Waters NanoEase Trapping Columns User Manual

[ Care and Use ManUal ]

nanoEasE trappinG columns

i. introduction:

Waters NanoEase Trapping Columns are robust devices that can effectively
separate complex samples and remove buffers and high concentrations of
salts. For the latest on Life Science Chemistries, go to:

www.waters.com/lifesciences.

ii. nanoEasE trap column prEparation

1. It is recommended that the trap column be washed and conditioned

with a suitable solvent before use. This can be accomplished using

a suitable syringe to flush solvent through the column. If a syringe

is used for this purpose, it is recommended that a syringe with an

inert teflon plunger and glass body be used to avoid contami-

nation. Syringes with rubber plungers or plastic bodies should

not be used. Alternatively, the trap column can be placed on the

HPLC system and washed with suitable solvent at a flow rate of

10-40 µL/min for several minutes prior to use.

®

2. If using a 10-port valve, use UpChurch

part # F-285X) in eac h adjacent port of the 10-port valve, due to

the clearance width of the trap column.

long nuts (UpChurch

contEnts

i. introduction

ii. nanoEasE trap column prEparation

iii. GEnEral GuidElinEs for column usE

iv. ordErinG information

3. The trap column works with the black 10-32 outer fitting with

white label (Figures 1a and 1b), which secures the stainless steel

®

tu be and the tan P EEK

switching valve.

NanoEase Trapping Columns 1

(column insert part) pie ce ( Figure 2) to the

[ Care and Use ManUal ]

4. To secure the column into a multi-port valve, care must be taken

to get the face of the stainless steel barrel seated properly into

the port without introducing dead volume between the frit at the

end of the stainless steel barrel and the valve port. The stainless

steel barrel must not protrude too far past the end of the black

fitting, otherwise there will not be enough threads of the fitting

protruding into the valve port to make a proper seal. Consult Fig-

ures to see how to properly seal the trap into the valve port and

connect the desired tubing. The following steps are suggested:

a) First make sure that the trap column insert (Figure 2) is

inside the black fitting (Figure 1b).

b) Screw in a column-blanking plug into the top of the black

fitting (Figure 3) until the stainless steel barrel protrudes

from the end of the fitting approximately between 2 and 3

mm (Figure C). It may be necessary to use your finger (use

a glove to avoid contamination of the trap) to push against

the end of the stainless steel barrel so that the other end of

the insert is set against the column-blanking plug and the

stainless steel barrel protrudes from the end of the black

fitting at the proper depth. Adjust the position of the col-

umn-blanking plug if necessary.

a. Illustration of adjusting the length of the stainless
steel barrel

Figure A. Incorrect. Results in dead volume between valve port and trap column

Figure 1a

Figure 3

Figure 2

Figure 1b

Figure B. Incorrect. Note these are insufficient number of threads to seal trap

column to valve

2-3 mm

Figure C. Correct.

NanoEase Trapping Columns 2