GE EPS 301 User Manual
Electrophoresis Power Supply
EPS 301
User Manual
Manuel d’Utilization
Bedienungsanleitung
Manual del usuario
Manuale dell’operatore
18-1130-18
Edition AB
Electrophoresis Power Supply - EPS 301
Fig. 1. The front panel of the EPS 301.
Figure 1. Le panneau avant de l’EPS 301.
Abb. 1. Die Fronttafel des EPS 301.
Figura 1. Panel frontal de la EPS 301.
Figura 1. Pannello anteriore dell’EPS 301.
Fig. 2. The rear panel of the EPS 301.
Figure 2. Le panneau arrière de l’EPS 301.
Abb. 2. Die Rückwand des EPS 301.
Figura 2. Panel posterior de la EPS 301.
Figura 2. Pannello posteriore dell’EPS 301.
Voltage 100-120/220-240 V AC
Frequency 50/60 Hz
Power 120 W 1.5 A Max
MAINS
Disconnect before servicing
HV on
run
STOP
view
set
enter
I
0
100-120 220-240
1
1. Introduction. . . . . . . . . . . . . . . . . . . . . . 2
2. Safety information . . . . . . . . . . . . . . . . . 2
2.1 Safety precautions . . . . . . . . . . . . . . 2
2.2 Built-in safety features . . . . . . . . . . . 2
3. Unpacking and installation. . . . . . . . . . . 3
4. Technical description . . . . . . . . . . . . . . . 4
4.1 Materials . . . . . . . . . . . . . . . . . . . . . 4
4.2 Front panel . . . . . . . . . . . . . . . . . . . 4
4.3 Display. . . . . . . . . . . . . . . . . . . . . . . 4
4.4 Keyboard. . . . . . . . . . . . . . . . . . . . . 4
4.5 Output sockets. . . . . . . . . . . . . . . . . 5
4.6 Rear panel . . . . . . . . . . . . . . . . . . . . 5
5. Operation. . . . . . . . . . . . . . . . . . . . . . . . 5
5.1 Connecting the . . . . . . . . . . . . . . . . .
electrophoresis unit(s) . . . . . . . . . . . 5
5.2 Programming. . . . . . . . . . . . . . . . . . 5
5.3 Running. . . . . . . . . . . . . . . . . . . . . . 5
5.4 Short instructions. . . . . . . . . . . . . . . 6
6. Maintenance . . . . . . . . . . . . . . . . . . . . . 8
7. Trouble shooting . . . . . . . . . . . . . . . . . . 8
8. Technical specifications . . . . . . . . . . . . . 9
9. Ordering information. . . . . . . . . . . . . . . 9
Contents
EPS 301 - User Manual
Please read this entire manual to
fully understand the safe use of
EPS 301
WARNING!
The Warning sign
highlights an instruction
that must be strictly
followed in order to
avoid personal injury. Be sure not to
proceeed until the instructions are
clearly understood and all stated
conditions are met.
Declaration of conformity
Safety Standards
This product meets the requirement
of the Low Voltage Directive (LVD)
73/23/EEC through the harmonized
standard EN 61010-1, 1993+ A1,
1992.
Important user information
!
EMC Standards
This product meets the requirement
of the EMC Directive 89/336/EEC
through the harmonized standards
EN 50081-1 (emission) and EN
50082-1 (immunity).
The CE symbol, and corresponding
declaration of conformity, is valid
for the instrument when it is.
– used as a “stand alone” unit or
– connected to other CE marked
GE Healthcare
instruments, or
– connected to other products
recommended or described in
this manual and
– is used in the same state as it was
delivered from GE Healthcare
except for alterations
described in this manual.
Terms and Conditions of Sale
All goods and services are sold
subject to the terms and conditions
of sale of the company within the
General Electric Company group which
supplies them. A copy of these terms
and conditions is available on
request.
Should you have any comments on
this product, we will be pleased to
receive them at:
Hoefer Biosciences Inc.
654 Minnesota Street
San Francisco, CA 94107 USA
Trademarks
GE Healthcare is a trademark of
General Electric Company.
Pharmacia and Drop Design are
trademarks of Pharmacia & Upjohn
Inc.
Office Addresses
GE Healthcare Bio-Sciences AB
SE-751 84 Uppsala
Sweden
GE Healthcare UK Ltd
GE Healthcare Place Little Chalfont
Buckinghamshire
England HP7 9NA
GE Healthcare Bio-Sciences Inc.
800 Centennial Avenue
P.O. Box 1327
Piscataway N.J. 08855-1327
USA
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EPS 301 - User Manual
1. Introduction
The GE Healthcare Electrophoresis Power Supply EPS 301 is a high quality, high precision and
safe power supply for electrophoresis applications that require a maximum of 300 volts, 400 mA and 80
W.
EPS 301 is primarily designed for the following techniques:
1. Submarine DNA electrophoresis
2. Electroblotting
3. Immuno electrophoresis
4. Mini polyacrylamide gel electrophoresis
Electrophoresis separations can be controlled by voltage or current. The EPS 301 automatically switches
over the controlling parameter according to programmed limits and conductivity variations in the
system.
Two electrophoresis units can be connected to the EPS 301 and run with the same programmed method
at one time.
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2. Safety information
2.1 Safety precautcions
Because this instrument can develop sufficient voltage and current to produce a lethal shock, extreme
caution should be exercised in its operation.
The power supply should only be used by properly trained operators. Read this entire manual before
using this power supply and use only according to the manufacturer’s instructions.
1. This instrument is designed for indoor use only.
2. The instrument must always be used with the earth lead of the power cord correctly grounded to
earth at the mains outlet.
3. To permit sufficient cooling, ensure that the vents in the rear and sides of the instrument are not
covered.
4. Do not operate the instrument in extreme humidity (above 95%). Avoid condensation by letting the
unit equilibrate to ambient temperature when taking the power supply from a colder to a warmer
environment.
5. Keep the instrument as dry and clean as possible. Wipe regularly with a soft damp cloth. Let the
power supply dry completely before use. If wetted, unplug the power supply until the instrument is
dry.
6. Use only undamaged electrical wire and equipment specified for the voltages you will use. High
voltage electrical wires should be in accordance with IEC 1010-2-031:1993. All equipment connected
to high voltage should be in accordance with IEC 1010-1:1993.
2.2 Built-in safety features
The EPS 301 has been tested and complies with the IEC 61010-1 (EN 61010-1) electrical safety
standard.
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EPS 301 - User Manual
3. Unpacking and installation
Unpacking
Check the contents against the packing list supplied. Inspect for any damage that may have occurred
during transit. Report any damage immediately to your local GE Healthcare representative and
to the transport company concerned.
Mains connection
Select the appropriate voltage range, 100-120 or 220-240 V. See Fig. 2 inside front cover.
Warning! If the power supply is connected to 220-240 V with the range set to 100-120 V, the
instrument can be severely damaged.
Select the appropriate mains cable and connect one end to the mains socket on the EPS 301 power
supply, see Fig. 2 inside front cover, and the other end to an AC grounded outlet.
Switch on the power. Each time the instrument is turned on a self diagnostic test is done. If an error is
detected during the test a message will appear on the display and an alarm will sound.
w
Connection of the electrophoresis unit(s)
Connect the leads from the electrophoresis unit (red to red, and black or blue to blue). The red lead is
the positive and black or blue is the negative.
Warning! Use only undamaged electrical cables and equipment approved for the voltage you will use.
High voltage electrical cables should be in accordande with IEC 1010-2-031:1993. All equipment
connected to high voltage electrical cables should be in accordance with IEC 1010-1:1993.
Local regulation for Great Britain
IMPORTANT WARNING
This appliance must be earthed.
The wires in the mains lead are coloured in accordance with the following code:
Green and yellow Earth
Blue Neutral
Brown Live
If the plug provided is unsuitable for your socket outlets, the plug must be cut off and a suitable plug
fitted. The cut-off plug should be disposed of and must not be inserted into any socket as this can
result in electric shock. The plug or adapter or the distribution panel should be provided with a 13
amp fuse. As the colours of the wires in the mains lead of this appliance may not correspond with
coloured markings identifying the terminals in your plug, proceed as follows:
The green and yellow wire must be connected to the terminal in the plug which is marked with the
letter E or by the earth symbol, or coloured green, or green and yellow.
The blue wire must be connected to the terminal which is marked with the letter N or coloured black.
The brown wire must be connected to the terminal which is marked with the letter L or coloured red.
NOTE After replacing or changing a fuse, the fuse cover in the plug must be replaced with a fuse
cover which corresponds to the colour of the insert in the base of the plug or the word that is
embossed on the base of the plug, and the appliance must not be used without a fuse cover.
Only 13 Amp fuses approved to B.S. 1362 A.S.T.A. should be used.
3
4
4. Technical description
4.1 Materials
The main components in the EPS 301 power supply are made of the following materials.
Instrument box Aluminium
Display holder Polypropene (PP). UL94V0
Keyboard Polyester
Output sockets Acetalplastic (POM). UL94V0
Rubber feet Polyurethane
4.2 Front panel
On the front panel there is a numerical display illustrated with the symbols, V, mA, and h.m., a
keyboard with six membrane keys, a light emitting diode that lights when voltage is applied (HV on) and
output sockets for two electrophoresis units.
4.3 Display
A four digit numeric display guides you through the programming, shows actual parameter values during
the electrophoresis and final parameter values afterwards. It also shows error messages.
Figure 1, inside front cover, shows the display in the End position when power is switched on.
4.4 Keyboards
SET/ENTER
Puts the instrument into its program mode. Pressing SET/ENTER in the program mode enters a value, if
valid, and moves the program to the next parameter.
In the RUN mode, pressing SET/ENTER allows you to make changes in the program during a run.
Valid values are: voltage 5-300 V, current 10-400 mA, time 0.01-23.59 h, or timer off - - - -.
UP/DOWN
Changes the parameter value V, mA, and h.m. when the display is flashing. Numerical values are
changed in an accelerating manner when the key is held down. Clicking / changes the value in pre-set
increments. The values will automatically change from maximum to minimum value or vice versa, except
for the time (h.m.) where the signs - - - - are placed between the minimum and maximum values
indicating that the timer is switched off.
RUN
Starts the run and puts the program into RUN mode. The light (HV on) is switched on.
VIEW
In run mode, VIEW switches between the actual values for voltage, current and elapsed time. Pressing
VIEW for more than three seconds starts automatic switching between the actual values for voltage,
current and elapsed time. This automatic switching is stopped by pressing VIEW or RUN.
In End mode, VIEW switches between the end parameter values.
STOP
Stops the run and puts the instrument in End mode. The voltage and the light (HV on) are switched off.
By pressing VIEW the end parameter values are displayed.
view
/
EPS 301 - User Manual
set
enter
run
STOP
5
4.5 Output sockets
There are two sets of output sockets connected in parallel to allow two electrophoresis units to be
connected and run at the same time, see Fig. 1 inside front cover.
4.6 Rear panel
The rear panel is shown in Fig. 2, see inside front cover. On the rear panel there is:
1. A mains switch. Press in I to switch on the power to the power supply. Press 0 to switch off the
power.
2. A socket for the mains cable.
3. A switch for voltage range. The left position corresponds to 100–120 V and the right to 220–240 V.
4. Vents.
5. Operation
5.1 Connecting the electrophoresis unit(s)
Connect the leads from the electrophoresis unit (red to red, and black or blue to blue). Red is positive
and black or blue negative. Two electrophoresis units can be run at the same voltage simultaneously.
When two electrophoresis units are run at the same time, double the current. Voltage will be the same
whether one or two units are run.
5.2 Programming
Programming
Enter the program mode by pressing SET/ENTER. When the display is flashing and one of the
parameters V, mA or h.m. is lit, the program is ready for setting the values by using the / buttons.
Confirm and go to the next parameter by pressing SET/ENTER.
Pressing STOP in program mode saves the displayed value and puts the program in End mode. When the
EPS 301 power supply is switched on, the programmed values from the last run can be used directly by
pressing run.
Set limits for voltage, current and time
The flashing display and the lighted symbol V indicates that the voltage limit can be set. Using the /
buttons, select the maximum voltage desired for the run. Confirm with SET/ENTER. Repeat the same
procedure to set current and time limits. If no time limit is used, switch the timer off by setting the time
to zero. This is indicated by - - - -. Confirm with SET/ENTER. Programmable values are: voltage, 5-300
V, current, 10-400 mA, time, 0.01-23.59 h or timer off (- - - -).
Alarm
When the programmed time has elapsed, the program will enter the End mode and the alarm will sound.
You can stop the alarm by pressing any key. The timer and alarm are switched off by adjusting the time
to zero.
5.3 Running
Run
Press RUN to start the electrophoresis. A light emitting diode shows when voltage is applied (HV on).
The display will show one of the actual values. If no current is displayed, please check the electrical
connections to the electrophoresis equipment.
EPS 301 - User Manual
6
View actual values
Press VIEW in Run mode to change between the actual values for voltage, current or elapsed time. Press
VIEW for more than three seconds to switch automatically between these values. Stop the automatic
switching by pressing VIEW or RUN.
View programmed values
Press SET/ENTER to view the programmed values during a run. If the keyboard is idle for 4 seconds or
you press RUN the display goes back to the actual values.
Change parameters during a run
When the method is running, changes can be introduced in the program by pressing SET/ENTER and
using /. Confirm by pressing SET/ENTER or RUN
Stop the run
When the programmed time has elapsed, the program will enter the End mode and the alarm will sound.
You can stop the alarm by pressing any key. It is also possible to break the run manually by pressing
STOP. In both cases, the values for voltage and current will go to zero, as indicated by the light emitting
diode switching off.
View end parameter values
Display the end parameter values by pressing VIEW.
Post electrophoresis
Disconnect the leads and proceed with post-electrophoretic techniques. Since the quality of your
separation will deteriorate due to diffusion, you should remove the gel and begin staining or blotting
immediately.
5.4 Short instructions
This section summarises the main points covered earlier in this chapter. Use it as a check list once you
are familiar with the detailed programming and running instructions. Refer also to the separate short
instruction EPS 301 included with the power supply. We recommend you keep this separate short
instruction close by the instrument.
1. Turn mains power ON. The program enters the End mode. If the programmed values from the last
run are to be used again, go to point 4 below. To change the program, press SET/ENTER to enter the
program mode. The voltage symbol V is lit and the value for voltage flashes. The programmed value
from the last run is displayed. Use / to set to the desired voltage. Press SET/ENTER to confirm.
If the voltage value is correct from the beginning just press SET/ENTER to confirm.
2. The display shows the last programmed value for current flashing. Press / until the desired value
is reached. Confirm by pressing SET/ENTER.
3. The value for time is now flashing. Use / to set the time. To switch off the timer, set the time to
zero. Indicated by - - - -. Confirm by pressing SET/ENTER.
4. When programming is completed, connect your electrophoresis unit to the output(s), and press RUN.
5. Press VIEW to display actual voltage, current and elapsed time. Press VIEW for more than three
seconds to start automatic switching of actual values.
6. Press SET/ENTER during the run to check the programmed parameters and change the values by
pressing /. Confirm by SET/ENTER.
7. The program stops automatically on completion or on STOP being pressed.
8. Press VIEW after the run to check the end parameters.
EPS 301 - User Manual
7
EPS 301 - User Manual
Fig. 3. Step-by-step summary of programming and running.
run
set
enter
set
enter
set
enter
set
enter
view
view
view
view
STOP
view
view
view
8
-PF-
FAIL
EPS 301 - User Manual
6. Maintenance
Wipe the instrument regularly with a damp cloth. Let the instrument dry completely before use. All
servicing should be entrusted to qualified personnel only. Please contact your local GE Healthcare
representative for more service information.
7. Trouble shooting
If an error occurs, either during a run or when switching on the power supply, the output is switched off
if necessary and an error message will be displayed. The following messages can be displayed.
Mains power failure
The program continues when power is resumed. The display switches between PF and the actual
parameter value. The message -PF- is removed by pressing any key. No special action is needed.
Serious error
If a serious error occurs, the program enters the FAIL mode. The output is switched off and FAIL is
shown in the display.
Please contact your GE Healthcare representative.
9
8. Technical specifications
Regulation Maximum voltage and current with automatic cross-over at preset limits
Output mode Contante Voltage: 0-300 V DC,
Contante Current: 0-400 mA
Maximum Power Output 80 W
Programming range Voltage: 5-300 V DC
Current: 10-400 mA
Time: 00.01-23.59 h or off (- - - -)
Output resolution Voltage: 1 V
Current: 1 mA
Programming resolution Voltage: 1 V
Current: 1 mA
Time: 1 min
Accuracy Voltage: 4%, ± 2 V
Current: 4%, ± 4 mA
Timer: 0.1% ± 1 min
Line regulation < 0.5%
Load regulation < 1% at load change 10-90% of maximum load
Ripple < 3% at 300 V
Short term stability < 0.2% /10 h after warm up
Long term stability < 1% /year
Output protection Fully protected against any overload conditions
Recovery after power failure The program continues automatically
Ambient operating temperature 4-40 °C
Ambient operating humidity 0-95%
Ambient operating pressure 68-106 kPa, maximum altitude of 2000 m
Mains requirements 100-120 V/220-240 V; 50/60 Hz
Power consumption Max 120 W
Dimensions (WxDxH) 250 x 215 x 95 mm
Weight 3.0 kg
9. Ordering information
Designation Code No.
Electrophoresis Power Supply - EPS 301 18-1130-01
EPS 301 - User Manual
10
EPS 301 - User Manual
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