3M 1210 User Manual
Description
Electronic Pipettor - 5mL
Each unit includes the following items:
Pipette Electronique - 5mL
Elektronische Pipette - 5mL
Pipetta Elettronica - 5mL
Pipeteador electrónico - 5mL
•1 Electronic Pipettor
•1 Charging Stand
•1 AC Adapter
•1 Test Performance Certificate
•1 Instruction Manual
•1 Quick Reference Card
•Replacement Tip Cone Filters (50/bag)
Page 1 of 15 - June 2001
charging stand pipettor
5mL
Make sure that all items are included
and that no damage has occurred during
shipment. Also check that the AC adapter matches your electrical requirements.
Pipettor Controls
These controls are often referred to in this manual.
Speed Key Changes aspiration and dispense speeds
Mode Key Shifts to next mode
Up Key Increases open volume or speed
Down Key Decreases open volume or speed
Recall Key Flips back to last mode entered
Enter Key Selects mode,speed or volume changes
Start Button Triggers all mechanical operations
Tip Ejector Lever Squeezed to remove pipette tip
ON-OFF SWITCH
The On-Off Switch is located at the
back of the pipettor. Slide to the left
position to switch "ON" and to the
right to switch "OFF".
AC adapter
keypad
display
start button
tip ejector
lever
6483
FOD# 1210
ON
OFF
tip ejector
collar
tip cone
filter
2
Precautions
4
• Always follow Good Laboratory Practices.
• Avoid contamination of the tip cone. Do not rest the pipettor on its side. Always
place the pipettor in the charging stand when not in use.
• Ensure consistent technique to maintain precise results.
• Never strike the pipettor tip cone against the tip tray when mounting tips. This
can damage internal components.
• Avoid excessive shock to pipettor. Do not drop the pipettor, charging stand or AC
adapter.
• Avoid exposing the unit to extreme temperature changes, humidity and dust.
• Avoid rough handling when using the unit. Moderate keystroke pressure is all that
is required for all operating buttons.
Indications
3
The easy-to-use 3M Electronic Pipettor - 5mL is pre-programmed to perform the
most common dilutions used with 3M
™
Petrifilm™Plates and 3M™Redigel™Tests.
The self-calibrating, microprocessor-based system reduces the possibility of human
error and instrument contamination by controlling pipetting speed, calibration and
volume selection. It is a simple yet sophisticated instrument for handling microvolumes of liquids with high accuracy and precision. Its light-weight and ergonomic
controls take the effort out of pipetting to help reduce the risk of repetitive strain
injuries that are frequent in manual pipetting.
The 3M Electronic Pipettor 5mL includes:
• Pre-programmed diluting, pipetting, and dispensing modes
• Self-calibrating, microprocessor-controlled system
• Full-grip; low-effort tip ejector
• Soft-touch operating button
• Open programming mode for user-set pipetting and diluting
• Recall button for selecting previous mode
• LCD screen for viewing speed, mode and volume
• 5 speeds for aspiration and dispensing
• Cordless operation
• Magnetic-mount recharging stand
• Replaceable NiMH battery
• Weighs less than 6 ounces
• Standard Methods for the Examination of Dairy Products (SMEDP) accuracy
Page 2 of 15 - June 2001
Instructions For Use
6
1. Connect the AC adapter into the connector jack at the rear of the charging
stand's base.
2. Plug the AC adapter into an AC electrical outlet.
3. The green light at the top of the charging stand indicates that the stand is in
operation. The light will always be on when plugged in and operating properly.
Charging the Pipettor Battery
1. Switch the pipettor “ON” (see page 2).
2. Mount the pipettor into the charging stand. The top of the pipettor will fit snugly
into the head of the charging stand. The magnetic mount will lock the pipettor into
the proper charging position.
3. If the battery is new or has been allowed to fully discharge, keep the pipettor in
the stand for 10 hours to fully charge the battery before continuing use.
• The unit will charge when “ON” or “OFF”. Leave the pipettor “ON” and in the
charging stand when not in use to retain user-selected pipettor settings.
• Switching the unit “OFF”clears user-set programming and resets the pipettor to
default settings.
Instructions For Use
5
Only use and store the pipettor in accordance with the following
operating conditions:
Condition Range or Limit
Performance Testing Temperature +19 to + 25°C
Mechanical Operating Temperature +15 to + 35°C
Storage Temperature -20 to + 40°C
Maximum Humidity 95%
The following recommendations should always be followed for best results:
1. During aspiration, hold the pipettor vertically. For greatest accuracy, the pipettor
should never be tilted at an angle greater than 15° during aspiration.
2. During aspiration, consistently place the tip no deeper than 5mm into the liquid.
3. During dispensing, position the tip at least 5mm above the surface to prevent
bubbles from forming.
Connecting the AC Adapter
• Be sure that the voltage rating of the AC adapter correctly corresponds to the
voltage from the electrical outlet.
• Do not use electrical outlets that are timed or switched.
• Use only the AC adapter provided with the unit or an approved replacement
from 3M.
1.
2.
Page 3 of 15 - June 2001
Power “ON” Display and Startup
The display of a switched “ON” pipettor indicates that programming has been reset
to default settings.
1. Switch the unit “ON”. Display:
2. Press the Start Button twice. The display
shows that the unit is ready for the
default 1:5 dilution (Mode d1, 5mL) or
for program changes.
Switching the pipettor “OFF” and then
“ON” will reset the unit to the default
settings (Mode d1, 1:5, 5mL dilution).
Mode Selection and Mode Recall
Selecting available modes on the pipettors is done with the use of these three keys:
1. Press once to display the default dilution mode of the pipettor.
2. Press repeatedly to view each of the modes available.
3. Press when the desired mode is displayed. This will enter the mode
selection and allow you to use this mode.
Mode recall allows you to flip back and forth between the last two
modes used.
or may not be used in the middle of an aspiration or
dispensing cycle.
Pipettor Programming
8
Pipettor Programming
7
The Program Menu
1. The following five dilutions have been pre-programmed.
Dilution Diluent Sample Total
Mode Ratio Volume Volume Volume
d1 1:5 4.00mL 1.00mL 5.00mL
d2 1:10 4.50mL 0.50mL 5.00mL
d3 1:20 4.75mL 0.25mL 5.00mL
d4 1:5 0.80mL 0.20mL 1.00mL
d5 1:10 0.90mL 0.10mL 1.00mL
2. Repeat Dispense 1mL ModeThis mode performs repeat 1mL dispensing.
Mode Volume Repetitions
rd 1.00mL 1-5
3. Pipette Modes These modes perform standard 1mL and 5mL pipetting with blow-out.
Mode Volume
P1 1.00mL
P5 5.00mL
4. Open Dilution Mode This mode allows selection of any dilution ratio within the range of the pipettor
Diluent Sample Max.
Volume Volume Total
Mode Increment Range Range Volume
d0 0.05mL 0.10-5.00mL 0.00-4.90mL. 5.00mL
Page 4 of 15 - June 2001
1:10 Dilution (5mL Total Volume)
1. Press repeatedly until the
display reads:
2. Press to enter selection.
3. Attach a pipette tip to the tip cone.
The pipettor is now ready to begin the
1:10 dilution (5mL total volume). The
display indicates that the pipettor is
ready to aspirate 4.50mL.
4. Place the tip in the diluent and press the
Start Button. The unit will aspirate
4.50mL.
The following display indicates that the
unit is ready to aspirate 0.50mL.
5. Place the tip in the sample solution
and press the Start Button to aspirate
0.50mL.
The next display indicates that the unit
is ready to dispense the total volume
(5mL) with blowout.
6. Position the pipette tip to dispense and
press the Start Button. The unit will
dispense the full amount from the tip.
The display now indicates that the unit
is ready to repeat the dilution sequence,
beginning with the 4.50mL aspiration.
10
Pipettor Programming
1:5 Dilution (5mL Total Volume)
1. Press repeatedly until the
display reads:
2. Press to enter selection.
3. Attach a pipette tip to the tip cone.
The pipettor is now ready to begin the
1:5 dilution (5mL total volume). The
display indicates that the pipettor is
ready to aspirate 4.00mL.
4. Place the tip in the diluent and press the
Start Button. The unit will aspirate
4.00mL.
The following display indicates that the
unit is ready to aspirate 1.00mL.
5. Place the tip in the sample solution
and press the Start Button to aspirate
1.00mL.
The next display indicates that the unit
is ready to dispense the total volume
(5mL) with blowout.
6. Position the pipette tip to dispense
and press the Start Button. The unit will
dispense the full amount from the tip.
The display now indicates that the unit
is ready to repeat the dilution sequence,
beginning with the 4.00mL aspiration.
9
Pipettor Programming
Page 5 of 15 - June 2001
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