Bio-Rad Q Media User Manual
................................................................................................................................
Nuvia™ S
Ion Exchange
Instruction
Catalog
and Q High-Capacity
Media
Manual
numbers
156-0311 156-0411
156-0313 156-0413
156-0315 156-0415
156-0317 156-0417
Please read these instructions before you use Nuvia S and
Q High-capacity ion exchange media. If you have any
questions or comments regarding these instructions,
please contact your Bio-Rad Laboratories representative.
Table of Contents
Section
Section
Section
Section
Section
Section
Section
Section
Section
Section
Section
.
1:
Introduction 1
2 :
Technical Description 1
3 :
Preparation 2
4 :
Column
5 :
Column Packing Evaluation 3
6 :
Operation
7 :
Regeneration
8 :
Cleaning-in-Place
9 :
Storage 8
10:
Regulatory Support
11: Ordering
Packing
and
Maintenance
and
Sanitation 7
(CIP) and
Information 8
5
Sanitation 8
2
8
Section
Nuvia™ S and Q
Introduction
1:
high-capacity
ion
exchange
binding capacity chromatography media
purification process from capture to
properties,
biomolecule
process scale.
nucleic acids,
high productivity, and
purification
Nuvia
at both
media are
viruses, plasmids,
offer high binding capacity at fast
backpressure. The unique properties
highly effective
If you
have questions
media for biomolecule purification.
about
Nuvia media,
Rad process chromatography
chromatography
Technical
Support department for
final polish. Their
exceptional selectivity
laboratory scale
designed
and other
linear
flow
of
sales representative
1-510-741-6563.
:
Section
Technical
2
Description
media are new
designed
for any stage in the
robust
meet the needs for
and
for the
biopharmaceutical
separation
macromolecules. Nuvia
rates
while
Nuvia
media position them as
contact
maintaining
either
your local Bio-
or the Bio-Rad
further assistance
ultra high
physical
of
proteins,
media
low
at
Table 1.
Nuvia S Nuvia Q
Type of ion exchanger Strong cation Strong anion
Functional
Total ionic capacity 90–150 µeq/ml 100-170 µeq/ml
Dynamic binding capacity* 300
Shipping counterion Na
Median
Recommended linear
Chemical
1.0 N
0.1 N
0.01 N
Gel bed
pH stability** 2–14 short term 2–14 short term
Shipping solution 20%
Regeneration 1–2 M NaCl 1–2 M NaCl
Sanitation 0.5–1.0 N NaOH 0.5–1.0 N NaOH
Storage conditions 20%
* 10%
NaCl,
**Data
NOTE: Recommended elution flow rates are <150 cm/hr to minimize viscosity induced
backpressure.
Characteristics
group –SO
particle
size 85 ± 15 µm 85 ± 15 µm
stability
NaOH
NaOH
NaOH
compression
breakthrough
pH 5.0 for Nuvia S and 5 mg/ml BSA in 20 mM Tris-HCI pH 8.5 for Nuvia Q.
derived
flow rate range 50–600 cm/hr 50–600 cm/hr
(20°C) up to 1 week up to 1 week
(20°C)** up to 5
(20°C)** — up to 5 yr
ratio 1.15–1.18
capacity
under
accelerated
of Nuvia™ media.
-
3
cm/hr
+ Cl-
volume/packed
4–13 long term 4–12 long term
ethanol
ethanol
determined
with 4.5 mg/ml
conditions at 60°C.
–N(CH3)
>110 mg/ml
yr —
(settled
bed 1.10–1.15
bed volume)
+ 0.1 M NaCl 20%
or 0.1 N NaOH 20%
human
300 cm/hr >170 mg/ml
IgG in 40 mM
+
3
ethanol
ethanol
NaOAc
+ 1 M NaCl
or 0.01 N NaOH
+ 30 mM
1
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