Hanna Instruments HI 84529-01 User Manual
Instruction Manual
HI 84529
Titratable Acidity Mini Titrator &
pH Meter for Dairy Products
Dear
Customer
Thank you for choosing a Hanna Instrument product.
Please read this instruction manual carefully before using this instrument.
This manual will provide you with the necessary information for correct use
of this instrument, as well as a precise idea of its versatility.
If you need additional technical information, do not hesitate to e-mail us
at tech@hannainst.com or view our worldwide contact list for a Hanna
representative near you at www.hannainst.com.
All rights are reserved. Reproduction in whole or in part is prohibited without
the written consent of the copyright owner, Hanna Instruments Inc.,
Woonsocket, Rhode Island, 02895 , USA
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Included ....................................................................................................................... 4
Safety Measures .........................................................................................................5
Description .............................................................................................................. 6-8
Signi cance of Use ...................................................................................................................................6-7
Principle of Operation ..............................................................................................................................7-8
Diagram ................................................................................................................. 9-12
Product Diagram .................................................................................................................................... 9-10
Keypad Function .........................................................................................................................................11
Guide to Indicators ......................................................................................................................................12
Setup/ Installation .............................................................................................13-24
Dosing Pump ................................................................................................................................................13
Titrator Startup ...........................................................................................................................................13
Startup Menu ........................................................................................................................................ 14-19
Guide to Display Codes ........................................................................................................................19-20
Pump Calibration Messages ...............................................................................................................20-21
pH Calibration Messages .......................................................................................................................... 22
Titration Messages ..............................................................................................................................22-23
Electrode Preparation Procedure. ..................................................................................................... 24-25
Operational Guide ..............................................................................................25-44
Electrode Calibration Procedure........................................................................................................25-29
pH Bu er Temperature Dependence .....................................................................................................29
Dosing Pump Installation. ......................................................................................................................... 30
Dosing Pump Prime Procedure ..........................................................................................................31-32
Pump Calibration Procedure...............................................................................................................32-34
Titration Procedure .............................................................................................................................35-38
Tips for an Accurate Measurement. ..................................................................................................38-39
Titrator GLP Information. .......................................................................................................................... 40
pH Measurement. ............................................................................................................................... 40-43
pH Meter GLP Information. .......................................................................................................................43
PC Interface and Data Transfer ................................................................................................................44
Maintenance .......................................................................................................45-47
Electrode Conditioning and Maintenance..............................................................................................45
Troubleshooting Guide ........................................................................................................................46-47
Speci cations ....................................................................................................48-49
Accessories .........................................................................................................50-51
Warranty .................................................................................................................... 52
Table of
Contents
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Included
Please examine this product carefully. Make sure that the instrument is not
damaged. If any damage occurred during shipment, please notify your local
Hanna Representative.
Each HI 84529 mini titrator is supplied complete with:
HI 84529-70 Reagent kit for titratable acidity in dairy products
FC 260B pH electrode
HI 5315 Reference electrode
HI 7662-M Temperature probe
HI 7072 Fill solution (30 mL)
HI 700640 Cleaning solution for milk deposits (2 x 20 mL)
One capillary dropper pipette
Two 100 mL beakers
Tube set (aspiration tube with titrant bottle cap and dispensing tube with tip)
Dosing Pump Valve
5 mL Syringe
1 mL Plastic pipette
Stir bar
Power adapter
Instruction manual
Note: Save all packing material until you are sure that the instrument works
correctly. Any defective item must be returned in its original packing.
Included
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Before using this product, make sure that it is entirely suitable for your
speci c application and for the environment in which it is used.
Operation of this instrument may cause unacceptable interferences to other
electronic equipment, thus requiring the operator to take all necessary steps
to correct interferences. Any variation introduced by the user to the supplied
equipment may degrade the instrument’s EMC performance.
To avoid damages or burns, do not put the instrument in microwave ovens.
For your and the instrument’s safety, do not use or store the instrument in
hazardous environments.
Safety
Measures
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Safety Measures
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Description
The HI 84529 is a low-cost easy to use, microprocessor-based automatic
minititrator and pH meter designed for the rapid and accurate analysis of
Total Titratable Acidity in Dairy Products. The HI 84529 will quickly become
a valuable tool by eliminating subjective factors including color indicators,
errors in calculations or erratic titrant additions.
The instrument bene ts from Hanna’s many years of experience as a
manufacturer of quality analytical instrumentation. A clear and well-designed
user interface makes the instrument intuitive and simple to use.
By pressing the Start key in Titrator mode, the instrument will automatically
titrate the sample to the set end point, perform all necessary calculations and
display the results in the selected unit. At the end of the titration, another
titration can be started by pressing the Restart key. A dedicated HELP keys
aids in setup, calibration and troubleshooting.
Other features:
pH meter / mV meter
Stir speed control
Graphic mode to display the titration data
Data can be stored using the log feature and then exported to a USB stick or
transferred to a PC using the USB connection
Log on demand for up to 400 samples (200 pH/mV measurements; 200 for
titration results)
GLP feature, to view calibration data for the pH electrode and pump
Signi cance
of Use
Description
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Titratable acidity can be expressed in several units; % lactic acid (% l.a.),
degree Soxhlet Henkel (°SH), degree Dornic (°D) or degree Thörner (°TH).
Each of these units corresponds to a speci c procedure used to titrate dairy
products.
Soxhlet Henkel degrees (°SH) - mostly used in Central Europe exept France
and the Netherlands. This value is a number of mL of 0.25N NaOH used for
titration of 100 mL milk, using phenolphtalein as indicator.
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Thorner degrees (°Th) - mostly used in Sweden and the CIS. This value is
obtained by titrating 100 mL of milk, diluted with 2 parts of distilled water,
with 0.1N NaOH, using phenolphtalein as indicator.
Dornic degrees (°D) - mostly used in Netherlands and France. This value is
obtained by titrating 100 mL of milk, with N/9 (0.11 N) NaOH, using phenolphtalein
as indicator.
Percent lactic acid (%l.a.) - frequently used in the UK, USA, Canada, Australia
and New Zealand. This value is obtained in the same way as °D, dividing the
result by 100.
The titratable acidity values will vary depending on the method used. Select
Low 50 to titrate a non-diluted sample, or select Low 20 / High 20 to titrate
20 mL or 20 g samples that are diluted with twice its volume of deionized a
distilled water. The HI 84529 uses methods based on AOAC International
and Standard Methods for the Examination of Dairy Products. Both of these
method report titratable acidity as % lactic acid, a rough conversion factor
can be used to convert the results to the other available units:
From: To: Divide By:
%l.a. °SH 0.0225
%l.a. °D 0.0100
%l.a. °TH 0.0090
Signi cance
of Use
The HI 84529 can be customized to meet the needs of any dairy analysis
lab. Samples can be titrated by weight or volume, diluted or non-diluted (low
range only) and titrated to a xed pH end point that can be adjusted by the
user.
The methodology for titratable acidity in dairy products is based on
neutralization reaction where the acids in the sample (i.e. lactic acid) reacts
with a base (i.e. sodium hydroxide) to produce water:
H++OH¯—>H2O
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Principle of
Operation
Description
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Principle of
Operation
In an ideal solution, the endpoint of an acid titration corresponds
stoichiometrically to the complete neutralization of the acids present.
This endpoint for this reaction can be determined visually using a color
indicator (i.e. phenolphthalein), however this endpoint becomes very
objective in opaque and colored samples. The HI 84529 removes this issue
by titrating to a xed pH endpoint that can be set by the user. The HI 84529
can be customized to meet individual needs with samples titrated by mass or
by volume, in two di erent ranges, with diluted and non-diluted (low range
only). For precise analysis the sample size, volume of titrant added and titrant
concentration must be known.
The HI 84529 Titratable Acid in Dairy Products Minititrator utilizes a simple
sample preparation, a high quality dosing pump for titrant additions,
potentiometric endpoint determination and instantaneous computations.
To maintain the high accuracy of the minititrator a simple pump calibration
is required. The pump calibration uses a known quantity of a known solution
to compensate for changes in the dosing system, this procedure should be
performed regularly.
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Description
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1. Titrant bottle
2. Liquid Crystal Display (LCD)
3. Keypad
4. Electrode holder
5. Dispensing tube
Product
Diagram
Overhead View
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Front View
Product Diagram
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Product
Diagram
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3
4
Product Diagram
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Rear View
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6
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1. Dispensing tube
2. pH Electrode
3. Temperature probe
4. Reference electrode
5. Syringe
6. Electrode holder
7. Power switch
8. USB connector (PC interface)
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9. USB connector
(Storage interface)
10. Power adapter
11. BNC electrode connector
12. Temperature connector
13. Reference connector
14. Titrant bottle
15. Dosing Pump Valve
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Keypad
Function
ESC - used to leave the current
screen and to return either to the
previous screen or to the main
screen. In Setup, exits a parameter
without changing the value
/ - used to modify the parameters’
values, to scroll the information
displayed while viewing a help
screen or to move between the
options from the instrument’s Setup
CAL - used to access the Electrode
and Pump calibration options
HELP - used to access/exit the
instrument’s contextual help
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LOG - used to save the current pH/
mV reading in pH meter mode and
the titration result
MENU -used to enter Setup, Recall
or GLP selection menu, while
instrument is in pH or titration mode
STIR - used to start/stop the stirrer
Note: The stirrer starts automatically
during pump calibration and
titration, it cannot be stopped by
pressing STIR key.
Product Diagram
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Guide to
Indicators
During the instrument’s operation a set of information are displayed on
the LCD. Displayed icons:
Stirrer on Pump running Unstable reading
Stirrer is not working properly Parameter can be modi ed
1. Current time and instrument mode
information (pH meter or Titrator)
2. Procedural Indicators
3. Instrument status
4. Virtual option keys
5. Stirrer and reading status
6. Main reading information
7. pH temperature compensation
mode (Manual or Automatic)
8. Temperature reading
Product Diagram
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Note: If temperature probe is not connected, Manual Temperature Compensation
is used and MTC appears on the right side of the screen. If Automatic Temperature
Compensation is in use the ATC appears on the right side of the screen.
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The dosing pump is based on a valve that automatically moves the titrant
between the titrant bottle and syringe when lling the syringe and between
the syringe and sample when dispensing. A replaceable 5 mL plastic syringe is
used to limit the amount of titrant used per test to ensure the highest possible
accuracy. Before a set of titrations, it is necessary to prime the dosing system.
Note: Once titrations have been completed, the dosing system should be
cleaned with deionized water using the prime feature.
Dosing
Pump
This is a general outline of the steps required to perform a titration. The
following topics are expanded upon in each section that follows.
1. Place the instrument on a at table. Do not place the instrument in direct sun
light.
2. Connect the power adapter to the instrument.
3. Turn the instrument ON using the power switch on the rear panel of the
instrument.
4. Set up the instrument. See the “Setup Menu” section for details.
5. Connect the pH electrode to the instrument.
6. Connect the reference electrode to the instrument. Connect the
temperature probe to the instrument.
7. Calibrate the pH electrode.
8. Connect the tubes and the valve. See the “Dosing Pump Installation”
section for the procedure.
9. Remove the titrant bottle cap and replace it with the bottle cap with
tubes. Place the titrant bottle in the appropriate place on the titrator top.
Note: Di erent titrants are required based on the concentration. See
“Pump Calibration Procedure” for details.
10. Prime the syringe. To assure high accuracy, verify there are no air bubbles
in the syringe or tubing. Calibrate the pump.
Note: Di erent volumes of standards are required based on the
concentration. See “Pump Calibration Procedure” for details.
11. Prepare the sample.
12. Run a titration and log sample results.
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Titrator
Startup
Setup/Installation
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Startup
Menu
The titrator’s setup menu may be accessed from the main screen or titration
screens (meter or titrator) by pressing the MENU key, then SETUP. A list of setup
parameters will be displayed with currently con gured settings. While in the
setup menu, it is possible to modify the instrument’s operation parameters.
The ARROW keys permit the user to scroll the setup parameters. Press HELP to
view the contextual help. Press ESC to return to the main screen.
Concentration Unit
% l.a., °SH, °D, °TH
Press the corresponding virtual option
key to change the option.
% l.a. - % lactic acid; °SH - °Soxhlet
Henkel; °D - °Dornic; °TH - °Thörner
Acid Titration
Low 20, Low 50 or High 20
Press the corresponding virtual option
key to select the desired range.
Note: Di erent titrants are required for
each range.
Sample Type
Mass or Volume.
Samples can be added by volume or by
mass. For improved accuracy all samples
should be added by mass.
Setup/Installation
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Calibration Timeout
Disabled or 1 to 7 days. This option is used
to set the number of days before the pH
calibration expired warning message is
displayed. Press MODIFY to access the
calibration timeout screen.
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Use the ARROW keys to select the value.
Press Accept to con rm or ESC to return
to the setup menu without saving the
changes.
pH Resolution
0.1 or 0.01.
Press the displayed virtual option key to
change the option.
Temperature Unit
°C, °F or K.
Press the virtual option key to change the
option.
Time
Press the Modify key to change the time
and time format
Startup
Menu
Press Format to switch between 12
hour (am/pm) and 24 hour mode. Press
the arrow to highlight the value to be
modi ed. Use the ARROW keys to change
the value. Press Accept to con rm the
new value or ESC to return to the setup.
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Setup/Installation
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Startup
Menu
Date
Press the MODIFY key to change the date
and date format.
Key Beep
Select ENABLE to activate or DISABLE
to deactivate the Key Beep function. If
enabled, a short beep will be heard every
time a key is pressed.
Error Beep
Select ENABLE to activate or DISABLE to
deactivate the Error Beep function.
If enabled, a beep will be heard when an
error condition occurs.
Decimal Separator
Select the symbol used for a decimal
separator. You can chose either period or
a comma.
Setup/Installation
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LCD Contrast
This option is used to set the display’s
contrast. Press MODIFY to change the
display’s contrast. The default value is 8.
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Use the ARROW keys or ← / → to increase/
decrease the value. Press ACCEPT to
con rm the value or ESC to return to the
setup menu.
LCD Backlight
Press Modify to change the backlight
level. The default value is 3.
Use the ARROW keys or ← / → to increase/
decrease the backlight level.
Press ACCEPT to con rm or ESC to return
to the setup menu.
pH endpoint
Press Modify to change the pH endpoint
value.
The default value is 8.3 pH.
Startup
Menu
Use the ARROW keys to select the value.
Press Accept to con rm or ESC to return
to the setup menu without saving the
changes.
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Setup/Installation
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