These operating instructions are protected by copyright. Rights resulting thereof, particularly reprint, photomechanical or digital postprocessing or reproduction, even in part, are only allowed with the written consent of Kendro Laboratory
Products GmbH.
This regulation does not apply to reproductions for in-plant use.
Trademarks
®
HERAsafe
and Heraeus are registered trademarks of Kendro Laboratory Products GmbH. All other trademarks mentioned in the operating instructions are
the exclusive property of the respective manufacturers.
The following are the addresses of the international Kendro
Sales Organisations.
Postal address Germany
Kendro Laboratory Products GmbH
Robert-Bosch-Straße 1
D - 63505 Langenselbold
Anfragen aus Deutschland
Telefon
Vertrieb0800 1 536376
Service0800 1 112110
Fax
Vertrieb/Service0800 1 112114
E-Mailinfo@kendro.de
Enquiries from Europe, Middle East
and Africa
Tel.+ 49 (0) 6184 / 90-6940
Fax+ 49 (0) 6184 / 90-7474
E-Mailinfo@kendro.com
Postal address USA
Kendro Laboratory Products Inc.
275 Aiken Road
Asheville, NC 28804
USA
Enquiries from North America
Phone+1 800-879 7767
Fax+1 828-658 0363
E-Mailinfo@kendro.com
Enquiries from Latin America
Phone+1 828-658 2711
Fax+1 828-645 9466
E-Mailinfo@kendro.com
Enquiries from Asia Pacific
Phone+852-2711 3910
Fax+852-2711 3858
E-Mailinfo@kendro.com
1.1Safety instructions
These safety instructions describe the safety features of the HERAsafe series
and apply to the models KS 9/12/15/18.
The safety cabinet has been manufactured in keeping with the latest technological developments and has been tested before delivery for its correct function. It may, however, present potential hazards if it is not used according to the
intended purpose or outside of operating parameters. Therefore, the following
procedures must always be observed:
•The safety cabinet must be operated only by trained and authorized personnel.
•For any operation of this unit, the operator must prepare clear and concise
written instructions in the language of the operating and cleaning personnel
based on these operating instructions, applicable safety data sheets, plant
hygiene guidelines, and technical regulations, in particular:
•which decontamination measures are to be applied for the cabinet and
accessories,
•which protective measures apply while specific agents are used,
•which measures are to be taken in the case of an accident.
•Repairs to the device must be carried out only by trained and authorized expert
personnel.
• The contents of the operating instructions are subject to change without
further notice.
•Concerning translations into foreign languages, the German version of these
operating instructions is binding lease.
•Keep these operating instructions close to the unit so that safety instructions
and important information are always accessible.
•Should you encounter problems that are not detailed adequately in these operating instructions, please contact Kendro Laboratory Productsimmediately for your own safety.
6
1.General notes
1.2Warranty
Kendro Laboratory Products warrant the operational safety and functions of the
safety cabinet only under the condition that:
•the device is operated and serviced exclusively in accordance with its intended
purpose and as described in these operating instructions,
•the device is not modified,
•only original spare parts and accessories that have been approved by Kendro
Laboratory Products are used,
•inspections and maintenance are performed at the specified intervals,
•an installation test is performed prior to the initial operation of the device and
that a repeat test is performed on the occasion of all inspections and repairs.
The warranty is valid from the date of delivery of the device to the operator.
The safety cabinet is a laboratory device for installation and operation in microbiological and biotechnical laboratories of safety levels 1, 2, and 3. It has been
designed as a Class II microbiological safety cabinet, in accordance with EN
12469 / 2000.
Depending on the hazard level of the agents involved, the operator must prepare in writing appropriate decontamination procedures for the device and the
accessories used in the sample chamber.
KSP version:
The safety cabinet also meets the requirements from DIN 12980 of 2/2004 and
can be used for the production of cytostatic agents.
For cytostatic agent applications, use the lowered one-piece working plate only.
Prior to the initial operation of the cabinet, the operator must perform an installation test. The test result must be documented by a test report. The cabinet must
only be released for operation if it is in compliance with the operating parameters specified by Kendro Laboratory Products.
After any changes to the installation conditions or after any modification to the
technical system, a repeat test must be performed and the test result must be
documented by a test report that shows that all operating parameters are in compliance with those specified by Kendro Laboratory Products.
1.4.2Incorrect use:
The safety cabinet must not be used in laboratories that do not comply with the
requirements of safety levels 1, 2, and 3.
The unit must not be operated as a Class II safety cabinet, if:
•no repeat test is performed after changes to the installation conditions or after
modifications to the technical system,
•the alarm system of the device has issued a failure message and the cause for the failure has not been repaired.
The alarm system must not be tampered with or disabled. If alarm system components heve been removed or disabled for service or repairs, the unit must only
be released for operation if all alarm system components are functioning again
properly.
The filters installed in the device are not capable of separating gaseous substances. Therefore, do not work with or store substances in the device:
•who in quantity or concentration are toxic,
•if a reaction with other substances may result in hazardous toxic concentrations
or formation of toxic gases,
•that may form combustible or explosive mixtures in combination with air.
The operational safety and correct function of the unit depend on the location
where it is to be operated. The safety cabinet must be operated only at locations that meet the ambient conditions listed below.
Location requirements:
•The electrical system of the device has been designed for an operating height
of up to 2000 m above sea level.
•The mains power supply outlets should be out of casual reach to prevent accidental shut-off. Ideally, the outlets should be installed above the safety
cabinet.
•The flooring of the location must be adequately strong and not flammable.
•The room in which the device is installed must be of adequately height. For
units not connected to an exhaust system, the distance between the exhaust
air opening and the room ceiling must be at least 200 mm (8 in).
•The location must be equipped with an appropriate ventilation system
(see Section 3.2.).
•The temperature within the room must be between 15° C and 40° C
(49° F and 104° F).
•The relative humidity in the vicinity of the device must not exceed 90 %.
NOTE - Ambient conditions!
If ambient conditions vary from those described above, please contact Kendro Laboratory Products for assistance in installing the
device.
NOTE - Temporary storage!
If the device is stored only temporarily (up to
four weeks), the ambient temperature may be
between -20° C and +60° C (-4° F and +140° F)
at a relative air humidity of up to 90 %. For longer storage periods, the location requirements
apply.
3.2Room ventilation
The room ventilation should preferably be a ventilation system that complies with
the national requirements for the application.
•The inlet air and exhaust air openings of the room ventilation must be located
so that drafts are prevented from impairing the function of the safety cabinet air system.
12
3.Installation
3.3Correct location
Choose a draft-free location where the safety cabinet does not
interfere with the plant traffic.
Fig. 1: This figure shows preferred locations for safety cabinets and unsuiable locations, not in accordance with the safety
requirements.
Unsuitable locations: The locations [1], [2], and [3] are not
suitable because they are exposed to drafts from windows and
doors.
Location [5] is undesirable because it is in range of plant traffic
and within the exhaust air range of a ventilation system [4].
Preferred locations [6], [7], and [8] are correct because they
are in a draft-free section of the room and not exposed to plant
traffic.
When several devices are to be installed in series, please observe the following:
•Make sure that vibrations cannot be transferred between
adjacent units.
•Exterior surfaces of the cabinets must always be accessible
for cleaning and disinfection.
3.5Transport
Version KS:
Fig. 2: To prevent tilting, always transport the device using a
suitable carrier, even for a transport within a building, and separate it from the rack.
NOTE – Lift points
For transport, lift the unit only at the lift points
shown in Figure 2.
Do not allow the weight of the cabinet to rest
on the floorpan!
Version KSP:
If required, the device frame must be removed from the rack
to be reinstalled onto the rack for the initial start-up after the
transport (see Section 5.2).
•Fig. 3a: Plenum assembly [5] with plenum for downflow blower [24] and
plenum for exhaust air blower [2]. The downflow filter and the exhaust air filter
are installed directly to the pertaining blower. The exhaust air is released into
the environment around the device through the opening [1].
•At the side of the plenum, the optional media supply lines [25] are routed into
the sample chamber [20].
•Electrical box [4] with power supply cable [3]. An RS 232 connection to a PC
and two fuse holders are installed at the front of the electrical box.
•Front cover [6] with integral, electrically movable front window [7], operated by:
•the pilot switch [26],
•the remote control [28].
The gas struts [22] secure the front cover in the open state.
Optionally, two safety latches [27] can be installed to the front cover so that
the door can be protected against unauthorized opening.
•The light dome [23] with two tubes is part of the front cover assy.
•Side slates [9] with two sealed access openings [19]. These can be equipped with media valves [13].
•Internal outlets [10] for power supply of accessories and adapter [11] for mobile
UV device.
•Optional UV lamp unit [8] consisting of two UV lamps per side.
•Optional stands [12], adjustable in height (KFS 2) and with fixed height
(KFS 1).
•Workplate segments [15] with optional arm rests [16]. A one-piece workplate
and special workplates are available options.
•Lockable drain valve [17] for installation into floorpan.
•Display [21] with remote control sensor and alarm system LEDs.
•Test hoses for the supply unit [18] at the left side of the sample chamber and
for the exhaust unit [14] at the right side of the sample chamber.
14
NOTE – Test hoses!
Do not remove the two test hoses for checking
downflow and exhaust air.
• Fig. 3b: Rack [5] with integral prefilter housing [6].
• Filter inserts [2] for the prefilter housing. The number of supplied filter inserts depends on the width of the device. To protect from liquid residues,
the filter inserts are oriented slightly sloping from the device backpanel to
form a drain duct to the floorpan [3].
The filter plates [4] have tongues and grooves and are inserted in an overlapping pattern from right to left.
•Membrane sleeve or opening for filling with aerosole for testing the filter of
the downflow unit [1] at the left side of the sample chamber and of the exhaust air unit [7] at the right side of the sample chamber.
16
Overall view / Version KSP
Fig. 3b
4.Unit description
4.2Safety system
The safety system comprises a combination of protective and alarm systems
that ensure maximum personal and material protection.
Safety systems:
•Vacuum-sealed air system
A vacuum-sealed air system in combination with HEPA filters for downflow
and exhaust air forms the basis of the safety system for personal and material protection.
•Personal protection
Air aspired from the exterior along the entire working opening at a constant
high velocity prevents that:
• agents may leak through the working opening of the chamber.
As the exterior air pressure around the unit exceeds the pressure of the internal air system (vacuum sealing), it is ensured that:
• agents cannot be released to the exterior in the case of a leak in the
cabinet housing.
•Material protection
A steady airflow within the air system ensures that:
•a constant downflow allows the HEPA filters to remove contaminants
so that the samples are always surrounded by ultrapure air,
•harmful particles are not carried over through the sample chamber
(protection from cross-contamination).
•HEPA filters
The downflow (i.e. the air circulating within the device) and the exhaust air
(air that is released to the exterior) are cleaned by HEPA filters (HEPA = High
Efficiency Particulate Air Filter).
For version KSP, a prefiltering system is used to increase the filter efficiency and to protect the exhaust air and downflow systems.
•Safety lockout
To protect from UV radiation, the optional UV disinfection routine can be run
only if the front opening is closed. During UV disinfection, the front opening
safety lockout is activated and prevents harmful UV radiation from being
emitted from the sample chamber.
Airflow monitoring determines the velocity of the airflow in the sample chamber
as well as the inflow velocity of the air aspired from the exterior through the
working opening. As soon as airflow velocities move above or below a specified safety value, a signal is transmitted to the alarm system.
•Visual and audible alarm system
The warning system constantly monitors the safety-relevant device functions:
•Inflow velocity of the air aspired from the exterior,
•downflow velocity,
•working position of the front window.
If the warning system detects changes to one of these device functions, it
issues:
•Position monitoring
The position sensors monitor the position of the front cover as well as the movement of the front window; it will indicate when the front window is in the working position.
•Kendro Performance Factor
The Kendro Performance Factor (PER) is a value that indicates the safety state of the safety cabinet. This value is
calculated from data determined by the safety system and
from values captured empirically by service personnel during
safety checks. This data is entered into a parameter list of
the control software and interconnected. The result can be
indicated by the display.
4.3Filter system
Version KS:
Fig. 4: The filter system consists of two HEPA filters [2] and
[5] for the circulating air and for the exhaust air and of a coarse
filter for the aspired air.
HEPA filters: Room air [10] is drawn into the sample chamber through the working opening. In the air duct, room air and
the downflow within the chamber [7] are then blended to make
up the blend air [9]. The blend air is then:
•filtered proportionally by the downflow filter [5] and supplied as ultrapure air [6] evenly into the sample chamber of
the device,
•filtered by the exhaust air filter [2] and released as ultrapure air [1] to the exterior of the device.
Inlet air protection: The air duct between the sample chamber
and the device plenum has an inlet air protection [8] below the
working surface to prevent coarse particles from entering the
plenum where they may impair blower [3] und [4] and filter
functions.
Filter system with prefilters,
Fig. 4a
downflow filter and exhaust air
filter / Version KS
18
4.Unit description
Version KSP:
Fig. 4b: The filter system consists of a set of HEPA prefilters
[10] (the number of filters depends on the sample chamber
width) and two HEPA filters [2] and [5] for downflow air and
exhaust air.
HEPA prefilters:
The compact size of the filter inserts allows filter replacement
with minimal contamination hazard. The use of the prefilters
protects the downflow and exhaust air filters considerably.
The filter inserts used are HEPA filters of Class H14 (according to DIN EN 1822). Alternatively, activated carbon filters can
be inserted into the housing to protect from smells.
Room air [11] is drawn into the sample chamber through the
working opening. In the air duct above the prefilter, room air
and the downflow within the chamber [7] are then blended to
make up the blend air [8]. The blend air is then:
•aspirated into the prefilter [10], filtered and fed into the air
duct [9].
Fig. 5: The safety cabinet is equipped with two separate control
elements that operate independently of each other:
•remote control [3],
•pilot switch [1].
The status indicators of the display [2] indicate control operations initiated with the control elements.
Remote control: All device functions can be activated and
deactivated easily using the remote control.
Pilot switch: Use to control all basic functions required for the
operation. This switch allows you to operate the safety cabinet if the remote control is not operational or available.
Fig. 6: The display [1] can show text or numeric values and
has 12 LEDs [2] and [4] to indicate the current operational state
of the unit.
The display module also houses the sensor system for the remote control. The pulses transmitted are best received by the
sensor if the distance A between the remote control [5] and the
sensor [3] does not exceed 1m and if the radio signal beam
does not deviate more than 15° horizontally.
The transmission range of the remote control also depends on
the battery state of charge.
Fig. 5
Controls and display
20
Display with remote control
Fig. 6
sensor
4.Unit description
4.5Sample chamber access
Front cover:
The sample chamber of the device is accessible via two modes:
Fig. 7a: Manual opening of the front cover [2] allows access
to the complete sample chamber width with an opening height
C. It is generally needed for decontamination and introduction
of larger accessories.
NOTE – Front cover lockout!
The front cover is equipped with a safety lockout and can only be opened in standby mode,
i.e. when the front window is completely
closed.
As an option, the front cover of both versions can be equipped with a safety feature: Two safety latches at the left and right
frame struts secure the front cover from unauthorized opening.
With this equipment, the function of the pilot switch is overridden
by a safety bezel so that only the remote control can be used
to access the sample chamber.
If the remote control is faulty, the safety bezel can be unscrewed
and the device can be operated using the pilot switch (see Section 6.4).
Front window:
Fig. 7b: The electrically operated front window [1] is made of
multi-layer safety glass and integral to the front cover frame.
It can be raised to a maximum opening height B. To access
the sample chamber during the work process, the front window must stay in the work position with opening height A.
CAUTION – Front window movement!
Do not attempt to move the front window
manually as otherwise the motor drive may be
damaged.
Access through front cover
Fig. 7a
Lowering the front window when the device is deenergized:
The safety feature (optional) is backed up by a battery. Should
a power failure occur, the pilot switch (see Section 6.4) can be
used to lower the front window completely.
Access through front window
Fig. 7b
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