permanent display: deceleration profile last set 1- 9
Speed / RCF
run: current value of speed or RCF after activation of
switch key
end: "End"
lid open: "OPEN"
"Lift Lid" (if lid is not automatically lifted off)
(before start)
lid closed: "0" with flashing point
(rotor not yet identified)
error code: will flash in display
Run time
time selection: - remaining run time to 0
continuous operation (hld):
- run time passed (in hours, minutes)
quick run: - run time passed (as long as button is held;
in minutes and seconds)
Temperature*
run: current sample temperature in °C
(in temperature equilibrium)
* only for instruments with refrigeration unit
Keys
start : normal start of the centrifuge
stop : manual stop of a run
open lid: open lid
quick run: short-term operation of the centrifuge as long as
rpm/RCF
switch: switching between rpm and RCF display
bucket set.: Setting of the bucket number
pretemp : Pre-temp-function*
"set" keys: stepwise increase/decrease of setpoint values
Short pressing of any of the "set" keys: switch from current to preset
value, signaled by flashing display.
Error codes (troubleshooting see chapter "Troubleshooting"):
E-19: Unpermissible rotor
E-20: Rotor not identified
E-29: Motor or rotor blocked
E-31: Overtemperature in motor
E-32: Overtemperature in electronic
E-33: Excessive pressure in the refrigeration unit
E-34: Overvoltage
E-35: Overcurrent
Lift Lid : Smoothly lift the lid
rotor: set speed higher than permissible speed of
the rotor
bAL : imbalance
(possible only with the instrument switched on)
key remains pressed
Contents
Contents
For your safety............................................ 3
Index .......................................................... 93
2
For your safety
For your safety
Heraeus centrifuges are manufactured according to
current technical standards and regulations. Nonetheless, centrifuges may pose danger to individuals and
surrounding if
• they are not used as designed
• they are operated by untrained personnel
• their design is improperly changed
• the safety instructions are not followed
Therefore, personnel involved with operation and
maintenance of the centrifuge must read and follow
the safety instructions.
In addition, the pertinent regulations for prevention of
accidents must be strictly followed.
This manual is an integral part of the centrifuge assembly and must be kept close at
hand at all times.
When damages to the power cord or at
casing are noticed the centrifuge must
to be set out of operation!
Proper use
The centrifuge is designed to separate liquidsuspended materials having different densities and
particle size, respectively (maximum sample density is
1.2 g/cm³ {ml} at maximum speed).
Improper use
During a run, a safety zone of 30 cm around the centrifuge must be maintained where neither persons nor
hazardous materials may be present.
The centrifuge may cause harm to its user or other
persons or may damage goods if safety measures are
not followed
:
Centrifuging hazardous materials
• The centrifuge is neither made inert, nor is it explo-
sion-proof. Therefore never use the centrifuge in an
explosion-prone environment.
• Do not centrifuge explosive or flammable sub-
stances. The same holds for substances prone to
react violently with each other.
3
For your safety
• Do not centrifuge toxic or radioactive substances or
pathogenic microorganisms without suitable safety
systems.
If microbiological samples of risk group II (according
to "Laboratory Bio-safety Manual" of WHO) are being centrifuged, aerosol-tight bio-seals have to be
used.
For materials in a higher risk group, more than one
precaution is required.
• Should toxins or pathogenic substances enter the
centrifuge or its parts, you must perform appropriate
procedures for disinfection (see "Maintenance and
care – Disinfection").
• Strongly corrosive substances that may cause
damage to materials and reduce the mechanical
strength of the rotor may be centrifuged only inside
protective tubes.
Handling the centrifuge
• Use only original accessories for the centrifuge. The
only exceptions are common glass or plastic centrifuge tubes if they are approved for the rotor speed
and RCF values.
• Never use the centrifuge unless the rotor is properly
installed.
• You may use the centrifuge only with a properly
loaded rotor. You must not overload the rotor.
• Strictly follow the rules and regulations for cleaning
and disinfection
• If the rotor or the rotor lid shows signs of corrosion
wear, you must stop using it.
or
• Never open the lid manually if the rotor is still turn-
ing.
• You may use the emergency lid release only in
case of emergency, e.g. during an interruption of
power supply
(see chapter "Troubleshooting").
• Never use the centrifuge with an opened lid.
• Never use the centrifuge if the front panel has been
partially or totally removed.
• Changes in mechanical or electrical components of
the centrifuge may only be carried out by individuals
authorized by Kendro Laboratory Products.
4
For your safety
Conformity to current standards
Heraeus centrifuges are manufactured and tested
according to the following standards and regulations:
- For all voltages
• IEC 61010
- For 120 V only
•
- For 230 V only
•
Please get acquainted with the details of the test standards from the technical data.
Safety instructions in this manual
This symboldenotes potential hazards to
persons.
This symbol denotes potential damage to
the centrifuge or parts in its immediate
surroundings.
General comments are marked with this
symbol.
In addition, you are asked to adhere to the pertinent
regulations, in Germany
• Regulations for prevention of accidents BGV A2
• Regulations for prevention of accidents VBG 5
• Regulations for prevention of accidents VBG 7z
- with cooled devices additionally
• Regulations for prevention of accidents BGV D4
5
For your safety
Notes
6
Description of the Multifuge®
The Multifuge® 1 S / 1 S-R
The figure below shows a Multifuge® 1 S-R with an
opened lid and a swinging bucket rotor installed.
Description
The Multifuge® 1 S-R is a general-purpose tabletop
centrifuge for biotechnological and pharmaceutical
research that moves high capacity centrifugation onto
the fast track. It spins more tubes at higher RCFs more
rapidly than competitive instruments and can process
nominally 1.6 liters of sample in a single run. There are
various rotors available that can achieve high RCFs
and accommodate a wide range of accessories for all
common tube types, micro titer and deep well plates.
The user-friendly "EASYset" control panel permits easy
selection of speed, RCF value, run time and run profile
(acceleration and deceleration), as well as temperature
of the Multifuge
RCF display and vice versa, with a touch of a button
and even during a run.
®
1 S-R. You can switch from speed to
7
Description of the Multifuge®
Safety systems
The Multifuge® is equipped with a number of safety
systems:
• Housing and lid are constructed of 6 mm steel.
• Lid with window
• Lid lock with safety check
You can only open the centrifuge lid when the
power is turned on and the rotor has come to a
stop. You can only start the centrifuge if the lid is
properly locked.
• Automatic rotor identification
• Electronic imbalance detection as a function of rotor
(SMARTspin
TM
)
Do not tamper with the safety
systems!
Parts supplied
Accessories supplied with the centrifuge are:
− power cord
− a special wrench for securing the rotor
(As seen in the picture)
− condensed operating instructions
− corrosion protective oil
The printed documents consist of the delivery notes
and this Manual.
Setting speed by rpm Adjustable from 300 rpm to 15 000 rpm, in 10 rpm increments
RCF selection Upon actuation of RCF switch , the RCF value can then be entered
Time selection
Run time display in "quick run" mode Between 1 s and 60 s in seconds´ steps, above in minutes´ steps
Setting temperature * Adjustable from -9°C to +40°C, in one degree increments
End of centrifugation The speed display will read "end"
(* only with refrigeration unit)
Adjustable in minutes from 1 min to 9 h 59 min,
or extended time mode from 1 min to 99 h,
”hld"-mode: continuous operation
10
Function Feature
Description of the Multifuge®
Lid opening
Start
Stop
"Quick Run" mode
Diagnostic messages
Automatic unlocking via ”open lid" key (
)
(unlocking in case of power failure: see chapter ”Troubleshooting")
Start key (
Stop key (
Pressing the "quick run" key (
)
)
) activates maximum acceleration up to the
maximum permissible speed of rotor; upon key release the centrifuge stops with
maximum deceleration power.
• Alternating display ”Rotor"/maximum speed or RCF
(speed selected exceeds max. speed of the rotor)
• Lid has not been lifted off the lock during opening:
display ”lift lid" (manual lifting of lid is required)
• General instrument malfunction
(error messages with ERROR codes, see "Troubleshooting")
11
Description of the Multifuge®
Notes
12
Before use
Centrifuge transport and installation
After opening the box remove the protective materials.
When transporting the centrifuge,
consider its weight (see "Technical
Data"); always grab it on both sides
ensuring that enough helpers are
around.
Do not lift on the front panel!
Before use
The centrifuge can be damaged by
jolting during the transport!
Transport the centrifuge only in the
upright position using proper containment and secure it properly.
Handle the centrifuge carefully.
Proper location
The centrifuge may only be used indoors. Its location
must meet the following criteria:
• A safety zone of at least 30 cm (12 inches) around
the centrifuge must be maintained. Hazardous materials must not be stored beside the centrifuge during its use.
• The laboratory bench or centrifuge trolley must be
stable and resonance-free. A good support is provided by a laboratory bench or a centrifuge cart with
lockable casters.
• To ensure sufficient air circulation, a minimum dis-
tance from the wall of 10 cm (4 inches) at the back
and of 15 cm (6 inches) on each side must be kept.
13
Before use
• The centrifuge must be protected from heat and
direct sunlight.
• The location must be well ventilated at all times.
UV rays reduce the durability of
plastics.
Protect the centrifuge, rotors and
plastic accessories from direct
sunlight.
Main connection
Connect the centrifuge only to a grounded main power
supply. Make sure that the power cord is compatible
with the valid safety regulations and that your main
voltage and frequency correspond to the specifications
on the instrument label.
Make sure that the centrifuge is switched off (on the
right-hand side of front panel) before connecting
electrical wire to main power supply.
RS232 interface
specifications
Main connection
14
Rotors and accessories
Rotors and accessories
A rotor is not included as part of a Multifuge® centrifuge.
A large variety of rotors are available as accessories.
In addition, there are adapters and reduction sleeves
for a variety of commercially available tubes and
bottles.
For a complete list of available tubes, bottles, adapters and accessories, please refer to our current sales
documentation.
For more information you can visit our web site at
http://www.Kendro.com
Multifuge
During centrifugation, heat is generated by air friction
between the rapidly spinning rotor and the air inside
the rotor chamber. The continuous air flow in the air
cooled centrifuge is restricting the temperature rise of
the samples. The equilibrium temperature rise depends on the ambient temperature, the type of rotor,
the speed, the duration and the number of runs.
®
1 S (air cooled)
Use caution when touching rotors as
they may be hot after long runs at
high speed.
15
Rotors and accessories
Rotors for the Multifuge® 1 S
Table 1: Rotors for Multifuge
Rotor designation Swinging Bucket Rotor TTH 400
®
1 S
Differences of 120V instruments are shown in parentheses
1 S-R Differences of 120V instruments are shown in parentheses
Fixed-Angle Rotor FA12.94 Highconic
6 x 94 ml / 12 x 16 ml
75002006
®
Micro Liter Rotor
48 x 2.0 ml
75003348
21 885 - inner ring
Min temperature at nmax [ °C ]
relative to room temperature 23°C
0 ( 4 ) 4 ( 6 )
Speed at 4°C [ rpm ] 10 350 15 000 ( 14 500 )
Aerosol-tight * Yes Yes
Autoclavable 121°C 138°C
* Tested and approved by CAMR, Porton-Down, UK
23
Rotors and accessories
Adapter
Table 3: Adapter (1)
Adapter and accessories for
rectangular buckets 7500 2001
Centri-Lab® Adapter type A plus
14 x 7 ml blood sampling 13.0 x 117 16.5 56 grey 7500 2022
12 x 15 ml blood sampling 17.0 x 117 19.5 48 white 7500 2021
8 x 15 ml conical 17.0 x 120 22.0 32 brown 7500 2020
Centri-Lab® Adapter type A
40 x 1.5 / 2 ml micro liter tube 11.0 x 45 - 160 black 7500 5335
20 x 7 ml DIN 13.0 x 115 13.0 80 yellow 7500 5321
12 x 15 ml DIN 17.0 x 115 18.3 48 red 7500 5322
4 x 25 ml universal container 22.5 x 100 31.0 16 green 7500 5391
5 x 25 ml DIN 25.0 x 115 25.9 20 orange 7500 5323
2 x 50 ml DIN 34.0 x 120 35.6 8 green 7500 5324
2 x 50 ml conical 29.5 x 120 37.5 8 green-yellow 7500 5386
1 x 50 ml oil test - - 4 nature 7500 5339
1 x 100 ml DIN 44.0 x 120 48.1 4 blue 7500 5325
1 x 150 ml DIN 50.0 x 110 - 4 grey-blue 7500 5326
for cyto-system - - - - 7600 3417
Aerosol-tight caps
Spar seal
1)
d = diameter
* Max. tube length with aerosol-tight cap
Max. tube
dimensions
d1) x length/ *
[mm]
2 pieces, incl. seals and lubricants
4 pieces, incl. lubricants
Cap
diameter
[ mm ]
Tubes
per
rotor
Color
Order no.
7500 2003
7500 2008
24
Rotors and accessories
Table 3: Adapter (2) * Max. tube length with aerosol-tight cap
Cap
diameter
[mm]
Adapter and accessories for
round buckets 7500 2002
Max. tube
dimensions
1)
d
x length / *
[mm]
Centri-Lab® Adapter type E
34 x 1.5 / 2 ml micro liter tube 10.5 x 46 14.0 136 black 7500 7578
18 x 7 ml DIN 13.0 x 123 14.0 72 yellow 7500 7571
10 x 7 ml blood sampling 13.0 x 123 18.4 40 grey 7500 7579
10 x 15 ml DIN 17.2 x 122 18.4 40 red 7500 7572
8 x 15 ml conical 17.0 x 127 23.0 32 brown 7500 7621
5 x 25 ml US-urine 16.5 x 123 27.5 20 orange-red 7500 7581
5 x 25 ml DIN 25.5 x 121 29.0 20 orange 7500 7573
3 x 50 ml universal container 25.0 x 120 32.0 12 green-yellow 7500 7580
3 x 50 ml conical 29.0 x 119 37.5 12 green-yellow 7500 7577
3 x 50 ml DIN 34.5 x 120 36.0 12 green 7500 7574
1 x 100 ml DIN 45.0 x 134 59.0 4 blue 7500 7575
1 x 250 ml bottle 62.0 x 135
400 ml bottle
Aerosol-tight caps
Spar seal
1)
d = diameter
x 135
80.0
2 pieces, incl. seals and lubricants
4 pieces, incl. lubricants
-
-
Tubes
Color
No. of order
per
rotor
4 nature 7500 7622
4
nature 7500 7583
7500 2004
7500 2009
25
Rotors and accessories
Table 3: Adapter (3) * max. tube length with aerosol-tight cap
Cap
diameter
[ mm ]
Adapter and accessories for
BIOshield
®
600 Rotor 7500 2005
Max. tube
dimensions
1)
d
x length / *
[mm]
Centri-Lab® Adapter type A
40 x 1.5 / 2 ml micro liter tube 11.0 x 45 - 160 black 7500 5335
20 x 7 ml DIN 13.0 x 115 13.0 80 yellow 7500 5321
12 x 7 ml blood sampling 13.0 x 117 16.5 48 grey 7500 5330
11 x 15 ml blood sampling 17.0 x 117 19.5 44 white 7500 5327
6 x 15 ml conical 17.0 x 120 22.0 24 brown 7500 5387
12 x 15 ml DIN 17.0 x 115 18.3 48 red 7500 5322
4 x 25 ml universal container 22.5 x 100 31.0 16 green 7500 5391
5 x 25 ml DIN 25.0 x 115 25.9 20 orange 7500 5323
2 x 50 ml DIN 34.0 x 120 35.6 8 green 7500 5324
2 x 50 ml conical 29.5 x 120 37.5 8 green-yellow 7500 5386
1 x 50 ml oil test - - 4 nature 7500 5339
1 x 100 ml DIN 44.0 x 120 48.1 4 blue 7500 5325
1 x 150 ml DIN 50.0 x 110 - 4 black 7500 5326
for cyto-system - - - - 7600 3417
Spar seal
1)
=
1 set, incl. lubricants
Tubes
per
rotor
Color
No. of order
7500 2007
26
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