Dako Hybridizer User Manual

PATHOLOGY

Hybridizer  Quick Start Guide

Hybridizer (120 V), Code S2450,
Hybridizer (240 V), Code S2451 &
Humidity Control Strips, Code S2452

Main Menu



Enter numeric



values for time and
temperature

Enter numeric
values in program
name

Use arrow keys to
move cursor up and
down

Use arrow keys
to scroll through
character set
A-Z when
naming program





Press “Enter”
button to accept

Installation of the Hybridizer

n Place the Hybridizer on a level surface suitable

for laboratory instrumentation.

n The Hybridizer has an intake fan located at the

bottom and an outlet fan at the rear; assure no
obstructions exist on intake and outlet.
Note: Avoid loose paper under the Hybridizer to
prevent obstruction.

n Position the Hybridizer away from direct sunlight

and sources of heat or cold.

n Verify voltage requirements located on serial

No. label, rear of instrument.

Press “STOP”



button to abort a
program in process



Press “Backspace” button
to return to previous eld or
previous screen

Connect Power and Turn on the Hybridizer

n Plug the Hybridizer into the appropriate outlet

supplying the voltage and frequency indicated on
the serial No. label.

n Move switch to “ON” position. The main power

switch is located on the rear panel (I = On,
O = Off).

n Instrument will beep on start-up and the

initialization screen will appear.

n The Main Menu will be displayed.

Please use the system as intended. Improper use of the Hybridizer may cause damage to the system,
inaccurate results, potentially nullify warranties, or cause personal injury.

Display Abbreviations

Humidity Control Strips

PGM: Program

Denat & Hyb: Denaturation & Hybridization

Denat Temp: Denaturation Temperature

Denat Time: Denaturation Time

Hyb Temp: Hybridization Temperature

Hyb Time: Hybridization Time

Hyb Only: Hybridization Only

Fixed Temp: Fixed Temperature

Heating to Fxd: Heating to Fixed (-- °C)

n Minimize evaporation of sample from prepared slides.
n To install strips, lift lid and insert strips into slot positions.
n Saturate strips with distilled water or equivalent.
n Strips should be replaced frequently as they will deteriorate

over time and with use.

Slide Location

Select Program Type

S e l ec t   PG M  Ty p e

D e n at   &  H yb

H y b  O n l y

F i x ed   Te m p

Abort Program in Process

n Accommodates up to 12 slides per run.
n The Hybridizer prompts to add slides.
n Lift lid and load slides onto plate using slide locator for

easy positioning.

n Frosted and/or label end of slides should be away from

slide locator.

n Move slides across plate until they rest against slide locator.

Note: If slides are not resting against slide locator, closing of lid may
break slides.

n The Hybridizer is capable of storing 40 programs. Each

program can be one of three program types:

- Denaturation and Hybridization

- Hybridization Only

- Fixed Temperature

n Select program type and follow screen prompts to enter

run times and set temperatures.

A B O RT IN G ! !

A r e  Y o u  S u r e ?

N o

Ye s  -  M a in   M en u

n Press “STOP” button to abort a program in process.
n Use arrows to move cursor to “Yes” line.
n Press “Enter” button to accept.