These operating instructions are protected by copyright. Rights resulting thereof, particularly reprint, photomechanical or digital postprocessing or reproduction, even in part, are only allowed with the written consent of Kendro Laboratory
Products GmbH.
This regulation does not apply to reproductions for in-plant use.
Trademarks
®
HERAsafe
and Heraeus are registered trademarks of Kendro Laboratory Products GmbH. All other trademarks mentioned in the operating instructions are
the exclusive property of the respective manufacturers.
The following are the addresses of the international Kendro
Sales Organisations.
Postal address Germany
Kendro Laboratory Products GmbH
Robert-Bosch-Straße 1
D - 63505 Langenselbold
Anfragen aus Deutschland
Telefon
Vertrieb0800 1 536376
Service0800 1 112110
Fax
Vertrieb/Service0800 1 112114
E-Mailinfo@kendro.de
Enquiries from Europe, Middle East
and Africa
Tel.+ 49 (0) 6184 / 90-6940
Fax+ 49 (0) 6184 / 90-7474
E-Mailinfo@kendro.com
Postal address USA
Kendro Laboratory Products Inc.
275 Aiken Road
Asheville, NC 28804
USA
Enquiries from North America
Phone+1 800-879 7767
Fax+1 828-658 0363
E-Mailinfo@kendro.com
Enquiries from Latin America
Phone+1 828-658 2711
Fax+1 828-645 9466
E-Mailinfo@kendro.com
Enquiries from Asia Pacific
Phone+852-2711 3910
Fax+852-2711 3858
E-Mailinfo@kendro.com
1.1Safety instructions
These safety instructions describe the safety features of the HERAsafe series
and apply to the models KS 9/12/15/18.
The safety cabinet has been manufactured in keeping with the latest technological developments and has been tested before delivery for its correct function. It may, however, present potential hazards if it is not used according to the
intended purpose or outside of operating parameters. Therefore, the following
procedures must always be observed:
•The safety cabinet must be operated only by trained and authorized personnel.
•For any operation of this unit, the operator must prepare clear and concise
written instructions in the language of the operating and cleaning personnel
based on these operating instructions, applicable safety data sheets, plant
hygiene guidelines, and technical regulations, in particular:
•which decontamination measures are to be applied for the cabinet and
accessories,
•which protective measures apply while specific agents are used,
•which measures are to be taken in the case of an accident.
•Repairs to the device must be carried out only by trained and authorized expert
personnel.
• The contents of the operating instructions are subject to change without
further notice.
•Concerning translations into foreign languages, the German version of these
operating instructions is binding lease.
•Keep these operating instructions close to the unit so that safety instructions
and important information are always accessible.
•Should you encounter problems that are not detailed adequately in these operating instructions, please contact Kendro Laboratory Productsimmediately for your own safety.
6
Page 7
1.General notes
1.2Warranty
Kendro Laboratory Products warrant the operational safety and functions of the
safety cabinet only under the condition that:
•the device is operated and serviced exclusively in accordance with its intended
purpose and as described in these operating instructions,
•the device is not modified,
•only original spare parts and accessories that have been approved by Kendro
Laboratory Products are used,
•inspections and maintenance are performed at the specified intervals,
•an installation test is performed prior to the initial operation of the device and
that a repeat test is performed on the occasion of all inspections and repairs.
The warranty is valid from the date of delivery of the device to the operator.
The safety cabinet is a laboratory device for installation and operation in microbiological and biotechnical laboratories of safety levels 1, 2, and 3. It has been
designed as a Class II microbiological safety cabinet, in accordance with EN
12469 / 2000.
Depending on the hazard level of the agents involved, the operator must prepare in writing appropriate decontamination procedures for the device and the
accessories used in the sample chamber.
KSP version:
The safety cabinet also meets the requirements from DIN 12980 of 2/2004 and
can be used for the production of cytostatic agents.
For cytostatic agent applications, use the lowered one-piece working plate only.
Prior to the initial operation of the cabinet, the operator must perform an installation test. The test result must be documented by a test report. The cabinet must
only be released for operation if it is in compliance with the operating parameters specified by Kendro Laboratory Products.
After any changes to the installation conditions or after any modification to the
technical system, a repeat test must be performed and the test result must be
documented by a test report that shows that all operating parameters are in compliance with those specified by Kendro Laboratory Products.
1.4.2Incorrect use:
The safety cabinet must not be used in laboratories that do not comply with the
requirements of safety levels 1, 2, and 3.
The unit must not be operated as a Class II safety cabinet, if:
•no repeat test is performed after changes to the installation conditions or after
modifications to the technical system,
•the alarm system of the device has issued a failure message and the cause for the failure has not been repaired.
The alarm system must not be tampered with or disabled. If alarm system components heve been removed or disabled for service or repairs, the unit must only
be released for operation if all alarm system components are functioning again
properly.
The filters installed in the device are not capable of separating gaseous substances. Therefore, do not work with or store substances in the device:
•who in quantity or concentration are toxic,
•if a reaction with other substances may result in hazardous toxic concentrations
or formation of toxic gases,
•that may form combustible or explosive mixtures in combination with air.
The operational safety and correct function of the unit depend on the location
where it is to be operated. The safety cabinet must be operated only at locations that meet the ambient conditions listed below.
Location requirements:
•The electrical system of the device has been designed for an operating height
of up to 2000 m above sea level.
•The mains power supply outlets should be out of casual reach to prevent accidental shut-off. Ideally, the outlets should be installed above the safety
cabinet.
•The flooring of the location must be adequately strong and not flammable.
•The room in which the device is installed must be of adequately height. For
units not connected to an exhaust system, the distance between the exhaust
air opening and the room ceiling must be at least 200 mm (8 in).
•The location must be equipped with an appropriate ventilation system
(see Section 3.2.).
•The temperature within the room must be between 15° C and 40° C
(49° F and 104° F).
•The relative humidity in the vicinity of the device must not exceed 90 %.
NOTE - Ambient conditions!
If ambient conditions vary from those described above, please contact Kendro Laboratory Products for assistance in installing the
device.
NOTE - Temporary storage!
If the device is stored only temporarily (up to
four weeks), the ambient temperature may be
between -20° C and +60° C (-4° F and +140° F)
at a relative air humidity of up to 90 %. For longer storage periods, the location requirements
apply.
3.2Room ventilation
The room ventilation should preferably be a ventilation system that complies with
the national requirements for the application.
•The inlet air and exhaust air openings of the room ventilation must be located
so that drafts are prevented from impairing the function of the safety cabinet air system.
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Page 13
3.Installation
3.3Correct location
Choose a draft-free location where the safety cabinet does not
interfere with the plant traffic.
Fig. 1: This figure shows preferred locations for safety cabinets and unsuiable locations, not in accordance with the safety
requirements.
Unsuitable locations: The locations [1], [2], and [3] are not
suitable because they are exposed to drafts from windows and
doors.
Location [5] is undesirable because it is in range of plant traffic
and within the exhaust air range of a ventilation system [4].
Preferred locations [6], [7], and [8] are correct because they
are in a draft-free section of the room and not exposed to plant
traffic.
When several devices are to be installed in series, please observe the following:
•Make sure that vibrations cannot be transferred between
adjacent units.
•Exterior surfaces of the cabinets must always be accessible
for cleaning and disinfection.
3.5Transport
Version KS:
Fig. 2: To prevent tilting, always transport the device using a
suitable carrier, even for a transport within a building, and separate it from the rack.
NOTE – Lift points
For transport, lift the unit only at the lift points
shown in Figure 2.
Do not allow the weight of the cabinet to rest
on the floorpan!
Version KSP:
If required, the device frame must be removed from the rack
to be reinstalled onto the rack for the initial start-up after the
transport (see Section 5.2).
•Fig. 3a: Plenum assembly [5] with plenum for downflow blower [24] and
plenum for exhaust air blower [2]. The downflow filter and the exhaust air filter
are installed directly to the pertaining blower. The exhaust air is released into
the environment around the device through the opening [1].
•At the side of the plenum, the optional media supply lines [25] are routed into
the sample chamber [20].
•Electrical box [4] with power supply cable [3]. An RS 232 connection to a PC
and two fuse holders are installed at the front of the electrical box.
•Front cover [6] with integral, electrically movable front window [7], operated by:
•the pilot switch [26],
•the remote control [28].
The gas struts [22] secure the front cover in the open state.
Optionally, two safety latches [27] can be installed to the front cover so that
the door can be protected against unauthorized opening.
•The light dome [23] with two tubes is part of the front cover assy.
•Side slates [9] with two sealed access openings [19]. These can be equipped with media valves [13].
•Internal outlets [10] for power supply of accessories and adapter [11] for mobile
UV device.
•Optional UV lamp unit [8] consisting of two UV lamps per side.
•Optional stands [12], adjustable in height (KFS 2) and with fixed height
(KFS 1).
•Workplate segments [15] with optional arm rests [16]. A one-piece workplate
and special workplates are available options.
•Lockable drain valve [17] for installation into floorpan.
•Display [21] with remote control sensor and alarm system LEDs.
•Test hoses for the supply unit [18] at the left side of the sample chamber and
for the exhaust unit [14] at the right side of the sample chamber.
14
NOTE – Test hoses!
Do not remove the two test hoses for checking
downflow and exhaust air.
• Fig. 3b: Rack [5] with integral prefilter housing [6].
• Filter inserts [2] for the prefilter housing. The number of supplied filter inserts depends on the width of the device. To protect from liquid residues,
the filter inserts are oriented slightly sloping from the device backpanel to
form a drain duct to the floorpan [3].
The filter plates [4] have tongues and grooves and are inserted in an overlapping pattern from right to left.
•Membrane sleeve or opening for filling with aerosole for testing the filter of
the downflow unit [1] at the left side of the sample chamber and of the exhaust air unit [7] at the right side of the sample chamber.
16
Overall view / Version KSP
Fig. 3b
Page 17
4.Unit description
4.2Safety system
The safety system comprises a combination of protective and alarm systems
that ensure maximum personal and material protection.
Safety systems:
•Vacuum-sealed air system
A vacuum-sealed air system in combination with HEPA filters for downflow
and exhaust air forms the basis of the safety system for personal and material protection.
•Personal protection
Air aspired from the exterior along the entire working opening at a constant
high velocity prevents that:
• agents may leak through the working opening of the chamber.
As the exterior air pressure around the unit exceeds the pressure of the internal air system (vacuum sealing), it is ensured that:
• agents cannot be released to the exterior in the case of a leak in the
cabinet housing.
•Material protection
A steady airflow within the air system ensures that:
•a constant downflow allows the HEPA filters to remove contaminants
so that the samples are always surrounded by ultrapure air,
•harmful particles are not carried over through the sample chamber
(protection from cross-contamination).
•HEPA filters
The downflow (i.e. the air circulating within the device) and the exhaust air
(air that is released to the exterior) are cleaned by HEPA filters (HEPA = High
Efficiency Particulate Air Filter).
For version KSP, a prefiltering system is used to increase the filter efficiency and to protect the exhaust air and downflow systems.
•Safety lockout
To protect from UV radiation, the optional UV disinfection routine can be run
only if the front opening is closed. During UV disinfection, the front opening
safety lockout is activated and prevents harmful UV radiation from being
emitted from the sample chamber.
Airflow monitoring determines the velocity of the airflow in the sample chamber
as well as the inflow velocity of the air aspired from the exterior through the
working opening. As soon as airflow velocities move above or below a specified safety value, a signal is transmitted to the alarm system.
•Visual and audible alarm system
The warning system constantly monitors the safety-relevant device functions:
•Inflow velocity of the air aspired from the exterior,
•downflow velocity,
•working position of the front window.
If the warning system detects changes to one of these device functions, it
issues:
•Position monitoring
The position sensors monitor the position of the front cover as well as the movement of the front window; it will indicate when the front window is in the working position.
•Kendro Performance Factor
The Kendro Performance Factor (PER) is a value that indicates the safety state of the safety cabinet. This value is
calculated from data determined by the safety system and
from values captured empirically by service personnel during
safety checks. This data is entered into a parameter list of
the control software and interconnected. The result can be
indicated by the display.
4.3Filter system
Version KS:
Fig. 4: The filter system consists of two HEPA filters [2] and
[5] for the circulating air and for the exhaust air and of a coarse
filter for the aspired air.
HEPA filters: Room air [10] is drawn into the sample chamber through the working opening. In the air duct, room air and
the downflow within the chamber [7] are then blended to make
up the blend air [9]. The blend air is then:
•filtered proportionally by the downflow filter [5] and supplied as ultrapure air [6] evenly into the sample chamber of
the device,
•filtered by the exhaust air filter [2] and released as ultrapure air [1] to the exterior of the device.
Inlet air protection: The air duct between the sample chamber
and the device plenum has an inlet air protection [8] below the
working surface to prevent coarse particles from entering the
plenum where they may impair blower [3] und [4] and filter
functions.
Filter system with prefilters,
Fig. 4a
downflow filter and exhaust air
filter / Version KS
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Page 19
4.Unit description
Version KSP:
Fig. 4b: The filter system consists of a set of HEPA prefilters
[10] (the number of filters depends on the sample chamber
width) and two HEPA filters [2] and [5] for downflow air and
exhaust air.
HEPA prefilters:
The compact size of the filter inserts allows filter replacement
with minimal contamination hazard. The use of the prefilters
protects the downflow and exhaust air filters considerably.
The filter inserts used are HEPA filters of Class H14 (according to DIN EN 1822). Alternatively, activated carbon filters can
be inserted into the housing to protect from smells.
Room air [11] is drawn into the sample chamber through the
working opening. In the air duct above the prefilter, room air
and the downflow within the chamber [7] are then blended to
make up the blend air [8]. The blend air is then:
•aspirated into the prefilter [10], filtered and fed into the air
duct [9].
Fig. 5: The safety cabinet is equipped with two separate control
elements that operate independently of each other:
•remote control [3],
•pilot switch [1].
The status indicators of the display [2] indicate control operations initiated with the control elements.
Remote control: All device functions can be activated and
deactivated easily using the remote control.
Pilot switch: Use to control all basic functions required for the
operation. This switch allows you to operate the safety cabinet if the remote control is not operational or available.
Fig. 6: The display [1] can show text or numeric values and
has 12 LEDs [2] and [4] to indicate the current operational state
of the unit.
The display module also houses the sensor system for the remote control. The pulses transmitted are best received by the
sensor if the distance A between the remote control [5] and the
sensor [3] does not exceed 1m and if the radio signal beam
does not deviate more than 15° horizontally.
The transmission range of the remote control also depends on
the battery state of charge.
Fig. 5
Controls and display
20
Display with remote control
Fig. 6
sensor
Page 21
4.Unit description
4.5Sample chamber access
Front cover:
The sample chamber of the device is accessible via two modes:
Fig. 7a: Manual opening of the front cover [2] allows access
to the complete sample chamber width with an opening height
C. It is generally needed for decontamination and introduction
of larger accessories.
NOTE – Front cover lockout!
The front cover is equipped with a safety lockout and can only be opened in standby mode,
i.e. when the front window is completely
closed.
As an option, the front cover of both versions can be equipped with a safety feature: Two safety latches at the left and right
frame struts secure the front cover from unauthorized opening.
With this equipment, the function of the pilot switch is overridden
by a safety bezel so that only the remote control can be used
to access the sample chamber.
If the remote control is faulty, the safety bezel can be unscrewed
and the device can be operated using the pilot switch (see Section 6.4).
Front window:
Fig. 7b: The electrically operated front window [1] is made of
multi-layer safety glass and integral to the front cover frame.
It can be raised to a maximum opening height B. To access
the sample chamber during the work process, the front window must stay in the work position with opening height A.
CAUTION – Front window movement!
Do not attempt to move the front window
manually as otherwise the motor drive may be
damaged.
Access through front cover
Fig. 7a
Lowering the front window when the device is deenergized:
The safety feature (optional) is backed up by a battery. Should
a power failure occur, the pilot switch (see Section 6.4) can be
used to lower the front window completely.
Fig. 8: The standard equipment includes the outlets [10] for internal power supply
as well as the openings [8] on both sides for routing of cables and hoses. All other
supply connections are available as options and may also be retrofitted.
Power supply connection: The connection to the power supply system is achieved through a cable with grounding plug [2] at the rear of the electrical box.
Contact connection: The front of the box has an RS 232 interface [3] for the
connection to a PC as well as two fuse holders for 5A miniature fuses:
[4] for (L),
[5] for (N).
Internal power supply: There are two electrical outlets (5A) [10] and one UV
disinfection adapter (optional) [9] located in the side walls.
Media valves: There are two sealed feed throughs [8] on each side. These may
be used for installation of media valves [7].
Media supply lines: Additionally, media can be supplied into the sample chamber
through three pipes. The inlets [1] are located on top of the housing, the outlets [6] are placed at the sample chamber backpanel.
22
Supply interfaces
Fig. 8
Page 23
4.Unit description
Disinfection adapter (optional): The disinfection adapter [9] is used to con-
nect a mobile UV radiation device. The adapter is connected to the device control,
the UV disinfection routine with a mobile UV device may be controlled with the
remote control.
Media connections (optional): The media supply unit consists of three pipes
that are routed into the sample chamber through the top of the unit. The inlet
connections [1] and outlet connections [6] with thread (R 3/8"), e.g. for media
valves [7], are preinstalled and equipped with a sealing plug.
The media connections are universal-type connections. Two equipotential bonding
connections are installed at the top of the unit and at the stand.
External systems: A failure detection systems or gas supply solenoid valves
may be connected to the safety cabinet control. The unit may also be connected to an external ventilation system. These external systems can then be enabled or disabled in the secured operating mode using the remote control.
Caution – Combustible gas!
If a gas burner is to be operated in the sample chamber, an appropriate shut-off device for
the gas supply system (shut-off valve, solenoid
valve) must be installed.
Use only laboratory safety burners in the sample chamber.
Fig. 9: The UV lamp unit consists of two lamp housings [2] with
two UV lamps each [1] that are integral to the side walls. Both
lamp housings are protected by a stainless steel cover [3].
By cross-radiation of the UV units, all surfaces will be disinfected
as the shadow zone is reduced.
The operating time of the UV lamps is preset. The UV disinfection routine can be activated quickly by using the remote
control.
NOTE – Protection from UV radiation!
As a protection from harmful UV radiation, the
UV lamps can only be activated if the front
window is completely closed.
4.8Working area
The standard equipment consists of the segmented workplates.
One-piece or special workplates are available as options.
The workplates or workplate segments are placed onto the
frame above the sample chamber floorpan using two submerging wire straps as handles.
Fig. 10: The working area A for perfect material protection extends over the entire width B and depth C of the workplate.
The two arm rests [3] are positioned at a distance
D (20 cm) to each other centrically on the workplate [1] or on
the workplate segments. The armrests are installed to the
second perforation line [2] of the workplate.
Fig. 9
UV lamp unit
24
Working area on the workplate,
Fig. 10
armrests
Page 25
5.Start-up
5.1Initial operation
Prior to initial operation, the safety cabinet must be subjected
to an installation test. Correct assembly and installation performed by the operator are essential for good start-up.
Version KSP:
Upon the initial connection to the power supply system, the
device control of version KSP starts an automatic calibration
routine to determine the parameters for the safety system of
the device.
The calibration routine is no substitite for the
installation check performed by the service
personnel.
5.2Installing unit and accessories
Version KS:
Device without rack:
•Place the device without rack onto a sufficiently stable
substructure so that the weight of the device frame does
not rest upon the floorpan.
•Remove the protective foil from the floorpan.
Device with rack:
Assemble the rack (accessory) and install the device frame onto
the rack:
1. Fig. 11a: Install the two side members [3] to the rear support
[4] using the screws [5].
2. Place the safety cabinet on the stand with the guide pins
[1] positioned properly in their receptacles in the stand.
3. Install the housing to the vertical posts of the stand with the
retaining screws [8].
4. Align the safety cabinet working area. Place a bubble level onto the workplate and use a wrench (size 24 mm) to
rotate the rack stands [6] so that the workplate is perfectly horizontal in all directions. When adjusting the rack stand
height, proceed from left to right and from rear to front.
Version KSP:
Fig. 11b: The rack [3] and the prefilter housing [2] form an as-
sembly. To facilitate the installation of the device frame [1],
the rack is equipped with a lifting mechanism [5].
The four columns [7] of the rack have threaded rods [6] onto
which the device frame can be placed and lowered.
NOTE – Installation
The cabinet must be installed only by authorized service personnel.
NOTE – Leveling the device
To level the device, do not use the lifting mechanism but only the adjustable stands of the
device.
Align the working area of the safety cabinet:
Place a bubble level onto the workplate and rotate the stands
[4] of the rack using a 24-mm wrench until the workplate is
exactly horizontal in all directions. For the vertical alignment
of the device stands, proceed from left to right and from rear
to front.
Installation / Version KSP
Fig. 11b
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Page 27
5.Start-up
Drain valve (optional)
Fig. 11c: The drain valve [2] is installed into the floorpan ope-
ning [1] at the floor at the left front area of the sample chamber.
The cabinet should be levelled only after it has been
positioned.
1. Remove transport protection (foil) from the workplate or from
the workplate segments.
2. Lift the workplate or the workplate segments by the wire
hooks and place it/them onto the front and rear rails in the
sample chamber with the wide line of holes facing forward.
3. Place a bubble level onto the workplate and use the four
levellers to effect a level state in all planes.
5.4Activating the remote control
The remote control operates on two batteries with the following
specifications:
•1.5 V alkaline cell (AAA,Type LR 03)
Installing the batteries:
1. Fig. 12: Open the lid [4] of the battery housing at the bottom of the remote control by inserting a pointed instrument
into the notch [1] and prying the lid off.
2. Insert the batteries [3]. The positive and negative poles are
marked at the bottom of the battery housing.
3. Check the position of the coding switch [2]. If the switch is
not set to position 1, rotate it to that position.
4. Insert the two hinges of the lid into the joints at the battery housing and slightly press onto the lid so that the retaining clip engages.
Functional check:
After the safety cabinet has been connected to the power supply
system, the function of the remote control can be checked by
switching on the light within the sample chamber. Point the remote control toward the display at the sample chamber backpanel:
Press key
Contamination protection:
While the sample chamber is used, protect the remote control against dirt and contamination by using the disposable
transparent cover.
Inserting the batteries
Fig. 12
28
Page 29
5.Start-up
5.5Power supply connection
WARNING – High voltage!
Contact with current-carrying components may
cause a lethal electric shock.
Before connecting the device to the power supply system, check plug and power supply cable
for possible damage.
Do not use damaged components to connect
the device to the power supply system!
Establishing the power supply connection:
1. Before connecting the device to the power supply system, check to see if the
voltage of the outlet corresponds with the specifications on the nameplate
of the device. If the ratings given for voltage (V) and maximum current (A)
are not correct, the device must not be connected to the power supply
system.
2. Connect the grounding plug of the device to a properly grounded and fused
outlet.
•The outlet must be fused separately using a fusible link T 16 A or using
a circuit breaker B 16.
3. Make sure that the power supply line is not subjected to tensile or compressive
force.
The mains power supply outlets should be out of reach to prevent accidental
shutting off. Ideally, the outlets should be installed above the safety cabinet.
Connecting the equipotential bonding:
If the sample chamber is supplied with media (gas, water, etc.), the on-site equipotential bonding must be connected to one of the premounted threaded bushings
either at the top of the housing or at the stand.
Initialization routine:
After the unit has been connected to the power supply system, the device control
runs through a start-up initialization routine and switches the functions to the OFF
mode. The safety cabinet is now operational and can be operated using the remote control or the pilot switch
Presetting the alarm limits upon initial operation, KSP:
NOTE – Presetting the alarm linits!
The alarm limits must be set only upon the
initial operation of the device.
After the initialization routine has been completed, the calibration routine is run:
•The display shows "CAL".
•The routine starts automatically when the device control is in the work mode
(sees Section 6.2) and runs for approx 30 minutes. If the airflow is disturbed
during the run time, the routine is cancelled and restarted automatically.
•At the end of the routine, the determined parameters for the alarm limits are
saved.
NOTE – Initial operation!
According to applicable national standards
and regulations, the calibration routine is no
substitute for a start-up performed by an authorized service technician.
Setting the clock:
After the initialization routine has been run, the clock should be set to the appropriate time zone (see Section 6, Operation).
NOTE – Power supply connection!
The safety cabinet should remain connected
to the power supply system at all times to ensure that settings for the individual unit configuration remain active in the memory. If the
power supply is interrupted for more than 5 minutes, the time must be reset correctly.
After the power supply connection has been
reestablished, the system switches to the operating mode that had been active last.
30
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5.Start-up
5.6RS 232 interface connection
The RS 232 interface has been designed for a cable connection
with 9-pin connectors and a contact assignment of 1:1.
Connection of the device:
1. Turn PC off.
2. Fig. 13: Connect the connector of the serial interface cable (not comprised in the scope of delivery) to the
socket [1] at the supply interface at the front of the electrical box.
3. Connect the serial interface cable to an unassigned slot
COM 1/COM 2 etc. at the PC.
4. Turn PC on.
Transfer protocol:
The interface must be configured as follows:
Baud:9600
Data bits:8
Parity:none
Stop bit:1
Protocol:none
FIFO–puffer(extended modulation):enabled
Do not operate the device before the installation test has been completed.
•The installation test must be performed in accordance with the specifications of EN 12469 / 2000. The cabinet may be operated as a Class II microbiological safety cabinet, in accordance with EN 12469 / 2000, if the device
functions or function patterns listed below were checked and if the test results are within the safety value tolerances specified in Annex F:
•Electrical safety test
•Inflow velocity test
•Downflow velocity test
•Leakage test of HEPA filters
•Airflow control test
•A repeat test must also be performed after repairs to the device or after considerable changes (more than 5 cm) to the location of the device.
•The operator must prepare a test report or request a written test report from
the authorized test service.
NOTE – Safety warranty!
The operational safety of the device, particularly the personal and material protection, are
guaranteed only if all safety functions of the
device have been tested and approved.
Kendro Laboratory Products will not warrant
the operational safety if the device is operated
without performance of the required installation test or if the installation test and repeat test
are not performed by adequately trained and
authorized personnel!
NOTE – Device hygiene!
The initial start-up with subsequent installation
test does not include any decontamination
measures. For operation in the work process,
the sample chamber of the device and the
accessories required for the work process
must be disinfected and cleaned in accordance
with the hygiene guidelines set forth for the
application.
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6.Handling and control
6.1Display
Fig. 15: The display at the sample chamber backpanel shows
•status messages,
•parameter input and putput.
6.1.1Functions of the display components
[1] Display segment for numbers and text
Value displays
The LEDs 2-5 illuminate when the corresponding value is called up:
[2] Display time (yellow LED), usually the active standard display
[3] Display downflow velocity (yellow LED)
[4] Operating hours after last filter replacement (yellow LED)
[5] Display Kendro Performance Factor (yellow LED)
Function displays
The LEDs 6-9 illuminate only when the pertaining value is called up:
[6] Ventilation reduced (yellow LED)
[7] Potential-free contact activated (yellow LED)
[8] Internal power supply activated (yellow LED)
[9] UV disinfection routine activated (yellow LED)
The LEDs 10/13 and 11/12 show the operating condition of the device as either/or conditions:
[10] Front window is not in working position (red LED)
[13] Front window is in working position (green LED)
[12] Airflow is steady (green LED)
[11] Airflow is not steady (red LED)
The routine runs for approx 30 minutes. During this time, the display shows
alternatingly cal and the descending time value. If the calibration routine cannot be started due to faults, the display shows cal permanently.
6.1.3Display in OFF mode
In the OFF mode, the display shows the current time.
For the initial start-up of the device, the clock must be set to the correct time zone
and to the corresponding time output (CET mode or AM/PM mode)
(see Section 6.3.9).
6.1.4Display in work mode
In the work mode, the display shows the values of the device data that had been
shown last (see Section 6.3.9.ff):
•Time (hours and minutes)
•Downflow velocity
•Operating hours after last filter replacement
•Kendro Performance Factor
6.1.5Power interruption
If the master PCB of the device detects a power failure or a failure of the power supply, a warning is issued.
Note – Power failure warning
In case of a power failure, the display goes off
after 10 seconds, then an audible alarm signal
sounds for about 30 seconds.
After this warning, the device is no longer
operative.
The safety feature (optional) allows the complete lowering of the front window
after a power failure by pressing the pilot switch.
6.1.6Display and functions after a power failure
After the power supply has been reestablished after a power failure, the display
shows the values and functions that were last shown prior to the failure.
The functions that had been selected last will be continued where they had been
interrupted.
6.1.7Failure messages
Failure messages are shown on the display as text/number combinations with
the codes ER 1 to ER 6. If one of these codes appears on the display, contact
Technical Service immediately.
34
Error codeFault cause
ER 1Pressure sensor 1 / supply
ER 2Pressure sensor 2 / exhaust
ER 4BUS error
ER 5RAM error
ER 6Fernbedienung defekt
Page 35
6.Handling and control
6.2Description of the operating modes
The following operating modes exist for the device:
•Calibration mode (after initial start-up, version KSP)
•OFF mode
•Work mode
•Standby mode
•UV mode
OFF mode: The device is at "idle". Utilized for charging the sample chamber
or for cleaning and disinfection, the front cover can be opened when the front
window is completely closed.
•The air system blowers are switched off.
•The sample chamber illumination is available.
•The internal power supply within the sample chamber is available:
•If the internal power supply is activated, the yellow status indicator IN-
TERNAL POWER SUPPLY ACTIVATED
•The display shows the time. If the timer has been activated, the display alternately shows the current time and the preset switching time for the timer.
•The contact for the external device connection is not available.
Work mode: Ensures personal and material protection. In this operating mode,
the work process is run within the sample chamber. The device is in the work
mode when the front window has been moved into the working position and the
airflow is steady.
•The front window is in the working position:
•The green status indicator
FRONT WINDOW IS IN WORKING POSITION is
illuminated.
•No audible alarm signal.
•The air system blowers are switched on to ensure steady airflow:
•The green status indicator
AIRFLOW STEADY is illuminated.
•The sample chamber illumination is available.
•The power supply for the sample chamber outlets is available:
•If the internal power supply is ON, the yellow status indicator
POWER SUPPLY ACTIVATED
is illuminated.
INTERNAL
•The monitor-contact for the external device connection is available:
•If the contact is activated, the yellow status indicator
CONTACT ACTIVATED
is illuminated.
POTENTIAL-FREE
Standby mode: For an interruption of the work process, the front window can
be lowered and the sample chamber sealed aerosol-tight. The air system output has been reduced to match the lower air requirement.
•The front window is closed:
•The air system operates at reduced output.
•The red status indicator
FRONT WINDOW IS NOT IN WORKING POSITON is
illuminated.
•The yellow status indicator
AIRFLOW REDUCED is illuminated.
•The sample chamber illumination is available.
•The internal power supply in the sample chamber is available:
•If the internal power supply is ON, the yellow status indicator
UV mode: For running the UV disinfection routine, the front window is completely
lowered to protect against UV radiation. The routine cannot be run until the front
window is in the "closed" position.
•The UV disinfection routine is activated:
•The yellow status indicator
minated until the preset time for the routine has elapsed. Then, the UV
lamps are switched off automatically, and the status indicator is switched off.
•The sample chamber illumination is available.
•The internal power supply in the sample chamber is not available.
•The integral UV lamp (optional) is available.
•The power supply for the UV disinfection adapter (optional) is available.
UV DISINFECTION ROUTINE ACTIVATEDis illu-
36
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6.Handling and control
6.3Remote control
The device control software monitors the selected operating
mode of the safety cabinet and automatically deactivates operating functions that are not in accordance with the safety requirements of the mode.
The remote control has priority over the pilot switch.
6.3.1Basic functions
Fig.16: The simple operation of the remote control allows the
user to control all basic functions using a minimum of operating steps to switch the safety cabinet into work mode.
When the front window reaches the working position, the movement stops automatically. When the
movement starts above the working position, the
front window stops at the maximal opening position.
Stopping the upward movement:
Press, then release the key
This function can be selected with any key except
the key.
Lowering the front window:
Keep the key depressed
Stopping the downward movement:
Release the key
Basic functions of the remote
Fig. 16
control
Silencing the audible alarm signal, (see section 6.3.3):
Availability of functions in the different operating modes: x = available
Key
/$
OFF modeWork mode
XXXX
XXX
XXXX
XXXX
XX
Mode
Standby
mode
XX
XX
XX X
X
6.3.2Moving the front window to the working position
UV mode
1. Raising or lowering the dront window:
Press, then release the key for approx. 1 second
The red status indicator on the display is illuminated
The audible alarm signal is on.
2. When the front window reaches the working position, the movement is automatically stopped.
The green status indicator on the display is illuminated
The audible alarm signal is off if the airflow is steady.
3. If the movement starts above the working position, the front window must first
be lowered below the working position and then be raised again. To lower
the front window:
Keep the key depressed
4. To stop the downward movement:
Release the key
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6.Handling and control
6.3.3Silencing the audible alarm signal
When the front window is moved out of the working position or when the pressure sensors detect a safety-relevant change of the airflow velocities, the corresponding visual and audible alarm signals are issued. To silence the audible
alarm:
•Press, then release the key
NOTE – Quitting the optical alarm signal!
The optical alarm signals can notbe quitted.
They change her status indication only, if the
necessary functions of the device are
operational.
The audible alarm signal only can be silenced,
if the front window is either completely closed
or has been moved to the maximum upper opening position.
In the working position the audible alarm signal
can not be silenced.
Changing the factory setting:
You can change the factory setting so that the audible alarm signals can be
silenced (switched off) in any operating mode at any position of the front
window. The status is at the display as follows:
•P7 0signal can be silenced
•P7 1signal cannot be silenced
Switch the device to OFF mode:
•Keep the key depressed until the ready signal sounds
•Keep the key depressed until the ready signal sounds
Activate the factory settings:
•Keep the key depressed for 5 seconds
6.3.4Switching the illumination on and off
In each operating mode, the sample chamber illumination can be switched on
or off.
1. To switch the illumination on or off:
Press, then release the key
6.3.5Activating and deactivating the internal power supply
All outlets in the sample chamber can be activated (power supply on) or deactivated (power supply off) simultaneously.
1. To activate the power supply:
Press, then release the key
The yellow status indicator is illuminated.
2. To deactivate the power supply:
Press, then release the key
The yellow status indicator goes off.
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6.Handling and control
6.3.6Displaying the UV disinfection time
This value refers to the operating hours of the set run time of the UV disinfection or of the power supply for the UV disinfection adapter (optional). This display function is only available when the front window is not closed. The device
must be switched to work mode.
1. To display the value:
• Press, then release the key
The time value is output in segments of 30 minutes.
2. To deactivate the value display:
The value display is deactivated automatically after 2-3 seconds.
6.3.7Activating and deactivating the potential-free contact (optional)
An external solenoid valve or alarm system that is connected to the safety cabinet control system can generally be enabled only if the device is operated in
the secure work mode. Enable or disable an external system:
The current time of the time zone in which the unit operates must be set at the
start-up of the safety cabinet. Two different display modes can be selected:
•CET mode (24:00 hours)
•AM/PM mode (12:00 hours)
When the time is to be set, the device must be in the OFF mode.
1. Set the time:
Keep the . key depressed until the two-digit hour disply flashes.
The minute display shows either A, P or no value (CET time display).
The time zone is set at the same time as the hour value: First, set
the time zone (sequence: CET, A, P), then set the exact hour value.
2. While increasing or decreasing the hour value in increments, set the
time zone:
Press, then release the or the key
3. Scroll through values:
Keep the or the key depressed
If the keys are depressed for approx. 2 or 3 seconds, a higher scroll
speed is selected.
4. Store the hour and time zone setting:
Press the . key
The function switches to minute display (flashing).
5. Set the minutes:
Press, then release the or the key
6. Scroll through values:
Keep the or the key depressed
If the keys are depressed for approx. 2 or 3 seconds, a higher scroll
speed is selected.
42
7. Store the minute value:
Press, then release the . key
The display shows the time.
Page 43
6.Handling and control
NOTE – Calling up device data!
The following data can be called up in
succession:
• Time (hours/minutes)
• Downflow velocity
• Operating hours after last filter
replacement
• Kendro Performance Faktor
To call up values in succession:
• Press, then release the . key for each
value
The following three sections contain detailed
information about displaying values.
The sensor system of the device continuously monitors the downflow velocity
of the airstream in the sample chamber. The currently determined value (m/s)
can only be called up in the work mode.
•To display the velocity value:
Press the . key repeatedly until the yellow E LED illuminates.
6.3.11Displaying the operating hours of the HEPA filters
The filter total operating hours after the last filter replacement can be displayed. Upon each filter replacement, the hours are reset to zero.
1. To display the total operating hours:
Press the key repeatedly, until the yellow status indicator
The Kendro Performance Factor (PER) is a value that indicates the safety state
of the safety cabinet.
This value is calculated from data determined by the cabinet safety system and
from values captured empirically by service personnel during safety checks. This
data is entered into a parameter list of the control software and interconnected.
The result can be indicated by the display.
1. To call up the PER:
Press the . key repeatedly until the yellow status indicator
on the display is illuminated.
The PER is displayed as an integer.
2. To evaluate the PER:
Number in the 100 to 60 range: The safety cabinet is operationally
safe. Personal and material protection is ensured.
Number in the 59 to 30 range: The safety cabinet is still operationally safe. Personal and material protection is ensured.The safety
system should be checked.
Number in the 29 to 0 range: The safety of the device may be impaired. The failure causes must only be repaired by authorized service personnel. Contact Technical Service.
NOTE – Calling up value!
The value should only be called up, when the
airflows have stabilized itself after a lead time
of approx. 20 min.
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6.Handling and control
6.3.13Setting and activating the timer
The timer function allows you to switch the safety cabinet from the OFF mode
to the work mode at a predetermined time. The timer can only be set when the
device is in the OFF mode.
1. To set the switching time:
Keep the key depressed until the ready signal sounds
The display flashes the two-digit hour display.
2. Increase or decrease the hour value in increments:
If the timer is to be activated without a prior
change of the time values, the procedure above is performed and the existing time values
are confirmed:
Press, then release the . key for each
value.
After the minute value has been confirmed, the
timer has been activated.
NOTE – Timer function!
The timer can not be used as a start routine
that calls up its function automatically.
It must be reactivated separately for each deferred device start.
6.3.14Deactivating the timer
If the device was started at a preset time, the timer function is also deactivated
automatically. The deferred start can therefore be cancelled only while the device is still in the OFF mode.
•To deactivate the timer function for starting the device:
Keep the key depressed until the ready signal sounds
The safety cabinet changes to the work mode.
The status indicator illuminates continuously, and the display
shows the current time.
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6.Handling and control
6.3.15Setting the UV disinfection time
Depending on the equipment option of the cabinet, this setting is used to:
•determine the disinfection time of the optional UV lamps in the side walls or
•set the time for the power supply of a mobile UV device at the UV adapter.
Factory setting is one hour. The time can be set within a range between 0 and
24 hours in increments of 30 minutes each. The unit must be in the work mode
(the front window must not be closed). For each following start of the UV disinfection, the routine is run with this preset time value.
1. Select the function:
Keep the key depressed until the ready signal sounds
The display flashes the run time that had been selected last.
2. Set or change the disinfection time. To increase the value in increments:
3. Scroll through the value display in increments of 30 minutes:
Keep the or the key depressed
4. Store the setting:
Press, then release the . key
If the setting is not stored, the disinfection time will be reset to the original value after approx. 15 seconds.
6.3.16Starting the UV disinfection
The UV disinfection can only be started if the front window is completely lowered
(standby mode). Depending on the equipment of the unit version, this function
is used to:
•switch on the optional UV lamps in the side walls or
•activate the power supply for the outlets of the mobile UV device.
•Start the routine:
Keep the key depressed until the ready signal sounds
The display alternately shows the Text
fection time in hours and minutes.
The yellow status indicator is illuminated.
After the disinfection time has elapsed, the current time is displayed.
While the UV disinfection routine is run, it can be interrupted at any time.
1. Cancelling the routine:
•Press, then release the key
The status indicator goes off.
2. The display shows the current time.
6.3.18Activating the stop watch
The stop watch function starts a countdown for a preset time (max. 99 min and
59 s) and issues an audible signal when the set time has elapsed. The signal
cannot sound if some other device function has already caused an alarm. The
function can only be avtivated if the device is in the work mode.
1. Select the stop watch function:
Keep the key depressed until the ready signal sounds
2. Set the minutes (0 - 99) in increments:
Press, then release the or the key
3. Scroll through the value display:
Keep the or the depressed
If the keys are depressed for approx. 2 or 3 seconds, the higher
scroll speed is selected.
4. Store the minute setting:
Press, then release the . key
The function switches to second display (flashing).
5. Set the second value (0 - 59):
Press, then release the or the key
48
6. Scroll through the value display:
Keep the or the depressed
If the keys are depressed for approx. 2 or 3 seconds, the higher
scroll speed is selected.
7. Store the setting and start the stop watch:
Press, then release the . key
Page 49
6.Handling and control
8. Indication of the function:
The predefined time value counts to zero.
6.4Pilot switch
Fig. 17: If the remote control is not available, the basic func-
tions required for the operation of the device can be controlled
with the pilot switch:
•switch the device on,
•Raising and lowering the front window,
•silencing the audible alarm signal,
•switching the device to OFF mode.
6.4.1Moving the front window:
The movement of the front window is controlled by depressing
the pilot switch control element with the corresponding arrow
symbol.
1. To raise the window, press the control element [1]. When
the front window reaches the working position, the movement stops automatically. If the movement starts above the
working position, the front window stops at the maximal
opening position.
2. To stop the upward movement, release the control element.
3. To lower the window, keep the control element [2]
depressed.
4. To stop the downward movement, release the control
element.
If the device is started with the pilot switch, the
chamber illumination is switched on.
If the device is switched off with the pilot
switch, the chamber illumination is also switched off.
5. If the front window is not in the working position,
•the red status indicator
KING POSITION
is illuminated.
FRONT WINDOW IS NOT IN WOR-
•the audible alarm signal can be silenced (i.e. swit-
ched off) if the front window is either completely
closed or has been moved to the maximum upper
opening position.
6. When the front window reaches its working position, the
movement stops automatically:
1. Move the front window to the upper and lower end positions.
2. Release the control element.
3. Press, then release the control element. The audible alarm
signal remains off.
6.4.3Switching the device to OFF mode
1. Move the front window to the upper and lower end positions.
2. Release the control element.
3. Keep the previously actuated control element depressed until the ready signal sounds.
NOTE – Switch-off function
If the device is switched to the OFF mode with
the pilot switch, the chamber illumination is
switched off. The device-internal power supply remains in the last functional state that it
had been switched to.
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7.Operation
7.1Hygiene preparations for the sample chamber
The sample chamber surfaces and the accessories required for the work process must be disinfected and cleaned in accordance with the hygiene guidelines set forth for the application.
7.2Preparing the sample chamber
Installing the accessories:
1. Lower the front window completely.
2. Open the front cover or move the front window into the maximum opening
position.
3. Position the accessories in the working area of the workplate.
4. Close the front cover.
5. Move the front window to its working position and wait until the airflow has
stabilized.
The personal and material protection is ensured only if the airflow system of the device is
working properly.
If the alarm system issues failure messages
when the front window is in the working position, stop all applications that may release
harmful aerosols!
NOTE – Application of cystostatic agents
For the application of cystostatic agents, the
lowered single-part working plate must be
used.Application of cystostatic agents
6. Then place samples into the chamber.
7. To interrupt the work process or for extensive experiment cycles without
manual interference, switch the device to standby mode. When the working
opening is completely closed, the safety cabinet is sealed aerosol-tight.
Failure messages are displayed in form of text/number combinations with a code
between ER 1 and ER 6 (see chapter 6.1.6). If one of these codes is displayed, contact the Technical Service immediately.
To isolate the cause of the failure, the operating personnel must perform only
the following tests and measures:
•Check to see if the exhaust air opening on top of the cabinet is blocked.
•Ensure that the on-site exhaust air system is activated.
•Close doors and windows in the laboratory to prevent drafts.
•Switch off devices in the vicinity of the safety cabinet that cause air turbulence or emit excessive heat.
•Open flames in the sample chamber may impair airflow conditions.
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7.Operation
7.4Work rules
The observance of work rules ensures a minimum of operational safety when
handling the safety cabinet.
Before starting an operation:
•Take off jewelry.
•Put on required personal protective gear, e.g. hand, face, or body protection.
•Clean and disinfect sample chamber surfaces at regular intervals.
During operationan:
•Place samples only with in the defined work area of the workplate.
•Do not place unnecessery items into the sample chamber.
•Use only disinfected and cleaned accessories for the work process.
•Do not cause air turbulence, by quick hand, arm or body movement in the
sample chamber or in front of the work opening.
•Do not place accessories into the sample chamber that cause air turbulence
or emit excessive heat.
•Do not block air circulation at the ventilation slots of the workplate.
Sitting posture during work:
Fig. 18: To prevent risks to health, a height-adjustable
working chair with an adjustable seat back should be
used during extended work periods at the safety cabinet.
A When the forearm rests on the armrest, is should
be in an almost horizontal position.
B When the thigh is in a horizontal position, the angle
between thigh and lower leg should exceed 90°.
To ensure a compensation between floor and sitting
height, a footrest (DIN 4556) should be used. The minimal effective surface of the footrest should be
45 x 35 cm.
C The slope should be adjustable within a range of
5° to 15°.
D The adjustable height should extend to a minimum
of 11 cm above the floor.
After finishing an operation:
•Remove samples from the sample chamber and
store them properly.
•Clean and disinfect the sample chamber surfaces,
including the workplate and the floorpan. Clean and
disinfect all accessories.
To interrupt a work process, the device is switched to the OFF mode.
1. Remove all samples from the safety cabinet and store them properly.
2. Remove accessories from the sample chamber and clean and disinfect them.
3. Clean and disinfect the sample chamber surfaces, the workplate, and the
floorpan.
8.2Shutting the unit down
If the unit is not to be used or stored for an extended period of time, it must be
completely decontaminated.
CAUTION – Decontamination measures!
To shut the device down, the sample chamber
must be disinfected completely and the plenum, including the filters, must be sterilized
using formaldehyde.
1. After the device has been decontaminated, close the front window completely.
2. Disconnect the device from the power supply system.
NOTE – Removing the batteries!
To prevent the batteries from leaking and to
prevent their contacts from oxidizing during extensive operation intermissions, remove the
batteries from the remote control and store
them in a dry place.
The batteries for the safety feature "Lower front
window" are leakproof and do not need to be
removed.
The nickel-metalhybrid battery for the safety
feature "Lower front window" is leakproof and
can remain installed.
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9.Cleaning and decontamination
9.1Decontamination procedure
Several procedures can be applied for decontaminating the safety cabinet. Which
procedure is selected, depends on:
•the potential risk imminent in the agents,
•the degree of purity required by an experiment or by a work process.
Possible decontamination procedures:
Wipe/spray disinfection: is the standard disinfection procedure for cabinets
used for microbiological experiments.
UV disinfection: is particularly suited as an intensifying additional disinfection
after a wipe/spray disinfection.
Sterilization with steam: can be used for treating the removable stainless steel
components. Examples of autoclavable components are the bezels of the UV
lamps, the workplate or workplate segments and the armrests.
Disinfection with formaldehyde: can be performed if a sterile scmple chamber is required for the work process. This sterilization procedure is mandatory:
•when filters are replaced,
•when the device is shut down,
•when the device is discarded.
9.2Wipe/spray disinfection
The wipe/spray disinfection is performed in three stages:
•Predisinfection,
•cleaning,
•final disinfection
Recommended disinfectants:
NOTE – Compatibility!
Chloride-containing disinfectants may damage
some surfaces; use only chloride-free disinfectants or a disinfectant with a low enough chloride content to have been proved harmless for
stainless steel finishes!
Disinfectants with an alcohol content of more
than 70 % may cause embrittlement of plastic
components after extended esposure. Use only
disinfectants with a low alcohol content. When
using a disinfectant with an alcohol content
of more than 70 %, the release limit of 200 g
within 2 hours must not be exceeded.
Also suited are disinfectants based on quaternary ammonium compounds.
1. Remove all samples from the sample chamber and store them properly.
2. Remove accessories from the safety cabinet and disinfect them using the
disinfection procedure recommended by the manufacturer.
3. The workplate and stainless steel components can be removed from the sample chamber and disinfected separately.
4. For predisinfection, spray disinfectant on all sample chamber surfaces or wipe
the surfaces using disinfectant.
5. Do not remove the optional UV lamps from the sockets; wipe them thoroughly using a damp cloth.
6. Switch the device to work mode, move front window to the working
position.
7. Allow disinfectant to react as recommended by manufacturer, then operate the safety cabinet for at least 15 to 20 minutes in the work mode so that
released aerosols can be absorbed by the filters.
Cleaning:
1. Wipe the surfaces clean using a clean cloth and plenty of clear water.
2. Remove dirt residues and deposits thoroughly.
3. Remove the cleaning liquid from the floorpan and wipe all sample chamber
surfaces dry.
Final disinfection:
1. Again, spray disinfectant on all sample chamber surfaces or wipe the surfaces clean with disinfectant.
2. Allow disinfectant to react as recommended by manufacturer.
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9.Cleaning and decontamination
9.3UV disinfection after a wipe/spray disinfection
A UV disinfection can be performed either by using the optional integral UV lamps
or by using a mobile UV device. The run time of the routine can be preset with
the remote control (see Section 6.3.16).
9.3.1UV disinfection using the integral UV lamps
1. Remove the stainless steel covers from the lamp mountings in the side walls.
2. Lower the front window completely.
3. Start the UV disinfection using the remote control.
9.3.2UV disinfection using a mobile UV device
To control the disinfection routine of a mobile UV device with the safety cabinet software, ensure that the connecting plug of the UV device is compatible with
the UV disinfection adapter of the safety cabinet.
1. Position the mobile UV device centered on the work area and connect to the
UV disinfection adapter.
2. Lower the front window completely.
3. Start the UV disinfection using the remote control.
For gas disinfection, formaldehyde is evaporated in the tightly sealed sample
chamber. The quantity of the formaldehyde used depends on the sample chamber
volume of the cabinet version to be disinfected (see Technical Data).
Per cubic meter of sample chamber volume, at least 5 g formaldehyde must be
evaporated with 20 ml water (corresponds with 25 ml of a 20 % formaldehyde
solution). The formaldehyde evaporates immediately when its boiling point is
reached. The required reaction time is at least 6 hours.
After the required reaction time, the formaldehyde should be neutralized by evaporating a 25 % ammonium solution (10 ml per cubic meter of sample chamber volume).
Ambient conditions and accessories:
The temperature at the cabinet location should be approx 21° C, the relative
humidity should be between 60 and 85 %. To evaporate the solution, a heating
device with a container is required.
NOTE – Disinfection procedure!
A disinfection with formaldehyde must be
performed in accordance with the specifications of NSF 49/1992, Annex G.
As this procedure has considerable risks, it
must only be performed by specially trained
and authorized service personnel!
Wipe the exterior surfaces of the device clean using a solution of tepid water
and commercial dishwasher solution. Then, wipe exterior surfaces dry using a
soft, clean cloth.
9.6Cleaning the floorpan
Clean the floorpan using a solution of tepid water and commercial dishwasher.
1. Remove the working plates from the sample chamber.
2. Remove dirt residues and deposits thoroughly.
3. Wipe the floorpan clean using a clean cloth and plenty of clear water.
4. Remove the cleaning liquid from the floorpan and wipe the floorpan surfaces
thoroughly clean.
NOTE – Material residues
After cleaning, make sure that all cleaning
materials have been removed completely from
the floorpan.
5. Reinstall the working plates.
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10.Maintenance
10.1Inspection
PER is the value that indicates the safety state of the safety cabinet by monitoring various device parameters.
•If PER shows a value below 60, the device should be inspected.
•Independent of the PER value, the safety cabinet must be inspected on an
annual basis.
The annual inspection comprises the following checks:
•Electrical safety in accordance with national regulations.
The Perforated plate at the blanket of the sample chamber serves for the protection of the
downflow filter and prevents refluxing.
While scanning the filter surface for leak test
the perforated plate must be built-in.
•Checking the airflow conditions.
•Repeat test in accordance with NSF 49/1992, Annex F.
10.2Service
HEPA filters:
As the filter replacement is an interference with the safety system of the device,
filters must only be replaced by Kendro Laboratory Products or by adequately
trained and authorized service personnel.
NOTE – Repeat test!
Before filters are replaced the disinfection with
formaldehyde is mandatory.
After the filter replacement, a repeat test must
be performed in accordance with EN 12469 /
2000.
10.2.1UV lamps
The UV lamps should be replaced after 1500 operating hours:
1. Remove cover from lamp housing.
2. Rotate the lamp in the socket so that the lamp contacts can be removed from
the groove in the socket.
3. Insert the new lamp into the socket and rotate until the contacts engage.
1. Remove the bezel retaining screws and the bezel.
2. The luminescent tubes are retained by rotatable sockets. Rotate the tube
carefully to the removal position and remove it from the socket.
3. Insert the replacement tube and rotate it to the working position.
4. Reinstall the bezel and secure it with the screws.
10.2.3Replacing the front cover seal
Fig. 19: When the front cover seal shows visible sign of wear, the seal must be
replaced.
The sealing is slipped onto the housing frame and secured by an additional retaining strip at the lower edge of the sample chamber opening.
60
Front cover seal replacement
Fig. 19
1. Lower the front window completely and open the front cover.
2. Remove the retaining screws [3] and remove the retaining strip [2].
3. Remove the seal [1] from the housing frame retaining lip.
4. Slip the groove of the new seal onto the housing frame retaining lip and push
it on so that the seal fits evenly along the entire sealing surface.
5. Secure the retaining strip with the screws.
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10.Maintenance
10.3 Retrofitting and repairs
External communication systems, e.g. failure report systems or components for
supplying media such as gas solenoid valves can be retrofitted and integrated
into the device control.
NOTE – Retrofitting and repairs!
All retrofitting and repair work are interferences
with the safety system of the unit. Particularly modifications to the filter system and resulting changes of the airflow may impair personal
and material protection. Such work must be
carried out only by authorized service
personnel.
Discarded cabinets or unit components contain reusable materials. All components with the exception of the HEPA filters can be disposed of after having been
thoroughly cleaned and disinfected. The HEPA filters must be disposed of in accordance with the applicable national and state regulations for special solid waste.
CAUTION – Contamination hazard!
As the device can be used for processing and
treating infectious substances, it may be
contaminated.
Prior to disposal, the complete device with
filters must be decontaminated by performing
a formaldehyde sterilization!
Recyclable materials!
ComponentMaterial
Thermal insulation
components
Printed circuit boards
Plastic components, generalNote material labeling
Exterior housingSteel, painted
Device backpanelStainless steel/steel, painted
Front cover sealEMPP
Front window, side windowsMulti-layer safety glass
Operatin
foil
WorkplatesStainless steel
UV bezelsStainless steel
ArmrestsStainless steel
BatteriesAlkaline cells
Batteris (safety feature:
Lower Front W indow)
panel and indicator
Polystyrene foam, EPS/PPS compound
Enclosed electrical components coated with various
plastics, mounted on epoxy resin-bound boards.
The noise level was determined in accordance with EN ISO3744.
The sound press ure level was measured at a distance of 1 m in
front of the working opening. The measurement uncertainty is
within a range of ± 2 dB.