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MicroCal iTC200

GE MicroCal iTC200 Operating Instructions Manual

GE Healthcare
MicroCal™ iTC
Operating Instructions
Original Instructions
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System
Table of Contents
1 Introduction .............................................................................. 5
1.1 Important user information ................................................................................. 6
1.2 Regulatory information .........................................................................................7
1.3 Instrument ................................................................................................................ 10
1.4 Control software .................................................................................................... 13
2 Safety instructions................................................................. 15
2.1 Safety precautions ............................................................................................... 15
2.2 Labels .......................................................................................................................... 21
2.3 Recycling procedures .......................................................................................... 25
3 Installation .............................................................................. 27
3.1 Site requirements .................................................................................................. 27
3.2 Transport ...................................................................................................................29
3.3 Unpacking ................................................................................................................. 29
3.4 Set up .......................................................................................................................... 29
3.5 Validation ..................................................................................................................37
3.6 Configuring a MicroCal iTC
Table of Contents
controller for networking .................... 38
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4 Operation ................................................................................ 39
4.1 Procedure before a run ...................................................................................... 39
4.2 Basics of performing a run ............................................................................... 39
4.3 Loading the syringe ............................................................................................. 39
4.4 Loading the cell ...................................................................................................... 40
4.5 Experimental design ............................................................................................ 42
4.6 Advanced experimental design ...................................................................... 43
4.7 Instrument controls .............................................................................................. 44
4.8 Real time plot .......................................................................................................... 46
4.9 Set up .......................................................................................................................... 46
4.10 Procedures after a run ........................................................................................ 47
5 Maintenance ........................................................................... 49
5.1 Cell cleaning ............................................................................................................ 49
5.2 Removing injection syringe .............................................................................. 49
5.3 Inserting a new syringe ...................................................................................... 50
5.4 Syringe cleaning .................................................................................................... 51
5.5 Removing pipette .................................................................................................. 52
5.6 Changing pipette tips .......................................................................................... 53
5.7 Pipette calibration ................................................................................................. 54
5.8 Y-axis calibration check ..................................................................................... 54
5.9 Replacement of fuses ......................................................................................... 56
MicroCal iTC
Operating Instructions 28-9639-77 AA 3
Table of Contents
6 Troubleshooting..................................................................... 57
6.1 How to get help ......................................................................................................57
7 Reference information.......................................................... 59
7.1 Instrument specifications .................................................................................. 59
7.2 Ordering information ...........................................................................................60
4 MicroCal iTC
Operating Instructions 28-9639-77 AA
1 Introduction
Purpose of the Operating Instructions
The Operating Instructions provide you with the instructions needed to handle the MicroCal iTC
Prerequisites
In order to operate the MicroCal iTC following prerequisites must be met:
• You should have a general understanding of the use of a personal computer. running Microsoft™ Windows™ in the version provided with your product .
• You should be acquainted with the use of general laboratory equipment and with handling of biological materials.
in a safe way.
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Introduction 1
safely and according to the intended purpose the
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• You must read the Safety Instructions in Chapter 2 of these Operating Instructions.
• The system should be installed according to the instructions in Chapter 3 of these
• You should understand the concepts of titration Calorimetry.
• You must read and understand these Operating Instructions.
In this chapter
This chapter contains important user information and a general description of the MicroCal iTC
Operating Instructions.
and its intended use.
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MicroCal iTC
Operating Instructions 28-9639-77 AA 5
1 Introduction
1.1 Important user information
1.1 Important user information
Read this before using the MicroCal iTC
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All users must read the Safety Instructions in Chapter 2 of these Operating Instructions before installing, using or maintaining the system.
Intended use
Safety notices
Do not operate the MicroCal iTC documentation. If you do, you may be exposed to hazards that can lead to personal injury and you may cause damage to the equipment.
The MicroCal iTC molecular interaction studies in research applications.
The MicroCal iTC clinical procedures or for diagnostic purposes.
These Operating Instructions contain WARNINGS, CAUTIONS and NOTICES concerning the use of the product, with meanings as defined below.
is an Isothermal Titration Calorimeter system designed for bio-
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system is intended for research use only and shall not be used in any
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WARNING
WARNING indicates a hazardous situation which, if not avoided, could result in death or serious injury. It is important not to proceed until all stated conditions are met and clearly understood.
CAUTION
CAUTION indicates a hazardous situation which, if not avoided, could result in minor or moderate injury. It is important not to proceed until all stated conditions are met and clearly understood.
in any other way than described in the user
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NOTICE
NOTICE indicates instructions that must be followed to avoid damage to the product or other equipment.
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Operating Instructions 28-9639-77 AA
Notes and tips
Note: A Note is used to indicate information that is important for trouble-free and
optimal use of the product.
Tip: A tip contains useful information that can improve or optimize your procedures.
Typographical conventions
Software texts and commands are identified by bold italic text. A colon is used to separate menu levels (e.g. File:Open refers to the Open option in the File menu).
1.2 Regulatory information
This section lists the directives and standards that are fulfilled by MicroCal iTC
Manufacturing information
Introduction 1
Regulatory information 1.2
.
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CE conformity
Requirement Content
Name and address of manufacturer GE Healthcare
MicroCal Products Group 22 Industrial Drive East Northampton, Massachusetts 01060 USA
Place and date of declaration Northampton, Massachusetts,
USA, Jan. 2010
Identity of person authorized to sign Declaration of Conformity
Date of manufacture and serial number
See EC Declaration of Conformity in system documentation kit.
The serial number contains the code for the year of the manufacture of the instrument. (the serial number takes the form of) xx.yy.zzz where yy = year of manufacture.
Directive Title
2006/42/EC Machinery Directive (MD)
2006/95/EC Low Voltage Directive (LVD)
MicroCal iTC
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1 Introduction
1.2 Regulatory information
Directive Title
2004/108/EC ElectroMagnetic Compatibility (EMC) Directive
International standards
Standard Description Notes
CE marking
EN 61010-1, IEC 61010-1, CAN/CSA-C22.2
Safety requirements for electrical equipment for measurement, control and laboratory use
no. 61010-1
EN 61326-1 (CISPR Group 1, Class A)
EN-ISO 12100-1, 12100-2
EMC emissions and immunity requirements for measurement, control and laboratory use
Safety of machinery – Basic concepts, general principles and
Harmonized with 2004/108/EC
Harmonized with 2006/42/EC
design
EN-ISO 14121-1, 14121-2
Safety of machinery – Principles of risk assessment
Harmonized with 2006/42/EC
The CE marking and the corresponding Declaration of Conformity is valid for the instrument when it is:
• used as a stand-alone unit, or
• connected to other CE-marked instruments, or
• connected to other products recommended or described in the user documentation, and
• used in the same state as it was delivered from GE Healthcare, except for alterations described in the user documentation or explicitly authorized by GE Healthcare.
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Operating Instructions 28-9639-77 AA
Regulatory compliance of connected equipment
Any equipment connected to the MicroCal iTC of EN 61010-1/IEC61010-1 or relevant harmonized standards. Within the European Union, connected equipment must be CE-marked.
Introduction 1
Regulatory information 1.2
should meet the safety requirements
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MicroCal iTC
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1 Introduction
1.3 Instrument
1.3 Instrument
The MicroCal iTC heat evolved or absorbed in liquid samples as a result of mixing precise amounts of reactants. A spinning syringe is utilized for injecting and mixing of reactants. Spin rates are user selectable; the usual range is 500 to 1000 rpm. The normal temperature operating range is 2°C to 80°C. Wetted cell surfaces are Hastalloy, which are resistant to most solutions, however, strong acids should be avoided.
Sample and reference cells are accessible for filling and cleaning through the top of the unit. The sample cell is on the left as one faces the front of the unit. A pair of identical coin shaped cells is enclosed within two shields; the inner shield is referred to as the jacket. Access stems travel from the top exterior of the instrument to the cells. Both the coin shaped cells and the access stems are completely filled with liquid during operation. This requires approximately 280 µL per cell even though the working volume of the cell is only 200 µL.
(Isothermal Titration Calorimeter, 200 µL cell) unit directly measures
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Figure 1-1. Principle drawing of ITC.
Part Description Part Description
1Pipette 5Sample cell (with syringe)
2 Plunger screw (dark blue) 6 Adiabatic jackets
3 Stirring motor 7 Reference cell
4 Syringe (light blue) 8 Outer shield
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Operating Instructions 28-9639-77 AA
Introduction 1
Instrument 1.3
2
3
4
5 6
Figure 1-2. MicroCal iTC
complete system.
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1
Part Description Part Description
1 Controller 4 Washing module
2 MicroCal iTC
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5Tower
3 Pipette 6 Cell Unit
Temperature differences between the reference cell and the sample cell are measured, calibrated to power units and displayed to the user as well as saved to disk. The data channel is referred to as the DP signal, or the differential power between the reference cell and the sample cell. This signal is sometimes thought of as the "feedback" power used to maintain temperature equilibrium. Calibration of this signal is obtained electrically by administering a known quantity of power through a resistive heater element located on the cell.
In a typical experiment, the syringe containing a ligand is titrated (injected) into the cell containing a solution of macromolecule. An injection which results in the evolution of heat (exothermic) within the sample cell causes a negative change in the DP power, since the heat evolved chemically provides heat that the DP feedback is no longer required to provide.
The opposite is true for endothermic reactions. Since the DP has units of power, the time integral of the peak yields a measurement of thermal energy, dH. This heat is released or absorbed in direct proportion to the amount of binding that occurs. When the macromolecule in the cell becomes saturated with added ligand, the heat signal diminishes until only the background heat of dilution is observed.
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1 Introduction
1.3 Instrument
With the MicroCal iTC
system the entire experiment takes place under computer
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control. The user inputs the experimental parameters (temperature, number of injections, injection volumes) and the computer carries out the experiment. <DoNotTranslate_GE_Color>Origin™ software is then used to analyze the ITC data using fitting models to calculate reaction stoichiometry (n), binding constant (K
),
A
enthalpy (ΔH) and entropy (ΔS).
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Operating Instructions 28-9639-77 AA
1.4 Control software
In order for the system to initialize properly, all components must be powered up in the correct order. First, boot up the computer and log in to Windows. Once Windows has started, power the MicroCal iTC several seconds, open the MicroCal iTC copy of <DoNotTranslate_GE_Color>Origin will open automatically, as well as the MicroCal iTC
control software.
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Introduction 1
Control software 1.4
by operating the switch at the rear of the unit. After
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software. If the option is selected, a real-time
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MicroCal iTC
Figure 1-3. Instrument control software.
When the instrument first boots up, the line just below the menus reads "System Initialization - Please Wait", which is the current status of the instrument. After a few seconds, the system will begin heating or cooling to the preset temperature. If the instrument is not attached or not turned on, the program will open into Demo Mode, in which the user can see and manipulate the program, but it will not attempt to control the MicroCal iTC
. To the right of the status bar, the Time Left box, during a run, will
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show the time left until the end of the run.
When the software is first started, the Experimental Design tab is selected; this contains the simple run controls. Experimental Mode can be Highest Quality, Minimum Protein, or High Speed. The expected n, K
, and ΔH and the desired run temperature will
D
allow the software to calculate the recommended concentrations for the cell and syringe, and set the run parameters. The Advanced Experimental Design tab contains more direct controls for the more advanced user. This tab should be very familiar to users of the VP line of instruments. The Instrument Controls tab allows the user to name the output files, choose post-run analysis options, and start and stop the run. The Real Time Plot tab shows the data currently being generated. The Setup tab contains various options and preferences.
Operating Instructions 28-9639-77 AA 13
1 Introduction
1.4 Control software
Origin software
Origin Real-Time Display
This section describes the functionality of the optional copy of <DoNotTranslate_GE_Color>Origin for real-time display. When the software is opened, it will open the <DoNotTranslate_GE_Color>Origin™ project window VPITCPLOT.OPJ for real time data display. This project of <DoNotTranslate_GE_Color>Origin is dedicated to data display only, and should not be used for data analysis. Users should open a separate copy of <DoNotTranslate_GE_Color>Origin for MicroCal iTC analysis. Pictured below is the main <DoNotTranslate_GE_Color>Origin window for MicroCal iTC
data display.
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to perform data
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1
2
3
Figure 1-4. <DoNotTranslate_GE_Color>Origin Real-Time display.
No. Description
1iTC cell status
2iTC numeric display
3 Buttons for iTC data display
The ITC cell status, the MicroCal iTC
data tools (as indicated above) have been added for user convenience in viewing
iTC
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data generated by the MicroCal iTC
14 MicroCal iTC
numeric display and the buttons for MicroCal
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. For a more thorough description of these real-
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Operating Instructions 28-9639-77 AA
Introduction 1
Control software 1.4
time tools in <DoNotTranslate_GE_Color>Origin, refer to the MicroCal iTC Experimental and Data Analysis Tutorial Guide.
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MicroCal iTC
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1 Introduction
1.4 Control software
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Operating Instructions 28-9639-77 AA
2 Safety instructions
The points below are intended to enhance your safety awareness and to draw your attention to risks which only you, the operator, can prevent . While GE Healthcare works to ensure that the instrument is designed and tested to be as safe as possible, proper handling is also critical. The operators should be responsible people trained in basic laboratory protocol, and they should be familiar with the possible hazards before operating this instrument. All instrument modifications should be performed only by personnel trained by GE Healthcare. Equipment damage, personal injury or even death may result if this equipment is operated, altered or maintained by untrained personnel or in an irresponsible or improper manner.
2.1 Safety precautions
Safety instructions 2
Safety precautions 2.1
Introduction
Before installing, operating of maintaining the system, you must be aware of the hazards described in the user documentation. Follow the instructions provided to avoid personal injury or damage to the equipment.
The safety precautions in this section are grouped into the following categories:
• General precautions
• Flammable liquids
• Personal protection
• Installing and moving the instrument
•System operation
•Maintenance
MicroCal iTC
Operating Instructions 28-9639-77 AA 15
2 Safety instructions
2.1 Safety precautions
General precautions
WARNING!
Provide proper electrical power to the instrument. This should be 100 – 240 Volt, 50/60 Hertz alternating current, with a Ground Fault Circuit Interrupter (GFCI). Some power strips, such as the one provided by GE Healthcare with your instrument, contain a GFCI. All power plugs and cords should be 3-prong, grounded cables or outlets.
WARNING!
In case of fire, unplug instrument.
WARNING!
Do not operate the MicroCal iTC the MicroCal iTC
and/or Auto-MicroCal iTC
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in any other way than described in
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manuals.
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WARNING!
Make sure the rear power connector is always accessible.
WARNING!
Use caution when using solutions near the instrument. If any liquid is spilled on or around the instrument, unplug the instrument immediately and wipe it up. If there is any possibility that liquid may have leaked into the instrument case, contact GE Healthcare immediately. Do not plug the instrument into any electrical outlet until the problem is resolved.
WARNING!
This instrument is not designed to the Medical Devices Directive 93/42/ EEC and should not be used for medical purposes and/or in the diagnosis of patients.
NOTICE
The MicroCal iTC
cells are constructed out of Hastelloy. Strong acids
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should be avoided.
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Operating Instructions 28-9639-77 AA
Using flammable liquids
WARNING!
A fume hood or similar ventilation system shall be installed when flammable or noxious substances are used.
WARNING!
Fire Hazard. Before starting the system make sure that there is no leakage.
Personal protection
WARNING!
Always use protective glasses and other personal protective equipment appropriate with the current application, to ensure personal safety during operation.
Safety instructions 2
Safety precautions 2.1
WARNING!
The operator should always follow proper laboratory procedures in handling and disposing of volatile or hazardous solutions.
WARNING!
This instrument is used for a wide variety of experiments that can utilize potentially hazardous materials. Use of these could cause exposure to biological, chemical and radiation hazards depending on the user’s experiments. Users should educate themselves about the samples they are using to avoid these hazards.
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2 Safety instructions
2.1 Safety precautions
Installing and moving the instrument
WARNING!
Power cord. Only use power cords delivered or approved by GE Healthcare.
WARNING!
Do not block the ventilation inlets or outlets on the system.
WARNING!
The Washing Module may only be powered by the power supply provided with the Unit.
WARNING! Installing the controller. The controller should be installed and used
according to the instructions provided by the documentation included in the shipment.
WARNING!
Replace fuses ONLY with 4.00 Amp 250 Volt Time Delay Fuses. Several spare fuses are provided with the original shipment.
WARNING!
Access to power switch and power cord. Do not block the rear and side panel of the instrument. The Power switch must always be easy to access. The power cord must always be easy to disconnect.
NOTICE Disconnect power. To prevent equipment damage, always disconnect
power from the MicroCal iTC
and washing module system before an
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instrument module is removed or installed or a cable is connected or disconnected.
System operation
WARNING!
All solutions in the cells must be cooled down below 40ºC before removal. Any higher temperature may cause the syringe to break, and will increase the dangers of most hazardous solutions.
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Operating Instructions 28-9639-77 AA
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