Heraeus Multifuge 1S User manual

Multifuge® 1 S / 1 S-R

Instruction Manual

How to use this manual

Use this manual to get acquainted with your centri­fuge and its accessories.

This manual helps you to avoid inappropriate han­dling. Make sure to keep it close to the centrifuge.

A manual that is not kept handy cannot provide
protection against improper handling and thus
against damage to persons and objects.

The manual comprises chapters on

• Safety regulations

• Instrument description

• Transportation and hook-up

• Rotor program and accessories

• Use of the centrifuge

• Maintenance and care

• Troubleshooting

• Technical data

• Index

Overleaf you will find a graphic

representation of the control panel

with a survey of the most important

functions

Please fold out

acceleration
profiles

program selection

"set" keys

deceleration
profiles

speed/RCF

Bucket selection

(only for instruments with refrigeration unit)

temperature

run time

rpm/RCF

Quick run

pre-temperature-regulation

switch key

display

"set" keys

Before switching on the centrifuge
please read this manual

The mains switch is located on the right-hand
side panel.

Pressed up = ON I
Pressed down = OFF 0

open lid

stop

start

Control panel of the Multifuge® 1 S / 1 S-R

Display panels

Program selection

key 1 - 4 : store or recall programs.

acceleration profiles (1= slowest ...... 9= fastest)

permanent display: acceleration profile last set 1- 9

Deceleration profiles (1= slowest ...... 9= fastest)

permanent display: deceleration profile last set 1- 9

Speed / RCF

run: current value of speed or RCF after activation of

switch key
end: "End"
lid open: "OPEN"

"Lift Lid" (if lid is not automatically lifted off)
(before start)
lid closed: "0" with flashing point
(rotor not yet identified)

error code: will flash in display

Run time

time selection: - remaining run time to 0
continuous operation (hld):

- run time passed (in hours, minutes)

quick run: - run time passed (as long as button is held;

in minutes and seconds)

Temperature*

run: current sample temperature in °C
(in temperature equilibrium)

* only for instruments with refrigeration unit

Keys

start : normal start of the centrifuge
stop : manual stop of a run
open lid: open lid

quick run: short-term operation of the centrifuge as long as

rpm/RCF
switch: switching between rpm and RCF display

bucket set.: Setting of the bucket number
pretemp : Pre-temp-function*
"set" keys: stepwise increase/decrease of setpoint values

Short pressing of any of the "set" keys: switch from current to preset
value, signaled by flashing display.

Error codes (troubleshooting see chapter "Troubleshooting"):
E-19: Unpermissible rotor
E-20: Rotor not identified
E-29: Motor or rotor blocked
E-31: Overtemperature in motor
E-32: Overtemperature in electronic
E-33: Excessive pressure in the refrigeration unit
E-34: Overvoltage
E-35: Overcurrent
Lift Lid : Smoothly lift the lid
rotor: set speed higher than permissible speed of
the rotor
bAL : imbalance

(possible only with the instrument switched on)

key remains pressed

Contents

Contents

For your safety............................................ 3

Proper use................................................................ 3

Improper use ............................................................ 3

Centrifuging hazardous materials ............................ 3

Handling the centrifuge ............................................ 4

Conformity to current standards............................... 5

Safety instructions in this manual ............................ 5

The Multifuge® 1 S / 1 S-R.......................... 7

Description ............................................................... 7

Safety systems......................................................... 8

Parts supplied .......................................................... 8

Function and features .............................................. 9

Before use................................................. 13

Centrifuge transport and installation ...................... 13

Proper location ....................................................... 13

Main connection ..................................................... 14

Rotors and accessories ........................... 15

Rotors for the Multifuge® 1 S.................................. 16

Rotors for the Multifuge® 1 S-R.............................. 20

Handling the rotor................................................... 29

Aerosol-tight operation ....................................... 32

Checking of aerosol-tight bio-containment......... 36

Operation .................................................. 37

Switching on the centrifuge .................................... 37

Actuating the lid...................................................... 37

Opening the lid ................................................... 37

Closing the lid ..................................................... 37

Installing the rotor................................................... 38

Loading the rotor .................................................... 39

Maximum loading ............................................... 39

Filling the centrifuge tubes ................................. 40

Maximum permissible load difference ................ 40

Inserting the centrifuge tubes ............................. 41

Entering parameters............................................... 43

Deceleration curves ............................................... 43

Switching from speed to RCF display .................... 43

Bucket selection for swinging bucket rotors........... 43

Selecting speed...................................................... 44

Entering the RCF value.......................................... 44

More about the RCF value ................................. 45

Selecting run time .................................................. 45

Run time selection .............................................. 45

Continuous operation ......................................... 46

Extended time mode........................................... 46

Selecting the temperature ...................................... 47

Pretemp function .................................................... 47

Starting the centrifuge ............................................ 48

1

Contents

Imbalance display................................................... 48

Changing the settings during the run ..................... 48

Stopping the centrifuge ..........................................49

Stopping with preset run time............................. 49

Stopping with continuous operation ................... 49

Temperature control during standby ...................... 49

Working with programs........................................... 50

Program display.................................................. 50

Entering/changing a program ............................. 50

Centrifuging with a program ............................... 50

”Quick Run” ............................................................51

Removing the rotor................................................. 51

Audible alarm ......................................................... 52

Turning off the centrifuge .......................................52

Maintenance and care .............................. 53

Maintenance to be performed by the customer ..... 53

Cleaning.............................................................. 53

Disinfection ......................................................... 54

Decontamination................................................. 56

Autoclaving ......................................................... 56

The KENDRO service offer .................................... 57

Warranty conditions................................................ 57

Troubleshooting .......................................59

Emergency lid release............................................ 59

Error troubleshooting.............................................. 61

Contacting Kendro Service..................................... 70

Technical Data ..........................................71

Electrical connections / fuses ................................. 73

Appendix ................................................... 75

Acceleration and deceleration profiles ................... 75

Speed/RCF diagrams............................................. 87

Index .......................................................... 93

2

For your safety

For your safety

Heraeus centrifuges are manufactured according to
current technical standards and regulations. Nonethe­less, centrifuges may pose danger to individuals and
surrounding if

• they are not used as designed

• they are operated by untrained personnel

• their design is improperly changed

• the safety instructions are not followed

Therefore, personnel involved with operation and
maintenance of the centrifuge must read and follow
the safety instructions.

In addition, the pertinent regulations for prevention of
accidents must be strictly followed.

This manual is an integral part of the centri­fuge assembly and must be kept close at
hand at all times.

When damages to the power cord or at
casing are noticed the centrifuge must
to be set out of operation!

Proper use

The centrifuge is designed to separate liquid­suspended materials having different densities and
particle size, respectively (maximum sample density is

1.2 g/cm³ {ml} at maximum speed).

Improper use

During a run, a safety zone of 30 cm around the centri­fuge must be maintained where neither persons nor
hazardous materials may be present.

The centrifuge may cause harm to its user or other
persons or may damage goods if safety measures are
not followed

:

Centrifuging hazardous materials

• The centrifuge is neither made inert, nor is it explo-

sion-proof. Therefore never use the centrifuge in an
explosion-prone environment.

• Do not centrifuge explosive or flammable sub-

stances. The same holds for substances prone to
react violently with each other.

3

For your safety

• Do not centrifuge toxic or radioactive substances or

pathogenic microorganisms without suitable safety
systems.
If microbiological samples of risk group II (according
to "Laboratory Bio-safety Manual" of WHO) are be­ing centrifuged, aerosol-tight bio-seals have to be
used.
For materials in a higher risk group, more than one
precaution is required.

• Should toxins or pathogenic substances enter the

centrifuge or its parts, you must perform appropriate
procedures for disinfection (see "Maintenance and
care – Disinfection").

• Strongly corrosive substances that may cause

damage to materials and reduce the mechanical
strength of the rotor may be centrifuged only inside
protective tubes.

Handling the centrifuge

• Use only original accessories for the centrifuge. The

only exceptions are common glass or plastic centri­fuge tubes if they are approved for the rotor speed
and RCF values.

• Never use the centrifuge unless the rotor is properly

installed.

• You may use the centrifuge only with a properly

loaded rotor. You must not overload the rotor.

• Strictly follow the rules and regulations for cleaning

and disinfection

• If the rotor or the rotor lid shows signs of corrosion

wear, you must stop using it.

or

• Never open the lid manually if the rotor is still turn-

ing.

• You may use the emergency lid release only in

case of emergency, e.g. during an interruption of
power supply
(see chapter "Troubleshooting").

• Never use the centrifuge with an opened lid.

• Never use the centrifuge if the front panel has been

partially or totally removed.

• Changes in mechanical or electrical components of

the centrifuge may only be carried out by individuals
authorized by Kendro Laboratory Products.

4

For your safety

Conformity to current standards

Heraeus centrifuges are manufactured and tested
according to the following standards and regulations:

- For all voltages

• IEC 61010

- For 120 V only

•

- For 230 V only

•

Please get acquainted with the details of the test stan­dards from the technical data.

Safety instructions in this manual

This symbol denotes potential hazards to
persons.

This symbol denotes potential damage to
the centrifuge or parts in its immediate
surroundings.

General comments are marked with this
symbol.

In addition, you are asked to adhere to the pertinent
regulations, in Germany

• Regulations for prevention of accidents BGV A2

• Regulations for prevention of accidents VBG 5

• Regulations for prevention of accidents VBG 7z

- with cooled devices additionally

• Regulations for prevention of accidents BGV D4

5

For your safety

Notes

6

Description of the Multifuge®

The Multifuge® 1 S / 1 S-R

The figure below shows a Multifuge® 1 S-R with an
opened lid and a swinging bucket rotor installed.

Description

The Multifuge® 1 S-R is a general-purpose tabletop
centrifuge for biotechnological and pharmaceutical
research that moves high capacity centrifugation onto
the fast track. It spins more tubes at higher RCFs more
rapidly than competitive instruments and can process
nominally 1.6 liters of sample in a single run. There are
various rotors available that can achieve high RCFs
and accommodate a wide range of accessories for all
common tube types, micro titer and deep well plates.

The user-friendly "EASYset" control panel permits easy
selection of speed, RCF value, run time and run profile
(acceleration and deceleration), as well as temperature
of the Multifuge
RCF display and vice versa, with a touch of a button
and even during a run.

®

1 S-R. You can switch from speed to

7

Description of the Multifuge®

Safety systems

The Multifuge® is equipped with a number of safety
systems:

• Housing and lid are constructed of 6 mm steel.

• Lid with window

• Lid lock with safety check

You can only open the centrifuge lid when the
power is turned on and the rotor has come to a
stop. You can only start the centrifuge if the lid is
properly locked.

• Automatic rotor identification

• Electronic imbalance detection as a function of rotor

(SMARTspin

TM

)

Do not tamper with the safety
systems!

Parts supplied

Accessories supplied with the centrifuge are:

− power cord

− a special wrench for securing the rotor

(As seen in the picture)

− condensed operating instructions

− corrosion protective oil

The printed documents consist of the delivery notes
and this Manual.

8

Description of the Multifuge®

Function and features

Basic unit/ function Description / feature

Cabinet / frame Galvanized steel

Chamber Stainless steel * (Multifuge 1S: coated steel)

Drive Brushless induction drive

Key pad and display Key pad and display elements covered by an easy-care continuous surface

Control Microprocessor driven by "EASYset"

Main memory Recalls last run parameters

Program memory Data are stored until new values are entered.

Advanced features RCF-programming, quick run, pre-temp *, temperature control during standby

Acceleration and deceleration pro­files

Rotor identification Automatic

SMARTspinTM
imbalance detection system

Soft touch lid lock Motor assisted lid locking

(* only with refrigeration unit)

9 acceleration and 9 deceleration profiles

Electronic, effective as a function of rotor and speed

9

Description of the Multifuge®

The EASYset user interface

Function Feature

Program memory keys Freely programmable

Acceleration / deceleration profile 1 = slowest, ... 9 = fastest acceleration / deceleration curve

Setting speed by rpm Adjustable from 300 rpm to 15 000 rpm, in 10 rpm increments

RCF selection Upon actuation of RCF switch , the RCF value can then be entered

Time selection

Run time display in "quick run" mode Between 1 s and 60 s in seconds´ steps, above in minutes´ steps

Setting temperature * Adjustable from -9°C to +40°C, in one degree increments

End of centrifugation The speed display will read "end"

(* only with refrigeration unit)

Adjustable in minutes from 1 min to 9 h 59 min,
or extended time mode from 1 min to 99 h,
”hld"-mode: continuous operation

10

Function Feature

Description of the Multifuge®

Lid opening

Start

Stop

"Quick Run" mode

Diagnostic messages

Automatic unlocking via ”open lid" key (

)

(unlocking in case of power failure: see chapter ”Troubleshooting")

Start key (

Stop key (

Pressing the "quick run" key (

)

)

) activates maximum acceleration up to the
maximum permissible speed of rotor; upon key release the centrifuge stops with
maximum deceleration power.

• Alternating display ”Rotor"/maximum speed or RCF

(speed selected exceeds max. speed of the rotor)

• Lid has not been lifted off the lock during opening:

display ”lift lid" (manual lifting of lid is required)

• General instrument malfunction

(error messages with ERROR codes, see "Troubleshooting")

11

Description of the Multifuge®

Notes

12

Before use

Centrifuge transport and installation

After opening the box remove the protective materials.

When transporting the centrifuge,
consider its weight (see "Technical
Data"); always grab it on both sides
ensuring that enough helpers are
around.

Do not lift on the front panel!

Before use

The centrifuge can be damaged by
jolting during the transport!

Transport the centrifuge only in the
upright position using proper con­tainment and secure it properly.
Handle the centrifuge carefully.

Proper location

The centrifuge may only be used indoors. Its location
must meet the following criteria:

• A safety zone of at least 30 cm (12 inches) around

the centrifuge must be maintained. Hazardous ma­terials must not be stored beside the centrifuge dur­ing its use.

• The laboratory bench or centrifuge trolley must be

stable and resonance-free. A good support is pro­vided by a laboratory bench or a centrifuge cart with
lockable casters.

• To ensure sufficient air circulation, a minimum dis-

tance from the wall of 10 cm (4 inches) at the back
and of 15 cm (6 inches) on each side must be kept.

13

Before use

• The centrifuge must be protected from heat and

direct sunlight.

• The location must be well ventilated at all times.

UV rays reduce the durability of
plastics.

Protect the centrifuge, rotors and
plastic accessories from direct
sunlight.

Main connection

Connect the centrifuge only to a grounded main power
supply. Make sure that the power cord is compatible
with the valid safety regulations and that your main
voltage and frequency correspond to the specifications
on the instrument label.

Make sure that the centrifuge is switched off (on the
right-hand side of front panel) before connecting
electrical wire to main power supply.

RS232 interface

specifications

Main connection

14

Rotors and accessories

Rotors and accessories

A rotor is not included as part of a Multifuge® centri­fuge.

A large variety of rotors are available as accessories.
In addition, there are adapters and reduction sleeves

for a variety of commercially available tubes and
bottles.

For a complete list of available tubes, bottles, adapt­ers and accessories, please refer to our current sales
documentation.

For more information you can visit our web site at
http://www.Kendro.com

Multifuge

During centrifugation, heat is generated by air friction
between the rapidly spinning rotor and the air inside
the rotor chamber. The continuous air flow in the air
cooled centrifuge is restricting the temperature rise of
the samples. The equilibrium temperature rise de­pends on the ambient temperature, the type of rotor,
the speed, the duration and the number of runs.

®

1 S (air cooled)

Use caution when touching rotors as
they may be hot after long runs at
high speed.

15

Rotors and accessories

Rotors for the Multifuge® 1 S

Table 1: Rotors for Multifuge

Rotor designation Swinging Bucket Rotor TTH 400

®

1 S

Differences of 120V instruments are shown in parentheses

75002000

With bucket

Order no.

Maximum permissible load [ g ] 4 x 540 4 x 570

Maximum speed nmax [ rpm ] 4700 ( 4500 ) 4700

Maximum RCF value at nmax 4618 ( 4234 ) 4618

Radius (max.) [ cm ] 18.7 18.7

Acceleration / deceleration time [ s ] 40 / 35 ( 50 / 40 ) 40 / 35 ( 55 / 40 )

Heating of samples at nmax [ °C ]
relative to room temperature 23°C,

run time 1 hour

Aerosol-tight * Yes (with cap 75002003) Yes (with cap 75002004)

Autoclavable 121°C 121°C

* Tested and approved by CAMR, Porton-Down, UK

12.0 ( 14.0 )

Rectangular bucket

150 ml

75002001

13.0

Round bucket

400 ml

75002002

16

Table 1: Rotors for Multifuge

Rotor designation Swinging Bucket Rotor TTH 400

®

1 S

Differences of 120V instruments are shown in parentheses

75002000

Rotors and accessories

With bucket

Order no.

Multiple carriers

14 x 15 ml

75002027

Multiple carriers

5 x 50 ml + 2 x 15 ml

75002028

Maximum permissible load [ g ] 4 x 420 4 x 430

Maximum speed nmax [ rpm ] 4700 ( 4500 ) 4700 ( 4500 )

Maximum RCF value at nmax 4642 ( 4256 ) 4593 ( 4210 )

Radius (max.) [ cm ] 18.8 18.6

Acceleration / deceleration time [ s ] 40 / 35 ( 65 / 40 ) 40 / 35 ( 65 / 40 )

Heating of samples at nmax [ °C ]

relative to room temperature 23°C,

run time 1 hour

15.0 ( 13.0 )

15.0 ( 13.0 )

Aerosol-tight — —

Autoclavable 121°C 121°C

17

Rotors and accessories

Table 1: Rotors for Multifuge

Rotor designation

Order no.

Maximum permissible load [ g ] 4 x 540 2 x 600

Maximum speed nmax [ rpm ] 5700 4400

Maximum RCF value at nmax 6175 3355

Radius (max.) [ cm ] 17.0 15,5

Acceleration / deceleration time [ s ] 55 / 55 ( 80 / 50 ) 35 / 35 ( 40 / 35 )

®

1 S Differences of 120V instruments are shown in parentheses

®

BIOshield

600 Rotor

4 x 150 ml

75002005

Micro plate carrier

MP 3300
75002010

Heating of samples at nmax [ °C ]

relative to room temperature 23°C,

Run time 1 hour

11.0

8.0

Aerosol-tight * Yes Yes (with cap 75002011)

Autoclavable 121°C 121°C

* Tested and approved by CAMR, Porton-Down, UK

18

Rotors and accessories

Table 1: Rotors for Multifuge

Rotor designation

Order no.

Maximum permissible load [ g ] 6 x 140 / 12 x 30 48 x 4

Maximum speed nmax [ rpm ] 10 150 15 000

Maximum RCF value at nmax 14 513 24 652 - outer ring

Radius (max. / min.) [ cm ] 12.5 / 6.1 9.8 / 5.9

Tube angle [ ° ] 45 45

Acceleration / deceleration time [ s ] 40 / 50 ( 60 / 45 ) 35 / 30 ( 40 / 35 )

®

1 S Differences of 120V instruments are shown in parentheses

Fixed-Angle Rotor FA12.94 Highconic

6 x 94 ml / 12 x 16 ml

75002006

®

Micro Liter Rotor

48 x 2 ml

75003348

21 885 - inner ring

Heating of samples
[ °C / at rpm / run time ]

relative to room temperature 23°C

9.0 °C / 10 150 / 1 h

15.0 °C / 12 000 / 1 h

15.0 °C / 15 000 / 15 min

23.0 °C / 15 000 / 1 h

Aerosol-tight * Yes Yes

Autoclavable 121° 138°

* Tested and approved by CAMR, Porton-Down, UK

19

Rotors and accessories

Rotors for the Multifuge® 1 S-R

Table 2: Rotors for Multifuge

Rotor designation Swinging Bucket Rotor TTH 400

®

1 S-R

Differences of 120V instruments are shown in parentheses

75002000

With bucket

Order no.

Maximum permissible load [ g ] 4 x 540 4 x 570

Maximum speed nmax [ rpm ] 4700 ( 4600 ) 4700

Maximum RCF value at nmax 4618 ( 4424 ) 4618

Radius (max.) [ cm ] 18.7 18.7

Acceleration / deceleration time [ s ] 35 / 35 ( 45 / 40 ) 35 / 35 ( 45 / 40 )

Min temperature at nmax [ °C ]

relative to room temperature 23°C

Speed at 4°C [ rpm ] 4300 ( 4100 ) 4700 ( 4450 )

Aerosol-tight * Yes (with cap 75002003) Yes (with cap 75002004)

Autoclavable 121°C 121°C

* Tested and approved by CAMR, Porton-Down, UK

10 ( 12 ) 4 ( 7 )

Rectangular bucket

150 ml

75002001

Round bucket

400 ml

75002002

20

Table 2: Rotors for Multifuge

Rotor designation Swinging Bucket Rotor TTH 400

®

1 S-R

Differences of 120V instruments are shown in parentheses

75002000

Rotors and accessories

With bucket

Order no.

Multiple carriers

14 x 15 ml

75002027

Multiple carriers

5 x 50 ml + 2 x 15 ml

75002028

Maximum permissible load [ g ] 4 x 420 4 x 430

Maximum speed nmax [ rpm ] 4700 ( 4500 ) 4700 ( 4500 )

Maximum RCF value at nmax 4642 ( 4256 ) 4593 ( 4210 )

Radius (max.) [ cm ] 18.8 18.6

Acceleration / deceleration time [ s ] 40 / 35 ( 65 / 40 ) 40 / 35 ( 65 / 40 )

Heating of samples at nmax [ °C ]

relative to room temperature 23°C,

run time 1 hour

15.0 ( 13.0 )

15.0 ( 13.0 )

Aerosol-tight — —

Autoclavable 121°C 121°C

21

Rotors and accessories

Table 2: Rotors for Multifuge

Rotor designation

Order no.

Maximum permissible load [ g ] 4 x 540 2 x 600

Maximum speed nmax [ rpm ] 6000 4400

Maximum RCF value at nmax 6842 3355

Radius (max.) [ cm ] 17,0 15,5

Acceleration / deceleration time [ s ] 50 / 50 ( 70 / 55 ) 35 / 40 ( 40 / 40 )

®

1 S-R Differences of 120V instruments are shown in parentheses

®

BIOshield

600 Rotor

4 x 150 ml

75002005

Micro plate carrier

MP 3300

75002010

Min temperature at nmax [ °C ]

relative to room temperature 23°C

5 ( 8 ) < 0

Speed at 4°C [ rpm ] 5900 ( 5700 ) 4400

Aerosol-tight * Yes Yes (with cap 75002011)

Autoclavable 121°C 121°C

* Tested and approved by CAMR, Porton-Down, UK

22

Rotors and accessories

Table 2: Rotors for Multifuge

Rotor designation

Order no.

Maximum permissible load [ g ] 6 x 140 / 12 x 30 48 x 4

Maximum speed nmax [ rpm ] 10 350 15 000

Maximum RCF value at nmax 15 090 24 652 - outer ring

Radius (max./min.) [ cm ] 12.5 / 6.1 9.8 / 5.9

Tube angle [ ° ] 45 45

Acceleration / deceleration time [ s ] 45 / 45 ( 55 / 50 ) 30 / 30 ( 40 / 30 )

®

1 S-R Differences of 120V instruments are shown in parentheses

Fixed-Angle Rotor FA12.94 Highconic

6 x 94 ml / 12 x 16 ml

75002006

®

Micro Liter Rotor

48 x 2.0 ml

75003348

21 885 - inner ring

Min temperature at nmax [ °C ]

relative to room temperature 23°C

0 ( 4 ) 4 ( 6 )

Speed at 4°C [ rpm ] 10 350 15 000 ( 14 500 )

Aerosol-tight * Yes Yes

Autoclavable 121°C 138°C

* Tested and approved by CAMR, Porton-Down, UK

23

Rotors and accessories

Adapter

Table 3: Adapter (1)

Adapter and accessories for
rectangular buckets 7500 2001

Centri-Lab® Adapter type A plus

14 x 7 ml blood sampling 13.0 x 117 16.5 56 grey 7500 2022
12 x 15 ml blood sampling 17.0 x 117 19.5 48 white 7500 2021
8 x 15 ml conical 17.0 x 120 22.0 32 brown 7500 2020

Centri-Lab® Adapter type A
40 x 1.5 / 2 ml micro liter tube 11.0 x 45 - 160 black 7500 5335
20 x 7 ml DIN 13.0 x 115 13.0 80 yellow 7500 5321
12 x 15 ml DIN 17.0 x 115 18.3 48 red 7500 5322
4 x 25 ml universal container 22.5 x 100 31.0 16 green 7500 5391
5 x 25 ml DIN 25.0 x 115 25.9 20 orange 7500 5323
2 x 50 ml DIN 34.0 x 120 35.6 8 green 7500 5324
2 x 50 ml conical 29.5 x 120 37.5 8 green-yellow 7500 5386
1 x 50 ml oil test - - 4 nature 7500 5339
1 x 100 ml DIN 44.0 x 120 48.1 4 blue 7500 5325
1 x 150 ml DIN 50.0 x 110 - 4 grey-blue 7500 5326
for cyto-system - - - - 7600 3417

Aerosol-tight caps

Spar seal

1)

d = diameter

* Max. tube length with aerosol-tight cap

Max. tube

dimensions

d1) x length/ *

[mm]

2 pieces, incl. seals and lubricants

4 pieces, incl. lubricants

Cap

diameter

[ mm ]

Tubes
per
rotor

Color

Order no.

7500 2003

7500 2008

24

Rotors and accessories

Table 3: Adapter (2) * Max. tube length with aerosol-tight cap

Cap

diameter

[mm]

Adapter and accessories for
round buckets 7500 2002

Max. tube

dimensions

1)

d

x length / *

[mm]

Centri-Lab® Adapter type E

34 x 1.5 / 2 ml micro liter tube 10.5 x 46 14.0 136 black 7500 7578
18 x 7 ml DIN 13.0 x 123 14.0 72 yellow 7500 7571
10 x 7 ml blood sampling 13.0 x 123 18.4 40 grey 7500 7579
10 x 15 ml DIN 17.2 x 122 18.4 40 red 7500 7572
8 x 15 ml conical 17.0 x 127 23.0 32 brown 7500 7621
5 x 25 ml US-urine 16.5 x 123 27.5 20 orange-red 7500 7581
5 x 25 ml DIN 25.5 x 121 29.0 20 orange 7500 7573
3 x 50 ml universal container 25.0 x 120 32.0 12 green-yellow 7500 7580
3 x 50 ml conical 29.0 x 119 37.5 12 green-yellow 7500 7577
3 x 50 ml DIN 34.5 x 120 36.0 12 green 7500 7574
1 x 100 ml DIN 45.0 x 134 59.0 4 blue 7500 7575
1 x 250 ml bottle 62.0 x 135

400 ml bottle
Aerosol-tight caps

Spar seal

1)

d = diameter

x 135

80.0

2 pieces, incl. seals and lubricants

4 pieces, incl. lubricants

-

-

Tubes

Color

No. of order
per
rotor

4 nature 7500 7622
4

nature 7500 7583

7500 2004

7500 2009

25

Rotors and accessories

Table 3: Adapter (3) * max. tube length with aerosol-tight cap

Cap

diameter

[ mm ]

Adapter and accessories for
BIOshield

®

600 Rotor 7500 2005

Max. tube

dimensions

1)

d

x length / *

[mm]

Centri-Lab® Adapter type A
40 x 1.5 / 2 ml micro liter tube 11.0 x 45 - 160 black 7500 5335
20 x 7 ml DIN 13.0 x 115 13.0 80 yellow 7500 5321
12 x 7 ml blood sampling 13.0 x 117 16.5 48 grey 7500 5330
11 x 15 ml blood sampling 17.0 x 117 19.5 44 white 7500 5327
6 x 15 ml conical 17.0 x 120 22.0 24 brown 7500 5387
12 x 15 ml DIN 17.0 x 115 18.3 48 red 7500 5322
4 x 25 ml universal container 22.5 x 100 31.0 16 green 7500 5391
5 x 25 ml DIN 25.0 x 115 25.9 20 orange 7500 5323
2 x 50 ml DIN 34.0 x 120 35.6 8 green 7500 5324
2 x 50 ml conical 29.5 x 120 37.5 8 green-yellow 7500 5386
1 x 50 ml oil test - - 4 nature 7500 5339
1 x 100 ml DIN 44.0 x 120 48.1 4 blue 7500 5325
1 x 150 ml DIN 50.0 x 110 - 4 black 7500 5326
for cyto-system - - - - 7600 3417

Spar seal

1)

=

1 set, incl. lubricants

Tubes
per
rotor

Color

No. of order

7500 2007

26

Table 3: Adapter (4)

Rotors and accessories

Adapter and accessories for
Fixed-Angle Rotor FA12.94
7500 2006

Adapter for 94 ml cavity

1.5 ml micro tube 11 x 58 4 24 nature 7600 2905

3.5 ml 11 x 103 4 24 nature 7500 3091

6.5 ml 13 x 115 2 12 nature 7500 3092
12 ml 16 x 96 2 12 nature 7500 3093
16 ml * 18 x 124 1 6 nature 7600 2906
38 ml 25 x 112 1 6 nature 7500 3094
50 ml 29 x 118 1 6 blue 7500 3102
15 ml conical 16.5 x 120 1 6 nature 7500 3095
50 ml conical 30 x 117 1 6 green 7500 3103

Spare seal

Adapter for 16 ml cavity

for 10 ml Vacutainer Reducer insert (16 x 100 mm) 7500 3763
for 7 ml Vacutainer *

for 5 ml Vacutainer

1)

d = diameter

Max. tube

dimensions

1)

d

x length

[mm]

2 sets, incl. lubricants

Reducer insert (13 x 100 mm)
Reducer insert (13 x 75 mm)

Number per
adapter

Number
per
rotor

Color No. of

order

7500 3058

7600 3225

7600 3226

* For 7 ml Vacutainer, Adapter 7600 2906 and 7600 3225 required.

27

Rotors and accessories

Tabelle 3: Adapter (5)

Accessories for
Micro plate carrier MP 3300
7500 2010

Aerosol-tight cap
Adapter for micro plate carrier
Spare seal

Table 3: Adapter (6)

2 pieces, incl. seals and lubricants

2 pieces, incl. lubricants

No. of

order

7500 2011
7500 2013
7500 2012

Max. tube

Adapter for
Micro Liter Rotor 7500 3348

Reduction sleeve PCR 6.2 x 20 0,2 24 Grey 7600 3750
Reduction sleeve 8 x 43.5 0.5 / 0,6 24 Turquoise 7600 3758

Reduction sleeve 6 x 46 0.25 / 0.4 24 Red 7600 3759

1)

d = diameter

28

dimensions
d1) x length

[ mm ]

Tube capacity

[ ml ]

Number
per set

Color No. of

order

Handling the rotor

Rotors and accessories

Swinging Bucket Rotor TTH 400
BIOshield

®

600 Rotor

Micro plate carrier

All positions must always be loaded
with identical carrier buckets!

The various swinging buckets are split up into weight
categories. These can be identified through the letters
suffixing the order number on the bucket. Buckets of
identical weight categories should always be installed
in opposing rotor positions to avoid imbalance.

In case of repeat orders of buckets
and carriers, please indicate the
present weight category.

The swinging bucket rotors have a slide coating which
guarantees perfect operation without additional lubrica­tion of body trunnion pins for many years.

Should an imbalanced run occur although
the weight is correct, this could be due to
wear of the slide coating.

In this case the rotor function remains intact
through normal lubrication:
at regular intervals, apply a light coating of
lubricant to the rotor body trunnion pins and
to the corresponding mating surfaces of the
buckets!

Lubricant 7000 6692 is supplied with the
centrifuge.

29

Rotors and accessories

BIOshield

®

600 Rotor 7500 2005

Do not run the rotor without the rotor
cover installed.

Store the BIOshield

®

Rotor with the cover removed
after cleaning. This will enable the rotor to dry
thoroughly.

Always maintain the rotor in the recom­mended manner.

The rotor and accessories must be cleaned
and inspected regularly: do not use when
showing signs of corrosion or cracking.

30

Rotors and accessories

Micro plate carrier MP 3300 7500 2010

Remove the appropriate plate holder from
the carrier body for loading and unloading of
micro plates in the Micro plate carrier.

Before loading, ensure that the rubber bottom is placed
in the cut-outs of the bottom of the plate holder.

The maximum loading height amounts to 55 mm!

Make sure the rotor is balanced!

The operation is permissible only
with the associated carriers!

(Label "75002010")

Some commercially available micro­plates are not rated for the maximum
achievable RCF of the Microplate car­rier. Therefore please pay attention to
the specifications of the plate manu­facturer.

31

Rotors and accessories

Aerosol-tight operation

Aerosol-tight rotors and tubes are only
to be opened in an approved safety
work bench when centrifuging danger­ous samples!

It is necessary to pay attention to the
maximum permissible filling quantities!

Correct operation when filling the sample tubes and
closing the rotor cover are prerequisites for aerosol bio­containment.

Before use, the seals in the rotors and
rotor covers as well as the aerosol-tight
caps have to be checked for abrasion
or damage and have to be slightly
greased.

Replace damaged O-rings and seals!

Use the special lubricant
7600 3500 only to grease the seals!

Spare parts are delivered with the rotor or may be or­dered separately.

Replace damaged or clouded caps
and lids of rotors and tubes
immediately.

The tubes are only to be filled in such a way that the
sample does not reach the rim of the tube during cen­trifuging.

32

Rotors and accessories

Closing the rectangular bucket 75002001
aerosol-tightly

After putting on the cap both levers are pressed down
until they noticeably click in.

Both of the levers must click in order to
achieve aerosol-tight bio-containment!

Closing the Micro test plates rotor MP3300
75002010
aerosol-tightly

Please flap both lock levers upward. The cap can now
be easily put on the bucket. The bucket is locked
through flapping down the levers.

Both of the levers must click in order to
achieve aerosol-tight bio-containment!

Levers not being flapped down
may cause damage to the caps
during centrifugation!

33

Rotors and accessories

Closing the round bucket 75002002
aerosol-tightly

After greasing the seal, turn the lid until it sits lightly on
the bucket.

To achieve uniform seal, turn the lid tighter by 1 grip
area (approx. 10°). Use the setting marks on the
bucket as a guide.

Grip area

Bucket mark

Closing Fixed-Angle Rotor aerosol-tightly

The hexagon wrench should be used as a support tool
to fasten and loosen the lid of the fixed angle rotor in
order to achieve secure closing (insert the hexagon
wrench through the hole in the screw cap).

Please pay attention to the maximum
permissible filling volume during cen­trifugation of dangerous samples!

34

Rotors and accessories

Rotor

Vessel type /

maximum filling volume

Micro Liter Rotor 48 x 2.0 ml
75003348

Fixed-Angle Rotor FA12.94
75002006

Micro 1,5 ml

1,0 ml

Falcon 50 ml

49 ml

Micro 2,0 ml

1,5 ml

Falcon 15 ml

14 ml

others:

- 2/3 nominal volume

• Micro – micro centrifuge tube

• Falcon – tube type Falcon

35

Rotors and accessories

Checking of aerosol-tight bio-containment

The checking of the rotor type and bucket was done
according to the dynamic microbiological test proce­dure with regard to EN 61010-2-020 appendix AA.

The aerosol-tight bio-containment of the rotor mainly
depends on proper handling!

Check the aerosol-tight bio-containment
of your rotor whenever necessary!

It is very important that all the seals and
seal-surfaces are carefully inspected for
wear and damages like cracks, scratches
and embrittlements!

For a quick test one can check the aerosol-tight buck­ets and fixed angle rotors according to the following
procedure:

• Slightly grease all seals.

• Fill the bucket or rotor with approx. 50 ml carbon

dioxide mineral water.

• Close the bucket or rotor according to the respec-

tive handling instructions.

• Shaking the bucket releases the carbon dioxide of

the water, and an excessive pressure is built up.

• Leaks are recognized by humidity release and

audible disinflation of gas mix.

• Finally buckets respectively rotor, lid and lid seal

have to be dried.

36

Operation

Switching on the centrifuge

Locate the main power switch on the right-hand side of
the front panel, and set it to the ON (I) position. For a
couple of seconds the following reading appears in the
control panel:

The display shows that the instrument is going through
an internal check of its software.
(See table on page 70).

After this check, the display shows the actual value
mode. The remaining run time and speed should both
read 0. The display of the acceleration/deceleration
curve depends on the last set value.

The following figure gives an example of possible read­ings. A detailed description of possible settings is given
below in this chapter.

Operation

Actuating the lid

Opening the lid

Press the "open lid" key
If the message "Lift lid” appears, slightly lift the lid.
(Emergency release in case of malfunction or power

failure: see chapter "Troubleshooting")

Closing the lid

The centrifuge lid is locked by slightly pressing down
the front part of the lid. Locking is motor-driven.

Do not slam the lid!

.

37

Operation

Installing the rotor

Improper or improperly combined
accessories may cause severe dam­age to the centrifuge!

The rotors approved for the Multifuge® are detailed in
the chapter "Rotors and accessories". Use only rotors
listed for this instrument.

To install the rotor
ter "Parts supplied” – page 8).

Proceed as follows:

1. Open the lid and make sure that the rotor chamber

is clean. Clean any dust, foreign material or sample
residues out of the chamber before use.

2. Check if the collet chuck is loose (collet chuck

moves freely on the spindle). If not, loosen the rotor
seat using the supplied socket wrench.

3. Place the rotor on top of the collet chuck so that the

rotor is located precisely above the center.

, you will need the wrench (see chap-

4. The rotor must glide freely down the collet chuck
until it hits the lower stop.

5. If you have positioned the rotor correctly, you can
tighten the collet chuck easily using the supplied
hex wrench.

6. Place the rotor cover on applicable rotors and
tighten it securely.

Regularly check the proper positioning
of the rotor and re-tighten the collet
chuck as needed.

Please take care of the legibility of the inscription of the
installed swinging bucket rotor cross.

(Rotor identification must point to the chamber bottom)
For the swinging bucket rotor, the set bucket type must

be permitted for the operation in the respective rotor.
Please note the requirements of chapter "Bucket selec­tion of swinging bucket rotors"

38

Operation

Loading the rotor

Maximum loading

Overloading can result in destruction
and severe damage to the centrifuge.

The Multifuge
ing enormous centrifugal force. The rotors are de­signed to warrant sufficient residual strength even at
the highest permissible speed.

However, this safety system presupposes that the
maximum permissible load of the rotor is not exceeded.

®

can reach high rotational speeds exert-

Please get acquainted with the data
about the maximum permissible loads
and maximum speeds in chapter "Ro­tor and accessories".

If you wish to centrifuge samples that, together with the
adapters, exceed the maximum permissible load, you
must either reduce the sample volume or calculate the
permissible speed n
mula:

n

* n
n

perm

n= ∗

max

= permissible speed

perm

= maximum speed

max

according to the following for-

perm

maximum permissible load

actual load

39

Operation

Filling the centrifuge tubes

Check carefully whether your tubes
are approved for the respective RCF
value. Follow tube manufacturer’s
recommendation.

For common borosilicate glass tubes the maximum
permissible rcf is limited to 4000 xg!

The tube manufacturers normally limit the respective
maximum allowed RCF value to the fixed angle rotor.

Please note that for the same RCF value
the stress for the tubes in a swinging­bucket rotor is higher!

Because of the higher difference of the radii (r

max

- r

min

)
the pressure of liquid column to the tube bottom is
appreciably higher and strongly depended on filling.

Plastic tubes and bottles – especially for the highest
load (speed, temperature) – have a limited life time and
must be replaced as recommended by the manufac­turer.

Maximum permissible load difference

The smaller the imbalance of the centrifuge,
the better the separation effect, because as
imbalance is minimized, so is the resulting
vibration, which could affect separation
quality.

Therefore it is important that the tubes are properly
balanced.

In case of exceeding the rotor specific imbalance val­ues, the electronic imbalance shut-down is activated.

40

Operation

Inserting the centrifuge tubes

The rotor must be loaded symmetri­cally. Failure to do so can cause rotor
imbalance, which may lead to noisy
operation, affect separation quality, or
result in imbalance detection shut­down, as well as introduce significant
detrimental wear to the motor and
drive system.

When only partially loading the rotor, ensure that oppo­site bores receive tubes of equal weight (when centri­fuging a single sample, place a centrifuge tube filled
with water opposite of the sample).

After placing the tubes, install the rotor cover.

Fixed-angle rotors:

Proper loading

Improper loading

41

Operation

Swinging bucket rotors:

Proper loading

Improper loading

These examples are to be applied to the
other rotors in an analogous manner!

42

Operation

Entering parameters

Deceleration curves

The Multifuge® offers 9 acceleration and deceleration
profiles for optimal centrifuging of samples and gradi­ents. Please consult the diagrams in the Appendix for
more detail of the acceleration and deceleration curves
(for rotors not mentioned here you may extrapolate the
respective values).

After switching on the centrifuge, the centrifugation
profiles last entered are selected.

By pressing the "set" key
profile settings until the desired profile is reached.

Once the display stops flashing, the value is stored in
memory and remains unchanged until changed by a
new entry.

Switching from speed to RCF display

Upon turning on the centrifuge, the speed display is
set.

Use the speed mode selection key
speed entry and display between rpm and RCF.

you can scroll through the

to switch the

Bucket selection for swinging bucket
rotors

When running a swinging bucket rotor, the automatic
rotor identification feature will recognize the rotor body.
Depending on the bucket or carrier installed on the
rotor body the corresponding type of bucket or carrier
must be selected on the bucket selection function. The
bucket selection affects the correct RCF values display
and the correct selection of the corresponding parame­ters of the temperature regulation.

The current part number of the buckets is displayed by
pressing the bucket selection key (corresponds to the
last four 4 digits of the order number).

To change the bucket
selection, press the
bucket selection key
again until the correct
set of buckets appears.

The value is accepted when the change back to the
speed RCF display has occurred.

43

Operation

Selecting speed

The centrifuge speed can be set to a minimum of 300
rpm and to a maximum of 15 000 rpm (depending on
the rotor).

You can adjust the speed in 10 rpm increments. Pro­ceed as follows:

1. By pressing the "set" keys

or

(for a decrease) in the "speed" section of the
control panel, you switch from actual to set point
values. The value last stored is displayed, with the
digit entered flashing (if there is no value stored in
memory, this is indicated by dashes -----).

2. By briefly pressing the input key you can raise or

3. If you hold down the pressed key, the display
changes slowly at first and at an accelerated pace
after a few seconds.

4. Release the key as soon as you have reached the
desired value, and adjust if necessary by repeatedly
pressing the key. The decimal place flashes for a

once (for an increase)

lower the speed by
one step (10 rpm)
at a time.

number of seconds, then changes to a permanent
display. The speed is now stored.

For faster operation, you may shift the flash­ing cursor in the speed/RCF and the run
time panels: just press both

and si­multaneously. The cursor moves by one
digit to the left for each time the key is
pressed.

Entering the RCF value

You can adjust the RCF set point in steps of 1. The set
point is entered analogously to the speed.

As long as the rotor has not been identified, it is not
possible to display RCF values. This is signaled by
dashes ----- in the display.

Shortly after starting the centrifuge run, the rotor is
identified and the current value is displayed.

NOTE:
If you set an extremely low RCF value, this may be

automatically corrected if the resulting speed would be
lower than 300 rpm.

44

Operation

More about the RCF value

The relative centrifugal force (RCF) is given in multi­ples of the earth gravity g. It is a dimensionless number
that allows comparing the efficiency of separation or
sedimentation of diverse instruments since it is inde­pendent of the instrument used. The only values en­tered in the equation are radius and speed of centrifu­gation

:



RCF ∗

r = radius of centrifugation in cm
n = speed in rpm

The maximum RCF value refers to the maximum ra­dius of the tube bore.

You may take this into account when calculating the
RCF value for your application.

18,11

∗=



1000



Please note that this value decreases
depending on the tubes and adapters used.

2

n



r




Selecting run time

There are two time modes: standard and extended.
In the standard time mode you can select a run time

between 1 min and 9 h 59 min or continuous operation
(hLd) .

In the extended mode you can select a run time be­tween 1 min and 99 h (from 10 h in one-hour steps), or
continuous operation (hLd).

Run time selection

To set a time, proceed as follows:

1. Press one of the "set" keys
(for a decrease) in the "run time" section of the

control panel once to switch from the actual to the
set point mode.

2. By briefly pressing the "set” key you can now

(for an increase) or

raise or lower the run
time in 1-minute incre­ments.

45

Operation

3 If you keep the selected key pressed, the dis-

play changes slowly at first and at an acceler­ated pace after a few seconds.

4 Release the key as soon as you have reached

the desired value, and adjust if necessary by
repeatedly pressing the key.

The minute display flashes for a number of
seconds, then changes to permanent display.
The run time is now stored.

You may shift the flashing cursor to set the value as
described under "Selecting speed".

Continuous operation

To switch the Multifuge
must press the key

®

to the continuous mode, you

until the display reads "hLd") .

With this

setting, the centri­fuge keeps running until stopped
manually.

Extended time mode

You have the option of switching to the extended time
mode. To switch it on or off press the program selec­tion key

and simultaneously start the centrifuge. As long as
you keep the key pressed the selective mode is active.

Pressing the upward key

, you can switch back and
forth between the signal menu "beep" and the time
menu "t-set".

After selecting the time menu "t-set”, you can switch
between the standards time mode "00.0" and the ex­tended time mode "00”.h by pressing the upward key

.

The time input in excess of 10 hours is set in one hour
increments.

46

Operation

Selecting the temperature

You can select the temperature in the range of -9 °C to
+40 °C.

To adjust the temperature, proceed as follows:

1. Press one of the "set" keys
(for a decrease) in the "temperature" section of

the control panel once to switch from the actual to
the set point mode.

2. By briefly pressing the input key you can now raise

or lower the run time in 1°
steps.

3. If you keep the selected key pressed, the display

changes continuously slowly at first and then at ac­celerated paces up or down.

4. Release the key as soon as you have reached the

desired value, and adjust if necessary by repeatedly
pressing the key.

The temperature display flashes for a number of sec­onds, then changes to the current value display. The
temperature set point is now stored.

(for an increase) or

Pretemp function

The Pretemp function permits easy and quick pre­temperature-regulation of the empty rotor.

Upon calling this function by pressing the key
you have to do is enter the desired temperature.

After actuating the start key

, the rotor is pre­tempered with an optimum rotational speed set by the
pre-temperature-function.

The actually achieved temperature change in the pre­temperature run time depends on the rotor used and
the ambient temperature. Occasionally multiple pre­temperature runs are necessary to achieve the desired
rotor temperature.

, all

47

Operation

Starting the centrifuge

Once the rotor is properly installed, the main switch is
turned on and the lid is closed, you can start the centri­fuge.

Press the "start" key
fuge accelerates to the selected value. Simultaneously,
the time display starts counting down from the selected
set time, giving the remaining run time in minutes (dur­ing continuous operation the time display goes for­ward).

If a value exceeding the maximum permissible speed
or RCF of the respective rotor was entered, this is indi­cated after the start of the centrifuge by the alternately
flashing messages "rotor" and the maximum permissi­ble value for the inserted rotor.

Within 15 seconds you may adopt this value by again
pressing the "start" key; the centrifugation is then con­tinued. Otherwise the centrifuge stops, and you must
enter a permissible value.

You cannot open the lid during the run.

in the control panel. The centri-

Imbalance display

If rotor imbalance is detected, shortly after the rotor
reaches 300 rpm, the message "bAL" will appear in the
speed display.

The run is terminated,
and you may restart the
centrifuge after correct­ing the imbalance (check
loading).

Changing the settings during the run

You can change all settings during a run. By pressing
any one of the "set" keys in the control panel once, you
can switch from the actual to the set point mode.

The to-be-adjusted setting flashes and can then be
altered. Once the data input is finished and the display
has changed to the actual value display mode, the new
settings become operative.

48

Operation

Stopping the centrifuge

Stopping with preset run time

Normally the run time has been selected, and all you
have to do is to wait until the centrifuge terminates the
run automatically at the end of the set time.

As soon as the speed reaches zero, the display reads
"end". You can now open the centrifuge by pressing
the "open lid" key

If the lid has not been fully lifted out of the lid lock, the
message "lift lid" appears (you must manually lift the
lid).

You can manually stop the centrifuge at any time by
pressing the "stop" key

At this point the remaining run time is displayed.

Stopping with continuous operation

If you have chosen continuous operation, you must
stop the centrifuge manually. Press the "stop" key
the control panel. The centrifuge starts deceleration
with the preset deceleration profile.

The display reads "end", and you can open the lid by
pressing the "open lid" key
ples.

and remove your samples.

.

in

and remove your sam-

Temperature control during standby

Temperature control becomes active once the rotor
has been identified. This is the case after a centrifuga­tion run exceeding 300 rpm. At standby the display
reads "end".

If the rotor has not been identified (lid has been closed
and the "start" key
speed panel shows "0" with flashing point), the instru­ment regulates the temperature so that the samples
cannot freeze in any one of the usable rotors.

If you find the systematic deviation in temperature of up
to 4 K unacceptable, you have to start the centrifuge
for a short period of time until the rotor is identified.

has not yet been pressed,

49

Operation

Working with programs

The 4 program selection keys offer the option of storing
and recalling the individual centrifugation processes.

Program display

By actuating one of the program selection keys, the
specific set point values stored are displayed.

Entering/changing a program

All program places have been factory-set at the same
values.

To change these values, proceed as follows:

− Enter the desired parameters

→ Only the selection panel affected by the changes is flashing.

− After entering all set point values, press the desired

program selection key for approx. 3 Seconds:

→ The entering is confirmed by the signal, the new program is
stored.

Repeat the process to set values to additional program
places.

Centrifuging with a program

After closing the centrifuge lid, call the desired program
memory number using the program selection key and
press the start key

.

If the rotor is started with a program the speed or RCF
set point value which exceeds the permissible value of
the inserted rotor, or the RCF/set point which is below
the rotor-specific minimum, the program selection keys
LED of the program chosen beforehand will be
switched off after the rotor identification.

50

Operation

”Quick Run”

For short-time operation, the Multifuge® is equipped
with a "quick run" function

Short-time centrifugation is started by pressing the
"quick run" key

continuously; it stops as soon as the

key is released.
In this mode the centrifuge accelerates with full power

up to the maximum speed. The set speed or RCF is
ignored in this case.

Depending on the rotor, the centrifuge
accelerates to the maximum speed!

Check carefully whether you have to
maintain a specific speed for your
application.

During acceleration the time is counted forward in sec­onds. The display remains until the centrifuge lid is
opened.

.

Removing the rotor

1. Open the centrifuge lid.

2. Remove the rotor cover (on applicable rotors).

3. Unscrew the clamping sleeve counterclockwise

using the socket wrench supplied with the instru­ment until no resistance exists.

4. Grab the rotor with both hands and carefully pull it

perpendicularly off the drive shaft. Make sure not to
tilt it.

Grab rotor with both hands and pull
upwards perpendicularly.

When using an aerosol-tight bio-containment cover,
you may remove the respective rotor from the drive
shaft without opening the cover! You may then open
the rotor e.g. in a safety work bench and decontami­nate it.

51

Operation

Audible alarm

Accompanying all error messages, a warning signal is
given out which can be silenced upon pressing any
key.

You have the option of signaling the end of a run
acoustically. To activate or deactivate this option, press
the program key and simultaneously switch on
the centrifuge. As long as you keep this key pressed,
the selection mode is active.
The speed panel shows "beep", and the time panel
shows "on" or "off".

By actuating the upward-key
signal function can be switched on or off

When the message "rotor" flashes, pressing
the start key once is sufficient to turn off the
warning signal and to accelerate the rotor to
the maximum speed displayed by the in­strument.

in the time panel the

.

Turning off the centrifuge

The centrifuge is turned off by switching the main
switch into the "0"position.

The main power switch should be turned off
after a complete centrifugation run. Without
motor deceleration, it takes much longer
until the rotor comes to a halt.

The centrifuge lid can only be opened automatically if
the centrifuge is turned on!

52

Maintenance and care

Maintenance and care

Maintenance to be performed by the
customer

For the protection of persons, environment and mate­rial you are obliged to clean the centrifuge regularly
and to disinfect it if necessary.

Unsuitable cleaning agents or disin­fection procedures may damage the
centrifuge and its accessories!

If you intend to use cleaning agents
or disinfection procedures not rec­ommended by the manufacturer, you
have to ensure that the foreseen pro­cedure does not cause any damages
to the instrument by consulting the
manufacturer!

Cleaning

Pull mains plug before cleaning the
instrument!

Clean the case, the rotor chamber, the rotor and the
accessories regularly, and in case of need. This is
indicated both for reasons of hygiene and to prevent
corrosion due to contamination sticking to the instru­ment and its accessories.

Clean them with mild agents of pH values ranging from
6 to 8.

For other cleaning agents please consult KENDRO
Services!

Immediately after cleaning, dry the aluminum parts or
put them into a warm-air dryer at a temperature not
exceeding 50°C.

53

Maintenance and care

During cleaning, liquids and espe­cially organic solvents should not
come into contact with the drive
shaft and the ball bearing.

Organic solvents may decompose
the lubricant of the motor bearing.
The drive shaft may seize.

Instruments with refrigeration unit:

If a strong ice sheet is present in the internal
chamber, be sure to remove all condensa­tion after defrosting!

Please clean the venting slots regularly!

Before cleaning the venting slots,
please disconnect the centrifuge
from the mains supply.

Please pull mains plug!

Disinfection

If a centrifuge tube containing infectious material leaks
during a run, you have to disinfect the centrifuge im­mediately.

Infectious material could enter the
centrifuge if spills or tube breakage
occur.

Danger of infection may occur upon
contact! Take appropriate protective
measures for personnel!

Pay attention to the permissible filling
volumes and loading limits for the
tubes!

In case of contamination the operator
has to ensure that no other persons
are at risk!

Contaminated parts will have to be
decontaminated immediately.

If required, further protective meas­ures have to be initiated.

54

Maintenance and care

Rotor and rotor chamber must be treated with a neu­tral, universal disinfectant. Best suited for this purpose
are disinfectant sprays, ensuring that all rotor and ac­cessory surfaces are covered evenly.

• Please use 70% ethanol for disinfection.

Please pay attention to the safety
measures and handling requirements
when applying these substances!

For other disinfectants please consult KENDRO
Services!

• You may disinfect the rotor and the accessories as

described in the following section. Be sure to follow
the pertinent safety procedures for handling infec­tious material.

1. Pull mains plug.

2. Unscrew the rotor chuck.

3. Grab the rotor with both hands and pull it perpen­dicularly off the drive shaft.

4. Remove the centrifuge tubes and adapters, and
disinfect them or dispose of them as necessary.

5. Treat the rotor and the rotor lid according to the
instructions given for the disinfectant (soaking in
liquid or spraying). You must strictly observe the
specified action times!

6. Turn the rotor head down and drain off the disinfec­tant. Afterwards thoroughly rinse rotor and lid with
water.

7. Dispose of the disinfectant according to valid regu­lations.

8. Aluminum rotors have to be treated with anticorro­sive protective oil subsequently.

Disinfection with bleaching lye

These agents contain highly aggres­sive hypochlorites and must not be
used with aluminum rotors!

55

Maintenance and care

Decontamination

For general radioactive decontamination, use a solu­tion of equal parts of 70% ethanol, 10% SDS and wa­ter. Follow this with ethanol rinses, then de-ionized
water rinses, and dry with a soft absorbent cloth. Dis­pose of all washing solutions in appropriate radioactive
waste containers!

Autoclaving

Check whether autoclaving is permitted!

You may autoclave the rotor and the adapters at
121 °C.

Maximum permissible autoclaving cycle: 20 min at
121 °C.

The rotor must be cleaned and rinsed with distilled
water before being autoclaved. Remove the rotor lid,
the centrifuge tubes and the adapters. Place plastic
rotors on an even surface to avoid deformation.

Chemical additives to the steam are not
permitted.

Never exceed the maximum permis­sible values for autoclaving tempera­ture and autoclaving time.

Should the rotor show signs of wear,
you must stop using it!

Corrosion protective oil 7000 9824 is delivered with the
centrifuge.

56

Maintenance and care

The KENDRO service offer

Kendro Laboratory Products recommends annual ser­vicing of the centrifuge and the accessories by author­ized customer service or trained professionals. The
customer service personnel inspect:

• the electrical installations

• the suitability of the location

• the lid lock mechanism and the safety circuit

• the rotor

• the rotor fastening and the drive shaft

Defective material is exchanged.
KENDRO offers inspection and service contracts cov-

ering it. Inspection costs are charged as flat-rate con­tracts.

Necessary repairs are carried out free of charge within
the warranty conditions, and requires payment after
expiration of the warranty period.

Warranty conditions

The warranty period starts on the day of delivery.
Within the warranty period the centrifuge is repaired or
replaced free of charge if there are provable faults in
materials or workmanship.

Conditions for a warranty are:

• the centrifuge is used according to the instructions
of use

• mounting, extensions, settings, alterations or re­pairs are carried out exclusively by personnel au­thorized by KENDRO

• the required maintenance and care procedures are
carried out regularly.

57

Maintenance and care

Notes

58

Troubleshooting

Troubleshooting

Emergency lid release

In case of a power failure the lid cannot be opened
normally using the electrical lid unlocking mechanism.
To permit unloading in this case, the centrifuge is
equipped with an emergency override release. How­ever, you may only use this system in case of emer­gency.

Rotors can spin at high speed! Touch­ing them may cause severe injuries!

Always wait for several minutes until
the rotor has come to a complete stop.
In case of a power outage, the brake
does not function, and deceleration
takes much longer than normal!

Proceed as follows:

1. Make sure that the rotor is at a complete stop
(observe through window in the cover).

During a power outage, it is impossible
to lock the lid once the emergency lid
release has been used. Never stop the
rotor using your hands or using tools!

2. Unplug the main power cord.

3. Facing the centrifuge under the left-hand side, there
is a plastic plug that can be removed using a
screwdriver. After removal, use your fingers or a
pair of pliers to pull the attached cord and activate
the manual door override. The lid will open and you
can remove your samples.

Emergency release

59

Troubleshooting

4. Afterwards, push the cord back into the instrument
and reinsert the plastic plug.

Once the power is restored, you can connect the in­strument to the main supply and turn it on.

Following the self test of the centrifuge, the lid may be
closed and locked with the motor.

60

Error troubleshooting

If problems other than those described in the following tables arise, you must contact
KENDRO service.

Error Symptom Possible causes and corrective measures

Troubleshooting

Displays remain dark The drive stops.

The rotor stops without
deceleration. The lid
cannot be opened.

Displays fail briefly. The drive stops suddenly.

The rotor stops without
deceleration.

Lid cannot be opened.

Pressing the "open lid" key
has no effect.

Mains voltage disconnection

1. Is the mains plug turned on?

2. Check the main connection.

3. If the main connection is ok, contact the nearest KENDRO
service station.

Main connection was briefly interrupted

1. Turn off main switch.

2. Check whether the mains power cord is connected
properly.

3. Restart the centrifuge.

Lid not correctly engaged or lid warped.

1. Check if main connection is working and the instrument is
switched on (display is lit).

2. Press the lid down in the middle of the front section once,
and press the "open lid" key.

3. If this is unsuccessful, you may open the lid using the
emergency lid release (see page 59).

61

Troubleshooting

Error Symptom Possible causes and corrective measures

–

Message "bAl" appears
in display.

Centrifuge is exceptionally
noisy.

Rotor stops with
deceleration.

Imbalance.

1. Stop the centrifuge by pressing the "stop" key, in case of
emergency, unplug mains power cord.

2. Wait until the centrifuge comes to a complete stop.

3. Check whether the rotor is properly loaded.

4. Check whether a broken tube, damage to the rotor or
motor malfunction is responsible for the noise.

If you cannot locate and solve the problem yourself, contact a
KENDRO service station.

Imbalance switch actuated

1. Open the instrument by pressing "open lid" key

..

2. Check whether the rotor is properly loaded.

3. Check whether a broken tube or damage to the rotor was
responsible for imbalance switch actuation.

4. Check that the trunnions of the swinging bucket rotor have
been properly lubricated.

If you cannot locate and solve the problem yourself, contact
the KENDRO service station.

62

Error Symptom Possible causes and corrective measures

Troubleshooting

Message "rotor" appears
in display.

Display "OPEN" appears
although lid is closed.

Message "Lid" appears
flashing in the display.

Message "Lift Lid"
appears in the display

Rotor decelerates with
delayed deceleration.

Will not start.

Drive stops.

Rotor stops without
deceleration to standstill.

Lid does not open
automatically.

Set speed exceeds permissible maximum speed for the
rotor. (The same holds for RCF setting)

A) For about 15 sec. the display shows alternately "rotor" and

the maximum permissible speed or RCF for the installed,
after the rotor identification. Within this period, it is
possible to accept this value by again pressing the "start"
key. The centrifugation will then be continued.

B) Following onset of deceleration you must wait until the

rotor has stopped. By opening and closing the lid you
reset the message "rotor". After entering a permissible
speed you can press start.

Lid not properly closed

Open the lid and repeat locking procedure.

Lid was opened manually during the run.

The instrument stops without deceleration. If you want to
continue the run, you must switch the instrument off and on
again.

The lid has not been unlocked after release.

1. Avoid laying objects onto the centrifuge lid.

2. Lift the lid slightly.

63

Troubleshooting

Error Symptom Possible causes and corrective measures

E-01

|

E-13

E-14

Rotor stops without
deceleration to standstill.

Instrument cannot be
operated.

Rotor stops with
deceleration to standstill.

Instrument cannot be
operated.

E-15
|

E-16

Rotor stops with
deceleration to standstill.
Instrument cannot be
operated.

E-17 Rotor stops with

deceleration to standstill.

Instrument cannot be

Internal program error.

Switch the instrument off and on again. If the error persists,
contact KENDRO service.

Overtemperature in the centrifuge tank.

Switch the instrument off and on again. If the error persists,
contact a KENDRO service station.

Temperature measurement error.

Switch the instrument off and on again. If the problem
persists, contact a KENDRO service station.

Maximum speed for the rotor identification exceeded.

Switch the instrument off and on again. If the problem
persists, a contact KENDRO service station.

operated.

E-18

Instrument stops with
deceleration to standstill
after short starting-up.

Bucket code for this rotor is not defined.

Check, whether the bucket is permitted for the inserted rotor
(rotor program, page 16).

64

Error Symptom Possible causes and corrective measures

Troubleshooting

E-19

E-20

Instrument stops with
deceleration to standstill
after short starting-up.

Instrument does not start or
decelerates to standstill.

No rotor present or rotor identification impossible.

A) Check if a certified rotor is inserted.

B) Please pay attention to the legibility of the inscription of

the swinging bucket rotor cross when it is installed.
(rotor identification must show to the chamber bottom)

C) For the swinging bucket rotor the set bucket type must be

permitted for the operation in the respective rotor.
Please note the hints of chapter "Bucket selection of
swinging bucket rotors" (page 43) and compare the
permitted rotors and rotor buckets in "table 1" on page 16.

D) Following a brief power failure, the rotor could not be

identified. Switch the instrument off and on again using
the mains switch.

Rotor cannot be identified.

A) Check if a certified rotor is inserted.

B) Check if the rotor is completely fastened onto the motor

shaft. For this, release the rotor fastening until you can lift
and depress it easily. Now tighten the rotor fastening once
again.

65

Troubleshooting

Error Symptom Possible causes and corrective measures

E-21

E-22
|

E-23

E-24
|

E-27

E-28

Instrument does not start or
decelerates to standstill.

Rotor stops without
deceleration to standstill.

Instrument cannot be
operated.

Instrument cannot be
operated.

Instrument decelerates
after a short starting-up.

No rotor present or rotor identification impossible

A) Check if a certified rotor is inserted.

B) Following a brief power failure, the rotor could not be

identified. Switch the instrument off and on again using
the main switch.

Error in speed measurement

Switch the instrument off and on again. If the error persists,
contact the KENDRO service station

Wrong status information from lid lock.

Switch the instrument off and on again. If the error persists,
contact the KENDRO service station.

Motor runs in wrong rotational direction.

Switch the instrument off and on again. If the error persists,
contact the KENDRO service station.

66

Error Symptom Possible causes and corrective measures

Troubleshooting

E-29 Motor does not start.

E-30

Rotor stops without
deceleration to standstill or
does not start.

E-31

Rotor stops without
deceleration to standstill or
does not start.

E-32

Rotor stops without
deceleration to standstill or
does not start.

Motor or rotor blocked.

1. Switch instrument off and on again using the main switch.

2. Open the lid.

3. Check whether the rotor can turn freely.

If you cannot clear the malfunction, contact the KENDRO
service station.

Control voltage breaks down.

Switch the instrument off and on again. If the error persists,
contact the KENDRO service station.

Overtemperature in the motor.

1. Turn instrument off and unplug mains power cord.

2. Check and clean ventilation slots if necessary.

3. After about 60 min. you can restart the instrument.
Observe the max. permissible environmental temperature!
If the error persists, contact the KENDRO service station.

.

Overtemperature in the electronics.

1. Turn instrument off and unplug mains power cord.

2. Check and clean ventilation slots if necessary.

3. After about 60 min. you can restart the instrument.
Observe the max. permissible environmental temperature!
If the error persists, contact the KENDRO service station.

67

Troubleshooting

Error Symptom Possible causes and corrective measures

E-33

Rotor stops without
deceleration to standstill or
does not start.

E-34 Rotor stops without

deceleration to standstill or
does not start.

E-35 Rotor stops without

deceleration to standstill or
does not start.

E-36
|

E-38

Rotor stops without
deceleration to standstill.

Instrument cannot be
operated.

E-39

Rotor stops without
deceleration to standstill.

Instrument cannot be
operated.

Overpressure in the refrigeration system

1. Turn instrument off and pull main plug.

1. Check and clean ventilation slots if necessary.

2. After about 60 min. you can restart the instrument.

Observe the max. permissible environmental temperature!
If the error persists, contact the KENDRO service station.

Overvoltage in the intermediate circuit.

Switch the instrument off and on again.

If the error persists, contact the KENDRO service station.

Overcurrent in the intermediate circuit.

Switch the instrument off and on again. If the error persists,
contact the KENDRO service station.

Overcurrent or error in current measurement.

Switch the instrument off and on again.
If the error persists, contact the KENDRO service station.

Speed control measurement exceeds permissible rotor
speed.

Switch the instrument off and on again.
If the error persists, contact the KENDRO service station.

68

Error Symptom Possible causes and corrective measures

Troubleshooting

E-40

Rotor stops without
deceleration to standstill.

Instrument cannot be
operated.

Acceleration of rotor too slow

Open the lid and check the load!

- Significant imbalance caused by missing bucket

- Tubes or adapter do not fit

(rotor is rubbing the chamber or the cover)

- Micro plates carriers on the motor cover

If the error persists, contact the KENDRO service station.

69

Troubleshooting

Contacting Kendro Service

Should you require help from our service station,
please indicate the catalog and serial number of your
instrument. You will find the pertinent information at the
specifications, near the socket for the main plug.

Moreover it is helpful for our service representative to
know your software version
software version as follows:

1. Switch the instrument off.

2. Keep selection key pressed and switch on the
instrument

For about 1 sec all displays read:

. You can determine the

Subsequently, the following readings will be dis­played for 5 seconds each:
(Numbers are examples)

Software version keyboard

Software version

NV-RAM version _4468

__

526 __2
528 __6

__

__

7

The value in the time panel indicates the development
stage.

The last information displayed indicates the current
cycle status.

Cycle counter __235 _CY
The values given are only examples!
During the subsequent program test, the message

_ TEST PRO / 4 ... 1* is displayed.

(* only for instruments with refrigeration system)

70

Technical Data

Features Specification

Technical Data

Ambient conditions

- indoor use

- maximum elevation 2000 m (6562 ft) above sea level

- max. relative humidity 80 % up to 31°C (88°F), linearly decreasing

down to 50 % relative humidity at 40°C (104°F).

Ambient temperature allowed +2 °C to +40 °C (36°F to 104°F)

run time - standard mode : 1min – 9 h 59 min, hold = permanent

- extended mode : 1min – 99 h, hold

maximum speed (n

minimum speed (n

) 15 000 rpm (rotor-dependent, adjustable in steps of 10)

max

) 300 rpm

min

maximum RCF 24 652 (micro liter rotor 48 x 2ml 7500 3348)

maximum kinetic energy Multifuge® 1 S
Multifuge

noise at maximum speed Multifuge® 1 S
Multifuge

®

1 S-R

®

1 S-R

34.3 kNm (25 298 ft.lb)

34.3 kNm (25 298 ft.lb)

< 64 dB (A)
< 54 dB (A)
(sound pressure level of emission according to DIN EN ISO 11 201)

Temperature set range -9 °C to +40 °C

Dimension (H x W x D) Multifuge® 1 S
Multifuge

®

1 S-R

365 mm x 500 mm x 615 mm ( 14.37 x 19.69 x 24.21 inches )
365 mm x 686 mm x 665 mm ( 14.37 x 27.0 x 26.18 inches )

71

Technical Data

Features Specification

Weight without rotor Multifuge® 1 S
Multifuge

Testing standards

- all devices manufactured and examined in
agreement with:

- for 120 V only

- for 230 V only

®

1 S-R

92 kg ( 203 lb )
118 kg ( 259 lb )

IEC 61010-1:1990 + amendment 1:1992 + amendment 2:1995
IEC 61010-2-020:1993 + amendment 1:1996

- Pollution degree 2, - Overvoltage category II

CAN/CSA-C22.2 No. 1010-1.92
CAN/CSA-C22.2 No. 1010-1.B97 amendment 2
UL 3101-1

EN 292
EN 61 010-1, EN 61 010-2-020
EN 61326, EN 55011 B

72

Electrical connections / fuses

Technical Data

Order no. Voltage

Multifuge® 1 S
7500 4311

Multifuge® 1 S
7500 4315

Multifuge® 1 S-R
7500 4331

Multifuge® 1 S-R
7500 4335

Frequency Nominal

current

Power
consumption

230 V 50/60 Hz 9.1 A 1300 W

120 V 60 Hz 12.0 A 1200 W

230 V 50/60 Hz 9.2 A 1520 W

120 V 60 Hz 12.0 A 1440 W

Fuse protection of
instrument

- safety fuse

* thermal excess current
release

12 A
* 14 A

12 A
* 16 A

12 A
* 14 A

12 A
* 16 A

Fuse
protection of
building

16 AT

15 AT

16 AT

15 AT

73

Notes

74

Appendix

Acceleration and deceleration profiles

On the following pages you find acceleration and deceleration profiles for each rotor type respectively.

75

Acceleration profiles

Swinging Bucket Rotor TTH 400 75002000

9 1

5000

4000

3000

speed [rpm]

2000

1000

0

0 30 60 90 120 150 180 210

time [s]

76

p

[

p

]

Deceleration profiles

Swinging Bucket Rotor TTH 400 75002000

5000

4000

3000

m
r

eed

2000

s

1000

0

0 30 60 90 120 150 180 210

1 9

time [s]

77

p

[

p

]

Acceleration profiles

BIOshield

®

600 Rotor 75002005

9 1

6000

5000

m

4000

r

eed

3000

s

2000

1000

0

0 30 60 90 120 150 180 210 240

time [s]

78

p

[

p

]

6000

5000

4000

Deceleration profiles

BIOshield

®

600 Rotor 75002005

m
r

3000

eed
s

2000

1000

0

0 30 60 90 120 150 180 210

1 9

time [s]

79

p

[

p

]

Acceleration profiles

Micro plate carrier MP 3300 75002010

1 9

4000

3000

m
r

2000

eed
s

1000

0

0 30 60 90 120 150 180 210

time [s]

80

p

[

p

]

Deceleration profiles

Micro plate carrier MP 3300 75002010

4000

3000

m

2000

r

eed
s

1000

0

0 30 60 90 120 150 180

9 1

time [s]

81

p

[

p

]

Fixed-Angle Rotor FA12.94 Highconic

Acceleration profiles

®

7500 2006

9 1

11000

10000

9000

8000

7000

6000

m
r

5000

eed

4000

s

3000

2000

1000

0

0 30 60 90 120 150 180 210 240

time [s]

82

p

[

p

]

Fixed-Angle Rotor FA12.94 Highconic

Deceleration profiles

®

7500 2006

m
r

eed
s

9 1

time [s]

83

p

[

p

]

Acceleration profiles

Micro Liter Rotor 48 x 2 ml 75003348

9 1

16000

14000

12000

10000

m

8000

r

eed

6000

s

4000

2000

0

0 30 60 90 120 150 180 210 240

time [s]

84

p

[

p

]

Deceleration profiles

Micro Liter Rotor 48 x 2 ml 75003348

16000

14000

12000

10000

m
r

8000

eed

6000

s

4000

2000

0

0 30 60 90 120 150 180 210 240 270

9 1

time [s]

85

86

[

g]

)

Speed/RCF diagrams

* n

= max. speed

max

Speed/RCF diagram

Swinging Bucket Rotor TTH 400 75002000

10000

1000

x

RCF

100

Round bucket

Rectangular bucket

n

= 4700 rpm

max

RCF

= 4618

max

10

100 1000 10000

speed (rpm

87

[

g]

)

10000

n

= 6000 rpm

max

RCF (r

max nmax

1000

Speed/RCF diagram

BIOshield® 600 Rotor 75002005

) = 6842

x

RCF

100

10

100 1000 10000

88

speed (rpm

[

g]

)

10000

1000

n

= 4400 rpm

max

RCF (r

max nmax

Micro plate carrier MP 3300 75002010

) = 3355

Speed/RCF diagram

x

RCF

100

10

100 1000 10000

speed (rpm

89

[

g]

)

Fixed-Angle Rotor FA12.94 Highconic® 7500 2006

Speed/RCF diagram

100000

10000

1000

n

= 10 350 rpm

max

RCF (r

max nmax

) = 15 090

x

RCF

100

10

1

100 1000 10000 100000

90

speed (rpm

r

= 12.5 cm

max

r

= 6.1 cm

min

[

g]

100000

10000

1000

n

= 15 000 rpm

max

RCF (r

max nmax

) = 24 652

Speed/RCF diagram

Micro liter Rotor 48 x 2 ml 7500 3348

r

= 9.8 cm

max

r

= 5.9 cm

min

x

100

RCF

10

1

100 1000 10000 100000

speed (rpm)

91

92

Index

A

acceleration and deceleration curves · 75
acceleration profiles · 7
Acceleration/decelaration profile · 10
actual values · 44
adapter · 24
aerosol-tight operation · 32
aerosol-tightness

test · 36
aluminum rotor: · 55
approved rotors · 38
audible signal

can be switched on or off · 52

switching on/off · 52
autoclaving · 56
autoclaving cycle

permissible maximum · 56

B

bAL · 48
Basic unit/ function · 9

C

centrifuge exceptionally noisy · 62
Centrifuge transport and installation · 13
Centrifuging with a program · 50
changing settings

during run · 48
cleaning · 53
closing the lid · 37
collet chuck · 38
common glass or plastic centrifuge tubes · 4
conditions of warranty · 57
Conformity · 5
contamination

necessary measures · 54
continuous operation · 46
control panel

readings · 7
corrosion · 4
corrosive substances

protective tubes for corrosive substances · 4
cycle counter · 70

93

D

dangerous chemicals · 3
Deceleration curves · 43
deceleration profiles · 7
decontamination · 54
Description · 7
Description / feature · 9
Diagnostic messages · 11
disinfectant · 55
disinfection · 4

procedure · 55
disinfection with bleaching lye · 55
displays

brief failure · 61

E

EASYsetTM

user interface · 10
EASYset™ control panel · 7
Emergency lid release · 59
Entering parameters · 43
Entering/changing a program · 50
error codes

„Lid“ · 63

„OPEN“ with lid closed · 63

„rotor“ · 63

error codes

„bAl“ · 62
E-01 ... E-40 · 64

F

filling the centrifuge tubes · 40
fixed run time · 45

G

general view · 7, 8, 15, 16, 18, 19, 20, 22, 23
goes forward · 48

H

handling the centrifuge · 4
hazardous substances · 3
hazards

symbols · 5

hints

symbol · 5

hLd · 45, 46

94

I

icons

for denoting dangers and potential damage · 5
imbalance · 40, 41
imbalance · 48
imbalance detection · 8
indoor use · 13
infectious material

precautions in case of tube breakage · 54
Inserting the centrifuge tubes · 41
Installing the rotor · 38

L

lid

blockage · 61
Lid lock with safety check · 8
lid opening · 37
lid release Emergency · 59
lid unlocking mechanism

manual · 59
limited life time · 40
location · 13

M

maintenance · 53
manual lid unlocking · 59
manual stopping · 49
max. tube dimensions · 25
maximum filling volume · 35
Maximum loading · 39
maximum permissible filling volume · 34
maximum permissible RCF · 48
maximum permissible speed · 48
Maximum RCF value · 16, 17, 21
maximum sample density · 3
microtest plates · 31
minimum distance

from wall · 13

O

opening the lid · 37
operation

short-time · 51

organic solvents

not allowed for cleaning · 54

95

P

pathogenic microorganisms

protection against · 4
Plastic sample tubes
Pretemp function · 47
pretemperature-regulations · 47
problems

handling · 61
program display

symbols · 50
Program memory keys · 10
program storing · 50
protective tubes

for corrosive substances · 4

Q

quick run function · 51
Quick run mode · 11

R

radius of centrifugation

for calculation of RCF value · 45
RCF

maximum · 48

RCF / speed display · 43
RCF display

and rotor identification · 44
RCF selection · 10
RCF setpoint · 44
RCF value · 7, 45

relative centrifugal force · 45
RCF-value · 20, 22, 23
RCF-value · 18, 19
readings

of control panel during run · 7
Regulations for prevention of accidents · 5
relative centrifugal force · 45
remain dark · 61
rotor cover · 38
rotor identification · 8
rotor identification · 44

and RCF display · 44
Rotor program for the Multifuge · 16
rotors

approved · 38
run profile · 7
run time

range · 45

selection · 45

S

safety instructions · 3

96