Waters Xevo TQ-S micro Overview And Maintenance Manual

Waters Xevo TQ-S micro

Overview and Maintenance Guide

715004599/ Revision B

Copyright © Waters Corporation 2014 – 2016

All rights reserved

ii January 11, 2016, 715004599 Rev. B

General Information

Copyright notice

© 2014 – 2016 WATERS CORPORATION. PRINTED IN THE UNITED STATES OF
AMERICA AND IN IRELAND. ALL RIGHTS RESERVED. THIS DOCUMENT OR
PARTS THEREOF MAY NOT BE REPRODUCED IN ANY FORM WITHOUT THE
WRITTEN PERMISSION OF THE PUBLISHER.

The information in this document is subject to change without no tice and should not be
construed as a commitment by Waters Corporation. Waters Corporation assumes no
responsibility for any errors that may appear in this document. This document is believed
to be complete and accurate at the time of publication. In no event shall Waters
Corporation be liable for incidental or consequential damages in connection with, or
arising from, its use. For the most recent revision of this document, consult the Waters
Web site (waters.com).

Trademarks

ACQUITY, ACQUITY UltraPerformance LC, ACQUITY UPLC, Alliance, Connections
INSIGHT , ESCi, MassLynx, “THE SCIENCE OF WHAT’S POSSIBLE”, UPLC, Waters,
Waters Quality Parts, and Xevo are registered trademarks of Waters Corporation, and
iKey, IntelliStart, ionKey, ionKey/MS, IonSABRE, NanoFlow, RADAR, T-Wave, and
ZSpray are trademarks of Waters Corporation.

DART is a registered trademark of JEOL USA Inc.
GELoader is a registered trademark of New Brunswick Scientific, Co., Inc.
LDTD is a trademark of Phytronix Technologies Inc.
PEEK is a trademark of Victrex Corporation.
Phillips and Pozidriv are registered trademarks of Phillips Screw Company, Inc.
snoop and Swagelok are registered trademarks of Swagelok Company.
Spark Holland and Symbiosis are trademarks of Spark-Holland BV.
Viton is a registered trademark of DuPont.
Other registered trademarks or trademarks are the sole property of their owners.

January 11, 2016, 715004599 Rev. B iii

Customer comments

Waters’ Technical Communications organization invites you to report any errors that you
encounter in this document or to suggest ideas for otherwise improving it. Help us better
understand what you expect from our documentation so that we can continuously improve
its accuracy and usability.

We seriously consider every customer comment we receive. You can reach us at
[e-mail].

Contacting Waters

Contact Waters® with enhancement requests or technical questions regarding the use,
transportation, removal, or disposal of any Waters product. You can reach us via the
Internet, telephone, or conventional mail.

Waters contact information:

Contacting medium Information

Internet The Waters Web site includes contact information for

Telephone and fax From the USA or Canada, phone [phone], or

Waters locations worldwide. Visit www.waters.com.

fax [phone].
For other locations worldwide, phone and fax

numbers appear in the Waters Web site.

Conventional mail Waters Corporation

34 Maple Street
Milford, MA 01757
USA

Safety considerations

Some reagents and samples used with Waters instruments and devices can pose chemical,
biological, or radiological hazards (or any combination thereof). You must know the
potentially hazardous effects of all substances you work with. Always follow Good
Laboratory Practice, and consult your organization’s standard operating procedures.

iv January 11, 2016, 715004599 Rev. B

Safety hazard symbol notice

Documentation needs to be consulted in all cases where the symbol is used to find
out the nature of the potential hazard and any actions which have to be taken.

Considerations specific to the Xevo TQ-S micro

Power cord replacement hazard

Warning: To avoid electric shock, use the SVT-type power cord in the United

States and HAR-type (or better) in Europe. The main power cord must only be
replaced with one of adequate rating. For information regarding what cord to use in
other countries, contact your local Waters distributor.

Solvent leakage hazard

The source exhaust system is designed to be robust and leak-tight. Waters recommends
you perform a hazard analysis, assuming a maximum leak into the laboratory atmosphere
of 10% LC eluate.

Warning:

• To confirm the integrity of the source exhaust system, renew the source
O-rings at intervals not exceeding one year.

• To avoid chemical degradation of the source O-rings, which can
withstand exposure only to certain solvents (see page 258), determine
whether any solvents you use that are not listed are chemically
compatible with the composition of the O-rings.

January 11, 2016, 715004599 Rev. B v

Spilled solvent s hazard

Prohibited: To avoid injury or equipment damage caused by spilled solvent, do

not place reservoir bottles on top of the instrument or on its front ledge.

Flammable solvents hazard

Warning: To prevent ignition of flammable solvent vapors in the enclosed space

of a mass spectrometer’s ion source, ensure that nitrogen flows continuously
through the source. The nitrogen supply pressure must not fall below 400 kPa
(4 bar, 58 psi) during an analysis requ iring the use of flammable solvents. Also a
gas-fail device must be installed, to interrupt the flow of LC solvent should the
nitrogen supply fail.

When using flammable solvents, ensu re that a stream of nitrogen continuously flushes the
instrument’s source, and the nitrogen supply pressure remains above 400 kPa (4 bar,
58 psi). You must also install a gas-fail device that interrupts the solvent flowing from the
LC system in the event the supply of nitrogen fails.

Overload hazard

Warning: To prevent personal injury, ensure equipment placed on top of the Xevo

TQ-S micro does not exceed 15kg.

vi January 11, 2016, 715004599 Rev. B

Glass-breakage hazard

Source enclosure assemblySource enclosure assembly

Warning: To avoid injuries from broken glass, falling objects, or

exposure to toxic or biohazardous substances, never place containers
on top of the instrument or on its front covers.

High temperature hazard

Warning: To avoid burn injuries, ensure the source heater is turned off and the ion

block is cool before performing maintenance on these components. The source ion
block, located behind the source enclosure assembly, can become hot.

Xevo TQ-S micro high temperature hazard:

January 11, 2016, 715004599 Rev. B vii

Hazards associated with removing an instrument from service

Warning: To avoid personal contamination with biohazards, toxic

materials, or corrosive materials, wear chemical-resistant gloves
during all phases of instrument decontamination.

Warning: To avoid puncture injuries, handle syringes, fused silica

lines, and borosilicate tips with extreme care.

When you remove the instrument from use to repair or dispose of it, you must
decontaminate all of its vacuum areas. These are the areas in which you can expect to
encounter the highest levels of contamination:

• Source interior

• Waste tubing

• Exhaust system

• Rotary pump oil (where applicable)

The need to decontaminate other vacuum areas of the instrument depends on the kinds of
samples the instrument analyzed and their levels of concentration. Do not dispose of the
instrument or return it to Waters for repair until the authority responsible for approving its
removal from the premises specifies the extent of decontamination required and the level
of residual contamination permissible. That authority must also prescribe the method of
decontamination to be used and the appropriate protection for personnel undertaking the
decontamination process.

You must handle items such as syringes, fused silica lines, and borosilicate tips used to
carry sample into the source area in accordance with laboratory procedures for
contaminated vessels and sharps. To avoid contamination by carcinogens, toxic
substances, or biohazards, you must wear chemical-resistant gloves when handling or
disposing of used oil.

Bottle placement prohibition

Prohibited: To avoid injury from electric shock or fire, and to prevent damage to

the workstation and ancillary equipment, do not place objects filled with
liquid—such as solvent bottles—on these items, or expose them to dripping or
splashing liquids.

viii January 11, 2016, 715004599 Rev. B

FCC radiation emissions notice

Changes or modifications not expressly approved by the party responsible for compliance,
could void the users authority to operate the equipment. This device complies with Part 15
of the FCC Rules. Operation is subject to the following two conditions: (1) this device
may not cause harmful interference, and (2) this device must accept any interference
received, including interference that may cause undesired operation.

Electrical power safety notice

Do not position the instrument so that it is difficult to operate the disconnecting device.

Equipment misuse notice

If the equipment is used in a manner not specified by the manufacturer, the protection
provided by the equipment may be impaired.

Safety advisories

Consult Appendix A for a comprehensive list of warning advisories and notices.

January 11, 2016, 715004599 Rev. B ix

Operating this instrument

When operating this instrument, follow standard quality-control (QC) procedures and the
guidelines presented in this section.

Applicable symbols

Symbol Definition

Manufacturer

Date of manufacture

Part number catalog number

5()

Serial number

Supply ratings

Authorized representative of the European Community

Confirms that a manufactured product complies with all
applicable European Community directives

Australia EMC Compliant

or

Confirms that a manufactured product complies with all
applicable United States and Canadian safety requirements

x January 11, 2016, 715004599 Rev. B

Symbol Definition

Consult instructions for use

Electrical and electronic equipment with this symbol may
contain hazardous substances and should not be disposed
of as general waste.

For compliance with the Waste Electrical and Electronic
Equipment Directive (WEEE) 2012/19/EU, contact W aters
Corporation for the correct disposal and recycling
instructions.

January 11, 2016, 715004599 Rev. B xi

Audience and purpose

This guide is for operators of varying levels of experience. It gives an overview of the
instrument and explains how to prepare it for operation, switch between modes of
operation, and maintain it.

Intended use of the Xevo TQ-S micro

Waters designed the Xev o TQ-S mic r o for use as a research tool to accurately,
reproducibly, and robustly quantify target compounds present at the lowest possible levels
in highly complex sample matrices. The Xevo TQ-S micro is not intended for use in
diagnostic applications.

Calibrating

To calibrate LC systems, follow accepta ble calibration methods using at least five
standards to generate a standard curve. The concentration range for standards should
include the entire range of QC samples, typical specimens, and atypical specimens.

When calibrating mass spectrometers, consult the instrument’s online Help system for
instructions.

Quality control

Routinely run three QC samples that represent subnormal, normal, and above-normal
levels of a compound. If sample trays are the same or very similar, vary the location of the
QC samples in the trays. Ensure that QC sample results fall within an acceptable range,
and evaluate precision from day to day and run to run. Data collected when QC samples
are out of range might not be valid. Do not report these data until you are certain that the
instrument performs satisfactorily.

When analyzing samples from a complex matrix such as soil, tissue, serum/plasma, whole
blood, and other sources, note that the matrix components can adversely affect LC/MS
results, enhancing or suppressing ionization. To minimize these matrix effects, adopt the
following measures:

• Prior to the instrumental analysis, use appropriate sample pretreatment such as
protein precipitation, liquid/liquid extraction (LLE), or solid phase extraction (SPE)
to remove matrix interferences.

• Whenever possible, verify method accuracy and precision using matrix-matched
calibrators and QC samples.

xii January 11, 2016, 715004599 Rev. B

• Use one or more internal standard compounds, preferably isotopically labeled
analytes.

EMC considerations

Canada spectrum management emissions notice

This class A digital product apparatus complies with Canadian ICES-001.
Cet appareil numérique de la classe A est conforme à la norme NMB-001.

ISM Classification: ISM Group 1 Class A

This classification has been assigned in accordance with CISPR 11 Industrial Scientific
and Medical, (ISM) instrument requirements. Group 1 products apply to intentionally
generated and/or used conductively coupled radio-frequency energy that is necessary for
the internal functioning of the equipment. Class A products are suitable for use in all
establishments other than residential locations and those directly connected to a low
voltage power supply network supplying a building for domestic purposes.

There may be potential difficulties in ensuring electromagnetic compatibility in other
environments due to conducted as well as radiated disturbances.

Do not use the equipment in close proximity to sources of strong electromagnetic radiation
(for example, unshielded intentional RF sources), as these may interfere with the
equipment’s proper operation.

This equipment complies with the emission and immunity requirements described in the
relevant parts of IEC/EN 61326: Electrical equipment for measurement, control and

laboratory use — EMC requirements.

January 11, 2016, 715004599 Rev. B xiii

EC authorized representative

Waters Corporation
Stamford Avenue
Altrincham Road
Wilmslow
SK9 4AX
United Kingdom

Telephone: [phone]
Fax: [phone]
Contact: Quality manager

xiv January 11, 2016, 715004599 Rev. B

Table of Contents

Copyright notice ..................................................................................................................... iii

Trademarks ............................................................................................................................. iii

Customer comments ................................................ .............................................................. iv

Contacting Wa ters ................................................................................................................. iv

Safety considerations ............................................................................................................. iv

Safety hazard symbol notice............................................................................................... v

Considerations specific to the Xevo TQ-S micro............................................................... v

FCC radiation emissions notice......................................................................................... ix

Electrical power safety notice............................................................................................ ix

Equipment misuse notice................................................................................................... ix

Safety advisories................................................................................................................ ix

Operating this instrument ...................................................................................................... x

Applicable symbols ............................................................................................................ x

Audience and purpose....................................................................................................... xii

Intended use of the Xevo TQ-S micro.............................................................................. xii

Calibrating ........................................................................................................................ xii

Quality control.................................................................................................................. xii

EMC considerations ............................................................................................................. xiii

Canada spectrum management emissions notice............................................................. xiii

ISM Classification: ISM Group 1 Class A...................................................................... xiii

EC authorized representative ............................................................................................. xiv

1 Specifications and Operating Modes ....................................................................... 23

Uses and compatibility ......................................................................................................... 24

ACQUITY Xevo TQ-S micro UPLC/MS systems........................................................... 26

Software and data system ................................................................................................. 29

Ionization techniques and source probes ........................................................................... 31

Electrospray ionization (ESI) ........................................................................................... 31

January 11, 2016, 715004599 Rev. B xv

Combined ESI and APCI (ESCi)...................................................................................... 31

Atmospheric pressure chemical ionization (APCI).......................................................... 31

Dual-mode APPI/APCI source......................................................................................... 31

NanoFlow source.............................................................................................................. 32

Atmospheric solids analysis probe (ASAP) ..................................................................... 33

Atmospheric pressure gas chromatography (APGC) ....................................................... 33

ionKey source................................................................................................................... 33

IntelliStart Fluidics system .............................................................................. ..... ............... 34

Functionality..................................................................................................................... 34

System operation .............................................................................................................. 35

Ion optics ............................................................................................................................... 36

MS operating modes ............................................................................................................ 36

MS/MS operating modes ..................................................................................................... 38

Product (daughter) ion mode............................................................................................ 39

Precursor (parent) ion mode ............................................................................................. 40

Multiple reaction monitoring mode.................................................................................. 40

Constant neutral loss mode............................................................................................... 42

Sample inlet ............................................ .... ..... ..................................................................... 43

Leak sensors ...................................................................... .... ............................................... 43

Vacuum system ..................................................................................................................... 43

Rear panel connections ........................................................................................................ 44

2 Preparing for Operation ........................................................................................... 45

Starting the mass spectrometer ........................................................................................... 46

Verifying the instrument’s state of readiness.................................................................... 49

Monitoring the instrument LEDs...................................................................................... 49

Tuning and calibration information.................................................................................. 49

Running the instrument at different flow rates................................................................. 50

Preparing the IntelliStart Fluidics system ......................................................................... 51

Installing the reservoir bottles .......................................................................................... 51

Purging the infusion pump................................................................................................ 53

xvi January 11, 2016, 715004599 Rev. B

Rebooting the instrument .................................................................................................... 53

Leaving the mass spectrometer ready for operation ........................................................ 54

Emergency shutdown of the mass spectrometer............................................................... 54

3 Changing the Mode of Operation ............................................................................ 55

ESI mode ............................................................................................................................... 56

Installing the ESI probe.................................................................................................... 56

Removing the ESI probe................................................................................................... 59

ESCi mode ............................................................................................................................ 60

Optimizing the ESI probe for ESCi operation.................................................................. 60

APCI mode ........................................................................................................................... 60

Installing the IonSABRE II probe .................................................................................... 61

Removing the IonSABRE II probe................................................................................... 64

Combined APPI/APCI source ............................................................................................. 65

APPI operation.................................................................................................................. 65

APCI operation................................................................................................................. 66

Dual-mode operation ........................................................................................................ 67

The combined APPI/APCI source components................................................................ 68

Installing the combined APPI/APCI source..................................................................... 70

Removing the IonSABRE II probe and APPI/APCI source enclosure ............................ 71

NanoFlow source .................................................................................................................. 72

Installing the NanoFlow source........................................................................................ 73

Fitting a borosilicate glass capillary (nanovial)................................................................ 76

Positioning the borosilicate glass capillary tip................................................................. 79

ionKey source ....................................................................................................................... 80

Installing the ionKey source............................................................................................. 80

Installing ionKey source software.................................................................................... 85

Installing the camera in the ionKey source....................................................................... 86

Removing the ionKey source............................................................................................ 86

January 11, 2016, 715004599 Rev. B xvii

4 Maintenance Procedures .......................................................................................... 89

Maintenance schedule .......................................................................................................... 91

Spare parts ............................................................................................................................ 93

Troubleshooting with Connections INSIGHT ................................................................... 94

Safety and handling ............................................................................................................. 95

Preparing the instrument for operations on or inside its source ..................................... 97

Removing and refitting the source enclosure .................................................................... 98

Removing the source enclosure from the instrument....................................................... 98

Fitting the source enclosure to the instrument................................................................ 100

Installing and removing the corona pin ............................................ .... ..... ...................... 101

Installing the corona pin in the source............................................................................ 101

Removing the corona pin from the source...................................................................... 103

Operating the source isolation valve ................................................................................ 104

Removing O-rings and seals .............................................................................................. 107

Cleaning the instrument case ............................................................................................ 108

Emptying the exhaust trap bottle ..................................................................................... 108

Gas ballasting the roughing pump .................................................................................... 110

Checking the roughing pump oil level ............................................................................... 112

Adding oil to the roughing pump ....................................................................................... 113

Cleaning the source components ......................................... ..... .... ..................................... 114

Cleaning the sampling cone assembly ............................................................................... 115

Removing the sampling cone assembly from the source................................................. 115

Disassembling the sampling cone assembly.................................................................... 117

Cleaning the sample cone and cone gas nozzle.............................................................. 120

Assembling the sampling cone assembly....................................................................... 122

Fitting the sampling cone assembly to the source.......................................................... 123

Cleaning the ion block assembly ....................................................................................... 125

Removing the ion block assembly from the source assembly........................................ 125

xviii January 11, 2016, 715004599 Rev. B

Disassembling the source ion block assembly................................................................ 127

Cleaning the ion block components................................................................................ 132

Assembling the source ion block assembly.................................................................... 133

Fitting the ion block assembly to the source assembly................................................... 135

Cleaning the ion guide assembly ....................................................................................... 135

Removing the pumping block assembly and ion guide assembly from the instrument . 136
Removing the ion guide assembly and differential aperture from the pumping block

assembly................................................................................................................... 137

Removing the differential aperture support and the differential aperture from the ion guide

assembly................................................................................................................... 138

Cleaning the differential aperture................................................................................... 140

Cleaning the ion guide assembly.................................................................................... 141

Fitting the differential aperture and the differential aperture support onto the ion guide

assembly................................................................................................................... 143

Fitting the ion guide assembly and differential aperture onto the pumping block assembly.

144

Fitting the pumping block assembly and ion guide assembly onto the instrument........ 145

Replacing the ESI probe tip and gasket ........................................................................... 146

Replacing the probe tip and gasket................................................................................. 146

Replacing the ESI probe capillary.................................................................................. 150

Cleaning the IonSABRE II probe tip ............................................................................... 166

Replacing the IonSABRE II probe sample capillary ...................................................... 167

Removing the existing capillary..................................................................................... 167

Installing the new capillary............................................................................................. 169

Cleaning or replacing the corona pin ............................................................................... 172

Replacing the IonSABRE II probe heater ....................................................................... 174

Removing the IonSABRE II probe heater...................................................................... 174

Fitting the new IonSABRE II probe heater .................................................................... 176

Replacing the ion block source heater .............................................................................. 177

Replacing the source assembly seals ................................................................................. 180

Removing the probe adjuster assembly probe and source enclosure seals..................... 181

Fitting the new source enclosure and probe adjuster assembly seals............................. 183

January 11, 2016, 715004599 Rev. B xix

Replacing the air filter ....................................................................................................... 184

Replacing the roughing pump oil ..................................................................................... 187

Replacing the roughing pump’s oil demister element .................................................... 190

APPI/APCI source—changing the UV lamp bulb .......................................................... 195

APPI/APCI source—cleaning the lamp window ............................................................. 196

APPI/APCI source—replacing the APPI lamp drive seals ............................................ 198

Removing the APPI lamp drive assembly seals............................................................. 198

Fitting the new APPI lamp drive assembly O-rings..................................... ..... .... ......... 204

Replacing the fluidic lines of the ionKey source .............................................................. 206

Removing a fluidic line .............................................................. ..... ............................... 207

Installing a fluidic line.................................................................................................... 210

Cleaning the ionKey source and connectors ..................................................................... 211

Replacing the instrument’s fuses ...................................................................................... 214

A Safety Advisories .................................................................................................... 215

Warning symbols ................................................................................................................ 216

Specific warnings............................................................................................................ 217

Notices ................................................................................................................................. 220

Prohibition symbol ............................................................................................................. 220

Warnings that apply to all Waters instruments and devices .......................................... 221

Warnings that address the replacing of fuses .................................................................. 226

Electrical and handling symbols ....................................................................................... 227

Electrical symbols........................................................................................................... 227

Handling symbols........................................................................................................... 228

B External Connections ............................................................................................. 231

External wiring and vacuum connections ........................................................................ 232

Connecting the oil-filled roughing pump ......................................................................... 233

Connecting electric cables to the oil-filled roughing pump............................................ 237

xx Janua ry 11, 2016, 715004599 Rev. B

Connecting the Edwards oil-free roughing pump ........................................................... 238

Connecting electric cables to the Edwards oil-free roughing pump............................... 241

Connecting to the nitrogen gas supply ............................................................................. 242

Connecting to the collision cell gas supply ....................................................................... 243

Connecting the nitrogen exhaust line ............................................................................... 244

Connecting the liquid waste line ....................................................................................... 246

Connecting the workstation .............................................................................................. 249

Connecting Ethernet cables .............................................................................................. 250

I/O signal connectors ......................................................................................................... 251

Signal connections.......................................................................................................... 252

Connecting to the power supply ....................................................................................... 255

C Materials of Construction and Compliant Solvents ........................................... 257

Preventing contamination ................................................................................................. 258

Items exposed to solvent .................................................................................................... 258

Solvents used to prepare mobile phases ........................................................................... 259

D Plumbing the IntelliStart Fluidics System ........................................................... 261

Preventing contamination ................................................................................................. 262

The selector valve ............................................................................................................... 262

Plumbing schematic ........................................................................................................... 263

Tubing and connection specifications ............................................................................... 264

January 11, 2016, 715004599 Rev. B xxi

xxii January 11, 2016, 715004599 Rev. B

1 Specifications and Operating

Modes

This chapter describes the instrument, including its controls and connections for gas
and plumbing.

Contents:
Topic Page

Uses and compatibility ................................................................................. 24

Ionization techniques and source probes...................................................... 31

IntelliStart Fluidics system........................................................................... 34

Ion optics ...................................................................................................... 36

MS operating modes..................................................................................... 36

MS/MS operating modes.............................................................................. 38

Sample inlet.................................................................................................. 43

Leak sensors ................................................................................................. 43

Vacuum system............................................................................................. 43

Rear panel connections................................................................................. 44

January 11, 2016, 715004599 Rev. B 23

1 Specifications and Operating Modes

Uses and compatibility

The Waters® Xevo® TQ-S micro is a triple quadrupole, atmospheric pressure ionization
(API) mass spectrometer. Designed for routine HPLC/MS/MS and UPLC

analyses in quantitative and qualitative applications, it can operate at fast acquisition
speeds compatible with UltraPerformance LC

You can use the TQ-S micro with the following high-performance Waters ZSpray™
sources:

• Standard multimode electrospray ionization/atmospheric pressure chemical
ionization/combined electrospray ionization and atmospheric pressure chemical

ionization (ESI/APCI/ESCi

Requirement: Dedicated APCI operation requires an additional probe

(IonSABRE™ II).

• Optional dual-mode APPI/APCI source

• Optional NanoFlow™ ESI source

• Optional ionKey

®

source

®

) source.

®

applications.

®

/MS/MS

• Optional APGC source

• Optional ASAP probe

For information on installing and removing the optional APGC, and ASAP probe, refer to
the operator’s guide supplements supplied with them.

You can also use the Xevo TQ-S micro with the following optional third-party sources:

• DART

®

• DESI

• LDTD™

For further details, refer to the appropriate manufacturer’s documentatio n.

Note: Available source options can vary depending on the software used to operate the

Xevo TQ-S micro. Refer to the MassLynx

®

online Help for more information about

supported sources.
For mass spectrometer specifications, see the Waters Xevo TQ-S micro Site Preparation

Guide.

24 Janua ry 11 , 20 16 , 71 50 0 459 9 R ev . B

Xevo TQ-S micro shown with visor down, and visor up:

TP03407

Visor up

Uses and compatibility

IntelliStart technology

IntelliStart™ technology monitors LC/MS/MS performance and reports when the
instrument is ready for use.

The software automatically tunes and mass calibrates the instrument, displays
performance readbacks, and enables simplified setup of the system for use in routine
analytical and open access applications. (See page 29.)

January 11, 2016, 715004599 Rev. B 25

1 Specifications and Operating Modes

The IntelliStart Fluidics1 system is built into the instrument. It delivers sample directly to
the MS probe from the LC column or from two integral reservoirs. The integral reservoirs
can also deliver sample through direct or combined infusion so that you can optimize
instrument performance at analytical flow rates. See the instrument’s online Help for
further details of IntelliStart.

ACQUITY Xevo TQ-S micro UPLC/MS systems

The Waters Xevo TQ-S micro is compatible with the following ACQUITY UPLC®
systems:

• ACQUITY UPLC

• ACQUITY UPLC H-Class

• ACQUITY UPLC I-Class

• ACQUITY UPLC M-Class (with NanoFlow or ionKey source)

If you are not using one of these systems, refer to the documentation relevant to your LC
system.

The ACQUITY
ACQUITY UPLC H-Class, or ACQUITY UPLC I-Class and the Waters Xevo TQ-S
micro fitted with the ESI/APCI/ESCi source.

The ACQUITY
M-Class system and the W aters Xevo TQ-S micro fitted with either a Nanoflow source, or
an ionKey source.

®

Xevo TQ-S micro UPLC/MS system includes an ACQUITY UPLC,

®

Xevo TQ-S micro UPLC/MS system can include an ACQUITY UPLC

If you are not using your instrument as part of an ACQUITY UPLC system, refer to the
documentation for your LC system.

Note: ACQUITY system options can vary depending on the software used to operate the

Xevo TQ-S micro.

1. In Waters product documentation, the term “fluidics” denotes plumbing connections
and components and the fluid pathways within and among instruments or devices. It also
appears in the product name “IntelliStart™ Fluidics” where it describes a mass
spectrometer’s integral apparatus for delivering sample and solvent directly to the
instrument’s probe. Finally, the term can arise in the context of a component part name, as
in “fluidics drawer”.

26 Janua ry 11 , 20 16 , 71 50 0 459 9 R ev . B

Uses and compatibility

ACQUITY system core components

Core system components for each ACQUITY system are listed below:

System Core components

ACQUITY UPLC • Binary solvent manager

• Sample manager

• Column heater

•UPLC detectors

• Solvent tray

• ACQUITY UPLC column

• Software to control the system

ACQUITY UPLC H-Class • Quaternary solvent manager

• Sample manager with flow-through needle

• Column heater-active

•UPLC detectors

• Solvent tray

• ACQUITY UPLC column

• Software to control the system

ACQUITY UPLC I-Class • Binary solvent manager

• Sample manager

• Column heater-active

•UPLC detectors

• Solvent tray

• ACQUITY UPLC column

• Software to control the system

January 11, 2016, 715004599 Rev. B 27

1 Specifications and Operating Modes

System Core components

ACQUITY UPLC M-Class • µBinary solvent manager

For further instruction, see the ACQUITY UPLC System Operator’s Guide, ACQUITY

UPLC H-Class System Guide, ACQUITY UPLC I-Class System Guide, and Controlling
Contamination in UPLC/MS and HPLC/MS Systems (part number 715001307). You can

find the documents on http://www.waters.com; click Services and Support > Support
Library.

• µSample manager - FL

• Trap Valve Manager (including active column
heater)

• TuV and PDA UPLC detectors

• Solvent tray

• ACQUITY UPLC columns of internal diameters
ranging from 75 µm to 1 mm. The column
hardware and the matched outlet tubing can
withstand pressure of as much as 15,000 psi.

• Software to control the system

28 Janua ry 11 , 20 16 , 71 50 0 459 9 R ev . B

Xevo TQ-S micro with ACQUITY UPLC system:

Sample organizer (optional)

Solvent tray

Column heater

Xevo TQ-S micro

Sample manager

Binary solvent
manager

Uses and compatibility

Software and data system

MassLynx v4.1 software can control the mass spectrometer. See page 30 for more
information about those applications.

MassLynx software enables these major operations:

• Configuring the system

• Creating LC and MS/MS methods that define operating parameters for a run

• Using IntelliStart software to automatically tune and mass calibrate the mass

• Running samples

• Monitoring the run

• Acquiring data

• Processing data

• Reviewing data

• Printing data

spectrometer

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1 Specifications and Operating Modes

MassLynx v4.1

MassLynx software acquires, analyzes, manages, and distributes mass spectrometry,
ultraviolet (UV), evaporative light scattering, and analog data. OpenLynx

TM

TargetLynx
See the MassLynx v4.1 user documentation and online Help for information about using

MassLynx software.
You configure settings, monitor performance, run diagnostic tests, and maintain the

system and its modules via the MassLynx Instrument Console application.
The Instrument Console software, which functions independently of MassLynx software,

does not recognize or control data systems.
See the online Help for the Instrument Console system for details.

application managers are included as standard software with MassLynx.

TM

and

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