Heraeus Biofuge Haemo User manual

Biofuge haemo

Instructions for use

How to use this manual

Use this manual to get acquainted with your centri­fuge and its accessories.
The manual helps you to avoid inappropriate han­dling. Make sure to keep it always close to the centri­fuge.

A manual that is not kept handy cannot provide
protection against improper handling and thus
against damage to persons and objects.

The manual comprises chapters on

• Safety regulations

• Instrument description

• Rotor program and accessories

• Transportation and hook-up

• Use of the centrifuge

• Maintenance and care

• Troubleshooting

• Technical data

• Index

Overleaf you will find a graphic repre-

sentation of the control panel of the
Biofuge haemo with a survey of the

most important functions

Please fold out

12000

speed time

10

-

+

set

Biofuge

haemo

set

lid

quick

run

start
stop

INSTRUMENTS

The control panel of the
Biofuge haemo

Display

Speed

Resting state: upon pressing any key: display of

preselected speed, after 2 min switch

to standby mode (flashing red light)
During run: preselected speed
Braking: "br"
End: "0"
Lid open: "OPEN"
Resting/end: error codes (if present)

Run time ("time")

Resting/end: upon pressing any key: display of

preselected run time, after 2 min

switch to standby mode (flashing red

light)
During run: remaining run time or (with quick start)

run time passed

Keys

lid: open lid (only possible if connected

with mains supply)

quick run: short-term acceleration as long as key

is pressed, with indication of run time

passed
start/stop: normal start or manual stop
set speed/time: selection of the parameter to be

changed (speed or run time)
+/- stepwise increase/decrease of preset

values for the selected parameter

speed or run time, accelerated

change when pressed permanently

Error codes
(troubleshooting see chapter "Troubleshooting")

E-1/E-12: keys do not respond (main board defective)
E-3: no braking current: lid popped open or

manually opened during run; excess tem-

perature in the drive
br: power turned off during run or power failure
lid: lid opened during run
OPEN: lid lock mechanism out of order

Contents

For your safety........................................... 3

Proper use............................................................... 3

Improper use ........................................................... 3

Centrifuging hazardous materials ........................... 3

Handling .................................................................. 4

Conformity to current standards.............................. 5

Safety instructions in this manual ........................... 5

The Biofuge haemo ................................... 7

Safety systems........................................................ 7

Rotor chamber..................................................... 7

Lid lock mechanism............................................. 7

Properties................................................................ 8

"Quick run" operation .............................................. 8

Accessories ............................................... 9

Standard accessories ............................................. 9

Hematocrit dish and reading harp ....................... 9

Reading spiral ..................................................... 9

Hematocrit rotor................................................. 10

Fixed-angle rotor 7500 3325 for microliter tubes11

Fixed-angle rotor: technical data....................... 12

Adapters for rotor order no. 7500 3325............. 13

Other accessories ............................................. 13

Contents

Before use ................................................ 15

Where to install the centrifuge...............................15

Mains connection................................................... 15

Removing the transport protection ........................ 15

Operation.................................................. 17

Transport and installation ......................................17

Mains connection................................................... 17

Opening the lid.......................................................18

Emergency lid release .......................................18

Loading the hematocrit rotor..................................19

Inserting the hematocrit rotor.................................19

Important application information for

rotor 7500 3325 ! ..................................................21

Inserting the rotor ..................................................22

Permissible rotor temperature ...........................23

Lifetime of the rotor ............................................ 23

Removing the rotor ................................................ 24

Loading the rotor ...................................................24

Maximum loading...............................................25

Filling the tubes..................................................25

Aerosol-tight application ........................................26

Please observe the permissible filling volumes! 26

Checking for aerosol tightness ..........................27

Placing the tubes in the rotor.................................28

1

Contents

Selecting the speed............................................... 29

Selecting the run time ........................................... 30

Preselected run time.......................................... 30

Continuous operation ........................................ 30

Starting the centrifuge ........................................... 31

Changing the settings during the run .................... 32

Displaying the preset values ................................. 32

Stopping the centrifuge ......................................... 32

Stopping with preset time .................................. 32

Stopping with continuous operation .................. 34

Short-time centrifugation ....................................... 34

RCF value ............................................................. 35

Measuring the hematocrit value ............................ 36

Reading harp ..................................................... 36

Reading spiral.................................................... 36

General hints concerning hematocrit

measurements................................................... 36

Maintenance and care ............................. 38

Maintenance to be performed by the customer .... 38

Cleaning............................................................. 38

Disinfection ........................................................ 39

Decontamination................................................ 41

Autoclaving ........................................................ 42

The KENDRO service offer ................................... 43

Warranty conditions .............................................. 43

Troubleshooting.......................................44

Problems you can handle yourself ........................44

In case you must call the Service ..........................46

Technical data .......................................... 48

Component parts and performance.......................48

Electrical connections/fuses ..................................51

Speed / g diagram for rotor 7500 3325..................52

Autoclaving protocol ..............................................53

Index.......................................................... 54

2

For your safety

For your safety

Heraeus centrifuges are manufactured according to
current technical standards and regulations. Nonethe­less, centrifuges may pose dangers if

• they are not used as designed

• they are operated by untrained personnel

• their design is improperly changed

• the safety instructions are not heeded

Therefore anybody concerned with operation and
maintenance of the centrifuge must read and follow
the safety instructions.

In addition, the pertinent regulations for prevention of
accidents must be strictly followed.

This manual is an integral part of the centri­fuge assembly and must be kept close at
hand at all times.

Proper use

The centrifuge is designed to separate liquid­suspended materials having different densities and
particle size, respectively. The maximum sample den­sity is 1.2 g/cm

3

at maximum speed.

Improper use

During a run, a safety zone of 30 cm around the centri­fuge must be maintained where neither persons nor
hazardous materials may be stationed.
The centrifuge may cause harm to you or other per­sons and may damage material goods if you do not
respect the following safety measures:

Centrifuging hazardous materials

• The centrifuge is neither made inert, nor is it explo-

sion-proof. Therefore never use the centrifuge in an
explosion-prone environment.

• Explosive or flammable substances must not be

centrifuged. The same holds for substances prone
to react briskly with each other.

3

For your safety

• Do not centrifuge toxic or radioactive substances or

pathogenic microorganisms unless you have taken
proper precautions.
Such precautions can e.g. consist of biological
seals.

• Should toxins or pathogenic substances enter the

centrifuge or its parts, you must carry out the proper
procedures for disinfection (see "Maintenance and
care – Disinfection").

• Strongly corrosive substances that may cause dam-

age to materials and impair the mechanical strength
of the rotor may be centrifuged only inside protec­tive vessels.

Handling

• Never use the centrifuge unless the rotor is properly

mounted.

• Never manually open the lid if the rotor still turns.

• Use only original parts for the centrifuge. The only

exception are common glass or plastic centrifuge
tubes if these are approved for the rotor speed and
RCF values of your rotor, respectively.

• Never use the centrifuge with the lid open.

• Never use the centrifuge if the paneling has been

partially or totally removed.

• Changes in mechanical or electrical components

may be carried out only by persons authorized to
this effect by KENDRO Laboratory Products.

• You may use the centrifuge only with a properly

loaded rotor. You must not overload the rotor.

• If the rotor or the lid shows visible traces of corro-

sion or wear, you must stop using it.

• Strictly follow the rules and regulations for cleaning

and disinfection.

4

For your safety

Conformity to current standards

Heraeus centrifuges are manufactured and tested
according to the following standards and regulations:

for all voltages:

• IEC 1010-1 / EN 61010-1

• IEC 1010-2 / EN 61010-2-020

− Pollution degree 2

− Overvoltage category II

for 120 V only:

• CAN/CSA-C22.2 No. 1010.1-92

• CAN/CSA-C22.2 No. 1010.2.020-94

Safety instructions in this manual

This symbol denotes potential hazards to
persons.

This symbol denotes potential damage to the
centrifuge or parts in its immediate surround­ings.

General hints are marked with this symbol.

In addition, you are asked to adhere to the pertinent
regulations, in Germany

• Regulations for prevention of accidents VBG 4

• Regulations for prevention of accidents VBG 5

• Regulations for prevention of accidents VBG 7z

5

For your safety

for your notes

6

The Biofuge haemo

The Biofuge haemo

The figure below shows the Biofuge haemo with the lid
opened. In this state, the standard display at the end of
a run and after opening the lid is "OPEN". After a cou­ple of minutes the display changes to the standby
mode, and all display panels turn dark.

ACHTUNG

Rotor vorschriftsmäßig
auf Welle befestigen

ATTENTION

Fixer le rotor sur
l'arbre conformément
aux préscriptions

ATTENTION

Rotor must be fitted in
accordance with the
instruction manual

ATENCIÓN

Fijar el rotor en el
arbol según las
prescripciones

Safety systems

The Biofuge haemo is equipped with a number of
safety systems.

Rotor chamber

The rotor chamber is integrated into the steel case.
When the lid is closed, the rotor chamber is sealed
against the surroundings by a rubber ring with a spe­cial profile that allows cooling air to pass through the
rotor chamber.

Warning if lid is manually opened during a run, or if
lid lock mechanism is out of order

If the lid is manually opened during a run, or if the lid
lock mechanism is out of order, a corresponding mes­sage appears in the display ("Lid" and "OPEN",
respectively).

Lid lock mechanism

You can open the lid only when the power is turned on
and the rotor has practically come to a halt (< 80 rpm).
You can start the centrifuge only if the lid is properly
closed.

Emergency lid release

In order to permit you to remove samples even after a
power failure, the centrifuge is equipped with an emer­gency lid release (see „Operation“).

7

The Biofuge haemo

Properties

The Biofuge haemo is an air-cooled benchtop centri­fuge for the determination of the hematocrit.
It can also be used for the preparation of sensitive
samples in the biochemical and medical laboratory.
The rotor accepts common centrifuge tubes up to a
volume of 2 ml.
The preset speed is reached in seconds. You can also
spin samples for only a few seconds using the "quick
run" key if this is required for the task in question. The
long-lived, maintenance-free induction motor provides
quiet and vibration-free operation even at high speeds.
The user-friendly control panel permits easy operation.
At the start the preset speed and run time are dis­played. During a run, the remaining run time is dis­played: above 1 min in minutes and below 1 min in
seconds.
If you press the "+" or "-" key repeatedly, you increase
the corresponding preset parameter (speed or run
time) stepwise. If you press and hold down the chosen
key, the respective value changes continuously, at first
slowly and after a few seconds at an accelerated pace.

Air cooling of the Biofuge haemo

During a run, the spinning rotor creates frictional heat.
This leads to a temperature increase of the rotor, the
tubes and finally of the samples. The extent of warming
depends on:

• run time

• temperature of the environment

• location of the centrifuge

• rotor speed

The Biofuge haemo is equipped with air cooling to
prevent overheating of the samples. The cooling is
particularly effective in the cold room (lowest permissi­ble temperature +4 °C).

"Quick run" operation

If you press and hold the "quick run" key, the rotor is
accelerated with maximum force up to the maximum
speed.

8

Accessories

1

3

4

5

6

7

8

9

1

0

1

1

1

2

1

3

1

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1

5

1

6

1

7

1

8

1

9

2

0

2

1

2

2

2

3

2

4

H

E

R

A

E

U

S

I

N

S

T

R

U

M

E

N

T

S

C

a

t

-

N

o

1

0

6

7

I

I

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2

0

0

0

/

R

P

M

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7

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8

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9

2

0

2

1

2

2

2

3

2

4

Accessories

Standard accessories

Hematocrit dish and reading harp

The Biofuge haemo is delivered with a special rotor
(hematocrit dish or rotor) equipped with 24 guide not­ches for receiving blood capillaries.
A reading harp for determining hematocrit values after
removing the capillaries is delivered with the instru­ment.

Reading spiral

As an option, you can order a reading spiral which is
placed directly onto the rotor for reading hematocrit
values in situ.

9

Accessories

Hematocrit rotor

Table 1: Hematocrit rotor order. no. 7500 1067 for biofuge haemo

number of samples 24 blood capillaries

temperature range -4 °C to +40 °C

maximum capacity 24 capillaries with 1.2 to 1.8 mm diameter and 75 + mm length

maximum speed n

maximum RCF value at n

10

12000

max

14926

max

Accessories

Additional accessories

Fixed-angle rotor 7500 3325 for microliter tubes

Optionally you can order for the Biofuge haemo a
fixed-angle rotor with 24 holes for placing microliter
tubes with a volume of 1.5 or 2.0 ml.
In addition, three sets of adapters with 24 reduction
sleeves each are available on request. With the adapt­ers you can centrifuge all commercially available mi­croliter tubes with volumes of 0.2 ml to 0.6 ml as well
as 0.2 ml PCR reaction vessels.
Please refer to our sales documents for detailed infor­mation on accessories including technical data and
order numbers.

3

1

1

2

4

1

1

1

5

1

6

a

1

d

7

2

4

x

1

4

8

g

1

9

p

p

2

1

0

/

9

6

2

#

1

3

3

2

2

2

2

3

1

S

m

T

a

N

0

x

E

1

M

l

o

U

R

T

9

S

N

I

S

8

U

E

A

7

R

E

H

6

m

p

r

5

0

0

4

0

3

1

4

x

a

m

3

2

4

2

1

11

Accessories

Fixed-angle rotor: technical data

Table 1: Fixed-angle rotor order no. 7500 3325 for Biofuge haemo

no. of places/volume 24 x 1.5 / 2 ml

maximum permissible load 24 x 4 g

temperature range -4 °C to +40 °C

maximum speed n

maximum RCF value at n

minimum speed n

minimum RCF value at n

[min-1] 13000

max

16060

max

[min-1] 2000

min

380

min

maximum radius [cm] 8.5

minimum radius [cm] 5.9

angle of incidence 40°

maximum kinetic energy [s] 1.65 kNm

12

Adapters for rotor order no. 7500 3325

Table 2: Adapters for the fixed-angle rotor of the Biofuge haemo

Adapter Dimensions

(∅ x H)

reduction sleeve PCR 6,2 x 20 mm 0.2 ml 24 gray 7600 3750

reduction sleeve 8 x 43.5 mm 0.5 / 0.6 ml 24 turquoise 7600 3758

reduction sleeve 6 x 46 mm 0.25 / 0.4 ml 24 red 7600 3759

Other accessories

Capacity Number per

Set

Color Order No.

• Reading harp 7600 0938

• Reading spiral 7500 1236

For use with the fixed-angle rotor also available:

• fixed-angle rotor 24 x 1.5 / 2 ml 7500 3325

• special cap nut for fixing the rotor

• 10 mm tubular hexagon wrench for tightening the

cap nut

cap nut
order no.
70056208

tubular socket
wrench
order no.

2036 0072
The printed documents consist of the delivery notes
and this Manual.

Accessories

13

Accessories

for your notes

14

Before use

Before use

Where to install the centrifuge

The centrifuge must be operated in a place meeting the
following criteria:

• A safety zone of 30 cm around the centrifuge must

be maintained. Hazardous materials must not be
kept within this zone during centrifugation.

• The substructure must be stable and resonance-

free. A good support is provided by a plane labora­tory bench or a large laboratory carriage with cast­ers that may be locked.

• To ensure sufficient air circulation, a minimum dis-

tance from the wall of 10 cm at the back and of
15 cm on each side must be kept.

• The centrifuge must be protected from heat and

direct sunshine.

• The location should be well ventilated.

Mains connection

Make sure that your mains voltage and frequency
match the specifications on the instrument.

Removing the transport protection

Turn the instrument on. The display panel shows for
about 6 s the routine internal software check sequence.
Open the lid by pressing the "open lid" key and remove
the transport protection for the rotor.
Check that the rotor moves freely by lightly turning it,
and make sure the rotor is tightly screwed on.

15

Before use

for your notes

16

Operation

Operation

Transport and installation

Damage to the centrifuge by jolting!

Transport the centrifuge only in the
upright position using the special box
provided with the instrument and
secure it properly. Place the centri­fuge carefully.

Make sure the rotor transport protection
has been removed before starting the
instrument!

The Biofuge haemo is now ready for use.

Mains connection

Make sure that your mains voltage and frequency
match the specifications on the instrument before con­necting the centrifuge to the mains supply.

The instrument does not have a mains switch.

After you have established the mains connection, the
following reading appears in the control panel:

This tells you that the

lid

+

speed time

set

8.8.

set

-

internal check of its soft-

start
stop

ware.

INSTRUMENTS

instrument carries out an

quick

run

8.8.8.8.8.

Biofuge

haemo

If the lid is closed, the display in the panels "speed" and
"time" changes to "0" after a couple of seconds:

With opened lid, the
"speed" panel displays

0

the message "OPEN"
instead.

Biofuge

0

speed time

set

haemo

set

If you do not intervene, the display changes to the
standby mode after 2 minutes. The displays turn dark;
however, the centrifuge is ready for use at any time.
This is signaled by the flashing red light in the "time"
panel.

17

Operation

Opening the lid

Press the "lid" key. The lid lock mechanism is released,
and the lid pops open.

Emergency lid release

In case of a power failure you cannot open the lid nor­mally using the "lid" key (see previous section). To
permit unloading even in this case, the centrifuge is
equipped with a mechanical lid unlocking system. How­ever, you may use this system only in case of emer­gency.

Rotor can spin at high speed! Touch­ing it may cause severe injuries!

Always wait for several minutes until
the rotor has come to a complete
stop. Without power the brake does
not function, and braking takes much
longer than normal!

Should it be necessary to open the lid manually, carry
out the following steps:

1. Make sure the rotor stands still (consult window in
the lid).

2. Unplug the mains plug.

3. Push a thin screwdriver or another suitable tool

horizontally from each
side through the two
openings in the side
panels of the centri­fuge (see figure).
Push the locking pins
under the side panels
simultaneously from
both sides until the lid
unlocks audibly. Re-

move the auxiliary tools and open the lid.

4. If the rotor still turns, close lid immediately and wait
until it has come to a complete stop

Never brake the rotor using your hands
or tools!

5. As soon as the rotor stands still, remove your

samples and close the lid.

18

Operation

Loading the hematocrit rotor

After filling and sealing the capillaries, place them into
the guide notches with the sealed ends pointing out­wards.

The centrifuge must always be loaded
weight-symmetrically to avoid unbal­ance, unsteady runs and subsequent
damage.

Inserting the hematocrit rotor

After checking for correct loading, you may insert the
rotor into the centrifuge.

Possible damage to drive and rotor!
Do not push down the rotor by force.
If the rotor is tilted, carefully remove

To put the rotor into position, proceed as follows:

1. Open the lid and make sure that the rotor chamber
is clean. Remove eventual dust, foreign material or
sample residues.

and reinsert it.

2. Turn the rotor so that the driving pin of the motor
shaft fits into the groove on the underside of the ro­tor, and slide the rotor onto the drive shaft until the
rotor hub rests on the shaft (cf. Figure).

Befestigungsmutter

4

4

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3

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1

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R

9

9

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U

M

M

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2

E

E

2

2

0

0

N

N

0

0

2

2

1

1

2

2

2

2

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M

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H

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A

E

E

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U

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N

S

S

T

R

T

T

S

S

C

C

a

a

t

t

-

-

N

N

o

o

2

2

1

1

0

0

6

6

2

2

3

3

2

2

4

4

2

2

4

4

0

0

P

P

1

1

R

R

1

1

/

/

0

0

1

1

0

0

9

9

9

9

0

0

8

8

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0

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7

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1

1

19

Operation

3. Tighten the fastening nut clockwise using a coin.

4. Screw the rotor cap on with the plastic knob.

You must fasten the rotor cap before
starting the centrifuge.

Starting and stopping the centrifuge is described start­ing on page 31, evaluation of the haematocrit on
page 36.

Removing the hematocrit rotor
For removing the hematocrit rotor you must carry out
the steps described above under "Inserting the hema­tocrit rotor" in reverse order.

Irreparable damage to the motor!
Never tilt the rotor during removal.

1. Open the centrifuge lid.

2. Unscrew and remove the rotor cap.

3. Loosen the rotor fastening nut counterclockwise
using a coin. Grab the rotor centrally at the hub and
pull out perpendicularly. Do not tilt.

The following description applies only to the fixed-angle
rotor 7500 3325. If you use the centrifuge only for he­matocrit measurements, you may skip this section and
continue on page 29 ("Selecting the speed").

20

Operation

Important application information for rotor
7500 3325 !

To attach the rotor, use the acorn nut with ball head!
(Order no. 70056208)

To tighten the acorn nut, use the 10 mm tubular hexa­gon box spanner. (Order no. 20360072)

Please always close your microlitre containers care­fully. Open container lids can damage the rotor lid.
For some special applications the container lids must
remain unsealed. If this is the case, please use the
screw top (order no. 75003326) instead of the standard
snap-on lid (order no. 70901111).

A) Position the snap-on lid.

Press the rotor lid down onto the rotor until
the snap-on catch engages onto the ball
of the acorn nut.

B) Remove the snap-on lid.

By activating the grip cap, the lock is re­leased and the rotor lid can be removed.

21

Operation

Inserting the rotor

Possible damage to drive and rotor!

You may insert the rotor only if the
temperature of the drive, the rotor and
the cap nut is between 10 °C and

30 °C.

The rotor approved for the Biofuge haemo is shown in
the chapter "Accessories". Check whether the rotor you
want to insert corresponds to the figure in this chapter.
To insert the rotor, you need the rotor, the cap nut and
the tubular socket wrench supplied (see chapter "Ac­cessories – pieces delivered").

Possible damage to drive and rotor!

Do not push down the rotor by force. If
you cannot screw on the cap nut eas­ily, remove the rotor and reinsert it.

To put the rotor into position, proceed as follows

1. Open the lid and make sure that the rotor chamber
is clean. Remove eventual dust, foreign material or
sample residues. The thread and the O-ring on the
motor shaft must be in perfect condition.

2. Turn the rotor so that the notch for engaging the
drive shaft points downward.

3. Place the rotor on top of the drive shaft so that the
notch of the rotor is located precisely above the re­taining pin.

4. Push the rotor gently down until the thread is com­pletely laid bare (see figure).

22

5. If you have placed the rotor correctly, you can screw
on the cap nut easily and secure it with the tubular
socket wrench delivered with the instrument.

6. Snap the rotor cap onto the rotor (the optional her­metic lid 7500 3326 is screwed on centrally with the
lid nut).

Regularly check the proper positioning of
the rotor and re-tighten the cap nut as

needed.

Operation

Permissible rotor temperature

The rotor 7500 3325 may be used only
within a temperature range of –4 °C to
+40 °C. Pre-cooling in the freezer is not
permitted.

Lifetime of the rotor

There are no restrictions to the service life of the high
performance rotor 7500 3325 B. However please ob­serve the following due to safety reasons:

Rotors and accessories made of plas­tic should not be exposed to direct
sunlight and UV rays!

If the rotor shows signs of discolor­ation, deformation or wear, or is out of
balance it must be exchanged straight
away!

23

Operation

Removing the rotor

To remove the rotor, you must follow the steps de­scribed above in reverse order.
With the hermetic lid, you may in case of contamination
separate the rotor from the drive without opening the
lid! In this case you can open rotor upon removal from
the centrifuge using e.g. a safety work bench before
decontaminating it.

Danger of irreparable motor damage!

Never tilt the rotor. Always grab it in
the middle and pull out perpendicu­larly.

1. Open the lid of the centrifuge.

2. Remove rotor cap (not necessary with the hermetic
lid).

3. Screw the cap nut open by turning it counterclock­wise using the socket wrench delivered with the in­strument. Remove the cap nut.

4. Grab the rotor in the middle and pull gently upwards
off the drive shaft. Be careful not to jam it.

Loading the rotor

As an accessory, you can optionally order the fixed­angle rotor 7500 3325 with 24 places. Three sets of
adapters and a hermetic lid are available for this rotor
(see under "Accessories").

Improper or improperly combined ac­cessories may cause severe damage
to the centrifuge!

Use only the parts and accessories
listed in the chapter "Accessories".

24

Operation

Maximum loading

Overloading may cause the rotor to
explode! Exploding parts may severely
damage the centrifuge!

Never exceed the maximum permissi­ble load of 4 g per place.

The Biofuge haemo can reach high rotational speeds
implying enormous centrifugal force. The rotor is de­signed in a way warranting sufficient residual strength
even at the highest permissible speed.
However, this safety system presupposes that the
maximum permissible load of the rotor is not exceeded.
The maximum load for the rotor has been calculated for
a sample density of 1,2 g . cm

-3

. If the density of your
sample is higher, you must either reduce its volume (to
an overall weight of 4 g) or calculate a reduced speed
n

according to the following formula:

perm

−

∗

n

perm

n

= ∗

max

weight of tube with sample

weight of tube with denser sample

gcm

123,

Filling the tubes

Separations by centrifugation function best when the
unbalance of the rotor is minimized because separated
zones are not perturbed by vibration. It is therefore
important to balance the centrifuge tubes as well as
possible.
To minimize unbalance you should fill the tubes as
evenly as possible. You can achieve this by eye. How­ever, you must nonetheless ensure that opposite tubes
are filled to the same level.

25

Operation

Aerosol-tight application

only with screw-on top 75003326 and
not with open container lids!

The following steps have to be carried out:

• Lubricate the seals before inserting them (lubricant

order no. 75003500)

• Insert the seal (C profile) in the groove at the side

of the body of the rotor.

• Insert the O-ring into the inner groove on the

screw-on top.

Attention :

Please check that your sample containers are suitable
for the centrifugal application desired.

(16060 x g ; temperature in uncooled devices approx.
10 K above room temperature)

Please observe the permissible filling volumes!

Nominal volume: Permissible volume:

2.0 ml - 1.5 ml

1.5 ml - 1.0 ml
others -

2

/3 nominal volume

The sealing elements are to be checked regularly for
damage to the shape and surface!
Exchange faulty parts immediately (spare sealing rings

75003268)

The snap-on lid is not suitable for
aerosol-tight application!

26

Operation

Checking for aerosol tightness

Check the aerosol tightness of your
rotor whenever appropriate.

To carry out the test, proceed as follows:

• Carefully clean and degrease the rotor chamber

wall, then attach an adhesive white paper strip
(about 4 x 2 cm) so that liquid leaking out of the ro­tor may precipitate on it.

• Fill all places of the respective rotor with water

according to the following Table. Insert the rotor
into the centrifuge and fasten it.

• Carefully place the amount of test liquid (0.5 %

sodium fluorescein in water) specified in the col­umn “leakage test” into the lower part of the rotor
within a virtual circle comprising the vessel bores
(not the bores themselves) using a pipette or sy­ringe.

• Place the rotor lid on top and screw it on.

ATTENTION: Make sure that there is no spilled
test liquid on the rotor (clean if necessary)!

• Carry out a test run for 10 minutes at maximum

rotor speed and 23 °C ambient temperature.

• Check the paper strip under UV light (preferentially

in a darkened room):
If there is no detectable fluorescence, the test is
considered passed.

• Finally rinse rotor, rotor lid and lid seal in running

water and allow to dry.

27

Operation

Placing the tubes in the rotor

The rotor must be loaded symmetrically. When loading
the rotor only partially, you must ensure that opposite
bores always receive tubes of equal weight (when cen­trifuging a single sample, place a centrifuge tube e.g.
filled with water). The following figure gives examples
for proper loading.

Improper loading can in the worst case
lead to damage to rotor and centrifuge.
Unbalance not only causes a noisy
run, but rapidly damages the motor
suspension.

improperly loaded rotors

After loading the rotor, snap on the rotor cap until it
locks into place. The optional hermetic lid 75003326 is
screwed onto the rotor with the central nut. Close the
lid of the centrifuge by firmly pressing it down. There
must be a clicking sound, and the lid must be locked so
that it cannot be opened manually.

28

properly loaded rotors

Operation

Selecting the speed

For hematocrit measurements the
speed is limited to maximally 12 000
rpm!

The minimum speed of the drive is 1,600 rpm, the
maximum speed 13,000 rpm. The built-in microproces­sor prevents higher or lower speed settings. Between
these extremes, you can select the speed in steps of
100 rpm using the following procedure:

1. Press the "set" key in the "speed" panel (press twice

if the centrifuge is in the standby mode). The speed
last entered appears in the display. The third digit
from the right (in the example the figure "5") flashes,
thus indicating that you can change the setting in
multiples of 100 rpm.

2. Press one of the "set" keys "+" (for an increase) or

"-" (for a decrease). By pressing the selected key
briefly, you increase or decrease the speed in steps
of 100 rpm. This option is supposed to be used for
small changes and fine tuning.

3. If you keep the key pressed, the display changes at

first slowly and after a few seconds at an acceler­ated pace.

6500

speed time

set

4. Release the key as soon as you are close to the
desired value, and fine tune if necessary by repeat­edly pressing the selected key (or its counterpart if
you have proceeded too far in one direction). The
third digit from the right keeps flashing. The speed is
stored by again pressing one of the "set" keys or
"start".

0

set

29

Operation

Selecting the run time

You can select a run time between 1 and 99 min or
continuous operation.

For hematocrit determinations you
should select a run time of 10 min for a
standard blood column of about
65 mm.

The evaluation of hematocrit measurements is detailed
on page 35.

Preselected run time

To predetermine the run time, proceed as follows:

1. Press the "set" key in the "time" panel (press twice if
the centrifuge is in the standby mode). The run time
last entered appears in the display. The right-hand
digit (in the example on the following page the figure
"5") flashes, thus indicating that you can change the
setting in minutes.

2. Press one of the "set" keys "+" (increase) or "-" (de­crease). By pressing the selected key briefly, you
increase or decrease the preset run time in steps of
1 min. This option is supposed to be used for small
changes and fine tuning.

3. If you keep the key pressed, the display changes at
first slowly and after a few seconds at an acceler­ated pace.

0

speed time

set

15

set

4. Release the key as soon as you are close to the
desired value and fine tune if necessary by repeat­edly pressing the selected key (or its counterpart if
you have proceeded too far). The display keeps
flashing. The run time is stored by again pressing
one of the "set" keys or "start".

Continuous operation

For continuous operation, press the "set" key in the
"time" panel and then the "-" key until "hd" (for "hold")
appears in the display.
With this setting, the centrifuge keeps running until
stopped manually.
Please note that the lifetime of centrifuge tubes, par­ticularly plastic ones, is limited, and that they may be
damaged by continuous operation!

30

Operation

Starting the centrifuge

Once the rotor is in place and the lid closed, you can
start the centrifuge.

lid

quick

run

start
stop

Press the "start/stop" key in the control panel. The dis­play panel shows the preselected values for speed and
run time (see Figure below). The centrifuge accelerates
to the preselected value. Simultaneously, the run time
display starts going backward from the preset time,
giving the remaining run time in minutes. After reaching
the last minute the display switches to seconds remain­ing.

If you have chosen continuous operation, the time dis­play goes forward instead of backward.

This is signalled by the lit green LEDs
to the right of the keys "quick run" and
"start/stop" (see Figure). If you are not
sure which values you have prese­lected, you can determine them by
pressing one of the "set" keys. The
function not currently selected displays
"0" irrespective of the actual setting.

After startup, alle LEDs except the red one for "stop"
turn dark. This is now the only function available.

31

Operation

12000

Biofuge

haemo

speed time

set

10

set

Changing the settings during the run

You can increase the speed setting for the current run
by pressing "set" in the "speed" panel, adjusting the
value with the "+" key and again pressing "set". If you
use the same procedure to lower the speed (by press­ing "-" instead of "+"), the centrifuge brakes to standstill
and then accelerates again to the newly selected
speed.
Likewise you can alter the preset run time during a run
by pressing consecutively "set" in the "time" panel, "+"
or "-" and again "set".

Displaying the preset values

You can check the preset values for speed and run
time by pressing one of the "set" keys. If you press the
key again, the display turns dark.

lid

quick

run

start
stop

INSTRUMENTS

Biofuge

0

speed time

set

haemo

0

set

+

-

Stopping the centrifuge

Stopping with preset time

Normally the run time has been preselected, and all
you have to do is wait until the centrifuge terminates
the run automatically. During braking, the "speed"
panel reads "br" and the "time" panel "0":

32

Operation

speed time

0

set

Biofuge

haemo

set

+

-

lid

quick

run

start
stop

INSTRUMENTS

Shortly before the rotor stops, a two-step acoustic sig­nal alerts you to the fact.

As soon as the rotor stands still, both

lid

panels display the message "0", and
the LED next to the "lid" key lights up.

By pressing the "lid" key, you can now

quick

run

open the lid and remove your samples.
The "speed" panel displays "OPEN".
After 2 minutes the instrument switches

start
stop

to the standby mode, and all displays
turn dark. You can even now open the
lid by pressing the "lid" key.
You can stop the centrifuge at any time
by pressing the "stop" key.

33

Operation

Stopping with continuous operation

If you have chosen continuous operation, you must
stop the centrifuge manually by pressing the "stop" key.
The centrifuge starts braking at once and stops within a
few seconds. The displays and options are the same
as described in the previous section.

Short-time centrifugation

For short-term operation, the Biofuge haemo is equip­ped with a "quick run" function.
This option is available whenever the LED next to the
"quick run" key is lit.

The preset speed is ignored! Centri­fuge tubes and rotor may be dam­aged!

Check carefully whether you have to
maintain a specific speed.

Never use this option for hematocrit
measurements!

When you press and hold the "quick run" key, the cen­trifuge accelerates with full power up to the maximum
speed of 13,000 rpm unless you release the key. The
preset speed is ignored.
During acceleration the time is counted forward in sec­onds. After 60 seconds the display changes to the min­ute mode.

The "quick run" mode is not recommended for sensitive
samples with fluffy sediment.

34

Operation

RCF value

The relative centrifugal force (RCF) is usually given in
multiples of the earth gravity g. It is a dimensionless
number that allows one to compare the efficiency of
separation or sedimentation of diverse instruments,
since it is independent of the instrument used. The only
values entered in the equation are radius and speed of
centrifugation:

RCF

.

r = radius of centrifugation in cm
n = speed in rpm

2

n





r=∗

1000

∗1118







The centrifuge reaches an RCF value
of 16,060 at the maximum speed of
13,000 min

-1

! Check carefully
whether your tubes are designed for
such a strain, and reduce the speed
if necessary.

The maximum RCF value refers to the maximum radius
of the tube.
For the fixed-angle rotor 7500 3325 the above formula
yields for the bottom of the bore (radius 8.5 cm) a
maximum RCF value of 16,060:

13 000

RZB =∗



.




1000

2

,



∗=1118

8 5 16 060

.,




Please note that this value becomes lower
depending on the tubes and adapters used.

You may take this into account when calculating the
RCF value for your application.
The figure on the last page of this manual gives a
graphic representation of the relation between speed
and RCF.
Apart from the maximum RCF value RCF

(lower

max

line) this graph also shows the minimum RCF value
RCF

, calculated for the meniscus of the sample (up-

min

per line).

35

Operation

Measuring the hematocrit value

The hematocrit value is determined after removal of the
rotor cap (see page 20). There are two evaluation me­thods: reading harp and reading spiral.

Reading harp

The reading harp is the standard accessory delivered
with the centrifuge. To read the hematocrit values, you
must remove the capillaries one by one from the hema­tocrit dish.
Place the capillary with the lower end of the blood col­umn on the zero line and the upper end on the 100 %
line of the reading harp. At the boundary between the
erythrocytes and the plasma you can now read the
percentage of packed cells. Be careful to place the the
capillary at right angles to the zero line.

Reading spiral

The reading spiral is available as additional accessory.
To measure hematocrit values, remove the rotor cap
(see page 20) and place the spiral on the hematocrit
dish, which may remain in the centrifuge. Using the
outer knurled (eccentric) screw, turn the zero line so
that it comes to lie on the outer end of the blood col­umn; then place the 100 % line of the reading spiral
over the meniscus using the knurled screw in the mid­dle.

You can now read the hematocrit value directly from
above on the graduation. Once you are finished, you
can dispose of all capillaries at once. Loosen the rotor
fastening screw with a coin by turning it counterclock­wise, lift the hematocrit dish vertically out of the centri­fuge and eject the capillaries into the waste disposal by
overturning the dish.

General hints concerning hematocrit
measurements

A certain amount of standardization is necessary to
obtain reproducible results, both in the interest of the
patients and blood donors and for the sake of compa­rability of sciencific data.
The German standard DIN 58933 describes a practical
standard. The procedure outlined in this manual has
been designed in accordance with this standard. We
recommend all users to obtain a copy of it.

36

for your notes

Operation

37

Maintenance and care

Maintenance and care

Maintenance to be performed by the cus­tomer

For the protection of persons, environment and mate­rial you are obliged to clean the centrifuge regularly
and to disinfect it if necessary.

Unsuitable cleaning agents or disinfec­tion procedures may damage the cen­trifuge and its accessories!

If you intend to use cleaning agents
or disinfection procedures not rec­ommended by the manufacturer, you
have to make sure by consulting the
manufacturer, that the procedure
foreseen does not cause any dam­ages to the instrument!

Cleaning

Pull mains plug before cleaning the
instrument!

Clean the casing, the rotor chamber, the rotor and the
accessories regularly and in case of need. This is indi­cated both for reasons of hygiene and to prevent corro­sion due to contamination sticking to the instrument
and its accessories.
Clean them with mild agents of pH values ranging from
6 to 8.

For other cleaning agents please consult KENDRO
Services!

Immediately after cleaning, dry the aluminum parts or
put them into a warm-air dryer at a temperature not
exceeding 50°C.

38

Maintenance and care

During cleaning liquids and espe­cially organic solvents should not
come into contact with the drive
shaft and the ball bearing.

Organic solvents may decompose
the lubricant of the motor bearing.
The drive shaft may block.

Disinfection

If a centrifuge tube containing infectious material leaks
during a run, you have to disinfect the centrifuge im­mediately.

Infectious material could enter the centri­fuge if spills or tube breakage occur

Danger of infection may occur upon
contact
ures for personnel!

Mind the permissible filling volumes and
loading limits for the tubes

In case of contamination the operator has
to make sure, that no further persons are
jeopardized

Contaminated parts have to be decontami­nated immediately

If required further protective measures
have to be initiated

! Take appropriate protective meas-

!

!

.

.

.

39

Maintenance and care

Rotor and rotor chamber must be treated with a neu­tral, universal disinfectant. Best suited for this purpose
are disinfectant sprays, ensuring that all rotor and
accessory surfaces are covered evenly.

• Please use 70% ethanol for disinfection.

Please note the safety measures and
handling hints when applying these
substances!

For other disinfectants please consult KENDRO
Services!

• You may disinfect the rotor and the accessories as

described in the following section. Be sure to follow
the pertinent safety procedures for handling infec­tious material.

1. Pull mains plug.

2. Unscrew the rotor chuck.

3. Grab the rotor with both hands and pull it perpen­dicularly off the drive shaft.

4. Remove the centrifuge tubes and adapters, and
disinfect them or dispose of them as necessary.

5. Treat the rotor and the rotor lid according to the
instructions given for the disinfectant (soaking in liq­uid or spraying). You must strictly observe the
specified action times!

6. Turn the rotor head down and drain off the disinfec­tant. Thereafter thoroughly rinse rotor and lid with
water.

7. Dispose of the disinfectant according to valid regula­tions.

8. Aluminum rotors have to be treated with anticorro­sive protective oil subsequently.

40

Maintenance and care

Disinfection with bleaching lye

These agents contain highly aggres­sive hypochlorites and must not be
used with aluminum rotors!

To protect the rotor 75003325 as far as possible you
must take the following precautions:

1. Avoid high temperatures!
The bleaching solution and the rotor should not be
warmer than ca. 25 °C.

2. Do not let the bleaching solution act longer than
absolutely necessary!

3. After disinfection, rinse the rotor thoroughly with
distilled water and allow to dry.

Decontamination

For general radioactive decontamination, use a solution
of equal parts of 70% ethanol, 10% SDS and water.
Follow this with ethanol rinses, then de-ionized water
rinses, and dry with a soft absorbent cloth.

Dispose of all washing solutions in appropri­ate radioactive waste containers!

41

Maintenance and care

Autoclaving

Check whether autoclaving is permitted!

You may autoclave the rotor and the adapters at
121 °C.
Maximum permissible autoclaving cycle:
20 min at 121 °C.

For reasons of safety you may autoclave
the rotor 7500 3325 maximally 10 times!

The rotor must be cleaned and rinsed with distilled
water before being autoclaved. Remove the rotor lid,
the centrifuge tubes and the adapters. Place plastic
rotors on an even surface to avoid deformation.

Chemical additives to the steam are not
permitted.

Never exceed the maximum permis­sible values for autoclaving tempera­ture and autoclaving time.

Should the rotor show signs of wear,
you must stop using it!

42

Maintenance and care

The KENDRO service offer

Kendro Laboratory Products recommends annual ser­vicing of the centrifuge and the accessories by author­ized customer service or trained professionals. The
customer service personnel is inspecting:

• the electrical installations

• the suitability of the location

• the lid lock mechanism and the safety circuit

• the rotor

• the rotor fastening and the drive shaft

Defective material is exchanged.
KENDRO offers inspection and service contracts
covering it. Inspection costs are charged as flat-rate
contracts.

Necessary repairs are carried out free of cost within the
warranty conditions, and against payment after expira­tion of the warranty period.

Warranty conditions

The warranty period starts with the day of delivery.
Within the warranty period the centrifuge is repaired or
replaced free of cost if there are provable faults in ma­terials or workmanship.
Conditions for a warranty are:

• the centrifuge is used according to the instructions

of use

• mounting, extensions, settings, alterations or repairs

are carried out exclusively by personnel authorized
by KENDRO

• the required maintenance and care procedures are

carried out regularly.

43

Troubleshooting

Troubleshooting

Problems you can handle yourself

If problems other than those described in the following tables arise, you must consult your nearest au-

Error Behavior of the centrifuge Possible cause(s) and measures to be taken

thorized service.

Displays remain dark The motor stops.

The rotor stops without
braking.
The lid cannot be opened.

Displays fail briefly The motor stops suddenly.

The rotor stops without
braking.
The display reads "br", see br.

Lid cannot be opened

Pressing the "lid" key has no
effect.

44

Mains failure or not connected

1. Check the mains connection.

2. If the mains connection is OK, call the nearest Service.

Brief interruption of mains supply

1. Check whether the plug is plugged in properly.

2. Restart the centrifuge.

Lid not correctly engaged or lid warped.

Press lid down in the middle of the front section.

Heat monitoring relays in the lid unlocking magnets have
been actuated

(repeated pressing of the "lid" key within a few seconds).
Press key again after a short pause (approx. 1 min).

Error Behavior of the centrifuge Possible cause(s) and measures to be taken

Troubleshooting

Centrifuge is exceptionally

noisy.

br Mains connection interrupted

during run

E-1 or E-12

Rotor does not start Pull mains plug and plug in again.

E-3 No braking current; drive out

of order, rotor stops without
braking

1. Stop the centrifuge by pressing the "stop" key, in case of
emergency pull mains plug.

2. Wait until the centrifuge stands still.

3. Check whether the rotor is properly loaded.

4. Check whether a broken vessel, damage to the rotor or
motor malfunction was responsible for the noise.

5. If you cannot locate and solve the problem, call Service.

If the plug was pulled inadvertently, plug in again.
Wait for about 75 seconds. The centrifuge comes to a stop
without braking.

If the error persists, call Service.

Lid opened during run.

Wait until the rotor has come to a complete stop (check
window in the lid). Pull mains plug and plug in again. If error
persists, call nearest Service.

Motor overheated.

Room temperature too high or insufficient air cooling. Pull
mains plug and wait 30 min. Check all vents and clean with
small brush.

45

Troubleshooting

Error Behavior of the centrifuge Possible cause(s) and measures to be taken

"Lid" appears in the
display

Display "OPEN“
appears although lid is
closed

Flashing red point in
"time" panel

Motor stops.
Rotor stops without braking.

Start impossible

Displays remain dark
Not an error; instrument is on
standby

In case you must call the Service

Should you require our Service, please tell us the order
no. and serial number of the instrument. You find the
pertinent information at the back of the instrument near
the socket for the mains plug.

Lid lock mechanism out of order

1. Press the lid shut. The centrifuge stops without braking.

2. If error persists, call nearest Service.

Control lid lock mechanism. The lock must be engaged on
both sides.
If error persists, call nearest Service.

Press any key. With "start/stop" or "quick run", the centrifuge
starts running at once. With the "lid" key, the lid pops open,
and the display reads "OPEN".

46

Troubleshooting

47

Technical data

Technical data

Component parts and performance

Part / function Description

Design

Armored case: steel
Frame: torsion resistant, hot galvanized 4-mm steel plates
Body and front panel: impact-resistant, highly dampening plastic
Lid: stove-enameled steel plate

Keys and display panel Keys and display panel covered with easy care protective foil

Rotor chamber Material: impact resistant plastic

Dimensions (diameter x height): 222 mm x 110 mm

Lid release Electromagnetic release with "lid" key when connected to mains

Lid lock Automatic locking when the lid is pressed shut

Emergency lid release Lid release in case of power failure: emergency opening with auxiliary tool.

48

Technical data

Part / function Description

Start "start/stop"-key

Stop "start/stop"-key

Short-term start and stop, respectively „Quick run“-key : short-term run when pressed permanently; stop when

released

Indication of operating state Preset values for speed and run time ar displayed during the run.

End of centrifugation The panels "speed" and "time" read "0".

parameter memory,
parameter display

Speed selection adjustable in steps of 100 min-1

Run time selection adjustable in minutes from 1 min to 99 min;

Time display in „quick run“ mode between 1 s and 60 s in seconds, over 1 min in minutes

diagnostics

• speed

• run time

in the range of 1600 min

„hd“-mode: continuous operation

• lid not properly closed: display "OPEN";

• indication of general malfunctions (ERROR codes)

-1

to 13000 min-1

49

Technical data

Parameter Value/description

environmental conditions - indoor use

- max. elevation 2000 m above sea level

- max. relative humidity 80 % up to 31 °C; linearly decreasing down to 50 %

relative humidity at 40 °C.

admissible temperature of the environ
ment

dimensions 240 mm x 284 mm x 375 mm (H x W x D)

weight without rotor 14.5 kg

maximum speed 13,000 rpm (hematocrit rotor 12,000 rpm)

maximum RCF value 16,060 (fixed-angle rotor)

minimum speed 1600 rpm

minimum RCF value 209

noise <64 dB (A)

maximum capacity 24 x 2 ml

4 °C to 35 °C during operation

-10 °C to 50 °C during storage and transport

14,926 (hematocrit rotor)

maximum permissible load 24 x 4 g

maximum permissible unbalance 1 g

50

Technical data

Parameter Value/description

maximum kinetic energy 2.000 kNm

compliance with standards Manufactured and checked in accordance with

EN 61 010-1, EN 61 010-2-020, EN 50 081-1, EN 50 082-1.

Electrical connections/fuses

Order no.. Voltage Frequency max. current Power consumption Fuses inside

instrument *

# 7500 3635 230 V 50/60 Hz 1.25 A 170 W 2 x 6.3 AT (5 x 20 mm)

# 7500 3557 120 V 50/60 Hz 2.5 A 170 W 2 x 6.25 AT (6.3 x 32 mm)

1 x 10 AT (6.3 x 32 mm)

51

Speed / g diagram for rotor 7500 3325

100000

13000

10000

1000

Drehzahl [1/min] – speed [rpm]

r = 5,9 cm

min

r = 8,5 cm

max

52

100

10 100 1000 10000

100000

16060

RZB [x g] – RCF [x g]

Autoclaving protocol

(only fixed-angle rotor 7500 3325)

1

2

3

4

5

6

7

8

9

10

Date Remark Operator Signature

53

Index

A

acceleration 8
accessories

additional 11
standard 9

acoustic signal

for stopping 32

aerosol tightness

check 27
aluminum rotor: 39
approved rotor 22
autoclaving 41
autoclaving cycle

permissible maximum 41

C

capillaries

placing 19
centrifuge

starting 31
centrifuge tubes 8
cleaning 37

conditions of warranty 42
contamination

necessary measures 38
continuous operation 30, 33
control panel

readings 8
corrosive substances

protective vessels for 4

D

damage

symbol for potential 5
dangerous chemicals 3
decontamination 38
density

maximum of sample 25
disinfectant 39
disinfection 4

procedure 39
disinfection with bleaching lye 40
display

during run 31
displays

brief failure 43

not lit 43

54

E

EC Guidelines 5
emergency lid release 18
Emergency lid release 7
error code

"br" 44
"Lid" 45
"OPEN" 45
E-1 or E-12 44
E-3 44

F

fastening nut

for hematocrit rotor 20

fine tuning

run time 30
speed setting 29

fixed-angle rotor 11

adapters 13
optional accessory 24
technical data 12

fluorescein

test solution for aerosol tightness 27

fuses 50

H

hazardous substances 3
hazards

symbols used for 5

hematocrit

measurement 35
standardization 35

hematocrit determination

quick run function not to be used 33

hematocrit dish

standard equipment 9

hematocrit rotor

fastening 20
insertion 19
loading 19
removal 20
technical data 10

hints

symbol for 5

I

icons

for denoting dangers and potential damage 5

infectious material

precautions in case of tube breakage 38

installation

55

place of 15

K

key

"start" 31

keys

general operation 8

L

lid

blockage 43

lid lock

built-in safety system 7

lid open during run

warning 7

lid release

emergency 7, 18
manual 18

M

mains connection 15
mains switch

not present 17

maintenance 37
manual lid release 18

steps for 18
maximum sample density 3
maximum sample volume 8

O

operation

continuous 30, 33

preselected run time 30

short-time 33
organic solvents

not allowed for cleaning 38
original parts

mandatory use 4
overloading

dangers implied 25

P

partial loading

of rotor 28
pathogenic microorganisms

protection against 4
permissible volume

calculation of for dense samples 25

56

preset values

display 32

problems

handling of 43

protective vessels

for corrosive substances 4

Q

quick run 8

not for hematocrit measurements 33
quick run function 33
quick run key 8

R

radius of centrifugation

for calculation of RCF value 34
RCF value 10, 34
reading harp 13

for hematocrit 35

standard accessory 9
reading spiral 13

for hematocrit 35

optional accessory 9
readings

of control panel during run 8

relative centrifugal force 34
rotor

approved 22
insertion 22
loading 28
partial loading 28
removal 24

rotor cap 23, 24

mandatory fastening 20

rotor chamber

technical data 47

rotor insertion

tools for 22

run time

fine tuning 30
for hematocrit measurements 30
range 30

setting 30
running noise 44
RZB-Wert 12

S

safety instructions 3, 4
safety measures 3
safety standards 5
safety systems

built-in 7
safety zone 3

57

30 cm around centrifuge 15

sample

maximum density 25
volume reduction for dense samples 25

sample density

maximum 3

sample volume

maximum 8
service 42
service contracts 42
setting

run time 30

speed 29
settings

change during run 31
short-time operation 33
site of installation 15
sodium fluorescein

test solution for aerosol tightness 27
software check

internal 17
speed

fine tuning 29

range 29

setting 29
speed of centrifugation

for calculation of RCF value 34
standardization

of hematocrit measurements 35
standby mode 7

start key 31
starting run 31
stopping 32
substructure 15
symbols

for hazards and dangers 5

T

temperature regulation 8
tools for inserting rotor 22
toxins

protection against 4

transport

precautions for 17

transport protection

removal 15

tube

breakage with infectious material 38

tubes

for centrifugation 8
types 11
volume range 11

V

volume

58

calculation of permissible v. for dense samples 25

volume of sample

maximum 8

W

warning

lid open during run 7

warranty conditions 42

59

For Ordering or Technical Information

Asia Pacific Kendro Laboratory Products (H.K.) Limited · Hong Kong · Tel. [phone] · Fax [phone] · [e-mail]

Europe, Middle East, Africa

Kendro Laboratory Products International Sales · Langenselbold · Germany · Tel. [phone] · Fax [phone] ·

[e-mail]

Latin America
North America

Internet http://www.kendro.com

Kendro Laboratory Products International Sales · Asheville, NC · USA · Tel. [phone] · Fax [phone] · [e-mail]..com
Kendro Laboratory Products · Asheville, NC · USA · Tel. [phone] · Fax [phone] · [e-mail]

Kendro Laboratory Products GmbH
Robert-Bosch-Straße 1
D-63505 Langenselbold
Telephone: [phone]

In the interest of continuous product development, we
reserve the right to make changes without express
notice.

20056895 Bio haemo_uk 11/03

Printed in Germany