Hanna Instruments C 214 User Manual

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MANC214R3 10/04

Instruction Manual

C 214

Multiparameter Bench

Photometer

for

Wastewater Treatment

Application

www.hannainst.com

These Instruments are in

Compliance with the CE Directives

Dear Customer,
Thank you for choosing a Hanna product. Please read this instruction

manual carefully before using the meter. This manual will provide you
with the necessary information for the correct use of the instrument. If
you need additional technical information, do not hesitate to e-mail us
at [e-mail]. These instruments are in compliance with

directives.

TABLE OF CONTENTS

PRELIMINARY EXAMINATION ........... 3

ABBREVIATIONS ............................. 3

GENERAL DESCRIPTION ................... 4

SPECIFICATIONS ............................. 5

PRECISION AND ACCURACY ............. 5

PRINCIPLE OF OPERATION ............... 6

FUNCTIONAL DESCRIPTION ..............8

GUIDE TO DISPLAY CODES .............. 9

TIPS FOR AN ACCURATE

MEASUREMENT ............................12

PARAMETERS REFERENCE TABLES ..17

HEALTH & SAFETY ........................19

OPERATIONAL GUIDE .................... 20

AMMONIA LOW RANGE ................. 22

AMMONIA HIGH RANGE ................ 25

CHLORINE, FREE ...........................28

CHLORINE, TOTAL ......................... 31

NITRATE ....................................... 34

NITROGEN, TOTAL LR .................... 37

NITROGEN, TOTAL HR ................... 42

OXYGEN DEMAND, CHEMICAL LR ....47

OXYGEN DEMAND, CHEMICAL MR .. 50
OXYGEN DEMAND, CHEMICAL HR ...53

PHOSPHORUS, REACTIVE .............. 56

PHOSPHORUS, ACID HYDROLYZABLE ...

PHOSPHORUS, TOTAL ................... 63

PHOSPHORUS, REACTIVE HR .........67

PHOSPHORUS, TOTAL HR ..............70

INTERFACE WITH PC ..................... 74

STANDARD METHODS ................... 76

BATTERY REPLACEMENT ................ 76

ACCESSORIES ............................... 77

CE DECLARATION OF CONFORMITY .... 78

WARRANTY ...................................79

HANNA LITERATURE ......................79

WARRANTY

All Hanna Instruments meters are warranted for two years against
defects in workmanship and materials when used for its intended
purpose and maintained according to the instructions.

This warranty is limited to repair or replacement free of charge.
Damages due to accident, misuse, tampering or lack of prescribed

maintenance are not covered.
If service is required, contact your dealer. If under warranty, report the

model number, date of purchase, serial number and the nature of the
failure. If the repair is not covered by the warranty, you will be notified
of the charges incurred.

If the instrument is to be returned to Hanna Instruments, first obtain a
Returned Goods Authorization Number from the Customer Service
Department and then send it with shipment costs prepaid. When
shipping any instrument, make sure it is properly packaged for complete

59

protection.
To validate your warranty, fill out and return the enclosed warranty

card within 14 days from the date of purchase.

Hanna Instruments reserves the right to modify the design, construction
and appearance of its products without advance notice.

HANNA LITERATURE

Hanna publishes a wide range of catalogs and handbooks for an
equally wide range of applications. The reference literature currently
covers areas such as:

All rights are reserved. Reproduction in whole or in part is prohibited without
the written consent of the copyright owner, Hanna Instruments Inc.,
Woonsocket, Rhode Island, 02895 , USA.

2

• Water Treatment

• Process

• Swimming Pools

• Agriculture

• Food

• Laboratory

and many others. New reference material is constantly being added to
the library.

For these and other catalogs, handbooks and leaflets contact your
dealer or the Hanna Customer Service Center nearest to you. To find
the Hanna Office in your vicinity, check our home page at
www.hannainst.com.

79

CE DECLARATION OF CONFORMITY

Recommendations for Users

Before using these products, make sure that they are entirely suitable for your specific application
and for the environment in which they are used.

Operation of these instruments may cause unacceptable interferences to other electronic equip­ments, this requiring the operator to take all necessary steps to correct interferences.

Any variation introduced by the user to the supplied equipment may degrade the instruments' EMC
performance.

To avoid damages or burns, do not put the instrument in microwave ovens. For yours and the
instrument safety do not use or store the instrument in hazardous environments.

PRELIMINARY EXAMINATION

Please examine this product carefully. Make sure that the instrument
is not damaged. If any damage occured during shipment, please
notify your Dealer.

Each Meter is supplied complete with:

• Five Sample Vials

• Two 9 V Batteries

• 12 VDC Transformer (HI 710005 or HI 710006)

• Instruction Manual

Note: Save all packing material until you are sure that the instrument

works correctly. Any defective item must be returned in its
original packing with the supplied accessories.

ABBREVIATIONS

°C: degree Celsius
COD: Chemical Oxygen Demand
EPA: US Environmental Protection Agency
°F: degree Fahrenheit
g/L: grams per liter. g/L is equivalent to ppt (part per thoushand)
HR: high range
LR: low range
mg/L: milligrams per liter. mg/L is equivalent to ppm (part per million)
mL: milliliter
MR: medium range
µg/L: micrograms per liter. µg/L is equivalent to ppb (part per billion)

78

3

GENERAL DESCRIPTION

ACCESSORIES

C 99 & C 200 Series is a line of 17 different bench, microprocessor­based photometers to measure

more than 50 different parameters in
water and wastewater. These multipurpose meters are manufactured
to measure the most important parameters of the application they
have been especially designed for:
C 99 Laboratories, with COD C 200 Laboratories

C 203 Aquaculture C 205 Boilers & Cooling Towers
C 206 Environmental Testing C 207 Industrial Wastewater
C 208 Water Conditioning C 209 Education
C 210 Pulp & Paper Mills C 211 Chemical Manufacturers
C 212 Power Plant Utilities C 213 Municipal Wastewater
C 214 Wastewater Treatment Applic. C 215 Nutrient Analyses
C 216 Pool & Spa Applic. C 218 Environmental Applic.
C 226 Pool & Spa Applic.

The reagents are in liquid or powder form and are supplied in bottles
and ready-to-use vials or in packets. The amount of reagent is precisely
dosed to ensure the maximum repeatability.

Display codes aid the user in routine operations.
The meters have an auto-shut off feature that will turn off the instrument

after 10 minutes of non-use.
The C 99 & C 200 Series can be connected to a personal computer via

the HI 920010 three wire RS 232 cable. The HI 92000 Hanna

®

Windows

Compatible Software aids the user to manage all test data.

REAGENT SETS

HI 93701-01 100 free chlorine tests
HI 93701-03 300 free chlorine tests
HI 93711-01 100 total chlorine tests
HI 93711-03 300 total chlorine tests
HI 93754A-25 25 COD LR tests
HI 93754B-25 25 COD MR tests
HI 93754C-25 25 COD HR tests
HI 93758A-50 50 reactive phosphorus tests
HI 93758B-50 50 acid hydrolyzable phosphorus tests
HI 93758C-50 50 total phosphorus tests
HI 93763A-50 50 reactive phosphorus HR tests
HI 93763B-50 50 total phosphorus HR tests
HI 93764A-25 25 ammonia LR tests
HI 93764B-25 25 ammonia HR tests
HI 93766-50 50 nitrate tests
HI 93767A-50 50 total nitrogen LR tests
HI 93767B-50 50 total nitrogen HR tests

OTHER ACCESSORIES
C115-00300 5 mL graduated syringe
C9800-01 Hanna Reactor (115 VAC)
C9800-02 Hanna Reactor (230 VAC)
HI 710005 110VAC to 12VDC voltage adapter
HI 710006 220VAC to 12VDC voltage adapter
HI 721310 9V battery (10 pcs)

HI 731311 glass vials with cap (5 pcs)
HI 731318 Tissue for wiping cuvets (4 pcs)
HI 731340 200 µL automatic pipette

HI 731341 1000 µL automatic pipette
HI 731342 2000 µL automatic pipette
HI 731350 tips for 200 µL automatic pipette (25 pcs)
HI 731351 tips for 1000 µL automatic pipette (25 pcs)
HI 731352 tips for 2000 µL automatic pipette (4 pcs)

HI 740142 1 mL graduated syringe
HI 740143 1 mL graduated syringe (6 pcs)
HI 740144 Pipette tip (6 pcs)

HI 740157 Plastic refilling pipette (20 pcs)
HI 740216 Test tube cooling rack (25 holes)
HI 740217 Laboratory bench safety shield
HI 92000 Windows

®

Compatible Software

HI 920010 PC connection cable
HI 93703-50 Cuvets cleaning solution (230 mL)

4

77

STANDARD METHODS

SPECIFICATIONS

Description Range Method

Ammonia LR 0.00 to 3.00 mg/L Nessler
Ammonia HR 0 to 100 mg/L Nessler
Chlorine, Free 0.00 to 5.00 mg/L DPD
Chlorine, Total 0.00 to 5.00 mg/L DPD
Nitrate 0.0 to 30.0 mg/L Chromotropic Acid
Nitrogen, Total 0.0 to 25.0 mg/L Chromotropic Acid
Nitrogen, Total HR 10 to 150 mg/L Chromotropic Acid
COD LR 0 to 150 mg/L Dichromate, Mercuric Sulphate
COD MR 0 to 1500 mg/L Dichromate, Mercuric Sulphate
COD HR 0 to 15000 mg/L Dichromate, Mercuric Sulphate
Phosphorus, Reactive 0.00 to 5.00 mg/L Ascorbic Acid
Phosphorus, Acid Hydrolyzable 0.00 to 5.00 mg/L Ascorbic Acid
Phosphorus, Total 0.00 to 3.50 mg/L Ascorbic Acid
Phosphorus, Reactive HR 0.0 to 100.0 mg/L Vanadomolybdophosphoric Acid

Phosphorus, Total HR 0.0 to 100.0 mg/L Vanadomolybdophosphoric Acid

BATTERY REPLACEMENT

Besides the 12 VDC supply, this meter can also be fed by two 9V
batteries.

To prolong battery life, switch your meter off after use. However, the
meter has an auto-shut off feature that will turn itself off after 10
minutes of non-use.

A blinking "LOBAT" on the display, while a
measurement is in progress, indicates low
voltage and the batteries should be replaced.

If the batteries are not replaced immediately,
in order to prevent erroneous readings due to
low voltage, "-BA-" is displayed soon
afterwards. At this point the batteries must be
changed.

Battery replacement must only take place in a non-hazardous area
using two 9V alkaline batteries.

Remove the battery cover on the back of photometer, attach two fresh
9V batteries, while paying attention to the correct polarity, and replace
the cover.

The meter will turn on automatically when a
new battery is connected. You can turn it off
by pressing ON/OFF.

Light Life Life of the instrument
Light Detector Silicon Photocell
Environment 0 to 50°C (32 to 122°F);

max 90% RH non-condensing

Power Supply 2 x 9 V batteries / 12 to 20 VDC through

voltage adapter

Auto-Shut off After 10' of non-use
Dimensions 230 x 165 x 70 mm (9.0 x 6.5 x 2.8")
Weight 640 g (22.6 oz.)

For specifications related to each single parameter (e.g. range, precision,
etc.), refer to the related measurement section.

PRECISION AND ACCURACY

Precision is how closely repeated measurements agree with each other.
Precision is usually expressed as standard deviation (SD).

Accuracy is defined as the nearness of a test result to the true value.
Although good precision suggests good accuracy, precise results can be

inaccurate. The figure explains these definitions.
For each parameter, the precision is expressed in the related measurement

section as standard deviation at a specific concentration value of the
analite. The standard deviation is obtained with a single instrument using

a representative lot of reagents.

76

5

PRINCIPLE OF OPERATION

Absorption of Light is a typical phenomenon of interaction between
electromagnetic radiation and matter. When a light beam crosses a
substance, some of the radiation may be absorbed by atoms, molecules
or crystal lattices.
If pure absorption occurs, the fraction of light absorbed depends both
on the optical path length through the matter and on the physical­chemical characteristics of substance according to the Lambert-Beer
Law:

-log

I

A ελc d

Where:

-log

=Absorbance (A)

I/I

o

=intensity of incident light beam

I

o

=intensity of light beam after absorption

I

ελ=molar extinction coefficient at wavelength λ

c =molar concentration of the substance
d =optical path through the substance

Therefore, the concentration "c" can be calculated from the absorbance
of the substance as the other factors are known.

Photometric chemical analysis is based on the possibility to develop
an absorbing compound from a specific chemical reaction between
sample and reagents.

Given that the absorption of a compound strictly depends on the
wavelength of the incident light beam, a narrow spectral bandwidth
should be selected as well as a proper central wavelength to optimize
measurements.

The optical system of Hanna's C 99 & C 200 Series multiparameter
photometers is based on special subminiature tungsten lamps and
narrow-band interference filters to guarantee both high performance
and reliable results.

Four measuring channels (at four different wavelengths) allow a wide
range of tests.

ελc d

/I

o

or

/PUP - Program Up
/PDN - Program Down
/PTM - Turn Test Mode On
/Brx - Set the baud rate

1 - 300 2 - 600

3 - 1200 4 - 2400
/KBL - Lock Keyboard
/KBU - Unlock Keyboard
?PR# - Send Current Program Number
?BRQ - Send current baud rate

1 - 150 2 - 300

3 - 600 4 - 1200

5 - 2400
?CNQ - Send Concentration (three bytes)

conc

decimal point

unit

m - ppm b - ppb
t - ppt u - pcu
h - pH

?ERR - send error / status information

0 - No error

1 - CAP

2 - HI

3 - ZERO

4 - LO

5 - IDLE

6 - ZERO DONE

7 - TIMED READ

C 200 Series block diagram (optical layout)

6

75

INTERFACE WITH PC

To connect your meter to the PC use the optional HI 920010 (available
from your Hanna Dealer). Make sure that your meter is switched off
and plug the connectors, one into the meter RS 232C socket, the other
into the serial port of your PC.

Note: Cables other than HI 920010 may use a different configuration,

in which case, communication between the meter and the PC
may not be possible.

SETTING THE BAUD RATE

The transmission speed (baud rate) of the meter and the external
device must be identical. The meter is factory set to 2400.

If you wish to change this value, please contact your nearest Hanna
Center.

SENDING COMMANDS FROM PC

With terminal programs such as, for example, Telix®, Windows Termi­nal®, it is possible to remotely control your Hanna Instruments bench
meter. Use HI 920010 cable to connect the meter to the PC, start the
terminal program and set the communication options as follows: 8, N,
1, no flow control.

Command Types

To send a command to the meter the scheme is:

<DLE> <command> <CR>

This line makes the computer send a Data Link Escape character (/ or
?), the command expressed as a number or a 3-character sequence
and a CR character.

Note: Windows Terminal® and all the other terminal programs that

support the ANSI escape sequence, represent the DLE character
by the string ‘^P’ and the CR character by the string ‘^M’.

Type of Commands

/OFF - Turn the meter OFF
/PDR - Press Read Direct
/PTR - Press Timer
/PZR - Press Zero

A microprocessor controlled special tungsten lamp emits radiation
which is first optically conditioned and beamed to the sample contained
in the vial. The optical path is fixed by the diameter of the vial. Then
the light is spectrally filtered to a narrow spectral bandwidth, to obtain
a light beam of intensity

or

I

I

.

o

The photoelectric cell collects the radiation I that is not absorbed by
the sample and converts it into an electric current, producing a potential
in the mV range.

The microprocessor uses this potential to convert the incoming value
into the desired measuring unit and to display it on the LCD.

The measurement process is carried out in two phases: first the meter
is zeroed and then the actual measurement is performed.

The vial has a very important role because it is an optical element and
thus requires particular attention. It is important that both the
measurement and the calibration (zeroing) vials are optically identical
to provide the same measurement conditions. Most of parameters use
the same vial for both. In each case, insert the test vials always in the
same position into the holder.

It is necessary that the surface of the vial is clean and not scratched.
This is to avoid measurement interference due to unwanted reflection
and absorption of light. It is recommended not to touch the vial walls
with hands.

Furthermore, in order to maintain the same conditions during the
zeroing and the measuring phases, it is necessary to close the vial to
prevent any contamination.

Excel© Copyright of "Microsoft Co."

Lotus 1-2-3© Copyright of "Lotus Co."

Windows® and Windows Terminal® are registered Trademark of "Microsoft Co."

TELIX® is registered Trademark of "Deltacomm"

74

7

FUNCTIONAL DESCRIPTION

FRONT PANEL

1) Vial Holder

2) Dual Level Liquid Crystal Display (LCD)

3) Programs List

4) READ DIRECT, to perform measurement immediately

5) TIMER, to perform measurement after a preprogrammed
countdown

6) ZERO, to zero the meter prior to measurement

7) Program t and s, to select the desired parameter

8) ON/OFF, to turn the meter on and off

REAR PANEL

1) Power Supply 12 VDC 2.5 Watt

2) RS 232 Socket

3) Batteries Compartment

• Wait for a few seconds and the

display will show “-0.0-”. Now the
meter is zeroed and ready for
measurement.

• Remove the blank vial.

• Place the sample vial into the holder

and push it completely down.

• Press READ DIRECT and "SIP" will blink on the display.

• The instrument directly displays concentration in mg/L of phosphate

3-

(PO

) on the Liquid Crystal Display.

4

The method detects free (orthophosphate), condensed inorganic
forms (meta-, pyro- and other polyphosphates) and organic forms
of phosphates present in the sample.

• To convert the reading to mg/L of P2O5 , multiply by a factor of

0.748.

• To convert the reading to mg/L of phosphorus (P) concentration,
multiply by a factor of 0.326.

Note: for accurate measurements

1) wash glassware only with phosphate-free detergents

2) clean all glassware with 1 : 1 hydrochloric acid solution and rinse with

deionized water.

INTERFERENCES

Arsenate: positive error
pH: the sample should have a neutral pH
Temperature: the method is temperature sensitive.

It is recommended to add the Molybdovanadate Reagent
and to run measurements at T = 20 to 25 °C:
T < 20 °C causes a negative error
T > 25 °C causes a positive error

Turbidity: turbidity and suspended matter in large amounts may

cause interference because the strongly acidic reaction
conditions may dissolve suspended matter or cause
desorption of phosphates from particles. Before
measurement, turbidity or suspended matter should
be removed by treatment with active carbon and by
prior filtration.

8

73

Phosphorus, Total HR

• At the end of the digestion place the

vials carefully in the test tube rack and
allow to cool to room temperature.
Warning: as the vials are still hot,
be careful in handling them.

• Select the program number corresponding to Total Phosphorus High
Range on the secondary LCD by pressing PROGRAM t and s.

• Remove the cap from the vials and add exactly 2.0 mL of Sodium
Hydroxide (NaOH) Solution 1,54 N to each vial, while keeping
the vials at a 45-degree angle. Replace the cap tightly and mix
by inverting the vials a couple of times.

• Remove the cap from the vials and add exactly 0.5 mL of HI
93763B-0 Molybdovanadate Reagent to each vial, while keeping
the vial at a 45-degree angle. Replace the cap tightly and mix by
inverting the vials a couple of times.

GUIDE TO DISPLAY CODES

Note: The secondary LCD below shows a generic "P– –", whereas the

meter will indicate the exact program number (e.g. "P1" for
Ammonia LR).

This indicates that the meter is in a ready
state and zeroing can be performed.

Sampling in progress. This flashing prompt
appears each time the meter is performing
a measurement.

The microprocessor is adjusting the light
level, indicated by a scrolling "SIP".

This indicates that the meter is in a zeroed
state and measurement can be performed.

The instrument is performing an internal
check-up.

• Place the blank vial into the holder
and push it completely down.

• Press TIMER and the display will show the countdown prior to the
measurement. Alternatively, wait for 7 minutes and press ZERO. In
both cases "SIP" will blink during measurement.

Phosphorus, Total HR

72

The blinking "LOBAT" indicates that the
battery voltage is getting low and the
batteries need to be replaced.

This indicates that the batteries are dead
and must be replaced.

Light over range. The vial is not inserted
correctly and an eccess ambient light is
reaching the detector. If the vial is properly
inserted, then contact your dealer or the
nearest Hanna Customer Service Center.

9

ERROR MESSAGES
a) on zero reading:

The lamp is not working properly. Contact
your dealer or the nearest Hanna Customer
Service Center.

The lamp is not working properly. Contact
your dealer or the nearest Hanna Customer
Service Center.

This indicates that the meter has lost its
configuration. Contact your dealer or the
nearest Hanna Customer Service Center.

This indicates that the zeroing procedure
failed due to a low signal-to-noise ratio. In
this case press ZERO again.

The instrument cannot adjust the light level.
Please check that the vial does not contain
any debris.

Reagent Blank Correction: This method needs a reagent blank
correction. A single blank vial may be used more than once; the blank
vial is stable up to one day (room temperature). For most accurate
measurement, run a blank for each set of measurements and always use
the same lot of reagents for blank and samples.

• Preheat the Hanna Reactor C 9800 to 150 °C (302°F). For correct

use of the reactor follow Reactor Instruction Manual.
Use of the optional HI 740217 safety shield is strongly recommended.
Do not use an oven or microwave because leaking samples can
generate a corrosive and possibly explosive atmosphere.

• Remove the cap from two Reagent Vials.

• Add exactly 5.0 mL of sample to one vial (sample vial), and 5.0
mL of deionized water to the other vial (blank vial), while keeping
the vials at a 45-degree angle.

b) on sample reading:

There is not enough light to perform a
measurement. Please check the preparation
of the zero vial.

There is too much light to perform a
measurement. Please check the preparation
of the zero vial.

There is too much light for the sample
measurement. Please check if the right
sample vial is inserted.

The sample and zero vial are inverted.

10

• Add the content of one packet of Potassium Persulfate for Phosphorus
analysis to each vial. Replace the cap tightly and shake gently the
vials until all the powder is

completely dissolved.

• Insert the vials into the reactor and
heat them for 30 minutes at 150°C.

71

Phosphorus, Total HR

PHOSPHORUS, TOTAL HIGH RANGE

SPECIFICATIONS

Range 0.0 to 100.0 mg/L (as PO
Resolution 0.1 mg/L
Precision ±3.0 @ 75.0 mg/L
Typical EMC ±0.1 mg/L

Deviation
Light Source Tungsten lamp with narrow band interference filter

@ 420 nm

Method Adaptation of the Standard Methods for the

Examination of Water and Wastewater, 20
edition, 4500-P C, vanadomolybdophosphoric acid

method. A persulfate digestion converts organic
and condensed inorganic forms of phosphates to
orthophosphate. Then the reaction between
orthophosphate and the reagents causes a yellow
tint in the sample.

REQUIRED REAGENTS

Code Description Q.ty/test Q.ty/set

* Reagent Vial 1 vial 50 vials

--- Deionized Water 5 mL 1 bottle

--- Potassium Persulfate 1 packet 50 packets

--- NaOH Solution 1.54 N 2 mL 1 bottle
HI 93763B-0 Molybdovanadate Reagent 0.5 mL 1 bottle

* Reagent Vial identification: P, white cap.
Note: Store the unused vials in their container in a cool and dark place.

3-

)

4

A zero reading was not taken. Follow the
instruction described in the measurement
procedures for zeroing the meter.

Under range. A blinking "0.00" indicates
that the sample absorbs less light than the
zero reference. Check the procedure and
make sure that you use the same vial for
reference (zero) and measurement (unless
a reagent blank correction procedure is
prescribed).

th

1) A flashing value of the maximum
concentration indicates an over range
condition. The concentration of the sample
is beyond the programmed range: dilute
the sample and rerun the test.

2) A flashing value lower than the maximum
concentration indicates a low signal-to­noise ratio condition. In this case accuracy
of the result is not guaranteed. Repeat the
reading procedure.

REAGENT SET

HI 93763B-50 Reagents for up to 49 tests

For other accessories see page 77.

REQUIRED ACCESSORIES

C 9800-01 Hanna reactor (115 VAC)
C 9800-02 Hanna reactor (230 VAC)
HI 740216 test tube cooling rack (25 holes)
HI 740217 Laboratory bench safety shield

For other accessories see page 77.

MEASUREMENT PROCEDURE

Before starting to use the reagent kit it is recommended to read carefully

all the instructions and the Health & Safety Data Sheet
(HSDS). Pay particular attention to all warnings, cautions
and notes. Failure to do so may result in serious injury to
the operator.

Phosphorus, Total HR

70

11

TIPS FOR AN ACCURATE MEASUREMENT

The instructions listed below should be carefully followed during testing
to ensure most accurate results.

COLLECTING AND MEASURING SAMPLES

• For adding the exact amount of sample or liquid reagent to the
reagent vials it is strongly recommended to use the available
Hanna automatic fixed-volume pipettes or class A laboratory pipettes
(symbolized like a generic pipette tip in the following parameter
related chapters):

pipette code volume

HI 731340 200 µL
HI 731341 1000 µL
HI 731342 2000 µL

• Place the sample vial into the holder

and push it completely down.

• Press READ DIRECT and "SIP" will blink on the display.

• The instrument directly displays concentration in mg/L of phosphate

3-

(PO

) on the Liquid Crystal Display.

4

• To convert the reading to mg/L of P2O5 , multiply by a factor of

0.748.

• To convert the reading to mg/L of phosphorus (P) concentration,
multiply by a factor of 0.326.

For correct use of the automatic pipette, please follow the related
Instruction Sheet.

Alternatively, it is possible to use the optional HI 740142 1 mL
graduated syringe or C115-00300 5 mL graduated syringe. For
the correct use of the syringes, see instructions below.

1. In order to measure exactly e.g. 5 mL of reagent with the

syringe:
(a) push the plunger completely into the syringe and insert the

tip into the solution.

(b) pull the plunger up until the lower edge of the seal is exactly

on the 5 mL mark.

(c) take out the syringe and clean the outside of the syringe tip.

Be sure that no drops are hanging on the tip of the syringe, if
so eliminate them. Then, keeping the syringe in vertical
position above the vial, push the plunger completely down
into the syringe. Now the exact amount of 5 mL has been
added to the vial.

( a

)

( b

)

(c)

5 mL

Note: for accurate measurements

1) wash glassware only with phosphate-free detergents

2) clean all glassware with 1 : 1 hydrochloric acid solution and rinse with

deionized water.

INTERFERENCES

Interference may be caused by:
Bismuth: negative error
Fluoride: negative error
pH: the sample should have a neutral pH
Sulfide: negative error

To eliminate sulfide: add Bromine Water drop-wise
until a pale yellow color develops; remove Bromine
Water excess by adding Phenol solution drop-wise.

Temperature: the method is temperature sensitive.

It is recommended to run measurements at T = 20 to 25 °C:
T < 20 °C causes a negative error
T > 25 °C causes a positive error

Turbidity: turbidity and suspended matter in large amounts may

cause interference because the strongly acidic reaction
conditions may dissolve suspended matter or cause
desorption of phosphates from particles. Before
measurement, turbidity or suspended matter should
be removed by treatment with active carbon and by
prior filtration.

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Phosphorus, Reactive HR