Waters nanoACQUITY UPLC 2G Trap Columns User Manual
[ CARE AND USE MANUAL ]
nanoACQUITY UPLC 2G Trap Column
TABLE OF CONTENTS
I. INTRODUCTION
II. FITTINGS
III. CONNECTING THE COLUMN TO THE
UPLC SYSTEM
IV. COLUMN USAGE
a. Mobile Phases
b. Sample Considerations
c. Sample Load And Flow Rates
d. Removal
e. Recommended Storage
V. ORDERING INFORMATION
I. INTRODUCTION
Thank you for choosing a nanoACQUITY UPLC® 2G Trap Column.
nanoACQUITY UPLC Trapping Columns are manufactured in a cGMP,
ISO 9001:2000 certified plant with each step being conducted
within narrow tolerances. Every column is individually tested and
Certificates of Batch Analysis and a Performance Chromatogram
are provided with each trapping column.
The nanoACQUITY UPLC 2G Trap Column (10,000 psi) utilize a
proprietary device designed to minimize peak broadening while
maximizing chromatographic efficiency. This can be used in a
variety of 1D nano flow applications.
[ CARE AND USE MANUAL ]
nanoACQUITY 10-32/V-Detail
(10,000 psi rated)
nanoACQUITY 6-40/M-Detail
(10,000 psi rated)
3/16” Gold-plated
compression screw
Steel sleeve
One-piece ferrule
nanoACQUITY 6-40/M-Detail
Analytical columnnano TeeTrap column
Needle
Waste
Figure 1. Trap column on a nanoACQUITY UPLC single pump configuration.
In order to achieve the highest performance from your
nanoACQUITY UPLC 2G Trap Column investment, we strongly
suggest you observe the care and use guidelines detailed below.
II. FITTINGS
Depending on your Waters nanoACQUITY UPLC System and
application needs, the nanoACQUITY UPLC 2G Trap Column will
utilize two of the connector fittings shown in Figure 2.
III. CONNECTING THE COLUMN TO THE UPLC SYSTEM
These procedures outline the steps necessary to install your
nanoACQUITY UPLC 2G Trap Column (10,000 psi) onto a
nanoACQUITY UPLC System. Connecting and installing your
purchased trap properly is essential to minimizing dead volumes
and maximizing chromatographic performance. Make sure that all
connected trap fittings are bottomed into all ports and tees.
1. Start the fitting into the port.
2. Grasp the end fitting and gently push it into the appropriate
nanoACQUITY UPLC tee, port, or valve. Note that bottoming of
the Trap tubing into the port minimizes system dead volume.
3. Tighten the fitting according to the recommendations in the
following table.
If the V-Detail fitting is new and not yet installed, tighten
the fitting snug (finger-tight) plus one full turn (360 degrees)
using a 1/4-inch wrench.
If the M-Detail fitting is new and not yet installed, tighten
the fitting snug (finger-tight) plus 1/2 full turn (180 degrees)
using a 3/16-inch wrench.
1/4” Gold-plated
compression screw
Double-crimped
stainless steel sleeve
(10,000 psi rated)
3/16” Gold-plated
compression screw
Steel sleeve
Figure 2: nanoACQUITY UPLC 2G Trap Column (10,000 psi) M-detail and
V-detail connection fittings.
Two-piece ferrule
One-piece ferrule
If either V-Detail or M-Detail fitting has been installed
and removed once or more, tighten the fitting snug (finger-
tight) plus 1/6 turn (60 degrees) using the correct wrench as
detailed above. Proper 60 degree final tightening is used for
both the V-Detail and M-Detail fittings when they have been
previously used.
IV. COLUMN USAGE
a. Mobile Phases
Use only filtered and degassed mobile phases that are compatible
with the column packing material. Switch only between mutually
miscible mobile phases to prevent possible precipitation in the
nanoACQUIT Y UPLC System lines and/or within trap columns.
Note: the Symmetry® C18, 5 µm, 2G Trap Column is shipped in 100%
Optima™ Acetonitrile (Fisher Scientific).
b. Sample Considerations
The presence of particulates in the injected sample will greatly
affect trap column life. Whenever possible, inject only filtered or
microcentifuged (e.g., 10,000 psi for 5 minutes) samples contained
in eluent that are miscible with the separation mobile phases.
nanoACQUITY UPLC 2G Trap Columns
2
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