Waters Sentry Guard Column User Manual
[ Care and Use ManUal ]
Sentry Guard Columns
Integrated Guard
Column Holder
Universal Guard Column
sentrY GuArd CoLuMn
I. IntroduCtIon
Waters Sentry™ Guard Columns protect your analytical column or
cartridge column from particulate and chemical contamination in
t h e m o b i l e - p h a s e s t r e a m . C o n t a mi n a n t s t h a t m a y d a m ag e y o u r
analytical column are intercepted by the guard column.
Sentry Guard Columns and Guard Holders
Contents
I. IntroduCtIon
a. Packing Materials
II. InstALLInG tHe GuArd CoLuMn
a. Installing the Universal Guard Column Holder
b. Installing the Integrated Guard Column Holder
III. rePLACInG tHe GuArd CoLuMn
a. Procedure
IV. orderInG InForMAtIon
V. WArrAntY/serVICe InForMAtIon
VI. sPeCIFICAtIons
a. Packing Materials
The packing materials used in Waters Sentry guard columns are
identical to the packaging materials used in Waters HPLC columns.
Select guard columns with the same packing materials as your
analytical column.
Sentry Guard Column 1
[ Care and Use ManUal ]
Guard Column Holder
Plastic Bushing
Guard Column
Guard Column Holder
Guard Column Cartridge Column
Spacer
II. InstALLInG tHe GuArd CoLuMn
a. Installing the Universal Guard Column Holder
Install the Universal Guard Column Holder in your HPLC system
between the injector and the column. Use 0.009-inch (0.23 mm) i.d.
tubing for all connections.
III. rePLACInG tHe GuArd CoLuMn
R e p l a c e g u a r d c o l u m n s b e f o r e c o n t a m i n a n t s a f f e c t t h e a n a l y t i c a l
column. For best results, replace the guard column at regular
intervals, for example:
After completion of a set of analyses
After running 50 to 100 samples
Weekly
If the quality of your separation deteriorates, or if the initial backpressure increases significantly (more than 20 percent), replace the
guard column. If contaminants appear in the chromatogram, replace
the guard column immediately and follow the column manufacturer’s
recommendations for cleaning the column.
a. Procedure
To replace the guard column:
1. Unscrew the two halves of the holder.
2. Remove the old guard column.
3. Install the new guard column.
Universal Guard Column Holder, Exploded View
Note: Minimize the length of tubing between the guard column and the
analytical column. Ensure that the tubing is seated in the bottom of
the fitting before tightening the compression screw.
4. Reassemble the holder. Hand-tighten only.
5. Equilibrate the analytical column and guard column with five
column volumes of mobile phase. While equilibrating, check the
holder and all connections for leaks.
b. Installing the Integrated Guard Column Holder
Connect the Integrated Guard Column Holder directly to the inlet end
of a cartridge column as shown below.
Integrated Guard Column Holder, Exploded View
Sentry Guard Column 2
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