Kendro CRYOFUGE 6000i, CRYOFUGE 8500i Service manual

Service Manual

Cryofuge 6000iCryofuge

6000i

Cryofuge 8500iCryofuge

8500i

CRYOFUGE 6000i / 8500i

Cat.No.: 75007520 / 75007521 / 75007550 / 7500755175007526 /

PN 12007520

SERVICE

Table of Contents

Section Title Page
1 OPERATING INSTRUCTIONS Cryofuge 6000/8500i

2 SERVICE

2.1 SERVICING SCHEDULE 2-1+2

2.2 TROUBLE SHOOTING 2-3/7

2.3 TEST POINTS ON MAIN BOARD 2-8/10

2.4 ADJUSTMENT OF IMBALANCE SWITCH 2-11

2.5 CLEANING OF INSTRUMENT PARTS 2-12

2.6 ELECTRICAL SAFETY CHECK 2-12

3 FUNCTIONAL DESCRIPTION

3.1 BLOCK FUNCTIONS 3-1

3.2 CONTACTOR PLATE (MAINS INPUT) 3-2

3.3 MAIN BOARD FUNCTIONS 3-3+4

3.4 KEY AND INDICATION BOARD 3-5

3.5 SPEED DETECTION BOARD 3-5

3.6 IMBALANCE SWITCH 3-6

3.7 COOLING PLANT 3-6
COOLING PLANT – FLOW CHART 3-7

4DIAGRAMS

4.1 BLOCK DIAGRAM 4-1/4-3

4.2 WIRING DIAGRAMS 4-4/4-7

4.3 WIRING CONNECTION DIAGRAMS 4-8/4-13

4.4 MAIN BOARD - COMPONENT PLAN 4-14

4.5 MAIN BOARD - WIRING DIAGRAM 4-15/4-22

4.6 KEY AND DISPLAY BOARD - COMPONENT PLAN 4-23

4.7 KEY AND DISPLAY BOARD - WIRING DIAGRAM 4-24

4.8 NOISE FILTER BOARD - COMPONENT PLAN 4-25

4.9 NOISE FILTER BOARD – WIRING DIAGRAM 4-26

4.10 INTERFACE BOARD - COMPONENT PLAN AND WIRING DIAGRAM 4-27

4.11 SPEED DETECTION BOARD - COMPONENT PLAN AND WIRING DIAGRAM 4-28

4.12 FRONT KEYBOARD - COMPONENT PLAN AND WIRING DIAGRAM 4-29

5 REPLACEMENT OF COMPONENTS

5.1 DISMANTLING THE HOUSING 5-1

5.2 DISMANTLING OF SEALING ELEMENTS 5-2

5.3 REPLACEMENT OF MECHANICAL COMPONENTS 5-3+4

5.4 REPLACEMENT OF ELECTRICAL COMPONENTS 5-5+6

5.5 REPLACEMENT OF DRIVE ELEMENTS 5-7

5.6 REPLACEMENT OF CONDENSER FAN 5-8

5.7 REPLACEMENT OF PARTS OF COOLING PLANT - CHARGING INFORMATION 5-8+9

6 BREAK DOWN DRAWINGS AND LIST OF SPARE PARTS

6.1 BREAK DOWN DRAWINGS 6-1/14

6.2 SPARE PARTS LIST

7 CERTIFICATE

8 TECHNICAL BULLETINS

Edition: 01 Contents

26.12.01MH Page: 0-1

7-1/3

Cryofuge 6000i/8500i

SERVICE

Important Note

Technical Information for use of Cryofuges 6000i/8500i

__________________________________________________________________

Cat. No. 75007520 Cryofuge 6000i 3 x 400V 50/60Hz – 4,2kW
Cat. No. 75007521 Cryofuge 6000i 3 x 230V 50Hz – 4,4 kW
Cat. No. 75007526 Cryofuge 6000i Single Phase 230 V 50 Hz – 3,6 kW

Cat. No. 75007550 Cryofuge 8500i 3 x 400V 50/60Hz – 6 kW
Cat. No. 75007551 Cryofuge 8500i 3 x 230V 50Hz – 6 kW

Required door-width : 80 cm
Dimensions : 800 mm x 905 mm without wooden palette
Weight : Centrifuge 430 kg / Rotor 60 kg
Ground pressure : approx. 1450 N/m
Noise Level: CF 6000i - <64 dB(A); CF 8500i – <67 dB(A)

2

Voltage : 3 x 400V, 50/60 Hz, N capable handling
Required Fuses : 32 Amp slow blown
Connection 3 x 400V : Plug Cekon Type CEE 17, 32 A-6 H

3 L + N + PE or directly installed via master power switch

Voltage : 3 x 230V/ 50 Hz
Required Fuses : 32 Amp. slow blown
Connection 3 x 230V : Plug Cekon Type CEE 17, 32 A-9 H

3 L + PE or directly installed via master power switch

Voltage : Single Phase 230 V/50 Hz
Required Fuses: 32 Amp. slow blown
Connection 1 x 230V without plug

Heat rejection to environment : 15.120 kJ/h (14.330,993 BTU) CF 6000i

19.440 kJ/h (18.425,562 BTU) CF 8500i

12.960 kJ/h (12.283,708 BTU) Single Phase

Edition: 01 Contents

26.12.01MH Page: 0-2

Cryofuge 6000i/8500i

®

CRYOFUGES

LARGE VOLUME REFRIGERATED CENTRIFUGES

HS/Cf 1e

CRYOFUGES

LARGE VOLUME REFRIGERATED CENTRIFUGES

Precision for practical work: in blood banks and biotechnology.

Quality Products. Lifetime Care.

®

The Cryofuge 6000i is an attractively
designed, large volume floor standing
refrigerated centrifuge by Kendro
Laboratory Products offering
optimised technology for lowspeed
applications.

It is ideal for use in blood banks, biotech­nology and the pharmaceutical industry,
where large volumes are centrifuged at
high speeds and constant temperatures.
It is also used for routine separation tasks
in laboratories and production facilities.

The use of sophisticated microelectronics
systems and high strength materials for
rotors and accessories guarantees out­standing reproducibility and highly
accurate centrifugation.

With a large 6 litre capacity and a top
speed of 4240 rpm (6010 x g), the
Cryofuge 6000i is designed for a wide
range of separation tasks. It can accom­modate 1.5 ml tubes, 1 litre containers
and quad blood bags.

The Cryofuge 6000i is competitively
priced and comes with a wind-shielded
rotor and an extensive range of accesso­ries. Back up from an international
support and service organisation ensures
maximum performance and reliability.

Typical applications

The Cryofuge is designed, in particular,
for the qualified production of human
blood components in blood banks. It is
also used for blood fractionation, serum
extraction, radio immuno assays (RIA),
cell preparation, fractionating protein and
enzyme precipitates, separating fine crystal
deposits – and in fact, in every situation
where high performance and capacity are
required.

Powerful technology

The Cryofuge is equipped with a power­ful, maintenance free induction drive for

quiet and smooth operation. Its refri­geration system uses the environmentally
friendly CFC free refrigerant R 404a.

Acceleration and braking times have
been optimised for all application require­ments. An automatic digital temperature
compensation system prevents samples
from freezing.

User friendly

A new automatic lid lock enables the
Cryofuge to be closed easily. The ergo­nomically designed keypad allows simple
entry and storage of operating para­meters. Main control functions are
conveniently arranged for easy access,
while the

SEPACONTROL

®

control system
makes operation straightforward. The
"delay" function permits users to delay
system start-up.

Microprocessor control

The Cryofuge is equipped with 33 centri­fugation programs for routine applications.
Nine acceleration and nine braking profiles
ensure reproducibility of all centrifugation
parameters. The Cryofuge is now also
equipped with a profile program for
defining up to 10 cut-off points with time,
speed, braking and acceleration curves.
Speed is controlled digitally with auto­matic RCF adjustment. Centrifuges can
also be connected to a computer for
data recording via a serial interface.

Safety

When designing the Cryofuge, all inter­national safety standards were kept in
mind to guarantee the highest level of
safety. Diagnostic and fault messages are
clearly visible. Pre-selection of buckets
protects against overspeeding. The lid
lock system and steel armoured rotor
chamber further ensure safe operation.
A key operated switch safeguards
against unauthorised use.

Service friendly

The Cryofuge is equipped with a digital
self diagnosis system for monitoring
operability. If a fault is detected, a mes­sage is immediately displayed. Should
an error occur, however, our fast and
efficient international service team can be
relied upon.

The Cryofuge 6000i Refrigerated
Centrifuge is used for the qualified
production of human blood
components in blood banking.

CRYOFUGE 6000i REFRIGERATED CENTRIFUGE

CRYOFUGE 6000i REFRIGERATED CENTRIFUGE

02

Conveniently arranged function
keys and easy to read digital
display for simple operation and
quick access.
Ergonomically designed keypad
allows simple reproduction of
operating parameters.

Keys for controlling basic
functions are also located on
the front of the Cryofuge for
immediate access.

03

CRYOFUGE
6000i GMP
AND 8500i GMP

Wind-shielded rotor and lid, showing a selection of the extensive range of buckets and
adaptors.

CRYOFUGE
6000i GMP
AND 8500i GMP

Convenient operation

The SEPACONTROL operating panel is
systematically arranged, clearly displays
all functional areas and permits easy
access to operating parameters. The
user simply presses a few keys to select
the required centrifugation program and
start the Cryofuge. A design which
eliminates knobs and dials ensures high
reproducibility and easy cleaning of the
control panel.

SEPACONTROL provides users with
up-to-date information on the status of
the system. Visual diagnostic indicators
and acoustic signals supplement the
information supplied by the microcom­puter. This makes it easy to use the
centrifuge right from the start.

Key operated switch

This switch protects programs against
unauthorised access. Depending on the
position of the switch, users are either
permitted to freely program the system,
call up one of the 33 programs in
memory or simply repeat the program
last used.

Panel

Five visual displays facilitate the identifi­cation of problems during centrifugation.
When the lid is opened (check lid), the
rotor is unevenly balanced (imbalance),
in the event of a system error (system
check), programming error (progr. error)
or if the temperature limit has been
exceeded (overtemp), an LED signals a
warning.

Memory control

33 memory locations are available to
save run parameters for future use. New
programs generated using the function
panel can be immediately added to the
library.

Status

The status field is systematically arranged
into the following areas: start-up and
braking curves, speed and rotor data,
time and temperature. LEDs prevent the
user from confusing the values on the
display.

Programming

The programming field permits easy
setting of the required parameters. Only
5 keys are required for completely pro­gramming all parameters! The keys are
used for changing the display setting,
values displayed and confirming input.

Simple, functional operation

The basic functions for opening the
centrifuge lid, starting and stopping the
system and quick-stop are located on
the front of the Cryofuge for immediate
access.

Rotor and accessories

An aerodynamic wind-shielded rotor and
6 different types of bucket ensure maxi­mum performance and flexibility. The wind­shielded rotor is included in the basic
centrifuge package. The vast range of
accessories is listed in the tables overleaf.

Four buckets have been specially devel­oped for centrifuging blood and pro­ducing thrombocyte concentrates. The
system can handle all standard blood
pack systems. Additional accessories
such as plastic inserts and balancing
accessories round off the package.

Two other buckets used in combination
with various adaptors permit all tubes
with nominal volumes between 1.5 ml
and 1000 ml to be centrifuged.

HERANET data documentation

The Heraeus HERANET data documen­tation system is available for data
documentation in accordance with GMP.
Please request our HERANET brochure
from your Kendro representative.

The Cryofuge 6000i GMP (Good Manu­facturing Practice) offers the same perfor­mance and accessories as the Cryofuge
6000i. The Cryofuge 8500i GMP attains a
maximum centrifugal force of 8525 x g
and a maximum speed of 5050 rpm.
GMP Cryofuges are specially designed for
use in clean room applications.

Special features of the Cryofuge
6000i GMP and 8500i GMP

The special encapsulated casing is sealed
to prevent particles from escaping and
contaminating the clean room. The space
between the centrifuge and floor is equip­ped with a rubber gasket. Thanks to its
special smooth finish, the casing is very
easy to clean. The 6000i GMP and 8500i
GMP are equipped with connections for
an external cooling water system; the flow
rate of the cooling water is kept to a mini­mum. On request, the system can be
connected to an external cooling system.
The enclosed and sealed casing and
vibration insulation system ensure low
noise generation during operation. Heat
radiation is also minimised.

Before installing the Cryofuge GMP, please
be sure to contact the Kendro Service
Department.

04

Model Cryofuge 6000i Cryofuge 8500i Technical data
Max speed (rpm) 4,000 4,000 Operation with blood packs double-quint

Max. RCF (x g) 5,312 5,312 No. of pack systems/bucket 2
Max. capacity 2 x 800 ml 2 x 800 ml Polyamide plastic inserts:
Max. perm. mass (g) 3,500 3,500 Order no. M

2)

76007667

Min. temp. at max. RCF* –5 °C –5 °C Opening M (mm) 110 x 57
Max. acceleration time (s) 140 140 Order no. L

1)

76007647

Min. braking time (s) 180 180 Opening L (mm) 110 x 70
Radius (cm) 29.9 29.9 Order no. XL

3)

76007657
Opening XL (mm) 110 x 76
Order no. XXL

4)

76007677
Opening XXL (mm) 110 x 88

* At ambient temp. of 25 °C
M, L and XL plastic inserts come complete with balancing weights 75007645. One set of balancing weights includes
4 weights of 6 and 15 g each. Balancing plates 75005759 can be used to compensate big weight differences. One set
includes 2 plates at 35 and 65 g each.
The balancing insert 75007668 consists of an XL plastic insert, 2 balancing weights and 30 balancing plates, enabling
compensation of up to a full bucket load.

1)

Suitable for triple and quad systems.

2)

Suitable for double systems.

3)

Suitable for quad systems, systems with soft filters.4)Suitable for quad and quintuple systems, systems with filters.

Double bucket for blood packs – Order no. 75007617

Model Cryofuge 6000i Cryofuge 8500i Technical data
Max speed (rpm) 4,240 5,050 Operation with blood packs double-trip.

Max. RCF (x g) 5,768 8,182 No. of pack systems/bucket 2
Max. capacity 2 x 750 ml 2 x 750 ml Opening measurements (mm) 99 x 70
Max. perm. mass (g) 3,700 3,700 Plastic insert order no. 75006695
Min. temp. at max. RCF* –4 °C +6 °C Plastic insert opening (mm) 95 x 60
Max. acceleration time (s) 150 190 Plastic insert material PE
Min. braking time (s) 190 220
Radius (cm) 28.7 28.7

* At ambient temp. of 25 °C
Bucket for blood packs 75006695 comes with plastic insert 75006694.

Double bucket for blood packs – Order no. 75006694

Model Cryofuge 6000i Cryofuge 8500i Technical data
Max. speed (rpm) 4,000 4,000 Operation with blood packs double-trip.

Max. RCF (x g) 5,170 5,170 No. of pack systems/bucket 2
Max. capacity 2 x 750 ml 2 x 750 ml Opening measurements (mm) 99 x 139
Max. perm. mass (g) 3,100 3,100 Plastic insert order no. 75006609
Min. temp. at max. RCF* –5 °C –5 °C Plastic insert opening (mm) 99 x 57
Max. acceleration time (s) 140 140 Plastic insert material PP
Min. braking time (s) 180 180
Radius (cm) 28.9 28.9

* At ambient temp. of 25 °C

Double bucket for blood packs – Order no. 75006801

Double bucket for blood packs
75007617

Double bucket for blood packs
75006801

Double bucket for blood packs
75006694

05

Nominal volume of tube (ml) 1.5 7 7 15 15 15 25 25 50 50 50 50 50 100 150
No. of tubes per adaptor 40 20 12 12 11 6

1)

542262111
No. of tubes per rotor 480 240 144 144 132 72 60 48 24 24 36 24 12 12 12
Type of tube ML DIN B. Coll. DIN B. Coll. Falc. DIN Univ. DIN Falc. Falc. Univ. Oil DIN DIN
Max. length of tube (mm) 45 110 110 111 109 121 100 120 130 120 117 120 S 120 120
Max. tube ø (mm) 11 13 14 17 17 17 25 25.5 35 29.5 29 29.5 S 45 55
Max. cap ø (mm) – 14 18 18 19.5 23 25.9 31 36 37.5 35 37.5 S 48 –
Colour of adaptor black yellow grey red white brown orange green green gr./yel. nat. gr./yel. nat. blue black
Adaptor order no. (7500xxxx) 5335 5321 5330 5322 5327 5387 5323 5391 5324 5386 2261 5389 5339 5325 5326
Stand. tube order no. (7500xxxx) 1163a)9001b)– 9003b)– 2845c)9006

b)

–

9005b)2844e)2844e)– 3113d)9007b)1132

b)

1)

Make sure that the bucket swings out correctly when all places are used.

Type of tube: ML = microlitre tube; B. Coll. = blood collection tube ("Vacutainer", "Monovette", etc.); Falc. = "Falcon

®

" (conical tube); Oil test = oil test tube, pear shaped;
Univ. = universal container (with vertical edge, Falcon type).
Standard tube:

a)

1.5 ml microlitre tubes, b)borosilicate glass, c)12 ml Nunc. screw cap tube, d)oil test tube, pear shaped, e)50 ml Falcon screw cap tube.

Adaptors can be used with tubes with the following shape bottom: F = flat, R = round, C = conical, S = special

Model Cryofuge 6000i Cryofuge 8500i Technical data
Max. speed (rpm) 4,000 4,000 Operation with blood packs double-trip.

Max. RCF (x g) 5,310 5,310 No. of pack systems/bucket 2
Max. capacity 2 x 700 ml 2 x 700 ml Opening measurements (mm) 95 x 55
Max. perm. mass (g) 3,100 3,100 Accessories:
Min. temp. at max. RCF* –5 °C –5 °C Metal handles 75006728
Max. acceleration time (s) 140 140 Rubber spacer for shorter
Min. braking time (s) 180 180 packs fitted with
Radius (cm) 29.7 29.7 handle 75006728 76014725

* At ambient temp. of 25 °C

Double bucket for blood packs – Order no. 75006680

Additional accessories for blood pack operation
Order no. 75006683 Pack of "shoe horns" for inserting blood packs,

incl. 1 set of rubber rings

Order no. 75006684 Set of non stick plastic bags (150 each) for smooth insertion and removal of

blood packs

Order no. 75006681 12 rubber volume compensation plates
Order no. 75005759 4 rubber balancing plates

Model Cryofuge 6000i Cryofuge 8500i
Max. speed (rpm) 4240/3006 5050/3006

Max. RCF (x g) 4984/2506 7070/2506
Max. capacity 24 x 15 ml/3 racks

1)

24 x 15 ml/3 racks

1)

Max. perm. mass (g) 2500/1800 2500/1800
Min. temp. at max. RCF* –4 °C +6 °C
Max. acceleration time (s) 150 190
Min. braking time (s) 190 220
Radius (cm) 24.8 24.8

* At ambient temp. of 25 °C

1)

Boehringer Mannheim or Hitachi-5 sample racks

Double rectangular bucket – Order no. 76008078/75015501

Double bucket for blood packs
75006680

Double rectangular bucket
76008078 with adaptors for stan­dard tubes

Double rectangular bucket
76008078

Accessories for standard tubes in double bucket 76008078

06

C F/R F/R F/R CF/R F/R F/R S R RF/CC F/R F

Round bucket 75008165 with
adaptors for standard tubes

Adaptor for large volume tubes
for round bucket 75008165

Model Cryofuge 6000i Cryofuge 8500i
Max. speed (rpm) 4,240 5,050

Max. RCF (x g) 6,010 8,525
Max. capacity 1 x 1000 ml 1 x 1000 ml
Max. perm. mass (g) 3,200 3,200
Min. temp. at max. RCF* –4 °C +8 °C
Max. acceleration time (s) 150 190
Min. braking time (s) 190 220
Radius (cm) 29.9 29.9

* At ambient temp. of 25 °C
Aerosol tight cap (order no. 75008081) available on request.

Round bucket – Order no. 75008165

Nominal volume of tube (ml) 250 2502)250 500 650 750 1000 1000 Bp. DACS
No. of tubes per adaptor 11111–1–1 1
No. of tubes per rotor 666666666 6
Type of tube DIN Div./H. Corni. H DIN H H HST Sing.-trip. DACS
Max. length of tube (mm) 190 190 190 190 1953)148 190 175 – –
Max. tube ø (mm) 59 62.5 61.5 70 84 100 100 99.5 – –
Max. cap ø (mm) –––––8787–– –
Colour of adaptor nat. nat. nat. nat. nat. - - - nat. black
Adaptor order no. (7500xxxx) 66494)8144 8147 8145 66376)– – – 6639 5254
Stand. tube order no.(7500xxxx) 1135b)7894 – 7718 11587)8149 6613 6640 – –

Nominal volume of tube: Bp. = blood pack, DACS = Density Associated Cell Sorting (DACS™ 300, Fresenius)
Type of tube: Div. = diverse, Corni. = Corning bottle, H = Heraeus tube, HST = Heraeus stainless steel tube
Standard tube:

b)

borosilicate glass.

2)

Additional pad for conical tubes required from manufacturer, 3)Only use without cap 75008081,

4)

Pad 75001808 required, 6)Pad 75001913 required, 7)max. speed – 2,600 rpm

Adaptors can be used with tubes with the following shape bottom: F = flat, R = round, C = conical, S = special

Accessories for large volume tubes in
round bucket 75008165

Round bucket 75008165

Nominal volume of tube (ml) 1.5/2 7 7 15 15 15 25 50 50 100
No. of tubes per adaptor 48 35 19 19 17 12 7 4 4 2
No. of tubes per rotor 288 210 114 114 102 72 42 24 24 12
Type of tube ML DIN B. Coll. DIN B. Coll. Falc. DIN DIN Falc. DIN
Max. length of tube (mm) 42 177 177 177 177 177 177 177 177 177
Max. tube ø (mm) 11 13 14 17 17 17 25 35 29.5 45
Max. cap ø (mm) – 14 18.5 18.5 20 23.7 31 39 39 47.5
Colour of adaptor black yellow grey red white brown orange green gr./br. blue
Adaptor order no. (7500xxxx) 8132 8133 8134 8135 8136 8137 8138 8140 8141 8142
Stand. tube order no. (7500xxxx) 1163

a)

9001

b)

– 9003

b)

– 2845

c)

9006

b)

9005

b)

2844

e)

9007

b)

Type of tube: ML = microlitre tube; B. Coll. = blood collection tube ("Vacutainer", "Monovette", etc.); Falc. = "Falcon" (conical tube).
Standard tube:

a)

microlitre tubes, b)borosilicate glass, c)15 ml Falcon screw cap tube, e)50 ml Falcon screw cap tube.

Adaptors can be used with tubes with the following shape bottom: F = flat, R = round, C = conical.

Accessories for standard tubes in round bucket 75008165

07

F/C F/R F/R F/R F/C RF/R F/R F/R F/C

SFFF

C

FR

F

3)

R

S

S

TECHNICAL DATA

Model Cryofuge 6000i Cryofuge 8500i
Description Large volume refrigerated Large volume refrigerated

floor standing centrifuge floor standing centrifuge

Max. speed 4240 rpm 5050 rpm
Max. RCF 6010 x g 8525 x g
Max. capacity 6 x 1000 ml bottles or 12 blood pack 6 x 1000 ml bottles or 12 blood

systems of 800 ml each pack systems of 800 ml each

Control and drive High performance induction drive, High performance induction drive,

SEPACONTROL with SEPACONTROL with
microprocessor microprocessor

Acceleration and
braking profiles 9/10 profiles 9/10 profiles
Runtime 1 min – 99 hrs, continuous operation 1 min – 99 hrs, continuous operation
Program memory 33 user centrifugation programs, one of which with freely combinable

braking and acceleration curves, key operated switch for protection
against unauthorised access, data last used saved, in the case of a
power interruption data saved for unlimited period of time.

Temperature control range –20 °C to +40 °C –20 °C to +40 °C
Safety features Lid lock and interlock, imbalance cut-out, steel armoured chamber
Design Sturdy, torsion resistant steel design with stainless steel rotor chamber
Dimensions (hxwxd) 1178 x 800 x 905 mm 1178 x 800 x 905 mm
Weight (excl. rotor) 445 kg 445 kg
Power consumption 4.2 kW 5.4 kW
Refrigeration unit 1.9 kW 1.9 kW
Recommended fuse 25A 25A
Designed and tested IEC 1010, UVV VBG 20, UVV VBG 7z, UVV VBG 4, DIN 58970 part 1,2,
in acc. with 4, radio interference suppression in compliance with VDE 0871 B

Rotor
Wind-shielded rotor 75006606 75006606

included in basic package included in basic package

Order number Cryofuge 6000i Cryofuge 8500i

220 V/3 Ph/50 Hz 75007521
230 V/1 Ph/50 Hz 75007526
400 V/3 Ph/50 Hz 75007520

GMP model

400 V/3 Ph/50 Hzwith built-in refrigeration unit 75007562 75007561

with external refrigeration unit 75007566

ORDER NUMBERS

The Cryofuges 6000i and 8500i
are now even more convenient
to operate with a load-relief lid
for easy opening and closing.

TECHNICAL DATA

ORDER NUMBERS

Australia Kendro Laboratory Products · Lane Cove DC · NSW 2066 · Tel. [phone] · Fax. [phone] · [e-mail]
Austria Kendro Laboratory Products GmbH · Wien · Tel. [phone] · Fax [phone] · [e-mail]
Canada Kendro Laboratory Products International Sales · Newtown · USA · Tel. [phone] · Fax. [phone] · [e-mail]
Denmark Kendro Laboratory Products AB · Albertslund · Tel. [phone] · Fax [phone] · [e-mail]
France Kendro Laboratory Products · Courtaboeuf cedex · Tel. [phone] · Fax [phone] · [e-mail]
Germany Kendro Laboratory Products GmbH · Hanau · Tel [phone] · Fax: [phone] · [e-mail]
India Kendro Laboratory Products (India) Pvt. Ltd. · New Delhi · Tel. [phone] · Fax [phone] · [e-mail]
Italy AHSI S.p.A. · Cavenago Brianza · Tel. [phone] · Fax [phone] · [e-mail]
Poland Kendro Spólka z.o.o.

· Warszawa · Tel. [phone] · Fax [phone] · [e-mail]

Portugal Heraeus S.A. · Cacem · Tel. [phone] · Fax [phone] · [e-mail]
PR China Kendro Laboratory Products (H.K.) Ltd. · Hong Kong SAR · Tel. [phone] · Fax [phone] · [e-mail]
Spain Heraeus S.A. · Madrid · Tel. [phone] · Fax [phone] · [e-mail]
Sweden Kendro Laboratory Products AB · Upplands Väsby · Tel. [phone] · Fax [phone] · [e-mail]
Switzerland Kendro Laboratory Products AG · Zürich · Tel. [phone] · Fax [phone] · [e-mail]

Kendro Laboratory Products SA · Carouge-Genève · Tel. [phone] · Fax [phone] · [e-mail]

U.K./Ireland Kendro Laboratory Products Limited · Bishop’s Stortford · Herts · Tel.[phone] · Fax [phone] · [e-mail]
USA Kendro Laboratory Products L.P. · Newtown · Tel. [phone] · Fax. [phone] · [e-mail]

All other International Sales in Europe, Middle East, Africa

Kendro Laboratory Products International Sales · Hanau · Germany · Tel. [phone] · Fax [phone] · [e-mail]

Asia Pacific Kendro Laboratory Products (H.K.) Ltd. · Hong Kong SAR · Tel. [phone] · Fax [phone] · [e-mail]
Latin America Kendro Laboratory Products International Sales · Newtown · USA · Tel. [phone] · Fax. [phone] · [e-mail]

Internet http://www.kendro.com .http://www.heraeus-instruments.com

Kendro Laboratory Products – a worldwide company formed by the merger of Heraeus Instruments and Sorvall.

Should your country not be included in the address list, please contact Kendro Laboratory Products International Sales in Germany or USA.
Subject to change without notice. Printed in Germany 6C 07/99 VN 4t Frotscher

SERVICE

2. SERVICE

2.1 Servicing Schedule (Yearly Procedure)

2.1.1 Maintenance Routine without Dismantling the Centrifuge

1. Electrical Installation and Safety

Switch OFF the centrifuge and disconnect the unit from power.
Check voltage supply and mains fusing (3 x 32 Amps slow blow characteristic or other for
corresponding line voltages)
Check condition of plug and wall socket - replace defective parts.
Check main contactor for burnt out poles.
Check cord condition and fixing - replace or refit it.

2. Location and Mechanical Installation

Check the base for resonance-free and stable conditions.
Check for a well ventilated place and sufficient distances to walls or adjacent equipment (min.
30 cm), avoid direct sunlight.
Check the levelling of the centrifuge - use a spirit level.

3. Lid Locking Mechanism and Safety Device

Connect the centrifuge to power and switch ON
Check for easy lid closing and self-acting lid opening - if in disorder, re-adjust lid looped hooks
and/or hinges and/or move lid lock assembly aside and smear hooks slightly with grease.
Check the central rubber gasket for lid sealing and replace it, if damaged.
Open the lid and turn the rotor by hand, then close the lid and try to open it using the lid key: the
lid must not be opened as long as the speed values (> 10 rpm) are indicated - if safety circuit is
out of function, replace main board.

4. Cleanliness of Spin Chamber and Motor Cover

Open the lid and remove the rotor: for loosening turn rotor's collar nut with use of 19 mm fixed
spanner anti clock wise (in arrow direction - see rim of rotor bowl), thereby hold the rotor on it's
cross.
There is an additionally turn resistance after approx. one revolution which has to be overcome
with an further lever pressure of the fixed spanner. Then turn the drive shaft clockwise with the
10 mm socket wrench as long as the rotor can be lifted up. Two persons are necessary to seize
the rotor by it arms on opposite and to lift the rotor carefully out of the bowl.
Clean the wind-shield with a dry and absorbent cloth (remove all dust and moisture - see also

2.5 Cleaning)
Check the correct seat and condition of the motor cover.
Take care of the cleanliness of the annular slot around the motor shaft: there is shaft packing of
felt which has to be soaked with oil. - Penetrating fluids can damage the upper motor bearing.

5. Rotor and Bucket Condition and Sealing

Check the condition of rotor and buckets (especially all supporting or stressed partitions as jib
arms, rim of the bucket's bearing surface etc.): the rotor and/or buckets must not be used any
longer, if there are visible traces of mechanical damage or rust.
Check the condition of rotor and/or bucket sealing and replace them in case of malfunction

6. Rotor Fixing and Motor Shaft

Check the condition of the drive motor shaft: the centrifuge must not be used any longer, if the
drive shaft is damaged (bend, thread is worn out, horizontal grooves etc.)

7. Temperature Control Circuit

Clean the condenser (use a vacuum cleaner).
Check the thermal isolated fixing of the temperature sensor.
Re-install the rotor and run the centrifuge at 2000 rpm (1500 rpm with buckets #7719) and at
4°C
Check the temperature 30 minutes later: the displayed and measured value should be
approximately the same (tolerances +/-2K)

Edition: 02 Service

18.04.02 MH Page: 2-1

Cryofuge 6000i/8500i

SERVICE

2.3.2 Maintenance Routine after Dismantling the Centrifuge Casing (see Section 5)

1. Motor Supporting Elements

Check the supporting and damping motor elements (enlarged rubber abrasion, often imbalance
runs) and replace them by worse condition or at least every 3 years.

2. Imbalance Switch

Check the function of the imbalance switch and replace in case of malfunction.

3. Braking Circuit

Check the function of the braking circuit (warming up of brake resistor, even and noiseless
brake effect) and replace defective parts in case of malfunction.

4. Lead and Screwing Connections

Check the terminal and plug connections of leads on all boards and electrical components,
replace defective parts.
Check the screwing connections of boards, mechanical and electrical components and tighten
them if necessary.

5. Protection Earth Core and Grounding Connections

Check the protection earth core for continuity and all grounding plug connectors (see also 2.6
Electrical Safety Check).
Check insulation resistance and measure the substitutional leakage current (see 2.6)

Edition: 02 Service

18.04.02 MH Page: 2-2

Cryofuge 6000i/8500i

SERVICE

Trouble Shooting

Error Indication Error Cause Possible

Error Source

"check lid" LED on,
interval sound

circuit for lid switches

"check lid" LED on and
"OPEn"

"imbalance"

LED on, interval sound

2) base is not sturdy

3) centrifuge is not

4) disjusted or faulty

5) leads to imbalance

6) faulty component of

"overtemp"
LED on, interval sound

2) without programming

E-00 appears
system check

LED on and interval sound

2) motor is jammed 2) remove drive, replace

faulty speed signal

2) faulty processing

motor didn't start 1) connection: drive to

2) defective drive 2) check resistance of

3) faulty main board 3) replace main board

lid opened
mechanically
during run
FORBIDDEN!

interrupted

during run

15 V circuit for lid switches
is interrupted

at standstill

imbalance circuit has
released

just after starting: drive is

switched off

during longer operation:

rotor coasts to standstill
without braking

rotor didn't turn 1) rotor is jammed 1) check for easy

easy check:

turn rotor by hand and
close lid -
speed values must be
indicated

mechanical
emergency lid
opening must only be used
at standstill

lid switches or leads are
interrupted

1) leads to lid switches
interrupted or defective
lid switch

1) rotor not symmetrically
loaded

enough and comes into
vibrations

correctly levelled

imbalance switch

switch are interrupted

imbalance circuit

∆t alarm Rotor chamber must be

1) ∆t is programmed 1) check minimum

of ∆t

1) faulty plug or lead
connections or faulty
speed detection board

circuit on main board

main board

Corrective Procedure

close lid immediately,
turn power OFF/ON,
press start to continue run,
press stop to finish run

check leads to the lid
switches, if micro switch is
faulty, change lid lock
assembly

1) check leads to lid
switches, if micro switch
is faulty - replace lid lock
assembly

1) open lid, check load,
close lid again and start

2) change or reinforce the
base

3) level the centrifuge drive
correctly, use a spirit level
on top of installed rotor

4) readjust imbalance
switch or replace in
case of malfunction

5) check leads with
ohmmeter and replace if
faulty

6) replace main board

precooled before ∆t
activation

reachable temperatures
at maximum speed and
20°C, check cooling
plant in case of big
deviation

2) check compressor and
cooling plant (loss of
refrigerant)

movement, remove any
jamming objects

faulty parts

1) check plug contacts
and leads - measure
speed detection signal
on XW1, XW3 - replace
faulty parts

2) replace main board

1) check terminal and
lead connections,
replace faulty parts

motor windings, replace
faulty parts

Edition: 02 Service

18.04.02 MH Page: 2-3

Cryofuge 6000i/8500i

SERVICE

Trouble Shooting

E-02 flashes
system check

LED on and
interval sound

E-03 flashes
system check

LED on and interval sound

E-04 flashes
system check

LED on and interval
sound

2) defective amplifier or

E-06 flashes
system check

LED on and interval sound

2) faulty tracks or

E-08 flashes
System check

LED on and interval sound

2) faulty intermediate

2) faulty frequency

E-10 appears
system check

LED on and interval sound

E-12 flashes
system check

LED on and interval sound

E-15 appears
system check

LED on and interval
sound

E-17 appears
system check

LED on and interval sound

2) defective lid lock

E-22 appears
system check

LED on and interval sound

program sequence
disturbed

faulty speed signal

during run

faulty temperature
measuring

data lines to keyboard
disturbed during operation

over voltage in DC

intermediate circuit

over current in DC

intermediate circuit

over temperature of the

electronics

program sequence
disturbed

temperature signal
disturbed

program sequence
disturbed

micro switch didn't open
after pressing lid key

NV-RAM and micro
controller can not
cooperate

bad ground connection check all ground

connections,
tighten loose screws

see E-00
or e.g. missing rotor

1) faulty temperature
sensor (PT100) or leads

converter test:

substitute PT100 by

100Ω and 120Ω

1) faulty plug contact or
connecting leads

components

1) leads to brake resistor
interrupted or defective
resistor

circuit

1) defective motor 1) disconnect the motor,

converter

insufficient cooling of
power electronics

NV-RAM not initialised insert the correct NV-RAM

see E-04 see E-04

reading error of NV- RAM
(checksum)

1) faulty leads or micro
switch

micro controller or NV­RAM are mixed up

wait until rotor has come to
standstill, then see E-00

1) check plug connection
and resistance of

PT 100, replace faulty

sensor

2) set the temperature to
20 °C and watch the
display: after power ON
100Ω ->(0±2)°C
120Ω ->(50±2)°C
replace defective main
board

1) replace plug contacts
or connecting leads

2) change key and
indication board or main
board

1) check leads and brake
resistor, replace defective
parts

2) replace main board

check motor windings,
replace defective motor

2) replace main board

check wall distance, air
ventilation and fan
function, change bad
conditions, replace
defective parts

if error indication occurs
repeatedly replace NV­RAM

1) replace defective parts

2) replace lid lock
assembly
check the correct
identification
No's of micro controller
and NV-RAM

Edition: 02 Service

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Cryofuge 6000i/8500i

SERVICE

Trouble Shooting

E-23 appears system
check

LED on and interval sound

2) defective driving of

2) F4: motor over

3) F5: over pressure

2) faulty driving of

displays remain dark no mains voltage supply

2) defective mains cord

2) faulty keyboard 2) replace keyboard

3) faulty main board 3) replace main board
interrupted program

all displays elements are
shortly illuminated

2) bad or missing

mains contactor didn't

pull up, hour counter
doesn't run

mains contactor has been
cut off, rotor coasts to

standstill without braking

mains contactor has
pulled, hour counter runs,

rotor doesn't start or
coasts to standstill without
braking

no low voltage supply for

key and display board

sequence

processor reset

caused by
electromagnetic
disturbance

1) interruption of the
mains security path

mains contactor

main security path is
interrupted by one of the
safety elements:

1) F3: compressor’s over
current protection

temperature switch

switch of cooling plant

1) missing mains phase
for supply of frequency
converter or defective 35
slow blown fuse

preloading for
intermediate circuit

1) Phase L2 or fuse F2
failed

or mains switch

1) faulty connection from
main board to key board

NV-RAM out of socket or
not correctly inserted

1) reduced voltage
supply (< 10%)

ground connection

1) check F3,F4,F5 and
closing contacts of K2
inclusive all connecting
lines and replace
defective parts

2) replace contactor or
main board

1) F3: check main
phases, measure the
compressor current and
compare with adjustion
of protection switch,
check compressor
windings, replace
defective compressor

2) F4: check air
ventilation and motor
windings, replace
defective parts

3) enlarge the lateral wall
distance, clean conden­ser, replace defective
fan or make clogged
injection valve free

1) check mains phase or
replace defective fuse, if
it blows again check for
other faults (drive
resistance's,
intermediate circuit),
replace defective parts

2) check the switching
action of relay K1 and
replace main board in
case of malfunction

1) check phase and fuse
or circuit breaker and if
faulty, replace or switch
on again

2) check leads and
switch, replace defective
parts

1) check plug
connections on main
and key board, check
connecting leads ­replace faulty parts

insert the right NV-RAM
correctly

1) if the voltage supply
drops often, use a
voltage stabiliser

2) check all ground
connections and
screwings of main board

Edition: 02 Service

18.04.02 MH Page: 2-5

Cryofuge 6000i/8500i

SERVICE

Trouble Shooting

drive makes noises -

no good centrifugation
results

2) motor bearing 2) replace motor

2) defective driving

drive accelerates but
doesn't brake normally -

(noisy braking)

2) defective brake control

mechanical

electrical

faulty brake circuit 1) defective lead or brake

1) wear out of motor
rubber mounts

1) defective terminal
connection or faulty lead
or mot. winding

circuit

resistor

circuit

1) replace motor rubber
mounts (damping
elements)

1) check voltage on
motor terminal (see test
points on main board),
replace defective parts

2) replace main board

1) check connection and
leads and brake resistor
25 Ω , replace faulty
parts

2) change main board

Edition: 02 Service

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Cryofuge 6000i/8500i

SERVICE

2.3 Test Points on Main Board

Test Points Unit Value Condition

1) mains terminal XT

(L2 for low voltage supply)

2) mains terminal L1 to N (or 2

phase)(intermediate circuit)

terminal XM 1 to XM 3
motor voltage measured

inter 2 motor leads ­voltage values must be the same
between all 3 motor leads after the
selected speed is reached

motor current

cable 1
cable 2
cable 3

max. acceleration current

intermediate circuit voltage UD

bridge rectifier V49

current of intermediate circuit

through control resistor R29

measured as voltage drop:
U = I x R

motor winding resistance

inter 1 -> 2, 2 -> 3 and 1 -> 3

plug XD

resistance of brake resistor

plug XE

imbalance switch
voltage drop inter pin 1 and 4

plug XW

voltage drop
speed detection board
inter pin 1 and 3:
inter pin 1 and 2:

terminal XC

resistance of lid solenoid

terminal XC1/XC2

nd

230 V AC

230 V AC (-/+10%)

260 V AC
255 V AC
245 V AC

8.0 A

8.0 A

8.0 A

15 A rotor 75006606 with buckets 75008078

320 V DC
355 V DC

300 V DC
280 V DC

8.5 mV DC
31 mV DC

103 mV DC
250 mV DC

-12 mV DC

0.5 Ω

25 Ω

6.5 V DC
12 V DC

0 V DC

12.0 V DC

10.7 V DC

0.3 V DC

120 Ω

8 Ω

all given values refer to 230V

rotor 75006606 with buckets 75008078
1 000 rpm
2 500 rpm
4 240 rpm, acc. time: 95s, dec. time:
180s

soft iron or moving-iron or digital
effective current measuring instrument
rotor 75006606 with buckets 75008078
set speed = 4 240 rpm

(in the speed range of 2000 to 3000
rpm)

at standstill
during braking phase

rotor 75006606 with buckets 75008078
set speed = 4 240 rpm
shortly by max. acceleration

rotor 75006606 with buckets 75008078
set speed = 1 000 rpm
set speed = 2 500 rpm
set speed = 4 240 rpm
shortly by max. acceleration
shortly during braking phase

motor temperature: 20 °C

resistor temperature: 20 °C

installation:
mechanically closed,
electrically opened

plug inserted, open switch (no
imbalance)
plug removed, or leads interrupted
(imbalance message)
switch closed (plug re-inserted, active
imbalance signal)

active circuit
UB

transistor is cut in (slot position)
transistor is cut out

speed signal: two pulses per revolution

2 x 240Ω parallel – old version
latch motor pin 3 to 4

Edition: 02 Service

18.04.02 MH Page: 2-7

Cryofuge 6000i/8500i

Test Points on Main Board

plug XA

micro switches for lid locking

terminal XB

motor over temp. switch

terminal XN

compressor thermal over current
switch

terminal XO

pressostat

plug XU

voltage drop on PT100

compressor terminal box

1) voltage

2) current

3) resistance of compressor
winding

between C + R (C + P)
between C + S (C + A)
between R + S (A + P)

SERVICE

30 V DC

230 V AC

230 V AC

230 V AC

95 mV DC

3 x 400 V AC; 3 x 230 V AC;

single phase 230V

(depending on version)

3 x 3.8 A (400 V)
3 x 7,5 A (230 V)

7,5 A (single-phase)

3 x 400 V ; 3 x 230 V; 1 x 230V

6 Ω 1,8 Ω 1,9 Ω
6 Ω 1,8 Ω 6,4 Ω
6 Ω 1,8 Ω 7,9 Ω

by open lid

contact open

contact open

contact open

20 °C temperature indication

if contactor K3 has pulled

after 2 minutes run at room
temperature at 20 °C

compressor temperature 20 °C

Edition: 02 Service

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Cryofuge 6000i/8500i

SERVICE

2.4 Adjustment of Imbalance Switch

2.4.1 Meaning of Imbalance Switch

The imbalance switch has the task to switch off the centrifuge by incorrect loading of the rotor before
reaching the normal speed.

NOTE!

1) Before starting with the re-adjustment procedure check the greasing of rotor trunnions and
cleanliness of bucket groves. Opposing buckets and racks must be identical and equal in
weight.

2) Check the motor's rubber mounts and the levelling of the drive with installed rotor (use a
spirit level).

2.4.2 Adjusting Procedure

Tools and accessories:

Rotor 75006606 with buckets 75008078
check weights: 40g + 80g

Procedure

Disconnect unit from power and open the front door.
Loosen both M6 screws on bottom of mounting angle of imbalance switch to keep it movable.
Pull imbalance assembly away from the drive.
Move the assembly very slowly in direction to the drive until a clicking noise is audible.
Refix both screws and start the test run.

2.4.3 Test Run Performance

Install the rotor 75006606 with all buckets 75008078.
Load one bucket with the check weight of 80g.
Close the lid, select acceleration curve 9 and then press the start key.
The imbalance message "bal" must appear when passing 1400 rpm.
If no imbalance signal occurs, correct the adjustment by pulling the assembly a little away from

the drive.
Exchange now the 40g weight and repeat the procedure.
The rotor must reach the max. programmed speed without imbalance signal

NOTE!
Both imbalance tests (with 40 and 80 grams) must be performed with in the buckets 1, 3 and 5 that
means 6 test runs are necessary.

Edition: 02 Service

18.04.02 MH Page: 2-9

Cryofuge 6000i/8500i

SERVICE

2.5 Cleaning of Instrument Parts

ATTENTION - WARNING!
Do not clean the electrical and electronic components with moist cleaners!
For Cleaning the housing or accessories see Operating Instructions section 1 §10.

1) Electronic components
Clean dusty components carefully with a dry and soft brush.

2) Fans
Scratch off carefully with a knife or similar tool the crusted dirt from the fan blades. Resulting
grooves or marks must be removed subsequently with a fine abrasive cloth. Loose dirt is to remove
with a vacuum cleaner.

3) Condenser of the cooling unit
The vents between the heat air deflectors have to be vacuum cleaned. By persistent dirt, the unit must
be carried to an appropriate place where the condenser can be cleaned from the inside to the outside
with compressed air.

2.6 Electrical Safety Check

ATTENTION!
A final electrical safety check must be performed after each maintenance and/or repair.

Resistance check of protective conductor

The resistance between the mains plug's earthing pin and the earthing conductors of the
motor, electronic chassis and the casing must not exceed 200 mΩ.

Isolation resistance Check

Check also the isolation resistance between the poles of the mains plug and the earthing
conductor; the resistance value must be higher than 2 MΩ.

Accessible current measured to EN 61010

The accessible current must not exceed 3.5 mAmps in single fault condition (interrupted
protection earth wire)! In accordance with the EN61010, IEC1010 and UL3101 such a fault
condition can be reproduced by the following measuring circuit.

Steckergehäuse / plug-in casing

10k

Ω

5%

0,022µF

5%

1500

5%

0,22µF

Ω

5%

mV

AC

500

Ω

1%

L3 N

L2

L1

PE

Spezifaktionen für Meßgerät

- TRMS, DC - 5kHz oder mehr

- Eingangswi derstand > 1M

- Toleranz 5% oder besser

- Crest Faktor 5 oder besser

Edition: 02 Service

18.04.02 MH Page: 2-10

Körperstrom:

accessible current:

U = 1750mV I = 3,5mA

max

Ω

I [mA] = U [mV] / 500:

≡

max

Specifaction for the meter

- TRMS, DC - 5kHz or more

- Input resistance > 1M

- Tolerance 5% or better

- Crest Factor 5 or better

Ω

Cryofuge 6000i/8500i

Service

3. FUNCTIONAL DESCRIPTION

3.1 Block Functions

The Cryofuge 6000i/8500i is a microprocessor controlled and programmable floor standing
centrifuge with cooling plant and induction drive motor.

Following boards and components are located inside the unit (see also block diagram section 4
page 4-1):

Mains switch S1.

Mains input angle plate with fuses F1, F2, contactors K2, K3 and hour counter h.

Main board with central processor and ASIC80 component.

Indication board with key and display processor (SEPACONTROL programming) with key
switch for protection of all program parameters.

Serial interface board (RS 232) with plug connection for transferring the operational
parameters to a PC or Heranet system.

Driving stage for mains contactor K2

Main security path with protection for motor overtemperature F4, thermal compressor overload
F3 and overpressure of cooling plant F5.

Noise filter board.

Three phase induction drive motor with an integrated thermal overtemperature switch (cut-out
at 140°C).

Speed detection board is mounted underneath the drive motor.

Imbalance switch S6 mounted aside and in front of the drive motor (for adjustment see section
2 page 2-9).

Two lid lock assemblies each with motor locking system and integrated micro switches (S2, S3
and S4, S5).

Temperature sensor V1 (PT100).

Driving stage for compressor contactor K3 (cuts in compressor M2).

Heating element as brake resistor R1 with integrated fan M6 (mounted behind the casing's
back panel).

Three more Fans for cooling

a) of condenser, drive and compressor (M3)
b) of condenser also (M4, not used for models with water cooling)
c) of power electronics on main board (M5)

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Service

3.2 Contactor Plate (Mains Input)

The following components are mounted on an angle plate, they are accessible after opening the
front door (see also wiring connection diagram in section 4 page 4-9 from right to left):

Terminal for protection earth (PE) connection of mains cable (yellow green).

Terminal for neutral line (N) of mains cable (blue, 400 V version only).

Screw fuse F2 (6 Amp slow blown) for protection of control circuit connected to mains phase
L2 (point 3 on mains contactor) via mains switch S1.

(Second 6 Amp fuse slow blown at 3 x 230 V units)

Screw fuse F1 (20 Amp slow blown) for protection of DC intermediate circuit for the drive's
power electronics connected to mains phase L1 via noise filter board.

(Second 20 Amp fuse slow blown at 3 x 230 V units)

Mains contactor K2 with 3 phased mains connection (points 1,3,5) to supply

1.) DC intermediate circuit for drive's power electronics on phase L1 (point 2)

2.) 3 pole compressor contactor K3 (terminal 2,4,6 and 1,3,5 supply via mains
contactor) to switch the compressor ON and OFF via the thermal over current fuse F3
(manual reset).

Thermal over current fuse F3 attached on compressor contactor's output to protect the
compressor indirectly for thermal overload (part of the mains security path, points 95,96).
Selected to running current of the compressor

Two double RC combinations are fixed with tape (bilateral adhesive) on both sides of the
compressor contactor to reduce the voltage spikes when switching the compressor ON and
OFF (bypassing K3 and F3 contacts).

Hour counter h is parallel connected to the main contactor's coil to determine the operational
time when the centrifuge is switch ON and the lid is closed. This is generally the possible
condition for the compressor's operation (standstill cooling).

Edition: 01 Functional Description

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Service

3.3 Main Board Functions

The main board is mounted beneath the operational panel behind the unit's back wall. The
components on main board are arranged in following groups (see wiring diagram).

Triac stage for coil of compressor contactor K3

Triac stage for fan M4 of cooling plant (condenser)

Triac stage for both parallel connected lid solenoids Y1 and Y2 (old mechanical latch)

Triac stage for three parallel connected fans (M3 for condenser and drive, M5 for electronics
on main board and M6 for brake resistor)

Triac stage for coil of main contactor K2 and hour counter h via main security path (F3, F4
motor overtemperature switch and F5 high pressure control switch)

Driver of new style motor latch system

Noise filter block (in interaction with the external noise filter board) for suppression of radio
interference, caused by the fast switching power electronics (<10 kHz), in accordance with the
German Regulation VDE 871, grade B

Bridge rectifier for DC intermediate circuit supplying brake control path and frequency
converter

Power pack for 3 different low voltage levels

Microcontroller (central processing unit) in interaction with ASIC80 functions for frequency
converter, speed and imbalance detection, temperature measuring and calibration

3.3.1 Power Pack

Transformer T1 with 2 different secondary coils, each connected to bridge rectifiers and voltage
regulators used for the following circuits:

1.) U1 = 5V: supplies central processor, key and indication board and temperature
measuring circuit
reference potential: connected to protective conductor (GND)

2.) U2 = 12V: supplies the circuits of speed measuring and imbalance detection
reference potential: GND2

Transformer T2 generates the low voltage supply for the power electronics:

3.) U3 = 15V: a) contacts of relay K1 (pre-loading of intermediate circuit)

- on troubles "Err. 23" will be displayed
b) and all lid switches (S2,S3,S4,S5)

- on troubles "OPEn" at standstill and "LId" during rotation will be displayed

reference potential: UD-

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Service

3.3.2 Intermediate Circuit with Brake Path and Frequency Converter

The DC intermediate circuit serves as an energy store between the AC power input and the
transmitted motor performance.
The intermediate circuit consists of a bridge rectifier and 12 parallel connected reservoir capacitors
which are softly charged at the beginning over heavy-duty resistors (R61 and R100 parallel
connected). When the capacitor's loading has reached the defined value, the resistors are
bypassed by the relay K1 to supply frequency converter with maximum main power during
operation.

Brake Path

Electrical power is fed back into the intermediate circuit during motor deceleration (motor acts as
generator). This braking power is transformed into heat by brake resistor R1 (terminal XD) so that
the intermediate circuit voltage does not rise to an excessive level (see section 2 - test points on
main board).
The brake resistor R1 is switched into the intermediate circuit by a fast switching transistor. This
transistor is voltage dependent controlled by a self-acting stage (closed loop).

Frequency Converter

The motor is 3-phased provided with chopped direct voltage blocks. These blocks are variable in
frequency and pulse width modulation and are dephased to 120° between the motor windings.
Beginning with low frequency and small pulse width chopped blocks, both parameters will be
increased up to the operators set speed. By this variation the acceleration power of the drive motor
can be effected. For deceleration the frequency and pulse width parameters will be reduced. The
frequency converter is dynamically and absolutely protected against overcurrent and overvoltage
and also absolutely protected against excessive temperature.
When exceeding one of the absolute values (overcurrent, overvoltage or excessive temperature),
the message "Err.08" will be displayed.

3.3.3 Microcontroller (Central Processing Unit)

The controller block includes the central processor unit (CPU) P87C51FC and two of the data
storage (NV-RAMs) 93S66 with the software identification No's. for:

CPU: 0599 (second displayed No. in speed field)

1. NV-RAM: 7571 (third displayed No. in speed field) 7572 in CF 8500i only

2. NV-RAM: 7573 (fourth displayed No. in speed field)

The second parts of these No.'s (displayed in the time field) will be upgraded if necessary.
The ROM with control program is integrated in the CPU. The most important operating parameters
(e.g. the maximum data or the last operator settings) are stored in the non volatile (NV) -RAM.
The CPU co-operates with the ASIC 80 component via the 8 bit data bus and some control lines.
The ASIC 80 is a specially designed integrated circuit for the driving of Kendro centrifuges (with
induction motors), containing additional functions for speed and temperature measuring and
electronics protection.

Edition: 01 Functional Description

26.12.01 MH Page: 3-4

Cryofuge 6000i/8500i

Service

3.3.4 Latch Control-system

The mainboard allows a controlling of the new electrical latch system with current software (CPU

599) and controlling of the old mechanical latch with old software (CPU 498) as well.

Note! The new Software does not control the old latch system.

Electrical latch
Both latches are connected to the board via terminal XC1 and XC2.
Each latch will be driven forward and backward via a relay by a special driver IC which changes the
polarity of the voltage depending on status of the wanted operation - open or close the door.
The activating of the door latches is several times protected by addition status controls of the CPU.
The door can only be opened if the speed is “0” and the lid opening button was pushed.

Mechanical latch
These mechanical latches are connected at the terminal XC. They are controlled by a triac which is
fired via photodiac by CPU. Two tachogenator signal trigged flip-flops prevent an opening of the
door whilst rotor is spinning.

3.3.5 Temperature measurement

The temperature will be detected by a PT 100 temperature sensor.
Two reference resistors 100 ohm = 0°C and 120 ohm = 50°C will give the reference value. The
auto-calibration is done by the CPU which controls an analogue switch where the signals will be
transferred to a voltage/frequency converter. This select signals to the switch IC will come from the
ASIC TEMP_1 and TEMP_2.

3.4 Key and Indication Board

The SEPACONTROL key and indication board is mounted behind the operational panel.
A special microcontroller (P87C51FC) is located on this board controlling the 7 segment displays,
the control key LEDs and the operating keys
The communication with the central processor on the main board takes place via serial data lines in
both directions (plug XI).
The reading of the keys and the displaying of values takes place by multiplex operation. All
operational parameters and user programs are stored unlimited in 6 NV-RAMs (type 93C46).
The constant identification No. of the key board processor is 0497 (first displayed No. in speed
field). There is a second No. displayed in the time field at the same time. This version No. is subject
of the permanently technical outgrowth and will be counted up at any program changes on CPU or
parameter changes on NV-RAMs.

3.5 Speed Detection Board

The speed detection takes place opto-electronically through a slit in the motor shaft.
A light-emitting infrared diode and a photo-transistor are mounted on the speed detection board
and located on opposing sides of the motor shaft
So 2 trapezoidal pulses per motor revolution are generated and transformed into stable rectangle
pulses (Schmitt trigger circuit on board), by which the micro controller calculates the real rotor
speed or rcf value.
The speed detection board is connected to the main board via the 3-pin plug XW.

Edition: 01 Functional Description

26.12.01 MH Page: 3-5

Cryofuge 6000i/8500i

Service

3.6 Imbalance switch

If abnormal imbalance of the rotor occurs the imbalance switch will be triggered by the moving
motor. The switch closes and this signal will be given to the CPU which detects this as
“imbalance”

The connection to the switch is made of 4-pole wiring which allows a check of the cable for
defect. In this case the imbalance signal will also be displayed.

3.7 Cooling plant

The Cryofuge is equipped with a high-grade cooling plant, to meet it’s requirements of driving
power and compact design. The CFC free refrigerant R404A evaporates at low temperatures
(≤ -20°C) and absorbs through this the frictional heat from chamber produced by the spinning
rotor. Two fans serve for even air flow through condenser so that the refrigerant’s condensing
temperature is kept on a low level.

A condenser serves for essential under-cooling of the condensed refrigerant to ensure an
optimum evaporation and a perfect operation of the expansion valve. To maintain low
evaporation and resultant condensation temperatures on one hand and to support the starting
procedure of the compressor on other hand, a thermostatic expansion valve is used.

The minimum achievable sample temperatures are depending on speed, rotor type and a little
on ambient temperature. After a compensating time (at max speed – 3 hours) as optimal result,
the rotor has reached the selected set temperature within a tolerance of ± 2K.

Edition: 01 Functional Description

26.12.01 MH Page: 3-6

Cryofuge 6000i/8500i

37 ­45°C

Verflüssiger

condenser

Kälteanlage

Cooling

Verdampferkessel

evaporator chamber

Lüfter Kühlmaschine
fans for cooling plant

2.2

J

07.05.01 MH

Sammler
accumulator

CPU

1

c.o.

>P

"E-23"

18bar
c.i.

Druck-

28bar

wächter

pressure

switch

Fließbild / Flowchart

3-7

114 - 118°C

p = 13,8 bar

-26 -

-16°C

thermostatisches Expansionsventil

R404A

Verdichter

compressor

thermostatic expansion valve

Trockner+Schauglas
Dryer+Inspectionglas

p = 1,7 bar

0

Wärmetauscher

heat exchanger

7 - 8°C

TC

-45 ­+30°C

CRYOFUGE 6000i/8500i

2.3

Netz
mains

noise filter board

3

Hauptschütz
main contactor

K2

F1

Funkentstör-

platte

3

KM-Schütz
compressor
contactor

Kühlmaschine
compressor

Kurbelwannen-

heizung

crankcase

filament

K3

Netzschalter
mains switch

M2

S1

F2

Unwucht-

schalter

imbalance

switch

S6

2 Impulse pro Motorumdrehung
2pulses per motor revolution

Drehzahler-

fassungsplatte

speed detection

board

SCHALTPLÄNE

SCHEMATIC DIAGRAMS

Schlüsselschalter
key switch

nicht belegt

not used

Temperatur-

sensor

temperature

sensor

V1

XJ XJ

Deckelschloss

Latch

Right

S2

S3

S4

Left

Motor M8Motor M7

S5

S6

S7

Tasten-und Anzeigenplatte
key and indication board

X2

RS-232

Schnittstelle

interface

X1

XI

operating panel

XZ

XZ

Bedienplatte

XY

XY

Option

NPE

L

XM1 XM2 XM3

Motor

23.04.01 MH

M1

XB

F4

Motorüber­temperatur
motor over

temperature

XT

XH

Motor/KM

Lüfter

motor/compr.

fan

M3

XE XW XL XU XA

XC 1

Hauptplatte
main board

F3

TsÜ (KM)

thermal

fuse

XN

F5

P

Pressostat

overpressure

switch

4-1

XO XP

Deckelspulen

lid solenoids

K2

Ansteuerung
Hauptschütz

mains contactor

coil

XF

FET-Lüfer

fan for

electronics

M5

XS

Lüfter Brems-

widerstand

fan of braking

resistor

M6

Blockschaltbild / Block Diagram

Y1,Y2

Old/alte

XC

fan for cooling

(entfällt bei Wasser-

*

kühlung
not used with water
cooling

Legende / legend

XC 2

KM-Lüfter

plant

M4

XK

XI

XG

K3

Ansteuerung

Kühlmaschine

compressor

coil

3x400V+N+PE

XD

Bremswider-

stand

brake resistor

R1

Steckverbindung / plug connection

Schraubverbindung / screwing connection

CRYOFUGE 6000i/8500i

Netz
mains

F1

noise filter board

3

Hauptschütz
main contactor

K2

F2

Funkentstör-

platte

F3

F4

3

KM-Schütz
compressor
contactor

Kühlmaschine
compressor

Kurbelwannen-

heizung

crankcase

filament

K3

Netzschalter
mains switch

M2

S1

Unwucht-

schalter

imbalance

switch

S6

2 Impulse pro Motorumdrehung
2pulses per motor revolution

Drehzahler-

fassungsplatte

speed detection

board

SCHALTPLÄNE

SCHEMATIC DIAGRAMS

Schlüsselschalter
key switch

nicht belegt

not used

Temperatur-

sensor

temperature

sensor

V1

XJ XJ

Deckelschloss

Latch

Right

S2

S3

S4

Left

Motor M8Motor M7

S5

S6

S7

Tasten-und Anzeigenplatte
key and indication board

X2

RS-232

Schnittstelle

interface

X1

XI

operating panel

XZ

XZ

Bedienplatte

XY

XY

Option

NPE

L

XM1 XM2 XM3

Motor

23.04.01 MH

M1

XB

F4

Motorüber­temperatur
motor over

temperature

XT

XH

Motor/KM

Lüfter

motor/compr.

fan

M3

XE XW XL XU XA

XC 1

Hauptplatte
main board

F3

TsÜ (KM)

thermal

fuse

XN

F5

P

Pressostat

overpressure

switch

4-2

XO XP

Deckelspulen

lid solenoids

K2

Ansteuerung
Hauptschütz

mains contactor

coil

XF

FET-Lüfer

fan for

electronics

M5

XS

Lüfter Brems-

widerstand

fan of braking

resistor

M6

Blockschaltbild / Block Diagram

Y1,Y2

Old/alte

XC

fan for cooling

(entfällt bei Wasser-

*

kühlung
not used with water
cooling

Legende / legend

XC 2

XK

KM-Lüfter

plant

M4

3x230V+PE

XI

XG

K3

Ansteuerung

Kühlmaschine

compressor

coil

XD

Bremswider-

stand

brake resistor

R1

Steckverbindung / plug connection

Schraubverbindung / screwing connection

CRYOFUGE 6000i/8500i