Waters nanoACQUITY Columns 5000 psi User Manual
Reordering information.
The table below provides part numbers for currently available
NanoACQUITY
™
Columns. Additional separations chemistries,
column configurations, and connecting hardware (e.g., PEEK
fittings) are available as custom requests.
Waters Part Column style Material Particle Size I.D. (micron) Length (cm)
Number (micron)
186002821 NanoACQUITY™Symmetry®C
186002822 NanoACQUITY™Symmetry®C
186002823 NanoACQUITY™Symmetry®C
186002824 NanoACQUITY™Symmetry®C
186002825 NanoACQUITY™Symmetry®C
186002826 NanoACQUITY™Symmetry®C
186002827 NanoACQUITY™Symmetry®C
186002828 NanoACQUITY™Symmetry®C
186002829 NanoACQUITY™Atlantis®dC
186002830 NanoACQUITY™Atlantis®dC
186002831 NanoACQUITY™Atlantis®dC
186002832 NanoACQUITY™Atlantis®dC
186002833 NanoACQUITY™Atlantis®dC
186002834 NanoACQUITY™Atlantis®dC
186002835 NanoACQUITY™Atlantis®dC
186002836 NanoACQUITY™Atlantis®dC
186002837 NanoACQUITY™BEH C
186002838 NanoACQUITY™BEH C
186002839 NanoACQUITY™BEH C
186002840 NanoACQUITY™CUSTOM
™
186002841
NanoACQUITY
Trap column
Symmetr
3.5 75 10
8
1
3.5 75 15
8
1
3.5 100 10
8
1
3.5 100 15
8
1
3.5 150 10
8
1
3.5 150 15
8
1
3.5 300 10
8
1
3.5 300 15
8
1
3 75 10
18
3 75 15
18
3 100 10
18
3 100 15
18
3 150 10
18
3 150 15
18
3 300 10
18
3 300 15
18
1.7 75 10
18
1.7 100 10
18
1.7 150 10
18
y C
5 180 2
18
S
ales Offices:
A
ustria and European Export (Central South
E
astern Europe, CIS and Middle East)
(43) 1 8771807
Australia (61) 2 9933 1777
Belgium (32) 2 7261000
Brazil (55) 11 5543 7788
Canada 800 252 4752
China (8610) 84518918
CIS/Russia (7) 095 336 7000
C
zech Republic (42) 02 6171 1384
Denmark (45) 46 598080
F
inland (358) 9 506 4140
France (33) 1 30 48 72 00
Germany (
49) 6196 40 06 00
Hong Kong (852) 2964 1800
Hungary (36) 1 350 5086
India and India Subcontinent
(91) 80 28371900
Ireland (353) 1 448 1500
Italy (39) 02 27421.1
Japan (81) 3 3471 7191
Korea (82) 2 820 2700
Mexico (5255) 5524 76 36
The Netherlands 31 (0)76-50 87 200
way
Nor
(47) 63 84 60 50
Poland (48) 22 833 4400
to Rico
Puer
(787) 747 8445
Singapore (65) 6278 7997
Spain (34) 93 600 93 00
Sweden (46) 8 555 11500
Switzerland (41) 62 889 2030
Taiwan (886) 2 2543 1898
United Kingdom (44) 208 238 6100
All other countries:
Waters Corporation U.S.A.
508 478 2000/800 252 4752
NanoACQUITY™Columns
Care and Use Instructions
™
NanoACQUITY
NanoACQUITY™Capillary Columns
Nano Columns
Waters Corporation 34 Maple Street, Milford, MA 01757 USA
www.waters.com
December, 2004
©2004 Waters Corporation
Waters, Atlantis,Symmetry, XTerra and NanoACQUITY
are trademarks of Waters Corporation.
N
anoACQUITY
™
C
OLUMNS CARE AND USE:
NanoACQUITY™columns are manufactured to exacting specifications,
which provide unmatched peak symmetry for maximum sensitivity
and accurate quantitation. As the columns are fritted at both ends,
they are able to withstand pressure changes during injection and
thus provide superior longevity. In order to achieve maximum
performance, it is important to ensure that proper connections are
made to minimize peak tailing and poor efficiency.
CONTENTS:
• NanoACQUITY™Column
• Care and Use Instruction Sheet – This instruction sheet contains
important information about using and maximizing performance
from your NanoACQUITY
™
Column.
• Certificate of Batch Analysis – Demonstrates the results of the
physical and chemical characterization tests performed on the
batch of chromatographic packing in your NanoACQUITY
™
column.
• Certificate of Column Performance – Demonstrates a
chromatographic separation of a peptide mixture fr
om a
representative column.
COLUMN DESCRIPTIONS:
The NanoACQUITY™column you have purchased contains several
innovative features designed to provide maximum performance,
ease-of use and longevity:
75 µm, 100 µm, and 150 µm Column
1) Optimized column packing protocol to provide superior
column efficiency.
2) Diamond-polished column ends and connecting tubing to
provide minimum extra-column peak broadening.
3) Proprietary fritting technology to provide stable, long-lasting
column (patent pending).
4) Special fitting to connect to a NanoACQUITY
™
system.
5) Protective PTFE oversleeve for easier handling and more
rugged column design (patent pending).
6) 500 cm of 20 µm ID connection tubing coupled to column
outlet with zero-dead-volume PTFE coupler to maintain
chromatographic performance while simplifying column set-up.
o-dead volume PTFE pr
7) Zer
otector/coupler for joining column
to electrospray emitter top or other detector.
300 µm Column
1) Optimized column packing protocol to provides superior
c
olumn efficiency
2) Low volume Valco style end fitting for minimum band-broadening
and ease of use
GENERAL COLUMN INSTALLATION:
NOTE: Take care in handling NanoACQUITY™columns to prevent
inadvertent damage. With the 75 µm, 100 µm, and 150 µm
columns, do not bend the PTFE oversleeve, and do not pull the
capillary connection tubing away from the column coupling.
Flow mobile phase only in the direction indicated by the arrow
on the column label. Connect the column with the direction of
the flow arrow on the label pointing to the detector or Mass
Spectrometer.
75 µm, 100 µm, and 150 µm Installation Column:
1) Remove the protective tube from the inlet end of the column. Connect
™
the column inlet to the NanoACQUITY
2) For connecting the NanoACQUITY
system. Do not overtighten.
™
column to your electrospray
emitter or other detector connection tubing, Waters recommends
that you use the supplied PTFE zero-dead volume coupler to connect
your column to the detector. Firmly grasp the coupler that is attached
to the connecting tubing, and slide it down the tubing until the tube
end is positioned approximately halfway inside the coupler. If you
have access to a low power microscope, use it to examine the end
of the emitter to be inserted in the coupler to ensure that there is
no particulate contamination. Remove contaminants by wiping
carefully with a Kim-wipe moistened with methanol. While grasping
the coupler, carefully insert and slide your electrospray emitter in
the coupler until the emitter and connecting tubing are butted
against each other. Inspect the union to ensure that both ends are
butted together properly. If you observe shavings of PTFE between
the connections, remove the emitter and slide the connecting tubing
through the coupler to remove the pieces of PTFE. Repeat the procedure
described above to re-connect the connector to the emitter. This
approach will provide the lowest dead volume connection and
will result in the best chromatographic peak shape.
Alter
natively
, you can r
emove the coupler and connect your column to
the detector using commercially available microtight connectors. However,
this approach can result in poor chromatographic performance because
there can be significant unswept dead volume.
300 µm Installation Column:
1) Connect the column inlet to the injector utilizing a Valco style
c
ompression screw.
2) Connect the column outlet to the detector or Mass Spectrometer
using a Valco style compression screw.
COLUMN MAINTENANCE:
Caring for the column
To ensure continued high performance of your NanoACQUITY
™
column, follow these guidelines:
1) Always use filtered mobile phases.
2) Filter or centrifuge particulate-laden samples.
3) Do not subject the column to sudden changes of pressure or flow.
Cleaning and regenerating the column
A shift in retention or resolution may indicate contamination of the
column. Flushing with a neat organic solvent is usually sufficient to
remove the contaminant. If system backpressure increases with usage,
the column inlet is most likely becoming plugged with particulates
from the mobile phase or sample. Use an appropriate solvent to
dissolve the precipitate. For example, proteinaceous material can
emoved with repeated injections of neat DMSO.
be r
Column storage
For overnight storage, continuously flush the column with the mobile
phase at 10 - 20% of the maximum recommended flow rate. Store
the column in the HPLC grade water when it will be used within
24 hrs or in 100% acetonitrile for long term storage. (Caution:
Rinse column in HPLC grade water before switching to 100%
organic solvent to avoid potential salt precipitation in column.)
SAFETY:
Use appropriate safety glasses or goggles meeting ANSI Z87.11989 requirements when handling fused silica tubing.
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