensor failure
Replace top sensor assembly.
Connection failure
Repair connection.
Bottom Temp Sensor
Bottom sensor failure
Replace bottom sensor assembly. Connection failure
Repair connection.
Vacuum Sensor
Sensor failure
Replace sensor assembly.
Connection failure
Repair connection.
Lid Opened
Lid open
Close lid.
Switch failure
Replace switch.
Connection failure
Repair connection.
Refer to the following if your RapidVap fails to operate properly. If the suggested corrective actions do not solve your problem, contact Labconco for additional assistance. The following failure codes may appear on the display when problems are sensed by the internal self check routine.
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Chapter 7: Troubleshooting
42
Original instructions
DISPLAY ERROR CODE
CAUSE
CORRECTIVE ACTION
Mem Fail – Push Prog
Memory failure
Push program button.
New memory IC chip
Push program button.
Bad memory IC chip
Call Labconco – Replace IC chip or control PCB.
Motor Error
Stalled condition
Turn power off. Try to restart after one minute. Call Labconco if error message remains.
Excessive speed detected, i.e. >120%
Turn power off. Try to restart after one minute. Call Labconco if error message remains.
Defective motor
Turn power off. Try to restart after one minute. Call Labconco if error message remains.
PROBLEM
CAUSE
CORRECTIVE ACTION
Unit will not operate
Unit not connected to electrical power
Connect unit to proper electrical receptacle.
Circuit breaker blown
Correct electrical problem and reset circuit breaker by pressing button.
Lid open
Close lid.
Excessive vibration
Sample tubes not located symmetrically in block
Reposition sample tubes.
Sample block loose
Tighten all three mounting screws.
Sample block not installed
Install sample block.
Sample odor in lab
Vent hose exhausting into lab area
Redirect hose to fume hood.
Inoperable blower (RapidVap N2)
Replace blower.
Other corrective actions for potential problems are as follows:
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Chapter 7: Troubleshooting
43
Original instructions
PROBLEM
CAUSE
CORRECTIVE ACTION
Sample in bottom of chamber
Gas pressure too high (RapidVap N2)
Reduce pressure.
Vortex speed too high
Reduce speed.
Condensation forming inside lid (RapidVap Vacuum)
Install lid heater. See page 36.
Poor recovery rates
Sample going to dryness (RapidVap N2)
Program RapidVap N2 to prevent dryness.
Sample sloshing out of tube
Decrease vortex speed. Heat setting is too high
Decrease heat.
Evaporation rate is reduced
Gas supply depleted (RapidVap N2)
Replenish gas. Heater inoperable
Contact Labconco.
Vacuum pump failure (RapidVap Vacuum)
Check pump.
No vacuum poor vacuum (RapidVap Vacuum)
Pump not on
Turn on pump.
Control valve open
Check control valve.
Leaks in lines or connectors
Locate and repair.
Foreign material on lid gasket
Clean gasket and lid. Vacuum pump oil low
Add oil.
Excessive contamination in vacuum pump oil
Replace oil. Pump not connected to RapidVap
Connect hose and power cord.
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Chapter 7: Troubleshooting
44
Original instructions
PROBLEM
CAUSE
CORRECTIVE ACTION
No gas flow (RapidVap N2)
Gas depleted
Install new supply of gas.
Sample position not activated
Activate position per page 30. Nozzle clogged
Unplug nozzle with fine wire.
Valve inoperable
Contact Labconco
Gas flows continuously
Valve inoperable
Contact Labconco Control inoperable
Contact Labconco
Product Service 1-800-522-7658
45
Original instructions
A
A
PPPPEENNDDIIXX
A
A
R
R
AAPPIID
D
V
V
AAPP
V
V
AACCUUUUMM AANNDD
R
R
AAPPIID
D
V
V
AAPP
N
N
2
2
C
C
OOMMPPOONNEENNTTSS
The following pages list components that are available for your RapidVap. The parts shown are the most common replacement parts. If other parts are required, contact Product Service.
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Appendix A: RapidVap Vacuum & RapidVap N2 Components
46
Original instructions
Item
Quantity
Part No.
Description
1 1 13023-00
Switch, Power
2 4 74953-00
Foot 3 1
79167-00
Fan Cooling
4 1 74838-00
PCB, Main (RapidVap Vacuum)
4A 1 74838-03
PCB, Main (RapidVap N2 & RapidVap N2/48)
4B 1 74838-04
PCB, Main (RapidVap Vacuum w/RS232)
4C 1 74838-05
PCB, Main (RapidVap N2 & RapidVap N2/48 w/ RS232)
5 8 74887-00
Isolator, Upper
6 8 74915-00
Isolator, Lower
7 1 13364-00
Cord, Power (115V)
7A 1 13365-00
Cord, Power (230V)
8 1 12926-00
Triac 9 1
79176-00
Diode Bridge
10 1 78252-00
Belt
11 1 12111-00
Motor
-- 1 13444-06
Fuse, 12A, Type T, Slow Blow (Not Shown)
RapidVap Vacuum and RapidVap N2 Components
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Appendix A: RapidVap Vacuum & RapidVap N2 Components
47
Original instructions
1
2 3 4 5 6 7 8 9 11
10
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Appendix A: RapidVap Vacuum & RapidVap N2 Components
48
Original instructions
Item
Quantity
Part No.
Description
21 1 74917-00
Temperature Sensor, Lower
22 1 74870-00
Temperature Sensor, Upper
23 1 74916-00
Vacuum Sensor
24 2 74882-00
Spring, Latch
25 2 74880-00
Latch
26 6 19271-05
Washer, Latch
27 2 74883-00
Knob, Latch
28 3 19092-21
Nut, Block
29 3 19116-00
Washer, Block
30 3 79046-01
Stud, Block
31 1 74979-00
Trim Ring
32 1 15532-00
Hose, Exhaust
33 1 74847-00
Shield, Lid
34 1 74846-00
Lid
34A 1 74844-00
Lid With Heater
35 1 79759-01
Thermal Fuse
36 1 73449-00
Valve, Vacuum
37 1 74920-02
PCB, Display/Control
38 1 74926-00
Label, Control Panel
39 1 13061-00
Relay, Vacuum Pump
--
1
Fuse, Main Vacuum PCB, 2A, Type T, Slow Blow (Not Shown)
RapidVap Vacuum Components
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Appendix A: RapidVap Vacuum & RapidVap N2 Components
49
Original instructions
26
34
33
32
31
30
35
28
29
27
25
24
23
22
21
39
36
37
38
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Appendix A: RapidVap Vacuum & RapidVap N2 Components
50
Original instructions
Item
Quantity
Part No.
Description
51 1 74917-00
Temperature Sensor, Lower
52 1 74870-00
Temperature Sensor, Upper
53 2 74882-00
Spring, Latch
54 2 74880-00
Latch
55 6 19271-05
Washer, Latch
56 2 74883-00
Knob, Latch
57 3 19092-21
Nut, Block (RapidVap N2)
57A 3 74488-00
Guide Stud (RapidVap N2/48)
58 3 19116-00
Washer, Block
59 3 79046-01
Stud, Block
60 1 74845-00
Lid (RapidVap N2)
60A 1 74497-00
Lid (RapidVap N2/48)
61 1 74979-00
Trim Ring
62 1 79131-01
Hose, Exhaust Short
63 1 74889-01
Valve Assembly, Nitrogen
64 1 79131-02
Hose Exhaust Long
65 1 79096-00
Blower, Exhaust
66 1 74920-02
PCB, Display/Control
67 1 74922-00
Label, Control Panel
68 1 79759-01
Thermal Fuse
-- 1 13444-03
Fuse, Exhaust Blower, 4A, Type T, Slow Blow (Not Shown)
RapidVap N2 and RapidVap N2/48 Components
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Appendix A: RapidVap Vacuum & RapidVap N2 Components
51
Original instructions
51
67
66
65
64
63
62
68
61
58
60A
59
53
56
54
52
57A
59
58
57
55
Product Service 1-800-522-7658
52
Original instructions
A
A
PPPPEENNDDIIXX
B
B
R
R
AAPPIID
D
V
V
AAPP
D
D
IIMMEENNSSIIOONNSS
RapidVap Vacuum
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Appendix A: RapidVap Vacuum & RapidVap N2 Components
53
Original instructions
RapidVap N2
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54
Original instructions
A
A
PPPPEENNDDIIXX
C
C
R
R
AAPPIID
D
V
V
AAPP
S
S
PPEECCIIFFIICCAATTIIOONNSS
This Appendix contains technical information about the RapidVap including specifications, environmental operations conditions, wiring diagrams and evaporation rates.
Electrical Specifications
Nominal amperage for 115V RapidVap Vacuum (model 79000-00 &
Nominal amperage for 230V RapidVap Vacuum (model 79000-01 &
Nominal amperage for 115V RapidVap Vacuum (model 79000-02 &
Nominal amperage for 230V RapidVap Vacuum (model 79000-03 &
Nominal amperage for 115V RapidVap N2 (model 79100-00, 79100-10,  Nominal amperage for 230V RapidVap N2 (model 79100-01, 79100-11,  Frequency (all models): 50/60 Hz
Phase: Single Heater power: 1000 watts Lid heater power: 40 watts
Environmental Conditions
Indoor use only. Maximum altitude: 6562 feet (2000 meters). Ambient temperature range: 41° to 104°F (5° to 40°C). Maximum relative humidity: 80% for temperatures up to 88°F (31°C),
Main supply voltage fluctuations not to exceed ±10% of the nominal
79000-10) excluding vacuum pumps: 8A Maximum amperage with vacuum pump: 16A
79000-11) excluding vacuum pumps: 4A Maximum amperage with vacuum pump: 8A
79000-12) with Lid Heater excluding vacuum pump: 8.6A Maximum amperage with vacuum pump: 16A
79000-13) with Lid Heater excluding vacuum pump: 4.3A Maximum amperage with vacuum pump: 8A
79100-12 & 79100-14): 8A
79100-13 & 79100-15): 4A
decreasing linearly to 50% relative humidity at 104°F (40°C).
voltage.
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Appendix C: RapidVap Specifications
55
Original instructions
N
Transient overvoltages according to Installation Categories II (Overvoltage
Categories per IEC 1010). Temporary voltage spikes on the AC input line that may be as high as 1500V for 115V models and 2500V for 230V models are allowed.
Used in an environment of Pollution degrees 2 (i.e., where normally only
non-conductive atmospheres are present). Occasionally, however, a temporary conductivity caused by condensation must be expected, in accordance with IEC 664.
Nitrogen Consumption
Gas flow RapidVap N2 with 8 active nozzles:
0.55 SCFM @ 12 psi
0.41 SCFM @ 6 psi
Gas flow RapidVap N2/48with 48 active nozzles:
3.5 SCFM @ 20 psi
2.5 SCFM @ 10 psi
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Appendix C: RapidVap Specifications
56
Original instructions
Wiring Diagram (115V Models)
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Appendix C: RapidVap Specifications
57
Original instructions
Wiring Diagram (230V Models)
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Appendix C: RapidVap Specifications
58
Original instructions
Solvent:
Methylene
Chloride
Toluene
Acetonitrile
Water
Block Preheat & Block Set Point Temperature:
50°C
80°C
75°C
80°C
Vacuum:
250 mbar
133 mbar
167 mbar
133 mbar
Starting Volume
300ml
62
90
102
402
250
56
72
84
345
200
49
66
71
289
150
42
57
60
239
100
36
33
49
189
50
22
40
37
95
V
Evaporation Rates
RapidVap Vacuum Models
The following tables show the approximate time to evaporate various solvents in 75mm and 16mm sample tubes when the sample block is fully loaded. Other sample blocks are available for different size tubes. If they are used or if a different number of samples is run at one time, the evaporation times can be estimated from these charts or determined experimentally.
Evaporation Time – 75 mm dia. Sample Tube
(Time in Minutes)
Number of samples: 8 Vortex speed: 28% Vacuum pump: Labconco model – 79249-00 Rated free air flow – 88 L/min Max. attainable vacuum – 31 mbar
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Appendix C: RapidVap Specifications
59
Original instructions
Solvent:
Methylene
Chloride
Toluene
Acetonitrile
Water
Block Preheat & Block Set Point Temperature:
50°C
80°C
75°C
80°C
Vacuum:
200 mbar
200 mbar
330 mbar
133 mbar
Starting Volume
10 ml
18
25
25
119 8 14
20
22
101 6 10
14
17
72
4
8
12
13
50
2
4 6 7
26
V
N
Evaporation Time – 16 mm dia. Sample Tube
(Time in Minutes)
Number of samples: 69 Vortex speed: 95% Vacuum pump: Labconco model – 79249-00 Rated free air flow – 88 L/min Max. attainable vacuum – 31 mbar
RapidVap N2 Models
The following tables indicate the approximate time required to evaporate various common solvents. Tables A and B show the average time required to evaporate 8 samples from the selected beginning volume to a level where the solvent is at the top of the Cool-Zone stem. Tables C shows the average time to evaporate various solvents in different length Cool-Zone stems. It shows the average time for solvents to evaporate from the top of the stem to the indicated remaining volume when 8 vials are run simultaneously. The following example illustrates the use of the charts:
Assume that a starting volume of 300 ml methylene chloride is placed in a 600 ml sample vial with a 1.5 ml stem. The vial is covered with a cap. The desired endpoint volume is 0.5 ml. From Table A, the time to evaporate to the top of the stem is 59 minutes. From Table C, the time to evaporate from the top of the stem to 0.5 ml is 17 minutes. Adding the two times together, the total time to evaporate 300 ml of methylene chloride to 0.5 ml in a sample tube with a 1.5 ml stem is approximately 76 minutes.
If fewer than eight samples are run at a time, the time to evaporate will become shorter.
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Appendix C: RapidVap Specifications
60
Original instructions
Solvent:
Methylene
Chloride
Ethyl
Acetate
Hexanes
Acetone
Toluene
Water
Block Preheat & Block Set Point Temperature:
40°C
75°C
70°C
55°C
100°C
95°C
Starting Volume
450 ml
91
82
54
90
109
623
400
80
65
52
75
80
531
350
69
56
35
64
69
437
300
59
48
29
54
60
379
250
51
40
22
45
50
323
200
43
33
19
38
41
270
150
36
26
16
31
32
221
100
28
19
11
23
23
173
50
18
12 7 14
13
106
Table A
Evaporation Time in Upper Portion of Sample Vial
(600 ml Sample Tubes) (Time in Minutes)
N
Number of samples: 8 Nitrogen pressure: 10 psi at volumes 0 thru 300 ml (see Note 1) 5 psi at volumes above 300 thru 350 ml 4 psi at volumes above 350 thru 400 ml 2 psi at volumes above 400 thru 450 ml Vortex speed: 70% at volumes 0 thru 300 ml (see Note 1) 36% at volumes above 300 thru 450 ml Sample tubes covered with caps
Note 1: Even though the RapidVap N2 is capable of processing starting volumes as high as 450 ml using the 600 ml sample vial, its optimum volume is 300 ml. Above 300 ml, the vortex speed must be reduced and the nitrogen pressure must be decreased to prevent the sample from splashing out. This data for starting volumes over 300 ml was generated by starting the evaporation at reduced levels of nitrogen pressure and vortex speed. As the solvent evaporated, the nitrogen pressure and vortex speed were increased.
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Appendix C: RapidVap Specifications
61
Original instructions
Solvent:
Methylene
Chloride
Ethyl
Acetate
Hexanes
Acetone
Toluene
Water
Block Preheat & Block Set Point Temperature:
40°C
75°C
70°C
55°C
100°C
95°C
Starting Volume
450 ml
58
56
33
68
75
420
400
53
48
29
61
63
352
350
48
40
24
54
52
321
300
41
35
21
47
45
266
250
35
31
19
43
40
230
200
30
26
16
38
35
193
150
25
22
14
34
28
153
100
19
17
11
29
22
120
50
10 9 7
16
15
68
Table B
Evaporation Time in Upper Portion of Sample Vial
(600 ml Sample Tubes) (Time in Minutes)
N
Number of samples: 8 Nitrogen pressure: 10 psi at volumes 0 thru 300 ml (see Note 1) 5 psi at volumes above 300 thru 350 ml 4 psi at volumes above 350 thru 400 ml 2 psi at volumes above 400 thru 450 ml Vortex speed: 70% at volumes 0 thru 300 ml (see Note 1) 36% at volumes above 300 thru 450 ml Sample tubes: Not covered with caps
Product Service 1-800-522-7658
Appendix C: RapidVap Specifications
62
Original instructions
Solvent:
Methylene
Chloride
Ethyl
Acetate
Hexanes
Acetone
Toluene
Water
Block Preheat & Block Set Point Temp:
40°C
75°C
70°C
55°C
100°C
95°C
Glassware
Ending
Volume
0.5 ml Stem
0.5
Dry
4
10 4 9 3 7 4 9 5 10
42 78
1.5 ml Stem
1.5
1.0
0.5
Dry
4
9 17 31
4
7 13 24
2
5
8 16
3
7 12 22
5
9 15 26
23 43 75
120
2.0 ml Stem
2.0
1.0
Dry
3 17 64
3 11 34
2
8 26
3 15 47
4 14 50
28 80
201
3.0 ml Stem
3.0
2.0
1.0
Dry
4 20 57
128
4 12 34 81
3 10 25 52
3 16 50
108
5 17 40 89
34 76
170 339
Table C
Evaporation Rate in Cool-Zone Stem
(600 ml Sample Tubes) (Time in Minutes)
Number of samples: 8 Vortex speed: 70% Nitrogen pressure: 10 psi Sample tubes: Covered or not covered with caps
Glassware Used:
0.5 ml (PN 79259-00) .77 in (1.9 cm) Long Stem
1.5 ml (PN 79139-00) 1.37 in (3.5 cm) Long Stem
2.0 ml (PN 79137-00) 1.62 in (4.1 cm) Long Stem
3.0 ml (PN 79135-00) 2.25 in (5.7 cm) Long Stem
N
Product Service 1-800-522-7658
Appendix C: RapidVap Specifications
63
Original instructions
Solvent:
Methylene
Chloride
Toluene
Acetonitrile
Methanol
Hexane/
Ethyl Acetate
75:25
Hexane/
Ethyl Acetate
50:50
Block Preheat & Block Set Point Temp:
40°C
80°C
75°C
40°C
40°C
40°C
Starting Volume
2 ml
6 8 8
18 5 5
Evaporation Time RapidVap N2/48 Models
(12 mm Sample Tubes) (Time in Minutes)
Number of samples: 48 Vortex speed: 100% Nitrogen pressure: 8 psi
N
RapidVap N2/48 Models
The following tables show the approximate time to evaporate various solvents in 12mm and 20mm sample tubes when the sample block is fully loaded. Other sample blocks are available for different size tubes. If they are used or if a different number of samples is run at one time, the evaporation times can be estimated from these charts or determined experimentally. Evaporation rates may vary from tube to tube.
Product Service 1-800-522-7658
Appendix C: RapidVap Specifications
64
Original instructions
Solvent:
Methylene
Chloride
Toluene
Acetonitrile
Methanol
Hexane/
Ethyl Acetate
75:25
Hexane/
Ethyl Acetate
50:50
Block Preheat & Block Set Point Temp:
50°C
80°C
75°C
40°C
40°C
40°C
Starting Volume
10 ml
17
23
24
49
18
20
8
14
21
21 6
11
18
18 4
9
15
14 2
5 9 8
Evaporation Time RapidVap N2/48 Models
(20 ml Sample Tubes) (Time in Minutes)
Number of samples: 48 Vortex speed: 100% Nitrogen pressure: 20 psi
Product Service 1-800-522-7658
65
Original instructions
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V
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PART #
DESCRIPTION
7491300
Block. 110-12 mm dia. tubes.
7485800
Block. 110-13 mm dia. tubes.
7491400
Block. 69-16 mm dia. tubes.
7496300
Block. 69-15 ml centrifuge tubes.
7496400
Block. 26-28 mm scintillation tubes.
7494500
Block. 8-600 ml tubes. Order tubes from Labconco.
7486400
Block. 8-170 ml tubes. Order tubes from Labconco.
Block. Special orders (size and number of tubes). Contact Labconco.
7909200
Sample Tube Flat Bottom. Borosilicate glass. 600 ml capacity. Package of one (1).
7913408
Sample Tube Flat Bottom. Borosilicate glass. 600 ml capacity. Package of eight (8).
V
This following accessories are available for the RapidVap Vacuum model.
Product Service 1-800-522-7658
Appendix D: RapidVap Accessories
66
Original instructions
PART #
DESCRIPTION
7927000
Sample Tube Flat Bottom. Borosilicate glass. 170 ml capacity. Package of one (1).
7927108
Sample Tube Flat Bottom. Borosilicate glass. 170 ml capacity. Package of eight (8).
7393000
Vacuum Pump. Diaphragm pump - corrosion resistant. 115V, 60 Hz,
4.0 amps, single phase. 63 liters per minute, 1.5 mbar max vacuum.
7393001
Vacuum Pump. Diaphragm pump - corrosion resistant. 230V, 50/60 Hz, 3.0 amps, single phase. 63 liters per minute, 1.5 mbar max vacuum.
7587000
Vacuum Pump. Scroll pump. 115 VAC, 50/60 Hz, 10 amps, single phase. 189/195 liters per minute, 7 x 10-3 mbar max vacuum. Requires ½ fitting 1472701.
7587002
Vacuum Pump. Scroll pump. 230 VAC, 50/60 Hz, 6 amps, single phase. 189/195 liters per minute, 7 x 10-3 mbar max vacuum. Requires ½ fitting 1472701.
7587300
Silencer. For scroll pumps 7587000 and 7587002.
1472700
Fitting. For scroll pumps 7587000 and 7587002. For ½ I.D. hose.
7815300
Secondary Trap Canister. Includes stand and fittings. Requires an insert suitable for the application.
7814800
Acid Trap Insert. Installs inside secondary trap canister. Contains acid neutralizing desiccant.
Product Service 1-800-522-7658
Appendix D: RapidVap Accessories
67
Original instructions
PART #
DESCRIPTION
7814900
Moisture Trap Insert. Installs inside secondary trap canister. Contains desiccant.
7815000
Radioisotope Trap Insert. Installs inside secondary trap canister. Contains activated charcoal.
7815200
Solvent Trap Insert. Installs inside secondary trap canister. Contains molecular sieve.
7538000
Secondary Vacuum Trap. 9 ¾" h x 7 7/8" diameter with ¾" vacuum connections. Volume of inner pot holds 2.85 liters of dry ice and alcohol. Ice trapping capacity is over 900 ml. Shipping weight 9 lbs.
7538200
Secondary Vacuum Trap. 9 ¾" h x 7 7/8" diameter with 1/2" vacuum connections. Volume of inner pot holds 2.85 liters of dry ice and alcohol. Ice trapping capacity is over 900 ml. Shipping weight 9 lbs.
7538400
Secondary Vacuum Trap. 7 7/8" h x 6 5/8" diameter with 1/2" vacuum connections. Volume of inner pot holds 1.8 liters of dry ice and alcohol. Ice trapping capacity is 200 ml. Shipping weight 5 lbs.
7486500
Lid Heater. Includes glass lid with heater attached.
7873400
Liquid Trap. Capable of trapping 2,550 ml.
7970500
Liquid Trap. Capable of trapping 240 ml.
7348000
Trapping Valve. Requires a user supplied trap.
Product Service 1-800-522-7658
Appendix D: RapidVap Accessories
68
Original instructions
PART #
DESCRIPTION
7486400
Block. 8–170 ml tubes. Order tubes from Labconco.
7927000
Sample Tube Flat Bottom. Borosilicate glass. 170 ml capacity. Package of one (1).
7927108
Sample Tube Flat Bottom. Borosilicate glass. 170 ml capacity. Package of eight (8).
7926600
Sample Tube End Point Stem 1.5 ml. Borosilicate glass. 170 ml capacity. Package of one (1).
7926908
Sample Tube End Point Stem 1.5 ml. Borosilicate glass. 170 ml capacity. Package of eight (8).
7909200
Sample Tube Flat Bottom. Borosilicate glass. 600 ml capacity. Package of one (1).
7913408
Sample Tube Flat Bottom. Borosilicate glass. 600 ml capacity. Package of eight (8).
7925900
Sample Tube End Point Volume 0.5 ml. Borosilicate glass. 600 ml capacity. Package of one (1).
7926008
Sample Tube End Point Volume 0.5 ml. Borosilicate glass. 600 ml capacity. Package of eight (8).
7913900
Sample Tube End Point Volume 1.5 ml. Borosilicate glass. 600 ml capacity. Package of one (1).
7914008
Sample Tube End Point Volume 1.5 ml. Borosilicate glass. 600 ml capacity. Package of eight (8).
N
The following accessories are available for the RapidVap N2.
Product Service 1-800-522-7658
Appendix D: RapidVap Accessories
69
Original instructions
PART #
DESCRIPTION
7913700
Sample Tube End Point Volume 2 ml. Borosilicate glass. 600 ml capacity. Package of one (1).
7913808
Sample Tube End Point Volume 2 ml. Borosilicate glass. 600 ml capacity. Package of eight (8).
7913500
Sample Tube End Point Volume 3 ml. Borosilicate glass. 600 ml capacity. Package of one (1).
7913608
Sample Tube End Point Volume 3 ml. Borosilicate glass. 600 ml capacity. Package of eight (8).
7487600
Rack For 600 ml Sample Tubes. Holds eight (8) tubes.
7486300
Rack For 170 ml Sample Tubes. Holds eight (8) tubes.
7925408
Sample Tube CapPTFE For 600 ml Sample Tubes. Package of eight (8).
7926808
Sample Tube CapPTFE For 170 ml Sample Tubes. Package of eight (8).
7926508
Sample Tube CapPolyethylene For 170 ml Sample Tubes. Package of eight (8).
7925500
Sample Tube Cap–Polyethylene – For 600 ml Sample Tubes.
Package of one (1).
Product Service 1-800-522-7658
Appendix D: RapidVap Accessories
70
Original instructions
PART #
DESCRIPTION
7482100
Block. 48 – 12 x 75 mm tubes.
7482200
Block. 48 – 13 x 100 mm tubes.
7482300
Block. 48 – 16 x 150 mm tubes.
7482400
Block. 48 – 20 x 150 mm tubes.
7494500
Block. 8 – 600 ml glass tubes.
7909200
Glass Tubes for Block 74945-00. 600 ml flat bottom. Pkg. of 1.
7913408
Glass Tubes for Block 74945-00. 600 ml flat bottom. Pkg. of 8.
N
The following accessories are available for the RapidVap N2/48.
Product Service 1-800-522-7658
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