Dynex MW 91000051 User Manual

Opsys MW

Please read carefully before using the Opsys MW

™

Microplate Washer

User’s Guide

IMPORTANT

Part No. 91000051, Revision B This manual is published by DYNEX Technologies, Inc. Questions or comments regarding the content of this manual can be

directed to the address below or to your DYNEX Technologies representative.

DYNEX Technologies 14340 Sullyfield Circle Chantilly, VA 20151-1683 USA

Tel. (703) 631-7800

(800) 288-2354

Fax. (703) 803-1441

OPSYS MW

is a trademark of DYNEX Technologies, Inc.

DYNEX Technologies reserves the right to make technical improvements to this equipment and documentation without prior notice as part of a continuous program of product development. This manual supersedes all previous editions.

Warranty and Special Provisions Limited Warranty

DYNEX Technologies products are fully guaranteed for one year against defects in parts, materials, and workmanship. Defective parts and materials will be replaced or, at the discretion of DYNEX Technologies, repaired at no charge for a period of one year and labor required for such replacement or repair will be provided at no charge for a period of one year, provided that the products are utilized and maintained in accordance with the instructions in the applicable operating and servicing manual, and provided further that the products have not, as determined solely by DYNEX Technologies, been subject to misuse or abuse by the Customer or other parties unrelated to DYNEX Technologies. DYNEX Technologies makes no warranty, expressed or implied, as to the fitness of any product for any particular purposes other than those purposes described in the applicable operating and servicing manual, nor does DYNEX Technologies make any other warranty, whether expressed or implied, including merchantability, other than those appearing on the face hereof. Where DYNEX Technologies guarantees any product, whether under this Warranty or as a matter of law, and there is a breach of such guarantee, the Customer’s only and exclusive remedy shall be the replacement or repair of defective parts and materials, as described above. This shall be the limit of DYNEX Technologies’ liability. Furthermore, DYNEX Technologies shall not be liable for incidental or consequential damages. Failure of the Customer to notify DYNEX Technologies of a claimed defect by registered mail within thirty days of the discovery thereof shall constitute a waiver of any claim for breach of warranty.

When a product is required by DYNEX Technologies to be installed by a DYNEX Technologies engineer or technician, the period of this Warranty shall begin on the date of such installation, provided however, that any use of the product prior to such installation shall, at sole election of DYNEX Technologies, void this Warranty. When installation by DYNEX Technologies personnel is not required, the period of this Warranty shall begin on the date of shipment from DYNEX Technologies. The period of this Warranty shall begin as described above whether or not the product has been installed pursuant to a purchase order, and any trial period shall be deducted from the Warranty period that would otherwise apply under a subsequent placed purchase order for that product.

Limitation of Liability. Notwithstanding anything to the contrary contained herein, the liability of Seller (whether by reason of breach of warranty, breach of contract, tort, or otherwise), including without limitation under any indemnification provision contained herein, shall be limited to replacement of goods returned to DYNEX Technologies which are shown to DYNEX Technologies’ reasonable satisfaction to have been nonconforming or to refund the purchase price, or, if not paid, to a credit amount of the purchase price therefore.

THE FOREGOING WARRANTIES ARE EXCLUSIVE AND ARE GIVEN AND ACCEPTED IN LIEU OF ANY AND ALL OTHER WARRANTIES, EXPRESS OR IMPLIED, INCLUDING WITHOUT LIMITATION, THE IMPLIED WARRANTY OF MECHANTABILITY AND THE IMPLIED WARRANTY OF FITNESS FOR A PARTICULAR PURPOSE. NEITHER PARTY SHALL BE LIABLE TO THE OTHER FOR ANY INCIDENTAL, INDIRECT, SPECIAL, OR CONSEQUENTIAL DAMAGES.

Table of Contents

About this Manual iii Chapter 1 Introduction 1

Description 1

Programming 2 Wash Head 3 Wash Head Positions 4 Wash Fluid 5 Waste 5

Features 7 Specifications 9 Warning Labels 11

Chapter 2 Installation 13

Unpacking 13

Materials Provided 13

Hardware Components 15 Positioning the Instrument 18 Installing the Wash Head 19 Installing the Wash Container 20 Installing the Waste Container 21 Turning on the System 22

Chapter 3 Configuration 23

The Keypad 23 Menus 26

The SETUP Menu 27 The PROGRAM Menu 27

Configuring the System 28

Specifying the Wash Head 29 Specifying Plate Parameters 30 Displaying the Software Version 35 Specifying the Pump Parameters 36 Selecting Clean 37

Chapter 4 Wash Protocols 39

Wash Protocol Operations 39 Creating a New Wash Protocol 41

Specifying the Header 41 Specifying Wash Cycle Operations 45 Ending a MOVE Cycle 53

Modifying a Wash Protocol 56 Copying a Wash Protocol 61

Chapter 5 Washing a Plate 63

Preparation 63 Washing a Plate 65

Chapter 6 Routine Maintenance 67

Routine Maintenance Procedures 67

Opsys MW™ User’s Guide i

Table of Contents

Cleaning and Decontamination 69 Cleaning the Containers 70 Replacing the Tubing 71 Pump Calibration 74

Chapter 7 Troubleshooting 77

Operational Problems 77

Chapter 8 Service 81

Service Procedures 81 Spare Parts 82 Returning a Washer for Service 83 Contact DYNEX Technologies 85

Index 86

ii Opsys MW™ User’s Guide

About this Manual

This manual has been written for laboratory technicians and provides detailed instructions for using the Opsys MW™ system.

This manual gives you the information needed to:

• Review safety precautions.

• Install the Opsys MW™ system.

• Understand the Opsys MW™ menus.

• Set the Opsys MW™ system up to suit your exact needs.

• Create or modify wash protocols.

• Run wash protocols using the Opsys MW™ system.

• Perform required preventive maintenance.

• Troubleshoot the system.

Opsys MW™ User’s Guide iii

About this Manual

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iv Opsys MW™ User’s Guide

Description

Chapter 1 Introduction

The Opsys MW™ is a microprocessor-controlled microplate washing system that performs wash protocols that are defined by the user. It is designed to wash all of the wells in one column or one row of a 96-well plate at once. The washing protocol can be programmed so that all of the columns (or rows) are washed in the same manner, or different wash cycles can be applied to specified columns (or rows) on the plate.

Description

The Opsys MW Microplate Washer is shown in Figure 1.

Figure 1. The Opsys MW™ Microplate Washing System

Opsys MW™ User’s Guide 1

Description

Programming

All wash protocols on the Opsys MW™ Microplate Washer are user-defined. Up to 40 wash protocols can be contained on the system.

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In addition, the system can be configured with up to ten different plate types so that the wash head positions for each plate type can be specified.

Programming of a new wash protocol (or modifying an existing one) and configuration of the system are carried out using the keypad and display.

Note: Memory limitations may restrict the size and complexity of an individual wash protocol.

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Description

Wash Head

The wash head on the Opsys MW™ Microplate Washer contains multiple sets of dual wash pins. Each set of dual wash pins consists of a dispense pin and an aspirate pin that are closely spaced so that fluid can be aspirated from and dispensed into a well simultaneously.

The following wash heads can be fitted on the Opsys MW™:

Wash Head Description

1 x 8

Eight sets of dual wash pins for washing a 96-well microplate one column (8 wells) at a time.

1 x 12

If a wash head is installed to wash plates by columns, the plates are placed on the instrument so that the columns of the plate are aligned with the wash head. If a wash head is installed to wash plates by row, the plate is rotated 90 degrees and placed on the instrument so that the rows of the plate are aligned with the wash head.

The wash pins are fixed to the wash head. During operation, the wash head assembly is lowered to insert the wash pins into the wells or raised to remove the wash pins from the wells. Lowering the wash head assembly allows the well contents to be aspirated or a bottom wash to be performed. Raising the wash head assembly allows the plate to be moved so another column (or row) can be washed or so the wells can be filled.

Twelve sets of dual wash pins for washing a 96-well microplate one row (12 wells) at a time.

Opsys MW™ User’s Guide 3

Description

Wash Head Positions

The vertical positions that the wash head can assume are described below. Each wash head position can be specified by the user to within 0.1 mm.

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Wash Head Position Description

Dispense Height

Well Top

Aspiration Height

Sweep Height

Note: Procedures for specifying wash head positions for various plate types are described on page 30.

Positions the aspiration pins slightly below the top of the well so that fluid can be dispensed into the well up to a certain level before it is aspirated.

Positions the aspiration pins so they are aligned with the top of the well.

Positions the aspiration pins at the bottom of the well so that the contents of the well can be completely aspirated.

Raises the aspiration pins slightly above the Aspiration Height (see above) so that the aspiration pins can be moved back and forth in the well while the fluid is being aspirated.

Bottom Wash Height

In protocols with a bottom wash, lowers the wash head during dispense so that the force of the dispensed fluid can wash the bottom of the wells.

4 Opsys MW™ User’s Guide

Description

Wash Fluid

A single wash fluid is used on the Opsys MW™ Microplate Washer. The wash fluid is contained in the Wash Container and is dispensed into the wells whenever a Dispense or Fill operation is specified in the wash protocol. In addition, wash fluid is dispensed into the purge tray during a Purge operation.

Dispensing of the fluid from the Wash Container is controlled by a pump attached to the back of the Opsys MW™ Microplate Washer and a dispense valve located near the wash head (Figure 1).

Waste

Fluid is removed from the wells during an Aspirate or Dispense operation and collected in the Waste Container. The Waste Container contains a level sensor to alert the operator when the container is full.

Removal of the fluid from wells is controlled by lowering the wash head so that the aspiration pins are immersed into the well fluid (the aspiration height). A vacuum is applied to the aspiration pins at all times during operation so that the contents of a well are aspirated whenever the wash head is lowered to the aspiration height.

Opsys MW™ User’s Guide 5

Description

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6 Opsys MW™ User’s Guide

Features

The Opsys MW™ system has a number of performance and convenience features. These are summarised below:

• Small footprint

• Up to 40 wash protocols can be programmed

• Up to ten plate types can be programmed

• Two wash head configurations (1 x 8 and 1 x 12)

• On-board self diagnostics

• SHOW function allows visual verification of wash head height

settings

• High-level alarm on Waste Container

Opsys MW™ User’s Guide 7

Features

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8 Opsys MW™ User’s Guide

Specifications

Dimensions and Weight

Length 49 cm (19.3 in) Width 38 cm (15.0 in) Height 19 cm (7.5 in) Weight 9.1 kg (20 lbs)

Operation

Dispense Range Dispense Precision Residual Aspirate Volumes

Washing Time Typical 5 cycle wash: 5 min 30 sec Power < 100 W Display 2 by 20 character back lit LCD Keypad 21 key tactile membrane Output Display, Printer, RS232

50 – 999 µL 5% CV (with 300 µL distilled water) < 1 µL for U and V bottom plates

< 5 µL for flat bottom plates

Environmental

Operating Range 15° C to 30° C

15% to 85% relative humidity (non-condensing) 2000m altitude

Computer Interface

Port Serial RS-232 port (Service Access) Baud Rate 19200. Character format Character Format 7 data bits, 1 stop bit, no parity

Power Requirements

Voltage Power Frequency

Main Unit 100 - 240 V 200 VA 50/60 Hz

Line Voltage Variation Line Frequency Variation

Opsys MW™ User’s Guide 9

10%

3 Hz

Specifications

Input Ranges

Assay Programming

Assay Name Up to 10 characters Password Up to 8 characters Move: Start Strip 1 - 8 for 1 x 8 heads and 1 - 12 for 1 x 12 heads (Increment: 1) Move: End Strip 1 - 8 for 1 x 8 heads and 1 - 12 for 1 x 12 heads (Increment: 1) Move: #Cycles 1 - 9 (Increment: 1) Aspirate: Cycles 1 - 9 (Increment: 1) Dispense Purge Fill Soak 1 - 999 (Increment: 1 second)

50 - 999 (Increment: 1 µL) 50 - 9999 (Increment: 1 µL) 50 - 300 (Increment: 1 µL)

Plate Setup

Plate Name Up to 10 characters Dispense Height 001 - 999 (Increment: 1) Top of Well Height 001 - 999 (Increment: 1) Aspirate Height 001 - 999 (Increment: 1) Sweep Height 001 - 999 (Increment: 1) Sweep Stroke 001 - 999 (Increment: 1) Bottom Wash: Dispense

Height

001 - 999 (Increment: 1)

Standards

The instrument is designed in accordance with CSA 1010.1-92, UL 3101-1, EN61010-1 and EN61326.

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Warning Labels

The Opsys MW™ or its components may contain certain labels that that either warn the user of a hazard or note an electrical connection. The labels that may be used on the Opsys MW™ system are described below.

Label Description

Alternating current is present.

(English) Caution symbol. Refer to the Routine Maintenance chapter.

(French) Attention (voir documents d’accompagnement).

(English) Caution, hot surface. (French) Attention, surface chaude.

Protective conductor terminal.

Earth (ground) terminal.

(English) Caution, risk of electric shock. (French) Attention, risque de choc électrique.

Opsys MW™ User’s Guide 11

Warning Labels

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12 Opsys MW™ User’s Guide

Unpacking

Chapter 2 Installation

Unpacking

Materials Provided

The main carton contains the instrument. The contents of this carton are listed below:

Article Quantity

Opsys MW™ System 1 Instrument Power Cable 1 Cleaning Wire, Aspirate 1 Cleaning Wire, Dispense 1 Instrument Configuration Report 1 User’s Guide 1 Declaration of Conformity 1 Wash Head, 1 x 8 or 1 x12 1 Plate Holder 1

A sub-carton contains the wash container and waste container assemblies. The contents of this carton are listed below:

Article Quantity

Wash Container Assembly 1 Waste Container Assembly 1

Opsys MW™ User’s Guide 13

Unpacking

To unpack the components:

1. Place the Opsys MW™ instrument in the position where it will be located for use.

2. Place the Wash Container, Waste Container and Foam/Aerosol Trap at the rear of the instrument.

3. Examine the packaging to be sure that the power cord and other materials have been removed. Please save packaging material for future use.

4. Verify that all of the materials listed above have been unpacked.

5. Inspect the components for damage. If damage is observed, contact your shipper or service representative immediately.

14 Opsys MW™ User’s Guide

Hardware Components

Hardware components of the Opsys MW™ instrument are shown in Figure 2 and described below.

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Wash Head Microtiter® Plate Plate Carrier Display Panel Keypad

Note: The Wash Container, Waste Container and Foam/Aerosol Trap are external to the instrument and are not shown in Figure 2.

Figure 2. The Opsys MW™ System

• Wash Head. The wash head contains sets (1 x 8 or 1 x 12) of dual wash pins. Each set of wash pins consists of a dispense pin and

an aspirate pin, closely spaced so that fluid can be aspirated from a well while fluid is being dispensed into the same well. The wash head moves back and forth to wash each row (or column, depending on the wash head that is used) of the plate by lowering the wash pins into the corresponding wells.

• Plate Carrier. The plate carrier holds the plate in a fixed position so that the wash pins can be precisely lowered to the correct height and position in the wells during the washing procedure.

• Display Panel. The display panel is the means by which the system communicates to the user. All messages, commands, and wash parameters entered by the user are displayed.

Opsys MW™ User’s Guide 15

Hardware Components

• Keypad. The keypad is used by the operator to select commands and enter wash parameters.

• RS232 Interface. The system includes an RS232 interface so that an external computer can be connected for technical service purposes.

• Wash Container. (Shown in Figure 1). The wash container contains the wash fluid that is used for Dispense, Fill and Purge operations.

• Waste Container. (Shown in Figure 1). The waste container contains the fluid that is aspirated from the wells. A level sensor is contained inside of the waste container.

16 Opsys MW™ User’s Guide

Hardware Components

Connectors at the rear of the Opsys MW™ system are shown in Figure 3. Connections for the power cord, dispense pump power, waste bottle level

sensor, waste bottle vacuum line and an external computer are made at the rear of the system.

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Important: Be sure that the vacuum exhaust is not obstructed.

Figure 3. Rear View of the Opsys MW™ System

Opsys MW™ User’s Guide 17

Positioning the Instrument

Determine the area where the system will be located. You will need a firm and level area that is approximately 51 cm (20 inches) wide, 62 cm (24 inches) deep, and 40 cm (16 inches) high for the Opsys MW™.

18 Opsys MW™ User’s Guide

Installing the Wash Head

The Wash Head must be located on the Wash Head Arm.

To install the Wash Head:

1. Position the Arm over the Purge Reservoir portion of the Plate Holder.

2. Place the Wash Head on the two Arm Pins, being careful to properly seat the Wash Head on the support pins.

3. Level the Wash Head in relation to the Plate Holder and tighten the two thumbscrews at the front of the Wash Head / Arm Assembly.

Opsys MW™ User’s Guide 19

Installing the Wash Container

The dispense tubing and dispense pump power cable must be connected.

To install the Wash Container:

1. Fill the Wash Container with the wash solution that is to be used.

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2. Insert the Pick-Up Filter into the Wash Container and secure the cap. Attach the Quick-Connector at the end of the pump tubing, to the mating connector in the cap.

3. Route the dispense tubing through the upper slot at the front corner of the instrument and through the routing clips at the front of the instrument.

4. Connect the end of the dispense tubing to the wash tubing connector on the wash head.

5. Thread the dispense tubing through the dispense valve. (Figure 1).

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6. Connect the dispense pump power cable to the dispense pump power connector at the rear of the instrument (Figure 3).

Note: The Wash Container contains up to 4 liters.

Note: The tubing must be inserted fully into the dispense valve opening to ensure proper operation.

20 Opsys MW™ User’s Guide

Installing the Waste Container

To install the Waste Container and Foam/Aerosol Trap:

1. Insert the Quick-Connect end of the vacuum tubing on the Foam/Aerosol Trap to the Quick-Connect vacuum connector at the rear of the instrument Figure 3).

2. Screw on the Waste Container cap.

3. Route the waste tubing through the lower slot at the front corner of the instrument and through the routing clips at the front of the instrument.

4. Connect the end of the waste tubing to the waste tubing connector on the wash head.

5. Connect the waste level sensor cable to the waste level sensor connector at the rear of the instrument (Figure 3).

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