Dako Hybridizer User Manual

4 (1)
Dako Hybridizer User Manual

PATHOLOGY

Hybridizer  Quick Start Guide

Hybridizer (120 V), Code S2450,

Hybridizer (240 V), Code S2451 &

Humidity Control Strips, Code S2452

Main Menu

 

Use arrow keys to move cursor up and down

Use arrow keys to scroll through character set

A-Z when naming program

Press“Enter” button to accept

 

Enter numeric

values for time and

 

 

temperature

 

Enter numeric

 

values in program

 

name

 

Press “STOP”

button to abort a

 

 

program in process

Press “Backspace” button to return to previous field or previous screen

Installation of the Hybridizer

nPlace the Hybridizer on a level surface suitable for laboratory instrumentation.

nThe Hybridizer has an intake fan located at the bottom and an outlet fan at the rear; assure no obstructions exist on intake and outlet.

Note: Avoid loose paper under the Hybridizer to prevent obstruction.

nPosition the Hybridizer away from direct sunlight and sources of heat or cold.

nVerify voltage requirements located on serial No. label, rear of instrument.

Connect Power and Turn on the Hybridizer

nPlug the Hybridizer into the appropriate outlet supplying the voltage and frequency indicated on the serial No. label.

nMove switch to “ON” position. The main power switch is located on the rear panel (I = On,

O = Off).

nInstrument will beep on start-up and the initialization screen will appear.

nThe Main Menu will be displayed.

Please use the system as intended. Improper use of the Hybridizer may cause damage to the system, inaccurate results, potentially nullify warranties, or cause personal injury.

Display Abbreviations

Humidity Control Strips

Slide Location

Select Program Type

S e l e c t P G M Ty p e

D e n a t & H y b

H y b O n l y

F i x e d Te m p

PGM:

Program

Denat & Hyb:

Denaturation & Hybridization

Denat Temp:

Denaturation Temperature

Denat Time:

Denaturation Time

Hyb Temp:

Hybridization Temperature

Hyb Time:

Hybridization Time

Hyb Only:

Hybridization Only

Fixed Temp:

Fixed Temperature

Heating to Fxd:

Heating to Fixed (-- °C)

 

 

nMinimize evaporation of sample from prepared slides.

nTo install strips, lift lid and insert strips into slot positions.

nSaturate strips with distilled water or equivalent.

nStrips should be replaced frequently as they will deteriorate over time and with use.

nAccommodates up to 12 slides per run.

nThe Hybridizer prompts to add slides.

nLift lid and load slides onto plate using slide locator for easy positioning.

nFrosted and/or label end of slides should be away from slide locator.

nMove slides across plate until they rest against slide locator.

Note: If slides are not resting against slide locator, closing of lid may break slides.

nThe Hybridizer is capable of storing 40 programs. Each program can be one of three program types:

-Denaturation and Hybridization

-Hybridization Only

-Fixed Temperature

nSelect program type and follow screen prompts to enter run times and set temperatures.

Abort Program in Process

A B O RT I N G ! !

A re Yo u S u re ?

N o

Ye s - M a i n M e n u

nPress “STOP” button to abort a program in process.

nUse arrows to move cursor to “Yes” line.

nPress “Enter” button to accept.

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