Dako Hybridizer User Manual
Hybridizer | Handbook
Dako Hybridizer | Handbook 1
Hybridizer | Handbook
Document Number 0003061
Revision C
July 2007
For In Vitro Diagnostic Use
Dako Colorado, Inc.
4850 Innovation Drive
Ft. Collins, CO 80525 USA
970-226-2200
Dako Hybridizer (120 V) - Code No. S2450
Dako Hybridizer (240 V) - Code No. S2451
Dako Hybridizer Control Strips - Code No. S2452
2 Dako Hybridizer | Handbook
©
Copyright
This document may not be copied in whole or in part or reproduced in any other media without the
express written permission of Dako. Please note that under copyright law, copying includes translation
into another language.
2007 Dako Colorado, Inc. All rights reserved
Contact Dako
Manufacturer
Corporate Headquarters
Dako Colorado, Inc.
4850 Innovation Drive
Fort Collins, CO 80528 USA
www.dako.com
Dako Denmark A/S
Produktionsvej 42
DK-2600 Glostrup Denmark
www.dako.com
User Resources
For the latest information on Dako products and services, please visit the Dako website at:
http://www.dako.com
Customer Support
Contact your local Dako representative for customer support. Visit the Dako website for the most current
support information.
Scope
This document contains basic information on the use and operation of the Hybridizer and assumes you
have received basic training on the instrument. Please contact your Dako representative for information
not provided in this manual.
Dako Hybridizer | Handbook 3
Table of Contents | Handbook
Section 1 | Unpacking and Installation................................................................... 8
Inspect Packaging...........................................................................................................................8
Verify Contents................................................................................................................................ 8
Installation of the Hybridizer............................................................................................................ 8
Connect Power................................................................................................................................ 8
Section 2 | System Overview................................................................................. 10
Principle and Intended Use........................................................................................................... 10
Symbols and Definitions ...............................................................................................................10
Keyboard and Main Menu Screen ................................................................................................10
Symbols and Definitions (cont.) .................................................................................................... 11
Audible Indicators..........................................................................................................................12
Audible Error Indicators ................................................................................................................12
Error Messages.............................................................................................................................12
Section 3 | Programming ....................................................................................... 16
Opening and Closing the Lid......................................................................................................... 16
Turn Unit On..................................................................................................................................16
Overview ....................................................................................................................................... 16
Create a Denaturation and Hybridization Program (Denat & Hyb)............................................... 17
Create a Hybridization Only Program (Hyb Only)......................................................................... 18
Create a Fixed Temperature Program (Fixed Temp) ...................................................................19
Section 4 | Operating Instructions........................................................................ 20
Opening and Closing the Lid......................................................................................................... 20
Turn Unit On..................................................................................................................................20
Run a Denat & Hyb Program ........................................................................................................ 20
Run a Hyb Only Program.............................................................................................................. 22
Run a Fixed Temp Program.......................................................................................................... 24
Abort Program in Process............................................................................................................. 25
Recommended for Best Results ................................................................................................... 26
Slide Location................................................................................................................................ 26
Humidity Control Strips .................................................................................................................26
Predefined Limits ..........................................................................................................................27
4 Dako Hybridizer | Handbook
Section 5 | Edit a Program..................................................................................... 28
Opening and Closing the Lid......................................................................................................... 28
Turn Unit On..................................................................................................................................28
Edit a Program .............................................................................................................................. 28
Section 6 | Maintenance......................................................................................... 30
Overview ....................................................................................................................................... 30
Cleaning ........................................................................................................................................ 30
Service .......................................................................................................................................... 30
Troubleshooting ............................................................................................................................31
Appendix A | Specifications .................................................................................. 32
Dako Warranty .............................................................................................................................. 32
Limitation of Liability...................................................................................................................... 32
Dako Hybridizer | Handbook 5
How to use this handbook
This handbook along with information contained on product labels should provide all the information
needed to operate and maintain the Hybridizer.
Notes appear in italics to highlight information. When the information requires special attention, a caution
symbol appears next to the italicized text:
Please pay close attention to the instructions that accompany the notes and symbols as well as the
standard laboratory practices outlined by your facility and local regulatory agencies. The table below lists
all the CAUTIONS / WARNINGS for the Hybridizer.
WARNING - North American Installation: Line cord should be plugged into a
properly grounded AC outlet.
WARNING - Outside of North America: Inspect that the supplied Line Cord has
local electrical compatibility.
EU Installation:
Use power cord with an IEC320/CEE22 female connector and male connector
suitable for the power outlet to be used. Cord must meet
CAUTION – Unplug the Hybridizer from the wall outlet before performing
maintenance.
WARNING – Do not expose Hybridizer to strong or concentrated acids, bases,
esters, aromatic or halogenated hydrocarbons, ketones or strong oxidizing agents.
standards.
BIOHAZARD - Universal Precautions should be followed on all specimens,
regardless of whether a specimen is known to contain an infectious agent.
CAUTION –Risk of electric shock: The instrument contains no user serviceable
parts. Removal of housing will expose potentially lethal voltage. Refer service to
qualified Dako service personal.
CAUTION – Hot Surface: The interior surface of the instrument may be HOT, use
caution to avoid potential burn.
Please use the system as intended. Improper use of the Hybridizer may cause damage to the system,
inaccurate results, or potentially nullify warranties.
6 Dako Hybridizer | Handbook
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Dako Hybridizer | Handbook 7
Section 1 | Unpacking and Installation
Inspect Packaging
The Hybridizer and its accessories are delivered in one carton. If the instrument or accessories have
suffered any damage in transport, please inform your carrier immediately.
Note: Save shipping carton and components to simplify return should service be required.
Verify Contents
The package contains:
One Hybridizer, code No. S 2450 (120V) or S 2451 (240V)
One Line Cord
One Handbook 30195
One CD (Handbook and Humidity Control Strips package insert) 30196
One Package Humidity Control Strips, code No. S 2452
Installation of the Hybridizer
1. Place the Hybridizer on a level surface suitable for laboratory instrumentation.
2. Hybridizer has an intake fan located on bottom and fan outlet in rear; assure no obstructions exist on
intake or outlet.
3. Position the Hybridizer away from direct sunlight and sources of heat or cold.
4. Verify voltage requirements located on serial number label, rear of instrument.
Connect Power
Plug the instrument into the appropriate outlet supplying the voltage and frequency indicated on the serial
number label. Main power switch is located on the rear of the instrument, next to the line cord power
entry module.
WARNING - North American Installation. Line cord should be plugged into a
properly grounded AC outlet.
WARNING - Outside of North America. Inspect that the supplied Line Cord got
local electrical compatibility.
EU Installation - Use power cord with an IEC320/CEE22 female connector and
male connector suitable for the power outlet to be used. Cord must meet
standards.
8 Dako Hybridizer | Handbook
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Dako Hybridizer | Handbook 9
Section 2 | System Overview
Principle and Intended Use
For in vitro diagnostic use to co-denature and hybridize probes to targets in
IVD
The Hybridizer is a microprocessor controlled small bench top hot plate with lid. The Hybridizer allows
storage of 40 programs, of 3 operating options; Denaturing and Hybridization, Hybridization Only or Fixed
Temperature. Capacity for 12 slides and maximum temperature of 99°C. The instrument is UL / CUL
listed and CE marked.
Symbols and Definitions
specimens on glass microscope slides.
8
Up
Down
Enter Accept or Enter
Backspace
Stop
0-9
Keyboard and Main Menu Screen
Move cursor up; Enter character A-Z for
program name
Move cursor down; Enter character A-Z for
program name
Move cursor back to previous screen
End a program in process
Enter numeric values for time and
temperature or for program name
10 Dako Hybridizer | Handbook
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