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PATHOLOGY
Hybridizer Quick Start Guide
Hybridizer (120 V), Code S2450,
Hybridizer (240 V), Code S2451 &
Humidity Control Strips, Code S2452
Main Menu
Enter numeric
values for time and
temperature
Enter numeric
values in program
name
Use arrow keys to
move cursor up and
down
Use arrow keys
to scroll through
character set
A-Z when
naming program
Press “Enter”
button to accept
Installation of the Hybridizer
n Place the Hybridizer on a level surface suitable
for laboratory instrumentation.
n The Hybridizer has an intake fan located at the
bottom and an outlet fan at the rear; assure no
obstructions exist on intake and outlet.
Note: Avoid loose paper under the Hybridizer to
prevent obstruction.
n Position the Hybridizer away from direct sunlight
and sources of heat or cold.
n Verify voltage requirements located on serial
No. label, rear of instrument.
Press “STOP”
button to abort a
program in process
Press “Backspace” button
to return to previous eld or
previous screen
Connect Power and Turn on the Hybridizer
n Plug the Hybridizer into the appropriate outlet
supplying the voltage and frequency indicated on
the serial No. label.
n Move switch to “ON” position. The main power
switch is located on the rear panel (I = On,
O = Off).
n Instrument will beep on start-up and the
initialization screen will appear.
n The Main Menu will be displayed.
Please use the system as intended. Improper use of the Hybridizer may cause damage to the system,
inaccurate results, potentially nullify warranties, or cause personal injury.
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Display Abbreviations
Humidity Control Strips
PGM: Program
Denat & Hyb: Denaturation & Hybridization
Denat Temp: Denaturation Temperature
Denat Time: Denaturation Time
Hyb Temp: Hybridization Temperature
Hyb Time: Hybridization Time
Hyb Only: Hybridization Only
Fixed Temp: Fixed Temperature
Heating to Fxd: Heating to Fixed (-- °C)
n Minimize evaporation of sample from prepared slides.
n To install strips, lift lid and insert strips into slot positions.
n Saturate strips with distilled water or equivalent.
n Strips should be replaced frequently as they will deteriorate
over time and with use.
Slide Location
Select Program Type
S e l ec t PG M Ty p e
D e n at & H yb
H y b O n l y
F i x ed Te m p
Abort Program in Process
n Accommodates up to 12 slides per run.
n The Hybridizer prompts to add slides.
n Lift lid and load slides onto plate using slide locator for
easy positioning.
n Frosted and/or label end of slides should be away from
slide locator.
n Move slides across plate until they rest against slide locator.
Note: If slides are not resting against slide locator, closing of lid may
break slides.
n The Hybridizer is capable of storing 40 programs. Each
program can be one of three program types:
- Denaturation and Hybridization
- Hybridization Only
- Fixed Temperature
n Select program type and follow screen prompts to enter
run times and set temperatures.
A B O RT IN G ! !
A r e Y o u S u r e ?
N o
Ye s - M a in M en u
n Press “STOP” button to abort a program in process.
n Use arrows to move cursor to “Yes” line.
n Press “Enter” button to accept.
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Create a Denaturation and Hybridization Program (Denat & Hyb)
1. Select Create a PGM 2. Select Program Type
Ru n a PG M
Ed it a P GM
Cr eat e a PG M
Pr ese nt Tem p: 37 °C
n Use the arrow keys to move
cursor to “Create a PGM”
n Press “Enter” button to accept
Se lec t P GM Type
De nat & Hyb
Hy b O nly
Fi xed Temp
n Cursor highlights “Denat &
Hyb” line
n Press “Enter” button to accept
3. Create a Name 4. Denaturation
Temp & Time
Cursor highlights the first
PG M 0 1 Nam e
En ter : Temp Ti me
De nat : °C :0 0
Hy b: °C 00:0 0
Note: All 10 character positions
must be filled.
6. Save a Program 7. Create a Hyb Only or Fixed Temp Program
De nat : 82 °C :0 5
Hy b: 45 °C 2 0:0 0
EN TER to Ac cep t
ST OP to Abo r t
n
name character position
n Use the arrow keys to scroll
through character set
Press “Enter” button to accept
n
characters one by one
n Press “Enter” button to accept
blank characters
n
Use keys 0-9 to enter numeric
characters
n Display will now show entered
program values
n Cursor highlights “Enter to
Accept” line
n Press “Enter” button to accept
program values
n Display indicates “Program
Saved”
n Cursor will advance to
“Denat Temp”
n Enter a two-digit temperature
value (50-99 °C)
Cursor will advance to
n
“Denat Time”
n Enter a two-digit time
value in minutes (0-30)
n Repeat procedure to create “Hyb Only” or “Fixed Temp” program
n Follow screen prompts to enter run times and/or set temperatures
n See Handbook for more detailed instructions
5. Hybridization
Temp & Time
n Cursor advances to “Hyb
Temp”
n Enter a two-digit temperature
value (30-70 °C). Enter “00”
for room temperature
hybridization
n Cursor advances to “Hyb Time”
n Enter a two-digit time value in
hours (0-99) followed by a
two-digit value in minutes
(0-59)
Edit a Program
1. Select Edit a PGM 2. Select a PGM
Ru n a PG M
Ed it a P GM
Cr eat e a PG M
Pr ese nt Tem p: 37 °C
3. Select Program Type 4. Enter New Values
Se lec t P GM Type
De nat & Hyb
Hy b O nly
Fi xed Temp
n Use the arrow keys to move
cursor to “Edit a PGM”
n Press “Enter” button to accept
n Cursor highlights existing
program type
n Move cursor with arrow keys
and press “Enter” to accept
program type
En ter PG M N o.
or sc rol l ( arrow s)
PG M 0 1 HE R2
PG M 0 4 Nam e H PV
En ter : Temp Ti me
De nat : 92 °C :0 5
Hy b: 37 °C 0 1:0 0
n Scroll through program
Nos. 1-40/program names
n Press “Enter” button to accept
n Use numeric keys
to enter new values for
temperatures and/or times
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Run a Denaturation and Hybridization Program (Denat & Hyb)
1. Select Run a PGM 2. Select a PGM
Ru n a PG M
Ed it a P GM
Cr eat e a PG M
Pr ese nt Tem p: 37 °C
3. Insert Humidity Control Strips and Add Slides 4. Heating to Denat Temp
PG M 0 1 HE R2
Ad d S lid es- Clo se Lid
St art
Ma in Men u
5. Denaturation 6. Cooling to Hyb Temp
PG M 0 1 HE R2
De nat in Pr oce ss
De nat : 8 2 ° C 02 :28
Pr ese nt Tem p: 82 °C
7. Hybridization 8. Process Completed
PG M 0 1 H ER2
Hy b i n P roc ess
Hy b: 45 °C 1 2 :4 8
Pr ese nt Tem p: 45 °C
n Cursor highlights “Run a
PGM” line
n Press “Enter” button to accept
n Scroll through program
Nos. 1-40/program names
n Press “Enter” button to accept
n Insert two Humidity Control
Strips into the inside slide lid
n Saturate strips with distilled
water or equivalent
n Add slides and close lid
n Cursor highlights “Start” line
n The Hybridizer will beep twice
once temperature reaches
denaturation set point
n Denaturation time will count
down from the set time
n Hybridization time will count
down from the set time
once temperature reaches
hybridization set point
PG M 0 1 HER 2
82 °C :0 5; 45 °C 20: 00
Ru n P GM
Ma in Men u
PG M 0 1 HER 2
** Hea tin g**
De nat : 8 2 ° C :0 5
Pr ese nt Tem p: 45 °C
Pl eas e Wai t
Co oli ng to Hyb 45 °C
Pr ese nt Tem p: 58 °C
PG M 0 1 HE R2
PR OCE SS COM PLE TE
Total Hy b T ime 20 :00
En d P GM/ Mai n M enu
n Display will conrm PGM No./
name and Denat & Hyb times
and temperatures
n Cursor highlights “Run PGM”
line
n Press “Enter” button to accept
n Press “Enter” button to run the
program
n Display indicates present
temperature of the slides
n The Hybridizer will
automatically cool to
hybridization set temperature
once denaturation is
completed
n The Hybridizer will beep
ve times upon program
completion
n Cursor highlights “End PGM/
Main Menu”
n Press “Enter” to end program
n See Handbook for further
details and for running a
“Hyb Only” or “Fixed Temp”
program
Corporate Headquarters
Denmark
Tel +45 44 85 95 00
Distributors in more
than 50 countries
www.dako.com
00187 25APR07
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