GE Healthcare
Electrophoresis Power
Supply EPS 3501 XL
Operating Instructions
Original instructions
Table of Contents
1 Introduction .............................................................................. 5
1.1 Important user information .................................................................................5
1.2 Regulatory information ......................................................................................... 7
1.3 Instrument ................................................................................................................... 9
2 Safety instructions................................................................. 15
2.1 Safety precautions ............................................................................................... 15
2.2 Labels .......................................................................................................................... 18
2.3 Emergency procedures ...................................................................................... 20
2.4 Built-in safety features ....................................................................................... 20
2.5 Recycling information ......................................................................................... 21
3 Installation .............................................................................. 23
3.1 Site requirements .................................................................................................. 23
3.2 Unpacking ................................................................................................................. 23
3.3 Transport ...................................................................................................................23
3.4 Connections ............................................................................................................. 24
3.5 Spare parts and accessories ........................................................................... 25
Table of Contents
4 Operation ................................................................................ 27
4.1 Operation overview .............................................................................................. 27
4.2 Starting the instrument ...................................................................................... 27
4.3 Setting up a run ..................................................................................................... 28
4.4 Preparations before start .................................................................................. 35
4.5 Performing a run ................................................................................................... 35
5 Maintenance ........................................................................... 41
5.1 General ....................................................................................................................... 41
6 Troubleshooting ..................................................................... 43
7 Reference information .......................................................... 45
7.1 Specifications .......................................................................................................... 45
7.2 Literature ................................................................................................................... 45
7.3 Ordering information ........................................................................................... 45
Appendix A Electrical and communication connections...... 47
Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA 3
Table of Contents
4 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA
1 Introduction
Purpose of the Operating
Instructions
The Operating Instructions provide you with the instructions needed to handle
Electrophoresis Power Supply EPS 3501 XL in a safe way.
Prerequisites
In order to operate EPS 3501 XL safely and according to the intended purpose the
following prerequisites must be met:
• You should be acquainted with the use of general laboratory equipment and with
handling of biological materials.
• You should understand the concepts of electrophoresis.
Introduction 1
Important user information 1.1
• You must read the Safety Instructions in Chapter 2 of these Operating Instructions.
• The system should be installed according to the instructions in Chapter 3 of these
Operating Instructions.
In this chapter
This chapter contains important user information and a general description of EPS 3501
XL and its intended use.
1.1 Important user information
Read this before using EPS 3501
XL
All users must read the Safety Instructions in Chapter 2 of these Operating Instructions
before installing, using or maintaining the instrument.
Do not operate EPS 3501 XL in any other way than described in the user documentation.
If you do, you may be exposed to hazards that can lead to personal injury and you may
cause damage to the equipment.
Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA 5
1 Introduction
1.1 Important user information
Intended use
EPS 3501 XL is a high quality, high precision power supply for electrophoresis
applications that require advanced programming and high voltage.
EPS 3501 XL is intended for research use only, and shall not be used in any clinical
procedures, or for diagnostic purposes.
Safety notices
These Operating Instructions contain WARNINGS, CAUTIONS and NOTICES concerning
the use of the product, with meanings as defined below.
WARNING
WARNING indicates a hazardous situation which, if not avoided, could
result in death or serious injury. It is important not to proceed until all
stated conditions are met and clearly understood.
CAUTION
CAUTION indicates a hazardous situation which, if not avoided, could
result in minor or moderate injury. It is important not to proceed until all
stated conditions are met and clearly understood.
NOTICE
NOTICE indicates instructions that must be followed to avoid damage to
the product or other equipment.
6 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA
Notes and tips
Note: A Note is used to indicate information that is important for trouble-free and
optimal use of the product.
Tip: A tip contains useful information that can improve or optimize your procedures.
Typographical conventions
Software texts and commands are identified by bold italic text. A colon is used to
separate menu levels (e.g. File:Open refers to the Open option in the File menu).
1.2 Regulatory information
This section lists the directives and standards that are fulfilled by EPS 3501 XL.
Manufacturing information
Introduction 1
Regulatory information 1.2
CE Conformity
Requirement Content
Name and address of manufacturer GE Healthcare Bio-Sciences AB,
Björkgatan 30, SE 751 84 Uppsala
Sweden
Name and ID of notified body INTERTEK SEMKO AB, NB 0413
Place and date of declaration Uppsala, Sweden, May 2009
Identity of person authorized to sign DoC See EC Declaration of Conformity
Directive Title
2006/42/EC Machinery Directive (MD)
2006/95/EC Low Voltage Directive (LVD)
2004/108/EC ElectroMagnetic Compatibility (EMC) Directive
Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA 7
1 Introduction
1.2 Regulatory information
International standards
Standard Description Notes
CE marking
EN 61010-1,
IEC 61010-1,
CAN/CSA-C22.2
No. 61010-1
EN 61326-1 EMC emissions and immunity
EN-ISO 12100-1,
12100-2
EN-ISO 14121-1,
14121-2
The CE marking and the corresponding Declaration of Conformity is valid for the
instrument when it is:
Safety requirements for electrical
equipment for measurement, control
and laboratory use
requirements for electrical equipment
measurement, control and laboratory
use
Safety of machinery – Basic concepts,
general principles and design
Safety of machinery – Principles of risk
assessment
Harmonized with
2004/108/EC
Harmonized with
2006/42/EC
Harmonized with
2006/42/EC
• used as a stand-alone unit, or
• connected to other CE-marked instruments, or
• connected to other products recommended or described in the user
documentation, and
• used in the same state as it was delivered from GE Healthcare, except for
alterations described in the user documentation or explicitly authorized by
GE Healthcare.
Regulatory compliance of
connected equipment
Any equipment connected to EPS 3501 XL should meet the safety requirements of EN
61010-1/IEC61010-1 or relevant harmonized standards. Within the European Union,
connected equipment must be CE-marked.
8 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA
1.3 Instrument
This chapter contains a general description of EPS 3501 XL.
General description
EPS 3501 XL is a high quality, high precision power supply for electrophoresis
applications that require advanced programming and high voltage.
EPS 3501 XL is primarily designed for techniques using programming in several phases
and/or voltage gradients:
• 2-D (Two dimensional) electrophoresis using Multiphor II Electrophoresis System
• IEF (Isoelectric focusing) using Immobiline™ or carrier ampholytes such as
CleanGel™ and ExcelGel™
• Flatbed SDS-PAGE (Polyacrylamide Gel Electrophoresis) or flatbed native PAGE
EPS 3501 XL is also suitable for:
•DNA sequencing
Introduction 1
Instrument 1.3
• Agarose electrophoresis
•Electroblotting
• DNA pulsed field electrophoresis
Nine programs each with up to nine phases can be saved. Limiting values for voltage,
current and power as well as voltage gradients can be programmed for precise control
of the electrophoresis. The EPS 3501 XL automatically switches over the controlling
parameter according to programmed limits and gradients and conductivity variations
in the system.
Two electrophoresis units can be connected to EPS 3501 XL and run with the same
programmed method at one time.
Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA 9
1 Introduction
1
3
2
1.3 Instrument
Instrument description
Figure 1-1. The main parts of the EPS 3501 XL instrument.
Part Description
Display
1
Keyboard
2
Output sockets
3
10 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA
Indicator and switch on the instrument unit
HV on
ST OP
pa
con
delete
MAINS
Disconnect before serv
I
0
1
4
2
3
5
8
6
7
Indicator/Switch Color Description
Introduction 1
Instrument 1.3
Front panel
HV ON
(indicator lamp)
Power
(switch)
Green The HV ON lamp indicates that the
high voltage power supply is on.
- Switches on/off power to the
instrument. Located on the rear
panel.
The front panel consists of an alphanumeric display, a keyboard with 9 membrane keys,
a light emitting diode (LED) that lights when voltage is applied (HV on) and connectors
for two electrophoresis units.
Display
Part Description Part Description
1
2
3
Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA 11
4
Mode
Voltage
Current
Power
Breakpoint parameter
5
Phase number
6
Control mode (step)
7
Program number
8
1 Introduction
ent er
set
ru n
pause
continue
ST OP
1.3 Instrument
Keyboard
Key Label Description
set enter Press set enter to enter a value or choice, confirm this if
valid, and move programming to the next field. Valid
values are voltage 35–3 500 V, current 1–400 mA, power
1–200 W, time 0:01–500 h, volthours 1–500 000 Vh,
milliampere-hours 1–25 000 mAh.
In the RUN mode, pressing set enter shows the
programmed parameters for the actual run. In addition,
set enter allows you to make changes in the program
during a run after first pressing pause continue.
After a run, when in END mode, pressing set enter puts
the instrument into SET, its programming mode.
- Press to change the parameter, value or other
choice in the field which is blinking. Numerical values are
changed in an accelerating manner when a key is held
down. Clicking a key changes the value in preset
increments. Parameters or units (e.g. Vh) and choices (e.g.
Yes/No) are changed with one key push. The keys can also
be used to switch between time and volthours in RUN,
PAUSE and END. The values scroll i.e. they automatically
change from maximum to minimum value or vice versa.
run Press run to start the run and put the program into RUN
mode. The current values for voltage, current and power
are shown on the display. The elapsed time, volthours or
milliamperehours are also displayed. Switch between
these last three parameters with
pause
continue
Press pause continue to put the instrument in PAUSE
mode and switch off the voltage. The display shows the
status of the run at the time the key was pressed. pause
continue only operates in RUN mode. Time, integrated
voltage and integrated current are retained.
In the PAUSE MODE mode, set enter can be used to make
changes in the program. Return to RUN mode by pressing
pause continue or by pressing run.
STOP Press STOP to stop the run and put the instrument in END
mode. The voltage is switched off and the end parameters
are displayed. Switch between time, integrated voltage
and integrated current by pressing . A run cannot be
continued after pressing STOP. Press run to run the same
method again or press set enter to choose another
program, program a new method or make changes in an
existing method.
12 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA
Key Label Description
+
-
Introduction 1
Instrument 1.3
Inser t
delet e
mo re
exit
insert
delete
Press insert delete to insert or delete a phase in a
program. This function is activated in SET mode. Note that
a program must be completed by answering Yes (Y) to the
question “Last Phase?” in SET mode or by pressing exit
before you can use the insert delete key.
more Press more to place program in MORE mode. This gives
access to some special functions. These include:
COPY: Copying a program.
CLEAR: Clearing a program.
SETUP: Disabling the start current check.
MORE mode cannot be activated in RUN or PAUSE mode.
Leave MORE by pressing exit.
exit Press exit to stop the execution of an operation, such as
the entry of a value. Only values/units that have already
been confirmed by set enter are retained when exit is
pressed. Note that if a phase contains zeros when pressing
exit, that phase will be deleted.
Press exit to return the instrument to the mode that was
left or to the start position in SET.
Output sockets
There are two sets of output sockets to allow two electrophoresis units to be connected
and run at the same time.
Output socket Label Color Voltage
Positive + Red 0 and +1750 V
Negative - Black or blue 0 and –1750 V
Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA 13
1 Introduction
1.3 Instrument
14 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA
2 Safety instructions
This chapter describes safety compliance, safety labels, general safety precautions,
emergency procedures, power failure and recycling of EPS 3501 XL.
2.1 Safety precautions
Introduction
The EPS 3501 XL is powered by mains voltage and handles liquids that may be
hazardous. Before installing, operating or maintaining the equipment, you must be
aware of the hazards described in this manual. Follow the instructions provided to avoid
personal injury or damage to the equipment.
Safety instructions 2
Safety precautions 2.1
The safety precautions in this section are grouped into the following categories:
• General precautions
• Personal protection
• Installing and moving the instrument
•System operation
•Maintenance
General precautions
WARNING
Do not operate the EPS 3501 XL in any other way than described in the
EPS 3501 XL User Manual.
WARNING
Operation and user maintenance of EPS 3501 XL should be performed
by properly trained personnel only.
Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA 15
WARNING
Do not use any accessories not supplied or recommended by
GE Healthcare.
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