3B Scientific 500 LED User Manual

3B SCIENTIFIC® PHYSICS

Binocular Microscope Model 500 LED with Polarisation

Equipment 1013146

Instruction Manual

07/13 ALF

1 Eyepiece
2 Tube
3 Slot for inserting the analy-

ser
4 Revolver with objectives
5 Object guide
6 Object stage
7 Condensor with iris diaph-

ragm and filter holder
8 Lamp housing
9 Illumination control
10 Mains switch
11 1Coaxial movement control

for the specimen stage
12 Coarse and fine movement

controls with holding brake
13 Lock screw for object stage
14 Stand
15 Head lock screw

1. Safety notes

• For power supply use only electrical sockets

with ground contact.

2. Description, technical data

The binocular microscope allows two­dimensional viewing of objects (thin sections of
plant or animal specimen) in 40x to 1500x mag­nification. It is fitted with polarisation equipment.

Stand: Robust, all metal stand with arm perma­nently connected to the base. Focussing by
means of separate knobs for coarse and fine
adjustment located on either side of the stand
and operated by rack and pinion drive with ball
bearings and retaining lever, adjustable stopper
for protecting the object slides and objective.

Focus range: 15mm, resolution of fine focussing
adjustment: 0.002 mm

Tube: Binocular Siedentopf head, 30° viewing
angle, 360° rotatable head, viewing distance
adjustable between 54 and 75 mm, ±5 dioptric
compensation for both eyepieces

Polarisation equipment: Polariser and ana­lyser

Eyepiece: Pair of wide field eyepieces WF 10x
18 mm and WF 15x 13 mm

Objectives: Inverted objective revolver with 4
plan achromatic objectives 4x / 0.10, 10x / 0.25,
40x / 0.65, 100x / 1.25 (oil)

Magnification: 40x – 1500x
Object stage: x-y cross table, 155 x 145 mm

with object guide and coaxial adjustment knobs
perpendicular to the object stage, adjustment
range 50 x 76 mm

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2

2

,

Illumination: Adjustable LED lighting integrated
in base, universal 85 to 265 V, 50/60 Hz power
supply

Condenser: Abbe condenser N.A.1.25 NA 0.65
with iris diaphragm , filter holder and blue filter,
focussed via rack and pinion drive

Dimensions: 306 x 190 x 407 mm³ approx.
Weight: 6.6 kg approx.

3. Unpacking and assembly

The microscope is packed in a molded styro­foam container.

• Take the container out of the carton remove

the tape and carefully lift the top half off the
container. Be careful not to let the optical
items (objectives and eyepieces) drop down.

• To avoid condensation on the optical com-

ponents, leave the microscope in the original
packing to allow it to adjust to room tem­perature.

• Using both hands (one around the pillar and

one around the base), lift the microscope
from the container and put it on a stable
desk.

• The objectives will be found within individual

protective vials. Install the objectives into the
microscope nosepiece from the lowest
magnification to the highest, in a clockwise
direction from the rear.

• Put the head onto the top of the stand and

tighten the head-lock-screw. Insert the eye­pieces into the tube.

4. Operation

4.1 General information

• Set the microscope on a level table.

• Place the object to be observed in the centre

of the specimen stage and clamp it to the
object guide.

• Connect the mains cable to the net and turn

on the switch to get the object illuminated.

• Make certain that the specimen is centered

over the opening in the stage.

• Adjust the interpupillary distance so that one

circle of light can be seen.

• Make the necessary eyepiece dioptre ad-

justments to suit your eyes.

• To obtain a high contrast, adjust the back-

ground illumination by means of the iris dia­phragm and the variable illumination control.

• Rotate the nosepiece until the objective with

the lowest magnification is pointed at the

specimen. There is a definite “click” when
each objective is lined up properly.

NOTE: It is best to begin with the lowest power
objective. This is important to reveal general
structural details with the largest field of view
first. Than you may increase the magnification
as needed to reveal small details. When 100x

(oil) objective is chosen, objective oil must be
dripped onto the slide.

To determine the magnification at which you are
viewing a specimen, multiply the power of the
eyepiece by the power of the objective.

• Adjust the holding brake to give a suitable

degree of tightness in the focusing mecha­nism.

• Adjust the coarse-focusing-knob which

moves the stage up until the specimen is fo­cused. Be careful that the objective does not
make contact with the slide at any time. This
may cause damage to the objective and/or
crack your slide.

• Adjust the fine-focusing-knob to get the im-

age more sharp and more clear.

• Colour filters may be inserted into the filter

holder for definition of specimen parts.
Swing the filter holder out and insert colour
filters.

• Use the knobs of the mechanical stage to

move the slide side-, back- and forwards.
The vernier provides acc urate loc ation of the
specimen area.

• Always turn off the light immediately after

use.

• Be careful not to spill any liquids on the mi-

croscope.

• Do not mishandle or impose unnecessary

force on the microscope.

• Do not wipe the optics with your hands.

• Do not attempt to service the microscope

yourself.

4.2 Using the polarisation equipment

• Insert the analyser into the slot on the re-

volving nosepiece.

• Place the polarising filter on the rim aperture

of the light source.

• Rotate the polariser until the planes of the

polariser and the analyser are exactly
crossed, so that one sees a black back­ground.

Any object with a doubly-refracting (birefringent)
structure should now appear brightly illuminated
against the dark background. If that does not
occur, it is possible that the direction of light
vibration of the object coincides with the polari­sation direction. Whether or not that is the case

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