Waters MassPREP E. coli Digestion Standard User Manual

[ Care and Use ManUal ]

MASSPREP E. COLI DIGESTION STANDARD

I. INTRODUCTION

The MassPREP™ E.coli digestion standard (MPDS E.coli) is intended

to provide a complex biological matrix for producing samples used

to train new users of the Waters Protein Expression System. MPDS

E. coli was prepared by digesting a purified E. coli cytosolic protein

fraction with sequencing grade trypsin. T he MPDS mixture is purified,

and does not contain undigested protein, trypsin, or very hydrophilic

components such as salts.

II. VIAL CONTENTS

Each vial contains 100 µg of lyophilized E. coli cytosolic protein

digest.

III. STORAGE AND STABILITY

The lyophilized powder is stable at room temperature until the

expiration date printed on the label, or one year from purchase. Once

reconstituted in 0.1% formic acid, the solution is stable for one week

when stored at 4 °C. Stability can be extended by freezing samples at

-20 °C.

CONTENTS

I. INTRODUCTION

II. VIAL CONTENTS

III. STORAGE AND STABILITY

IV. RECONSTITUTION OF MPDS E. COLI FOR ANALYSIS WITH THE

WATERS PROTEIN EXPRESSION SYSTEM USING NANOSCALE
(75 m scale) HPLC

V. RECONSTITUTION OF MPDS E. COLI FOR ANALYSIS WITH THE

WATERS PROTEIN EXPRESSION SYSTEM USING MICROSCALE
(300 m scale) HPLC

VI. SAFETY AND MSDS INFORMATION

VII. MASSPREP DIGESTION STANDARD ORDERING INFORMATION

Note: The contamination of sample with endo- or exopeptidases may

cause rapid sample degradation.

IV. RECONSTITUTION OF MPDS E. COLI FOR ANALYSIS WITH THE
WATERS PROTEIN EXPRESSION SYSTEM USING NANOSCALE (75
m scale) HPLC

One vial of MPDS E. coli is required to execute the Waters Protein

Expression System training module. MPDS E. coli is prepared by resus-

pension of the lyophilized powder in 250 µL of 0.1% formic acid. Mix

capped vial by vortexing to insure all material is resuspended. Refer to

the Waters Protein Expression System Operator’s Guide for details on

the use of the MPDS E. coli mixture for system training purposes.

E. Coli Digestion Standard 1

[ Care and Use ManUal ]

V. RECONSTITUTION OF MPDS E. COLI FOR ANALYSIS WITH THE
WATERS PROTEIN EXPRESSION SYSTEM USING MICROSCALE
(300 m scale) HPLC

Two vials of MPDS E. coli are required to execute the Waters Protein

Expression System training module. The lyophilized powder in the first

vial should be resuspended in 50 µl of 0.1% formic acid. The resulting

solution in the first vial should be transferred, via pipette, to the sec-

ond vial, and used to resuspend the remaining lyophilized powder. Mix

capped vial by vortexing to insure all material is resuspended. Refer to

the Waters Protein Expression System Operator’s Guide for details on

use the of the MPDS E. coli mixture for system training purposes.

VI. SAFETY AND MSDS INFORMATION

MPDS mixtures contain various non-hazardous peptides in powder

form. They are for laboratory use only; and solely intended for

purposes of scientific experimentation, analysis, research, or develop-

ment. The mixtures should be used only as directed and in accordance

with good laboratory practice. All Waters MassPREP Digestion Stan-

dards are non hazardous as defined by OSHA’s Hazard Communication

Standard, 29 CFR 1910.1200(c) and Appendix A and B, and are thus

exempt from the requirements of the Hazard Communication Standard,

including the requirement to supply MSDSs.

VII. MASSPREP DIGESTION STANDARD ORDERING INFORMATION

Item Part Number Quantity

MassPREP Digestion Standard, Enolase 186002325 1

MassPREP Digestion Standard, Phosphorylase b 186002326 1

MassPREP Digestion Standard,
Bovine Hemoglobin

MassPREP Digestion Standard, Alcohol
Dehydrogenase

MassPREP Digestion Standard,
Bovine Serum Albumin

MassPREP Digestion Standard Kit
(one of each std.)

MassPREP Digestion Standard, Mix 1 186002865 1

MassPREP Digestion Standard, Mix 2

186002327 1

186002328 1

186002329 1

186002330 5

186002866

1

E. Coli Digestion Standard 2