A&D FX-300i-PT User Manual
Pipette Accuracy Tester
PT-Series
AD-4212B-PT
A
D-4212A-PT
FX-300i-PT
INSTRUCTION MANUAL
1WMPD4001794
© 2009 A&D Company, Limited. All rights reserved.
No part of this publication may be reproduced, transmitted, transcribed, or translated
into any language in any form by any means without the written permission of A&D
Company, Limited.
The contents of this manual and the specifications of the instrument covered by this
manual are subject to change for improvement without notice.
Microsoft, Windows, Excel and Word are either registered trademarks or trademarks of
Microsoft Corporation in the United States and/or other countries.
CONTENTS
1. INTRODUCTION .............................................................................................................................2
1-1 Unpacking the Tester.......................................................................................................................... 2
1-2 Description of Each Component......................................................................................................... 4
1-3 WinCT-Pipette, Software for Pipette Accuracy Testing....................................................................... 4
1-4 Function of the Evaporation Trap ....................................................................................................... 5
2. ASSEMBLING THE TESTER..........................................................................................................6
2-1 AD-4212B-PT / AD-4212A-PT ............................................................................................................ 6
2-2 FX-300i-PT ......................................................................................................................................... 8
3. PRECAUTIONS BEFORE USE ....................................................................................................10
4. ANTI-STATIC TREATMENT..........................................................................................................11
5. INSTALLING WinCT-Pipette ........................................................................................................11
6. CONNECTING A PC TO THE BALANCE.....................................................................................12
6-1 Connecting a PC .............................................................................................................................. 12
6-2 Checking COM Port Numbers.......................................................................................................... 13
7. CALIBRATING THE BALANCE ...................................................................................................14
7-1 AD-4212B-PT / AD-4212A-PT .......................................................................................................... 14
7-2 FX-300i-PT ....................................................................................................................................... 15
8. PREPARATION BEFORE USE.....................................................................................................16
8-1 Filling the Evaporation Trap with Water............................................................................................ 16
8-2 Preparing the Test Liquid .................................................................................................................. 16
8-3 Using the Thermometer.................................................................................................................... 17
9. MEASUREMENT ..........................................................................................................................21
9-1 Measuring Procedure ....................................................................................................................... 21
9-2 Judging the Results .......................................................................................................................... 21
9-3 Example Causes of Measurement Errors ........................................................................................22
10. AD-4212B-PT / AD-4212A-PT ....................................................................................................24
10-1 Factory Settings.............................................................................................................................. 24
10-2 Display Lock Function .................................................................................................................... 25
11. TROUBLESHOOTING ................................................................................................................26
12. SPECIFICATIONS .......................................................................................................................27
13. APPENDIX ..................................................................................................................................28
1
1. INTRODUCTION
The PT series Pipette Accuracy Tester performs an accuracy test of the volume of a pipette in
accordance with ISO 8655.
This manual describes how the PT series Pipette Accuracy Tester works and how to get the most out
of it in terms of performance. It includes how to assemble the tester, how to measure and how to use
the software, WinCT-Pipette, for pipette accuracy testing.
Read this manual thoroughly before using the tester and keep it at hand for future reference.
For detailed information on handling the balance, refer to the instruction manual for the balance used.
NOTE ISO 8655 is the international standard that includes pipette calibration and specifies a test
method for accuracy of the pipette volume. For detailed information, visit our web site.
1-1 Unpacking the Tester
NOTE The pipette accuracy tester is contained in a carrying case. The case is only for
carrying the tester and is not solid enough to endure transportation. So, do not
transport the tester using the carrying case. When transporting the tester, be sure to
use the original packing material.
The illustrations on the next page show what the PT series consists of.
When handling the tester, be careful not to drop it, even if it is placed in the carrying case.
Note that the USB converter is shipped in a separate package, but it can be placed in the carrying
case as shown below.
A shoulder belt and a key are provided with the carrying case. Use the shoulder belt to carry the
case.
NOTE Please confirm that the AC adapter type is correct for your local voltage and power
receptacle type.
2
AD-4212B-PT / AD-4212A-PT
The connection cable provided as an accessory is one meter long.
Display
Stan d
USB converter
RS-232C cable
(25P-9P)
Connection
cable (1 m)
Weighing unit
Carrying
case
Sample cup holder
Sample cup holder
(30 mL)
(5 mL)
Cap A
Cap B
Evaporation trap
Evaporation trap
base
Test liquid cup
Capacity:5 mL (2 pcs)
Capacity:30 mL (2 pcs)
Sample cup
AC adapter
Angle
adjustment
knob
Shoulder
belt
WinCT-Pipette
(CD-ROM)
Thermometer
Thermometer
clamp
Tweezers
Key
Weighing pan unit
Instruction manual
for the balance
Instruction manual
for the tester
Calibration
weight
(50 g)
Breeze break
FX-300i-PT
Evaporation trap
Evaporation trap
Balance
Instruction manual
for the tester
Instruction manual
for the balance
WinCT-Pipette
(CD-ROM)
Cap A
Cap B
base
Fitting boss
Capacity:5 mL (2 pcs)
Capacity:30 mL (2 pcs)
Sample
cup
USB converter
Carrying
case
Sample cup
holder (30 mL)
Sample cup
holder (5 mL)
Test liquid cup
RS-232C cable
(9P-9P)
AC adapter
Thermometer
Thermometer
clamp
Tweezers
Weighing pan unit
Small breeze break
Shoulder
belt
Key
Calibration
weight
(100 g)
3
1-2 Description of Each Component
z Evaporation trap
A chamber to prevent the evaporation of the dispensed test liquid.
(Refer to “1-4 Function of the Evaporation Trap”.)
z Sample cup (Two types: 5 mL and 30 mL)
A cup to contain the test liquid dispensed from a pipette.
z Test liquid cup
A container to keep a test liquid such as distilled water.
z Thermometer (with a clamp)
Measures the temperature of the test liquid.
z RS-232C cable and USB converter
Connects to a PC (personal computer) to transmit the test values to the PC.
z WinCT-Pipette
Software for pipette accuracy testing to convert the test liquid mass
into a volumetric value.
z Calibration weight (with a pair of tweezers to handle the weight)
A weight to calibrate the balance.
1-3 WinCT-Pipette, Software for Pipette Accuracy Testing
Features
The mass value of the distilled water dispensed from a pipette is transmitted from the balance to a
PC. Using the temperature of the test liquid and the barometric pressure that are previously entered,
the PC calculates the conversion factor (Z factor) from a mass value to a volumetric value and
converts the mass value into a volumetric value automatically, using the Z factor.
The measurement results are compared with the specifications previously entered, to perform
judgment whether the pipette will pass or fail.
Besides the pipette volume, test numbers, test dates and pipette information (manufacturer, model,
serial number) can be recorded as test data. The test data, output to a printer or stored in the PC,
allows easy management of pipette accuracy and reduces errors due to data mismanagement.
The specifications of accuracy and repeatability can be entered either as an absolute value (μL)
or as a relative value (percentage to the pipette volume).
The mass value data from the balance can be transmitted to the PC either using a COM port or
using a USB connection. (For a USB connection, use the accessory USB converter.)
For testing various pipettes easily, multiple test conditions (pipette volume, number of
measurements and specifications) can be set previously. When multiple operators are set
previously, an operator can be selected easily as necessary.
4
NOTE For detailed information on WinCT-Pipette, refer to “How to use WinCT-Pipette.pdf” in the
f
WinCT-Pipette CD-ROM.
Main Window
When WinCT-Pipette starts up, the window below opens
Specifications
Enter the pipette volume, specifications of
accuracy and repeatability used for
pass/fail judgment.
Measured Values
Displays the mass values transmitted from the
balance and the volumetric values obtained
using the Z factor.
Measurement Results
Displays the measurement results and
judgment results.
The Z factor is automatically calculated using the values o
the distilled water temperature and the barometric pressure.
Measurement environment
Enter the ambient relative humidity,
the distilled water temperature and
the barometric pressure.
1-4 Function of the Evaporation Trap
In the pipette accuracy test using the gravimetric method, to obtain the mass of the test liquid, distilled
water is dispensed from the pipette into the sample cup installed on the balance. The obtained value,
however, is generally smaller due to evaporation loss of the test liquid. To prevent evaporation of the test
liquid, the evaporation trap is used. The ISO 8655 standard recommends that the humidity of the
measurement environment be greater than 50% RH.
Water is poured into the groove of the evaporation trap. This helps to maintain the humidity inside
the evaporation trap high and reduces evaporation. Using the evaporation trap reduces the
evaporation of the test liquid to about 25% or 0.05 mg per minute, compared with the value when the
evaporation trap is not used. This yields a measurement environment where there is no need to
consider measurement errors due to evaporation loss of the test liquid.
Another function of the evaporation trap is one as a breeze break, to prevent drafts in the
measurement environment from influencing the weighing operation and enable a stable weighing.
The evaporation trap is designed for an easy dispensing of the test liquid. The upper part has a
sloped surface so that the pipette can be inserted from obliquely above.
5
A
2. ASSEMBLING THE TESTER
2-1 AD-4212B-PT / AD-4212A-PT
1. Place the weighing unit on a solid table. For details
on the installation site, refer to “3. PRECAUTIONS
BEFORE USE”.
Secure the display to the stand using the angle
adjustment knobs. Connect the display and the
weighing unit using the connection cable.
2. Install the evaporation trap base on the weighing
unit.
Stand
Evaporation
trap
base
Display
Connection
cable
Weighing
unit
ngle
adjustment
knob
3. Install the sample cup holder (30 mL) on the base.
When the 30 mL sample cup is used
Insert the sample cup into the holder.
When the 5 mL sample cup is used
Insert the sample cup holder (5 mL) into
the sample cup holder (30 mL), and then
insert the sample cup
:
:
5 mL sample
cup
Sample cup
holder (5 mL)
Sample cup
holder (30 mL)
30 mL sample
cup
6
4. Install the lower part of the evaporation trap on the
weighing unit, mating the tabs with the holes on the
base.
5. Attach the upper part of the evaporation trap to the
lower part, mating the tab with the notch.
Lower part of
the evaporation trap
Tab
Hole
Upper part of
the evaporation trap
Tab
Lower part of
the evaporation trap
Notch
6. Adjust the direction of the evaporation trap so that the pipette insertion opening on the sloped
surface is faced to allow easy operation.
Pipette
insertion
opening
of the weighing unit
右 左
Facing to the right
Facing to the left
of the weighing unit
Pipette
insertion
opening
Cap A
7. Install cap A on the evaporation trap.
Remove cap B from the sloped surface.
Upper part of
the evaporation trap
Cap B
7
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