[ Care and Use ManUal ]
Sentry Guard Columns
Integrated Guard
Column Holder
Universal Guard Column
sentrY GuArd CoLuMn
I. IntroduCtIon
Waters Sentry™ Guard Columns protect your analytical column or
cartridge column from particulate and chemical contamination in
t h e m o b i l e - p h a s e s t r e a m . C o n t a mi n a n t s t h a t m a y d a m ag e y o u r
analytical column are intercepted by the guard column.
Sentry Guard Columns and Guard Holders
Contents
I. IntroduCtIon
a. Packing Materials
II. InstALLInG tHe GuArd CoLuMn
a. Installing the Universal Guard Column Holder
b. Installing the Integrated Guard Column Holder
III. rePLACInG tHe GuArd CoLuMn
a. Procedure
IV. orderInG InForMAtIon
V. WArrAntY/serVICe InForMAtIon
VI. sPeCIFICAtIons
a. Packing Materials
The packing materials used in Waters Sentry guard columns are
identical to the packaging materials used in Waters HPLC columns.
Select guard columns with the same packing materials as your
analytical column.
Sentry Guard Column 1
[ Care and Use ManUal ]
Guard Column Holder
Plastic Bushing
Guard Column
Guard Column Holder
Guard Column Cartridge Column
Spacer
II. InstALLInG tHe GuArd CoLuMn
a. Installing the Universal Guard Column Holder
Install the Universal Guard Column Holder in your HPLC system
between the injector and the column. Use 0.009-inch (0.23 mm) i.d.
tubing for all connections.
III. rePLACInG tHe GuArd CoLuMn
R e p l a c e g u a r d c o l u m n s b e f o r e c o n t a m i n a n t s a f f e c t t h e a n a l y t i c a l
column. For best results, replace the guard column at regular
intervals, for example:
After completion of a set of analyses
After running 50 to 100 samples
Weekly
If the quality of your separation deteriorates, or if the initial backpressure increases significantly (more than 20 percent), replace the
guard column. If contaminants appear in the chromatogram, replace
the guard column immediately and follow the column manufacturer’s
recommendations for cleaning the column.
a. Procedure
To replace the guard column:
1. Unscrew the two halves of the holder.
2. Remove the old guard column.
3. Install the new guard column.
Universal Guard Column Holder, Exploded View
Note: Minimize the length of tubing between the guard column and the
analytical column. Ensure that the tubing is seated in the bottom of
the fitting before tightening the compression screw.
4. Reassemble the holder. Hand-tighten only.
5. Equilibrate the analytical column and guard column with five
column volumes of mobile phase. While equilibrating, check the
holder and all connections for leaks.
b. Installing the Integrated Guard Column Holder
Connect the Integrated Guard Column Holder directly to the inlet end
of a cartridge column as shown below.
Integrated Guard Column Holder, Exploded View
Sentry Guard Column 2
[ Care and Use ManUal ]
IV. orderInG InForMAtIon
The ta ble below lists Sentry guard columns and acc essories.
For complete list of Sentry Guard part numbers, please see Waters
Chromatography Columns and Supplies Catalog (literature code
720002419EN). To order, contact your Waters representative.
Guard Column (2/pkg) Part Number
®
Symmetry
Symmetry C
Nova-Pak
Nova-Pak C
C
18
8
®
C
18
8
Nova-Pak Phenyl WAT046835
Nova-Pak Silica WAT046845
Nova-Pak CN HP WAT046840
™
μBondapak
μBondapak NH
C
18
2
μBondapak Phenyl WAT046850
μBondapak CN WAT046855
™
WAT046860 WAT046860
μPorasil
™
Delta-Pak
Delta-Pak C
Delta-Pak C
Delta-Pak C
Puresil
Resolve
Resolve C
C4, 100 Å WAT046875
, 300 Å WAT046885
4
, 100 Å WAT046880
18
, 300 Å WAT046890
18
™
C
18
™
C
18
8
Resolve CN WAT046925
Accessories and Spare Parts Part Number
Integrated Guard Column Holder WAT046905
Universal Guard Column Holder WAT046910
Compression Screws and Ferrules (5/pkg) WAT025604
Seal, Kalrez
®
WAT054225
WAT054250
WAT044380
WAT046830
WAT044480
WAT046865
WAT046870
WAT046915
WAT046920
WAT023400
V. WArrAntY/serVICe InForMAtIon
Waters Corporation warrants its Guard Columns and Guard Column
Holders in accordance with the following terms and conditions:
Waters will replace without cost any guard column or holder that
fails to perform satisfactorily, if notified within 90 days from your
receipt. Returned columns and holders must have a Return Authorization Number granted by the Waters Customer Service Department.
Approval is subject to the following exclusions:
Physical damage to the guard column or holder because of
misuse or abuse.
Damage to the guard column or holder because of use with
incompatible solvents or use at incorrect temperatures or
pressures.
Failure or d ecrea sed performance as a cons equence of
normal use.
For service and applications questions, contact your Waters
representative.
VI. sPeCIFICAtIons
The table below shows the specifications for Waters Sentry guard
columns and holder assemblies.
Adsorption 4 mg to 40 mg, depending on
nature of contaminants
Pressure Limit 40 MPa (400 Atm, 6000 psi)
Temperature Limit 80 °C
Dead Volume of Guard Column
Holder Assembly
4 μL (plus connecting tubing for
Universal Guard Column)
Sentry Guard Column 3
[ Care and Use ManUal ]
Sales Offices:
Austria and European Export
(Central South Eastern Europe,
CIS and Middle East) 431 877 18 07
Australia 2 9933 1777
Belgium 32 2 726 1000
Brazil 55 11 5094 3788
Canada 800 252 4752
China 8621 6495 6999
CIS/Russia +7 495 3367000
Czech Republic 42 02 617 11384
Denmark 45 46 59 8080
Finland +358 9 5659 6288
France (33) 1 30 48 72 00
Germany 49 6196 400600
Hong Kong 852 29 64 1800
Hungary 36 1 350 5086
India and India Subcontinent
91 80 2 837 1900
Ireland 353 1 448 1500
Italy 02 265 0983
Japan (81) 3 3471 7191
Korea (82) 2 820 2700
Mexico 5255 5200 1860
The Netherlands +31 (0)76-50 87 200
Norway 47 63 84 60 50
Poland (48) 22 833 4400
Puerto Rico 787 747 8445
Singapore 65 6273 1221
Spain 34 93 600 93 00
Sweden 46 8 555 11500
Switzerland 41 56 676 7000
Taiwan 886 2 2543 1898
United Kingdom 44 208 238 6100
© 2008 Waters Corporation. Waters, T he Science of W hat’s
Possible, Sentry, Symmetry, Nova-Pak, μBondapak, μ Porasil,
Delta- Pak, Puresil, and Resolve are trademarks of Waters
Corporation. Kalrez is a registered trademark of E.I. DuPont
deNemours Co.
October 2008 WAT046907 Rev 3 KK-P DF
Sentry Guard Column 4
Waters Corporation
34 Maple Street
Milford, MA 01757 U.S.A.
T: 1 508 478 2000
F: 1 508 872 1990
www.waters.com
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