59577-88
POCKET COLORIMETER™ II
ANALYSIS SYSTEMS
INSTRUCTION MANUAL
Iron
Molybdenum
Ozone
Zinc
© Hach Company, 2006. All rights reserved. Printed in the U.S.A. te/dk 12/06 3ed
1—2
Important Note
This manual is intended for use with the following Pocket Colorimeter II
instruments:
The Pocket Colorimeter II instruments listed above are not interchangeable.
Iron Cat. No. 59530-16
Molybdenum Cat. No. 59530-10
Ozone Cat. No. 59530-04
Zinc Cat. No. 59530-09
1—3
Table of Contents
Safety Precautions............................................................................................... 1—7
Laboratory Safety............................................................................................... 1—7
Use of Hazard Information................................................................................1—7
Precautionary Labels........ ....................................................... ....... ....................1—8
Specifications......................................................................................................... 1—9
Instrument Keys and Display..........................................................................1—13
Instrument Cap Cord.........................................................................................1—14
Iron, Total...............................................................................................................1—17
Measuring Hints ...............................................................................................1—17
Sampling and Storage......................................................................................1—26
Accuracy Check................ ....... ....................................................... ....... ...... .....1 —26
Standard Calibration Adjust Method...................... .......................................1—27
Method Performance........................................................................................1—27
Interferences......................................................................................................1—28
Summary of Method........................................................................................1—29
Reagents and Apparatus.................................................. ................................1—30
1—4
Table of Contents, continued
Molybdenum, Molybdate, LR.........................................................................1—33
Measuring Hints................................................................................................1—33
Sampling and Storage......................................................................................1—38
Accuracy Check...................................... ....... ....... .............................................1—38
Interferences......................................................................................................1—38
Summary of Method.........................................................................................1—38
Replacement Parts.............................................................................................1—38
Molybdenum, Molybdate, HR.........................................................................1—39
Measuring Hints................................................................................................1—39
Sampling and Storage......................................................................................1—44
Accuracy Check...................................... ....... ....... .............................................1—44
Method Performance........................................................................................1—45
Standard Calibration Adjust Method..............................................................1—45
Interferences......................................................................................................1—46
Summary of Method.........................................................................................1—49
Ozone.......................................................................................................................1—53
Measuring Hints................................................................................................1—53
Sampling and Storage......................................................................................1—58
Stability of Indigo Reagent....................................... ....... ................................1—58
1—5
Table of Contents, continued
Accuracy Check................ ....... ....................................................... ....... ...... .....1 —58
Spec
√™ Secondary Standards for Instrument Verification.............. ...... .....1 —5 9
Summary of Method........................................................................................1—61
Reagents and Apparatus.................................................. ................................1—62
Zinc...........................................................................................................................1—63
Measuring Hints ...............................................................................................1—63
Sampling and Storage......................................................................................1—68
Accuracy Check................ ....... ....................................................... ....... ...... .....1 —68
Method Performance........................................................................................1—69
Standard Calibration Adjust Method...................... .......................................1—69
Interferences......................................................................................................1—70
Waste Management..........................................................................................1—70
Summary of Method........................................................................................1—71
Reagents and Apparatus.................................................. ................................1—71
Instrument Operation.........................................................................................2—3
Key Functions..................................................... ....... ......................................... 2—3
Menu Selections.............................. ....................................................... ...... ....... 2—4
Switching Ranges...............................................................................................2—4
Setting the Time ................................................................................................. 2—4
1—6
Table of Contents, continued
Recalling Stored Measurements........................................................................2—5
Battery Installation.............................................................................................2—6
Error Codes..............................................................................................................2—9
Error Messages ....................................................................................................2—9
Standard Calibration Adjust...........................................................................2—13
User-Entered Calibration.................................................................................2—15
Overview............................................................................................................2—15
Calibration Procedure Using Prepared Standards.........................................2—17
Entering a Predetermined Calibration Curve.......... .......................................2—20
Editing a User-entered or Factory Calibration Curve...................................2—22
Exiting the Calibration Routine......................... .............................................2—24
Deleting Calibration Points.......................... ....... ...... .......................................2—24
Retrieving the Factory Calibration ................................................................. 2 —25
Maximum/Minimum Displayed Value....................................................... ....2—26
Certification..........................................................................................................2—29
How to Order........................................................................................................2—35
Repair Service.......................................................................................................2—37
Warranty.................................................................................................................2—38
1—7
Safety Precautions
Please read this entire manual before unpacking, setting up, or operating this
instrument. Pay particular attention to all danger and caution statements. Failure
to do so could result in serious injury to the operator or damage to the equipment.
To ensure the protection provided by this equipment is not impaired, do not use or
install this equipment in any manner other than that which is specified in
this manual.
Laboratory Safety
As part of good laboratory practice, pl eas e familiarize yourself with the reagents
used in these procedures. Read all product labels and the m aterial safety data
sheets (MSDS) before using them. It is always good practice to wear safety glasses
when handling chemicals. Foll ow instructions carefully. Rinse thoroughly if
contact occurs. If you have questions about reagents or procedures, please contact
the manufacturer or distributor.
Use of Hazard Information
If multiple hazards exist, this manu al wi ll use th e sign al wo rd (Danger, Caution,
Note) corresponding to the greatest hazard.
1—8
Safety Precautions, continued
DANGER
Indicates a potentially or imminently hazardous situation which, if not
avoided, could result in death or serious injury.
CAUTION
Indicates a potentially hazardous situation that may result in minor or
moderate injury.
NOTE
Information that requires special emphasis.
Precautionary Labels
Please pay particular attention to labels and tags attached to the instrument.
Personal in ju ry or damage to the instrument could occur if not observed .
This symbol, if noted on the instrume n t, referen ces the instruct io n manual
for operational and/or safety inform ation.
1—9
Specifications
Lamp: Light emitting diode (LED)
Detector: Silicon photodiode
Photometric precision: ± 0.0015 Abs
Filter bandwidth: 15 nm
Wavelength: 600 nm
Absorbance range: 0–2.5 Abs
Dimensions: 3.2 x 6.1 x 15.2 cm (1.25 x 2.4 x 6 inches)
Weight: 0.2 kg (0.43 lb)
Sample cells: 25 mm (10 mL), AccuVac
®
Ampuls
Operating conditions: 0 to 50 °C (32 to 122 °F); 0 to 90% relative humidity
(noncondensing)
Power supply: Four AAA alkaline batteries; approximate life is 2000 tests*
* Backlight usage will decrease battery life.
1—10
1—11
OPERATION
DANGER
Handling chemical samples, standards, and reagents can be dangerous. Review the necessary
Material Safety Data Sheets and become familiar with all safety procedures before handling
any chemicals.
DANGER
La manipulation des échantillons chimiques, étalons et réactifs peut être danger euse. Lir e les Fiches
de Données de Sécurité des Produits (FDSP) et se familiariser avec toutes les procédures de sécurité
avant de manipuler tous les produits chimiques.
PELIGRO
La manipulación de muestras químicas, estándares y reactivos puede ser peligro s a. Revi se las fichas
de seguridad de materiales y familiarícese con los procedimientos de seguridad antes de manipular
productos químicos.
GEFAHR
Das Arbeiten mit chemischen Proben, Standards und Reagenzien ist mit Gefahren verbunden. Es
wird dem Benutzer dieser Produkte empfohlen, sich vor der Arbeit mit sicheren Verfahrensweisen
und dem richtigen Gebrauch der Chemikalien vertraut zu machen und alle entsprechenden
Materialsicherheitsdatenblätter aufmerksam zu lesen.
PERIGO
A manipulação de amostras, padrões e reagentes químicos pode ser perigosa. Reveja a folha dos
dados de segurança do material e familiarize-se com todos os procedimentos de segurança antes
de manipular quaisquer produtos químicos.
PERICOLO
La manipolazione di campioni, standard e reattivi chimici può essere pericolosa. La preghiamo di
prendere conoscenza delle Schede Techniche necessarie legate alla Sicurezza dei Materiali e di
abituarsi con tutte le procedure di sicurezza prima di manipolare ogni prodotto chimico.
1—12
1—13
Instrument Keys and Display
Item
Description
1
POWER/BACKLIGHT Key
2
ZERO/SCROLL Key
3
MENU Key
4 Numeric Display
5 Range Indicator
6 Range Indicator
7 Menu Indicator
8 Calibration Adjusted Indicator
9 Battery Low Indicator
10
READ/ENTER Key
1—14
Instrument Cap Cord
The instrument cap for the Pocket Colorimeter™ II doubles as a light shield.
Accurate measurements cannot be obtained unless the sample or blan k is covered
with the cap. Use the instrument cap cord to secure the cap to the body of the
colorimeter and prevent loss of the cap. See Figu re 1 on page 1—15.
1. Loop the instrument cap cord through the ring on the cap.
2. Remove the battery compartment cover. Press the knotted end of the cord into
the hole indicated by the arro w.
3. Slide the cord into the slot on the battery compartment cover. Snap the cover
into place.
1—15
Instrument Cap Cord, continued
Figure 1 Attaching the Instrument Cap Cord
1—16
1—17
Iron, Total (0.01 to 1.70 mg/L Fe)
Method 8 112
For water, wastewater, and seawater
TPTZ Method*
Measuring Hints
• Testing generally does not require digestion, however if the sample contains
particulate matter, digestion is required for total iron me asurem en t.**
• If samples cannot be analyzed immediately, see Sampling and Storage on
page 1—26.
• Wipe sample cells with a soft, dry cloth before placing in the instrument.
• After adding TPTZ reagent, a bl ue color will form if iron is present.
Note:
The Pocket Colorimeter™ II is designed to measure solutions contained in sample cells.
DO NOT
dip the meter in the sample or pour the sample directly into the cell holder.
* Adapted from G. Frederic Smith Chemical Co., 1980, The Iron Reagents, 3rd ed.
** See the Hach Water Analysis Handbook for further information concerning digestions.
1—18
Iron, Total, continued
Using Powder Pillows
1.Fill a 10-mL cell to the
10 mL line with sample.
2.A dd the contents of one
Iron TPTZ Reagent Powder
Pillow to the cell (the
prepared sample). Cap and
shake for 30 seconds.
3.Wait at least 3 minutes
for full color development
before completing steps 4–
12.
HRS MIN SECHRS MIN SEC
1—19
Iron, Total, continued
4.Fill a second sample cell
with 10 mL of sample (the
blank).
5.Pr ess the POWER key to
turn the meter on.
The arrow sh ould indicate
mg/L Fe.
Note:
See page 2—4 for
information on selecting the
correct range channel.
6.Wipe the sa mple cells
free of liquid or fingerprints.
Any liquid entering the
sample cell compartment
can cause damage to the
instrument.
1—20
Iron, Total, continued
7.Place the blank in the
cell holder.
8.Cov er the blank with the
instrument cap.
9.Press ZERO/SCROLL.
The display will show
“- - - -” then “0.00”.
Remove the blank from the
cell holder.
1—21
Iron, Total, continued
10. Place the prepared
sample in the cell holder.
11. Cover the sample cell
with the inst rument cap.
12.Press READ/ENTER.
The display will show
“- - - -” then “0.00”,
followed by results in mg/L
Iron.
1—22
Iron, Total, continued
Using AccuVac® Ampuls
1.Fill a 10-mL cell to the
10 mL line with sample. Cap.
Collect at least 40 mL of
sample in a 50-mL beaker.
2.Fill an Iron TPTZ
Reagent AccuVac Ampul
with sample.
Note:
Keep the tip of the
ampule immersed until the
ampule fills completely.
3.Invert the ampule several
times to mix. Wipe off any
liquid or fingerprints.
1—23
Iron, Total, continued
4.Wait at least 3 minutes
for full color development
before completing steps 5–
12.
5.Pr ess the POWER key to
turn the meter on.
The arrow sh ould indicate
mg/L Fe.
Note:
See page 2—4 for
information on selecting the
correct range channel.
6.Wipe the sa mple cells
free of liquid or fingerprints.
Any liquid entering the
sample cell compartment
can cause damage to the
instrument.
HRS MIN SECHRS MIN SEC
1—24
Iron, Total, continued
7.Place the blank in the
cell holder.
8.Cov er the blank with the
instrument cap.
9.Press ZERO/SCROLL.
The display will show
“- - - -” then “0.00”.
Remove the blank from the
cell holder.
1—25
Iron, Total, continued
10. Place the prepared
sample in the cell holder.
11. Cover the sample cell
with the inst rument cap.
12.Press READ/ENTER.
The display will show
“- - - -” then “0.00”,
followed by results in mg/L
Iron.
1—26
Iron, Total, continued
Sampling and Storage
Collect samples in acid-washed glass or plastic bottles. Adjust the sample pH to 2
or less with Nitric Acid (about 2 mL per L). Store samples preserved in this manner
up to 6 months at room temperature. If reporting only dissolved iron, filter the
sample immediately after collection and before addition of nitric acid.
Before testing, adjust the pH of the stored sample to between 3 and 4 with 5.0 N
Sodium Hydrox ide Standard Solution. Do n ot exceed pH 5 as iron may precipitate.
Accuracy Check
Standard Additions Method
1. Using a graduated cylinder, measure 25.0 mL of sample into each of three
50-mL beakers.
2. Snap the neck off an Iron Standard Solution Voluette™ Ampule, 25 mg/L Fe.
3. Use a TenSette
®
Pipet to add 0.1 , 0.2 , and 0.3 mL of standa rd, r espect ively, to
the three 50-mL beakers. Swirl to mix.
4. Analyze each standard addition sample as described in the procedure. The iron
concentration should increase by 0.1 mg/L for each 0.1 mL of standard added.
1—27
Iron, Total, continued
Note:
For analysis with powder pillows, transfer only 10 mL of solution to 10-mL sample cells.
Standard Solutions Method
Use a 1.00 mg/L Iron Standard Solution in place of the sample. Perform the Total
Iron procedure. Multiparameter standards that simulate typical drinking water
concentrations without dilution are available to confirm tests. See Optional
Reagents on page 1—31.
Standard Calibration Adjust Method
To perform a standard calibration adjustment using the 1.0 mg/L iron standard or
using an alternate concentration, see Standard Calibrat ion Adjust on page 2—13.
Method Performance
Typical Precision (95% Confidence Interval):
1.00 ± 0.02 mg/L Fe
Estimated Detection Limit:
EDL = 0.01 mg/L Fe
1—28
Iron, Total, continued
Interferences
A sample pH of less than 3 or greater than 4 after the addition of reagent ma y
inhibit color formation, caus e th e devel op e d col or to fad e qu ickl y or result in
turbidity. Before the addition of reagent, use a pH meter or pH paper to measure the
pH in the sample. A d just the sa mple pH to bet ween 3 and 8 by dr opwise ad ditio n
of an appropriate amount of iron-free acid or base such as 1.0 N Sulfuric Acid
Standard Solution or 1.0 N Sodium Hydroxide Standard Solution. Make a volume
correction if significant volumes of acid or base are used.
Interference tests were performed usi ng an iron concentra tion of 0.5 mg/L. When
interferences occurred, the color formation was inhibited or a precipitate formed.
1—29
Iron, Total, continued
The following do not interfere with the test when present up to the levels listed:
Summary of Method
The TPTZ Iron Reagent forms a deep blue-purple color with ferrous iron. The
indicator is combined with a reducing agent which converts precipitated or
suspended iron, such as rust, to the ferrous state. The amount of ferric iron present
can be determined as the difference between the results of a ferrous iron test and
the concentration of total iron.
Element Interference level Element Interference level
Cadmium 4.0 mg/L Manganese 50.0 mg/L
Chromium
(3+)
0.25 mg/L Mercury 0.4 mg/L
Chromium
(6+)
1.2 mg/L Molybdenum 4.0 mg/L
Cobalt 0.05 mg/L Nickel 1.0 mg/L
Copper 0.6 mg/L Nitrite Ion 0.8 mg/L
Cyanide 2.8 mg/L
1—30
Iron, Total, continued
Reagents and Apparatus
Required Reagents (Using Powder Pillows)
Description Quantity Pe r Test Unit Cat. No.
TPTZ Iron Reagent Powder Pillows,
10 mL size................................................2 pillows...............100/pkg ........26087-99
Required Reagents (Using AccuVac® Ampuls)
TPTZ Low Range Iron Reagent AccuVac® Ampuls
1 ampul .................25/pkg .........25100-25
Required Apparatus (Using AccuVac® Ampuls)
Description Quantity Pe r Test Unit Cat. No.
Beaker, 50 mL ................................................... 1 ...........................each..........500-41H
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