Heraeus Megafuge 1, Megafuge 2 User manual

Page 1
MEGAFUGE 1.0S MEGAFUGE 1.0RS Operating instructions MEGAFUGE 2.0RS User interface "Sepacontrol"
Page 2
User interface "Sepacontrol"
User interface "Sepacontrol"
The user interface "Sepacontrol" from Heraeus is designed for programming and using the operating func­tions. The present description substitutes the corresponding section of your operating instructions.
The figure below shows the user interface of the Megafuge 1.0RS, which is described in detail below. On the last page of the Appendix you will find the same figure enlarged to full-page size.
Key-operated switch
left and right digit selection, respectively
setpoint
display, temperature, over­temperature
setpoint setting setpoint setting
setpoint
change
set
+
enter
copying
into working
memory
open lid
lid
quick
run
start stop
INSTRUMENTS
start/stop key
lid release
Quick Run
release
Quick Run "ready for
start" display
stop release
system
check
programming
error
over-
temperature
key-operated switch
progr.mode
C
BA
Megafuge 1.0RS
program number selection
diagnostic LEDs
lid open
check lid imbalance
system check progr.error
overtemp.
storage in memory
program display
unbalance
display display acceleration/ braking curves
store mode
accel decel
select
call program storage mode
program
execute select select select
RCF,
rotor type,
radius,
special
messages
speed rcf delay ∆t
rotor
radius
"select" keys
run time, start delay
time temp
run time start delay
time temp
operating temperature overtemperature
acceleration deceleration
accel decel
speed RCF
rotor type radius
speed rcf delay ∆t
rotor
radius
assignment LEDs
You can use the key-operated switch to set the operating mode of "Sepacontrol".
Position A: Unlimited programming. You can call or change all programs.
Position B: The program memory is protected against modifications. However, the current centrifugation
program in the main memory can still be modified.
Position C: Program and main memory are protected against modifications. The preset centrifugation
program can be run an unlimited number of times by actuating the control keys. The setpoints can be displayed any time by pressing "set" or the select key ("select").
You can pull out the key in any position. The respective setting is then valid until the next modification.
If you are in a hurry: Setting the centrifugation conditions without storing the parameters.
Prerequisites: The centrifuge is properly installed, the rotor correctly loaded and balanced, the key­operated switch in position A or B.
2
Page 3
User interface "Sepacontrol"
General procedure for all "select" fields:
Press the "set" key. The display selected for the previous run flashes.
Press the "select" key under the desired function field until the red LED next to the parameter to be
changed (e.g. "temp") is lit. Choose the digit to be altered by briefly pressing one of the direction keys to the left and right of the "set" key (
+
count by pressing the
- or – key until the desired number is displayed.
set
) (repeatedly if necessary), and increase or decrease the
By briefly pressing the key you increase or decrease the chosen digit by one step at a time. If you press and hold the chosen key, the display changes continuously upward and downward, respec­tively. Release the key as soon as you have reached the desired value, and fine-tune if necessary by repeatedly pressing the key.
Acknowledge each entry by pressing "enter".
To enter parameters:
1. Press first the "set" and then the "select" key under the four-function field ("speed", "rcf", "rotor", "ra­dius") until the red LED next to "rotor" is lit, and enter the order number of the rotor (only possible when centrifuge is at rest).
Entering the rotor order number is mandatory. The built-in electronics uses this information to rec-
ognize the maximum permissible speed and sees to it that it is not exceeded.
2. Optional: Choose one of the accelerating and braking curves ("accel" and "decel", respectively, possi­ble choices no. 1–9 for both, plus 0 for brakeless deceleration with "decel", see Appendix).
NOTE: If with the braking curve preset to 0, the set speed is lowered during the run, the newly set speed is
triggered with braking curve 1; the preset curve 1 remains unchanged.
3. Optional: Enter the centrifugation radius. You can set the radius in steps of 10 mm and 1 mm, respec­tively, from the nominal radius (maximum value) to the smallest radius compatible with the design.
When preselecting an RCF value (see below), the set RCF value corresponds to the radius en­tered here.
4. Set the speed, or alternatively the relative centrifugal force (RCF, in multiples of the earth gravity g). To do this, choose the respective parameter ("speed" or "rcf") in the four-function field ("speed", "rcf", "ro­tor", "radius"), and enter the desired value.
5. Set the run time. Select "time", and enter the desired run time. The time measured is the one between the start and the end of the braking process.
h.min: Entry and display in hours and minutes, between 0.00 and 9.59 h: Entry and display in hours, between 10 and 99 hld Continuous operation (hld = "hold")
6. Optional: Enter the desired start delay (1 min to 99 hours, display: "delay").
7. Only refrigerated centrifuges (MEGAFUGE series RS): Set the temperature to a value between 0 °C and 40 °C.
8. Optional (only refrigerated centrifuges – MEGAFUGE series RS): Set the overtemperature control to the desired value (minimum 5 K, upper limit set by the built-in microprocessor). The centrifuge switches automatically off when the overtemperature is exceeded (setpoint + ∆t).
NOTE: Altered parameters are accepted by the main memory only if acknowledged with "enter" (or "start"). Otherwise, following a timeout of about 2 min (or about 20 s with the centrifuge running), the instrument switches the display to the actual values without accepting the modifications.
Operation without program
1. Close the centrifuge lid if still open. The yellow LED next to the "lid" key is lit, the "ready for start" dis­play next to the start/stop key (green LED) is activated, and the centrifuge is ready for use.
2. Press the start/stop key. The centrifuge starts according to the factory-set default value with the accel­eration curve no. 9 (acceleration curves see Appendix). The "lid" LED goes out, the lid cannot be opened any more. The very moment the centrifuge starts, the stop function is released (red LED next to the start/stop key is lit).
3. Wait until the instrument stops automatically after completing the set run time ( braking curve, see Ap­pendix). However, you may stop the centrifugation any time by pressing the start/stop key. At this point the green LED is lit anew, e. g. you can continue a manually interrupted run at any time.
3
Page 4
User interface "Sepacontrol"
Turning off the continuous tone after centrifugation: any key for current run, "enter" key in actual value mode for all subsequent runs (in this case brief display "Alert off"; renewed "enter" or mains supply ON/OFF results in "Alert on").
Display
Actual values
If none of the display panels flashes, actual values are displayed; this is true both during a centrifuge run and at rest. The display values changes as soon as the instrument registers new actual values (e. g. speed during a run, temperature at rest, etc.). Following a centrifuge run, the time display reads the re­maining run time (which is different from 0 only in case of premature STOP) or the run time passed (if "hld" has been preselected). This gives the operator an opportunity to check whether the run has been prema­turely interrupted e. g. by a short-term power failure. The actual time display is overwritten with the new actual values only following a renewed start or power OFF/ON.
By pressing the respective "select" key you can switch between the parameters (acceleration or decelera­tion, speed, RCF etc.). The LED for the currently displayed parameter is lit (see figure under "assignment LEDs"). Further explanations see in the following section under "setpoint values".
Setpoint values
In order to display the current setpoint values, always press first the "set" key (display panels flash) and then the key listed below as required.
Program display displays the number of the currently active program after pressing "store
mode" key (01–32)
acceleration/ braking curves
speed, RCF, rotor type, radius
displays the number of the acceleration curve (1–9), upon pressing the "select" key the number of the braking curve (0–9) (see Appendix)
displays the set speed, and upon pressing the "select" key successively the relative centrifugal force (in multiples of the earth gravity g), the rotor type (identical to the rotor or bucket order no.) and the radius setting
run time, start delay displays the set run time and upon pressing the "select" key the start
delay (if set)
temperature, overtemperature displays the temperature setpoint, and upon pressing the "select" key the
overtemperature control (if set; lowest value 5 K)
4
Page 5
User interface "Sepacontrol"
Program settings
Entering settings for multiple runs (permanent storage in the program memory)
Prerequisites: key-operated switch in position A; centrifuge at rest. You have the choice between the two methods described below to permanently store programs in the
program memory:
A Opening the program memory before
1. Press one of the keys
(for increasing the value) or (for decreasing the value) in the "program" field
setting the run parameters
to set the desired number (01 to 32).
2. Open the current memory location by pressing the "store mode" key. The opening is signalled by a flashing display of one of the parameter fields and of the selected program number in the program dis­play.
NOTE: The selected memory location can only be opened if one of the parameter display panels is flash-
ing (press one of the keys
, or "set" if necessary). Upon opening a memory location you cannot
switch to another location.
3. Set the setpoints for the parameters as detailed on page 3.
4. After having completed all settings, press "execute" key. The program number display and the parame­ter display stop flashing, thus showing that the recent settings have been copied from the main memory into the program memory and are available for future runs under the chosen program number.
B Opening the program memory after
setting the run parameters
1. Select the intended program memory location exactly as described under A 1.
2. Set the desired setpoints as detailed on page 3. As soon as you change the first parameter with the "+" or "-" key, the program number display reads "--".
3. Open the program memory selected according to B 1. by pressing "store mode" (cf. A 2.).
4. Store the program by actuating the "execute" key (cf. A 4.).
NOTE: If a memory location opened with "store mode" is not closed by "execute", it is closed automati­cally following a timeout of 2 min. Both program memory and main memory keep their respective former values, i. e. any settings altered in the display are discarded.
Operation with program
You can run preset programs either as stored, or you may change individual settings for the current run. The stored program settings are not modified by changing individual settings (this is possible only via the programming routine, see above).
Prerequisites: The centrifuge is properly installed, the rotor correctly loaded and balanced, the key­operated switch in position A or B.
Program run as stored
1. Program selection: Press one of the direction keys
or until the desired program number is dis-
played.
2. Press the start/stop key within 2 min. The contents of the selected program memory location is loaded into the main memory, and the program is executed immediately. You may stop the centrifugation any time by pressing the start/stop key once again.
Program run with modifications
1. Program selection: Press one of the direction keys
or until the desired program number is dis-
played.
2. Press the "execute" or "enter" key, the program settings are loaded into the main memory.
3. Make the desired changes as detailed on page 3. The program display reads "--" as a reminder that the current settings do no longer correspond to those of the originally selected program. However, the original, stored program is unchanged and still available.
4. Press the start/stop key. The program run is started at once. You may stop the centrifugation any time by pressing the start/stop key once again.
5
Page 6
User interface "Sepacontrol"
"Quick-Run" operation
Independent of set values you can, with the lid closed, start a centrifuge run using the "Quick-Run" key.
ATTENTION – WARNING!
As long as the "Quick-Run" key is pressed, the rotor is accelerated with maximum force to its maximum permissible speed, i. e. the set speed is ignored.
The run is continued until the key is released, upon release the rotor is braked with maximum force. By pressing the key once again, you can switch back to acceleration.
6
Page 7
Appendix
Diagnostic LEDs
LED "lid"
check lid
Appendix
Centrifuge lid not closed.
LED "imbalance"
Rotor not evenly loaded.
rotor imbalance
LED "system check"
Electronics malfunction.
system check
LED "program error"
Operating error.
program error
LED "overtemp."
The set temperature limit is exceeded.
overtemperature
Malfunctions – measures for self-help
Malfunction Cause Remedy
Symptom Behavior
Set position flashes upon pressing the "set key
Setting with "+/-" keys impossible, lid cannot be opened, start and
Position of key-operated switch prevents programming.
stop OK.
Displays re­main dark
Drive stops sud­denly, rotor stops
Power failure. Check power supply, if OK call
without braking, lid cannot be opened.
Turn key-operated switch from position C to A or B, if no re­sponse, call Service.
Service.
Displays fail briefly
Unusually loud running noise, possibly with display "imbal­ance"
"OPEN" mes­sage displayed
"check lid" LED is lit and inter­val tone sounds
Drive stops sud­denly, rotor stops without braking.
Drive fails, brakes or keeps running noisily.
Centrifuge cannot be started; lid ap­parently closed.
Drive is turned off and coasts brake­less to standstill.
Short-term power failure. Start anew.
1. Sudden imbalance due to broken vessel and mass shift.
2. Rotor or drive damage.
3. Malfunction in the control.
If the centrifuge does not stop by itself, press "quick run" key Look for apparent cause at standstill and relieve if possible. Other­wise call Service.
4. Damaged fans or compres­sor.
1. Lid lock not properly en­gaged.
2. Lid lock defective.
1. Lid lock was opened manu­ally during the run, action not permitted!
2. Lid lock safety circuit has tripped.
Press lid forcefully down, do not bang! If LED does not go out, call Ser­vice.
1. Press lid shut immediately, switch mains supply OFF/ON, press "start" to resume cen­trifugation, otherwise "stop".
2. Safety circuit malfunction, call Service.
7
Page 8
Appendix
Malfunction Cause Remedy
Symptom Behavior
"imbalance"­LED is lit, inter­val tone
"overtemp"­LED LED is lit, interval tone
Centrifuge brakes maximally with braking profile 9.
After starting:
Drive switched off, refrigerating unit running.
1. Rotor unevenly loaded.
2. Bucket does not swing out properly or not at all.
3. Sudden vessel breakage with center of gravity shift by leaking liquid.
4. Defective drive mechanism (damaged motor shaft).
5. Mechanical or other changes on rotor or buckets.
6. Centrifuge tilted.
t-alarm is activated because the rotor chamber has not been precooled to temperature set­point.
1. Distribute buckets, inserts and fillings evenly.
2. Clean and grease (#70006692) rotor pin and opposite surface on the bucket.
3. Remove splinters, broken parts and sample residues, clean buckets and fill anew, restart with reduced speed if necessary.
4. Do not start drive any more! Call Service.
5. Discontinue use of rotor and/or buckets! Inform Ser­vice.
6. Align centrifuge (drive), call Service if necessary.
Open lid, turn off t ("--"), close lid and start; wait until cooled to desired setpoint temperature range, then activate ∆t.
During operation: Centrifuge stops without braking, refrigerating unit running.
"E-17" display, "system
Upon pressing "lid"
key at standstill check"-LED is lit, interval tone
"system check"-LED is lit, interval tone "E-xx" display flashes in the speed panel, other possible xx numbers 00-
During operation:
Drive coasts
brakeless to
standstill.
At standstill:
Drive switched off
after start.
24
Overtemperature alarm, dis­played temperature exceeds permissible threshold value: temperature of the environment (35 °C max.) or distance to wall (15 cm).
1. t dependent: cooling capac­ity of refrigerating unit insuf­ficient
2. No ∆t programmed: defective refrigerating unit
1. Lid lock mechanism jammed, does not open
2. Lid lock defective
Tripping of a thermal or electri­cal protection function, e. g. E-
08.
Malfunctions in data transmis­sion or measuring.
Wait until centrifuge has cooled, possibly remove and cool sam­ples after standstill, precool cen­trifuge and run again if neces­sary, do not operate near heat generators.
Repeat run with reduced speed, if deviations are considerable call Service.
Call Service.
Mains supply OFF, check lid lock mechanism and correct if neces­sary.
Call Service.
Switch off centrifuge and resume operation after a minimum of 15 min, if error occurs repeatedly call Service.
Mains supply OFF/ON, if error persistent call Service.
8
Page 9
Appendix
Technical data of "Sepacontrol"
Feature Possible settings
Program memory for 32 complete sets of parameters Program backup unauthorized intervention in stored programs lockable via a three-step
key-operated switch Data storage almost unlimited with NV-RAMs Diagnostic messages
via LEDs for
lid not closed during operation
unbalanced loading
general malfunction of the instrument
data transmission error
overtemperature
Digital parameter display for program number, acceleration and braking profiles, speed, RCF,
rotor, radius, time, start delay, temperature and overtemperature Acceleration curves 1–9 profiles selectable Braking curves 1–9 profiles and 0 = coasting to standstill Speed selection continuously between 100 min
1000, 100 and 10 min
-1
-1
and maximum speed in steps of
RCF selection continuously between 1 x g and maximum RCF value in steps of
1000, 100, 10 and 1 x g Rotor selection integral numbers, organized according to order numbers in ascending
order Radius selection dependent on rotor in 0.1-cm steps Time selection in 3 modes:
hld continuous operation
h.min 1 min – 9 h 59 min
h 10 h – 99 h
Start delay in 3 modes:
-- immediate start (no delay)
h.min 1 min – 9 h 59 min
h 10 h – 99 h
Time display with "Quick Run":
min. sec mode 1 s – 9 min 59 s
t mode 10 h – 99 h Temperature selection 0 °C – 40 °C in steps of 10 K and 1 K
Overtemperature control ∆t:
-- no overtemperature control
5 K ≤ ∆t ≤ (40 °C –T
5 K ≤ ∆t ≤ 10 K
) if 0 °C ≤ T
setpoint
if 31 °C ≤ T
setpoint
setpoint
30 °C
40 °C
adjustable in 1 K steps
9
Page 10
Appendix
Acceleration and braking curves
Acceleration curves
speed [rpm]
4000
3000
2000
1000
23456789
1
0 60 120 180 240 300
Braking curves
4000
3000
2000
speed [rpm]
1000
8
9
7
6
time [s]
5
4
3
2
1
10
0
0 60 120 180 240 300 360 420
time [s]
Page 11
Appendix
Quick Run
release
Quick Run
"ready for
stop
start"
release
display
lid release
lid
open lid
run
start
quick
stop
INSTRUMEN T S
start/stop key
operating temperature
setpoint setting
overtemperature
set
setpoint
display,
setpoint
change
left and right digit selection, respectively
temperature,
over-
temperature
run time,
start
delay
temp
time
enter
+
select
t
select
delay
copying
into working
memory
t
temp
setpoint setting
run time
start delay
delay
time
diagnostic
display
RCF,
display
program display
LEDs
rotor type,
acceleration/
lid open
radius
RCF
rotor
speed
accel
radius
assignment LEDs
rcf
decel
rotor
radius
select
radius,
special
messages
rcf
speed
accel
decel
braking
curves
select
"select" keys
rotor type
call
program
storage
mode
speed
store
mode
program
execute
unbalance
storage in memory
check lid
imbalance
system check
progr.error
overtemp.
progr.mode
A
acceleration
B
C
Megafuge 1.0RS
deceleration
program number selection
key-operated switch
error
check
system
programming
over-
temperature
11
Page 12
Appendix
Contents
User interface "Sepacontrol" .........................................................................................2
Key-operated switch.....................................................................................................................................2
If you are in a hurry: Setting the centrifugation conditions without storing the parameters.........................2
Entering parameters.................................................................................................................................3
Operation without program ..........................................................................................................................3
Display..........................................................................................................................................................4
Actual values ............................................................................................................................................4
Setpoint values.........................................................................................................................................4
Program settings ..........................................................................................................................................5
Entering settings for multiple runs (permanent storage in the program memory) ...................................5
A Opening the program memory before
B Opening the program memory after setting the run parameters ..........................................................5
Operation with program ...............................................................................................................................5
Program run as stored..............................................................................................................................5
Program run with modifications................................................................................................................5
"Quick-Run" operation..................................................................................................................................6
Appendix ..........................................................................................................................7
Diagnostic LEDs...........................................................................................................................................7
Malfunctions – measures for self-help .........................................................................................................7
Technical data of "Sepacontrol"...................................................................................................................9
Acceleration and braking curves............................................................................................................... 10
Acceleration curves ............................................................................................................................... 10
Braking curves....................................................................................................................................... 10
setting the run parameters .......................................................5
12
Page 13
Kendro Laboratory Products GmbH Postbox 15 63 D-63405 Hanau / Germany Telephone: (+49) 61 81/ 35-300
Telefax: (+49) 61 81/ 3 57 49, Sales (+49) 55 22/ 31 62 76, Service Telephone: (+49) 55 22/ 31 62 22, Service
In the interest of continuous product development, we reserve the right to make changes without express notice.
20056899 Mega S_GB 10/98 Printed in Germany
Loading...