Hach BioTector B3500C User Manual

Catalog Number: 10-MAT-452

BioTector B3500C Online TOC Analyzer

USER MANUAL

November 2012, Edition 1

BS5AX 2.02.01a

© Copyright BioTector 2012. All rights reserved. Printed by BioTector. Printed in the Republic of Ireland.

Table of Contents

SECTION 1 SAFETY PRECAUTIONS .......................................................................... 5

1.1 INFORMATION AND SAFETY SIGNS USED IN THE MANUAL ....................................................... 5

1.2 PRECAUTIONARY LABELS ATTACHED TO THE INSTRUMENT .................................................... 6

1.3 CERTIFICATION MARKS ATTACHED TO THE INSTRUMENT ....................................................... 7

1.4 POTENTIAL SYSTEM SAFETY HAZARDS ................................................................................ 8

1.4.1 Ozone and Toxicity .........................................................................................................................9

1.4.2 First Aid Treatment .........................................................................................................................9

1.5 GENERAL SAFETY PRECAUTIONS ...................................................................................... 10

1.5 PRÉCAUTIONS GÉNÉRALES DE SÉCURITÉ ........................................................................... 10

1.5.1 Electrical and Burn Precautions .................................................................................................. 11

1.5.1 Précautions relatives à l’électricité et aux brûlures ..................................................................... 11

1.5.2 Carrier Gas and Exhaust Gas Precautions ................................................................................. 12

1.5.2 Précautions relatives au gaz porteur et d'échappement ............................................................. 12

1.5.3 Chemical Precautions ................................................................................................................. 13

1.5.3 Précautions chimiques ................................................................................................................ 13

1.5.4 Sample Stream Precautions ........................................................................................................ 14

1.5.4 Précautions relatives aux échantillons ........................................................................................ 15

SECTION 2 OPERATOR’S MANUAL ......................................................................... 16

2.1 SOFTWARE SCREENS AND SOFTWARE MENU DIAGRAM ............................................................ 16

2.1.1 Startup State ................................................................................................................................ 18

2.1.2 System Status Messages ............................................................................................................ 18

2.1.3 Analysis Data Screen .................................................................................................................. 19

2.1.4 Analysis Graph Screen ................................................................................................................ 20

2.1.5 Reagent Status Screen ............................................................................................................... 20

2.1.6 Select Level Menu ....................................................................................................................... 21

2.1.7 Enter Password Menu ................................................................................................................. 21

2.2 OPERATION MENU ............................................................................................................ 22

2.2.1 Start Stop ..................................................................................................................................... 22

2.2.2 Reagents Setup ........................................................................................................................... 24

2.2.2.1 Install New Reagents ......................................................................................................................... 24

2.2.2.2 Purge Reagents & Zero ..................................................................................................................... 25

2.2.3 System Range Data Screen ........................................................................................................ 25

2.2.4 Manual Program Menu ................................................................................................................ 26

2.2.5 Reaction Archive Screen ............................................................................................................. 27

2.2.6 Fault Archive Menu ..................................................................................................................... 27

2.2.7 Time & Date Menu ...................................................................................................................... 28

2.2.8 Contact Information ..................................................................................................................... 28

2.2.9 LCD Adjust .................................................................................................................................. 28

2.3 CALIBRATION MENU.......................................................................................................... 29

2.3.1 Zero Calibration ........................................................................................................................... 29

2.3.2 Span Calibration .......................................................................................................................... 31

SECTION 3 TECHNICAL SPECIFICATIONS.............................................................. 34

SECTION 4 INTRODUCTION ...................................................................................... 37

4.1 BIOTECTOR MAJOR COMPONENTS .................................................................................... 37

4.1.1 Analysis Enclosure ...................................................................................................................... 37

4.1.2 Motherboard Enclosure ............................................................................................................... 40

4.2 BIOTECTOR OPERATION ................................................................................................... 42

4.2.1 BioTector Oxidation Method ........................................................................................................ 42

4.2.2 BioTector Sample Injection ......................................................................................................... 43

4.2.3 BioTector Oxygen Concentrator .................................................................................................. 45

4.2.4 BioTector Analysis Types ............................................................................................................ 47

4.2.4.1 TIC & TOC Analysis ........................................................................................................................... 47

4.2.4.2 TC Analysis ........................................................................................................................................ 47

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4.2.4.3 VOC (POC) Analysis ......................................................................................................................... 48

SECTION 5 INSTALLATION ....................................................................................... 49

5.1 BASIC SYSTEM REQUIREMENTS ........................................................................................ 49

5.2 UNPACKING AND INSTALLATION ......................................................................................... 50

5.2.1 Analyzer Dimensions and Mounting ............................................................................................ 51

5.2.2 Wiring Power and Signal Terminals ............................................................................................ 53

5.2.3 Wiring External Power Disconnection Switches .......................................................................... 55

5.2.4 System Fuse Specifications ........................................................................................................ 57

5.3 AIR SUPPLY AND REAGENT CONNECTIONS......................................................................... 58

5.3.1 Air Supply Connection ................................................................................................................. 59

5.3.2 Reagent Connections .................................................................................................................. 60

5.4 SAMPLE, DRAIN AND EXHAUST CONNECTIONS ................................................................... 62

5.4.1 Sample Inlet Tube Position ......................................................................................................... 62

5.4.2 Drain and Exhaust Connections .................................................................................................. 64

SECTION 6 REAGENTS AND CALIBRATION STANDARDS.................................... 65

6.1 REAGENTS ....................................................................................................................... 65

6.1.1 Sulfuric Acid Reagent .................................................................................................................. 66

6.1.2 Sodium Hydroxide Reagent ........................................................................................................ 68

6.2 CALIBRATION STANDARDS ................................................................................................ 70

SECTION 7 ANALYZER COMMISSIONING AND STARTUP .................................... 73

SECTION 8 MAINTENANCE MENU ........................................................................... 78

8.1 DIAGNOSTICS MENU .................................................................................................... 79

8.1.1 Process Test ................................................................................................................................ 80

8.1.1.1 Pressure Test .................................................................................................................................... 80

8.1.1.2 Flow Test ........................................................................................................................................... 81

8.1.1.3 Ozone Test ........................................................................................................................................ 82

8.1.1.4 Sample Pump Test ............................................................................................................................ 84

8.1.1.5 pH Test .............................................................................................................................................. 85

8.1.2 Simulate ....................................................................................................................................... 89

8.1.3 Signal Simulate............................................................................................................................ 93

8.1.4 Data Output ................................................................................................................................. 95

8.1.4.1 Send Reaction Archive ...................................................................................................................... 96

8.1.4.2 Send Fault Archive ............................................................................................................................ 99

8.1.4.3 Send Configuration ............................................................................................................................ 99

8.1.4.4 Send All Data ..................................................................................................................................... 99

8.1.5 Input/Output Status ................................................................................................................... 100

8.1.6 Oxygen Controller Status .......................................................................................................... 101

8.1.7 Service ....................................................................................................................................... 102

8.2 COMMISSIONING MENU ............................................................................................ 103

8.2.1 Reaction Time ........................................................................................................................... 103

8.2.2 Sample Pump ............................................................................................................................ 104

8.2.3 Stream Program ........................................................................................................................ 104

8.2.4 COD/BOD Program ................................................................................................................... 105

8.2.5 New Reagents Program ............................................................................................................ 106

8.2.6 Reagents Monitor ...................................................................................................................... 106

8.2.7 Autocal Program ........................................................................................................................ 107

8.2.8 4-20mA Program ....................................................................................................................... 107

8.2.9 Alarm Program .......................................................................................................................... 109

8.2.10 Data Program ........................................................................................................................ 110

8.2.11 Information ............................................................................................................................ 110

8.3 SYSTEM CONFIGURATION MENU ............................................................................. 111

8.3.1 Analysis Mode ........................................................................................................................... 112

8.3.2 System Program ........................................................................................................................ 113

8.3.3 Calibration Data ......................................................................................................................... 118

8.3.4 Sequence Program ................................................................................................................... 118

8.3.4.1 Average Program ............................................................................................................................. 118

8.3.4.2 Zero Program ................................................................................................................................... 119

8.3.4.3 Span Program .................................................................................................................................. 120

8.3.4.4 Reagents Purge ............................................................................................................................... 122

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8.3.4.5 Pressure/Flow Test Program ........................................................................................................... 122

8.3.5 Output Devices .......................................................................................................................... 123

8.3.5.1 System Outputs ............................................................................................................................... 123

8.3.5.2 Programmable Outputs .................................................................................................................... 125

8.3.6 Reaction Check ......................................................................................................................... 126

8.3.7 Result Integration ...................................................................................................................... 127

8.3.8 Fault Setup ................................................................................................................................ 128

8.3.9 Fault Status ............................................................................................................................... 131

8.3.10 CO2 Analyzer ........................................................................................................................ 132

8.3.11 Cooler Program ..................................................................................................................... 133

8.3.12 Ozone Destructor Program ................................................................................................... 134

8.3.13 Software Update ................................................................................................................... 135

8.3.14 Password .............................................................................................................................. 136

8.3.15 Language .............................................................................................................................. 136

8.3.16 Hardware Configuration ........................................................................................................ 136

SECTION 9 TROUBLESHOOTING OF SYSTEM FAULT, WARNING AND

NOTIFICATION EVENTS ................................................................................................ 137

9.1 BIOTECTOR FAULT EVENT EXPLANATION AND REMEDIAL ACTION ...................................... 137

9.2 BIOTECTOR WARNING EVENT EXPLANATION AND REMEDIAL ACTION ................................. 141

9.3 BIOTECTOR NOTIFICATION EVENT EXPLANATION AND REMEDIAL ACTION .......................... 149

SECTION 10 SERVICE AND MAINTENANCE ............................................................ 150

10.1 WEEKLY MAINTENANCE .................................................................................................. 150

10.2 SIX MONTH SERVICE ...................................................................................................... 151

SECTION 11 SYSTEM REPLACEMENT AND SPARE PARTS .................................. 155

SECTION 12 GENERAL INFORMATION .................................................................... 158

12.1 DECLARATION OF CONFORMITY AND CERTIFICATION ........................................................ 158

12.2 DECLARATION OF COMPLIANCE ....................................................................................... 159

12.3 WARRANTY AND EXCLUSIONS ......................................................................................... 160

SECTION 13 APPENDICES ......................................................................................... 161

APPENDIX 1 GLOSSARY OF TERMS AND ABBREVIATIONS .......................................................... 161

APPENDIX 2 CONTACT INFORMATION ...................................................................................... 164

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Used to indicate supplementary information, to call attention to
recommendations, to simplify the operation and to guarantee the correct use
of the equipment.

Used when there is a danger of minor damage to the system if the user does
not follow precautions.

Used when there is a danger of minor injury or serious damage to the
system if the user does do not follow the precautions.

Used when failure to observe a safety precaution may result in serious injury
or death.

Caution

WARNING

DANGER

Section 1 Safety Precautions

Please read this manual before unpacking, setting up, or operating the BioTector.
BioTector should only be used by qualified trained staff and for the purpose it is intended for. Do not use or

install this equipment in any way other than the methods specified in this manual. The procedures and
methods described in this manual are based on assuming the user have basic, fundamental background on
electronics, chemistry and analyzer equipment.

If the instructions in this manual are not followed, the operation and protection provided by the
equipment may be impaired.

1.1 Information and Safety Signs used in the Manual

When any supplementary information is required and if any hazards exist, the necessary information and
safety signs (Information, Caution, Warning and Danger) will be displayed for the corresponding section or
procedure in this manual.

Page 5

This symbol, when displayed on the instrument, indicates that the user
must gather the necessary operation and/or safety information given in the
instruction manual.

This symbol, when attached on an enclosure, indicates an existing risk of
electrical shock and/or electrocution. Only qualified personnel should open
such enclosures and work with hazardous voltages.

This symbol, when displayed on a component, identifies that the
component surface can be hot. When it is necessary to work with this
component, it should be handled with care.

This symbol, when noted on a product, illustrates the risk of chemical harm
due to its corrosive, acidic, caustic or solvent nature. Only qualified and
trained staff should handle such chemicals.

This symbol, when displayed on the instrument, indicates the presence of
devices sensitive to Electro-Static Discharge (ESD). Prior to any work with
such components, the individual should be grounded via an earth strap to
prevent any possible damage.

This symbol, when displayed on the product, indicates that protective eye
wear must be used during the maintenance or service of the equipment.

This symbol, when used on the product, identifies the location of the
protective earth (ground) connection.

This symbol, when displayed on the instrument, indicates that the user
must follow the necessary local, state and federal laws during the disposal
of the display.

1.2 Precautionary Labels Attached to the Instrument

The labels and tags attached to the instrument are summarized below. Please read all labels and tags
attached to the instrument. If not observed, personal injury or damage to the instrument could occur.

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This mark, which stands for European Conformity "Conformité
Européene", indicates that “The instrument complies with the

European product directives, health, safety and
environmental protection legislations”. See Section 12.1

Declaration of Conformity and Certification for details.

If these marks are displayed on the instrument, they indicate that

“This product has been tested to the Safety Requirements for
Electrical Equipment for Measurement, Control and
Laboratory Use; Part 1: General Requirements of ANSI/UL
61010-1 and CAN/CSA-C22.2 No. 61010-1, second edition, 12
July 2004 including revision 22 July 2005”. Intertek ETL listed

mark, which stands for Electrical Testing Laboratories, identifies
that the product has been tested by Intertek, found in compliance
with accepted national standards, and it meets the minimal
requirements required for sale or distribution.

1.3 Certification Marks Attached to the Instrument

The certification marks attached to the instrument and their meanings are summarized below.

Page 7

Maintenance and operation should not be carried out unless personnel have been fully
trained in the operation of the BioTector.
Prior to working on the inside of the analyzer, the technician should be grounded via an
earth strap.

Caution

1.4 Potential System Safety Hazards

The potential safety hazards, which are associated with a running BioTector system, are as follows:

 Electrical hazards
 Potentially hazardous chemicals
 Oxygen gas and components generating Ozone gas

Please read the instructions in this manual carefully before installing or starting the BioTector.
The manufacturer cannot accept liability for damages due to non-observance of this manual. Use of spare

parts not supplied by the manufacturer will invalidate the warranty. If the equipment is used in a manner not
specified by the manufacturer, the protection provided by the equipment may be impaired. The manufacturer
shall not be liable for omissions or errors contained herein or for incidental or consequential damages in
connection with the furnishing, performance, or use of this material.

The information contained in this manual is subject to change without notice.
The information contained herein is protected by copyright. Reproduction, adaptation, or translation of any

part of this manual without prior written permission is prohibited, except as allowed under the copyright laws.
Product names mentioned herein are for identification purposes only and may be trademarks or registered

trademarks of their respective companies.
Where manuals are translated into several languages, the source language text is considered as the original.

Page 8

Terms

Properties of Ozone (O3)

Molecule Weight

47.9982 g/g-mol

Boiling Point

-119  0.3 °C

Melting Point

-192.7  0.2 °C

1.4.1 Ozone and Toxicity

Ozone is found in gaseous form as a natural ingredient of the earth's atmosphere. It is not a poisonous
chemical but a strong oxidizing chemical. Some of the chemical and physical properties of ozone are as
follows:

Exposure to even low concentrations of ozone can be damaging to delicate nasal, bronchial and pulmonary
membrane. Symptoms of acute ozone toxification appear at a concentration of about 1 ppm by volume. The
type and severity of symptoms depend on the concentration and duration of exposure. In mild cases and in
the early phases of severe cases, symptoms will include one or more of the following:

 Irritation or burning of the eyes, nose or throat
 Lassitude
 Frontal headache
 Sensation of sub-sternal pressure
 Constriction or oppression
 Acid taste in mouth
 Anorexia

In more severe cases, the symptoms may include dyspnoea, cough, choking sensation, tachycardia, vertigo,
lowering of blood pressure, severe cramping, chest pain, and generalized body pain. Pulmonary oedema may
develop with delayed onset, usually one or more hours after exposure.

Following severe acute ozone toxification, recovery is slow. In the few severe human cases reported, 10 -14
days of hospitalization were required. In these cases, minimal residual symptoms were present for as long as
9 months, but all cases eventually recovered completely.

The 1983 ACGIH has recommended a Threshold Limit Value (TLV) of 0.1 ppm (0.2 mg/m3) for ozone. The
safe level for short human exposure to concentrations of ozone in excess of 0.1 ppm (Threshold Limit Value)
is not known with certainty. The atmospheric concentration immediately hazardous to life is likewise not
known, but inhalation of 50 ppm for 30 minutes would probably be fatal. The odor threshold of ozone for a
normal person is 0.01 - 0.02 ppm by volume in air.

1.4.2 First Aid Treatment

Move the victim to an uncontaminated atmosphere. Control restlessness and pain by the administration of
sedatives and anodynes orally. Severe cases may require subcutaneous injections of small doses of
meperidine hydrochloride (Demerol) for relief of pain. Give oxygen inhalation by facemask when the acute
symptoms have subsided. Severe cases require hospitalization since deferred pulmonary oedema may
develop.

Page 9

1.5 General Safety Precautions

Please pay attention to all caution, warning and danger statements at all times. Non-observance of the safety
instructions can result in serious personal injury, death or damage to the equipment. Therefore observe the
following:

 Only engineers trained by the manufacturer should carry out maintenance on the BioTector.
 The power supplies contain capacitors that are charged to hazardous voltages. After disconnecting

the main power, allow a minimum of one minute for discharge before opening the control section.

 Never wash or spray the system with water. Do not allow water to enter the interior.
 Protect the system from one-sided heat radiation, direct sunlight and vibration. System must be

installed in a dry, dust-free room. Special precautions are required in environments with corrosive
gases, vapors or explosion risk.

 Please do not place anything on top of the system.

1.5 Précautions générales de sécurité

Prière d’être toujours attentif à toutes les notices de prudence, d’avertissement ou de danger. Le non respect
des instructions de sécurité peut engendrer la blessure grave d’individus, leur décès ou la dégradation du
matériel. Pour ces raisons, prière d’observer les règles suivantes:

 Seuls les ingénieurs formés par le fabricant doivent réaliser des travaux de maintenance sur le

BioTector.

 L’alimentation électrique contient des condensateurs qui sont chargés à des tensions dangereuses.

Après avoir débranché l’alimentation électrique, attendre au moins une minute pour permettre la

décharge avant d'ouvrir le boîtier de commande.

 Ne jamais laver ou arroser l’appareil avec de l’eau. Ne pas laisser de l’eau pénétrer à l’intérieur.
 Protéger l’appareil des radiations de chaleur sur un seul côté, des rayons directs du soleil et des

vibrations. L’appareil doit être installé dans une pièce sèche et sans poussière. Il est nécessaire de
prendre des précautions particulières dans les environnements contenant des vapeurs ou gaz
corrosifs ou ceux à risque d’explosion.

 Prière de ne rien poser sur le dessus de l'appareil.

Page 10

BioTector contains electrical components operating under high voltages.
Contact may result in electric shock and severe or fatal injury.

BioTector contient des composants électriques qui fonctionnent à des tensions
élevées. Un contact peut engendrer un choc électrique et des blessures graves
ou mortelles.

DANGER

DANGER

1.5.1 Electrical and Burn Precautions

During system installation, maintenance or servicing:

 Isolate the system power lines before starting any work in the electronic enclosure.
 All electrical work should be carried out by qualified electrical personnel only.
 Comply with all local and national regulations when working with electrical connections.
 Make sure the system is properly earthed (grounded) before switching on.
 It is recommended to connect the mains through an external isolator (2-pole disconnection switch),

and if possible connect the mains through an earth leakage circuit breaker.

 When working with hot surfaces, use protective gloves and handle the components with care.

1.5.1 Précautions relatives à l’électricité et aux brûlures

À l’installation de l’appareil, sa maintenance ou son entretien:

 Isoler les fils électriques de l’appareil avant de commencer tout travail dans le boîtier électronique.
 Seul le personnel électricien qualifié est habilité à effectuer tous travaux d’électricité.
 Se conformer aux règlementations locales et nationales pour tout travail sur un branchement

électrique.

 Avant de l’allumer, veiller à la bonne mise à la terre de l’appareil.
 Il est recommandé de brancher l’alimentation électrique de réseau par le biais d’un isolateur externe

(commutateur bipolaire de découplage) et si possible, brancher l'alimentation de réseau en la faisant
passer par un disjoncteur de fuite à la terre.

 Utiliser des gants de protection pour les travaux sur les surfaces très chaudes et prendre soin en

manipulant les composants.

Page 11

1.5.2 Carrier Gas and Exhaust Gas Precautions

BioTector uses oxygen (O2) gas as the carrier gas during its operation. The oxygen gas must be free of
carbon dioxide (CO2) and nitrogen (N2) gases. The average rate of oxygen consumption in BioTector is 29 l/h
(483 ml/min). Carbon dioxide filtered air, carbon dioxide and nitrogen contaminated oxygen gas are not
suitable for BioTector TOC analyzer. When handling oxygen:

 The same precautions, which are required for any high pressure or compressed gas system, must be

taken to avoid accidents.

 Comply with all local and national regulations and/or manufacturer's recommendations and guidelines

when working with oxygen.

 If oxygen cylinders are used, they must be transferred safely using appropriate equipment (e.g. carts,

hand trucks etc.)

 If oxygen cylinders are used, they should be labeled clearly for identification and well secured for

storage and transport.

 Avoid the use of extensive number of adaptors and couplers.
 Do not allow oxygen to come in direct contact with grease, oil, fat, and other combustible materials. If

uncertain how to handle oxygen cylinders and high concentration oxygen, contact your local oxygen
manufacturer.

 If oxygen concentrator is used, take precautions to avoid a fire in the area of the concentrator, install

the concentrator only in a well ventilated area and comply with all local and national regulations.

Vent waste gases to atmosphere or to a well ventilated area making the necessary connections on system
exhaust. Under normal operating conditions, waste gases will contain oxygen, traces of carbon dioxide and
the traces of volatiles/gases which may exist in the sample stream. Under abnormal conditions, the waste
gases may contain traces of ozone.

1.5.2 Précautions relatives au gaz porteur et d'échappement

Pour son fonctionnement, BioTector emploie de l’oxygène (O

comporter aucun gaz carbonique (CO2) ni d’azote (N2). Le taux moyen de consommation d’oxygène du
BioTector est de 29L/heure (483 ml/min). L’analyseur BioTector TOC ne tolère pas l’air filtré de gaz
carbonique ni l'oxygène contaminé de gaz carbonique et d'azote. À la manipulation de l’oxygène:

 Afin d’éviter les accidents, prendre les mêmes précautions que pour tout appareil à haute pression ou

gaz comprimé.

 Pour toute opération avec de l’oxygène, se conformer aux règlementations locales et nationales et/ou

aux recommandations et consignes du fabricant.

 S’ils sont employés, les cylindres d’oxygène doivent être transportés en toute sécurité à l’aide du

matériel approprié (chariots, diables, etc.)

 S’ils sont employés, les cylindres d’oxygène doivent être clairement étiquetés pour en permettre

l’identification et bien arrimés pour leur stockage et leur transport.

 Éviter d’utiliser un nombre élevé d’adaptateurs et de dispositifs de couplage.
 Ne pas laisser l’oxygène entrer en contact direct avec de la graisse, de l’huile, des matières grasses

ou d’autres matières combustibles. Veuillez contacter votre fabricant local d’oxygène si vous avez
des doutes sur la manière de manipuler les cylindres d’oxygène et l’oxygène de haute concentration.

 Dans le cas où un concentrateur est employé, prendre les précautions nécessaires pour éviter un

incendie dans la zone du concentrateur, n’installer le concentrateur que dans un endroit bien ventilé

et se conformer aux règlementations locales et nationales.

Évacuer les gaz usés dans l’atmosphère ou dans un endroit bien ventilé en réalisant les branchements
voulus sur l'échappement de l’appareil. Dans des conditions normales de fonctionnement, les gaz usés
contiennent de l’oxygène, des traces de gaz carbonique et des traces de composants volatiles/gaz qui
peuvent être présents dans l’échantillon. Dans des conditions anormales, les gaz usés peuvent contenir des
traces d’ozone.

) comme gaz porteur. L’oxygène ne doit

2

Page 12

1.5.3 Chemical Precautions

A number of chemicals and compounds to be used with BioTector are listed in Section 6 Reagents

and Calibration Standards. Some of these compounds are harmful, corrosive, acidic and oxidizing.

Appropriate precautions must be taken when handling these chemicals or solutions prepared from these
chemicals.

Physical contact with these chemicals and inhalation of any vapors must be minimized using appropriate
safety equipment.

1.5.3 Précautions chimiques

La liste de la Section 6 Réactifs et Standards de Calibration (Section 6 Reagents and Calibration

Standards) énumère un certain nombre de produits chimiques et composés à utiliser avec BioTector.

Certains de ces composés sont nocifs, corrosifs, acides et oxydants. Il est essentiel de prendre les
précautions appropriées lors de la manipulation de ces produits chimiques ou des solutions dont ils sont la
base.

Il est essentiel d’employer l’équipement de sécurité approprié afin de minimiser le contact direct avec ces
produits chimiques et l’inhalation de toutes vapeurs.

Page 13

Component

Material

Tubing

PFA (Per-fluoro-alkoxy)
Vinyl
Reinforced Silicon
Reinforced PVC (Poly-vinyl-chloride)

Fittings

PFA (Per-fluoro-alkoxy)
Stainless Steel (SS-316)
PVDF (Poly-vinylidene-flouride)
Brass

Pump Tubing

EMPP (Elastomer-modified-poly-propylene)

Connectors

PP (Poly-propylene)
HDPE (High-density-poly-ethylene)

Connector & Valve Tubing

EMPP (Elastomer-modified poly-propylene)
Viton / FKM (Fluoro-elastomer)

Sample Valve

PEEK (Poly-ether-ether-ketone)
PVDF (Poly-vinylidene-flouride)
Stainless Steel (SS-316)
EMPP (Elastomer-modified poly-propylene)

Reactor

Hastelloy (C-276)
Stainless Steel (SS-316)
PFA (Per-fluoro-alkoxy)
PTFE (Poly-tetra-fluoro-ethylene)

Valve Seals

Kalrez / FFKM (Perfluoro-elastomer)
Viton / FKM (Fluoro-elastomer)
NBR (Nitrile-butadiene-rubber)

Oxidized Sample Catch-pot/Cleaning Vessel

Borosilicate Glass

NDIR CO2 Analyzer

Hastelloy (C-276)
Stainless Steel (SS-316)

NDIR CO2 Analyzer Lens

Sapphire

1.5.4 Sample Stream Precautions

The user is responsible to establish the potential hazard associated with each sample stream. Necessary
precautions must be taken, to avoid physical contact with any harmful sample stream, which may contain
chemical or biological hazards.

System components and their composition, which come in contact with the sample liquid and possible volatile
gases from the sample, are tabulated in table 1 below. If there are suspected compatibility issues between the
sample stream and BioTector components, please contact the manufacturer or the distributor.

Table 1 System components and their composition

Page 14

Composant

Matière

Tuyauterie

PFA (perfluoroalkoxy)
Vinyle
Silicone renforcé
PVC renforcé (poly-chlorure de vinyle)

Raccords

PFA (perfluoroalkoxy)
Acier inoxydable (SS-316)
PVDF (polyfluorure de polyvinylidène)
Laiton

Tuyauterie de la pompe

PPMOD (polypropylène modifié par élastomère)

Connecteurs

PP (polypropylène)
HDPE (polyéthylène haute densité)

Tuyauterie des connecteurs & vannes

PPMOD (polypropylène modifié par élastomère)
Viton / FKM (élastomère fluoré)

Vanne d’entrée de l’échantillon

PEEK (polyéther éther cétone)
PVDF (polyfluorure de polyvinylidène)
Acier inoxydable (SS-316)
PPMOD (polypropylène modifié par élastomère)

Réacteur

Hastelloy (C-276)
Acier inoxydable (SS-316)
PFA (perfluoroalkoxy)
PTFE (polytetrafluoroethylene)

Joints des vannes

Kalrez / FFKM (élastomère perfluoré)
Viton / FKM (élastomère fluoré)
NBR (caoutchouc nitrile butadiène)

Bac de récupération/récipient de nettoyage de
l’échantillon oxydé

Verre borosilicaté

Analyseur infrarouge de CO2

Hastelloy (C-276)
Acier inoxydable (SS-316)

Lentille de l’analyseur infrarouge de CO2

Saphir

1.5.4 Précautions relatives aux échantillons

L’usager assume la responsabilité d’établir le danger possible que représente chaque échantillon. Il est
essentiel de prendre les précautions voulues afin d’éviter le contact physique avec tout échantillon nocif qui

pourrait présenter un danger chimique ou biologique.
Le tableau 1 ci-dessous présente les composants de l’analyseur (et leur composition) qui entrent en contact

avec l’échantillon liquide et les éventuels gaz volatiles émanant de l’échantillon. Si vous soupçonnez des
problèmes de compatibilité entre l’échantillon et les composants BioTector, veuillez contacter le distributeur

ou le fabricant.
Tableau 1 Composants de l’analyseur et leur composition

Page 15

<

Selector. Used to designate the menu item being selected.

*

Highlighter. Used to highlight an active or ongoing function of the BioTector.

_

Blinking Cursor. Used to indicate current user position when setting changes are
being made.

Section 2 Operator’s Manual

2.1 Software Screens and Software Menu Diagram

The BioTector is equipped with a built-in microprocessor, which has been programmed to enable the user to
control the instrument using just six buttons of its membrane keypad. By pressing the appropriate button, the
user can move through the various levels of the software menu.

The functions of the 6 keys on the membrane keypad are described below:
The ESCAPE [  ,  ,  ] key, which returns user to the previous screen, can also be used to cancel

programming entries. If the ESCAPE key is pressed for longer than 1 second the user returns to the main
menu.

The LEFT [  ,  ] and RIGHT [  ,  ] arrow keys are used for numerical entries and programming the
BioTector.

The UP [  ,  ] and DOWN [  ,  ] arrow keys are used for numerical entries and programming the
BioTector.

The ENTER [  , √ ] key, which advances user to the next screen, is also used to enter programmed
settings in the BioTector.

The symbols used on BioTector LCD screen and their meanings are as follows:

There are three main menu levels in BioTector in addition to the analysis graph, analysis data and reagent
status screens:

 Level 1 – Operation: This level controls the basic operation of the BioTector and allows access to

the archives.

 Level 2 – Calibration: This level allows the user to run zero and span calibration cycles.
 Level 3 – Maintenance: This level allows the user to test the individual components of the BioTector

for diagnostics, to download data, to program the software functions and to program the system
specific settings in the BioTector.

Page 16

Software Menu Diagram

Page 17

2.1.1 Startup State

When the BioTector is powered up, its LCD screen will automatically display the Analysis Data screen after a
delay of 60 seconds.

By pressing the ESCAPE key the user moves from the Analysis Data screen to the Analysis Graph screen.
Pressing the ENTER key on the Analysis Graph screens brings the user back to the Analysis Data screen.

Pressing ENTER key on the Analysis Data screen will bring up the Select Level screen, from where the user
can select the desired menu level using the UP or DOWN and ENTER keys.

Entry to each menu level can be controlled by numerical passwords. If the passwords are not set, pressing
the enter key will bring the user directly to the sub menu screen of the selected level. If the system has been
set up with passwords, the Password menu will appear and the password must be entered before access to
the selected level is allowed.

In all cases, pressing the ESCAPE key will return the user to the previous screen.

2.1.2 System Status Messages

The system status messages are displayed on the top left hand side of the Analysis Data and Reagent Status
screens. On most other screens, only the screen name is displayed in this location.

System status messages are displayed in the following priority:

1. SYSTEM MAINTENANCE – the BioTector is in Maintenance mode, activated by the maintenance
switch.

2. SYSTEM FAULT – There is a fault on the BioTector.

3. SYSTEM CALIBRATION – The BioTector is calibrating. This could be Span Calibration, Span Check,
Zero Calibration or Zero Check.

4. Running status. This could be one of either:

 SYSTEM RUNNING – system is running.
 SYSTEM STOPPED – system has been stopped by a fault or from the keypad.
 REMOTE STANDBY – system has been put into standby mode remotely.

The BioTector time and date is displayed on the top right side of each screen. When a fault is logged in the
system, a FAULT LOGGED message will alternate with the time/date in this location until the fault has been
corrected.

Changing most system settings are prevented when the BioTector is running.

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B I O T E C T O R R U N N I N G 0 9 : 1 7 : 2 8 1 2 - 0 9 - 0

2

0 9 : 1 3 : 0 2 1 2 - 0 9 - 0 2 R E A C T I O N S T A R T

T I C & T O C S T R E A M 2 R E A C T I O N T Y P E T O C R E A C T I O N P H A S E 1 R A N G E 2 6 6 s R E A C T I O N T I M E

3 6 0 s R E A C T I O N D U R A T I O N R E A C T I O N R E S U L T T I C m g C / l T O C m g C / l 0 9 : 0 7 : 0 2 1 2 - 0 9 - 0 2 S 1 √ 1 3 0 . 0 5 4 0 . 0 0 9 : 0 1 : 0 2 1 2 - 0 9 - 0 2 S 2 √ 3 . 6 3 . 6 0 8 : 5 5 : 0 2 1 2 - 0 9 - 0 2 S 3 √ 7 . 2 7 . 2 0 8 : 4 9 : 0 2 1 2 - 0 9 - 0 2 S 4 x 1 0 . 7 1 0 . 7 0 8 : 4 3 : 0 2 1 2 - 0 9 - 0 2 S 5 x 1 4 . 3 1 4 . 3

0 8 : 3 7 : 0 2 1 2 - 0 9 - 0 2 C F 0 . 9 7 . 9

2.1.3 Analysis Data Screen

The Analysis Data screen is the default display screen on the BioTector for carbon (TIC, TOC, TC and VOC)
analysis. When the user moves through the various levels of the software menu, BioTector returns to this
screen automatically after 15 minutes if there is no further activity on the membrane keypad.

This screen gives information on:

- The Reaction Start time.

- The Reaction Type, for example a TIC & TOC reaction, TC reaction, Cleaning Reaction.

- The Reaction Phase, for example if the reaction is currently in the TIC, Base Oxidation, TOC phase.

- The operation Range (e.g. Range 1, 2 or 3) the BioTector is using to carry out its analysis.

- The Reaction Time, which is the elapsed time (seconds) since the analysis start.

- The Reaction Duration, which is the overall duration (seconds) of the analysis.
The Analysis Data screen also has an archive of the last 25 reactions. The most recent six reactions are

shown on the screen. In order to access the remaining reactions, use the DOWN or RIGHT keys to scroll
down, use the LEFT or UP keys to scroll up.

Each reaction record in the reaction archive contains:

- Start Time - reaction start time.

- Date - reaction date.

- Record Type, using the prefixes below:
S1 to S3 – reactions from stream 1 to stream 3.
M1 to M3 – reactions from manual sample stream 1 to manual stream 3.
√ – sample sensor detected the sample and liquid quality is greater than the threshold value.
x – sample sensor detected no sample or liquid quality is less than the threshold value.
RS – remote standby reaction.
ZC – zero calibration reaction.
ZK – zero check reaction.
ZM – manual zero adjust.
SC – span calibration reaction.
SK – span check reaction.
SM – manual span factor adjust.
A1 to A3 – 24 hours average result from stream 1 to stream 3.

- Analysis Results – analysis results according to the analysis type (e.g. TIC, TOC in mgC/l).

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1 0 1 . 5 [ k P a ] 0 9 : 1 7 : 2 8 1 2 - 0 9 - 0

2

T I C m g u

1 2 . 4 9 5 6 C O 2 T O C m g u

1 5 6 . 4

4 3 5 6 C O 2

0 s 1 2 0 s 2 4 0

s 3 6 0 s

1 0 . 0 l / h 2 6 C 5 6 C O 2 i

1 2 C O 2 z 2 6 5 s

B I O T E C T O R R U N N I N G 0 9 : 1 7 : 2 8 1 2 - 0 9 - 0

2

A C I D M O N I T O R 1 9 . 0 l ~ 2 1 0 D A Y S B A S E M O N I T O R 1 9 . 0 l ~ 2 1 0 D A Y S

N O T E : D A Y S L E F T I S A N E S T I M A T E B A S E D S Y S T E M C U R R E N T U S E

2.1.4 Analysis Graph Screen

The Analysis Graph screen gives information on the current analysis in progress, and allows the user to
monitor the progression of the analysis. This screen gives information on:

- The current atmospheric pressure, measured in kPa (e.g. 101.5 kPa).

- The milligram per liter un-calibrated (mgu) data from the analysis, for example TICmgu or TOCmgu without

any compensation for atmospheric pressure.

- The height of the CO2 peaks in each phase of the reaction (e.g. 956ppm CO2).

- The current MFC flow in l/h (e.g. 10.0 l/h).

- The temperature of the analyzer in °C (e.g. 26°C).

- The CO2 instantaneous value (e.g. 56ppm CO2i) and the CO2 zero value (e.g. 12ppm CO2z) of the

reaction.

- The elapsed time (e.g. 265s) since the start of the analysis.

2.1.5 Reagent Status Screen

If the Reagent Status screen has been activated, the estimated number of days left for each reagent type is
shown on the display.

If the reagents run low, a LOW REAGENTS fault is activated. This fault has to be cleared by resetting the
reagent level in the Install New Reagents menu.

Note that the LOW REAGENTS fault can be set as a warning (where the common fault relay will activate) or a
notification, in which case a special programmable relay is required to signal the LOW REAGENTS condition.

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S E L E C T L E V E L 0 9 : 1 7 : 2 8 1 2 - 0 9 - 0

2

1 < O P E R A T I O N 2 C A L I B R A T I O N 3 M A I N T E N A N C E

0 9 : 1 7 : 2 8 1 2 - 0 9 - 0

2

E N T E R P A S S W O R D F O R

O P E R A T I O N S E C U R I T Y D O M A I N [ 1 2 3 4 ]

2.1.6 Select Level Menu

The Select Level screen allows the user to access the operation, calibration and maintenance menus.

1. Operation. This menu gives access to the basic operation of the BioTector and allows access to the
archives. The level can be password protected using the Password menu.

2. Calibration. This menu allows the user to run zero and span calibration cycles. The level can be
password protected using the Password menu.

3. Maintenance. This menu allows the user to test the individual components of the BioTector for
diagnostics, to download data, to program the software functions and to program the system specific
settings in the BioTector. The sub menus in this level can be password protected using the Password
menu.

2.1.7 Enter Password Menu

The BioTector has separate passwords for all levels/security domains, which are operation, calibration
diagnostics, commissioning, system configuration and hardware configuration.

These passwords are programmable, and if a password has been set up for a particular level in Password
menu (see Section 8.3.14 Password for details), then it must be entered before the BioTector will grant
access to the password-protected security domains.

Use of a higher menu level password also allows access to lower levels/domains.

Page 21

Maintenance should only be carried out when “SYSTEM STOPPED” message

is displayed on the top left corner of the main Analysis Data screen or when
the system is powered down. When “REMOTE STANDBY” or “SYSTEM

RUNNING” message is displayed on the screen, stop the BioTector using the
“Finish & Stop” or “Emergency Stop” function.

Caution

2.2 Operation Menu

Operation Menu Diagram

Operation menu allows the user to start and stop the analyzer. Menus related to system operation are also
accessed using this menu.

2.2.1 Start Stop

The user can Start or Stop the BioTector using the Start Stop menu.

1. Remote Standby. Remote Standby is an optional function, which is activated from Input 2 (by default) on

the Power and Input/Output Board (e.g. from a flow switch). A “REMOTE STANDBY” message is displayed
on the top left corner of the main Analysis Data screen to indicate that the BioTector is in remote standby
state. When remote standby signal is activated, the BioTector stops analyzing. All menu access and
operational functions remain as for BioTector normal running state. The BioTector runs one standby
reaction every 24 hours, at the time programmed for the Pressure/Flow Test (at 08:15 AM by default).
Sample is not taken during the remote standby reaction (only acid and base reagents are used). This
reaction is tagged as “RS” (Remote Standby) in the system reaction archive. The 4-20mA signal or other
output devices are not updated. When remote standby signal is deactivated, the BioTector starts analyzing.

When remote standby signal is activated, the “Finish & Stop” or “Emergency Stop” must be selected before

using such functions as Install New Reagents, Zero and Span Calibrations, Process Tests etc. If the

BioTector is stopped using the “Finish & Stop” or “Emergency Stop” functions or automatically by a system

fault, it will not be possible to start the BioTector by the removal of the remote standby signal. The “Start”
function must be used to re-start the BioTector. When BioTector is started while the remote standby signal
is activated, BioTector goes into remote standby state. The manual grab sample analysis can be carried out
normally using the Manual Program menu when the BioTector is in remote standby state.

Page 22

Quick Startup Function: During maintenance, system testing etc. it may be
necessary to quickly start and stop the BioTector to check various parameters.
Pressing the ENTER key for the “Start”, when the RIGHT ARROW key is also
pressed, bypasses the Pressure/Flow Test sequence, ensuring a quick startup.

When the quick startup function is used, system will log a “28_NO PRESSURE
TEST” warning in the fault archive and will start operation. The same warning will
also be logged, when the BioTector is started from the Reagents Setup, Manual
Program and Calibration menus using this function.

2. Start. This function starts the BioTector. When BioTector is started, the multi-stream operation sequence (if

programmed) is reset. BioTector performs Ozone Purge, Pressure/Flow Test, Reactor Purge and Analyzer
Purge sequences automatically before starting its analysis.

 Ozone Purge sequence purges any residual ozone through the ozone destructor.
 Pressure/Flow Test sequence confirm that there is no gas leak and there is no gas flow

restriction in the BioTector.

 Reactor Purge sequence purges any liquid from the reactor through the Reactor Valve.
 Analyzer Purge sequence purges any CO2 gas from the CO2 Analyzer through the Exhaust

Valve.

An “*” is displayed to let the operator know the function has been activated. If there is a fault in the system,

it will not be possible to start the analyzer until the fault has been rectified.

3. Finish & Stop. When this function is activated from the keyboard, the BioTector stops as soon as its

present reaction is completed. An “*” is displayed to let the operator know the function has been activated.

4. Emergency Stop. When this function is activated the BioTector cancels the execution of the present

reaction and quickly stops operation after the Ozone Purge, Reactor Purge and CO2 Analyzer Purge

sequences. An “*” is displayed to let the operator know the function has been activated. The Emergency

Stop has highest priority, and always overrules the “Finish & Stop” function.

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I N S T A L L N E W R E A G E N T S 0 9 : 1 7 : 2 8 1 2 - 0 9 - 0

2

C O N F I R M T H E F O L L O W I N G : 1 < N E W A C I D C O N N E C T E D 3 5 0 m g / l M n S O 4 . H 2 O 2 R E S E T A C I D M O N I T O R 2 5 . 0 l ~ 2 6 0 D A Y S 3 N E W B A S E C O N N E C T E D

4 R E S E T B A S E M O N I T O R 2 5 . 0 l ~ 2 6 0 D A Y

S

1 0 T O C 2 0 0 m g C , T I C 5 0 m g C C O N N E C T E D 1 0 S T A R T N E W R E A G E N T C Y C L E

N O T E : B I O T E C T O R W I L L S T O P W H E N T H E N E W R E A G E N T S C Y C L E I S C O M P L E T E

2.2.2 Reagents Setup

This menu allows the user to access the Reagent menus.

1. Install New Reagents. Menu used to install and prime the reagents in the BioTector. Any “85_Reagents
Low” and “20_No Reagents” warnings and notifications can also be reset in this menu.

2. Purge Reagents & Zero. Menu used to purge the reagents, and carryout a zero calibration cycle.

2.2.2.1 Install New Reagents

The install new reagents procedure is an automatic procedure for installing new reagents, setting the zero
offset by Zero Calibration cycle, setting the reaction check levels and checking the span by Span Calibration
or Span Check cycles. Span Calibration or Span Check cycles are part of the Install New reagents sequence
if SPAN CALIBRATION or SPAN CHECK is activated in New Reagents Program menu. The basic Zero
Check/Calibration and Span Check/Calibration parameters (operation ranges, number of reactions, standard
solution concentrations etc.) are programmed in Zero Calibration and Span Calibration menus respectively
(see Section 2.3 Calibration Menu for details). The comprehensive Zero Check/Calibration and Span
Check/Calibration parameters are programmed in Zero Program and Span Program menus respectively (see
Section 8.3.4.2 Zero Program and 8.3.4.3 Span Program for details).

To run the Install New Reagents cycle, the BioTector must be stopped. Confirm that all or the corresponding
new reagents have been installed on the BioTector, for instance for acid reagent, select New Acid Connected,
and press the ENTER key. A tick mark will appear as confirmation that the new acid has been connected.
Note that when one or more reagent volumes are updated in Reagents Monitor menu, system automatically
resets the new reagents volumes in this menu and also updates the figures displayed in the main Reagents
Status screen.

All reagents volumes can be reset while system is running. This function allows the user to top up the
reagents without stopping the system. However, when acid and/or base reagents are replaced or topped up,
system requires a new Zero Calibration cycle. A “ZERO CALIBRATION REQUIRED” warning will be
displayed on the screen when RESET ACID MONITOR and/or RESET BASE MONITOR are selected.
Therefore, it is strongly recommended to stop the BioTector and activate the Start New Reagent Cycle or to
run the Zero Calibration cycle using the Zero Calibration menu. Failure to do so may have an impact on
system zero response and the analysis results.

Page 24

When all or the necessary reagents have been confirmed to be connected and reset in this menu, and when
Start New Reagent Cycle is selected, the Install New Reagents cycle will be executed. It is the responsibility
of the user to make sure that all reagent volumes are programmed correctly in Reagents Monitor menu, the
reset of the reagents monitoring are carried out correctly in Install New Reagents menu and finally if
necessary the Zero Calibration cycle is activated either with the Start New Reagent Cycle function in Install
New Reagents menu or with the Run Zero Calibration function in Zero Calibration menu.

The Install New Reagents cycle consists of the following steps:

1. Reagent Purge: System purges and fills all reagent lines with the new reagents.

2. Zero Calibration: The Zero Adjust (zero offset) level is set for all analysis ranges, and the Reaction

Check level for TOC is updated (if the CO2 LEVEL is programmed as AUTO in Reaction Check
menu).

3. If Span Calibration or Span Check is activated in New Reagents Program menu, a Span Calibration

or Span Check is carried out.

Once the procedure is completed the BioTector either stops or returns online, depending on the programmed
setting of AUTOMATIC RE-START in New Reagents Program menu (see Section 8.2.5 New Reagents

Program for details).

2.2.2.2 Purge Reagents & Zero

The Purge Reagents & Zero function is an automatic procedure to purge the reagents, to set the zero offset
and to set the reaction check levels in the BioTector. The program settings for the Reagent Purge are set up
in the Reagents Purge menu.

1. Purge Reagents & Zero. This option allows the user to run the Purge Reagents & Zero cycle.

2.2.3 System Range Data Screen

This menu displays the system specific, factory calibrated, analysis range data for all measured components
(e.g. TIC, TOC, TC). BioTector can be calibrated with up to 3 analysis ranges for each measured component.
When a specific component of a sample (e.g. TOC) is measured at a specific range (e.g. Range 2), the
analysis of any other components (e.g. TIC etc.) of the sample are also carried out at the same analysis
range.

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M A N U A L P R O G R A M 0 9 : 1 7 : 2 8 1 2 - 0 9 - 0

2

1 < R U N A F T E R N E X T R E A C T I O N 2 R U N A F T E R 0 0 : 0 0 3 R E T U R N T O O N - L I N E S A M P L I N G Y E S

4 R E S E T M A N U A L P R O G R A M 5 6 M A N U A L 1 , 4 R A N G E 1 7 M A N U A L 2 , 4 R A N G E 3 8 M A N U A L 3 , 4 R A N G E 2 9 M A N U A L - , - - - R A N G E - 1 0 M A N U A L - , - - - R A N G E - 1 1 M A N U A L - , - - - R A N G E -

1 2 M A N U A L - , - - - R A N G E - 1 3 M A N U A L - , - - - R A N G E - ▼

2.2.4 Manual Program Menu

Manual Program menu allows the user to run system in manual operation mode in order to analyze grab
samples/standards or a sequence of samples/standards manually. This is achieved by one or a set of Manual
Valves installed in the system. The manual analysis sequence can be started at the end of the current
reaction, or at a time set by the user. When the manual sequence is complete, the system can be
programmed to return online automatically. Note that all pressure/flow tests, zero or span cycles are
interrupted by the manual operation mode. The Sample Pump reverse operation is also disabled during the
manual operation mode by default, unless a Manual Bypass Valve is installed in the system and the
REVERSE time is programmed for the corresponding Manual Valve in Sample Pump menu. All items in this
menu can be modified when the BioTector is running unless:

- No Manual Valves have been defined in the Output Devices menu.

- The manual mode is currently running.

- The manual mode is scheduled to start when the current reaction is completed.

Note that the Manual mode always starts at the first programmed valve, and works its way down the
programmed sequence.

1. Run After Next Reaction. To start the manual operation mode sequence after the next reaction the
BioTector is currently running, press the ENTER key at this menu item. An “*” will indicate that this
function has been selected. If the BioTector is stopped, then the Manual mode will start immediately. To
deactivate this function before the manual operation mode has started, press the ENTER key again, or
activate an alternative function. In systems built with the remote control of Manual Program option, the
remote signal (Manual Mode Trigger from Input 7) activates the Run After Next Reaction function.

2. Run After 00:00. Similar to menu option 1 above, but the manual operation mode starts after the
programmed time.

3. Return to On-line Sampling. This menu item allows the user to specify whether the BioTector should
stop (with NO setting) or return to online monitoring (with YES setting) when the manual operation
sequence is complete.

4. Reset Manual Program. Use this function to reset all the programmed settings to their default values.

6. - 30. Manual. In order to analyze one or a number of samples/standards using the manual operation mode,

first connect the sample/standard to the manual port/s outside the BioTector. Then, select the
corresponding Manual Valve in this menu (the first setting). Then, enter the number of samples (number
of analysis reactions) to be taken through each Manual Valve (the second setting). Finally select the
correct analysis range (RANGE 1, 2 or 3) if the concentration levels of the sample/standard are known.
See System Range Data screen (see Section 2.2.3 System Range Data Screen for details) to view the
available system ranges and to select the correct operation range. If the concentration levels of the
samples/standards are not known, select AUTO so that BioTector can automatically select the optimum
analysis range. When RANGE is programmed as AUTO, a minimum of five analysis reactions is
recommended (the second setting) so that BioTector can find the optimum operation range with its
automatic exceedance tracking function. When AUTO option is selected, depending on analysis range
and system response, the first two or three analysis results may need to be discarded.

Page 26

2.2.5 Reaction Archive Screen

The Reaction Archive holds information on TIC, TOC, TC, VOC, COD, BOD, stream valve, reaction range,
start time and related analysis information for the last 9999 reactions. If the archive is full, then every new
reaction overwrites the oldest one in the archive. As the Reaction Archive contains 9999 events, the user
must first enter the date at which the viewing of the archive starts. The Enter Date menu allows the user to
specify the date of the first displayed reaction from the archive.

Each reaction record in the reaction archive contains:

- Start Time - reaction start time, which is displayed without seconds in this menu

- Date - reaction date

- Reaction Type - with the prefixes below:

S1 to S3: Reactions from stream 1 to stream 3.
M1 to M3: Reactions from manual sample stream 1 to manual stream 3.

√ Sample Sensor detected the sample and the liquid quality is greater than the

threshold value for stream and manual samples.

x Sample Sensor detected no sample or the liquid quality is less than the

threshold value for stream and manual samples. See Sample Status in

section 8.3.8 Fault Setup for details.
RS: Remote standby reaction.
ZC: Zero calibration reaction.
ZK: Zero check reaction.
ZM: Manually input zero adjust.
SC: Span calibration reaction.
SK: Span check reaction.
SM: Manually input span adjust.
A1 to A3: 24 hours average result from stream 1 to stream 3.

The user can navigate through the displayed reactions individually by pressing the UP and DOWN keys each
time, or can navigate in steps of 10 reactions using the LEFT and RIGHT keys. Depending on system
analysis type (e.g. VOC, TC –TIC etc.) and system display options (e.g. COD and/or BOD) settings, BioTector
displays additional reaction data held on additional Reaction Archive screens. To access the screens, press
the ENTER key, and to return to the previous screen, press the ESCAPE key.

2.2.6 Fault Archive Menu

In the Fault Archive menu, the user can view the last 99 faults/warning/notification events logged in the
system, confirm if these events are current or not, and acknowledge the current events. If the archive is full,
then every new event overwrites the oldest one in the archive. The user can navigate through the displayed
reactions individually by pressing the UP and DOWN keys each time, or can navigate in steps of 10 reactions
using the LEFT and RIGHT keys. See Section 9 Troubleshooting of System Fault, Warning and

Notification Events for a list of all systems fault, warning and notification events.

The faults archive events are divided into three categories:

- Fault: Faults are categorized as events, which stop BioTector operation. The 4-20mA signals are set
to the fault level, and the fault relay is activated. The BioTector cannot be started unless the fault in
the archive has been acknowledged.

- Warning: Warning is a minor event, which does not require the BioTector to stop. The 4-20mA
signals are not changed, only the fault relay is activated.

- Notification: A notification is an information (e.g. “86_Power Up”, “87_Service Time Reset” etc.)
displayed on the screen.

To acknowledge any current events marked with an “*” in the archive, first identify and locate the
faults/warnings/notification. Follow the necessary troubleshooting procedures to solve the problem. See

Section 9 Troubleshooting of System Fault, Warning and Notification Events for details.

Acknowledge the fault by pressing the ENTER key in the Fault Archive menu. Please note that there are
system faults (e.g. 05_Pressure Test Fail), which cannot be acknowledged by the user. Such faults are reset
and acknowledged automatically by the system when system is started, when system is rebooted or when the
fault condition is solved. If an event cannot be acknowledged when the system is running, a “SYSTEM
RUNNING” message is displayed on screen.

Page 27

When the time is changed, it is possible for the BioTector to automatically start up if the
new time is after the startup time for a scheduled task, for example the startup time for
a manual sample sequence in Manual Program menu.

2.2.7 Time & Date Menu

This menu allows the system time and date to be set by the user. To change the system time or date (hours,
minutes, seconds, day, month and year), press the ENTER key, enter the new time and date and press the
ENTER key again.

In order to change the system date format, press the ENTER key, select new date format from the following
day, month and year options: DD-MM-YY, MM-DD-YY, YY-MM-DD and press the ENTER key again.

2.2.8 Contact Information

Contact Information menu displays the manufacturer/distributor contact details.

2.2.9 LCD Adjust

LCD Adjust menu allows the user to adjust the LCD screen’s contrast and backlight level. The default contrast
and backlight setting is 50%. The adjustment is carried out by entering to the relevant setting and making the
necessary change using the UP or DOWN arrow keys. When the UP/DOWN key is pressed and kept
pressed, BioTector displays the relevant adjustment in real time. When the adjustment is completed, press
the ENTER key to accept the new setting or press ESCAPE key to return back to the previous setting.

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Z E R O C A L I B R A T I O N 0 9 : 1 7 : 2 8 1 2 - 0 9 - 0

2

1 < Z E R O A D J U S T 1 0 . 0 [ 0 . 0 ] 2 2 0 . 0 [ 0 . 0 ] 3 3 0 . 0 [ 0 . 0 ] 4 R U N R E A G E N T S P U R G E 5 R U N Z E R O C A L I B R A T I O N 6 R U N Z E R O C H E C K

7 R 1 R 2 R 3 8 Z E R O P R O G R A M 6 , 4 , 4 9 Z E R O A V E R A G E 4 , 2 , 2 1 0

1 1 - - > Z E R O P R O G R A M

ENTER PASSWORD CALIBRATION ZERO CALIBRATION

SPAN CALIBRATION

2.3 Calibration Menu

Calibration menu allows the user to calibrate the analyzer. Zero and Span Calibration menus allow the user to
run the zero and span calibration cycles for a single range or for all system ranges available.

Calibration Menu Diagram

2.3.1 Zero Calibration

Zero Calibration menu allows the user to enter the suggested Zero Adjust values, to start the Reagent Purge
cycle, to start the Zero Calibration and Zero Check cycles and to program the number of zero reactions run at
each range.

1.-3. Zero Adjust. The Zero Adjust is used to compensate any organic contamination in the acid and base
reagents and any absorbed CO2 in the base reagent. The Zero Adjust values are generated automatically
by the system for each range when the zero calibration cycle is completed without any system warnings.
Zero Calibration cycle is activated by selecting the RUN ZERO CALIBRATION function in this menu.

When a Zero Check cycle is run using the RUN ZERO CHECK function, the system only checks the zero

response at each range and displays the suggested Zero Adjust values in brackets “[ ]” for all ranges next

to the current Zero Adjust settings. When a Zero Check cycle is completed, if necessary the suggested
Zero Adjust values can be programmed manually by entering the corresponding suggested Zero Offset
values for each range (1, 2 and 3) in this menu. When the Zero Adjust settings are entered manually,
system logs this information in the reaction archive with the prefix “ZM” (Zero Manual).

4. Run Reagents Purge. The RUN REAGENTS PURGE function is used to prime all reagents in the
BioTector. If necessary, the pump operation time for Reagent Purge cycle can be increased in the
Reagents Purge menu.

5. Run Zero Calibration. Each time BioTector reagents are replaced or topped up and each time a service
is carried out, it is strongly recommended to use the RUN ZERO CALIBRATION function so that the
system can set the zero offset values automatically. The zero calibration reactions operate in the same
manner as a normal reaction, but BioTector does not take any sample. To start the zero calibration, press

the ENTER key at this menu item. An “*” will indicate that the function is running. At the end of the Zero

Calibration cycle, the following settings are checked and updated:

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1. The Zero Adjust settings for each range are updated automatically by the system using the un­calibrated TOC measurement (not the results seen on the LCD screen). If a Zero Check is used
to check the zero offset, the suggested values are shown in brackets “[ ]” next to the actual Zero
Adjust settings.

2. If the CO2 LEVEL is set as AUTO for automatic updating in the Reaction Check menu, then the
reaction check CO2 Level is also updated automatically.

3. The CO2 Level is also checked against the BASE CO2 ALARM setting in Fault Setup menu. If the
measured CO2 Level is greater that the BASE CO2 ALARM value, system generates a “52_HIGH
CO2 IN BASE” warning.

6. Run Zero Check. Zero Check cycle is similar to the Zero Calibration above, but BioTector does not
update any of the Zero Adjust or CO2 Level settings. System only checks the BASE CO2 ALARM
described above.

8. Zero Program. Zero Program function allows the user to program the number of zero reactions run at
one or more ranges (R1, R2 and/or R3). When the number of zero calibration reactions for one or two of
the ranges is set to zero, system runs the zero cycle on the programmed range or ranges and calculates
the Zero Adjust values for the other ranges automatically. It is recommended not to modify the factory set
Zero Program values unless it is absolutely necessary. Any unnecessary modification in this setting may
have an impact on the zero offset values.

9. Zero Average. Zero Average function allows the user to program the number of zero reactions to be
averaged for each range (R1, R2 and/or R3) at the end of the zero cycles. It is recommended not to
modify the factory set Zero Average values unless it is absolutely necessary. Any unnecessary
modification in this setting may have an impact on the zero offset values.

11.  Zero Program. Zero Program is a link to Maintenance, System Configuration, Sequence Program,
Zero Program menu. See Section 8.3.4.2 Zero Program.

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