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[ OPERATOR’S GUIDE ]
Waters Positive Pressure-96 Processor
CONTENTS
I. SYST EM OVE RVIEW
a. Features
II. INSTALLING T HE WATERS POSITIV E
PRESSURE-96 PROCESSOR
a. Installation overview
b. Site requirements
c. Unpacking the processor
d. Installing the processor
III. USING T HE WATERS POSITIV E
PRESSURE-96 PROCESSOR
a. Configuring the trays
b. Plate compression
c. Operating the manifold
d. Controlling positive pressure with flow restrictors
e. Adjusting the extraction flow
IV. MAINTAINING THE WATERS POSITIVE
PRESSURE-96 PROCESSOR
a. Maintenance considerations
b. Replacing the 96-place sealing gasket
c. Cleaning the 96-place sealing gasket
d. Storage requirements for the 96-place sealing gasket
e. Cleaning the device’s exterior
V. A P P EN DIC E S
a. Safety Advisories
i. Warning symbols
ii. Task-specific hazard warnings
iii. Burst Warning
iv. Caution symbol
v. Warnings that apply to all Waters instruments
vi. Handling symbols
b. Spare Parts
c. Safety considerations
i. Safety advisories
d. Operating this device
i. Applicable symbols
ii. Audience and purpose
iii. Intended use of the Waters Positive
Pressure-96 Processor
I. SYST EM OVERVIEW
a. Features
The Waters Positive Pressure-96 Processor offers state-of-the-art
operation for solid-phase extraction (SPE), filtration in plates and
1cc flangless cartridges in 96-well formats. Each of the 96 holes in
the processor is restricted in order to maintain constant pressure,
even if all the plate well positions are not filled. Positive pressure
processing offers many advantages over traditional vacuum
manifold processing.
Positive Pressure-96 Processor.
The advantages of positive pressure processing include:
Reproducible analyte recoveries—the manifold gasket with
96 independent ports provides restricted output of inert gas
at every port. The processor exerts the same motive force to
every port whether it has a full well, dry well or no well access
at all. This provides the most uniform SPE extraction which may
improve the reproducibility of analyte recoveries.
Improved flow for viscous samples—the processor allows for
gas pressures of 60 to 100 psi (optimal gas pressure is 80 psi),
supplying greater motive force for viscous sample flow.
Adjusting the pressure up or down provides smooth, immediate
flow response and high flow control on variable viscosity
plasma samples in a 96-well plate.
Fits all 96-well plate formats—the processor is self-adjusting
to plate height, thus accommodating most 96-well plate formats.
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Easy-to-use design—simply assemble the loaded SPE and
collection plates, and position the assembly on the processor
slide tray platform. Slide the tray to the rear of the processor
until the assembly stops under the manifold. Two toggle
switches on the sides of the unit activate the compression
mechanism. If the user lets go of the buttons too soon, the
manifold mechanism will automatically open. This unique
built-in safety feature is designed to keep the user hands clear
of moving parts during the compression and decompression
cycle, ensuring the safety of the operator.
Superior SPE flow control—the processor exerts fine control
on the SPE flow through the plate. The inert gas flow can be
adjusted by switching the “SPE Flow Rate” selector to the
left (Low Flow setting). The gas delivered to the Positive
Pressure-96 Processor through the adjustable flow regulator
and through the rotometer (on the left). The rotometer is
controlled by the needle valve located at its base. This will
provide a uniform and controlled flow through the entire SPE
96-well plate without the impact on flow from empty wells.
c. Unpacking the processor
The processor is shipped with the following items:
Waters Positive Pressure-96 Processor
Operator’s guide
Installation kit
μElution positive pressure spacer
10 mL x 24 well waste bin tray
1 mL x 96 well collection tray
Quick Start Guide
Handling viscous samples—the gas delivery system can
provide rapid gas flow to the manifold. This flow is controlled
by adjusting the regulator located below the pressure gauge
labeled “Max Flow”. The pressure range is 0 to 100 psi and is
used to maximize flow through the SPE 96-well plate for processing
viscous samples, and significantly reducing the risk of well
plugging by such samples.
II. INSTALLING THE WATERS POSITIVE
PRESSURE-96 PROCESSOR
a. Installation overview
The Waters Positive Pressure-96 Processor is for laboratory use
only. It must be set up inside a hood vent whenever possible and
requires an inert gas supply of 60 to 100 psig.
b. Site requirements
Indoor-use only
Maximum altitude: 2000 meters
Ambient temperature range: 5 to 40 °C
Maximum relative humidity of 80% for temperatures up to 31 °C,
decreasing linearly to 50% relative humidity at 40 °C
Installation Category II
Pollution Degree 2 Environment
Waters Positive Pressure-96 Processor
d. Installing the processor
Compressed nitrogen is the recommended pressure source in order
to ensure inert atmosphere operation.
The Waters Positive Pressure-96 Processor is supplied with an
installation kit which includes:
6 feet of 1/8-inch i.d. (1/4-inch o.d.) polyethylene tubing
Connectors to 1/8-inch NPT or 1/4-inch NPT
Use the tubing and fittings provided to connect the unit to a regulated
oil free gas source.
Regulated source pressure.
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Tip: The optimum source pressure is 80 psig, the minimum pressure
is 60 psig, and the maximum pressure is 100 psig.
Caution: The Waters Positive Pressure-96 Processor does not
include a filter on the source gas input. A clean, oil-free gas source
is essential to prevent sample contamination.
III. USING THE WATERS POSITIV E
PRESSURE-96 PROCESSOR
a. Configuring the plates
The Waters Positive Pressure-96 Processor provides the means to
batch process solid-phase extraction in all Waters 96-well plates,
filtration plates and 1 cc flangeless cartridges in the 96-position
format. The processor accommodates both integrated and modular
plates stacked onto a variety of collection plates. The holder
maintains plate guides for the proper positioning of the collection
plate throughout the sample sequence. Both the holder and
collection plate should be stacked to match precisely so that when
they are correctly aligned, the assembly sits level on the slide
tray platform.
platform. Each column will be pressurized equally, even if wells or
cartridges are omitted. Refer to “Operating the manifold” on page
4 for further explanation. Slide the slide tray to the rear of the
processor until it reaches the stop locating it under the manifold.
The two toggle switches used to activate the compression mechanism
are located on opposite sides of the processor. Both toggle
switches must be pressed at the exact same time to open or
close the compression mechanism. Activate the plate compression
mechanism by depressing the switches until the manifold has
stopped moving (2 to 3 seconds). If you let go too soon, the
manifold mechanism will automatically open. This is a safety
feature designed to keep your hands clear of moving parts during
the compression and decompression cycle.
The Waters 96-well plate formats align to the collection plates
while the Waters μElution plates are placed onto the μElution
positive pressure spacer, and then stacked onto the collection
plate so that sample position for both of these integrated plates
are preserved throughout the sample sequence.
Installing the plate in the Waters Positive Pressure-96 Processor.
b. Plate compression
Assemble the appropriate 96-well plate, μElution plate, or 1 cc
flangeless cartridges and collection plate and position the
assembly on the Waters Positive Pressure-96 Processor slide tray
Sealing the plate.
Decompression of the rack assembly is activated similarly. Both
toggle switches must be depressed simultaneously until the
manifold has stopped moving. Slide the rack assembly forward
until it reaches the stop and proceed with the next step of the method.
The compression speed is pre-set and is not adjustable. The
pressure applied to the compression system is pre-set and is not
adjustable.
c. Operating the manifold
The 96 holes in the processor manifold are restricted in order to
maintain the manifold pressure even if all of the positions are not
filled by columns. With no cartridges in place or if some of the
wells in the plate are empty, there is approximately 0.75 ±.05
scfh of flow through a hole at 25 psig.
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d. Controlling positive pressure with flow restrictors
Inhibiting the flow with the constrictors is helpful in two ways:
The amount of gas used is minimized as compared to a
system with no inhibitors.
The pressure remains high in the wells or cartridges
containing liquid as compared to the empty or dry wells
or cartridges.
e. Adjusting the extraction flow
The Waters Positive Pressure-96 Processor has a two-level gas
delivery system used to pressurize the SPE plate. One part of the
system is controlled by the rotometer located on the left side of
the instrument panel. By switching the “SPE Flow Rate” selector
to the left, “Adjust Flow” setting, the gas is delivered to the
Manifold through the adjustable flow regulator and then through
the rotometer controlled by the needle valve located at its base.
The rotometer provides a very precise and slow flow through
the plate wells or the cartridges by limiting the gas flow to the
manifold from 0 to 2.5 SCFH.
turning the “SPE Flow Rate” selector to off, no gas will flow to the
manifold. It should be in the off position during the compression
and decompression cycles and when not in use.
IV. MAINTAINING THE WATERS POSITIVE
PRESSURE-96 PROCESSOR
a. Maintenance considerations
Safety and handling
Observe these warning and caution advisories when you perform
maintenance operations on your system.
Warning: To prevent injury, always observe Good Laboratory
Practices when you handle solvents, change tubing, or operate the
Waters Positive Pressure-96 Processor. Consult the Material Safety
Data Sheets regarding the solvents you use.
Caution: The Waters Positive Pressure-96 Processor device is constructed
of anodized aluminum, stainless steel, and plastics. To prevent
damage to the device, clean solvent spills and overflow immediately.
A. Manifold Head
B. Toggle Switches
Raise and lower manifold head
(1 on each side)
C. Rotometer
Sets the gas flow for the
low flow setting
D. Low Flow Regulator
E. Max Flow Regulator
F. Selector Valve
Used to select LOW or MAX
gas flow through t he device.
Key Features.
Tip: The rotometer needle valve is for adjustment only and must
never be used as an on off valve. Use the selector valve to turn off
the gas flow.
The second part of the gas delivery system provides for rapid
gas flow to the manifold. This flow is controlled by adjusting the
regulator located below the pressure gauge labeled “Max Flow”.
The pressure range is 0 to 80 psig and is used to maximize
flow through the plate wells or the cartridges for processing
highly viscous samples or to dry the well or cartridge in situ.
By switching the “SPE Flow Rate” selector to “Max Flow”, gas is
introduced to the manifold at the pressure set on the “Max Flow”
regulator as indicated by the gauge located directly above it. By
b. Replacing the 96-place sealing gasket
Required materials
96-place sealing gasket
3/16-inch hex key
To replace the 96-place sealing gasket:
1. Using the enclosed hex key, remove the two 1/4-20 screws
that fasten the manifold to the unit.
2. Lift the manifold off the standoffs and place it upside down on
the table.
Rationale: Removing the manifold helps you accurately align
the replacement 96-place sealing gasket.
3. Remove the old 96-place sealing gasket from the manifold.
4. Attach the enclosed screws to the threaded holes on each
corner on the bottom of the manifold.
5. Remove the backing of the replacement 96-place sealing
gasket. Use the screws for aligning and adhere the replacement
96-place sealing gasket.
6. Once the sealing gasket is attached, remove the four screws.
7. Carefully set the manifold on the standoffs. Fasten the
manifold with the screws.
8. Using the 3/16 hex key, tighten the screws.
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c. Cleaning the 96-place sealing gasket
Use a soft cloth, dampened with methanol, to clean the silicone
rubber sealing gasket.
d. Storage requirements for the 96-place sealing gasket
Store the 96-place sealing gasket at 4 °C. The gasket has a
6 month shelf life from the shipping date.
e. Cleaning the device’s exterior
Use a soft cloth, dampened with water, to clean the outside of the
Waters Positive Pressure-96 Processor.
To clean the surfaces of the device, you should:
Always use eye and hand protection during the
cleaning process.
Apply the water to a clean cloth only, and then wipe the
device down.
Never spray or apply the water directly to any surface
of the device.
V. A P P EN DIC E S
A. Safety Advisories
Waters instruments display hazard symbols designed to alert
you to the hidden dangers of operating and maintaining the
instruments. Their corresponding user guides also include the
hazard symbols, with accompanying text statements describing
the hazards and telling you how to avoid them. This appendix
presents all the safety symbols and statements that apply to the
entire line of Waters products.
Contents:
Warning symbols
Caution symbol
Warnings that apply to all Waters instruments
ii. Task-specific hazard warnings
The following warning symbols alert you to risks that can arise
when you operate or maintain an instrument or instrument
component. Such risks include burn injuries, electric shocks,
ultraviolet radiation exposures, and others.
If the following symbols appear in a manual’s narratives or
procedures, their accompanying text identifies the specific risk
and explains how to avoid it.
Warning: General risk of danger. When this symbol
appears on an instrument, consult the instrument’s user
documentation for important safety-related information
before you use the instrument.
iii. Burst warning
This warning applies to Waters instruments fitted with
nonmetallic tubing.
Warning: Pressurized nonmetallic, or polymer, tubing can
burst. Observe these precautions when working around
suc h tubing:
Wear eye protection.
Extinguish all nearby flames.
Do not use tubing that is, or has been, stressed or kinked.
Do not expose nonmetallic tubing to incompatible compounds
like tetrahydrofuran (THF) and nitric or sulfuric acids.
Be aware that some compounds, like methylene chloride and
dimethyl sulfoxide, can cause nonmetallic tubing to swell,
which significantly reduces the pressure at which the tubing
can rupture.
iv. Caution symbol
The caution symbol signifies that an instrument’s use or misuse can
damage the instrument or compromise a sample’s integrity. The
following symbol and its associated statement are typical of the
kind that alert you to the risk of damaging the instrument or sample.
i. Warning symbols
Warning symbols alert you to the risk of death, injury, or seriously
adverse physiological reactions associated with an instrument’s
use or misuse. Heed all warnings when you install, repair, and
operate Waters instruments. Waters assumes no liability for the
failure of those who install, repair, or operate its instruments to
comply with any safety precaution.
Caution: To avoid damage, do not use abrasives or
solvents to clean the instrument’s case.
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v. Warnings that apply to all Waters instruments
When operating this device, follow standard quality control
procedures and the equipment guidelines in this section.
Attention: Changes or modifications to this unit not
expressly approved by the party responsible for
compliance could void the user’s authority to operate
the equipment.
Important: Toute modification sur cette unité n’ayant pas
été expressément approuvée par l’autorité responsable de
la conformité à la réglementation peut annuler le droit de
l’utilisateur à exploiter l’équipement.
Achtung: Jedwede Änderungen oder Modifikationen an dem Gerät
ohne die ausdrückliche Genehmigung der für die ordnungsgemäße
Funktionstüchtigkeit verantwortlichen Personen kann zum Entzug
der Bedienungsbefugnis des Systems führen.
Avvertenza: qualsiasi modifica o alterazione apportata a questa
unità e non espressamente autorizzata dai responsabili per la
conformità fa decadere il diritto all’utilizzo dell’apparecchiatura
da parte dell’utente.
Atencion: cualquier cambio o modificación efectuado en esta
unidad que no haya sido expresamente aprobado por la parte
responsable del cumplimiento puede anular la autorización del
usuario para utilizar el equipo.
注意：未經有關法規認證部門允許對本設備進行的改變
或修改,可能會使使用者喪失操作該設
備的權利。
注意：未经有关法规认证部门明确允许对本设备进行的
改变或改装，可能会使使用者丧失操
作该设备的合法性。
주의: 규정 준수를 책임지는 당사자의 명백한 승인
없이 이 장치를 개조 또는 변경할 경우,
이 장치를 운용할 수 있는 사용자 권한의 효력을
상실할 수 있습니다.
注意：規制機関から明確な承認を受けずに本装置の変
更や改 造を行うと、本装置のユー
Warning: Use caution when working with any polymer
tubing under pressure:
Always wear eye protection when near pressurized polymer tubing.
Extinguish all nearby flames.
Do not use tubing that has been severely stressed or kinked.
Do not use nonmetallic tubing with tetrahydrofuran (THF) or
concentrated nitric or sulfuric acids.
Be aware that methylene chloride and dimethyl sulfoxide cause
nonmetallic tubing to swell, which greatly reduces the rupture
pressure of the tubing.
Attention: Manipulez les tubes en polymère sous pression avec
precaution:
Portez systématiquement des lunettes de protection
lorsque vous vous trouvez à proximité de tubes en polymère
pressurisés.
Eteignez toute flamme se trouvant à proximité de l’instrument.
Evitez d’utiliser des tubes sévèrement déformés ou endommagés.
Evitez d’utiliser des tubes non métalliques avec du tétrahydrofurane
(THF) ou de l’acide sulfurique ou nitrique concentré.
Sachez que le chlorure de méthylène et le diméthylesulfoxyde
entraînent le gonflement des tuyaux non métalliques, ce qui
réduit considérablement leur pression de rupture.
Vorsicht: Bei der Arbeit mit Polymerschläuchen unter Druck ist
besondere Vorsicht angebracht:
In der Nähe von unter Druck stehenden Polymerschläuchen
stets Schutzbrille tragen.
Alle offenen Flammen in der Nähe löschen.
Keine Schläuche verwenden, die stark geknickt oder
überbeansprucht sind.
Nichtmetallische Schläuche nicht für Tetrahydrofuran (THF)
oder konzentrierte Salpeter- oder Schwefelsäure verwenden.
Durch Methylenchlorid und Dimethylsulfoxid können
nichtmetallische Schläuche quellen; dadurch wird der Berstdruck
des Schlauches erheblich reduziert.
ザーとしての承認が無効になる可能性 があります。
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Attenzione: fare attenzione quando si utilizzano tubi in materiale
polimerico sotto pressione:
Indossare sempre occhiali da lavoro protettivi nei pressi di tubi
di polimero pressurizzati.
Spegnere tutte le fiamme vive nell’ambiente circostante.
Non utilizzare tubi eccessivamente logorati o piegati.
要了解使用二氯甲烷及二甲基亚枫会导致非金属管
线膨胀，大大降低管线的耐压能力。
경고: 가압 폴리머 튜브로 작업할 경우에는
주의하십시오.
가압 폴리머 튜브 근처에서는 항상 보호 안경을
착용하십시오.
Non utilizzare tubi non metallici con tetraidrofurano (THF) o
acido solforico o nitrico concentrati.
Tenere presente che il cloruro di metilene e il dimetilsolfossido
provocano rigonfiamenti nei tubi non metallici, riducendo
notevolmente la pressione di rottura dei tubi stessi.
Advertencia: se recomienda precaución cuando se trabaje con
tubos de polímero sometidos a presión:
El usuario deberá protegerse siempre los ojos cuando trabaje
cerca de tubos de polímero sometidos a presión.
Si hubiera alguna llama las proximidades.
No se debe trabajar con tubos que se hayan doblado o
sometido a altas presiones.
Es necesario utilizar tubos de metal cuando se trabaje
con tetrahidrofurano (THF) o ácidos nítrico o sulfúrico
concentrados.
Hay que tener en cuenta que el cloruro de metileno y el
sulfóxido de dimetilo dilatan los tubos no metálicos, lo que
reduce la presión de ruptura de los tubos.
근처의 화기를 모두 끄십시오.
심하게 변형되거나 꼬인 튜브는 사용하지 마십시오.
비금속(Nonmetallic) 튜브를 테트라히드로푸란
(Tetrahydrofuran: THF) 또는 농축 질산 또는 황산과
함께 사용하지 마십시오.
염화 메틸렌(Methylene chloride) 및 디메틸술폭시드
(Dimethyl sulfoxide)는 비금속 튜브를 부풀려
튜브의 파열 압력을 크게 감소시킬 수 있으므로
유의하십시오.
警告：圧力のかかったポリマー チューブを 扱うときは、
注 意してください。
加圧されたポリマーチューブの付近では、必ず保 護メ
ガネを 着用してください。
近くにある火を消してください。
著しく変 形した、または折れ曲がったチューブは使 用
しないでください。
非金属チューブには、テトラヒドロフラン( T HF )や高
濃度の硝酸または硫酸などを流 さないでください。
警告：當在有壓力的情況下使用聚合物管線時，小心注
意以下幾點。
當接近有壓力的聚合物管線時一定要戴防護眼鏡。
熄滅附近所有的火焰。
不要使用已經被壓癟或嚴重彎曲管線。
不要在非金屬管線中使用四氫呋喃或濃硝酸或濃硫酸。
要了解使用二氯甲烷及二甲基亞楓會導致非金屬管
線膨脹，大大降低管線的耐壓能力。
警告：当有压力的情况下使用管线时，小心注意以下几点：
当接近有压力的聚合物管线时一定要戴防护眼镜。
熄灭附近所有的火焰。
不要使用已经被压瘪或严重弯曲的管线。
不要在非金属管线中使用四氢呋喃或浓硝酸或浓硫酸。
塩化 メチレンやジメチルスルホキシドは、非金 属チュ
ーブの膨張を引き起こす場合が あり、その場合、チュ
ーブは極めて低い圧力で破裂します。
Warning: The user shall be made aware that if the
equipment is used in a manner not specified by the
manufacturer, the protection provided by the equipment
may be impaired.
Attention: L’utilisateur doit être informé que si le matériel est
utilisé d’une façon non spécifiée par le fabricant, la protection
assurée par le matériel risque d’être défectueuses.
Vorsicht: Der Benutzer wird darauf aufmerksam gemacht, dass
bei unsachgemäßer Verwenddung des Gerätes die eingebauten
Sicherheitseinrichtungen unter Umständen nicht ordnungsgemäß
funktionieren.
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Attenzione: si rende noto all’utente che l’eventuale utilizzo
dell’apparecchiatura secondo modalità non previste dal produttore
può compromettere la protezione offerta dall’apparecchiatura.
Advertencia: el usuario deberá saber que si el equipo se utiliza de
forma distinta a la especificada por el fabricante, las medidas de
protección del equipo podrían ser insuficientes.
警告：使用者必須非常清楚如果設備不是按照製造廠
商 指 定 的 方 式使 用，那 麼 該 設 備 所 提 供
的保護將被消弱。
警告：使用者必须非常清楚如果设备不是按照制造厂商
指 定 的方 式使 用，那 么该 设 备所 提 供
的保护将被削弱。
경고: 제조업체가 명시하지 않은 방식으로 장비를
사용할 경우 장비가 제공하는 보호 수단이
제대로 작동하지 않을 수 있다는 점을 사용자에게
반드시 인식시켜야 합니다.
警告： ユーザーは、製造元により指定されていない方
法で機器を使用すると、機器が提供
している保証が無効になる可能性があることに注意し
て下さい。
vi. Handling symbols
These handling symbols and their associated text can appear on
labels affixed to the outer packaging of Waters instrument and
component shipments.
B. Spare Parts
Spare part information is listed in the table below.
Spare parts
Item
Waters Positive
Pressure-96 Processor
96-well plate,
polypropylene
96-well 1 mL
collection plate
Polypropylene cap
mat for 1 mL plate
96-flangeless
cartridges holder
96-place sealing
gasket (special storage
requirement: at 4 °C
and with shelf
life of 6 months)
µElution positive
pressure spacer
Gas supply adapter,
includes 1/8-inch to
1/4-inch NPT fitting,
6 feet of 1/4-inch
tubing
10 mL 24 waste
collection plate
Volume
(mL)
2 mL 50 186002482
1 mL 50 186002481
1 cc 1 186005523
Quantity/
package
- 1 186006961
- 50 186002483
- 1 186005522
- 1 405006528
- 1 186005524
- 1 186005586
Part number
Keep upright!
Keep dry!
Fragile!
Use no hooks!
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C. Safety considerations
Some reagents and samples used with Waters instruments and
devices can pose chemical, biological, and radiological hazards.
You must know the potentially hazardous effects of all
substances you work with. Always follow Good laboratory
Practice and consult your organization’s safety representative
for guidance.
i. Safety advisories
Consult Appendix A for a comprehensive list of warning and
caution advisories.
D. Operating this device
When operating this device, follow standard quality-control (QC)
procedures and the guidelines presented in this section.
i. Applicable symbols
Symbol Definition
Manufacturer
Consult instructions for use
ii. Audience and purpose
This guide is intended for personnel who install, operate, and
maintain the Waters Positive Pressure-96 Processor.
iii. Intended use of the Waters Positive Pressure-96 Processor
The Waters Positive Pressure-96 Processor is for research use only
and is not intended for use in diagnostic applications.
Waters, and The Science of W hat’s Possible are registered
trademarks of Waters Corporation. All other trademarks are
the property of their respective owners.
©2014 Waters Corporation. Produced in the U.S.A.
February 2014 715002662EN K P-PDF
Waters Corporation
34 Maple Street
Milford, MA 01757 U.S.A.
T: 1 508 478 2000
F: 1 508 872 1990
www.waters.com
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