3B Scientific 400 User Manual
3B SCIENTIFIC® PHYSICS
Trinocular Microscope Model 400 1003276
Instruction Manual
03/13 ALF
1. Safety notes
• For power supply use only electrical sockets
with ground contact.
Caution! The Stirling engine becomes hot during
use. Risk of burns!
• Do not touch the lamp during or immediately
after use.
2. Description, technical data
The trinocular microscope allows two observers
to have a two-dimensional view of objects (thin
sec-tions of plant or animal specimen) at a
magnification of 40 to 1500 times. It also allows
a camera to be attached at the same time for
photographic or video-recording documentation
of images.
Stand: Robust, all metal stand with arm permanently connected to the base. Focussing by
means of separate knobs for coarse and fine
adjustment located on either side of the stand
and operated by rack and pinion drive with ball
bearings and retaining lever, adjustable stopper
for protecting the object slides and objective.
Focus range: 15mm, resolution of fine focussing
adjustment: 0.002 mm
Tube: Trinocular Siedentopf head, 360° rotatable, binocular tubus with 30° viewing angle,
viewing distance adjustable between 54 and
75 mm, ±5 dioptric compensation for both eyepieces, one tube with vertical viewing angle
Eyepiece: Pair of wide field eyepieces WF 10x
18 mm and WF 15x 13 mm
Objectives: Revolver with 4 DIN achromatic
objectives 4x / 0.10, 10x / 0.25, 40x / 0.65, 100x
/ 1.25 oil (with specimen protection)
Magnification: 40x – 1500x
Object stage: x-y cross table, 132 x 145 mm
with object guide and coaxial adjustment knobs
1 Eyepiece
2 Tube
3 Revolver with objectives
4 Object stage
5 Condensor lock screw
6 Condensor with iris diaphragm
and filter holder
7 Lamp housing
8 Illumination control
9 Coarse and fine movement con-
trols with holding brake
10 Coaxial movement control for the
specimen stage
11 Mains switch
12 Object guide
13 Lock screw for object stage
14 Stand
15 Head lock screw
2
,
1
perpendicular to the object stage, adjustment
range 50 x 76 mm
2
Illumination: Adjustable 6 V, 20 W halogen
lamp incorporated into the base, universal 85 to
265 V, 50/60 Hz power supply
Condenser: Abbe condenser N.A.1.25 NA 0.65
with iris diaphragm, filter holder and blue filter,
focussed via rack and pinion drive
Dimensions: 328 x 214 x 449 mm³ approx.
Weight: 6.2 kg approx.
3. Unpacking and assembly
The microscope is packed in a molded styrofoam container.
• Take the container out of the carton remove
the tape and carefully lift the top half off the
container. Be careful not to let the optical
items (objectives and eyepieces) drop down.
• To avoid condensation on the optical com-
ponents, leave the microscope in the original
packing to allow it to adjust to room temperature.
• Using both hands (one around the pillar and
one around the base), lift the microscope
from the container and put it on a stable
desk.
• The objectives will be found within individual
protective vials. Install the objectives into the
microscope nosepiece from the lowest
magnification to the highest, in a clockwise
direction from the rear.
• Put the head onto the top of the stand and
tighten the head-lock-screw. Insert the eyepieces into the tube.
4. Operation
4.1 General information
• Set the microscope on a level table.
• Place the object to be observed in the centre
of the specimen stage and clamp it to the
object guide.
• Connect the mains cable to the net and turn
on the switch to get the object illuminated.
• Make certain that the specimen is centered
over the opening in the stage.
• To obtain a high contrast, adjust the back-
ground illumination by means of the iris diaphragm and the variable illumination control.
• Adjust the interpupillary distance so that one
circle of light can be seen.
• Make the necessary eyepiece dioptre ad-
justments to suit your eyes.
• Rotate the nosepiece until the objective with
the lowest magnification is pointed at the
specimen. There is a definite “click” when
each objective is lined up properly.
NOTE: It is best to begin with the lowest power
objective. This is important to reveal general
structural details with the largest field of view
first. Than you may increase the magnification
as needed to reveal small details. When 100x
(oil) objective is chosen, objective oil must be
dripped onto the slide.
To determine the magnification at which you are
viewing a specimen, multiply the power of the
eyepiece by the power of the objective.
• Adjust the holding brake to give a suitable
degree of tightness in the focusing mechanism.
• Adjust the coarse-focusing-knob which
moves the stage up until the specimen is focused. Be careful that the objective does not
make contact with the slide at any time. This
may cause damage to the objective and/or
crack your slide.
• Adjust the fine-focusing-knob to get the im-
age more sharp and more clear.
• Colour filters may be inserted into the filter
holder for definition of specimen parts. Swing
the filter holder out and insert colour filters.
• Use the knobs of the mechanical stage to
move the slide side-, back- and forwards.
The vernier provides acc urate loc ation of the
specimen area.
• Always turn off the light immediately after
use.
• Be careful not to spill any liquids on the mi-
croscope.
• Do not mishandle or impose unnecessary
force on the microscope.
• Do not wipe the optics with your hands.
• Do not attempt to service the microscope
yourself.
4.2 Changing the lamp and fuse
4.2.1 Changing the lamp
• Turn off the power switch, unplug the mains
plug and let the lamp cool down to avoid being burnt.
• For safety reasons, remove the eyepiece.
• To change the lamp lay the microscope on
its back to reach the lid on the bottom side.
• Loosen screw C of the lamp socket and
push it outwards so that it is in the position
shown in Fig.1.
• Loosen screw A and open the cover.
• To remove the halogen lamp, use a cloth or
similar material. Do not touch the bulb with
2
Loading...