NucleoView® NC-200™
Software User’s Guide
P/N 991-0204
Revision 1.1
NucleoView®NC-200™
P/N 991-0204 (English)
Revision 1.1
September, 2012
ChemoMetec A/S
Gydevang 43, DK-3450 Allerod, Denmark
Telephone: (+45) 48 13 10 20,
Fax: (+45) 48 13 10 21
Internet: www.chemometec.com,
E-mail: sales@chemometec.com
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Caution!
This software must be operated as described in this User’s Guide and documents referred to herein. Please read the entire guide and referred documents before attempting to use this software.
Contacting support
Technical information including product literature, answers to questions regarding the operation of the NucleoView NC-200 not covered in this document and referred documents is available through the following:
For e-mail support, send questions to NucleoCounter NC-200 Technical Support on the address support@chemometec.com
Check out the FAQ section under support at www.chemometec.com
To speak with a Technical Support Specialist, call (+45) 48 13 10 20.
Please note the NucleoCounter NC-200 serial number and have it available when contacting ChemoMetec A/S for support. The NucleoCounter NC-200 serial number is found on the label affixed to the bottom of the instrument. The version number of the NucleoView NC-200 software shall also be noted, this can be found on the Help – About menu item in the NucleoView NC-200 software.
Sales and ordering information
For sales assistance with NucleoCounter NC-200 or the NucleoView NC-200 software, to place an order for a NucleoCounter NC-200 or consumables, call (+45) 48 13 10 20, fax (+45) 48 13 10 21, or send e-mail to sales@chemometec.com
Disclaimer Notices
The material in this document and referred documents is for information only and is subject to change without notice. While reasonable efforts have been made in preparation of these documents to assure their accuracy, ChemoMetec A/S assumes no liability resulting from errors or omissions in these documents, or from the use of the information contained herein.
ChemoMetec A/S reserves the right to make changes in the product design without reservation and without notification to its users.
Copyright Notices
Copyright © ChemoMetec A/S 2012. All rights reserved. No part of this publication and referred documents may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, recording or otherwise, without the prior written consent of ChemoMetec A/S, Gydevang 43, DK-3450 Allerod, Denmark.
ChemoMetec and NucleoCounter are registered trademarks owned by ChemoMetec A/S. NucleoCounter, NucleoCassettes, Via1-Cassette and NucleoView are trademarks of ChemoMetec A/S.
All other trademarks are the property of their respective owners.
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NucleoView® NC-200™....................................................................................................................................................... |
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User’s Guide ....................................................................................................................................................................... |
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Quick Guide................................................................................................................................................................ |
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Installation Guide ....................................................................................................................................................... |
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Installation Overview ..................................................................................................................................................... |
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Minimum Computer Requirements and Preparations before Installation .................................................................... |
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Installation of NucleoView® NC-200™ Software and NucleoCounter® NC-200™ Instrument ....................................... |
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Installation of Licenses and Protocols .......................................................................................................................... |
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Software Upgrade ........................................................................................................................................................ |
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Installation of a new NucleoCounter® NC-200™ Instrument ....................................................................................... |
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Uninstallation of NucleoView® NC-200™ Software...................................................................................................... |
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Main Window............................................................................................................................................................ |
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Menu Line..................................................................................................................................................................... |
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Selecting a Protocol in the Main Window.................................................................................................................. |
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Analysis Results ......................................................................................................................................................... |
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Browse Analysis Results ............................................................................................................................................ |
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File Browser............................................................................................................................................................... |
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Report Generator ...................................................................................................................................................... |
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Image Overlay on the User Interface ......................................................................................................................... |
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Next Run.................................................................................................................................................................... |
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Protocols ................................................................................................................................................................... |
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Protocol Adaptation Wizard ...................................................................................................................................... |
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Plot Manager............................................................................................................................................................. |
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Layout Editing............................................................................................................................................................... |
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Plot Statistics ................................................................................................................................................................ |
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Row Window in Plot Manager...................................................................................................................................... |
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Histogram Plot in Plot Manager ................................................................................................................................... |
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Large Histogram Plot in Plot Manager ......................................................................................................................... |
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Scatter Plot in Plot Manager ........................................................................................................................................ |
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Large Scatter Plot in Plot Manager............................................................................................................................... |
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Plot Manager Gate Configuration ................................................................................................................................ |
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Options...................................................................................................................................................................... |
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21 CFR Part 11 ........................................................................................................................................................... |
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Requirements on the Microsoft Windows Computer System ..................................................................................... |
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NucleoView® NC-200™................................................................................................................................................. |
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Event Log ...................................................................................................................................................................... |
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License, Protocol and Documentation Installation..................................................................................................... |
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Introduction.................................................................................................................................................................. |
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License, Protocol and Documentation Installation ...................................................................................................... |
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Maintenance and Backup .......................................................................................................................................... |
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NucleoView® NC-200™ Software Maintenance ........................................................................................................... |
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NucleoCounter® NC-200™ Instrument Maintenance................................................................................................... |
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How To ...................................................................................................................................................................... |
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How to Get Context Sensitive Help .............................................................................................................................. |
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How to Verify Correct Operation of the NucleoView® NC-200™ Instrument after Transportation............................. |
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Keyboard Shortcuts ................................................................................................................................................... |
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Trouble Shooting ....................................................................................................................................................... |
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Quick Guide
1.Install the NucleoView® NC-200™ software and the NucleoCounter® NC-200™ instrument as described in the documentation on the USB stick containing the NucleoView® NC-200™ software.
2.Start the NucleoView® NC-200™ software by double-clicking NucleoView® NC-200™ icon on the desktop.
3.Wait while the NucleoCounter® NC-200™ instrument initializes (you will hear motors working). When the LED on the instrument turns green the instrument is ready.
4.Click the 
button (below the F3 button) and select the protocol you want to use.
5.Click the 
button to see the application note for the selected protocol.
Check that you have all accessories needed for this protocol (cassettes, reagents ...).
6.Fill the cassette with the sample and place it in the cassette fixture.
7.Optional: Write a descriptive text in the Sample ID field and enter a user name in the Operator field.
8.Press the Run button.
A few moments later the results of the analysis will be displayed.
9.Repeat steps 6 to 8 to perform more analyses of the same kind or repeat from step 4 to start a new analysis.
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Installation Guide
Installation Overview
Important: You must be logged on as system administrator to install the NucleoView® NC-200™ system components.
In a first time installation, you will typically install both the NucleoView® NC-200™ software and one or more sets of NucleoCounter® NC-200™ instrument specific configuration data. This involves installation and activation of various USB drivers on one or more USB ports.
To uninstall the software just follow the un-installation procedure.
Minimum Computer Requirements and Preparations before Installation
Following PC requirements must be fulfilled before you can perform the installation:
1.Operating System: Windows 7 or Windows XP with service pack 3. The Windows operating system must not be virtualized.
OBS: Windows Vista is not supported!
2.You must be able to log on with administrator rights.
3.At least 2 GB RAM and 10GB free disc space is recommended for most applications.
4.At least one USB 2.0 port must be available.
5.Screen minimum 1024 x 768 pixels.
To perform the installation you will also need the NucleoCounter® NC-200™ instrument and the NC-200 Package which comprises power supply, cables and the USB stick containing the software. IMPORTANT: Make sure the serial number printed on the instrument matches the serial number on the USB stick.
Installation of NucleoView® NC-200™ Software and NucleoCounter® NC-200™ Instrument
This section describes software and instrument installation on Windows 7. For other versions of Windows there will be minor variations, but as a general rule, simply accept all the default settings as they are presented.
Installation of software and instrument typically takes 5-8 minutes.
1.See the previous section about minimum computer specifications and preparations.
2.Ensure that the NucleoCounter® NC-200™ instrument is NOT connected to the PC.
3.IMPORTANT: Log on with administrator rights for this installation session.
4.Insert the USB stick holding the software (if this has not already been done).
5.After a few seconds Windows will detect the USB stick and present an AutoPlay window if AutoPlay is enabled.
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Select the Open folder to view files option.
6.The root directory contents on the USB stick will be shown. Double click on the Install NucleoView® NC-200™ X.X.X.X file (the version number e.g. 1.0.22.0 will vary depending on your installation) to launch the installation program.
NB: It is the .exe file you should double click, not the .bin file. The .exe file also has the installation icon associated in contrast to the icon for the .bin file.
7.If a window with the text ‘Do you want to allow the following program from an unknown publisher to make changes to this computer?’ appears, click Yes.
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8.After a few seconds the ‘Welcome to the NucleoView® NC-200™ Install Wizard’ window will appear. Click Next.
9.On the Prerequisites window you must check the check box to confirm that you have disconnected any NucleoCounter instrument from the PC.
Click Next.
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10.On the ‘Ready to install NucleoView® NC-200™’ window you will be given options to install or upgrade the NucleoView® NC-200™ software itself, to install configuration data for specific instrument(s) and to browse for instrument configuration data which are stored elsewhere. Check the options that suites you and click Next.
11. On the License Agreement window read the license agreement, select ‘I accept ...!’ and click Next.
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12.On the ‘Select Additional Tasks’ window check the desired options and click Next.
Checking the ‘Associate ChemoMetec Files with the NucleoView application’ will register image files *.cm and protocol files *.cmsx with the NucleoView application for file double click methods.
13. On the ‘Ready to install’ window click Next.
The ‘Installing’ window will appear and show progress of extraction and copying of files. During the installation some windows may seem to hang, however redistributable files from
Microsoft or the .Net 4.0 framework are being installed. Please wait for these files to install and do not close the window. This process may take several minutes.
You may also see a command prompt window pop up for a short while, reporting about installation of a driver.
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14.On the ‘Window Security’ window click Install. To avoid this window during future software upgrades check the box Always trust software from “IDS Imaging Development Systems GmbH”.
15.On the ‘Window Security’ window click Install. To avoid this window during future software upgrades check the box Always trust software from “MATRIX VISION GmbH”.
16.On the ‘Completing the NucleoView® NC-200™ Install Wizard’ window you may optionally deselect the check box if you don't want to install any instrument.
Click ‘Finished’.
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17.The Install program will now have placed two new icons on your desktop. One is the NucleoCounter® NC-200™ instrument icon used to launch the NucleoView® NC-200™ software, the other is a shortcut to the data folder where NucleoView® NC-200™ stores application data.
At this point the NucleoView® NC-200™ software has successfully been installed.
The NucleoView® NC-200™ program will now automatically be started and it will guide you through the instrument installation procedure, if the check mark for “Install NucleoCounter® NC-200™ instrument after Install has finished” was checked.
NB: Depending on file versions and previous install history, a reboot of the computer may be requested before the NucleoView® NC-200™ program is launched. If a reboot is needed, it is essential that you log on using the same administrator user as used during the above installation.
1.NucleoView® NC-200™ is now launched in installation guided mode. Click ‘OK’ to continue with installation of the NucleoCounter® NC-200™ Instrument.
2.Follow the on-screen instructions (using the supplied power supply and USB cable). Note that the instrument icon in the upper left corner of the application bar is red while no instrument is attached.
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3.You will now see a series of pop-up messages from the Windows operating system informing about driver installations. Among those are:
-A USB hub inside the NucleoCounter® NC-200™ instrument
-FTDI device driver installed
-The camera
Due to the intentional and very fast reconfiguration of the camera, you may see an error message from Windows, which can just be ignored. Click Close on any of these pop up messages if needed.
When the NucleoView® NC-200™ software detects and has initialized the instrument, the previous red icon changes to true life colors indicating the instrument has been successfully installed.
4.NucleoView® NC-200™ will display a message box stating successful installation of the instrument, and recommend that you continue installation of this instrument on the remaining USB ports on the PC. Click ‘Yes’ to do so.
5.You will then be prompted to unplug the USB cable from the USB port on the PC. Do this and click
‘OK’.
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6.When NucleoView detects that the instrument has been disconnected, the icon will again turn red, and you will be prompted to connect the instrument to another USB port on the PC.
7.Repeat the procedure described to install the instrument on the remaining USB ports on the PC.
8.If you are installing more than one instrument on the same PC, you must repeat the USB installation for all instruments on all USB ports, but note that only one instrument must be attached at any time.
Installation of Licenses and Protocols
Follow the procedure described in the Licenses section to install licenses and protocols. This is not needed during a normal installation procedure, as licenses and protocols are installed automatically.
Software Upgrade
New software releases are typically distributed via our web page or on USB sticks and are installed as described below. Collected data (images), settings and installed licenses will be preserved.
1.IMPORTANT: Log on with administrator rights for this upgrade session.
2.Disconnect any NucleoCounter® NC-200™ instrument connected to the PC.
3.Insert the USB stick holding the software upgrade or download and unpack the software upgrade.
4.Continue as described in the previous section installation of software and the NucleoView® NC200™ Install Wizard will now guide you through the remaining part of the upgrade.
Installation of a new NucleoCounter® NC-200™ Instrument
To install one or more new NucleoCounter® NC-200™ instruments, use the USB stick holding the appropriate NucleoView® NC-200™ software release and follow the procedure in the previous section describing installation of software. The NucleoView® NC-200™ Install Wizard will guide you through the remaining part of the upgrade. While following this procedure, you will be given the option to specify which NucleoCounter® NC-200™ devices you want installed (identified by their serial numbers). Be sure to check the boxes which specify the new serial number(s) you want installed.
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You will also be given the option to browse for and select instrument data stored elsewhere.
IMPORTANT: You may install multiple instruments on the same PC, but be aware that NucleoView® NC200™ is only able to operate with one instrument at any time. You must never try to operate with more than one instrument as behavior may not be correct.
Uninstallation of NucleoView® NC-200™ Software
Either uninstall via Add/Remove Programs in the Control Panel, or run the un-installer via the ChemoMetec ->NucleoView® NC-200™->Uninstall NucleoView® NC-200™ in the Start Menu. This may e.g. be required if you wish to downgrade to an earlier version.
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