Labconco 7810017, 7810012, 7810011, 7810010, 7810015 Instruction Manual

...

CentriVap Centrifugal Concentrators

and Cold Traps

Models

78100 Series 78110 Series 74600 Series 73850 Series 79820 Series 79830 Series 79840 Series 74750 Series

Warranty

Labconco Corporation provides a warranty to the original buyer for the repair or replacement of parts and reasonable labor as a result of normal and proper use of the equipment with compatible chemicals. Broken glassware and maintenance items, such as filters, gaskets, light bulbs, finishes and lubrication are not warranted. Excluded from warranty are products with improper installation, erratic electrical or utility supply, unauthorized repair and products used with incompatible chemicals.

The warranty for CentriVap® Centrifugal Concentrators and Cold Traps will expire one year from date of installation or two years from date of shipment from Labconco, whichever is sooner. Warranty is nontransferable and only applies to the owner (organization) of record.

Buyer is exclusively responsible for the set-up, installation, verification, decontamination or calibration of equipment. This limited warranty covers parts and labor, but not transportation and insurance charges. If the failure is determined to be covered under this warranty, the dealer or Labconco Corporation will authorize repair or replacement of all defective parts to restore the unit to operation. Repairs may be completed by 3rd party service agents approved by Labconco Corporation. Labconco Corporation reserves the rights to limit this warranty based on a service agent’s travel, working hours, the site’s entry restrictions and unobstructed acce ss to serviceable components of the product.

Under no circumstances shall Labconco Corporation be liable for indirect, consequential, or special damages of any kind. This warranty is exclusive and in lieu of all other warranties whether oral, or implied.

products are copyrighted and all rights reserved by Labconco Corporation. Labconco Corporation reserves the right to make periodic design changes without obligation to notify any person or entity of such change.

Returned or Damaged Goods

Do not return goods without the prior authorization from Labconco. Unauthorized returns will not be accepted. If your shipment was damaged in transit, you must file a claim directly with the freight carrier. Labconco Corporation and its dealers are not responsible for shipping damages.

The United States Interstate Commerce Commission rules require that claims be filed with the delivery carrier within fifteen (15) days of delivery.

Limitation of Liability

The disposal and/or emission of substances used in connection with this equipment may be governed by various federal, state, or local regulations. All users of this equipment are required to become familiar with any regulations that apply in the user’s area concerning the dumping of waste materials in or upon water, land, or air and to comply with such regulations. Labconco Corporation is held harmless with respect to user’s compliance with such regulations.

Contacting Labconco Corporation

If you have questions that are not addressed in this manual, or if you need technical assistance, contact Labconco’s Customer Service Department or Labconco’s Product Service Department at 1-800-821-5525 or 1-816-333-8811, between the hours of 7:30 a.m. and 5:30 p.m., Central Standard Time.

Part #7539800, Rev. I

ECO L447

AABBLLEE

OONNTTEENNTTSS

CHAPTER 1: INTRODUCTION 1

Safety Symbols 2

CHAPTER 2: PREREQUISITES 3

Electrical Requirements 4 Location and Exhaust Requirements 4 Vacuum Pump Requirements 5 Vacuum Line Traps 5 Space Requirements 5

CHAPTER 3: GETTING STARTED 6

Unpacking Your CentriVap 7 CentriVap Components 7 Emergency Access Into the Chamber 10 Component Orientation & Hose Connections 10 Electrical Connection 12 Rotor Installation 12 Ground Wire 12 Chemical Resistance of CentriVap Components 12 Solvent Safety Precautions 14

CHAPTER 4: USING YOUR CENTRIVAP 16

Planning 16 Glassware Selection 16 Loading Glassware Into the CentriVap 17 Temperature Setting Guidelines 17 Time Setting Guidelines 17 Heat Boost Operation 17 CentriVap Controls 18 Operating the CentriVap Controls 18 Operating The CentriVap 20 Operational Notes 21 Cycle Interruption 21 Safety Precautions 21

CHAPTER 5: MAINTAINING YOUR CENTRIVAP 22

CHAPTER 6: ACCESSORIES FOR YOUR CENTRIVAP 24

Installing a Glass Lid 27 Installing a Secondary Chemical Trap 27 Installing a Vacuum Gauge 28 Installing a CentriZap Strobe Light 29 Installing a Glass Trap in the Cold Trap 30

CHAPTER 7: TROUBLESHOOTING 31

APPENDIX A: CENTRIVAP COMPONENTS 34

APPENDIX B: CENTRIVAP DIMENSIONS 42

APPENDIX C: CENTRIVAP SPECIFICATIONS 46

Electrical Specifications 46 Environmental Conditions 47 Evaporation Rates 48

CChhaapptteerr 11:: IInnttrroodduuccttiioonn

Congratulations on your purchase of a Labconco CentriVap Concentrator System. Models are available for operation on 115V or 230V.

The CentriVap Concentrator, when combined with the CentriVap Cold Trap, uses centrifugal force with heat and vacuum to rapidly evaporate and condense solvents from biological and analytical samples. Centrifugation eliminates bumping and foaming as vacuum is applied and also concentrates the solute in the bottom of the vial. This allows recovery of solutes from sample volumes as small as a few microliters. The concentrator is equipped with a 300 watt heater to speed evaporation by warming the chamber during processing. A timed outlet for the vacuum pump connection delays the start of the pump until the rotor reaches operating speed. A safety switch prevents the concentrator from starting when the lid is in the open position and a latch prevents the lid from being opened while the rotor is turning. The compact, low profile design allows the concentrator to be placed on a benchtop or other laboratory work surface.

The microprocessor, which controls the operation of the heater and the motor, provides excellent regulation and reproducibility of protocols. The end of the run can be signaled by a manually preset timer. Nine different protocols can be stored in memory so exact test parameters can be easily repeated.

As the companion to the Concentrator, the CentriVap Cold Trap protects the vacuum pump by trapping moisture, vapors and corrosive fumes as they evaporate from the samples. The stainless steel trap is used for aqueous and organic applications. For corrosive applications, the optional Glass Trap insert should be used. The 230V 50Hz models comply with CE regulations.

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Chapter 1: Introduction

Safety Symbols

Your CentriVap Concentrator was designed with safety in mind, however conditions may exist that could be hazardous.

Throughout this manual potentially hazardous conditions are identified using the following words and symbols.

It is important that you understand the warnings listed throughout this manual before you operate the CentriVap Concentrator.

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CChhaapptteerr 22:: PPrreerreeqquuiissiitteess

Before you install your CentriVap, you need to prepare your site for installation. You must be certain that the area is level and of solid construction. In addition, a means to exhaust the vacuum pump must be provided. An electrical source must be located near the installation site.

Carefully read this chapter to learn:

 The electrical supply requirements.  The exhaust requirements.  The vacuum pump requirements.

Refer to Appendix C: CentriVap Specifications for complete electrical and environmental conditions, specifications and requirements.

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Chapter 2: Prerequisites

Electrical Requirements

The CentriVap Concentrator requires a dedicated grounded electrical outlet. This outlet requires a 15 Amp circuit breaker or fuse for models rated at 115V (60 Hz). An 8 Amp circuit breaker or fuse is required for models rated at 230V (50/60 Hz). If the power cord supplied with the Concentrator does not match the available receptacle, replace it with an approved power cord of the suitable style. See CentriVap Components in Chapter 3 for power cord specifications.

The CentriVap Cold Trap requires a dedicated electrical outlet. 115V (-50°C &

-85°C) Models require a 15 Amp circuit breaker or fuse rated at 115V (60 Hz). 115V (-105°C) Models require a 20 Amp circuit breaker or fuse rated at 115V (60 Hz). 230V Models require an 8 Amp circuit breaker or fuse rated at 230V (50/60 Hz). If the power cord supplied with the Cold Trap does not match the available receptacle, replace it with an approved power cord of the suitable style. See CentriVap Components in Chapter 3 for power cord specifications.

Location and Exhaust Requirements

The CentriVap Concentrator should be located on a surface that is stable, flat and level.

WARNING: The CentriVap System should be located within a fume hood if hazardous or flammable solvents are used. Heating of materials could lead to the liberation of hazardous gases. In all cases, regardless of the solvent used, it is strongly recommended that the vacuum pump is vented in a fume hood. An accessory secondary trap is available to minimize the exhausting of solvents into the atmosphere. This does not, however, negate the need to exhaust the vacuum pump into a fume hood. Failure to properly vent the CentriVap will expose personnel to potentially harmful chemicals.

The CentriVap has not been evaluated by an approval agency for the use of biological, radio toxins or flammable liquids or materials.

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Chapter 2: Prerequisites

Vacuum Pump Requirements

A vacuum pump must be provided by the user. A vacuum pump with a free air flow rate of 82 liters per minute and < 2 mBar ultimate pressure is adequate for aqueous samples. More volatile samples can be satisfactorily processed using a diaphragm pump with a free airflow of 42 liters per minute and 200 mBar vacuum. The inlet fitting on the vacuum pump must be suitable for 0.50 ID hose.

Vacuum pumps used with 115V models should be equipped with a 115V, 15 Amp NEMA 5-15P plug. Vacuum pumps used with 230V models should be equipped

with a “reverse” IEC 320 plug. This will allow the vacuum pump to be plugged

into the receptacle on the back of the CentriVap.

IMPORTANT NOTE: To ensure that aggressive samples used in the CentriVap do not damage the vacuum pump, it is recommended that all internal wetted parts are PTFE or PTFE coated.

When selecting the vacuum pump it is very important to consider the flammability of the solvent that will be used. If the solvents are flammable, an explosion-proof vacuum pump or one suitable for the solvents to be processed is recommended. See Chapter 3 Solvent Safety Precautions for solvents suitable for use in the CentriVap.

Vacuum Line Traps

When using a mechanical pump that is not corrosion resistant, the CentriVap Cold Trap or equivalent trap must be used in the vacuum line to prevent damage from solvent vapors. When evaporating acids, it is advisable to include a soda lime acid secondary trap in the vacuum line. This adds additional protection for the pump.

NOTE: Several components within the CentriVap are made from stainless steel or aluminum and can be degraded if exposed to acids. Contact Labconco before evaporating acids.

A moisture trap, solvent trap and radioisotope trap are also available. Refer to Chapter 6: Accessories for Your CentriVap for ordering information.

Space Requirements

Refer to Appendix C: CentriVap Specifications for dimensional drawings of the CentriVap.

No person or any hazardous material should be within 12 inches of the CentriVap Concentrator while it is operating.

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CChhaapptteerr 33:: GGeettttiinngg SSttaarrtteedd

Now that the site for your CentriVap is properly prepared, you are ready to unpack, inspect, install, and test your CentriVap. Read this chapter to learn how to:

 Unpack and move your CentriVap.  Set up your CentriVap.  Connect the electrical supply source to your CentriVap.  Properly exhaust your CentriVap.  Safely use solvents with your CentriVap.

CAUTION: The CentriVap Concentrator weighs over 42 lbs. (19 Kg). The carton allows for lifting with a mechanical lift truck or hand truck. If you must lift the CentriVap manually, use at least two (2) persons and follow safe lifting guidelines.

The CentriVap Cold Trap weighs over 70 lbs. (33 Kg). The carton allows for lifting with a mechanical lift truck or hand truck. If you must lift the Cold Trap manually, use at least two (2) persons and follow safe lifting guidelines.

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Chapter 3: Getting Started

1334500

1336100

1332600

1332700

1338000

1336400

1336500

1332601

1332701

1342100

7828606

1488800

7464600

7539800

Catalog #

Temperature

Rating

Voltage (V)

Frequency (Hz)

Connector: IEC C13

Plug: NEMA 5-15

Connector: IEC C13

Plug: CEE 7/7

Connector: IEC C13

Plug: BS 1363

Connector: IEC C13

Plug: CH1-10P

Connector: IEC C13

Plug: NEMA 6-15

Connector: IEC C19

Plug: NEMA 5-20

Connector: IEC C19

Plug: CEE 7/7

Connector: IEC C19

Plug: BS 1363

Connector: IEC C19

Plug: CH2-16P

Connector: IEC C19

Plug: NEMA 6-20

Tubing

clamps (2)

Wire Assembly

User's Manual

7811020 -50˚C 115 60 X X X X X 7811030 -50˚C 230 50 X X X X X 7811035 -50˚C 230 50 X X X X X 7811037 -50˚C 230 50 X X X X X 7811040 -50˚C 230 60 X X X X X 7460020 -85˚C 115 60 X X X X X 7460030 -85˚C 230 50 X X X X X 7460035 -85˚C 230 50 X X X X X 7460037 -85˚C 230 50 X X X X X 7460040 -85˚C 230 60 X X X X X 7385020 -105˚C 115 60 X X X X X 7385030 -105˚C 230 50 X X X X X 7385035 -105˚C 230 50 X X X X X 7385037 -105˚C 230 50 X X X X X 7385040 -105˚C 230 60 X X X X X

Description

Power Cords

Accessories

Cold Traps

Unpacking Your CentriVap

Carefully unpack your CentriVap and inspect it for damage that may have occurred in transit. If your CentriVap is damaged, notify the delivery carrier immediately and retain the entire shipment intact for inspection by the carrier.

The United States Interstate Commerce Commission rules require that claims be filed with the delivery carrier within fifteen (15) days of delivery.

Do not discard the carton or packing material for your CentriVap until you have checked all of the components and installed and tested the CentriVap.

NOTE: Do not return goods without the prior authorization of Labconco. Unauthorized returns will not be accepted. If your CentriVap was damaged in transit, you must file a claim directly with the freight carrier. Labconco Corporation and its dealers are not responsible for shipping damage.

CentriVap Components

Locate the model of CentriVap you received in the following tables. Verify that the components listed are present.

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Chapter 3: Getting Started

1334500

1336100

1332600

1332700

1338000

7828606

1488800

7397601

7396206

7539800

Catalog #

Options

Voltage (V)

Frequency (Hz)

Connector: IEC C13

Plug: NEMA 5-15

Connector: IEC C13

Plug: CEE 7/7

Connector: IEC C13

Plug: BS 1363

Connector: IEC C13

Plug: CH1-10P

Connector: IEC C13

Plug: NEMA 6-15

Tubing

clamps (2)

Coupling Insert (Std.)

Coupling Insert (AR)

User's Manual

7810010 standard 115 60 X X X X X 7810011 standard 230 60 X X X X X 7810012 heat boost 115 60 X X X X X 7810013 heat boost 230 60 X X X X X 7810014 glass lid 115 60 X X X X X 7810015 glass lid 230 60 X X X X X 7810016 acid resistant 115 60 X X X X X 7810017 acid resistant 230 60 X X X X X 7810030 standard 230 50 X X X X X 7810031 heat boost 230 50 X X X X X 7810032 glass lid 230 50 X X X X X 7810033 acid resistant 230 50 X X X X X 7810034 standard 230 50 X X X X X 7810035 heat boost 230 50 X X X X X 7810036 glass lid 230 50 X X X X X 7810037 acid resistant 230 50 X X X X X 7810038 standard 230 50 X X X X X 7810039 heat boost 230 50 X X X X X 7810040 glass lid 230 50 X X X X X

7810041 acid resistant 230 50 X X X X X

Concentrators

Description

Power Cords

Accessories

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7460900 7814900 7814800 7815200

7455100

7455101 7397605

1553800 7828606 7474001 1488800 1486000

Catalog #

System Type

Voltage (V)

Frequency (Hz)

7810010 7810011 7810014 7810015 7810016 7810017 7810030 7810032 7810033 7810034 7810036 7810037 7810038 7810040 7810041 7811020 7811030 7811035 7811037 7811040

4330100 4330150

Clear Cannister

Moisture Insert

Acid Insert

Solvent Insert

Rotor Assembly

12-13mm

Rotor Assembly

12-13mm (Teflon)

Glass Trap

Tubing 1/4" ID

Tubing 1/2" ID

Valve Assembly

Clamps (2) .56 - 1.25"

Clamps (2) .48 - .78"

7982010 Aqueous 115 60 X X X X X

7982011 Aqueous 230 50 X X X X X

7982012 Aqueous 230 60 X X X X X

7982035 Aqueous 230 50 X X X X X

7982037 Aqueous 230 50 X X X X X

7983013 Acid 115 60 X X X X X X

7983014 Acid 230 50 X X X X X X

7983015 Acid 230 60 X X X X X X

7983035 Acid 230 50 X X X X X X

7983037 Acid 230 50 X X X X X X

7984010 Solvent 115 60 X X X X X

7984011 Solvent 230 50 X X X X X

7984012 Solvent 230 60 X X X X X

7984035 Solvent 230 50 X X X X X

7984037 Solvent 230 50 X X X X X

7475010 Gel Drying 115 60 X X X X X X X X X

7475011 Gel Drying 230 50 X X X X X X X X X

7475012 Gel Drying 230 60 X X X X X X X X X

Concentrator Systems

Description

Concentrators

Cold Traps

Gel Dryers

Accessories

Chapter 3: Getting Started

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Chapter 3: Getting Started

If you do not receive one or more of the components listed for your CentriVap, contact Labconco Corporation immediately for further instructions.

Emergency Access into the Chamber

The CentriVap is designed to prevent access to the chamber in the event of a power disruption. If it is necessary to open the lid when there is no electrical power connected to the CentriVap, insert a small screwdriver or similar instrument into the small round hole on the left side of the case near the top behind the control panel. This will unlock the lid latch mechanism. While holding the screwdriver in place, raise the lid with the other hand.

CAUTION: Never attempt to defeat the latch or open the lid while the CentriVap is running. Personnel injury can result from moving parts and chemicals.

Component Orientation & Hose Connections

The relative position of the CentriVap Concentrator, Cold Trap and the vacuum pump may be as shown or may be reversed. The refrigeration system in the Cold Trap draws air in through the right side of the cabinet and exhausts air through the left side of the cabinet. A minimum of 3" should be allowed between the sides of the Cold Trap and the adjacent wall surfaces. Restriction of the airflow through the cabinet during operation could adversely affect performance.

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Chapter 3: Getting Started

After positioning the components, it is necessary to join the system together using the hoses provided. Attach one hose to the tube that extends out the back of the Concentrator. Attach the other end of this hose to one of the barb fittings on the Cold Trap Cover Assembly. Secure the hoses with the clamps supplied. Attach another hose to the remaining barb fitting on the Cold Trap Cover and clamp securely. If the accessory Secondary Trap is not used, attach the other end of the hose to the inlet port on the vacuum pump. If the accessory Secondary Trap is used, install the Secondary Trap as explained in Chapter 6: Accessories for Your

CentriVap. Then attach the hose from the Cold Trap Cover to the “out” connector

on the Secondary Trap and clamp securely. Attach another hose from the remaining connector on the Secondary Trap to the inlet port on the vacuum pump and clamp securely.

WARNING: It is recommended that the vacuum pump be located inside a fume hood or other laboratory ventilation device if hazardous solvents are used in the CentriVap. If this is not possible, the vacuum pump should have a hose attached to the exhaust port and the other end of the hose should be positioned inside the fume hood or ventilation device.

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Chapter 3: Getting Started

Electrical Connection

Plug the power cord into the receptacle on the back of the CentriVap Concentrator and plug the other end into a suitable power receptacle. Plug the power cord into the receptacle on the back of the CentriVap Cold Trap and plug the other end into a suitable power receptacle. Plug the power cord from the vacuum pump into the receptacle on the back of the Concentrator. If the vacuum pump has an off/on switch, turn the switch ON. The vacuum pump will be controlled by the Concentrator.

Rotor Installation

Place the rotor onto the shaft of the Concentrator. Rotate the rotor slightly to engage the drive pin in the shaft with the slots in the rotor hub. The top of the shaft should be in line with the top of the rotor hub.

INPORTANT NOTE: Do not use a rotor if it shows any signs of damage. Failure of a spinning rotor could damage the CentriVap or cause samples to be lost.

Ground Wire

Attach one end of the included ground wire to the stainless steel elbow on the drain tubing on the right or left side (depending on model) of the Cold Trap.

CAUTION: When draining the CentriVap Cold Trap always attach the other end of the grounding clip to the solvent catch pot to eliminate the risk of electrostatic spark ignition.

Chemical Resistance of CentriVap Components

Your CentriVap Centrifugal Concentrator and Cold Trap are designed to be chemical resistant to most compounds that are commonly used in concentration processes. However, by necessity, the CentriVap is comprised of a number of different materials, some of which may be attacked and degraded by corrosive chemicals. The degree of degradation is obviously dependent on the concentration and duration of exposure. Some major components of the CentriVap that are susceptible to degradation are as follows:

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