Waters Xevo G2-XS QTof Overview And Maintenance Manual

Waters Xevo G2-XS QTof

Overview and Maintenance Guide

715004496/Revision B

Copyright © Waters Corporation 2015

All rights reserved

ii April 2, 2015, 715004496 Rev. B

General Information

Copyright notice

© 2014 – 2015 WATERS CORPORATION. PRINTED IN THE UNITED
STATES OF AMERICA AND IN IRELAND. ALL RIGHTS RESERVED. THIS
DOCUMENT OR PARTS THEREOF MAY NOT BE REPRODUCED IN ANY
FORM WITHOUT THE WRITTEN PERMISSION OF THE PUBLISHER.

The information in this document is subject to change without notice and
should not be construed as a commitment by Waters Corporation. Waters
Corporation assumes no responsibility for any errors that may appear in this
document. This document is believed to be complete and accurate at the time
of publication. In no event shall Waters Corporation be liable for incidental or
consequential damages in connection with, or arising from, its use. For the
most recent revision of this document, consult the Waters Web site
(waters.com).

Trademarks

Waters, “THE SCIENCE OF WHAT’S POSSIBLE.”, ACQUITY,
ACQUITY UPC

UNIFI, UPLC, Waters Quality Parts, and Xevo are registered trademarks of
Waters Corporation, and iKey, IntelliStart, ionKey, ionKey/MS, IonSABRE,
LockSpray, NanoFlow, NanoLockSpray, OpenLynx, pDRE, StepWave,
TargetLynx, and ZSpray are trademarks of Waters Corporation.

2

, ACQUITY UPLC, Connections INSIGHT, ESCi, MassLynx,

LEYBONOL is a registered trademark of Oerlikon Leybold Vacuum GmbH.
POZIDRIV is a registered trademark of Phillips Screw Company, Inc.
Swagelok is a registered trademark of Swagelok Company.
Tygon is a registered trademark of Saint-Gobain Performance Plastics Corp.
Viton is a registered trademark of E. I. du Pont de Nemours and Company.
Windows is a registered trademark of Microsoft Corporation in the United

States and/or other countries.
Xylan is a registered trademark of Whitford Corporation.
PEEK is a trademark of Victrex plc.
Super Flangeless and SealTight are trademarks of Upchurch Scientific, Inc.

April 2, 2015, 715004496 Rev. B iii

TaperTip is a trademark of New Objective, Inc.
Valco is a trademark of Valco Instruments, Inc.
Other registered trademarks or trademarks are the sole property of their

owners.

Customer comments

Waters’ Technical Communications organization invites you to report any
errors that you encounter in this document or to suggest ideas for otherwise
improving it. Help us better understand what you expect from our
documentation so that we can continuously improve its accuracy and
usability.

We seriously consider every customer comment we receive. You can reach us
at [e-mail].

Contacting Waters

Contact Waters with enhancement requests or technical questions regarding
the use, transportation, removal, or disposal of any Waters product. You can
reach us via the Internet, telephone, or conventional mail.

Waters contact information:

Contacting medium Information

Internet The Waters Web site includes contact

Telephone and fax From the USA or Canada, phone

Conventional mail Waters Corporation

iv April 2, 2015, 715004496 Rev. B

information for Waters locations worldwide.
Visit www.waters.com.

800 252-4752, or fax [phone].
For other locations worldwide, phone and fax

numbers appear in the Waters Web site.

34 Maple Street
Milford, MA 01757
USA

Safety considerations

Some reagents and samples used with Waters instruments and devices can
pose chemical, biological, or radiological hazards (or any combination thereof).
You must know the potentially hazardous effects of all substances you work
with. Always follow Good Laboratory Practice, and consult your organization’s
standard operating procedures.

Safety hazard symbol notice

Documentation needs to be consulted in all cases where the symbol is
used to find out the nature of the potential hazard and any actions which have
to be taken.

Considerations specific to the Xevo G2-XS QTof

Solvent leakage hazard

The source exhaust system is designed to be robust and leak-tight. Waters
recommends you perform a hazard analysis assuming a maximum leak into
the laboratory atmosphere of 10% LC eluate.

Warning:

• To confirm the integrity of the source exhaust system, renew
the source O-rings at intervals not exceeding one year.

• To avoid chemical degradation of the source O-rings, which can
withstand exposure only to certain solvents (see page 305),
determine whether any solvents you use that are not listed are
chemically compatible with the composition of the O-rings.

April 2, 2015, 715004496 Rev. B v

Spilled solvents hazard

Prohibited: To avoid injury or equipment damage caused by spilled

solvent, do not place reservoir bottles on top of the instrument or on its
front ledge, unless in the bottle tray provided.

Flammable solvents hazard

Warning: To prevent ignition of flammable solvent vapors in the

enclosed space of a mass spectrometer’s ion source, ensure that nitrogen
flows continuously through the source. The nitrogen supply pressure
must not fall below 400 kPa (4.0 bar, 60 psi) during an analysis
requiring the use of flammable solvents. Also a gas-fail device must be
installed, to interrupt the flow of LC solvent should the nitrogen supply
fail.

When using flammable solvents, ensure that a stream of nitrogen
continuously flushes the instrument’s source, and the nitrogen supply
pressure remains above 400 kPa (4.0 bar, 60 psi). You must also install a
gas-fail device that interrupts the solvent flowing from the LC system in the
event the supply of nitrogen fails.

vi April 2, 2015, 715004496 Rev. B

High temperature hazard

Source ion block assembly

Warning: The source ion block, located behind the source enclosure

assembly, can become hot. To avoid burn injuries, ensure the source
heater is turned off and the ion block is cool before performing
maintenance on these components.

Mass spectrometer high temperature hazard:

April 2, 2015, 715004496 Rev. B vii

High voltage hazard

Warning:

• To avoid electric shock, do not remove the mass spectrometer’s
protective panels. The components they cover are not
user-serviceable.

• To avoid nonlethal electric shock when the instrument is in Operate
mode, avoid touching the areas marked with the high voltage
warning symbol. To touch those areas, first put the instrument in
Standby mode.

Hazards associated with removing an instrument from service

Warning: To avoid personal contamination with biohazards,

toxic materials, and corrosive materials, wear
chemical-resistant gloves during all phases of instrument
decontamination.

Warning: To avoid puncture injuries, handle syringes, fused silica lines,

and borosilicate tips with extreme care.

When you remove the instrument from use to repair or dispose of it, you must
decontaminate all of its vacuum areas. These are the areas in which you can
expect to encounter the highest levels of contamination:

• Source interior

• Waste tubing

• Exhaust system

• Rotary pump oil (where applicable)

The need to decontaminate other vacuum areas of the instrument depends on
the kinds of samples the instrument analyzed and their levels of
concentration. Do not dispose of the instrument or return it to Waters for
repair until the authority responsible for approving its removal from the
premises specifies the extent of decontamination required and the level of
residual contamination permissible. That authority must also prescribe the
method of decontamination to be used and the appropriate protection for
personnel undertaking the decontamination process.

You must handle items such as syringes, fused silica lines, and borosilicate
tips used to carry sample into the source area in accordance with laboratory

viii April 2, 2015, 715004496 Rev. B

procedures for contaminated vessels and sharps. To avoid contamination by
carcinogens, toxic substances, or biohazards, you must wear
chemical-resistant gloves when handling or disposing of used oil.

Bottle placement prohibition

Prohibited: To avoid injury from electric shock or fire, and to prevent

damage to the workstation and ancillary equipment, do not place objects
filled with liquid—such as solvent bottles—on these items, or expose
them to dripping or splashing liquids.

Notice: To prevent spillages, do not lift the bottle tray while it is full of

bottles.

FCC radiation emissions notice

Changes or modifications not expressly approved by the party responsible for
compliance, could void the users authority to operate the equipment. This
device complies with Part 15 of the FCC Rules. Operation is subject to the
following two conditions: (1) this device may not cause harmful interference,
and (2) this device must accept any interference received, including
interference that may cause undesired operation.

Electrical power safety notice

Position the instrument and ancillary equipment in positions where it is easy
to reach and disconnect the power cable from the instrument’s rear panel.

Safety advisories

Consult Appendix A for a comprehensive list of warning advisories and
notices.

April 2, 2015, 715004496 Rev. B ix

Operating the Xevo G2-XS QTof

When operating the Xevo® G2-XS QTof, follow standard quality-control (QC)
procedures and the guidelines presented in this section.

Applicable symbols

Symbol Definition

Manufacturer

Date of manufacture

Authorized representative of the European
Community

Confirms that a manufactured product complies
with all applicable European Community
directives

Australia EMC compliant

or

x April 2, 2015, 715004496 Rev. B

Confirms that a manufactured product complies
with all applicable United States and Canadian
safety requirements

Consult instructions for use

Alternating current

Symbol Definition

5()

Audience and purpose

This guide is intended for operators of varying levels of experience. It provides
an overview of the instrument, and explains how to prepare it, change its
modes of operation, and maintain it.

Electrical and electronic equipment with this
symbol may contain hazardous substances and
should not be disposed of as general waste.

For compliance with the Waste Electrical and
Electronic Equipment Directive (WEEE)
2012/19/EU, contact Waters Corporation for the
correct disposal and recycling instructions.

Serial number

Part number catalog number

Intended use of the Xevo G2-XS QTof

Waters designed the orthogonal acceleration, time-of-flight Xevo G2-XS QTof
for use as a research tool to deliver authenticated mass measurement. The
Xevo G2-XS QTof is for research use only and is not intended for use in
diagnostic applications.

Calibrating

To calibrate LC systems, follow acceptable calibration methods using at least
five standards to generate a standard curve. The concentration range for
standards should cover the entire range of QC samples, typical specimens,
and atypical specimens.

To calibrate the Xevo G2-XS QTof, consult the instrument’s online Help
system.

April 2, 2015, 715004496 Rev. B xi

Quality control

Routinely run three QC samples that represent subnormal, normal, and
above-normal levels of a compound. If sample trays are the same or very
similar, vary the location of the QC samples in the trays. Ensure that QC
sample results fall within an acceptable range, and evaluate precision from
day to day and run to run. Data collected when QC samples are out of range
might not be valid. Do not report these data until you are certain that the
instrument performs satisfactorily.

When analyzing samples from a complex matrix such as soil, tissue,
serum/plasma, whole blood, and other sources, note that the matrix
components can adversely affect LC/MS results, enhancing or suppressing
ionization. To minimize these matrix effects, adopt the following measures:

• Prior to the instrumental analysis, use appropriate sample
pretreatment such as protein precipitation, liquid/liquid extraction
(LLE), or solid phase extraction (SPE) to remove matrix interferences.

• Whenever possible, verify method accuracy and precision using
matrix-matched calibrators and QC samples.

• Use one or more internal standard compounds, preferably isotopically
labeled analytes.

Equipment misuse notice

If the equipment is used in a manner not specified by the manufacturer, the
protection provided by the equipment may be impaired.

xii April 2, 2015, 715004496 Rev. B

EMC considerations

Canada spectrum management emissions notice

This class A digital product apparatus complies with Canadian ICES-001.
Cet appareil numérique de la classe A est conforme à la norme NMB-001.

ISM Classification: ISM Group 1 Class A

This classification has been assigned in accordance with CISPR 11 Industrial
Scientific and Medical, (ISM) instrument requirements. Group 1 products
apply to intentionally generated and/or used conductively coupled
radio-frequency energy that is necessary for the internal functioning of the
equipment. Class A products are suitable for use in all establishments other
than residential locations and those directly connected to a low voltage power
supply network supplying a building for domestic purposes.

There may be potential difficulties in ensuring electromagnetic compatibility
in other environments due to conducted as well as radiated disturbances.

Do not use the equipment in close proximity to sources of strong
electromagnetic radiation (for example, unshielded intentional RF sources), as
these may interfere with the equipment’s proper operation.

This equipment complies with the emission and immunity requirements
described in the relevant parts of IEC/EN 61326: Electrical equipment for

measurement, control and laboratory use — EMC requirements.

April 2, 2015, 715004496 Rev. B xiii

EC authorized representative

Waters Corporation
Stamford Avenue
Altrincham Road
Wilmslow SK9 4AX UK

Telephone: [phone]
Fax: [phone]
Contact: Quality manager

xiv April 2, 2015, 715004496 Rev. B

Table of Contents

General Information .................................................................................... iii

Copyright notice .................................................................................................. iii

Trademarks ........................................................................................................... iii

Customer comments ............................................................................................ iv

Contacting Waters ............................................................................................... iv

Safety considerations ........................................................................................... v

Safety hazard symbol notice................................................................................ v

Considerations specific to the Xevo G2-XS QTof................................................ v

FCC radiation emissions notice ......................................................................... ix

Electrical power safety notice ............................................................................ ix

Safety advisories................................................................................................. ix

Operating the Xevo G2-XS QTof ........................................................................ x

Applicable symbols .............................................................................................. x

Audience and purpose......................................................................................... xi

Intended use of the Xevo G2-XS QTof ............................................................... xi

Calibrating .......................................................................................................... xi

Quality control .................................................................................................. xii

Equipment misuse notice ................................................................................. xii

EMC considerations ......................................................................................... xiii

Canada spectrum management emissions notice .......................................... xiii

ISM Classification: ISM Group 1 Class A ...................................................... xiii

EC authorized representative ......................................................................... xiv

1 Waters Xevo G2-XS QTof Overview ..................................................... 23

Waters Xevo G2-XS QTof .................................................................................. 24

IntelliStart technology....................................................................................... 25

ACQUITY Xevo G2-XS QTof UPLC/MS systems............................................. 26

Software and data system ................................................................................. 29

April 2, 2015, 715004496 Rev. B xv

LockSpray source and ionization modes ..................................................... 31

Electrospray ionization (ESI)............................................................................ 32

Atmospheric pressure chemical ionization (APCI) .......................................... 32

Combined electrospray/atmospheric pressure chemical ionization (ESCi) .... 33

Atmospheric solids analysis probe (ASAP)....................................................... 33

NanoLockSpray source and ionization modes ........................................... 34

Combined APPI/APCI source .......................................................................... 36

Atmospheric pressure gas chromotography (APGC) source ................... 36

ionKey source ..................................................................................................... 37

IntelliStart Fluidics system ............................................................................. 38

IntelliStart Fluidics system physical layout .................................................... 39

System operation ............................................................................................... 40

Ion optics .............................................................................................................. 41

Leak sensors ........................................................................................................ 43

Vacuum system ................................................................................................... 43

2 Preparing the Mass Spectrometer for Operation ............................ 45

Starting the mass spectrometer ..................................................................... 46

Verifying the instrument’s state of readiness .................................................. 48

Monitoring the mass spectrometer LEDs......................................................... 48

Calibration ......................................................................................................... 48

Flow rates for the Xevo G2-XS QTof system .................................................... 49

Preparing the IntelliStart Fluidics system .................................................. 49

Installing the reservoir bottles.......................................................................... 49

Adjusting the solvent delivery tube positions .................................................. 52

Purging the pump .............................................................................................. 53

xvi April 2, 2015, 715004496 Rev. B

Rebooting the mass spectrometer .................................................................. 53

Leaving the mass spectrometer ready for operation ................................ 54

Emergency shutdown of the mass spectrometer ........................................ 54

3 Configuring the LockSpray Source .................................................... 55

Configuring the LockSpray source ................................................................ 56

Configuring for ESI mode ................................................................................ 57

Installing the ESI probe .................................................................................... 57

Removing the ESI probe.................................................................................... 61

Configuring for APCI mode ............................................................................. 62

Installing the IonSABRE II probe .................................................................... 62

Removing the IonSABRE II probe .................................................................... 65

Configuring for ESCi mode ............................................................................. 66

Optimizing the ESI probe for ESCi operation.................................................. 66

4 Configuring the NanoLockSpray Source .......................................... 67

Overview of the NanoLockSpray source ...................................................... 68

Sample sprayer .................................................................................................. 69

LockSpray sprayer............................................................................................. 69

NanoFlow gas supply......................................................................................... 70

Purge gas............................................................................................................ 70

Sprayer platform adjuster assembly................................................................. 70

April 2, 2015, 715004496 Rev. B xvii

Selecting and configuring the NanoLockSpray source ............................ 71

Deploying the sprayer platform adjuster assembly .................................. 72

Adjusting the sprayer tip position ................................................................. 73

Setting up the camera ....................................................................................... 74

Optional glass-capillary sprayer .................................................................... 75

5 Installing and removing the ionKey source ..................................... 77

Installing the ionKey source ........................................................................... 78

Installing ionKey source software ................................................................. 87

Installing the camera in the ionKey source ................................................ 87

Removing the ionKey source .......................................................................... 88

6 Maintenance Procedures ...................................................................... 91

Maintenance schedule ...................................................................................... 93

Spare parts .......................................................................................................... 95

Troubleshooting with Connections INSIGHT ............................................. 96

Safety and handling .......................................................................................... 97

Preparing the instrument for working on the source .............................. 99

Removing and refitting the source enclosure ........................................... 100

Removing the source enclosure from the instrument.................................... 100

Fitting the source enclosure to the instrument.............................................. 102

Installing and removing the corona pin ..................................................... 103

Installing the corona pin in the source........................................................... 103

Removing the corona pin from the source ...................................................... 106

xviii April 2, 2015, 715004496 Rev. B

Operating the source isolation valve .......................................................... 107

Removing O-rings and seals .......................................................................... 110

Cleaning the instrument case ....................................................................... 111

Emptying the nitrogen exhaust-trap bottle ............................................... 112

Maintaining the roughing pump .................................................................. 115

Maintaining the Oerlikon Leybold oil-filled roughing pump ................ 115

Gas ballasting the Oerlikon Leybold roughing pump.................................... 116

Inspecting the roughing pump oil level .......................................................... 117

Adding oil to the roughing pump .................................................................... 117

Replacing the roughing pump’s oil and oil demister elements...................... 120

Cleaning the source components ................................................................. 127

Cleaning the sampling cone assembly ........................................................ 128

Removing the sampling cone assembly from the source ............................... 128

Disassembling the sampling cone assembly................................................... 130

Cleaning the sample cone and cone gas nozzle .............................................. 133

Assembling the sampling cone assembly........................................................ 135

Fitting the sampling cone assembly to the source ......................................... 136

Cleaning the ion block assembly .................................................................. 138

Removing the ion block assembly from the source assembly........................ 138

Disassembling the source ion block assembly................................................ 141

Cleaning the ion block components ................................................................ 147

Assembling the source ion block assembly..................................................... 149

Fitting the ion block assembly to the source assembly.................................. 151

Cleaning the StepWave ion guide assembly .............................................. 152

Handling the StepWave ion guide assembly.................................................. 152

Removing the ion block support from the source assembly........................... 152

Removing the StepWave assembly from the source assembly...................... 154

Disassembling the StepWave ion guide assembly ......................................... 158

Cleaning the StepWave ion guide assembly................................................... 161

Assembling the StepWave ion guide assembly .............................................. 165

Fitting the StepWave assembly to the source assembly................................ 167

Fitting the ion block support to the source..................................................... 170

April 2, 2015, 715004496 Rev. B xix

Replacing the ESI probe tip and gasket ..................................................... 171

Removing the ESI probe tip and gasket ......................................................... 171

Fitting the ESI probe tip and gasket.............................................................. 174

Replacing the ESI probe sample capillary ................................................ 175

Removing the existing capillary...................................................................... 175

Installing the new capillary ............................................................................ 180

Cleaning the IonSABRE II probe tip ........................................................... 184

Replacing the IonSABRE II probe sample capillary ............................... 184

Removing the existing capillary...................................................................... 184

Installing the new capillary ............................................................................ 187

Replacing the LockSpray reference probe capillary .............................. 191

Removing the existing capillary...................................................................... 191

Installing the new capillary ............................................................................ 194

Replacing the NanoLockSpray reference-probe TaperTip emitter or

capillary ...................................................................................................... 197

Removing the NanoLockSpray reference probe ............................................. 197

Installing the new TaperTip emitter and capillary ....................................... 199

Replacing the ionKey reference-probe capillary ..................................... 202

Removing the ionKey reference probe ............................................................ 202

Installing the new TaperTip emitter and capillary ....................................... 204

Cleaning or replacing the corona pin ......................................................... 207

Replacing the IonSABRE II probe heater .................................................. 208

Removing the IonSABRE II probe heater ...................................................... 208

Fitting the new IonSABRE II probe heater.................................................... 210

Replacing the ion block source heater ....................................................... 211

Replacing the LockSpray source’s assembly seals .................................. 215

Removing the probe adjuster assembly probe and source

enclosure seals ........................................................................................... 215

Fitting the new source enclosure and probe adjuster assembly

probe seals.................................................................................................. 217

xx April 2, 2015, 715004496 Rev. B

Replacing the mass spectrometer’s air filters ........................................... 219

Replacing the air filter inside the front door.................................................. 219

Replacing the air filters on the sides of the instrument................................ 221

Replacing the IntelliStart Fluidics tubing ................................................ 223

Replacing IntelliStart Fluidics tubing (Standard configuration) ........ 224

Removing the IntelliStart Fluidics tubing ..................................................... 225

Plumbing the IntelliStart Fluidics LockSpray system .................................. 226

Plumbing the IntelliStart Fluidics sample delivery system.......................... 235

Plumbing the sample fluidics delivery system for NanoLockSpray

operation ..................................................................................................... 241

Replacing the fluid lines of the ionKey source ......................................... 249

Removing a fluid line....................................................................................... 250

Installing a fluid line ....................................................................................... 252

Cleaning the ionKey source and connectors ............................................. 254

A Safety Advisories .................................................................................. 257

Warning symbols .............................................................................................. 258

Specific warnings ............................................................................................. 259

Notices ................................................................................................................ 262

Prohibition symbol .......................................................................................... 262

Warnings that apply to all Waters instruments and devices ................. 263

Warnings that address the replacing of fuses ........................................... 268

Electrical and handling symbols .................................................................. 270

Electrical symbols ............................................................................................ 270

Handling symbols ............................................................................................ 271

B External Connections .......................................................................... 273

Mass spectrometer: external wiring and vacuum connections ............ 274

Connecting the Oerlikon Leybold oil-filled roughing pump ................. 275

Making the electrical connections to the Oerlikon Leybold oil-filled roughing

pump........................................................................................................... 279

April 2, 2015, 715004496 Rev. B xxi

Connecting the Edwards oil-free roughing pump ................................... 280

Making the electrical connections to the Edwards oil-free roughing pump . 283

Connecting to the nitrogen gas supply ....................................................... 284

Connecting to the collision cell gas supply ............................................... 285

Connecting the nitrogen exhaust line ........................................................ 286

Connecting liquid waste lines ....................................................................... 288

Connecting the workstation (systems with no ACQUITY LC) .............. 291

Connecting Ethernet cables (systems with ACQUITY LC) .................... 292

Input/output signal connectors .................................................................... 292

Signal connections ........................................................................................... 295

Connecting the contact-closure cable to an ACQUITY LC ..................... 298

Connecting to the electricity source ........................................................... 300

Connecting the camera for the NanoLockSpray source or

ionKey source ............................................................................................ 301

Installing the camera driver software ............................................................ 301

C Materials of Construction and Compatible Solvents ................... 303

Preventing contamination ............................................................................. 303

Items exposed to solvent ................................................................................ 304

Solvents used to prepare mobile phases .................................................... 305

xxii April 2, 2015, 715004496 Rev. B

1 Waters Xevo G2-XS QTof

Overview

This chapter describes the instrument, including its controls, sources,
and IntelliStart™ Fluidics system.

Contents:

Topic Page

Waters Xevo G2-XS QTof................................................................. 24

LockSpray source and ionization modes......................................... 31

NanoLockSpray source and ionization modes................................ 34

Combined APPI/APCI source .......................................................... 36

Atmospheric pressure gas chromotography (APGC) source.......... 36

ionKey source ................................................................................... 37

IntelliStart Fluidics system............................................................. 38

Ion optics .......................................................................................... 41

Leak sensors..................................................................................... 43

Vacuum system ................................................................................ 43

April 2, 2015, 715004496 Rev. B 23

1 Waters Xevo G2-XS QTof Overview

Waters Xevo G2-XS QTof

The Xevo® G2-XS QTof Mass Spectrometry (MS) system is a hybrid,
quadrupole, orthogonal acceleration, time-of-flight (Tof) mass spectrometer

operated by Waters
One of the following high-performance, ZSpray™, dual-orthogonal, API

sources is fitted as standard equipment:

•LockSpray™ (ESI/APCI/ESCi) source, which combines these ionization
modes:

–ESI (see page 32)
–APCI (see page 32)

–ESCi

• NanoLockSpray™ ESI source, (see page 34).

The following optional sources are compatible with the Xevo G2-XS QTof:

• Combined APPI/APCI source (see page 36, and the Waters APPI Source
Operator’s Guide Supplement, part number 71500137602).

®

informatics software.

®

(combines ESI and APCI) (see page 33)

• Atmospheric Solids Analysis Probe (ASAP) (see page 33, and the
Atmospheric Solids Analysis Probe Operator’s Guide Supplement, part
number 715002034).

• Atmospheric Pressure Gas Chromatography (APGC) source (see

page 36, and the Atmospheric Pressure GC Operator’s Guide

Supplement, part number 715001804).

• ionKey™ source (see page 37, and the ionKey/MS System Guide, part
number 715004028).

Note: Available source options can vary, depending on the software you use to

operate the Xevo G2-XS QTof. Refer to the MassLynx

®

or UNIFI® online Help

for more information about supported sources.
For instrument specifications, consult the Waters Xevo G2-XS QTof/Tof Site

Preparation Guide (part number 715003608).

24 April 2, 2015, 715004496 Rev. B

IntelliStart technology

IntelliStart technology monitors instrument performance and reports when it
is ready for use.

The console software automatically mass calibrates the mass spectrometer
and displays performance readbacks to enable simplified setup of the system
for use in routine analytical and open access applications.

The IntelliStart Fluidics
delivers sample directly to the MS probe from the LC column or from three
integral reservoirs. The reservoirs can also deliver sample through direct or
combined infusion so that you can optimize instrument performance at
analytical flow rates. An additional reservoir contains solvent for the
automated flushing of the solvent delivery system.

Waters Xevo G2-XS QTof

1

system is built into the mass spectrometer. It

1. In Waters documents, the term “fluidics” refers to the IntelliStart Fluidics system, the
instrument’s onboard system that delivers sample and solvent to the probe of the mass
spectrometer. It can also denote plumbing components and fluid pathways within and between
system modules.

April 2, 2015, 715004496 Rev. B 25

1 Waters Xevo G2-XS QTof Overview

ACQUITY Xevo G2-XS QTof UPLC/MS systems

The Waters Xevo G2-XS QTof is compatible with the following systems:

• ACQUITY UPLC

• ACQUITY UPLC H-Class, and H-Class Bio

• ACQUITY UPLC I-Class

• ACQUITY UPLC M-Class

• ACQUITY UPC

If you do not use one of these systems, refer to the documentation specific to
your LC system.

The ACQUITY

®

Xevo G2-XS QTof UPLC®/MS system includes an ACQUITY
UPLC system, and the Waters Xevo G2-XS QTof fitted with either the
LockSpray ESI/APCI/ESCi source, or the NanoLockSpray source (with
ACQUITY UPLC M-Class only).

The ionKey/MS™ system includes an ACQUITY UPLC M-Class system, and
the Waters Xevo G2-XS QTof fitted with the ionKey source.

®

2®

ACQUITY UPLC system

The ACQUITY UPLC system includes a binary or quaternary solvent
manager, sample manager, column heater, sample organizer, detectors, and a
specialized ACQUITY UPLC column. Waters informatics software controls
the system.

For further information, see the relevant ACQUITY UPLC system operator’s
guide or system guide, and Controlling Contamination in UPLC/MS and
HPLC/MS Systems (part number 715001307). You can find the latter
document on http://www.waters.com; click Services & Support > Support.

26 April 2, 2015, 715004496 Rev. B

Waters ACQUITY Xevo G2-XS QTof UPLC/MS system:

Sample organizer (optional)

Solvent tray

Xevo G2-XS QTof

Sample manager

Binary solvent manager

Access door to the fluidics pumps

High voltage
connector for the
ESI probe

Probe

Source interface
sliding door

LockSpray source
enclosure

Access door to the
fluidics valves

Column heater

Waters Xevo G2-XS QTof

Note: An ACQUITY UPLC I-Class system is illustrated. See page 26 for

information about other ACQUITY UPLC systems supported by the
Xevo G2-XS QTof.

April 2, 2015, 715004496 Rev. B 27

1 Waters Xevo G2-XS QTof Overview

Solvent tray

Trap valve
manager

Xevo G2-XS QTof

µSample
manager

-fixed loop

Access door to the fluidics pumps

Access door to the fluidics valves

Source interface
sliding door

NanoLockSpray
source enclosure

µBinary
solvent
manager

ACQUITY UPLC M-Class system

The ACQUITY UPLC M-Class system includes a binary solvent manager,
auxiliary solvent manager, sample manager, column heater, sample
organizer, detectors, and a specialized ACQUITY UPLC M-Class column.
Waters informatics software controls the system.

For further information, see the ACQUITY UPLC M-Class System Guide (part
number 715003588) and Controlling Contamination in UPLC/MS and
HPLC/MS Systems (part number 715001307). You can find the latter
document on http://www.waters.com; click Services & Support > Support.

Waters ACQUITY M-Class Xevo G2-XS QTof UPLC/MS system:

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Software and data system

You can use MassLynx software or UNIFI software to control the mass
spectrometer.

Both MassLynx software and UNIFI software enable these major operations:

• Configuring the system

• Creating LC, MS, and MS/MS methods that define operating
parameters for a run

• Using IntelliStart software to automatically tune and mass calibrate the
mass spectrometer

• Running samples

• Monitoring the run

• Acquiring data

• Processing data

•Reviewing data

• Printing data

Waters Xevo G2-XS QTof

MassLynx software

MassLynx software acquires, analyzes, manages, and distributes mass
spectrometry, ultraviolet (UV), evaporative light scattering (ELS), and analog
data. OpenLynx™ and TargetLynx™ application managers are included with
MassLynx software.

See the MassLynx software user documentation and online Help for
information about using MassLynx software.

You configure settings, monitor performance, run diagnostic tests, and
maintain the system and its modules via the MassLynx Instrument Control
application.

The Instrument Control Software, which functions independently of
MassLynx software, does not recognize or control data systems.

See the online Help for the Instrument Console system for further details.

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1 Waters Xevo G2-XS QTof Overview

UNIFI software

UNIFI software integrates mass spectrometry, UPLC chromotography, and
informatics data workflows into one system.

See UNIFI software user documentation and online Help for more information
about using UNIFI software.

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