Hach Pocket Colorimeter II, 59530-01, 59530-51, 59530-12 Instruction Manuals

59571-88

POCKET COLORIMETER™ II

ANALYSIS SYSTEMS

INSTRUCTION MANUAL

Bromine (Br)

Chlorine Dioxide (ClO

2

)

Chlorine (Cl

2

) / pH

© Hach Company, 2004–2006. All rights reserved. Printed in the U.S.A. te/dk 9/06 4ed

1—2

Important Note

This manual is intended for use with the following Pocket Colorimet er™ II
instruments:

The Pocket Colorimeter™ II instruments listed above are not interchangeable.

Bromine Cat. No. 59530-01
Chlorine Dioxide Cat. No. 59530-51
Chlorine / pH Cat. No. 59530-12

1—3

Table of Contents

Safety Precautions............................................................................................... 1—7

Laboratory Safety............................................................................................... 1—7

Use of Hazard Information................................................................................ 1—7

Precautionary Labels........ ....... ....... ....................................................................1— 8

Specifications......................................................................................................... 1—9

Instrument Keys and Display...........................................................................1—11

Instrument Cap Cord.........................................................................................1—12

Bromine, Total, LR..............................................................................................1—15

Measuring Hints ...............................................................................................1—15

Sampling and Storage......................................................................................1—22

Accuracy Check................ ....... .............................................................. ...........1—22

Interferences......................................................................................................1—24

Method Performance........................................................................................1—26

Bromine, Total, HR.............................................................................................1—27

Measuring Hints ...............................................................................................1—27

Sampling and Storage......................................................................................1—31

Accuracy Check................ ....... .............................................................. ...........1—31

Interferences......................................................................................................1—33

1—4

Table of Contents, continued

Method Performance........................................................................................1—35

Summary of Method.........................................................................................1—35

Replacement Parts (LR and HR)......................................................................1—35

Chlorine, Free and Total, HR..........................................................................1—39

Measuring Hints................................................................................................1—39

Accuracy Check............................................. ....................................................1—44

Interferences......................................................................................................1—46

Spec

√ Secondary Standards............................................................................1—49

Method Performance........................................................................................1—49

Summary of Method.........................................................................................1—49

Replacement Parts.............................................................................................1—50

Chlorine Dioxide..................................................................................................1—53

Measuring Hints................................................................................................1—53

Sampling and Storage......................................................................................1—61

Accuracy Check............................................. ....................................................1—61

Method Performance........................................................................................1—64

Interferences......................................................................................................1—64

Summary of Method.........................................................................................1—66

Reagents and Apparatus................................................................ ....... ...........1—6 6

1—5

Table of Contents, continued

pH..............................................................................................................................1—69

Measuring Hints ...............................................................................................1—69

Accuracy Check................ ....... .............................................................. ...........1—74

Method Performance........................................................................................1—74

Standard Calibration Adjust ...........................................................................1—75

Summary of Method........................................................................................1—75

Replacement Parts............................................................................................1—76

Instrument Operation.........................................................................................2—3

Key Functions..................................................................................................... 2—3

Menu Selections.............................. ....................................................................2— 4

Switching Ranges............................................................................................... 2—4

Setting the Time ................................................................................................. 2—4

Recalling Stored Measurements........................................................................ 2—5

Battery Installation............................................................................................. 2—6

Error Codes.............................................................................................................. 2—9

Error Messages.................................................................................................... 2—9

Standard Calibration Adjust..........................................................................2—13

1—6

Table of Contents, continued

User-Entered Calibration.................................................................................2—15

Overview............................................................................................................2—15

Calibration Procedure Using Prepared Standards.........................................2—17

Entering a Predetermined Calibration Curve.......... .......................................2—20

Editing a User-entered or Factory Calibration Curve...................................2—22

Exiting the Calibration Routine......................... .............................................2—24

Deleting Calibration Points.......................... ....... ...... .......................................2—24

Retrieving the Factory Calibration ............................................... ..................2 —25

Maximum/Minimum Displayed Value....................................................... ....2—26

Certification..........................................................................................................2—28

How to Order........................................................................................................2—35

Repair Service.......................................................................................................2—37

Warranty.................................................................................................................2—38

1—7

Safety Precautions

Please read this entire manual before unpacking, setting up, or operating this
instrument. Pay particular attention to all danger and caution statements. Failure
to do so could result in serious injury to the operator or damage to the equipment.

To ensure the protection provided by this equipment is not impaired, do not use or
install this equipment in any manner other than that which is specified in
this manual.

Laboratory Safety

As part of good laboratory practice, pl eas e familiarize yourself with the reagents
used in these procedures. Read all product labels and the m aterial safety data
sheets (MSDS) before using them. It is always good practice to wear safety glasses
when handling chemicals. Foll ow instructions carefully. Rinse thoroughly if
contact occurs. If you have questions about reagents or procedures, please contact
the manufacturer or distributor.

Use of Hazard Information

If multiple hazards exist, this manu al wi ll use th e sign al wo rd (Danger, Caution,
Note) corresponding to the greatest hazard.

1—8

Safety Precautions, continued

DANGER
Indicates a potentially or imminently hazardous situation which, if not
avoided, could result in death or serious injury.

CAUTION
Indicates a potentially hazardous situation that may result in minor or
moderate injury.

NOTE
Information that requires special emphasis.

Precautionary Labels

Please pay particular attention to labels and tags attached to the instrument.
Personal injury or damage to the instrument could occur if not observed.

This symbol, if noted on the instrume n t, referen ces the instruct io n manual

for operational and/or safety inform ation.

1—9

Specifications

Lamp: Light emitting diode (LED)
Detector: Silicon photodiode
Photometric precision: ± 0.0015 Abs
Filter bandwidth: 15 nm
Wavelength: 528 nm
Absorbance range: 0–2.5 Abs
Dimensions: 3.2 x 6.1 x 15.2 cm (1.25 x 2.4 x 6 inches)
Weight: 0.2 kg (0.43 lb)
Sample cells: 25 mm (10 mL), 1 cm (10 mL), AccuVac

®

Ampuls

Operating conditions: 0 to 50 °C (32 to 122 °F); 0 to 90% relative humidity
(noncondensing)

Power supply: Four AAA alkaline batteries; approximate life is 2000 tests*

* Backlight usage will decrease battery life.

1—10

OPERATION

DANGER

Handling chemical samples, standards, and reagents can be dangerous. Review the necessary
Material Safety Data Sheets and become familiar with all safety procedures before handling
any chemicals.

DANGER

La manipulation des échantillons chimiques, étalons et réactifs peut être danger euse. Lir e les Fiches
de Données de Sécurité des Produits (FDSP) et se familiariser avec toutes les procédures de sécurité
avant de manipuler tous les produits chimiques.

PELIGRO

La manipulación de muestras químicas, estándares y reactivos puede ser peligro s a. Revi se las fichas
de seguridad de materiales y familiarícese con los procedimientos de seguridad antes de manipular
productos químicos.

GEFAHR

Das Arbeiten mit chemischen Proben, Standards und Reagenzien ist mit Gefahren verbunden. Es
wird dem Benutzer dieser Produkte empfohlen, sich vor der Arbeit mit sicheren Verfahrensweisen
und dem richtigen Gebrauch der Chemikalien vertraut zu machen und alle entsprechenden
Materialsicherheitsdatenblätter aufmerksam zu lesen.

PERIGO

A manipulação de amostras, padrões e reagentes químicos pode ser perigosa. Reveja a folha dos
dados de segurança do material e familiarize-se com todos os procedimentos de segurança antes
de manipular quaisquer produtos químicos.

PERICOLO

La manipolazione di campioni, standard e reattivi chimici può essere pericolosa. La preghiamo di
prendere conoscenza delle Schede Techniche necessarie legate alla Sicurezza dei Materiali e di
abituarsi con tutte le procedure di sicurezza prima di manip olare ogni prodotto chimico.

1—11

Instrument Keys and Display

Item Description

1 POWER/BACKLIGHT Key
2

ZERO/SCROLL Key

3

MENU Key

4 Numeric Display
5 Range Indicator
6 Range Indicator
7 Menu Indicator
8 Calibration Adjusted Indicator
9 Battery Low Indicator

10

READ/ENTER Key

1—12

Instrument Cap Cord

The instrument cap for the Pocket Colorimeter™ II doubles as a light shield.
Accurate measurements cannot be obtained unless the sample or blan k is covered
with the cap. Use the instrument cap cord to secure the cap to the body of the
colorimeter and prevent loss of the cap. See Figu re 1 on page 1—13.

1. Loop the instrument cap cord through the ring on the cap.

2. Remove the battery compartment cover. Press the knotted end of the cord into

the hole indicated by the arro w.

3. Slide the cord into the slot on the battery compartment cover. Snap the cover
into place.

1—13

Instrument Cap Cord, continued

Figure 1 Attaching the Instrument Cap Cord

1—14

1—15

Bromine, Total, LR (0.05 to 4.50 mg/L Br

2

)

Method 8016

For water, wastewater, and seawater

DPD Method* (PermaChem® Powder Pillows or AccuVac® Ampuls)

Measuring Hints

• Analyze samples immediately . Do not use plastic containers to collect sample s.

Note:

The Pocket Colorimeter™ II is designed to measure solutions contained in sample cells.

DO NOT

dip the meter in the sample or pour the sample directly into the cell holder.

Note:

If the sample temporarily turns yellow after reagent addition, or if the display shows
overrange (page 2—10), dilute a fresh sample and repeat the test. A slight loss of bromine
may occur because of the dilution. Multiply the result by the appropriate dilution factor.

* Adapted from Standard Methods for Examination of Water and Wastewater.

1—16

Bromine, Total, LR, continued

Bromine, Total, Low Range (using Powder Pillows)

1.Fill a 10-mL sample cell

sample (the blank). Cap.

Note:

Samples must be
analyzed immediately and
cannot be preserved for later
analysis.

2.Pr ess the POWER key to

turn the meter on.
The arrow sh ould indicate

the low range channel (LR).

Note:

See page 2—4 for
information on selecting the
correct range channel.

3.Remove the instrument

cap. Place the blank in the
cell holder. Cover the blank
with the instrument cap.

Note:

Wipe excess liquid and
fingerprints from the sample
cells.

1—17

Bromine, Total, LR, continued

4.Press ZERO/SCROLL.

The display will show
“- - - -” then “0.00”.

Remove the blank fro m the
cell holder.

5.Fill a second sample cell

to the 10 mL line with
sample. Wipe off any liquid
or fingerprints.

6.Ad d the cont ents of one

DPD Total Chlorine Powder
Pillow to the sample cell (the
prepared sample).

Note:

The SwifTest™
Dispenser for total chlorine
can be used in place of the
powder pillow.

1—18

Bromine, Total, LR, continued

7.Cap and s hake gently for

20 seconds.

Note:

Shaking dissipates
bubbles that may form in
samples with dissolved gases.

Note:

A pink color will
develop if bromine is present.

Note:

Wipe liquid off sample

cells.

8.Place the prepared

sample in the cell holder and
cover with the instrument
cap. Wait at least three but
no more than six minutes
after adding the DPD Total
Chlorine Pillow.

Note:

Accuracy is not
affected by undissolved
powder.

9.Press READ/ENTER.

The display will show
“- - - -”, followed by results
in mg/L bromine.

1—19

Bromine, Total, LR, continued

Bromine, Total, Low Range (using AccuVac® Ampuls)

1.Fill a 10-mL sample

cell with sample (the
blank). Cap. Collect at
least 40 mL of sample in
a 50-mL beaker.

Note:

Samples must be
analyzed immediately and
cannot be preserved for
later analysis.

2.Press the POWER key

to turn the meter on.
The arrow should indicate

the low range channel
(LR).

Note:

See page 2—4 for
information on selecting
the correct range channel.

3.Remove the instrument

cap. Place the blank in the
cell holder. Cover the
blank with the instrument
cap.

Note:

Wipe excess liquid
and fingerprints from the
sample cells.

1—20

Bromine, Total, LR, continued

4.Press ZERO/SCROLL.

The display will show
“- - - -” then “0.00”.

Remove the blank from
the cell holder.

5.Fill a DPD Total

Chlorine Reagent AccuV ac
Ampul with sample. Keep
the tip of the ampule
immersed until the ampule
fills completely.

6.Quickly invert the

ampul several times to
mix. Wipe off any liquid or
fingerprints.

Note:

A pink color will

form if bromine is present.

Note:

Accuracy is not
affected by undissolved
powder.

1—21

Bromine, Total, LR, continued

7.Place the prepared

sample in the cell holder
and cover with the
instrument cap. Wait at
least three but no more
than six minutes after
filling the ampule.

Note:

Wipe liquid off

sample cells.

8.Press READ/ENTER.

The display will show
“- - - -”, followed by
results in mg/L bromine.

1—22

Bromine, Total, LR, continued
Sampling and Storage

Analyze samples immediately. Do not collect samples in plasti c con tai ners.

Accuracy Check

Standard Additions Method

1. Snap the neck off a low range Chlorine Standard Solution PourRite® Ampule,
20–30 mg/L Cl

2

.

2. Use a TenSette

®

pipet to add 0.1, 0.2, and 0.3 mL of standard to three 10-mL
samples. Swirl gently to mix. (For AccuVac Ampuls, use 0.3, 0.6, and 0.9 mL
of standard and a 30-mL sample in a 50-mL beaker.)

3. Analyze each sample as described in the procedure. Each 0.1 mL of stan da rd
will cause an incremental increase in the apparent bromine concentration. The
exact value depends on the concentration of the ampule standard. Check the
certificate enclosed with the standard ampules for the chlorine concentration.
Multiply the expected chlorine concentration by 2.25 to determine the
expected increase in bromine concentration.

1—23

Bromine, Total, LR, continued

Standard Solution Method

Standard solutions for bromine or chlorine are difficult and time-consuming to
prepare. Errors can occur if attention to detail is not addressed during preparation
of the standards. Hach Company prepares the calibration curve under rigorous
analytical laboratory conditions. Hach strongly recommends using the facto ry
calibration.

A user calibration or a user-prepared br omine standard may be required by a
regulatory official or agency. Two options are available on the Pocket
Colorimeter™ II to meet this requirement.

A bromine standard may be prepared and used to validate th e calib ration curve
using the Standard Calibration Adjust feature (see page 2—13 for more
information.). The concentration of the prepared standard must be determined with
an alternate instrument such as a spectrophotometer, colorimeter, amperometric
titration, or by using another alternate method. The concentration of the bromine
standard for the low range procedure must be between 2.00 and 4.00 mg/L
bromine.

In addition, a user-generated calibration curve can be made and programmed into
the Pocket Colorimeter™ II. See User-Entered Calibration on page 2—15.

1—24

Bromine, Total, LR, continued

If a chlorine standard is used, multiply its concentration by 2.25 to determine the
equivalent bromine concentration.

Interferences

Interfering
Substance

Interference Levels and Treatments

Acidity Greater than 150 mg/L CaCO3. May not develop full color or

color may fade instantly. Neutralize to pH 6–7 with 1 N Sodium
Hydroxide. Determine amount to be added on separate 10-mL
sample, then add the same amount to the sample being tested.
Correct for the additional volume.

Alkalinity Greater than 250 mg/L CaCO

3

. May not develop full color or
color may fade instantly. Neutralize to pH 6–7 with 1 N Sulfuric
Acid. Determine amount to be added on separate 10-mL sample,
then add the same amount to the sample being tested. Correct
for the additional volume.

Chlorine, Cl

2

Interferes at all levels

1—25

Bromine, Total, LR, continued

Hardness No effect at less than 1,000 mg/L as CaCO

3

Iodine, I

2

Interferes at all levels

Manganese,
Oxidized
(Mn

4+

, Mn7+) or
Chromium,
Oxidized (Cr

6+

)

1. Adjust sample pH to 6–7.

2. Add 3 drops Potassium Iodide (30-g/L) (Cat. No. 343-32)

to a 25-mL sample.

3. Mix and wait one minute.

4. Add 3 drops Sodium Arsenite (5-g/L) (Cat. No. 1047-32)

and mix.

5. Analyze 10 mL of the treated sample as described in

the procedure.

6. Subtract the result of this test from the original analysis

result to obtain the correct chlorine concentration.
Monochloramine Interferes at all levels.
Ozone Interferes at all levels.

Interfering
Substance

Interference Levels and Treatments

1—26

Bromine, Total, LR, continued
Method Performance

Estimated Detection Limit (EDL) = 0.05 mg/L Br

2

Typical Precision (95% confidence interval) = 2.25 ± 0.11 mg/L Br

2

1—27

Bromine, Total, HR (0.2 to 10.0 mg/L Br

2

)

Method 8016

For water, wastewater, and seawater

DPD Method* (PermaChem® Powder Pillows)

Measuring Hints

• If the bromine concentration is typically less than 4 mg/L, use the low range
procedure.

• Analyze samples immediately . Do not use plastic containers to collect sample s.

Note:

The Pocket Colorimeter II is designed to measure solutions contained in sample cells.

DO NOT

dip the meter in the sample or pour the sample directly into the cell holder.

* Adapted from Standard Methods for Examination of Water and Wastewater.

1—28

Bromine, Total, HR, continued

1.Fill a 1-cm/10-mL

sample cell with sample.
Cap. This is the blank.

Note:

Samples must be
analyzed immediately and
cannot be preserved for later
analysis.

2.Pr ess the POWER key to

turn the meter on.
The arrow sh ould indicate

the high range channel (HR).

Note:

See page 2—4 for
information on selecting the
correct range channel.

3.Remove the meter cap.

Place the blank into the cell
holder, with the diamond
mark facing the back of the
cell holder. Cover the cell
with the cap.

Note:

Wipe excess liquid and
fingerprints from the sample
cells.

1—29

Bromine, Total, HR, continued

4.Press ZERO/SCROLL.

The display will show
“- - - -” then “0.0”.

Remove the blank fro m the
cell holder.

5.Fill another 1-cm/1 0-mL

sample cell to the 5-mL line
with sample.

6.Add the contents of two

DPD Total Chlorine Powder
Pillows to the sample cell
(the prepare d sample).

Note:

The SwifTest™
Dispenser for Total Chlorine
can be used in place of the
powder pillows. Use two
dispensations from the
dispenser.

1—30

Bromine, Total, HR, continued

7.Cap and s hake gently for

20 seconds.

Note:

Shaking dissipates
bubbles that may form in
samples with dissolved gases.

Note:

A pink color will
develop if bromine is present.

Note:

Wipe liquid off sample

cells.

8.Place the prepared

sample in the cell holder and
cover with the instrument
cap. Wait at least three but
no more than six minutes
after adding the DPD Total
Chlorine Pillows.

Note:

Accuracy is not
affected by undissolved
powder.

9.Press READ/ENTER.

The display will show
“- - - -”, followed by results
in mg/L bromine.

10

m

L

5

m

L

1—31

Bromine, Total, HR, continued

Note:

If the sample temporarily turns yellow after reagent addition, or if the display shows
overrange (page 2—12), dilute a fresh sample and repeat the test. A slight loss of bromine
may occur because of the dilution. Multiply the result by the appropriate dilution factor.

Sampling and Storage

Analyze samples immediately. Do not collect samples in plasti c con tai ners.

Accuracy Check

Standard Additions Method

1. Snap the neck off a high range Chlorine Standard Solution V oluette® Ampule,
25–30 mg/L Cl

2

.

2. Use a TenSette

®

pipet to add 0.1, 0.2, and 0.3 mL of standard to three separate

5-mL samples. Swirl gently to mix.

3. Analyze 5 mL of each sample as described in the procedure. Each 0.1 mL of
standard will cause an incremental in crease in bromine concentration. The
exact value depends on the concentration of the ampule standard. Check the
certificate enclosed with the standard ampules for the chlorine concentration.
Multiply the expected chlorine concentration by 2.25 to determine the
expected increase in bromine concentration.

1—32

Bromine, Total, HR, continued

Standard Solution Method

Standard solutions for bromine or chlorine are difficult and time-consuming to prepare.
Errors can occur if attention to detail is not addressed during preparation of the
standards. Hach Company prepares the calibration curve under rigorous analytical
laboratory conditions. Hach stron gly recomm en ds usin g th e factory calibration.

A user calibration or a user-prepared br omine standard may be required by a regulatory
official or agency. Two options are available on the Pocket Colorimeter II to meet this
requirement.

A bromine standard may be prepared and used to validate the calibration curve using
the Standard Calibration Adjust feature (see page 2—13 for more information.). The
concentration of the prepared standard must be determined with an alternate
instrument such as a spectro ph ot om et er, colorimeter, amperometric titration, or by
using another alternate method. The concentration of the bromine standard for the low
range procedure must be between 2.00 and 4.00 mg/L bromine and between 2.0 and 8.0
mg/L bromine for the high range procedure.

In addition, a user-generated calibration curve can be made and programmed into the
Pocket Colorimeter™ II. See User-Entered Calib rati on on pa ge 2—1 5.

1—33

Bromine, Total, HR, continued

If a chlorine standard is used, multiply its concentration by 2.25 to determine the
equivalent bromine concentration.

Interferences

Interfering
Substance

Interference Levels and Treatments

Acidity Greater than 150 mg/L CaCO3. May not develop full color or

color may fade instantly. Neutralize to pH 6–7 with 1 N Sodium
Hydroxide. Determine amount to be added on separate 10-mL
sample, then add the same amount to the sample being tested.
Correct for the additional volume.

Alkalinity Greater than 250 mg/L CaCO

3

. May not develop full color or
color may fade instantly. Neutralize to pH 6–7 with 1 N Sulfuric
Acid. Determine amount to be added on separate 10-mL sample,
then add the same amount to the sample being tested. Correct
for the additional volume.

Chlorine, Cl

2

Interferes at all levels

1—34

Bromine, Total, HR, continued

Hardness No effect at less than 1,000 mg/L as CaCO

3

Iodine, I

2

Interferes at all levels

Manganese,
Oxidized
(Mn

4+

, Mn7+) or
Chromium,
Oxidized (Cr

6+

)

1. Adjust sample pH to 6–7.

2. Add 3 drops Potassium Iodide (30-g/L) (Cat. No. 343-32) to

a 25-mL sample.

3. Mix and wait one minute.

4. Add 3 drops Sodium Arsenite (5-g/L) (Cat. No. 1047-32)

and mix.

5. Analyze 10 mL of the treated sample as described in

the procedure.

6. Subtract the result of this test from the original analysis

result to obtain the correct chlorine concentration.
Monochloramine Interferes at all levels.
Ozone Interferes at all levels.

Interfering
Substance

Interference Levels and Treatments

1—35

Bromine, Total, HR, continued

Method Performance

Estimated Detection Limit (E DL) = 0.2 m g/L Br

2

Typical Precision (95% confidence interva l) = 11.3 ± 0.5 mg/L Br

2

Summary of Method

Bromine residuals react with DPD (N,N-diethy l-p-p he nylenediamine) to form a
pink color which is proporti onal to the tota l bromine concent ration. The rang e of
analysis is extended in the high range procedure by adding additional powder to
increase the ratio of indicator to sample.

Replacement Parts (LR and HR)

Required Reagents

Description Unit Cat. No.

DPD Total Chlorine Reagent Po wder Pillows............................100/pkg.....21056-69

or

DPD Total Chlorine Reagent AccuVac

®

Ampuls..........................25/pkg.....25030-25

1—36

Bromine, Total, HR, continued

Required Apparatus (Using AccuVac® Ampuls)

Description Unit Cat. No.

Beaker, 50 mL ......................................................................................each ......500-41H

Optional Reagents

Chlorine Standard Solution, Voluette® Ampules,

50-75 mg/L, 10 mL..................................................................... 16/pkg ....14268-10

Chlorine Standard Solution, Pour Rite Ampules,

25-30 mg/L.................................................................................. 20/pkg ....26300-20

Potassium Iodide Solution, 30 g/L.....................................100 mL MDB........ 343-32

Sodium Arsenite Solution, 5 g/L........................................100 mL MDB.......1047-32

Sodium Hydroxide Standard Solution, 1 N......................100 mL MDB .......1045-32

Sulfuric Acid Standard Solution, 1 N................................100 mL MDB .......1270-32

SwifTest™ Dispenser with DPD Total Reagent.................................each ....28024-00

Water, deionized....................................................................................4 L ........ 272-56

Optional Apparatus

AccuVac® Snapper Kit.......................................... ....... ........................each ....24 05 2-0 0

Caps for 10-mL sample cells............................................ ............. 12/pkg .... 24018-12

Caps for 1-cm/10-mL sample cells............................ ...... ....... ...........each ....5262 6-0 0

1—37

Bromine, Total, HR, continued

Optional Apparatus, continued

Description Unit Cat. No.

Cylinder, graduated, 25 mL, poly........................................... ....... ....each.......1081-40

Cylinder, graduated, 100 mL, PMP........................................ ...........each.......2172-42

sens

ion™1 Basic Portable pH Meter, with electrode.....................each..... 51700-10

Pipet, TenSette

®

, 0.1 to 1.0 mL..........................................................each.....19700-01

Pipet Tips, For 19700-01 TenSette

®

..............................................50/pkg.....21856-96

Sample Cells, 10-mL with screw caps ............................................6/pkg.....24276-06

Replacement Parts

Batteries, AAA, alkaline...................................................................4/pkg.....46743-00

Instrument Cap/light shield............................................. ....... ....... ....each.....59548-00

Instruction Manual.............................................................................each.....59571-88

Sample Cells, 1-cm/10-mL........................... ....................................2/pkg.....41658-02

1—38

1—39

Chlorine, Free and T otal, HR (0.1 to 10.0 mg/L Cl

2

)

Method 10069 (Free)

For water, treated water, estuary water, and seawater

Method 10070 (Total)

For water, treated waters, wastewater, estuary water, and seawater

DPD Method*

USEPA accepted for reporting drinking water analyses (free and total chlorine)
and wastewater analyses (total chlorine)

Measuring Hints

• If the chlorine concentration is typically less than 2 mg/L, use the Chlorine Pocket
Colorimeter (Cat. No. 58700-00).

• Analyze samples immediately. Do not use plastic containers to collect samples.
For best results, dedicate a set of cells to each test (free and total).

* Adapted from Standard Methods for the Examination of Water and Wastewater.

1—40

Chlorine, Free and Total, HR, continued

• High range free chlorine determinations are subject to variable levels of
interferences from monochloramine. See Interferences on page 1—46.

Note: The Pocket Colo rimeter™ II is designed to measure solutions contained in sample cells.

DO NOT dip the meter in the sample or pour the sample directly into the cell holder.

1—41

Chlorine, Free and T otal, HR, continued

1.Fill a 1-cm/10 -mL cell to

the 5-mL line with sample
(the blank). Cap.

Note: Samples must be

analyzed immediately and
cannot be preserved for
later analysis.

2.Press the POWER key to

turn the meter on.
The arrow should indicate

the chlorine channel
(mg/L Cl

2

).

Note: See page 2—4 for

information on selecting the
correct range channel.

3.Remove the meter cap.

Place the blank into the cell
holder, with the diamond
mark facing the back of the
cell holder. Cover the cell
with the cap.

Note: Wipe liq uid off sample

cells.

1—42

Chlorine, Free and Total, HR, continued

4.Press: ZERO/SCROLL

The display will show
“- - - -” followed by “0.0”.

Remove the blank.

5.Fill another 1-cm/10-mL

sample cell to the 5-mL line
with sample. Cap.

Note: Do not use the same

sample cells for free and
total chlorine without
thoroughly rinsing the cells
between the free and total
tests.

6.Add the contents of one

DPD Free Chlorine or one
DPD Total Chlorine Powder
Pillows to the sample cell
(the prepared sample). Cap
the cell and shake gently for
20 seconds.

Note: Wipe liq uid off sample

cells.

1—43

Chlorine, Free and T otal, HR, continued

7.For free chlorine, place

the prepared sample cell in
the cell holder within one
minute after adding the DPD
Free Pillow. Cover the cell
with the instrument cap.
Proceed to step 9.

8.For total chlorine, plac e

the prepared sample in the
cell holder and cover with
the instrument cap. Wait
three to six minutes after
adding the DPD Total
Pillows.Proceed to step 9.

9.Press READ/ENTER.

The instrument will show
“- - - -” followed by the
results in mg/L chlorine
(Cl

2

).

Note: If the sample temporarily turns yellow after reagent addition or shows overrange

(page 2—12), dilute a fresh sample and repeat the test. A slight loss of chlorine may occur.
Multiply the result by the dilution factor.

1—44

Chlorine, Free and Total, HR, continued
Accuracy Check

Standard Additions Method

a. Snap the neck off a high range Chlorine Standard Solution Voluette® Ampule,

50–70 mg/L Cl

2

.

b. Use a T enSette

®

pipet to add 0.1, 0.2, and 0.3 mL of standard to three separate

5-mL samples. Swirl gently to mix.

c. Analyze each sample as described in the procedure. Each 0.1 mL of standard

will cause an incremental increase in chlorine. The exact value depends on the
concentration of the Voluette ampule standard. Check the certificate enclosed
with the Voluette ampules for calculation of the expected increase in the
chlorine concentration.

Standard Solution Method

Standard solutions for chlorine are difficult and time-consuming to prepare. Errors can
occur if attention to detail is not addressed during preparation of the standards. The
calibration curve is prepared under rigorous analytical laboratory conditions. Use the
factory calibration fo r most normal testing.

1—45

Chlorine, Free and T otal, HR, continued

A user calibration or a user-prepared chlorine standard may be required by a regulatory
official or agency. Two options are available on the Pocket Colorimeter II to meet this
requirement.

A chlorine standard may be prepared and used to validate the calibration curve using
the Standard Calibration Adjust feature (see page 2—13 for more information). The
concentration of the prepared standard must be determined with an alternate
instrument such as a spectrophotometer, colorimeter, or by using an alternate method
such as amperometric titration. The concentration of the chlorine standard for the HR
procedure must be between 4.5 and 7.0 mg/L chlorine.

In addition, a user-generated calibration curve can be pr ogrammed into the
Pocket Colorimeter II. See User-Entered Calibration on page 2—15 for more
information.

1—46

Chlorine, Free and Total, HR, continued
Interferences

Interfering
Substance

Interference Levels and Treatments

Acidity Greater than 150 mg/L CaCO3. May not develop full color or

color may fade instantly. Neutralize to pH 6–7 with 1 N
Sodium Hydroxide. Determine amount to be added on separate
5-mL sample, then add the same amount to the sample being
tested. Correct for the additional volume.

Alkalinity Greater than 250 mg/L CaCO

3

. May not develop full color or
color may fade instantly. Neutralize to pH 6–7 with 1 N
Sulfuric Acid. Determine amount to be added on separate 5-mL
sample, then add the same amount to the sample being tested.
Correct for the additional volume.

Bromine, Br

2

Interferes at all levels

Hardness No effect at less than 1,000 mg/L as CaCO

3

Iodine, I

2

Interferes at all levels

1—47

Chlorine, Free and T otal, HR, continued

Manganese,
Oxidized
(Mn

4+

, Mn7+) or
Chromium,
Oxidized (Cr

6+

)

1. Adjust sample pH to 6–7.

2. Add 3 drops Potassium Iodide (30-g/L) (Cat. No. 343-32)

to a 5-mL sample.

3. Mix and wait one minute.

4. Add 3 drops Sodium Arsenite (5-g/L) (Cat. No. 1047-32)

and mix.

5. Analyze 5 mL of the treated sample as described in

the procedure.

6. Subtract the result from this test from the original analysis

to obtain the correct chlorine concentration.

Interfering
Substance

Interference Levels and Treatments

1—48

Chlorine, Free and Total, HR, continued

Monochloramine For conventional free chlorine disinfection (beyond the

breakpoint), monochloramine concentrations are very low. If
monochloramine is present in the sample, its interference in the
free chlorine test varies with the temperature, the relative
amount of monochloramine to free chlorine, and the time
required to do the analysis. Approximate interference levels of
monochloramine in the free chlorine test are listed below (as
mg/L Cl

2

).

Ozone Interferes at all levels.

Interfering
Substance

Interference Levels and Treatments

NH2Cl Sample Temperatu re °C (°F)

(as Cl2) 5 (40) 10 (50) 20 (68) 30 (83)

1.2 0.2 0.2 0.3 0.3

2.5 0.4 0.5 0.6 0.6

3.5 0.5 0.6 0.7 0.8

1—49

Chlorine, Free and T otal, HR, continued

Spec√ Secondary Standards

The DPD Chlorine Secondary Standards kit (Cat. No. 26353-00) cannot be used on the
high range (HR) chlorine channel.

Method Performance

Estimated Detection Limit (EDL) = 0.1 mg/L Cl

2

Typical Precision (95% confidence interval) = 5.0 ± 0.2 mg/L Cl

2

Summary of Method

Chlorine can be present in water as free chlorine and as combined chlorine. Both forms
can coexist in the same solution and can be determined together as total chlorine. Free
chlorine is present as hypochlorous acid or hypochlorite ion. Combined chlorine
represents a combination of chlorine-containing compounds including but not limited
to monochloramine, dichloramine, nitrogen trichloride, and other chloro derivatives.
The combined chlorine oxidizes triiodide ion (I

3

–

) to iodine (I2). The iodine and free
chlorine reacts with DPD (N,N-diethyl-p-phenylenediamine) to form a red solution.
The color intensity is proportional to the total chlorine concentration. To determine the
concentration of combined chlorine, run a free chlorine test and a total chlorine test.

1—50

Chlorine, Free and Total, HR, continued

Subtract the results of the free chlorine test from the total chlorine test to obtain the
combined chlorine concentration.

The range of analysis using the DPD method for chlorine is extended by adding more
indicator in proportion to sample volume. Thus, a larger, 25-mL size powder pillow of
DPD Reagent is added to a 5-mL sample.

Replacement Parts

Required Reagents

Description U nit C at. No.

Free Chlorine Tests

DPD Free Chlorine Reagent

Powder Pillows for 25-mL sample...........................................100/pkg..... 14070-99

Total Chlorine Tests

DPD Total Chlorine Reagent

Powder Pillows for 25-mL sample...........................................100/pkg..... 14064-99

1—51

Chlorine, Free and T otal, HR, continued

Optional Reagents

Description Unit Cat. No.

Chlorine Standard Solution, Voluette Ampules,

50–75 mg/L, 2-mL.....................................................................20/pkg..... 14268-20

Potassium Iodide Solution, 30 g/L ...................................100 mL MDB*......... 343-32

Sodium Arsenite Solution, 5 g/L........................ ................100 mL MDB....... 1047-32

Sodium Hydroxide Standard Solution, 1 N........................100 mL MDB....... 1045-32

Sulfuric Acid Stan d a rd Solution, 1 N.................................100 mL MDB....... 1270-32

Water, deionized ..................................................................................4 L......... 272-56

Optional Apparatus

AccuVac® Snapper Kit......................................................................each..... 24052-00

Batteries, AAA, alkaline.......................... ........................................4/pkg..... 46743-00

Caps for 10-mL sample cells.........................................................12/pkg..... 24018-12

Cylinder, graduated, 25 mL. glass.....................................................each......... 508-40

Cylinder, graduated, 100 mL, glass...................................................each......... 508-4 2

sens

ion™1 Basic Portable pH Meter, with electrode......................each..... 51700-10

* Marked Dropper Bottle

1—52

Chlorine, Free and Total, HR, continued

Optional Apparatus, continued

Description U nit C at. No.

Pipet, TenSette®, 0.1 to 1.0 mL......................................................... each..... 19700-01

Pipet Tips, For 19700-01 TenSette

®

Pipet.....................................50/pkg..... 21856-96

Replacement Parts

Cap for 1-cm/10 mL sample cell.......................................................each..... 52626-00

Instrument Cap/light shield................................................................each..... 59548-00

Instruction Manual.............................................................................each..... 59571-88

Sample Cells, 1-cm/10-mL............... .................................. .............2/pkg..... 48643-02

1—53

Chlorine Dioxide (0.05 to 5.00 mg/L ClO

2

)

Method 10126

For water and wastewater

DPD Method* USEPA accepted for drinking water **

Measuring Hints

• Chlorine dioxide samples cannot be stored, they must be analyzed immediately

after collection to minimize loss of chlorine dioxide.

• Do not use plastic sample containers or measurement apparatus.

• If the chlorine dioxide concentration exceeds the upper limit of the test (5.00 mg/L

ClO

2

), the color will not develop properly. The display will show overrange

(flashing 5.50) when the concentration is 5.50 or more.
If the reading is over 5.00, dilute the sample with high quality water that is
chlorine demand-free and repeat the test. Some loss of chlorine dioxide may occur
during dilution. Multiply the result by the appropriate dilution factor.

* Adapted from Standard Methods for Examination of Water and Wastewater.
** Procedure is equivalent to Standard Methods, 4500 ClO

2

D.

1—54

Chlorine Dioxide, continued

• Use a timer for reagent reaction and be consistent for all samples.

• Gently swirl samples to mix reagent s. Do not shake sample cells or ampules. Do
not invert sample cells. AccuVac Ampuls can be inverted as they contain little air.
Vigorous agitation may cause loss of volatile chlorine dioxide before it can react.

• Allow any unreacted reagent to settle before taking readings.

• For best accuracy when using AccuVac Ampuls, draw sample into an empty
AccuVac ampule (Cat. No. 26779-25) and use that for the zero instead of a 10-mL
sample cell.

• Check the reagent blank for each new lot and at least every two months. Make this
adjustment by using deionized water in the procedure instead of sample and
subtract that reading from future test results.

Note: The Pocket Colo rimeter™ II is designed to measure solutions contained in sample cells.

DO NOT dip the meter in the sample or pour the sample directly into the cell holder.

1—55

Chlorine Dioxide, continued

Chlorine Dioxide (using Powder Pillows)

1.Fill a 10-mL sample cell

with sample (the blank).
Note: Samples must be

analyzed immediately.

2.Press the POWER key to

turn the meter on.
The arrow should indicate

channel 1.
Note: See page 2—4 for

information on selecting the
correct range channel.

3.Place the cell into the cell

holder. Cover the cell with
the instrument cap.

1—56

Chlorine Dioxide, continued

4.Press ZERO/SCROLL.

The display will show
“- - - -” then “0.00”.

Remove the blank from the
cell holder.

5.Fill a second sample cell

with 10 mL of sample.
Immediately add four drops
of Glycine Reagent to the
sample cell and swirl to mix.

6.Immediately add the

contents of one DPD Free
Powder Pillow. Cap and
swirl gently for 20 sec. Wait
30 sec. for undissolved
powder to settle. Accuracy is
not affected by undissolved
powder.

Note: A pink color will

develop if ClO

2

is present.

1—57

Chlorine Dioxide, continued

7.Within one minute of

adding the DPD to the
sample, place the prepared
sample into the cell holder.

8.Cover the sample with

the instrument cap.

9.Press READ/ENTER.

The display will show
“- - - -”, followed by results
in mg/L ClO

2

.

1—58

Chlorine Dioxide, continued

Chlorine Dioxide (using AccuVac® Ampuls)

1.Fill a 10-mL sample cell

with sample (the blank).
Note: Samples must be

analyzed immediately.

2.Press the POWER key to

turn the meter on.
The arrow should indicate

channel 2.
Note: See page 2—4 for

information on selecting the
correct range channel.

3.Place the cell into the cell

holder. Cover the cell with
the instrument cap.

1—59

Chlorine Dioxide, continued

4.Press ZERO/SCROLL.

The display will show
“- - - -” then “0.00”.

Remove the blank from the
cell holder.

5.Collect 40 mL of sample

in a 50- or 100-mL beaker.
Add 16 drops of Glycine
Reagent to the 40 mL of
sample. Swirl gently to mi x.
Continue with step 6
immediately.

6.Fill a DPD Free Chlorin e

Reagent AccuVac Ampul
with the pretreated sample in
the beaker.

Note: Keep the tip immersed

until the ampule fills
completely.

1—60

Chlorine Dioxide, continued

7.Quickly invert the

ampule several times to mi x.
Wait 30 seconds for any
undissolved powder to
settle. Accuracy is not
affected by undissolved
powder.

Note: A pink color will form

if chlorine dioxide is
present.

8.Within one minute after

filling the ampule, place it
into the cell holder. Cover
the ampule with the
instrument cap.

9.Press READ/ENTER.

The display will show
“- - - -” followed by the
results in mg/L chlorine
dioxide.

1—61

Chlorine Dioxide, continued

Sampling and Storage

Analyze samples for chlorine dioxide immediately after collection. Chlorine dioxide is
a strong oxidizing agent and is unstable in natural waters. It reacts rapidly with various
inorganic compounds, but oxidizes organic compounds more slowly. Many factors,
including reactant concentrations, sunlight, pH, temperature, and salinity influence
decomposition of chlorine dioxide in water.

Do not use plastic containers to collect samples for analysis since they often have a
high chlorine dioxide demand. Pretreat glass sample containers to remove any chlorine
or chlorine dioxide demand by soaking in a dilute bleach solution (1 mL commercial
bleach to l liter of deionized water) for at least one hour. Rinse thoroughly with
deionized or distilled water. If sample containers are rinsed thoroughly with deionized
or distilled water after each use, only occasional pretreatment is necessary thereafter.

Failure to obtain a representative sample is a common error in testing for chlorine
dioxide. If sampling from a tap, let the water flow for at least 5 minutes to ensure a
representative sample. Let the container overflow with the sample several times, then
cap the sample containers so there is no headspace (air) above the sample. If sampling
with a sample cell, rinse the cell several times with the sample, then carefully fill to the
10-mL mark. Perform the chlorine dioxide analysis immediately.

Accuracy Check

1—62

Chlorine Dioxide, continued

Because chlorine dioxide is difficult and hazardous to produce, check the DPD and
Glycine Reagents by using chlorine standards. Proceed as follows:

1. Prepare a 1-mg/L free chlorine standard.
a. Obtain Free Chlorine Standards (Cat. No. 14268-10).
b. Determine the concentration of the standard from the certificate of analysis

shipped with the standard (50–75 mg/L Cl

2

). Calculate the volume of standard

needed as follows:
mL standard needed = 100

÷ standard concentration

c. Pipet the volume of standard needed into a 100-mL volumetric flask. Dilute to

the line with chlorine demand-free deionized water. Invert to mix.

2. Perform the chlorine dioxide test on the standard without adding Glycine Reagent
(step 3 of the procedures).

3. The chlorine dioxide reading should be 2.4 times greater than the chlorine
concentration. If so, this verifies that the DPD Reagent and the instrument are
working properly.

1—63

Chlorine Dioxide, continued

4. Repeat the chlorine dioxide test on the chlorine standard, including the Glycine

addition. The reading should be less than 0.10 mg/L ClO

2

. This verifies that the

Glycine is eliminating free chlorine interference.

Standard Solution Method

Standard solutions for chlorine dioxide are difficult and time-consuming to prepare.
Errors can occur if attention to detail is not addressed during preparation of the
standards. Hach prepares the calibration curve under rigorous analytical laboratory
conditions. Hach recommends using the factory calibration.

A user calibration or a user-prepared chlorine dioxide standard may be required by a
regulatory official or agency . T wo options are available on the Pocket Colorimeter II to
meet this requirement.

A chlorine dioxide standard may be prepared and used to validate the calibration curve
using the Standard Calibration Adjust feature (see Standard Calibration Adjust on

page 2—13). The concentration of the prepared standard must be determined with an

alternate instrument such as a spectrophotometer, colorimeter, amperometric titration
or by using another alternate method. The concentration of the chlorine dioxide
standard must be between 2.00 and 4. 50 mg /L ch lorine dioxide.

1—64

Chlorine Dioxide, continued

In addition, a user-generated calibration curve can be made and programmed into the
Pocket Colorimeter™ II. See User-Entered Calibration on page 2—15.

Method Performance

Typical Precision (95% confidence interval):

4.78 ± 0.22 mg/L ClO

2

(AccuVac)

2.08 ± 0.04 mg/L ClO

2

(Powder Pillows)

Estimated Detection Limit:

EDL = 0.04 mg/L ClO

2

(AccuVac and Powder Pillows)

Interferences

Samples containing more than 250 mg/L alkalinity or 150 mg/L acidity as CaCO3 may
inhibit full color development, or the color may fade instan tly. Neutralize these
samples to pH 6–7 with 1 N Sulfuric Acid Standard Solution or 1 N Sodium Hydroxide
Standard Solution. Determine the amount required on a separate 10-mL sample. Add
the same amount to the sample to be tested. Correct for the additional volume.

1—65

Chlorine Dioxide, continued

Samples containing monochloramine will cause a gradual drift to higher chlorine
dioxide readings. When read within one minute of reagent addition, 3.0 mg/L
monochloramine will cause an increase of less than 0.10 mg/L in the chlorine dioxide
reading.

Bromine, chlorine, iodine, ozone and oxidized forms of manganese and chromium also
interfere and read as chlorine dioxide.

T o compensate for the effects of manganese (Mn

4+

) or chromium (Cr6+), adjust the pH
to 6–7 as described above. T o a 25-mL sample, add 3 drops of 30 g/L Potassium Iodide
Solution, mix, and wait one minute. Add 3 drops of 5 g/L Sodium Arsenite Solution
and mix. Subtract the result of this test from the original analysis to obtain the accurate
chlorine dioxide concentration.

DPD Free Chlorine Reagent Powder Pillows and AccuVac Ampuls contain a buffer
formulation that withstands high levels (at least 1000 m g/L) of hardness without
interference.

Organic chloramines and peroxides may interfere. Various metals may interfere by
combining with the Glycine Reagent needed to remove the chlorine interference.
Metal interference is limited except when chlorine is present. In the presence of 0.60
mg/L Cl

2

, both copper (> 10 mg/L) and nickel (> 50 mg/L) interfere. Other metals may

1—66

Chlorine Dioxide, continued

also interfere depending on their ability to prevent Glycine from reacting with any
chlorine in the sample. It may be necessary to add more Glycine to overcome this
interference.

Summary of Method

Twenty percent of the chlorine in the chlorine dioxide is reduced to chlorite, which
reacts with DPD (N,N-diethyl-p-phenylenediamine) indicator to form a pink color.
The color intensity is proportional to the ClO

2

in the sample. Chlorine interference is
eliminated by adding Glycine Reagent, which converts free chlorine to
chloroaminoascorbic acid, but has no effect on chlorine dioxide at the test pH.

Reagents and Apparatus

Required Reagents (Using Powder Pillows)

Description Units Cat. No.

ClO2 DPD Powder Pillow/Glycine Reagent Set (100 tests)..................... ..... 27709-00

Includes:
(1) DPD Free Chlorine Reagent Powder Pillows, 100/pkg
(1) Glycine Reagent, 29 mL

1—67

Chlorine Dioxide, continued

Required Reagents (Using AccuVac Ampuls)

Description Units Cat. No.

ClO2 DPD AccuVac®/Glycine Reagent Set (25 tests)............................ . ..... 27710-00

Includes:
(1) DPD Free Chlorine Reagent AccuVac

®

Ampuls, 25/pkg

(1) Glycine Reagent, 29 mL

Require Apparatus (Using AccuVac Ampuls)

Beaker, 50-mL, glass.........................................................................each......... 500-41

Beaker, 100-mL, glass.......................................................................each......... 500-42

Optional Reagents

AccuVac® Ampuls for Blanks.......................................................25/pkg..... 2 6779-25

Chlorine Standard Solution Voluette™ Ampules,

50–75 mg/L, 10-mL...................................................................16/pkg..... 14268-10

Potassium Iodide Solution, 30 g/L ....................................100-mL MDB......... 343-32

Sodium Arsenite Solution, 5 g/L........................ ...............100-mL MDB....... 1047-32

1—68

Chlorine Dioxide, continued

Optional Reagents, continued

Description Units Cat. No.

Sodium Hydroxide Standard Solution, 1 N.......................100-mL MDB....... 1045-32

Sulfuric Acid S tan dard Solution, 1 N........................... .....100-mL MDB....... 1270-32

Water, deionized ..................................................................................4 L.........272-56

Optional Apparatus

AccuVac® Snapper Kit......................................................................each..... 24052-00

Caps for 10-mL sample cells.........................................................12/pkg..... 24018-12

Cylinder, graduated, 25-mL, glass.....................................................each......... 508-40

Cylinder, graduated, 100-mL, glass...................................................each......... 508-42

sension™1 Basic Portable pH Meter, with electrode........................each..... 51700-10

Pipet, TenSette

®

, 0.1 to 1.0 mL......................................................... each..... 19700-01

Pipet Tips, for 19700-01 TenSette

®

Pipet......................................50/pkg..... 21856-96

Replacement Parts

Batteries, AAA, alkaline............................ ......................................4/pkg..... 46743-00

Instrument Cap/light shield................................................................each..... 59548-00

Instrument Manual.............................................................................each..... 59571-88

Sample Cell, 25 x 60 mm 10 mL mark............................................6/pkg..... 24276-06

1—69

pH (6.0 to 8.5 pH units)

For water and wastewater

Method 1 0 076

Phenol Red Method

Measuring Hints

• For greatest accuracy, use the Standard Calibration Adjust to adjust the

calibration curve with each new lot of Phenol Red. Use the pH 7.00 Buffer
Solution included in the kit.

• Periodically, recheck with the pH 7 Bu ffer to confirm the accuracy of the

instrument and method. If not rea ding within 0.1 pH unit, recalibrate th e
instrument.

• The amount of indicator a nd sample is critical for accurate results and sh ould

be measured carefully.

• Attempting to measure a sample with a pH below 6.0 or above 8.2 will result

in an inaccurate reading. Measurements outside the range can differ from the
true pH reading by as much as 2 pH units.

Note:

The Pocket Colorimeter II is designed to measure solutions contained in sample cells.

DO NOT

dip the meter in the sample or pour the sample directly into the cell holder.

1—70

pH, continued

1.Collect about 50 mL of

sample in a beaker and fill a
10-mL sample cell to the
10-mL mar k with sample.

2.Pr ess the POWER key to

turn the meter on.
The arrow sh ould indicate

pH.

Note:

See page 2—4 for
information on selecting the
correct range channel.

3.Using the calibrated

dropper, add 0.5 mL of
Phenol Red Solution to the
cell. Cap and invert to mix.

Note:

For greatest accuracy,

use a clean and dry dropper.

1—71

pH, continued

4.Fill a second sample cell

to the 1 0-mL mark with the
original sample. Cap the
sample cell. This is the
blank.

5.Place the blank into the

cell holder.

Note:

Wipe liquid off sample
cells or damage to the
instrument may occur.

6.Cover the cell with the

instrument cap.

1—72

pH, continued

7.Press ZERO/SCROLL.

The display will show
“- - - -” then “0.0”.

Remove the blank fro m the
cell holder.

8.Place the prepared

sample in the cell holder.

Note:

Wipe liquid off sample

cells.

9.Cover the cell with the

instrument cap.

1—73

pH, continued

10. Press READ/ENTER.

The display will show
“- - - -”, followed by results
in pH units.

1—74

pH, continued
Accuracy Check

Preparation of pH 7.0 Standard Solution

A pH 7.00 standard solution is necessary to calibrate or check the accuracy of the pH
measurement using the Standard Calibration Adjust feature (see Standard Calibration

Adjust on page 2—13). Use the pH 7.00 Buffer Vial (in cluded in this kit) or bulk buffer

solution (see Optional Reagents on page 1—76). Add 0.5 mL of Phenol Red Solution to
10 mL of pH 7.00 Buffer Solution to prepare the color standard. Do not use col ored
buffer solutions.

For greatest accuracy, adjust the calibration curve using Standard Calibration Adjust
with each new lot of Phenol Red Indicator. Periodically check the strength of the
indicator by testing a pH 7.0 Buffer Solution.

Method Performance

Typical Precision (95% CI):

7.0 ± 0.1 pH

1—75

pH, continued

Standard Calibration Adjust

To perform a standard calibration adjustment using the pH 7.0 buffer, see Standard

Calibration Adjust on page 2—13.

Summary of Method

Proper pH control in drinking water and swimmin g po ols is necessary fo r m an y
reasons. Bactericidal activity of chlorine is greatest when the pH is less than 7.4.
Alum-type flocculents, important for clear water, perform best in a pH range of 7.2
to 8.0. Keeping the pH below 8.0 is important to control algae. A sudden rise in pH
may signal an increase in algal growth. If the pH drops below 7.0, corrosion of
pipes and metal fixtures may occur. Eye irritation is minimized when the pH is kept
between 7.2 and 7.6.

Phenol Red is a phthalein indicator which has a transition window of pH 6.8
(yellow) to 8.2 (red). The pH is determined colorimetrically from the change in the
absorbance of the alkaline peak.

1—76

pH, continued
Replacement Parts

Required Reagents

Description Unit Cat. No.

Buffer Solution, pH 7.0....................................................................25 mL ....12222-20

Phenol Red Solution, Spec-grade...................................................50 mL ....26575-12

Required Apparatus

Sample Cells, 10 mL, with caps.......................................................6/pkg ....2427 6-0 6

Optional Reagents

Buffer Solution, pH 7.0................................................................. 500 mL ....12222-49

Water, deionized................................................... ......................... 500 mL . ....... 272-49

Optional Apparatus

Droppers, 0.5 and 1.0 mL.............................................................. pkg/20 .... 21247-20

Replacement Parts

Batteries, AAA, alkaline ...................................................................4/pkg ....46743-00

Instrument Cap/light shield................... .............................................each ....59548-00

Instruction Manual.......................... ....... .............................................each ..... 5 95 71-88

2—1

Section 2

Instrument Manual

2—2

2—3

Instrument Operation

Key Functions

Key Description Function

POWER On/Off/Backlight

To turn on the backlight, turn on the instrument, then
press and hold the power key until the backlight turns
on. Press and hold again to turn off the backlight. This
key functions the same in all instrument modes and
ranges.

ZERO/SCROLL In measurement mode, sets the instrument to zero.

In menu mode, scrolls through menu options. Also
scrolls numbers when entering or editing a value.

READ/ENTER In measurement mode, initiates sample measurement.

In menu mode, selects a menu option. When entering
numbers, moves one space to the right and executes
the function when the entry is complete.

2—4

Instrument Operation, continued

Menu Selections

Press the MENU key to access the menu selections.

Switching Ranges

1. Press the MENU key. The display will show “SEL”. A flashing arrow indicates
the current range.

2. Press the

READ/ENTER key to toggle between ranges.

3. Press

MENU again to accept and exit back to the measurement screen.

Setting the Time

1. Press the MENU key, then press the ZERO/SCROLL key un til th e di sp lay shows
a time in the “00:00” format.

MENU Enter/Exit the menu mode

Press and hold for approximately 5 seconds to enter
user-entered method mode.

Key Description Function

2—5

Instrument Operation, continued

2. Press READ/ENTER. The digit to be edited will flash.

3. Use the

ZERO/SCROLL key to change the entry, then press READ/ENTER to

accept and advance to the next digit. The time is entered in 24-hour format.

Recalling Stored Measurements

1. Press the MENU key, then press the ZERO/SCROLL key until the display shows

RCL. The instrument automatically stores the last 10 measurements.

2. In RCL, press

READ/ENTER to recall the stored measurements, beginning with

the most recent measurement taken. The meter stores the measurement
number as 0 1 (most recent) through 1 0 (oldest), the time the measurement was
taken, and the measurement value. The

ZERO/SCROLL key allows for selection

of a specific measurement by number. The

READ/ENTER key scrolls through all

stored data points.

2—6

Instrument Operation, continued

Battery Installation

Figure 1 on page 2—7 provides an exp lod ed vi ew of battery installation.

1. Unhook the latch and remove the bat tery co mpartment cover. The polarities
are shown on the battery holder.

2. Place the four batteries provided with the instrument in the holder as indicated
and replace the battery compartment cover. The display will show the software
version number (e.g., “P 1.6”) after correct battery installation.

When replacing discharged batteries, always replace the complete set of four
alkaline batteries. Rechargeable batteries are not recommended and cannot be
recharged in the instrument.

Note:

The Low Battery icon will appear on the display when the batteries have 10 % battery life
remaining. The battery icon will flash when the batteries are too low to complete
measurements. See Instrument Keys and Display on page 1—11.

2—7

Instrument Operation, continued

Figure 1 Battery Installation

2—8

2—9

Error Codes

When the instrument canno t perfo r m th e funct io n in it iat ed by the operator, an
error message will appear in the display. Refer to the appropriate message
information below to determine what the problem is and how it can be corrected.
Resolve error messages in the order that they appear on the display. Service Centers
are listed in page 2—37.

Error Messages

1. E-0 No Zero (User mode)

Error occurs when trying to read a standard in the user calibration mode
before setting the meter to zero.

• Zero the instrument on an appropriate blank.

2. E-1 Ambient Light Er ro r

There is too much light present to take a valid measurement.

• Verify instrument cap is correctly seated.

• If the problem persists, contact a Service Center (page 2—37).

2—10

Error Codes, continued

3. E-2 LED Error

The LED (light source) is out of regulation.

• Replace batteries.

• Verify LED lights up (inside the cell holder) when the READ/ENTER or

ZERO/SCROLL key is pressed.

• If the problem persists, contact a Service Center (page 2—37).

Note: When an E-1 or E-2 error occurs on a measurement, the display will show “_.__”. (The

decimal place is determined by the chemistry.) If the E-1 or E-2 error occurs while
zeroing the meter , the meter will require the user to re-zero.

4. E-3 Standard Adjust Error

The value obtained on the prepared standard exceeds the adjustment limits
allowed for the standard concentration, or the concentration of the standard is
outside the concentration range allowed for standard calibration adjust.

• Prepare the standard and rerun according to the procedure.

• Prepare a standard at or near the recommended concentrations given in the

procedure.

• Verify that the concentration of the standard has been entered correctly.

2—11

Error Codes, continued

• If the problem persists, contact a Service Center (page 2—37).

5. E-6 Abs Error (User mode )

Indicates that the absorbance value is invalid, or indicates an attempt to make
a curve with less than two points.

• Enter or measure the absorbance value again.

• If the problem persists, contact a Service Center (page 2—37).

6. E-7 Standard Value Error (User mode)

Standard concentratio n is equ al to another standard concentr ati on tha t is
already entered.

• Enter the correct standard concentration.

• If the problem persists, contact a Service Center (page 2—37).

7. E-9 Flash Error

The meter is unable to save data.

• If the problem persists, contact a Service Center (page 2—37).

2—12

Error Codes, continued

8. Underrange—flashing number below stated test range

• Verify instrument cap is correctly seated.

• Check zero by measuring a blank. If error recurs, re-zero the instrument.

• If the problem persists, contact a Service Center (page 2—37).

Note: See Maximum/Minimum Displayed Value on page 2—26 for more information.

9. Overrange—flashing number above stated test range

Note: Flashing value will be 10% over the upper test limit.

• Check for light blockage.

• Dilute and retest sample.

Note: See Maximum/Minimum Displayed Value on page 2—26 for more information.

2—13

Standard Calibration Adjust

The Pocket Colorimeter™ II instrument is factory-calibrated and ready for use
without user calibration. Use of the factory calibration is recommended unless the
user is required to generate a calibration. The Standard Calibration Adjust can be
used to meet regulatory requirements.

This feature allows the factory default calibration curve to be adjusted with a
known standard. Use the standard described in the procedure.

1. Place a blank in the meter (in measurem ent mo de). Press

ZERO/SCROLL.

2. Place the reacted standard in the meter. Press

READ/ENTER.

3. Press

MENU, then press ZERO/SCROLL until the display shows “SCA”.

4. Press

READ/ENTER to display the standa rd calibration adjust value.

5. Press

READ/ENTER to adjust the curve to the displayed value. The meter will

return to the measurement mode and the Calibration Adjusted icon will appear
in the display window.

If an alternate concentration is used, or if a standard concentration is not given:

6. Repeat steps 1–4.

2—14

Standard Calibration Adjust, continued

7. Press ZERO/SCROLL to access the Edit function, then press READ/ENTER to
begin editing. The digit to be edited will flash. Use the

ZERO/SCROLL key to

change the entry, then press

READ/ENTER to accept and advance to the

next digit.
When the last digit is entered, press

READ/ENTER and the meter will adjust the

curve to the value entered. The meter will return to measurement mode and the
Calibration Adjusted icon will appear in the display window.

To turn off Standard Calibration Adjust (SCA):

1. Press

MENU.

2. Press

ZERO/SCROLL until “SCA” appears in the display.

3. Press

READ/ENTER, then press ZERO/SCROLL until “Off” appears in the display.

4. Press

READ/ENTER to turn off SCA.

Note: Perform another standard calibration adjust to turn SCA on again.
Note: For meters with factory-calibrated ranges or methods, Standard Calibration Adjust

(SCA) will be disabled when a user-entered method is programmed into the meter. To
turn SCA back on, restore the meter to factory default calibration. See Retrieving the
Factory Calibration on page 2—25.

2—15

User-Entered Calibration

Overview

The Pock et Colorimeter™ II will accept a user-prepared calibration curve. The curve
can extend from 0 to 2.5 absorbance. A user-prepared calibration curve may be
entered into any channel that does not contain a factory-progr ammed curve. These
channels are labeled “abs” on instruments having a single factory calibration or
are labeled “1” and “2” on the uncalibrated single wavelength instruments. Any
chemistry that can be run at the instrument wavelength may be user-entered in
these channels.

Using prepared standard solutions that cover the range of interest, th e mete r
generates a calibration curve by calculatin g th e stra igh t-l in e segments between
each standard entered. A calibration curve may be entered using the keypad.
Factory-entered calibration curves may also be recalculated or adjusted using the
same procedure.

To enter the user-entered calibration mode, press the

MENU key and hold it down

until the display shows “USER” (about 5 seconds), followed by “CAL”. Press

ZERO/SCROLL to scroll through the options.

2—16

User-Entered Calibration, continued

• CAL—Used to enter and edit standard values and measure absorbance values,

or review the existing calibration.

• Edit—Used to enter and edit standard values and ab sorbance values with the

keypad or review the existing calibration. Used to enter a predetermined
calibration curve.

• dFL—Used to return the instrument back to the default factory calibration.

User-entered calibrations are stored upon exit from the calibration or
edit modes.

Note: To return to factory settings, following the instructions in Retrieving the Factory

Calibration on page 2—25.

If the instrument is shut off or loses power during data entry, all edits will be lost.
Automatic shut-off in user-entered cali br at io n entry mode is 60 minutes.

CAL and Edit Submenus

In CAL mode, standard values are entered and absorbance values are measured. In
Edit mode, standard and absorbance values are entered.

• To select CAL from the User menu, press READ/ENTER.

• To sel ect Edit from the User menu, press ZERO/SCROLL and READ/ENTER.

2—17

User-Entered Calibration, continued

• Once in the CAL or Edit optio n, press the READ/ENTER key to navigate through

each option.

Note: Press

ZERO/SCROLL

to quickly scroll through each option.

Calibration Procedure Using Prepared Standards

Note:

Deionized water or a reagent blank can be used to zero during the calibration procedure.
Calibrations generated with deionized water as the zero will give less accurate results if
the reagent blank is significantly more turbid or colored than deionized water. Use the
deionized water or the reagent blank as the zero concentration point (S0) in the following
calibration procedure.

1. Turn on the instrument and select the range to be calibrated. An arrow at the
top of the display will point to the selected range. To change ranges, press the

MENU key, then use the READ/ENTER key to toggle between ranges 1 and 2.

Press

MENU again to return to measurement mode.

2. Follow the procedure for the chemical method to be calibrated . Prepare a
reagent blank (if needed) and a standard solution. Allow the color to
develop fully.

2—18

User-Entered Calibration, continued

3. Insert the reagent blank or deionized water into the meter and cover with the
cap. Press the

ZERO/SCROLL key . Th e meter will displ ay “- - - -”, followed by

“0.000”. This initializes (zeroes) the meter.

4. Press the

MENU key and hold it down until the display shows “USER”, followed

by “CAL”. Press

READ/ENTER to enter the calibration mode.

5. In factory-calibrated meters, S0 will appear in the display.

Note: When recalibrating a factory-calibrated meter or range, RES (resolution) cannot

be changed.

6. In uncalibrated meters or meters with ranges labeled Abs, “RES” will appear.
Press

ZERO/SCROLL to review the current resolution (decimal placement). Press

ZERO/SCROLL again to accept the current resolution. To change the resolution,

press

READ/ENTER, then ZERO/SCROLL to change the resolution. Press

READ/ENTER to accept the new resolution. “S0” will appear on the display.

7. Press the

READ/ENTER key again, then enter the blank value.

Note: Press the

READ/ENTER

key to move from digit to digit. Use the

ZERO/SCROLL

key to

change the number.

8. After completing entry of the blank value, press the READ/ENTER key. The
display will show “A0”.

2—19

User-Entered Calibration, continued

9. Insert the reagent blank or deionized water into the cell holder. Cover the

blank with the instrument cap.

10. Press the

READ/ENTER key . The meter will measure and display the absorbance

value for “S0”.

11. Remove the sample blank. Press the

ZERO/SCROLL key . “S1” will appear . Press

the

READ/ENTER key, then enter the first standard value.

Note: Press the

READ/ENTER

key to move from digit to digit. Use the

ZERO/SCROLL

key to

change the number.

12. After completing entry of the first standard value, press the READ/ENTER key.

The display will show “A1”.

13. Insert the first reacted standard solution into the cell holder. Cover the

prepared standard with the instrument cap.

14. Press the

READ/ENTER key . The meter will measure and display the absorbance

value for S1.

15. The calibration is complete with two points. If additional standards are

required, press

ZERO/SCROLL until “Add” appears on the display. Repeat

steps 11–14 to enter additional standards.

2—20

User-Entered Calibration, continued

16. Press the MENU key twice to exit and accept the changes. The instrument will
use this calibration to determine the displayed concentration of future sample
measurements.

Entering a Predetermined Calibration Curve

Note: Entering a predetermined calibration curve requires at least two data pairs. Each data

pair requires a concentration value and the absorbance value for the given
concentration. Up to 10 data pairs may be entered. This procedure uses the
Edit mode.

1. Turn on the instrument and select the range to be calibrated. An arrow at the
top of the display will point to the selected range. To change ranges, press the

MENU key, then use the READ/ENTER key to toggle between ranges 1 and 2.

Press

MENU again to return to measurement mode.

2. Press the

MENU key and hold it down until the display shows “USER”, followed

by “CAL”. Press

ZERO/SCROLL to scroll to EDIT. Press READ/ENTER.

3. In uncalibrated meters or in Abs range, “RES” will appear. Press ZERO/SCROLL.
To change the resolution (decimal placement), press

READ/ENTER. Press

ZERO/SCROLL to select the new resolution, then press READ/ENTER to accept.

“S0” will appear on the display.

2—21

User-Entered Calibration, continued

4. Enter the concentration value and absorbance value of the first data pair

(S0, A0).

5. To enter the S0 value, press

READ/ENTER. Use the ZERO/SCROLL key to select

the numerical value, then press the

READ/ENTER key to accept the entry and

advance to the next decimal place. Repeat this sequence until the S0
concentration value is entered.

6. After editing the S0 value, press

READ/ENTER to accept. “A0” will appear on

the display.

7. To enter the absorbance value for S0, press the

READ/ENTER key to go to entry

mode. Use the

ZERO/SCROLL key to select the numerical value, then press the

READ/ENTER key to accept the entry and advance to the next decimal place.

Repeat this sequence until the absorbance value for S0 is entered.

8. After enterin g A0, press

READ/ENTER to accept. “S1” will appear on the

display.

9. Repeat steps 5 through 8 for each standard value and absorbance value pair in

the calibration curve

Note: After A1 is entered, Add will appear in the display. If additional data pairs are to be

entered, press

READ/ENTER

and continue with step 9.

2—22

User-Entered Calibration, continued

10. When all the calibration data has been entered, press MENU twice to return to
the measurement mode.

Editing a User-entered or Factory Calibration Curve

1. Press the MENU key and hold it down until the display shows “USER”, followed
by “CAL”. Press

ZERO/SCROLL until EDIT appears.

2. Press the

READ/ENTER key to enter Edit mode. In factory-calibrated meters,

“S0” will appear in the display.

Note: When editing a factory-calibrated meter or range, RES (resolution) cannot be changed.
Note: When RES or S0 appears in the display, press

ZERO/SCROLL

to quickly scroll to the

data to be edited.

3. In uncalibrated meters or in Abs range, “RES” will appear . Press ZERO/SCROLL
to review the current resolution. Press

ZERO/SCROLL again to accept the

displayed resolution. To change the resolution (decimal placement), press

READ/ENTER. Press ZERO/SCROLL to select the new resolution, then press
READ/ENTER to accept. “S0” will appear on the di splay.

4. Press

READ/ENTER. The current concentration value for S0 will appear on the

display.

2—23

User-Entered Calibration, continued

5. To edit the S0 value, press READ/ENTER. Use the ZERO/SCROLL key to select the

numerical value, then press the

READ/ENTER key to accept the entry and

advance to the next decimal place. Repeat this sequence until the S0
concentration value is entered.

6. After editing the S0 value, press

READ/ENTER to accept. “A0” will appear on

the display.

7. To edit the absorbance value for S0, press th e

READ/ENTER key to go to entry

mode. Use the

ZERO/SCROLL key to select the numerical value, then press the

READ/ENTER key to accept the entry and advance to the next decimal place.

Repeat this sequence until the absorbance value for S0 is entered.

8. After editing A0, press

READ/ENTER to accept. “S1” will appear on the display .

9. Repeat steps 4 through 8 for each standard value and absorbance value pair in

the calibration curve.

10. When all calibration data has been reviewed or edited, “ADD” will appear in

the display.

11. Press

READ/ENTER to add more calibration points, or press MENU twice to

return to the measurement mode.

2—24

User-Entered Calibration, continued

Note: When a factory calibration curve has been edited, the “calibration adjust” icon will

appear in the display.

Exiting the Calibration Routine

Exit the calibration routine by pressing th e MENU key to return to measurement
mode. The instrument uses the last completed user-entered calibration or the
factory calibration if no user-entered calibration has been completed.

Deleting Calibration Points

1. Select the range containing user-entered calibration points. See Switching

Ranges on page 2—4.

2. Press and hold the

MENU key until “USER”, then “CAL” appears. Press

READ/ENTER.

Note: Calibration points can also be deleted in Edit mode.

3. Press ZERO/SCROLL to select the point to delete (e.g., S0 or S1 or S2). Press

READ/ENTER.

4. The left digit will flash. Press

ZERO/SCROLL until “dEL” appears. (“dEL” will

appear after the numeral 9.)