Hach DR 2800 User Manual

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DOC022.53.00720

DR 2800

USER MANUAL

January 2008, Edition 2

Section 1 Specifications ........................................................................................................................................ 7

Section 2 General Information .............................................................................................................................. 9

2.1 Safety information .............................................................................................................................................. 9

2.1.1 Use of hazard information ......................................................................................................................... 9

2.1.2 Precautionary labels ................................................................................................................................. 9

2.1.3 Class 1 LASER ......................................................................................................................................... 9

2.1.4 Chemical and Biological Safety .............................................................................................................. 10

2.2 Overview of product ......................................................................................................................................... 10

Section 3 Installation ........................................................................................................................................... 11

3.1 Unpack the instrument ..................................................................................................................................... 11

3.2 Operating environment .................................................................................................................................... 11

3.3 Power connections ........................................................................................................................................... 12

3.4 Connection ....................................................................................................................................................... 13

3.5 Cell compartments, Cell adapters, Light Shield and Protective Cover ............................................................. 14

3.5.1 Cell compartments and adapters ............................................................................................................ 14

3.5.2 Installation of the cuvette/sample cell adapters ...................................................................................... 15

3.5.3 Use of the light shield for measurements ................................................................................................ 16

3.5.4 Protective Cover ..................................................................................................................................... 18

3.6 Mobile use of the DR 2800 for field analysis .................................................................................................... 19

3.6.1 Position the Protective Cover .................................................................................................................. 20

3.7 Beam path ........................................................................................................................................................ 21

Section 4 Start Up ................................................................................................................................................ 23

4.1 Power the instrument on and off ...................................................................................................................... 23

4.2 Language selection .......................................................................................................................................... 23

4.3 Self-Check ....................................................................................................................................................... 23

Section 5 Standard Operations ........................................................................................................................... 25

5.1 Overview .......................................................................................................................................................... 25

5.1.1 Tips for the use of the touch screen ........................................................................................................ 25

5.1.2 Use of the alphanumeric keypad ............................................................................................................ 25

5.1.3 Main Menu .............................................................................................................................................. 26

5.2 Instrument Setup mode .................................................................................................................................... 27

5.2.1 Operator ID ............................................................................................................................................. 27

5.2.2 Sample ID ............................................................................................................................................... 28

5.2.3 Date and time .......................................................................................................................................... 29

5.2.4 Display and sound preferences .............................................................................................................. 29

5.2.5 Power Management ................................................................................................................................ 29

5.2.6 PC and printer ......................................................................................................................................... 31

5.2.6.1 Printer setup ............................................................................................................................... 31

5.2.6.2 Print data .................................................................................................................................... 33

5.2.6.3 HACH Data Trans ....................................................................................................................... 33

5.2.7 Password ................................................................................................................................................ 34

5.2.7.1 Password deactivation ................................................................................................................ 35

Table of Contents

5.3 Store, recall, send and delete data .................................................................................................................. 37

5.3.1 The data log ............................................................................................................................................ 37

5.3.1.1 Auto/manual data storage ........................................................................................................... 37

5.3.1.2 Recall stored data from the data log ........................................................................................... 37

5.3.1.3 Send data from the data log ....................................................................................................... 38

5.3.1.4 Delete stored data from the data log .......................................................................................... 39

5.3.2 Time Course ........................................................................................................................................... 40

5.3.2.1 Data Storage from Time Course ................................................................................................. 40

5.3.2.2 Recall Stored Data from Time Course ........................................................................................ 40

5.3.2.3 Send Data from Time Course ..................................................................................................... 41

5.3.2.4 Delete Stored Data from Time Course ....................................................................................... 42

5.4 Stored Programs .............................................................................................................................................. 43

5.4.1 Select a saved test/method; enter user-specific basic data .................................................................... 43

5.4.2 Stored program options .......................................................................................................................... 43

5.4.3 Use of program timers ............................................................................................................................ 45

5.4.4 Set the dilution factor .............................................................................................................................. 46

5.4.5 Run a standard adjust ............................................................................................................................. 46

5.4.6 Set the chemical form ............................................................................................................................. 47

5.4.6.1 Change of the default setting of the chemical form .................................................................... 48

5.4.7 Run a reagent blank ................................................................................................................................ 48

5.4.8 Analysis of samples ................................................................................................................................ 49

5.4.9 Add stored programs to the favorite programs list .................................................................................. 50

5.5 Barcode Programs ........................................................................................................................................... 51

5.5.1 Complete a barcode 13 mm test/vial ...................................................................................................... 51

5.5.2 Select the measuring range .................................................................................................................... 52

5.5.3 Select the chemical evaluation form ....................................................................................................... 52

5.5.3.1 Change of the default setting of the chemical form .................................................................... 53

5.5.4 Basic test-specific and sample-specific data settings ............................................................................. 53

5.5.5 Sample blank .......................................................................................................................................... 54

5.5.6 Update/edit barcode tests ....................................................................................................................... 55

5.5.6.1 Manual update of a barcode test ................................................................................................ 55

5.5.6.2 Update an existing barcode test ................................................................................................. 58

5.5.6.3 Program a new test ..................................................................................................................... 59

5.5.7 Upgrade of the instrument software ........................................................................................................ 59

Section 6 Advanced Operations ......................................................................................................................... 61

6.1 User Programs ................................................................................................................................................. 61

6.1.1 Program a user method .......................................................................................................................... 61

6.1.1.1 Single wavelength settings ......................................................................................................... 63

6.1.1.2 Multi wavelength settings ............................................................................................................ 64

6.1.1.3 Calibration settings for single and multi wavelength mode ......................................................... 66

6.1.1.4 Store a user program .................................................................................................................. 70

6.1.1.5 Additional user-defined parameters and functions ..................................................................... 7

6.1.2 Free programming program type ............................................................................................................ 72

6.1.2.1 Measurement process ................................................................................................................ 72

6.1.2.2 Enter a new element of a measuring sequence ......................................................................... 73

6.1.2.3 Enter the calibration formula (evaluation formula) ...................................................................... 75

6.1.2.4 Enter variables ............................................................................................................................ 78

6.1.2.5 Save a free programming user program ..................................................................................... 78

6.1.3 Select a user program ............................................................................................................................. 79

6.1.4 Add, edit and delete user programs from the favorites list ...................................................................... 79

6.1.4.1 Add to Favorites .......................................................................................................................... 80

6.1.4.2 Edit .............................................................................................................................................. 80

6.1.4.3 Delete ......................................................................................................................................... 80

Table of Contents

6.2 Favorite Programs ........................................................................................................................................... 81

6.2.1 Recall a favorite program ........................................................................................................................ 81

6.2.2 Delete a favorite program ....................................................................................................................... 81

6.3 Standard Addition – monitoring/checking results ............................................................................................. 82

6.3.1 Complete a standard addition ................................................................................................................. 83

6.4 Single Wavelength (absorbance, concentration and transmittance measurements) ....................................... 87

6.4.1 Set up single wavelength mode .............................................................................................................. 87

6.4.2 Take single wavelength measurements .................................................................................................. 89

6.5 Multi-Wavelength mode – measurements at more than one wavelength ........................................................ 90

6.5.1 Set the reading mode at different wavelengths ....................................................................................... 90

6.5.2 Complete a measurement in the multi wavelength mode ....................................................................... 93

6.6 Time course of absorbance/transmittance ....................................................................................................... 94

6.6.1 Time course setup parameters ............................................................................................................... 94

6.6.2 Time course scan reading ....................................................................................................................... 95

6.6.3 Analysis of time course data ................................................................................................................... 96

6.6.3.1 Navigation of a time scan or a time scan analysis ......................................................................97

6.7 System checks ................................................................................................................................................. 97

6.7.1 Instrument information ............................................................................................................................ 98

6.7.2 Upgrade of the instrument software ........................................................................................................ 98

6.7.3 Optical checks ......................................................................................................................................... 99

6.7.3.1 Wavelength check ...................................................................................................................... 99

6.7.3.2 Stray light check ....................................................................................................................... 100

6.7.3.3 Absorbance check .................................................................................................................... 101

6.7.3.4 Verification kit ........................................................................................................................... 102

6.7.4 Output checks ....................................................................................................................................... 104

6.7.5 Lamp history ......................................................................................................................................... 104

6.7.6 Factory service ...................................................................................................................................... 104

6.7.7 Service time .......................................................................................................................................... 105

6.7.8 Instrument Backup ................................................................................................................................ 106

Section 7 Maintenance ...................................................................................................................................... 109

7.1 Cleaning requirements ................................................................................................................................... 109

7.1.1 Spectrophotometer ............................................................................................................................... 109

7.1.2 Display .................................................................................................................................................. 109

7.1.3 Cuvettes/sample cells ........................................................................................................................... 109

7.2 Insert or change of the battery ....................................................................................................................... 110

7.2.1 Information about using the battery ...................................................................................................... 111

7.2.1.1 Dispose of the lithium battery ................................................................................................... 111

7.2.1.2 Optimal operation of the battery ............................................................................................... 112

7.2.1.3 Lifespan of the battery .............................................................................................................. 112

7.2.1.4 Load the battery/Operating time ............................................................................................... 112

7.3 Lamp replacement ......................................................................................................................................... 113

Section 8 Troubleshooting ................................................................................................................................ 117

Section 9 Replacement Parts ............................................................................................................................ 119

Section 10 Contact Information ........................................................................................................................ 121

Section 11 Limited Warranty ............................................................................................................................. 123

Index .................................................................................................................................................................... 125

Table of Contents

Section 1 Specifications

Specifications are subject to change without notice.

Performance Specifications

Operating Mode Transmittance (%), Absorbance and Concentration

Source Lamp Gas-filled Tungsten (visible)

Wavelength Range 340–900 nm

Wavelength Accuracy ± 1.5 nm

Wavelength Reproducibility < 0.1 nm

Wavelength Resolution 1 nm

Wavelength Calibration Automatic

Wavelength Selection Automatic, based on method selection

Spectral Bandwidth < 8 nm

Photometric measuring range ± 3.0 Ext in Wavelength Range 340–900 nm

Photometric Accuracy

Photometric Linearity

Stray Light

Data storage 500 measured values (result, date, time, sample ID, user ID)

5 mAbs at 0.0–0.5 Abs 1% at 0.50–2.0 Abs

< 0.5%–2 Abs < = 1% at > 2 Abs with neutral glass at 546 nm

< 0.2% T @ 340 nm with KV450/3 < 0.1% T @ 340 nm with NaNO

User programs 50

Physical and Environmental Specifications

Width 220 mm (8.6 in)

Height 135 mm (5.3 in)

Depth 330 mm (12.9 in)

Weight

Operating Requirements 10–40 °C (50–104 °F), max. 80% relative humidity (non-condensing)

Storage Requirements -40–60 °C (-40–140 °F) max. 80% relative humidity (non-condensing)

Additional technical data

Mains connection

Interfaces

Enclosure Rating

Safety class Safety class II

4.06 kg (8.95 Ibs) without battery

4.38 kg (9.66 Ibs) with battery

15 V- / 30VA Plug-in power supply unit: (100–240 V/50–60 Hz)

Use only shielded cable with max. length of 3 m. 1 x USB type A 1 x USB type B

IP 41 with closed lid IP 42 with Protective Cover in place

Specifications

Section 2 General Information

2.1 Safety information

Please read this entire manual before unpacking, setting up or operating this equipment. Pay attention to all danger, warning and caution statements. Failure to do so could result in serious injury to the operator or damage to the equipment.

Make sure that the protection provided by this equipment is not impaired, do not use or install this equipment in any manner other than that specified in this manual.

2.1.1 Use of hazard information

DANGER Indicates a potentially or imminently hazardous situation which, if not avoided, will result in death or serious injury.

WARNING Indicates a potentially or imminently hazardous situation which, if not avoided, could result in death or serious injury.

CAUTION Indicates a potentially hazardous situation that may result in minor or moderate injury.

2.1.2 Precautionary labels

This symbol, if noted on the instrument, references the instruction manual for operation and/or safety information.

Electrical equipment marked with this symbol may not be disposed of in European public disposal systems after 12 August of 2005. In conformity with European local and national regulations (EU Directive 2002/96/EC), European electrical equipment users must now return old or end-of life equipment to the Producer for disposal at no charge to the user.

Note: For return for recycling, please contact the equipment producer or supplier for instructions on how to return end-of-life equipment, producer-supplied electrical accessories and all auxiliary items for proper disposal.

This symbol indicates that the instrument contains a Class 1 LASER device.

LASER CLASS 1

Data: 0.3 mW; λ = 650 nm

Important Note: Indicates a situation which, if not avoided, may cause damage to the instrument. Information that requires special emphasis.

Note: Information that supplements points in the main text.

Read all labels and tags attached to the instrument. Personal injury or damage to the instrument could occur if not observed. A symbol, if noted on the instrument, will be included with a danger or caution statement in the manual.

2.1.3 Class 1 LASER

A Class 1 LASER is installed in this instrument. Class 1 LASERS are products where the radiant power of the LASER beam accessible (the accessible emission) is always below the Maximum

General Information

2.1.4 Chemical and Biological Safety

Permissible Exposure value. Therefore, for Class 1 LASERS the output power is below the level at which it is believed eye damage will occur. Exposure to the beam of a Class 1 LASER will not result in eye injury. Class 1 LASERS may therefore be considered safe. However, Class 1 LASER products may contain LASER systems of a higher Class but there are adequate engineering control measures to ensure that access to the beam is not reasonably likely. Examples of such products include LASER printers and compact disc players. CDRH assession number 0510555-02.

Data: 0.3 mW; wavelength = 650 nm

DANGER Potential Chemical/ Biological Exposure Hazards. Handling chemical samples, standards and reagents can be dangerous. Users of this product are advised to familiarize themselves with safety procedures and the correct use of chemicals, and to carefully read all relevant Material Safety Data Sheets.

Normal operation of this instrument may involve the use of hazardous chemicals or biologically harmful samples.

2.2 Overview of product

• The user must observe all cautionary information printed on the

original solution containers and safety data sheet prior to their use.

• All waste solutions must be disposed in accordance with local and national law.

• The type of protective equipment must be selected according to the concentration and amount of the dangerous substance at the specific workplace.

The DR 2800 spectrophotometer is a VIS spectrophotometer with a wavelength range of 340 to 900 nm. The instrument comes with a complete set of application programs and multi-language support.

The DR 2800 spectrophotometer contains the following application modes: Stored Programs (pre-installed tests), Barcode Programs, User Programs, Favorite Programs, Single Wavelength Mode, Multi-Wavelength Mode and Time Course Mode.

The DR 2800 spectrophotometer provides digital readouts in direct concentration units, absorbance, or percent transmittance.

When a user-generated or programmed method is selected, the on-screen menus and prompts direct the user through the test.

This menu system also can be used to generate reports, statistical evaluations of generated calibration curves, and to report instrument diagnostic checks.

Section 3 Installation

3.1 Unpack the instrument

WARNING Electrical and Fire Hazards. Use only the provided power supply. Only qualified personnel should conduct the tasks described in this section of the manual.

The DR 2800 Spectrophotometer comes packaged with the following items:

• DR 2800 spectrophotometer

• Plug-in power supply, including 4 adapter for EU, GB, USA,

AUS/China

• Dust cover

• 3 different cuvette/cell adapters (A, B and C)

• Light Shield belongs to standard configuration of DR 2800

• Protective Cover

• DR 2800 User Manual

• CD-ROM containing the HACH and HACH LANGE procedures

3.2 Operating environment

• Quick start guide

Note: If any of these items are missing or damaged, contact the manufacturer or a sales representative immediatly.

The following conditions are necessary to ensure correct instrument operation and accurate results:

• Place the instrument firmly on an even surface. Do not push any objects under the instrument.

• Maintain an ambient temperature of 10 to 40 ºC (50 to 104 ºF) for proper instrument operation.

• The relative humidity should be less than 80%; moisture should not condense on the instrument.

• Leave at least a 15 cm (6 in.) clearance at the top and on all sides for air circulation to avoid overheating of electrical parts.

• Do not operate or store the instrument in extremely dusty, damp or wet locations.

• Keep the surface of the instrument, the cell compartment and all accessories clean and dry at all times. Splashes or spills on and in the instrument should be cleaned up immediately (see

section 7.1 on page 109).

Important Note: Protect the instrument from temperature extremes, including heaters, direct sunlight and other heat sources.

Installation

3.3 Power connections

Install the correct adapter plug on the supplied external power supply (Figure 1) by sliding the adapter on until it "clicks" into position. Correctly mounted, both housing of power supply and plug are in line. Plug the external power supply cord into the connector on the back panel of the instrument, then plug the supply into a power outlet (100–240 V~ / 50–60 Hz). Press the power switch on the back of the instrument to initialize power (Figure 2 on page 13).

Figure 1 Power adapter

1 Power supply with EU adapter plug installed 3 USA adapter plug 2 UK adapter plug 4 AUS/China adapter plug

3.4 Connection

Installation

The DR 2800 has two USB interfaces as a standard feature, located on the back of the instrument (Figure 2).

The USB Type A interface is used for communications with a printer, USB memory stick, barcode scanner, or keyboard. A USB memory stick is used to update instrument software.

The USB Type B interface is used for communications with a PC. The optional Hach Data Trans software (see section 5.2.6.3 on

page 33) must be installed on the PC for this use.

A USB hub may be used to connect several accessories at a time.

Note: USB cables must not be longer than 3 meters (10 feet).

These USB interfaces enable data and graphics to be output to a Printer and a PC and upgrade instrument software (see

section 6.7.2 on page 98).

Figure 2 Interfaces

1 On/Off switch 4 Cover 2 USB type B 5 USB type A 3 Plug in power supply

Installation

3.5 Cell compartments, Cell adapters, Light Shield and Protective Cover

3.5.1 Cell compartments and adapters

The DR 2800 has two cell compartments (Figure 3). Only one cuvette/sample cell type at a time can be used for a measurement.

Cell compartment #1

• 13-mm and 16-mm round cuvettes/vials

Note: Cell compartment #1 contains a barcode reader for cuvettes/vials.

Cell compartment #2

Cell compartment #2 uses adapters to accommodate different cuvette/sample cell types.

• 1-inch square or 50-mm rectangular cuvettes/cells (can be inserted directly into the cell compartment without using an adapter).

• Adapter A: 10-mm square cuvettes/cells

• Adapter B: Pour-Thru cells (refer to the instruction sheet

supplied with the Pour-Thru cell) and multi-path cuvettes/cells

Note: Pour-Thru Cell must be used with Adapter B, not Adapter C.

• Adapter C: One-inch round cuvettes/cells and AccuVac® Ampules

Note: One-inch round cuvettes/cells and AccuVac Ampules must be used with Adapter C, not Adapter B.

Figure 3 Cell compartments

1 Cell compartment #1 2 Cell compartment #2

Installation

Figure 4 Cuvette/Sample cell adapters

1 10 mm square cuvette/sample cell adapter (A) 3 1 inch round cuvette/sample cell adapter (C) 2 1 inch Pour-Thru adapter (B)

3.5.2 Installation of the cuvette/sample cell adapters

1. Open the cell compartment.

2. Select the correct adapter for the cuvette/sample cell type.

3. Insert the adapter so the arrow on top of the adapter points to

the left (Figure 5 on page 16) and the orientation tab fits the groove in the compartment opening. The cuvette/sample cell type imprint should be legible on the adapter (Figure 4).

Note: The arrow on top of the adapter indicates the direction of the light beam path.

Installation

Figure 5 Installation of a cuvette/sample cell adapter

3.5.3 Use of the light shield for measurements

The light shield (Figure 6 on page 17) prevents light interference when using 13 mm and 16 mm vial tests and must be in place before measurements can be taken in cell compartment #1. The light shield is required only when using 13 mm or 16 mm vial tests.

Installation

The DR 2800 is shipped with the light shield installed. Remove the light shield before using cell compartment #2. The light shield can be stored in the Protective Cover (Figure 8 on page 18)

Figure 6 Light Shield

Installation of the light shield

1. Open the cell compartment.

2. Insert the light shield so the arrow on the light shield points to

the left and the orientation tab fits the groove in the compartment opening (Figure 7).

1 Light Shield

Figure 7 Light Shield in place

Installation

3.5.4 Protective Cover

Figure 8 Protective Cover

1 Protective Cover 3 Protective Cover with inserted cuvette/sample cell 2 Protective Cover (inside view)

The inside of the Protective Cover is intended to house the cuvette/sample cell adapters. The recesses for holding the cuvette/sample cell adapters are marked with the corresponding letters.

adapter A in position A.

3.6 Mobile use of the DR 2800 for field analysis

If the DR 2800 spectrophotometer is used to carry out field analyzes, it may be necessary to take a number of measures to compensate for the fluctuating ambient light conditions.

You should take the measures described below if the following warning is displayed after you take a measurement:

Error: Too much ambient light! Move device into shade or close the lid!

General measure:

Shield the instrument from the sun by standing so that your shadow falls on it, and carry out the measurement again. If the warning appears again, take the following measures.

Measures:

Measurements in a 10 mL round cuvette/cell, a rectangular cuvette/cell or AccuVac Ampules

Close the lid of the cuvette compartment and carry out the measurement.

Measurements in a 13 or 16 mm round cuvette/vial or a 25 mL square cuvette/cell

To obtain consistent measurement conditions in all weather conditions, place the Protective Cover on the open cuvette/cell compartment (see Figure 9 Position the Protective Cover on page

20) and carry out the measurement.

Installation

Important Note: When you carry out measurement in 25 mL square cuvettes/cells, take care that there is no adapter in the Protective Cover.

The enclosure rating of the photometer increases from IP41 to IP42 when the Protective Cover is in place.

Installation

3.6.1 Position the Protective Cover

Figure 9 Position the Protective Cover

1. After you have inserted the zero solution cell or the sample cell, position the Protective Cover over the open cell compartment. The lettering "Protective Cover" must be readable from the display side of the instrument.

2. Press the Protective Cover lightly into the correct position until the cell compartment is completely sealed and the Protective Cover cannot slip off.

3. Carry out the measurement.

Note: The Protective Cover can simultaneously be used to house the different cuvette adapters. The cuvette adapter recesses in the Protective Cover are marked with the corresponding letters and arrows of the adapter. The arrows in the Protective Cover and on the adapters indicate the direction of insertion.

3.7 Beam path

Installation

Figure 10 shows the beam path of the DR 2800:

Figure 10 Beam path

1 Tungsten lamp 8 Splitter mirror 2 Entrance slit 9 Reference-element 3 Heat-protection glass 10 Cell compartment #2 4 Grating 11 Lens 5 Exit slit 12 Measurement element 6 Lens 13 Cell compartment #1 7 Filter wheel 14 LED

Installation

Section 4 Start Up

4.1 Power the instrument on and off

1. Plug in to the power supply (laboratory analysis) or insert the

battery (field analysis).

2. The push-button switch on the back of the instrument switches the instrument on (press for about 1 second) and off (press for about 3 to 5 seconds). An acoustic signal confirms that the instrument has been switched off.

Note: Do not turn the instrument off and on in rapid succession. Always wait about 20 seconds before turning the instrument on again, otherwise the electronic and mechanical systems will be damaged.

4.2 Language selection

The spectrophotometer software includes several language options. The first time the instrument is turned on, the language selection screen will appear.

4.3 Self-Check

1. Select the desired language.

2. Press OK to confirm the language selection. The self check will

start automatically.

Change the language setting

The instrument functions in the selected language until the option is changed.

1. While turning the instrument on, touch the screen at any point until the list for selecting a language appears (about 30 seconds).

2. Select the required language.

3. Press OK to confirm. The test program subsequently starts

automatically.

Each time the instrument is powered up, a series of diagnostic tests are performed automatically to ensure operation of major system components.

This procedure, which takes approximately two minutes, checks the system, lamp, filter adjustment, wavelength calibration and voltage. Each test which functions correctly is confirmed with a check mark.

The Main Menu is displayed when power up diagnostics are completed.

Start Up

If the instrument detects any deviation relative to the last calibration, a system check has to be carried out.

4. Remove the cuvette from the cell compartment and close the lid.

5. Then press Start.

The system check is carried out (duration approx. 6 minutes).

Note: Further error messages during self check, see Section 8 on page

117.

Section 5 Standard Operations

5.1 Overview

5.1.1 Tips for the use of the touch screen

The entire screen is touch-activated. To make a selection, press the screen with a fingernail, fingertip, pencil eraser or a stylus. Do not press the screen with a sharp object, such as the tip of a ball point pen.

• Do not place anything on top of the screen, to prevent damage or scratching on the screen.

• Press keys, words or icons to select them.

• Use scroll bars to move up and down long lists very quickly.

Press and hold the scroll bar, then move up or down to move through the list.

• Highlight an item from a list by pressing it once. When the item has been successfully selected, it will be displayed as reversed text (light text on a dark background).

5.1.2 Use of the alphanumeric keypad

This display is used to enter letters, numbers and symbols as needed when programming the instrument. Unavailable options are disabled (grayed out). The icons on the right and left of the screen are described in Table 1.

The central keypad changes to reflect the chosen entry mode. Press a key repeatedly until the desired character appears on the screen. A space can be entered by using the underscore on the YZ_ key.

Note: A USB keyboard (with US keyboard layout) or a USB Barcode handset scanner can be used for input (see Section 9 on page 119).

Table 1 Alphanumeric keypad

Icon / key Description Function

ABC/abc Alphabetic

# % Symbols Punctuation, symbols and numerical sub- and superscripts may be entered.

123 Numeric For entering regular numbers.

CE Clear Entry Clear the entry.

Left Arrow Backspace

Right Arrow Advance

When entering alphabetic characters (ex. user-entered units), this key allows to toggle between upper and lower case letters.

Moves back one position. This deletes the character previously entered in the new position.

Moves to the next space in an entry when two adjacent characters occur on the same key.

Standard Operations

5.1.3 Main Menu

Option Function

A variety of modes may be selected from the ”Main Menu“. The following table briefly describes each menu option.

Table 2 ”Main Menu“ Options DR 2800

Stored Programs / Barcode Programs (HACH LANGE Programs)

User Programs

Favorite Programs List of methods/tests created by the user to suit his own requirements.

Single Wavelength

Multi Wavelength

Time Course The time scan records the absorbance or % transmittance at a wavelength over a defined time.

System Checks

Recall Data Stored data can be called up, filtered, transmitted and deleted.

Instrument Setup

Stored programs are pre-programmed methods that make use of HACH reagents and LANGE cuvette tests and pipette tests.

The DR 2800 Procedures Manual contains illustrated, step-by-step procedures for analyzes using HACH programs. The working procedures for LANGE tests are included in the test packs.

User programs make "made to measure analysis" possible:

- Users can program methods they have developed themselves

- Existing HACH and LANGE methods can be stored as user programs. The LANGE tests can then be modified to suit the user's requirements.

Single wavelength measurements are: Absorbance measurements: The light absorbed by the sample is measured in absorbance

units. Transmittance measurements (%): The percentage of the light that passes through the sample

and reaches the detector is measured. Concentration measurements: A concentration factor can be entered to enable the measured

absorbance values to be converted into concentration values. In the multi-wavelength mode, absorbance (Abs) or percentage transmittance (%T) is measured

at up to four wavelengths, and absorbance differences and absorbance relationships are calculated. Simple conversions into concentrations can also be carried out.

The system checks menu offers a number of options, including optical checks, output checks, lamp history, instrument update, service time and instrument backup.

In this mode, user-specific or method-specific settings can be entered: Operator-ID, Sample-ID, Date & Time, Display & Sound, Power Management, PC & Printer and Password.

5.2 Instrument Setup mode

5.2.1 Operator ID

Standard Operations

1. Select Instrument Setup in the ”Main Menu“.

A selection of functions appears in order to configure the functions of the instrument.

Use this option to enter up to 30 sets of operator initials (up to 5 characters each) into the instrument. This feature helps record which operator measured each sample.

1. Press Operator ID in the Instrument Setup.

2. Press New to enter a new Operator ID.

3. Use the alphanumeric keypad to enter a new Operator ID.

4. Press OK to confirm.

5. The display shows the chosen Operator ID.

6. Press OK. The instrument will return to the Instrument Setup

screen and show the selected operator identifier.

7. The chosen Operator ID is activated.

Note: Press Delete to remove an Operator ID from the list.

Note: Alternatively, enter or change an Operator ID in measurement mode.

In the results screen, press Options>More>Instrument Setup or if an Operator ID is already assigned, select the "Operator ID symbol immediately in the results screen.

Standard Operations

5.2.2 Sample ID

Use this option to enter up to 100 Sample Identification tags (up to 13 characters each) into the instrument. Sample IDs can be used to specify the sample location or other sample specific information.

1. Press Sample ID in the Instrument Setup.

2. Press New to enter a new Sample ID.

3. Use the alphanumeric keypad to enter a new Sample ID.

Note: If a USB Barcode handset scanner ( see Section 9 on page 119) is connected, Sample IDs can also be scanned. Sample IDs can also be entered with a USB keyboard.

4. Press OK to confirm.

5. To number the Sample IDs sequentially (e.g. Inflow (01 etc.)), select Add Number.

• Use the arrow keys to specify the first number of the

sequence.

• Use the key between the arrow keys to enter the first

number of the sequence using the alphanumeric keypad.

6. Press OK to return to "Instrument Setup".

7. The Sample ID is activated. Each Sample ID is automatically

numbered in ascending order after a measurement. The number is shown in parentheses behind the Sample ID.

Note: To remove a Sample ID, highlight the ID and press Delete.

Note: A Sample ID can be entered or changed in measurement mode. In

the results screen, press Options>More>Instrument Setup. If a Sample ID is already assigned, select the Sample ID symbol in the results screen.

5.2.3 Date and time

1. Press Date & Time in the Instrument Setup.

2. The date and time are subdivided over a number of fields.

3. Press OK to confirm. The instrument will return to

5.2.4 Display and sound preferences

1. Press Display & Sound in the Instrument Setup.

Standard Operations

Press the appropriate field and use the arrow keys to change the value.

Instrument Setup.

5.2.5 Power Management

The following options will be displayed:

• Display Contrast—Adjusts the display brightness to suit lighting conditions.

• Screen touch—Activates//Deactivates a short beep each time the screen is pressed (Default:off).

• Reading done—Activates/Deactivates a sound when a reading is complete (Default: short beep every time a reading is complete).

•Timer—Adjusts the length of the timer sound. Select Short or Long. Long beeps are recommended for noisy environments.

2. Press OK to confirm. The instrument will return to Instrument Setup.

The DR 2800 can run on mains or battery power.

Note: The battery is not part of the standard scope of delivery.

1. Select Power Management in the ”Instrument Setup“ menu.

The battery symbol indicates the charge status of the battery in %.

Note: The timer settings in the Power Management menu are only active when the instrument is running on battery power.

Standard Operations

2. Select one of the options under Standby mode to set the

length of the idle period that can elapse before the instrument switches to the Standby mode when running on battery power.

Note: In standby mode, the backlighting of the screen is switched off. Touching the display causes the lighting to switch on again.

3. Select one of the options under Auto off to set the length of the idle period that can elapse before the instrument automatically switches off when running on battery power.

Note: After the instrument switches itself off automatically, you have to press the push-button switch on the back of the instrument to start it again.

5.2.6 PC and printer

USB Interfaces Description

USB (Type B)

USB (Type A)

This USB interface is only intended to connect the instrument to a PC (with installation of the HACH Data Trans Software).

This USB port can be used to connect a printer, a USB memory stick , a barcode scanner or a keyboard.

Standard Operations

The instrument is provided with 2 USB interfaces, which are located on the back of the instrument (see Figure 2 on page 13). These interfaces can be used for exporting data and graphics to a printer, updating data and for data communication to a personal computer. These interfaces can be used for the connection of a USB stick, an external USB keyboard or a USB Barcode handset scanner.

Note: A USB hub may be used to connect several accessories at a time.

A USB memory stick is used to upgrade data and software, see

section 6.7.2 on page 98.

Important Note: A screened USB cable must not be longer than 3m!

Table 3 USB connector

5.2.6.1 Printer setup

1. Press PC & Printer in the Instrument Setup.

A list with information about the connections opens.

For reasons of compatibility, the printer language must be

HP PCL 3.

2. Press Printer.

3. Press Setup to display the Printer Setup screen.

Standard Operations

Printer Setup:

• Resolution: Print quality

• Paper: Paper size

Note: If an optional Thermal Printer is connected, the function "Auto Send" on/off is available.

4. Select Auto-Send: On to send all measured data automatically to the Thermal printer.

Note: The option Auto-Send is not available for any other printer (e.g. ink jet printer).

5. Press Resolution to select the print quality.

Select between

• 100 dpi

• 150 dpi and

• 300 dpi

6. Press OK to confirm.

Note: Press OK again to return to the PC & Printer menu.

5.2.6.2 Print data

Standard Operations

7. Press Paper to select the paper size.

Select between

• Letter

• Legal

• Executive

• A4

8. Press OK to confirm.

Note: Press OK again to return to the PC & Printer menu.

1. Press Recall Data in the Main Menu.

2. Select the data source, where the data to be printed are stored.

5.2.6.3 HACH Data Trans

3. A list is displayed. Data can be filtered. For more information

see section 5.3.1.2 on page 37.

4. Press the PC & Printer icon to send the data (table, curve) immediately to the printer.

5. Highlight Single point or Filtered data or All data and press OK to confirm.

Sending Data... is displayed until the data have been printed.

The optional HACH Data Trans software must be installed on the PC for the subsequent to process for measurement data.

1. Press PC & Printer in the Instrument Setup.

2. Select PC.

3. Press Setup to display the PC Setup screen.

For further installation instructions, refer to the HACH Data Trans user manual.

Standard Operations

5.2.7 Password

4. Select Auto-Send: On to send all measured data automatically

to the PC.

Note: If Auto-Send: Off is selected, the PC & Printer icon must be pressed, in order to send data to the PC.

Note: The remote function is only for monitoring the data transfer.

The Password menu contains a variety of security settings to control access to various functions. For example, prevent unauthorized changes to stored programs or instrument configurations.

1. Press Password in the Instrument Setup menu.

2. In order to highlight the Security List assign a password. Press Set Password.

3. Use the alphanumeric keypad to enter a new Password (up to

10 characters each) and press OK to confirm.

The access to the Security List is activated.

Standard Operations

4. Press Security List to lock various functions for unauthorized

users.

5. Highlight the desired functions to control.

6. Confirm the Security List with OK to return to the Password

menu.

7. Press On to highlight the new settings of the Security List.

5.2.7.1 Password deactivation

8. Enter the new Password again to confirm.

9. Press OK to return to Instrument Setup.

Note: The alphanumeric keypad to the Password inquiry appears when a user tries to reach a locked setting.

1. Press Password in the Instrument Setup.

2. Use the alphanumeric keypad to enter the former Password and press OK to confirm.

Standard Operations

3. Press Off to deactivate the settings of the Security List.

4. Press OK to return to Instrument Setup.

Note: Use this function to delete the former Password or to enter a new one.

5.3 Store, recall, send and delete data

5.3.1 The data log

The Data Log will store up to 500 readings taken in the modes: Stored Programs, Barcode Programs, User Programs, Favorite Programs, Single Wavelength and Multi Wavelength. A complete record of the analysis is stored, including the Date, Time, Results, Sample ID, and Operator ID.

5.3.1.1 Auto/manual data storage

The data storage parameter indicates whether data are to be stored automatically or manually (in which case the user has to decide which data to store).

1. Press Store: On/Off in the Options menu.

• With the Store On setting, all measurement data are stored

automatically.

• With the Store Off setting, no measurement data are

stored. However, this setting can be changed to Store On in the result display through configuration. The reading currently shown in the display is then stored.

Standard Operations

Note: When the instrument's memory (data log) is full, the oldest data are automatically deleted allowing the new data to be stored.

5.3.1.2 Recall stored data from the data log

1. Press Recall Data in the Main Menu.

2. Press Data Log.

3. Press Filter: On/Off.

A listing of the stored data is displayed.

Standard Operations

4. The function Filter Settings is used to search for specific

items.

5. Highlight On to turn on the filters to select data by

• Sample ID

• Operator ID

• Start Date

• Parameter

or any combination of the four.

6. Press OK to confirm the selection. The chosen items are listed.

7. Press View Details to get more information.

5.3.1.3 Send data from the data log

Data is sent from the data log as CSV (Comma Separated Value) files through a USB memory stick to a file named DATALOG. The file can then be processed using a spreadsheet program. The file name will be formatted as: DLYear_Month_Day_Hour_Minute_Second. CSV.

To send data to a Printer, see section 5.2.6.2 on page 33.

1. Plug in the USB device (Figure 2 on page 13).

2. Press Recall Data from the Main Menu. Press Options and then the PC & Printer icon.

3. Select the data to send to the memory stick and press OK.

Note: The number in parenthesis is the total number of data sets assigned to this selection.

To send measurement data to a PC:

The optional HACH Data Trans software must be installed on the PC, see section 5.2.6.3 on page 33.

5.3.1.4 Delete stored data from the data log

1. Press Recall Data in the Main Menu.

2. Press Data Log>Options>Delete.

3. Highlight Single Point or Filtered data or All data and press

Standard Operations

OK to confirm.

Note: The number in parentheses is the total number of data sets assigned to this selection.

Standard Operations

5.3.2 Time Course

5.3.2.1 Data Storage from Time Course

The instrument can store 20 Time Course Data sets. The data can be stored manually at the user's discretion after viewing the data.

1. Press the Store icon in the ”Options“ menu from Time Course mode after a reading is taken.

The Store Data list opens.

2. Press Store to save the current scan to the highlighted numbered line. A run can also be overwritten.

5.3.2.2 Recall Stored Data from Time Course

1. Press Recall Data in the ”Main Menu“.

a. Select Time Course to call up the required data.

b. If you are already working in this program, press Options,

More... and then Recall Data.

Standard Operations

2. Press Graph to look at details.

Note: Press View Summary to return to the Recall Data list.

3. Press Table to look at details.

Note: Press View Summary to return to the Recall Data list.

5.3.2.3 Send Data from Time Course

Option 1:

4. Press Recall Data in the ”Main Menu“ and then Time Course

to send the data to a USB memory stick or to a printer.

The files will be automatically send as CSV files (Comma Separated Value) to a file ”TCData“ (Time Course Data).

The file name will be formatted as: ”TCData_X.csv” X = number of scans (1–20)

For further processing use a spreadsheet program.

Standard Operations

Option 2:

5. Press Time Course and then Options, More... and Send Data

to send the data to a USB memory stick or to a printer.

The files will be automatically send as CSV files (Comma Separated Value) to a file ”TCData“ (Time Course Data).

The file name will be formatted as: ”TCYear_Month_Day_Hour_Minute_Second.CSV“ (Time Course Data)

For further processing use a spreadsheet program.

5.3.2.4 Delete Stored Data from Time Course

1. Press Recall Data from the ”Main Menu“ and then Time Course or ”Options“ menu, More..., Recall Data:

A listing of the stored data is displayed.

2. Highlight any data you want to delete.

3. Press Delete in the ”Options“ menu and confirm with OK.

5.4 Stored Programs

The instrument contains more than 200 programmed procedures. They can be accessed through the Stored Programs menu.

5.4.1 Select a saved test/method; enter user-specific basic data

1. Press Stored Programs in the Main Menu to view an

alphabetical list of stored programs with program numbers.

The Stored Programs list will appear.

2. Highlight the required test.

Note: Select the program number by name or use the arrow keys to scroll through the list quickly and highlight the program or press Select by number to search for a specific program number. Use the alphanumeric keypad to enter the test number and press OK.

3. Press Start to run the program. After a program is selected, the screen for that parameter will appear.

Standard Operations

5.4.2 Stored program options

Note: All corresponding data (wavelength, factors and constants) are already preset.

4. Follow the chemical procedures described in the corresponding Procedures Manual.

1. From the Main Menu, select Stored Programs. Select the necessary method and press Start.

2. Press Options for Parameter Setup. Refer to Table 4 for stored program descriptions.

Table 4 Stored programs options

Options Description

More For further Options

Standard Operations

Table 4 Stored programs options (continued)

Options Description

Store Off/On

% Trans/Conc/Abs To switch to % transmittance, concentration or absorbance readings

With the Store On setting, all measurement data are stored automatically. With the Store Off setting, no measurement data are stored.

Send Data icon / Send Data

Timer icon

Dilution Factor Off/On

Standard Addition

Standard Adjust

Chemical Form Some of the stored tests/methods allow to select the chemical form and the measuring range.

Reagent Blank

Save as User Program To store the selected parameters as a User Program, see section 6.1 on page 61.

Recall Data Call up saved measurement data or time courses, see section 5.3 on page 37.

To send Data to a printer, computer or USB memory stick (Type A)

This functions as a stopwatch. It helps to ensure that the steps of an analysis are correctly timed (e.g. reaction times, wait times, etc., can be exactly specified). When the specified time has elapsed, an acoustic signal is emitted. The use of the timer has no influence on the measurement program.

A corrective dilution factor can be entered in order to take account of certain properties. The number entered at the dilution factor prompt will be multiplied by the result to compensate for

the adjustment. For example, if the sample has been diluted by a factor of 2, enter 2. The default setting of the dilution factor is turned off.

Note: When a dilution is in effect, the dilution icon will appear on the display.

This enables the accuracy of the measurements to be checked. The (working) procedure for a test parameter contains a detailed explanation of how to use this function.

The (working) procedure for a test parameter indicates whether a standard adjustment is necessary and, if so, how to proceed.

Some of the stored tests/methods include the "Reagent Blank" function. This enables the reagent blank value to be added to or subtracted from, the subsequent readings. The reagent blank value shifts the calibration curve along the y-axis, without changing the shape or gradient of the curve. The effect corresponds to a y-axis intercept of the calibration straight line. This is made clear by the following equation: Concentration = [(Conc. factor) * Abs] – (reagent blank value).

Instrument Setup Basic data of the instrument, see section 5.2 on page 27.

5.4.3 Use of program timers

Standard Operations

Some procedures do not require the use of timers. Other procedures require several timers. These timers are pre-programmed into each Stored Program, along with a description of the activity to be performed during the timed period.

1. Press the Timer icon on the display.

2. Press OK to start the first timer.

The timer will count down on the screen.

3. To start the next timed activity for the Stored Program, press the Timer icon and OK.

Note: Press Close to view the measurement screen while the timer is running. The time will be shown left side bottom instead of the date.

Note: Press Cancel to stop the timer midway through the countdown.

Note: The Timer will beep when the end of the running time is

reached.

A general purpose timer is also available in many programs. When the timer icon is visible, press the icon and select General Timer. A new screen will appear. Enter the length of the timed interval and press OK to start the timer. The timer will beep when the timed interval ends.

Standard Operations

5.4.4 Set the dilution factor

The Dilution Factor function is used to calculate the original concentration of a sample that has been diluted by a known ratio. For example, dilution factor can bring the analyzed concentration within the test range.

1. Press Options>More…>Dilution Factor.

The number entered at the dilution factor prompt will be multiplied by the result to compensate for the adjustment.

For example, if the sample has been diluted by a factor of 2, enter 2. The default setting of the dilution factor is turned off.

2. Press OK to confirm. Press again OK.

3. Press Return to return to the result display.

4. Confirm ’Store present reading again?’ to save the current

measured value if necessary.

Note: When a dilution is in effect, the dilution icon will appear on the display.

Note: If using undiluted samples, set the dilution factor off again.

5.4.5 Run a standard adjust

The Standard Adjust function allows the calibration curve for a stored program to be adjusted based on analysis of a known standard solution. The Accuracy Check section of written procedures often suggests a standard solution concentration for this purpose.

Standard Operations

Read a standard before setting Standard Adjust to On.

1. Follow the entire procedure, using a known standard for the sample.

2. After reading the concentration, press Options>More>Standard Adjust.

3. If Standard Adjust is set to Off, turn it On.

The Current Reading will show the concentration. The box on the right will show the default standard value for the test, as mentioned in the procedure.

4. If the measurement used a standard concentration that is different from the one displayed in the box, press the box on the right to enter a different standard value and enter the new value. Press OK to confirm.

5.4.6 Set the chemical form

5. Press Adjust to enable the Standard Adjust. The Standard

Adjust icon will appear.

Note: The adjustment must be within certain limits, which vary with each program. The allowable percentage is shown after "Adjustment“.

Note: When a Standard Adjust is in effect, the Standard Adjust icon will appear on the display.

Some Stored Programs allow a variety of chemical forms to be selected.

Press the unit (e.g. mg/L) or the chemical representation of the evaluation form (e.g. Al

). A list of available evaluation forms is displayed. Select the required form by pressing the corresponding entry in the list.

Note: When the program is exited, the evaluation form reverts to the standard setting.

An alternative way of changing the standard setting:

Standard Operations

1. Press Options>More>Chemical Form.

2. Select the Chemical Form.

Note: The stoichiometric conversion of the measurement result is carried out automatically.

Note: The selected Chemical Form will appear on the display. Test results will be calculated and displayed in this chemical form.

5.4.6.1 Change of the default setting of the chemical form

1. Insert the sample cuvette or blank (depending on the working

procedure) into the cell compartment.

2. In the result display, press Options>More>Chemical Form.

3. A list of available evaluation forms appears. Select the new

default setting.

5.4.7 Run a reagent blank

4. Press Save as Default.

The current result and all further measurements will be displayed in the new chemical form.

Some of the stored tests/methods include the "Reagent Blank" function. This enables the reagent blank value to be measured and then taken into account in calculating the measurement result.

Measurement/analysis of a reagent blank:

1. Prepare the test/method in accordance with the (working)

procedure. Instead of a sample, deionized water is used to determine the reagent blank value.

2. Select the test. If required by the (working) procedure, insert the cell with deionized water into the cell compartment. Press

Zero.

3. Insert the prepared sample cell into the cell compartment. Press Read. The result is displayed.

Standard Operations

4. Press Options>More>Reagent Blank.

5. Press On to highlight the Reagent Blank function.

6. The concentration shown on the key is the measured value of

the reagent blank. To use this value for more analyses of this parameter, press OK.

7. To enter a previously recorded reagent blank value, press the key and use the alphanumeric keypad.

8. Press OK.

Note: The Reagent Blank function is deactivated when the measurement program is left. To use the same blank value later for other tests using the same reagent lot, enter the value per step 7.

Note: The results calculated using the reagent blank value must lie within the limits of the measuring range of the test/method.

5.4.8 Analysis of samples

Note: The reagent blank icon is shown in the result display (see arrow) when the function is active.

1. Press Stored Programs and select a program.

2. Insert the blank cuvette/cell into the cuvette/sample cell holder.

3. Press Zero.

Standard Operations

4. Remove zero solution and insert sample cuvette/cell into the

cell compartment.

5. Press Read. The result will be displayed.

6. For data storage, see section 5.3.1 on page 37.

5.4.9 Add stored programs to the favorite programs list

The Favorites menu simplifies test selection by creating a list of the most frequently used tests from the Stored Programs and User Programs.

1. Press Stored Programs in the Main Menu. The Stored Programs list will appear.

2. Highlight the selection by pressing it or Select by Number to search for the program by number.

3. Press Add to Favorites and press OK to confirm.

The program can now be selected from Favorite Programs menu in the Main Menu.

5.5 Barcode Programs

Standard Operations

A special barcode reader in cell compartment #1 automatically reads the barcode on the 13 mm cuvette/vial as the cuvette/vial completes a single rotation. The instrument uses the barcode identification to automatically set the correct wavelength for the analysis and calculates the result immediately with the help of the stored factors.

In addition, measured values are recorded at 10 different positions during a rotation. A special outlier-elimination program is run and then the average of the measured values is calculated. Cuvette/vial errors and soiling are recognized and highly precise results are obtained.

Figure 11 Cell compartment #1 for barcode 13 mm cuvettes/vials

5.5.1 Complete a barcode 13 mm test/vial

1. Insert the light shield in cell compartment #2.

2. Prepare the barcode 13 mm cuvette/vial in accordance with the

working procedure and insert the cuvette/vial in cell compartment #1.

• When a coded cuvette/vial is placed in cell compartment #1 (Figure 11), the corresponding measurement program is automatically activated in the Main Menu.

Standard Operations

5.5.2 Select the measuring range

• Otherwise, press Barcode Programs in the Main Menu.

and insert the blank or sample cuvette/vial (depending on the working procedure) in cell compartment #1.

The measurement is started automatically and the results are displayed.

To evaluate other cuvette/vial tests and other parameters, insert the prepared cuvette/vial into the cell compartment and read the result.

Some tests can be used for different measuring ranges. After the sample cuvette/vial has been inserted, a list of the different measuring ranges is displayed.

Select the required measuring range by highlighting the appropriate line.

Press Start Permanent if this measuring range is to apply to all subsequent measurements.

Changing the standard setting

In the result display, press Options>More>Permanent: On. The key will change to Permanent: Off.

5.5.3 Select the chemical evaluation form

The chemical form of the test result of a number of parameters can be selected individually.

In the result display, press the unit (e.g. mg/L) or the chemical representation of the evaluation form (e.g. PO possible evaluation forms is displayed, from which the required form can be selected. Press OK to confirm.

Another way of changing the standard setting is:

1. In the result display, press Options>More>Chemical Form.

A list of available evaluation forms appears.

2. Select the required chemical form and press OK to confirm.

–

–P). A list of

Note: The selected chemical form is displayed, but does not become the default. To change the default, see section 5.5.3.1.

5.5.3.1 Change of the default setting of the chemical form

1. Insert the blank or sample cuvette/cell (depending on the

working procedure) into the cell compartment.

2. In the result display, press Options>More>Chemical Form.

3. A list of available evaluation forms appear. Select the new

default setting.

4. Press Save as Default.

The current result and all further measurements will be displayed in the new chemical form.

5.5.4 Basic test-specific and sample-specific data settings

Press Options to change test or sample specific settings.

Standard Operations

Table 5 Barcode program options

Options Description

More For further Options

Store Off/On

Abs % Trans To switch to % transmittance or absorbance readings

Send Data icon / Send Data

Timer icon

Dilution Factor Off/On

Chemical Form Some of the stored tests/methods allow to select the chemical form and the measuring range.

Edit To modify an existing program

With the Store On setting, all measurement data are stored automatically. With the Store Off setting, no measurement data are stored.

To send data to a printer, computer or USB memory stick (Type A)

A corrective dilution factor can be entered in order to take account of certain properties. The number entered at the dilution factor prompt will be multiplied by the result to compensate for

the adjustment. For example, if the sample has been diluted by a factor of 2, enter 2. The default setting of the dilution factor is turned off.

Note: When a dilution is in effect, the dilution icon will appear on the display. Note: If undiluted samples are used, set the dilution factor off.

Standard Operations

Table 5 Barcode program options (continued)

Options Description

Save as User Program To store the selected parameters as a User Program, see section 6.1 on page 61.

Recall Data Call up saved measurement data or time courses, see section 5.3 on page 37.

Instrument Setup Basic data of the instrument, see section 5.2 on page 27.

5.5.5 Sample blank

Turbidity and color in the sample matrix can falsify the results of a photometric analysis. The interference factors come from the sample or are created by reactions with the reagents.

The influence of turbidity and/or color can be eliminated or reduced by taking a sample blank reading.

In the barcode mode, a special cuvette/vial (TNT919) containing the sample blank is placed in cell compartment #1 after the sample reading has been taken and is automatically measured. The sample reading is then corrected by adding or subtracting the blank value. The final result is displayed, with the message "After blank value corr.".

Some barcode tests do not require a sample blank value to be determined, as turbidity and color are dealt with during the test procedure. Other barcode tests use the reagent test vials to determine the sample blank. Please refer to the Hach procedure for method-specific instructions.

5.5.6 Update/edit barcode tests

5.5.6.1 Manual update of a barcode test

Using the data provided in the barcode, the instrument automatically sets the measurement wavelength and factors. If a discrepancy is detected between the barcode data and the stored data or a new test is identified, the instrument requests an update.

Data types are described in Table 6.

Table 6 Program update options

Program point Description

Name Name of the analysis parameter

Standard Operations

Version An abbreviation assigned by the user or the version number, etc. is entered here

Exact definition of the test: the number of wavelengths at which measurements are carried out,

Measurement process

Formula Definition of the evaluation formulas with which the test result is calculated

Variables

Timer 1, Timer 2, Timer 3, Timer 4

the number of absorbance measurements needed, the keys to be used, any waiting periods between measurements, etc.

The number of variables shown in the display depends on the definition of the measurement process and the formulas. Input of the numerical values of the wavelengths, factors, constants, etc.

This function can be used to enter abbreviations and defined times for up to four timers. Highlight the appropriate line and press Edit.

The timers are activated or deactivated with the control boxes on the left of the display. In the next column, a selection can be made from a list of names that describe the corresponding work step. In the third column, the times for each active timer are entered.

Enter the Name

Check the working procedure in advance to determine whether this point needs to be changed.

1. Highlight the line containing Name and press Edit. Use the alphanumeric keypad to enter the name indicated in the working procedure.

2. Press OK to confirm.

Standard Operations

Enter the Version

1. Highlight the line containing Versi on and press Edit. Use the

alphanumeric keypad to enter the name indicated in the working procedure.

2. Press OK to confirm.

Enter the Measurement Process

For detailed information on entering the measurement process, see

section 6.1.2 on page 72.

Check the working procedure in advance to determine whether this point needs to be changed.

1. Highlight the Measurement Process line in the data overview and press Edit.

2. Press Edit, select the sequence to be edited and press Delete.

3. Press New and use the alphanumeric keypad to enter the

process indicated in the working procedure.

Enter the Formula, Concentration Units, Designation, Measuring Ranges

For detailed information on entering the Formula, see section 6.1.2

on page 72.

Check the working procedure in advance to determine whether this information needs to be changed.

1. Highlight the Formula line in the data overview and press Edit.

2. Select the formula, press Edit and use the alphanumeric

keypad to enter the data specified in the working procedure (for C1=, C2=, units, name, measuring range limits, etc.).

Standard Operations

Enter the Variables (Factors, Wavelength and Coversion Factors)

For detailed information on entering the Variables, see section 6.1.2

on page 72.

Check the working procedure in advance to determine whether this point needs to be changed.

1. Highlight the Vari ables line in the data overview and press Edit.

2. Select the variable, press Edit and use the alphanumeric

keypad to enter the data specified in the working procedure (for F1, F2, λ1, U1 etc.). Confirm each entry with OK.

Abbreviation of Variables:

F1: Factor 1

F2: Factor 2

λ1: Wavelength 1

U1: Conversion Factor 1 for the first chemical form

U2: Conversion Factor 2 for additional chemical forms etc.

Timer 1, Timer 2, Timer 3 and Timer 4:

Standard Operations

5.5.6.2 Update an existing barcode test

Note: Only in very few cases does the revision of a test require all test specifications to be updated.

Option 1: Manual update of test data

1. Insert the light shield in cell compartment #2.

2. The instrument needs to be at the Main Menu or press Barcode Programs in the Main Menu.

3. Insert the blank or sample cuvette/vial (depending on the

working procedure) in cell compartment #1.

4. After "Barcode controlnumber?", a data overview is displayed, including the specifications of the test that is to be revised.

Note: The working procedures of the corresponding test contains the new data (Wavelength, Factors, Measurement Ranges, Conversion Factor for chemical Form, etc.).

5. Highlight the corresponding line and press Edit.

6. When finished programming, press Store and then Cancel to

return to the User Programs menu.

7. Press Start to begin the test with the new data.

Option 2: Manual check/revision of test data

If an incorrect entry was made during the data update and is not recognized via the barcode of the cuvette/vial (e.g. an incorrect measuring range), another option is available for checking and if necessary revising, the test data.

1. Insert the light shield in cell compartment #2.

2. The instrument need to be at the Main Menu or press Barcode Programs in the Main Menu.

3. Insert the blank or sample cuvette/vial (depending on the

working procedure) in cell compartment #1.

4. Press Options>More>Edit.

5. A data overview is displayed, including the specifications of the

test that is to be revised. Compare the displayed data with the data in the working procedure and edit the displayed data in accordance with the working procedure.

5.5.6.3 Program a new test

Standard Operations

1. The instrument needs to be at the Main Menu or press Barcode Programs in the Main Menu.

2. Insert the sample cuvette/vial or blank (depending on the

working procedure) into the cell compartment.

3. After "Program not available", a data overview is displayed, including the specifications of the test that is to be revised.

The working procedures of the corresponding test contains the new data (Wavelength, Factors, Measurement Ranges, Conversion Factor for chemical Form etc.).

4. Highlight the corresponding line including the specifications of the test that is to be programmed and press Edit.

5. To finish programming, press Store and then Cancel to return to the Main Menu.

6. Press Start to begin the test with the new data.

5.5.7 Upgrade of the instrument software

To obtain the software for the update from the Internet at

www.hach.com:

1. Go to http://www.hach.com.

2. On the DR 2800 product page, click Lab System Software/Software Update Downloads under Downloads.

3. Locate the appropriate download and follow the prompts for

saving the file(s) to the USB memory stick.

4. On the DR 2800, press Instrument Update in the System Checks menu.

5. Connect the USB stick to the USB interface on the DR 2800 (section 3.4 on page 13). Press OK.The link is established automatically and the software is updated.

Standard Operations

6. Press OK to return to the System Checks menu.

Note: When the instrument software has been updated, a prompt to restart the instrument is displayed.

Section 6 Advanced Operations

6.1 User Programs

User programs provide the opportunity to complete "made to measure" analysis.

The User Programs database is empty when the instrument leaves the factory and is used to accommodate programs created by users specific needs. Here are a few examples of entries:

• Programming of user-created procedures. The analysis procedure must be developed first, before it can be programmed. The user must define or determine the program sequences, calculation formulas, measurement wavelengths, factors, measuring range limits, etc.

• Modified tests

• Assignment of user programs to the favorites menu for

frequently used tests.

• Creation of a specific selection of methods and tests.

Press User Programs in the Main Menu and then Program Options. The Program Options menu contains several input and editing options (Table 7):

Table 7 Program options user programs

Options Description

Select New to program a new user program.

New

Add to favorites Select Add to favorites to add an existing user program to the list of frequently used programs.

Edit Select Edit to modify an existing program

Delete

Note: The first time Program Options is selected, only the New option is available. The other options remain inactive (grey) until the first program has been created.

Select Delete to remove a program from the list of user programs. The program will be simultaneously deleted from the Favorites list.

6.1.1 Program a user method

All input steps and their significance and options are explained in the following sections.

1. Select New in the Program Options menu.

Advanced Operations

Program Number:

Specific test number, with which the program can subsequently be called up from the selection list in the User Programs menu or the

Favorites menu.

2. Use the alphanumeric keypad to enter a program number

between 950 and 999. The lowest available number appears automatically.

3. Press OK.

Note: If the program number is already assigned to another user program, a message appears, asking whether the existing program should be replaced. Press OK to overwrite the existing program.

Program Name:

4. Use the alphanumeric keypad to enter a program name. The

name can be a maximum of 28 characters long.

Program Type Description

5. Press Back to go back to the previous program point or press Next to continue with the input of the program data.

Program Type:

6. Select the required option (Table 8) and press Next.

7. If the Single Wavelength (section 6.1.1.1 on page 63) or Multi

Wavelength (section 6.1.1.2 on page 64) is selected, define the unit, wavelength, absorbance formula, wavelength λx, concentration factor Kx, concentration resolution, chemical form and calibration equation. More information on Free Programming parameters see

section 6.1.2 on page 72.

Table 8 Program descriptions

Single Wavelength Measurements at a defined wavelength

Multi Wavelength

Free Programming

In the Multi Wavelength mode, absorbance values can be measured at up to four wavelengths and the results can be mathematically processed to obtain sums, differences and relationships.

This is an advanced form of programming for original user-developed methods. In Free Programming, the user defines the measurement process, variables and calculations involved in obtaining a reading.

6.1.1.1 Single wavelength settings

Advanced Operations

If the Single Wavelength mode is selected, the following parameters can be defined:

Units: Select the required unit from the list and press Next.

Note: Units of measure not included in this list can be added in the edit program under Program Options, Edit. Select Units, Edit and then New.

Wavelength (single wavelength program type):

Use the alphanumeric keypad to enter the measurement wavelength. The entered wavelength must be in the range from 340–900 nm.

Press Next to proceed.

Concentration resolution (number of decimal places)

Select the required number of decimal places from the displayed list and press Next.

Advanced Operations

6.1.1.2 Multi wavelength settings

Chemical form:

Enter the chemical formula used in the display to represent the analysis parameter.

Use the alphanumeric keypad to enter the chemical form and press Next to enter Calibration settings.

If the Multi Wavelength mode is selected, the following parameters can be defined:

Units: Select the required unit from the list and press Next.

Note: Units of measure not included in this list can be added in the edit program under Program Options, Edit. Select Units, Edit and then New.

Absorbance formula (multi-wavelength program type):

The Absorbance Formula menu is used to define the wavelengths and the coefficients used in the formula. The absorbance formula defines the calculation for the multi-wavelength measurement. Press the appropriate key to edit the input.

Press the Formula key.

In the displayed list, select the formula for the program and press

OK to confirm.

Advanced Operations

List of available absorbance formulas

is the absorbance at wavelength 1,

is the absorbance at wavelength 2 and so on

is the factor at wavelength 1,

is the factor at wavelength 2 and so on

If a subtraction has to be completed, the factors can be entered with a minus sign.

Wavelength λ

Press a λ wavelength. Press another λ

key and use the alphanumeric keypad to enter a

key and enter the next wavelength. If

necessary, repeat until all the wavelengths for the formula have been entered. The wavelengths must be in the range from 340–900 nm. Press OK.

Concentration Factor Kx

Multiplication factor for converting absorbance values into concentration values.

Press a factor key and use the alphanumeric keypad to enter a factor. If the formula includes more than one factor, press another factor key and enter another factor and repeat until all the factors have been entered. Press OK. When all the relevant data have been entered, press Next.

Note: Up to 5 digits can be entered, including a maximum of 4 decimal places to the right of the decimal.

Concentration resolution (number of decimal places)

Select the required number of decimal places from the displayed list and press Next.

Advanced Operations

Chemical form:

Enter the chemical formula used in the display to represent the analysis parameter.

Use the alphanumeric keypad to enter the chemical form and press

Next to enter Calibration settings.

6.1.1.3 Calibration settings for single and multi wavelength mode

A method is calibrated by determining the absorbance values of several standard solutions of known concentration.

There are three ways to create and store a calibration curve. Instructions for each method follows (Table 9).

Table 9 Calibration settings

Mode Descriptions

A calibration table is created by entering the concentration values and the absorbance values of

Enter values

Read Standards

Enter Formula

the analyte solution. The absorbance values are plotted versus standard concentrations and the calibration curve is displayed as a graph (page 66).

A calibration table is created by entering the concentration values of the standard solutions and then measuring the absorbance of the analyte solutions. The absorbance values are plotted versus standard concentrations and the calibration curve is displayed as a graph (page 68).

If the calibration curve can be determined from the mathematical relationship between concentration and absorbance by linear regression, etc., the corresponding formula can be selected (linear, 2nd or 3rd order polynomial) from a list and the appropriate factors can be entered (page 69).

Calibration by entering calibration values

Enter concentration/absorbance

1. Select the Enter Values and press Next.

Advanced Operations

2. To enter the standard concentrations and corresponding

absorbance values in the displayed table, press the "+" symbol. Use the alphanumeric keypad to enter the values.

Press OK and enter the corresponding absorbance value. Press OK.

The entered data are displayed in the table. Repeat the sequence for each data point to enter it.

3. To change a value in the table, highlight the appropriate line, press the unit key (e.g. mg/L) or Abs and enter the changed value via the alphanumeric keypad.

4. When the data have all been entered, press Graph.

Note: The correlation coefficient (r2) is shown on the left below the axes.

5. The linear equation corresponds to the standard setting. Press Next Curve to display the polynomial 2nd order curve. Press Next Curve again, to display the polynomial 3rd order curve.

6. Press Force 0 to change the setting from Off to On. The curve

now passes through the origin of the coordinate system.

Note: This may have an adverse effect on the correlation coefficient

7. Press Table to display the table again.

8. When the table has been completed and the curve type has

been chosen, press Done when the graph is displayed or Exit when the table is displayed. Go to section 6.1.1.4 on page 70.

Advanced Operations

Calibration by reading standards

1. Press Read Standards and press Next.

2. To enter the standard concentrations in the displayed table,

press the "+" symbol. Use the alphanumeric keypad to enter the standard concentration. Press OK.

3. Press the "+" symbol again (see arrow) and enter the next standard concentration. Repeat this sequence until all standard conentrations (maximum of 24 solutions) have been entered.

4. Highlight the line with the appropriate concentration and insert the cuvette with the corresponding standard solution.

5. Insert the zero solution into the cell compartment. Press Zero.

6. Insert the first standard solution into the cell compartment.

Press Read.

Insert the second standard solution into the cell compartment. Press Read.

Repeat this sequence until all the standard solutions have been measured (maximum of 24 solutions).

The entered and measured data are displayed in the table.

Note: To delete a standard concentration, highlight the appropriate line and press the Delete icon.

The timer icon shown in the display helps to ensure, when necessary, that the steps of an analysis are correctly timed (e.g. reaction times, wait times, etc., can be exactly specified). When the specified time has elapsed, an acoustic signal is emitted. The use of the timer has no influence on the measurement program.

7. When the data have all been entered and the measurements have all been completed, press Graph.

8. The linear curve corresponds to the standard setting. Press Next Curve to display the polynomial 2nd order curve.Press Next Curve again to display the polynomial 3rd order curve.

Advanced Operations

9. Press Force 0 to change the setting from Off to On. The curve

then passes through the origin of the coordinate system.

Note: This may have an adverse effect on the correlation coefficient (r2).

10. Press Table to display the table again.

11. When the table has been completed and the curve type has

been chosen, press Done when the graph is displayed or Exit when the table is displayed. Go to section 6.1.1.4 on page 70.

Calibration by entering the formula

1. Press Enter Formula and press Next.

2. Press the formula key.

A list of available formulas (linear and 2nd and 3rd order polynomial) is displayed. Up to 4 coefficients can be entered, depending on the selected formula. Press the required formula.

3. Depending on the selected formula, the required coefficients (a, b, c...) are displayed. Press the coefficient keys and enter the corresponding values via the alphanumeric keypad. After each entry, press OK to confirm.

Note: The coefficients can have 5 digits and can have a positive or a negative sign.

Advanced Operations

6.1.1.4 Store a user program

The input of the basic data is complete. An overview of the variable program data is displayed.

1. To enter more specifications or change existing ones, highlight the appropriate line and press Edit.

2. Select Store to save the user program.

3. Press the PC & Printer icon to send the program data to a

printer or to a USB memory stick (connect the USB memory stick to the USB interface first).

Note: The test data will be formatted in the preinstalled PrgData folder as a .csv and as a .lst file.

4. To transfer the program data from one instrument to another create a new folder on the USB memory stick named dbhlc and copy the .lst files into this folder. (see section 6.7.2 on

page 98).

6.1.1.5 Additional user-defined parameters and functions

In addition to the previously defined basic data, additional parameters and functions can be defined for user programs:

• measuring range upper and lower limits

• timer functions

• chemical forms

Upper and lower limits of the measuring range

It is possible to enter a maximum (upper) and minimum (lower) measurement value. An error message is displayed if a reading is above the upper limit or below the lower limit.

1. Highlight the appropriate line in the overview of the program data and press Edit.

2. Set On and press the 0.000 key to enter the measuring range limit. Confirm the entry by pressing OK.

Advanced Operations

Timer 1 / Timer 2 / Timer 3 / Timer 4:

This function can define time intervals for up to four timers. Timer designations such as Shake, Wait and Swirl can be assigned.

1. Highlight the appropriate line in the overview of the program data and press Edit.

2. The timers are activated or deactivated with the check boxes in the left part of the display. In the next column, a selection can be made from a list of names that designate the corresponding work step. In the third column, the times for each timer are entered (in mm:ss).

Chemical form 2 / chemical form 3 / chemical form 4:

If a Chemical Form 1 has been defined, up to three additional alternative forms can be entered here.

1. Highlight the appropriate line in the overview of the program data and press Edit.

2. The chemical forms are activated or deactivated with the check boxes in the left part of the display.

3. Press the left key to enter another chemical form with the alphanumeric keypad and press OK to confirm. Press the right key to enter the conversion factor to calculate the concentration of the additional chemical form from the concentration of Chemical Form 1 and press OK to confirm.

4. Press Store to save the program data. Press Cancel to return to the Main Menu.

Advanced Operations

6.1.2 Free programming program type

Free Programming is an advanced option for entering original user-developed methods. When the Free Programming option is selected, an overview of the specifications of the programmed test is displayed. Each input option can be modified to develop the user method. Refer to Table 10 for more information. To modify an input option, select the appropriate line and press Edit.

Important Note: Perform steps 1–6 of Programming a New User Method (section 6.1.1 on page 61) before proceeding with Free Programming.

Highlight the line containing the program point that is to be edited or defined and press Edit.

Table 10 Definitions of the program points

Program point Description

Name Name of the analysis parameter

Version An abbreviation or version number assigned by the user is entered here.

Exact definition of the test: the number of wavelengths at which measurements are made, the

Measurement Process

Formula Definition of the formulas with which the test result is calculated.

Variables

Timer 1, Timer 2, Timer 3, Timer 4

number of absorbance measurements needed, the keys to be used, any waiting periods between measurements, etc.

The number of variables shown in the display depends on the definition of the measurement process and the formulas. Input of the numerical values of the wavelengths, factors, constants, etc.

Used to enter abbreviations and defined times for up to four timers. Highlight the appropriate line and press Edit. The timers are activated or deactivated with the control boxes on the left of the display. In the next column, a selection can be made from a list of names that describe the corresponding work step. In the third column, the times for each active timer are entered.

6.1.2.1 Measurement process

The measurement process defines the handling and the measurements of the test:

• At which and how many wavelengths should measurements be completed

• How many absorbance measurements must be completed?

• When should the zero measurement and the sample

measurement be completed?

• Are waiting times necessary between measurements?

• Should individual program sequences be repeated?

The elements of a measuring sequence, such as zero and sample measurements and the timer(s) (reaction times, waiting times, etc.) are individually defined.

6.1.2.2 Enter a new element of a measuring sequence

Important Note: Each component of the measurement process must be entered in the order in which it will be completed.

1. Highlight the Measurement Process line in the data overview and press Edit.

2. Press Edit again and then New.

Advanced Operations

Content and definition of the keys

[Z] key / Zeroing

1. Press the [Z] key to program a zero measurement. Confirm with OK.

2. Press New and then Zeroing... and use the alphanumeric

keypad to enter the wavelength at which the zero measurement is to be completed. Press OK and confirm the input by pressing

OK again.

3. If zero measurements are to be carried out at a number of

wavelengths, repeat the above two steps for each wavelength.

Note: The entered measurement sequence is displayed.

Advanced Operations

Process Timer key

1. Press the Process Timer key to enter any waiting, reaction or

handling times that have to be taken into account. Use the alphanumeric keypad to enter the time. Press OK and confirm the input by pressing OK again.

Note: This time is integrated into the measurement process.

Note: The entered measurement sequence is displayed.

[R] key / Reading...

1. Press the [R] key to program a measurement of the substance that is to be analyzed. Confirm with OK.

2. Press New and then Reading... and use the alphanumeric

keypad to enter the wavelength at which the measurement is to be completed. Press OK and confirm the input by pressing OK again.

3. If measurements are to be completed at a number of wavelengths, repeat the above two steps for each wavelength.

Note: The entered measurement sequence is displayed.

{ } key Elements of the measurement sequence that are to be repeated are placed in brackets.

The left bracket "{"marks the start of the sequence that is to be repeated and the right bracket "}" marks the end.

Note: The key showing the right bracket remains inactive until a left bracket is entered.

1. Press {.

2. Press the key that defines the sequence that is to be repeated: [Z] or [R]. Confirm with OK.

3. Press New and then press Zeroing... or Reading... and use

the alphanumeric keypad to enter the wavelength at which the measurement should be completed. Press OK and confirm the input by pressing OK again.

4. Press } to end the sequence.

Note: If an action such as a zero measurement recurs at different stages of a measurement sequence, the series of actions is numbered sequentially (e.g. Z1, Z2, etc.).

Deleting an element of a measuring sequence

Select the appropriate line and press Delete. The element is deleted.

Inserting an element of a measuring sequence

Select the line in the measuring sequence where the insertion is be made and press New.

A new element can be entered at the selected position.

When the input is complete, press OK in the "Measurement Process" display. The data overview is then displayed.

6.1.2.3 Enter the calibration formula (evaluation formula)

The calibration formula (evaluation formula) defines the calculation and display of intermediate and final results. The previously defined elements of the measuring sequence are the basis for calculating the concentrations.

Advanced Operations

Enter calibration formula C1

1. Highlight the Formula line in the data overview and press Edit.

2. Highlight the line C1: Off and press Edit.

3. Select C1: Off again and press Edit. The display changes to C1: On.

4. Highlight the next line C1 = to define the formula and press Edit.

Advanced Operations

Screen Key Description

Refer to Table 11 for detailed information on the Edit formula keys.

Note: The evaluation formula is built up successively in the display in accordance with the input.

Note: The arrow key deletes the most recently entered element of the formula.

Table 11 Edit formula key descriptions

Press the Abs/Variables key to select, from the displayed list, the required element of the defined

Abs/Variables

measuring sequence and therefore the corresponding measurement wavelength, so that this can be taken into account in the formula.

New Number Press New Number to enter a new factor or constant.

Table 11 Edit formula key descriptions (continued)

Screen Key Description

Press +– ÷x, to enter a mathematical operation. Select the operation and press OK to confirm. The available choice of mathematical operations

depends on the defined formula. This means that functions such as "( )" or "ln"/"log" etc.

are only active if a term in parentheses or the calculation of a logarithm is mathematically permissible in the defined formula (this also applies to the basic mathematical operations).

+–

÷x

The following mathematical operations are available:

• + (Addition)

• – (Subtraction)

• ÷ (Division)

• x (Multiplication)

• ^ (Exponent)

• Ln (Natural logarithm)

Advanced Operations

• Log (Common logarithm)

Press >=< to include logic statements/links/conditions in the formula. The following functions are available:

= (Equal to), < (Less than), > (Greater than), <= (Less than or equal to), >= (Greater than or equal to), IF, THEN, ELSE

>=<

When the evaluation formula C1 has been entered completely, press OK to confirm. Press OK again to return to the Formula display.

When the formula C1 has been entered and confirmed, the parameter name, upper and lower limits of the measuring range and display result (yes, no) can be entered.

Enter the next calibration formula (C2 or Cn)

1. Highlight C2: Off and press Edit.

2. Select C2: Off again and press Edit. The display switches to C2: On.

3. Highlight the next line C2 = to define the formula and press Edit.

In addition to the keys described in Table 11, only one other function is available:

Advanced Operations

6.1.2.4 Enter variables

Select Conc. key

If an already defined formula, in this case C1, is to be taken into account in the formula for C2, press Select conc.

Enter the number of the formula (e.g. 1 for C1) and press OK to confirm.

Cn can now be linked with a mathematical operation.

Note: The Cn concentrations that are to be calculated are numbered in sequence: C1, C2, C3, etc.

Note: When the first Cn formula has been defined, the Formula list is automatically extended by Cn+1.

1. Highlight the Variables line in the data overview and press Edit.

2. Select the variable to be edited, press Edit and use the

alphanumeric keypad to enter the data specified in the working procedure (for F1, F2, λ1, U1 etc.). Press OK to confirm each entry.

Abbreviation of Variables:

F1: Factor 1

F2: Factor 2

λ1: Wavelength 1

U1: Conversion Factor 1 for the first chemical form

U2: Conversion Factor 2 for the further chemical form etc.

6.1.2.5 Save a free programming user program

1. Press Store to save the entered data. The data can be stored

under any data point (Measurement sequence, Formula, Timer, etc.).

2. Press the PC & Printer icon to send the program data to a printer or to a USB memory stick (connect the USB memory stick to the USB interface first).

Note: The test data will be formatted in the preinstalled PrgData folder as a .csv and as a .lst file.

3. To transfer the program data from one instrument to another create a new folder on the USB memory stick named dbhlc and copy the .lst files into this folder (see section 6.7.2 on

page 98).

6.1.3 Select a user program

1. Press User Programs in the Main Menu to view an

alphabetical list of user programs with program numbers.

The User Programs list will appear.

Note: Use the scroll bar to scroll through the list quickly.

Note: Highlight the selection by pressing it or press Select by Number to search for the program by number.

2. Press Start to run the program.

6.1.4 Add, edit and delete user programs from the favorites list

The most frequently used tests/methods in the User Program menu can also be added to the list of favorites to simplify their selection.

Advanced Operations

1. Press User Programs in the Main Menu.

The User Programs list will appear.

2. Highlight the selection by pressing it or press Select by Number to search for the program by number.

Note: Use the scroll bar to scroll through the list quickly. Use the alphanumeric keypad to enter the test number (program number) and press OK to confirm.

3. Press Program Options.

4. Press Add to Favorites, Edit or Delete and press OK to

confirm.

Note: If the stored program is deleted in User Programs, it will also be deleted in Favorites Programs.

Advanced Operations

6.1.4.1 Add to Favorites

6.1.4.2 Edit

1. Press Add to Favorites and press OK to confirm.

The program is added to the Favorites.

1. Press Edit and press OK to confirm.

6.1.4.3 Delete

An overview of the specifications of the programmed test is displayed. More information about the input options is provided in the section 6.1.2 on page 72.

1. Press Delete and press OK to confirm.

The program is deleted from the list of User Programs.

Note: If the stored program is deleted in User Programs, it will also be deleted in Favorites Programs.

6.2 Favorite Programs

6.2.1 Recall a favorite program

Advanced Operations

The most frequently used tests/methods in the Stored Programs menu and the User Programs menu can also be added to the list of favorites to simplify their selection.

To add Stored Programs and/or User Programs to the favorites list or the favorite programs, see section 6.1.4 on page 79.

1. Press Favorite Programs in the Main Menu.

The Favorite Programs list will appear.

2. Highlight the selection by pressing it.

Note: Use the scroll bar to scroll through the list quickly.

Note: Press Select by Number to search for the program by number.

Use the alphanumeric keypad to enter the test number (program number) and confirm the input by pressing OK.

6.2.2 Delete a favorite program

3. Press Start.

1. Press Favorite Programs in the Main Menu.

The Favorite Programs list will appear.

2. Highlight the selection by pressing it.

Note: Use the scroll bar to scroll through the list quickly.

Note: Press Select by Number to search for the program by number.

Use the alphanumeric keypad to enter the test number (program number) and confirm the input by pressing OK.

3. Press Remove Program and press OK to confirm.

Note: If a Favorite Programs is deleted, it will stay in the User Programs or Stored Programs.

Note: If the stored program is deleted in User Programs, it will also be

deleted in Favorites Programs.

Advanced Operations

6.3 Standard Addition – monitoring/checking results

The accuracy of measured values (their correspondence with the actual concentration of the analyte in the sample) and their precision (correspondence of the measurement results obtained from several samples containing the same concentration of the test analyte) can be determined or improved using the standard addition method.

This method (also referred to as spiking) serves to identify sample-specific interference factors, e.g. substances in the sample that falsify the analysis (sample matrix effect), a defective measuring instrument or contaminated reagents.

Method:

A defined amount (concentration) of a standard solution of the test substance is added to the sample. The detection rate should be close to 100%.

Measured value after a standard addition

Detection rate

------------------------------------------------------------------------------------------------------------ -

Expected value after a standard addition

Detection rate Conclusion

100%

< 100%

Probability that the measurement results are correct is high.

Assumption: The analysis was falsified by substances in the sample (sample matrix effect)

Test to determine whether a sample matrix effect is present:

Use distilled water instead of the sample. Add standard solution as described in the procedure.

Detection rate Conclusion

Ions in the sample are

100%

interfering with the analysis, causing false results to be obtained.

No interfering ions -

≠ 100%

consider other interference factors

Measures to identify other interference factors:

Checklist:

1. Check if the procedure is completed correctly:

a. Are the reagents added in the correct order?

b. Is enough time allowed for color development?

c. Is the correct glassware in use?

d. Is the glassware clean?

Advanced Operations

e. Does the test require the sample to be at a certain

temperature?

f. Was the pH of the sample in the correct range?

g. Is the pipette volume correct?

2. Check the used reagents by repeating the standard addition

procedure with freshly prepared reagents.

Detection rate Conclusion

The originally used reagents were defective.

100%

If none of these measures resolves the problem, please contact the manufacturer or a sales representative.

Check the standard solution:

Repeat the standard addition procedure with a freshly prepared standard solution.

Detection rate Conclusion

The originally used

100%

standard solution was defective.

6.3.1 Complete a standard addition

Carry out the standard addition in accordance with the corresponding procedure.

There are two different methods:

Peak volume (Standard addition):

Defined volumes of a standard solution are added step by step to an already analyzed sample. The sample is measured again after each addition.

Sample volume:

A defined volume of a standard solution of known concentration is added to the volume of sample specified in the procedure and the sample is measured after each addition. In most cases three different standard solutions are prepared and the procedure is repeated for each of them.

Note: The units and chemical forms used for the sample are used for the standard solutions. Be careful to ensure that to use the correct units for subsequent entries.

Advanced Operations

Peak volume/sample volume methods

1. Select Stored Programs in the main menu. Select the required

program.

2. Press Start.

3. Analyze a sample without added standard solution in

accordance with the instructions in the Procedures Manual. When the measurement is complete, leave the sample cuvette/cell in the cuvette/sample cell holder.

4. Press Options>More>Standard Addition.

An overview of the data of the standard addition procedure is displayed.

5. Press OK to accept the standard values for standard concentration, sample volume (total) and standard addition volume. Press Edit to change any of these values.

Advanced Operations

6. Press the key of the value to change it. Use the alphanumeric keypad to change the value. Press OK to confirm.

7. Press the keys to enter the standard addition volumes. Use the alphanumeric keypad to enter the new data and press OK.

Description of the table of readings

• The first column shows the standard addition volume. 0 mL

stands for a sample containing no added standard.

• The second column shows the reading of the samples with and without added standard.

• The third column shows the detection rate of the added standard.

Note: The black highlighted line is active.

8. The reading of the sample in the instrument, without added standard, is automatically shown under 0 mL.

9. Prepare the standard addition solution as described in the procedure.

10. Use the arrow keys to select the first standard addition volume in the table and insert the cuvette with the corresponding volume of added standard into the cell compartment. Press Read.

Repeat the procedure from point 8 with all the other standard addition solutions.

Advanced Operations

11. After all the standard addition solutions have been measured,

press Graph.

The regression line through the standard addition data points is displayed.

The correlation coefficient r

indicates how close the data

points are to the line.

If the correlation coefficient = 1, the curve is linear.

The concentration shown above the curve is the estimated concentration of the sample without the added standard.

Note: In the curve menu, the name on the Curve key switches to Table. Press Table to display all the data in the table again.

12. Press Ideal line to display the relationship between the added standard solutions and the ideal line (detection rate 100%).

Advanced Operations

6.4 Single Wavelength (absorbance, concentration and transmittance measurements)

The Single Wavelength mode can be used in three ways. For sample measurements at a single wavelength, the instrument can be programmed to measure the absorbance, % transmittance or concentration of the analyte.

Absorbance measures the amount of light absorbed by the sample, in units of absorbance.

% transmittance measures the percent of the original light that passes through the sample and reaches the detector.

Turn the concentration factor on to select a specific multiplier for converting absorbance readings to concentration. In a graph of concentration versus the absorbance, the concentration factor is the slope of the line.

6.4.1 Set up single wavelength mode

Press Single Wavelength in the Main Menu. Press Options for Parameter Setup.

Table 12 Single wavelength setup options

Options Description

More For further Options

Store Off/On

% Trans/Abs To switch to % transmittance, concentration or absorbance readings

λ Wavelength

Timer icon

Concentration Factor Multiplication factor for converting absorbance values into concentration values.

Concentration Resolution

Save as User Program To store the selected parameters as a User Program, see section 6.1 on page 61

Recall Data Call up saved measurement data or time courses, see section 5.3 on page 37.

With the Store On setting, all measurement data are stored automatically. With the Store Off setting, no measurement data are stored.

To enter the measurement wavelength. Use the alphanumeric keypad to enter the measurement wavelength. The entered wavelength must be in the range from 340–900 nm.

To select the position of the decimal point in the calculated concentration readings.

Advanced Operations

Table 12 Single wavelength setup options (continued)

Options Description

Instrument Setup Basic data of the instrument, see section 5.2 on page 27.

Concentration factor:

1. Press Concentration Factor: Off in the Options menu. Press On to highlight this feature.

2. Press the ”Factor“ key and use the alphanumeric keypad to

enter the factor by which absorbance readings are to be multiplied. Press the ”Unit“ key to select the units for concentration measurements or to create a new unit.

3. Press OK to confirm.

Concentration resolution:

1. Press Concentration Resolution in the Options menu.

2. Select the resolution and press OK to confirm.

6.4.2 Take single wavelength measurements

1. Insert the blank cuvette/cell into the cuvette/sample cell holder. Press Zero.

Note: The Read key is only active after the zero measurement has been completed.

2. Insert the sample cuvette/cell into the cuvette/sample cell holder. Press Read.

3. For data storage, see section 5.3.1 on page 37.

Advanced Operations

6.5 Multi-Wavelength mode – measurements at more than one wavelength

In the multi-wavelength mode, absorbance values can be measured at up to four wavelengths and the results can be mathematically processed to obtain sums, differences and relationships.

Absorbance measures the amount of light absorbed by the sample, in units of absorbance.

% Transmittance measures the percent of the original light that passes through the sample and reaches the detector.

Turning on the concentration factor allows selection of a specific multiplier for converting absorbance readings to concentration. In a graph of concentration versus the absorbance, the concentration factor is the slope of the line. Concentration is calculated using a single factor for each wavelength, which is input by the user.

6.5.1 Set the reading mode at different wavelengths

Press Multi Wavelength in the Main Menu. Press Options for Parameter Setup.

Table 13 Multi-wavelength setup options

Options Description

More For further Options

Store Off/On

% Trans/Abs To switch to % transmittance, concentration or absorbance readings

λ Wavelength

Timer icon

Concentration Factor Multiplication factor for converting absorbance values into concentration values.

Concentration Resolution

Absorbance Formula Calculation basis for evaluating samples

Save as User Program To store the selected parameters as a User Program, see section 6.1 on page 61

With the Store On setting, all measurement data are stored automatically. With the Store Off setting, no measurement data are stored.

To enter the measurement wavelength. Use the alphanumeric keypad to enter the measurement wavelength. The entered wavelength must be in the range from 340–900 nm.

To select the position of the decimal point in the calculated concentration readings.

Advanced Operations

Table 13 Multi-wavelength setup options (continued)

Options Description

Recall Data Call up saved measurement data or time courses, see section 5.3 on page 37.

Instrument Setup Basic data of the instrument, see section 5.2 on page 27.

λ / Absorbance formula:

1. Press Absorbance Formula.

2. The formula selected in the top key determines the number of

wavelength and coefficent keys that will appear below. To change the absorbance formula, press the top key, select a formula from the displayed list and press OK. When a new formula is selected, the number of variables below changes to match.

The following formulas are available:

A 1 + K 2 A

K 1 A 1 + K 2 A 2 + K 3 A K 1 A 1 + K 2 A 2 + K 3 A 3 + K 4 A K 1 A 1 / K 2 A (K 1 A 1 + K 2 A 2 ) / K 3 A

(K 1 A 1 + K 2 A 2 ) / (K 3 A 3 + K 4 A 4 )

refers to the absorbance at wavelength 1

refers to the absorbance at wavelength 2, etc.

refers to the coefficient at wavelength 1

refers to the coefficient at wavelength 2, etc.

Coefficients can be set negative where subtraction is required.

Advanced Operations

3. To change a wavelength, press one of the ”λx:“ keys. Enter the

desired wavelength coefficient into the numeric keypad. Press OK to confirm.

4. To change a coefficient, press one of the ”K

:“ keys. Enter the

desired coefficient into the numeric keypad. Press OK to confirm.

Note: The instrument allows entry of up to 5 significant digits, with a maximum of 4 significant digits after the decimal point.

Concentration factor:

1. Press Concentration Factor: Off in the Options menu. Press On to highlight this feature.

2. Press the ”Factor“ key to enter the factor by which

absorbance readings are to be multiplied. Press the ”Unit“ key to select the units for concentration measurements or to create a new unit.

3. Press OK to confirm.

Concentration resolution:

1. Press Concentration Resolution in the Options menu.

2. Select the resolution and press OK to confirm.

6.5.2 Complete a measurement in the multi wavelength mode

1. Insert the blank cuvette/cell into the cuvette/sample cell holder. Press Zero.

Note: The Read key does not become active until the zero measurement has been completed.

2. Insert the sample cuvette/cell into the cuvette/sample cell holder. Press Read.

3. For data storage, see section 5.3.1 on page 37.

Advanced Operations

6.6 Time course of absorbance/transmittance

The Time Course Mode is used to collect data in either absorbance or transmittance for a user-specified length of time. After the data are collected, they can be displayed in either graphic or tabular format.

6.6.1 Time course setup parameters

Press Time Course mode in the Main Menu. Press Options to configure parameters.

Table 14 Time course setup options

Option Description

More For further Options

Store icon To store the scan data

Time & Interval

To input the total time for data collection and the time interval between the collection of data points

λ To input the wavelength setting

View Table

Scale & Units

Send Data To send Data to a printer, computer or USB memory stick (Type A)

Recall Data Call up saved measurement data or time courses, see section 5.3 on page 37.

Instrument Setup Basic data of the instrument, see section 5.2 on page 27.

To display readings in absorbance, transmittance or concentration. This can be changed after sample data are collected.

Scale: In the automatic scaling mode, the y-axis is automatically adjusted so that the total scan is displayed. The manual scaling mode allows sections of the scan to be displayed.

Units: Choice of absorbance or transmittance.

Advanced Operations

Time & interval:

1. Press Time & Interval in the Options menu.

2. Input the total time and the reading time and press OK to

confirm.

Note: In total 500 measuring steps are possible. To select a total time and a time interval that would cause this number of measurements to be exceeded, the time interval is defined automatically and the OK key is inactivated.

Scale & units:

1. Press Scale & Units in the Options menu.

2. Highlight Abs or %T as the required units.

6.6.2 Time course scan reading

3. Highlight Auto or Manual scaling on the graph´s y-axis.

Note: If manual scaling is selected, use the alphanumeric keypad to set the

min.

and y

limits y selected range. If automatic scaling is selected, the instrument sets the limits automatically so that the total range can be displayed.

. The graph is adjusted to display only the values in the

max.

4. Press OK to confirm.

5. Press Return to return to the scan mode.

After the parameters have been selected, the instrument must be blanked, then the sample can be analyzed.

1. Insert the blank cuvette/cell into the cuvette/sample cell holder. Press Zero. The blank reading is shown on the display.

2. Insert the sample cuvette/cell into the cuvette/sample cell holder. Press Read. Start collecting time course (kinetic) data.

Note: During the measurement the Zero and Read keys change to Mark and Stop.

Advanced Operations

6.6.3 Analysis of time course data

• Select Mark to mark the next data point collected. This mark is

not used by the instrument, but is available for the user and may indicate a significant event, such as the addition of a sample or other reagent. The mark is also shown in the table.

• Select Stop to stop taking sample readings.

After the data are collected, the following manipulations can be done on the graphic data:

The Time Course Program is complete, if

• the sound is turned on, the instrument beeps when the readings are done

• the graph is shown fullsize,

• the scaling of the x-axis occurs automatically,

• the Cursor functions in the vertical navigation bar are

highlighted.

6.6.3.1 Navigation of a time scan or a time scan analysis

After a time scan has been completed, the time and the absorbance/transmittance data are displayed as a curve.

Where the cursor is positioned on the curve, the elapsed time up to this point and the corresponding absorbance are highlighted.

Table 15 Navigating the time scan

Advanced Operations

Cursor Function/ Zoom Function

Curve Icon (Choice of Cursor Mode)

Arrow keys

Zoom Icon

Description

Delta mode: A second cursor is highlighted. The position of the fixed cursor was previously

defined in Cursor Mode Single. Use the active cursor to select any point on the measurement curve. The difference to the fixed cursor is shown on the curve. The delta values are correspondingly highlighted and displayed on the x and y axes. The gradient of the curve and the correlation coefficient (r Delta mode are shown under the curve.

Cursor Mode Single: The cursor moves to each selected measurement point of the scan. The arrow keys (right/left) are used to move the cursor (depending on the selected mode) to the

next data point. The data of the data point (wavelength/absorbance or transmittance value) are highlighted on the x and y axes.

Note: Press any point on the curve to display the associated data.

This function is used to magnify the section of the curve in the vicinity of the cursor. The original curve size can be restored by pressing the zoom icon again.

6.7 System checks

) between the cursor points in the

1. Press System Checks in the Main Menu.

The System Checks menu contains instrument information and various performance tests.

Advanced Operations

6.7.1 Instrument information

1. Press Instrument Information in the System Checks menu.

2. The model, serial number and software version are displayed.

6.7.2 Upgrade of the instrument software

To obtain the software for the update from the Internet at

www.hach.com:

1. Go to http://www.hach.com.

2. On the DR 2800 product page, click Lab System Software/Software Update Downloads under Downloads.

3. Locate the appropriate download and follow the prompts for

saving the file(s) to the USB memory stick.

4. On the DR 2800, press Instrument Update in the System Checks menu.

5. Connect the USB stick to the USB interface on the DR 2800 (section 3.4 on page 13). Press OK.The link is established automatically and the software is updated.

6. Press OK to return to the System Checks menu.

Note: When the instrument software has been updated, a prompt to restart the instrument is displayed.

6.7.3 Optical checks

6.7.3.1 Wavelength check

Advanced Operations

For each optical check, the measured results must be evaluated against user-specific requirements. The check options do not define tolerances.

1. Press Optical Checks in the System Checks menu.

The Optical Checks menu contains programs for checking the wavelength accuracy, stray light and photometric accuracy.

An optional test filter set (Verification Kit) (Section 9 on page 119) containing 6 precision glass filters, target values, tolerances and instructions is available and is recommended as an aid for carrying out comprehensive in-house instrument checks.

The Wavelength Check test is used to check wavelength accuracy at 807 nm.

1. Press Wavelength Check in the Optical Checks menu.

2. Insert the adapter (A) for 10-mm rectangular cells in the cell

compartment #2 and insert the sample cuvette/cell (Neodym or BG20/2) in the adapter. Close the cell compartment. Press Start.

3. The result is displayed and is to be compared with the nominal/standard data (given in the quality control certificate) of the sample cuvette/cell.

4. Press Cancel to return to Optical Checks.

Advanced Operations

6.7.3.2 Stray light check

The Stray Light test is used to measure the stray light in the instrument at 340 nm.

1. Press Stray Light Check in the Optical Checks menu.

2. Remove any cuvette/sample cell from the cell compartment.

3. Insert the adapter (A) for 10-mm rectangular cells in the cell compartment #2. Press Zero.

4. Insert the sample cuvette/cell or reference filter into the cell compartment #2. Close the cell compartment. Press Start.

100

5. The ”Average“ and ”Std Deviation“ are calculated from

100 successive absorbance measurements. The result is displayed and is to be compared with the nominal/standard data (given in the quality control certificate) of the sample cuvette/cell.

Note: Failings and passings will be defined by the user.

6. Press Cancel to return to Optical Checks.

Hach DR 2800 User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual

Table of Contents

Section 1 Specifications

Section 2 General Information

2.1 Safety information

2.1.1 Use of hazard information

2.1.2 Precautionary labels

2.1.3 Class 1 LASER

2.1.4 Chemical and Biological Safety

2.2 Overview of product

Section 3 Installation

3.1 Unpack the instrument

3.2 Operating environment

3.3 Power connections

3.4 Connection

3.5 Cell compartments, Cell adapters, Light Shield and Protective Cover

3.5.1 Cell compartments and adapters

3.5.2 Installation of the cuvette/sample cell adapters

3.5.3 Use of the light shield for measurements

3.5.4 Protective Cover

3.6 Mobile use of the DR 2800 for field analysis

3.6.1 Position the Protective Cover

3.7 Beam path

Section 4 Start Up

4.1 Power the instrument on and off

4.2 Language selection

4.3 Self-Check

Section 5 Standard Operations

5.1 Overview

5.1.1 Tips for the use of the touch screen

5.1.2 Use of the alphanumeric keypad

5.1.3 Main Menu

5.2 Instrument Setup mode

5.2.1 Operator ID

5.2.2 Sample ID

5.2.3 Date and time

5.2.4 Display and sound preferences

5.2.5 Power Management

5.2.6 PC and printer

5.2.6.1 Printer setup

5.2.6.2 Print data

5.2.6.3 HACH Data Trans

5.2.7 Password

5.2.7.1 Password deactivation

5.3 Store, recall, send and delete data

5.3.1 The data log

5.3.1.1 Auto/manual data storage

5.3.1.2 Recall stored data from the data log

5.3.1.3 Send data from the data log

5.3.1.4 Delete stored data from the data log

5.3.2 Time Course

5.3.2.1 Data Storage from Time Course

5.3.2.2 Recall Stored Data from Time Course

5.3.2.3 Send Data from Time Course

5.3.2.4 Delete Stored Data from Time Course

5.4 Stored Programs

5.4.1 Select a saved test/method; enter user-specific basic data

5.4.2 Stored program options

5.4.3 Use of program timers

5.4.4 Set the dilution factor

5.4.5 Run a standard adjust

5.4.6 Set the chemical form

5.4.6.1 Change of the default setting of the chemical form

5.4.7 Run a reagent blank

5.4.8 Analysis of samples

5.4.9 Add stored programs to the favorite programs list

5.5 Barcode Programs

5.5.1 Complete a barcode 13 mm test/vial

5.5.2 Select the measuring range

5.5.3 Select the chemical evaluation form

5.5.3.1 Change of the default setting of the chemical form

5.5.4 Basic test-specific and sample-specific data settings

5.5.5 Sample blank

5.5.6 Update/edit barcode tests

5.5.6.1 Manual update of a barcode test

5.5.6.2 Update an existing barcode test

5.5.6.3 Program a new test