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MASSPREP E. COLI DIGESTION STANDARD
I. INTRODUCTION
The MassPREP™ E.coli digestion standard (MPDS E.coli) is intended
to provide a complex biological matrix for producing samples used
to train new users of the Waters Protein Expression System. MPDS
E. coli was prepared by digesting a purified E. coli cytosolic protein
fraction with sequencing grade trypsin. T he MPDS mixture is purified,
and does not contain undigested protein, trypsin, or very hydrophilic
components such as salts.
II. VIAL CONTENTS
Each vial contains 100 µg of lyophilized E. coli cytosolic protein
digest.
III. STORAGE AND STABILITY
The lyophilized powder is stable at room temperature until the
expiration date printed on the label, or one year from purchase. Once
reconstituted in 0.1% formic acid, the solution is stable for one week
when stored at 4 °C. Stability can be extended by freezing samples at
-20 °C.
CONTENTS
I. INTRODUCTION
II. VIAL CONTENTS
III. STORAGE AND STABILITY
IV. RECONSTITUTION OF MPDS E. COLI FOR ANALYSIS WITH THE
WATERS PROTEIN EXPRESSION SYSTEM USING NANOSCALE
(75 m scale) HPLC
V. RECONSTITUTION OF MPDS E. COLI FOR ANALYSIS WITH THE
WATERS PROTEIN EXPRESSION SYSTEM USING MICROSCALE
(300 m scale) HPLC
VI. SAFETY AND MSDS INFORMATION
VII. MASSPREP DIGESTION STANDARD ORDERING INFORMATION
Note: The contamination of sample with endo- or exopeptidases may
cause rapid sample degradation.
IV. RECONSTITUTION OF MPDS E. COLI FOR ANALYSIS WITH THE
WATERS PROTEIN EXPRESSION SYSTEM USING NANOSCALE (75
m scale) HPLC
One vial of MPDS E. coli is required to execute the Waters Protein
Expression System training module. MPDS E. coli is prepared by resus-
pension of the lyophilized powder in 250 µL of 0.1% formic acid. Mix
capped vial by vortexing to insure all material is resuspended. Refer to
the Waters Protein Expression System Operator’s Guide for details on
the use of the MPDS E. coli mixture for system training purposes.
E. Coli Digestion Standard 1
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V. RECONSTITUTION OF MPDS E. COLI FOR ANALYSIS WITH THE
WATERS PROTEIN EXPRESSION SYSTEM USING MICROSCALE
(300 m scale) HPLC
Two vials of MPDS E. coli are required to execute the Waters Protein
Expression System training module. The lyophilized powder in the first
vial should be resuspended in 50 µl of 0.1% formic acid. The resulting
solution in the first vial should be transferred, via pipette, to the sec-
ond vial, and used to resuspend the remaining lyophilized powder. Mix
capped vial by vortexing to insure all material is resuspended. Refer to
the Waters Protein Expression System Operator’s Guide for details on
use the of the MPDS E. coli mixture for system training purposes.
VI. SAFETY AND MSDS INFORMATION
MPDS mixtures contain various non-hazardous peptides in powder
form. They are for laboratory use only; and solely intended for
purposes of scientific experimentation, analysis, research, or develop-
ment. The mixtures should be used only as directed and in accordance
with good laboratory practice. All Waters MassPREP Digestion Stan-
dards are non hazardous as defined by OSHA’s Hazard Communication
Standard, 29 CFR 1910.1200(c) and Appendix A and B, and are thus
exempt from the requirements of the Hazard Communication Standard,
including the requirement to supply MSDSs.
VII. MASSPREP DIGESTION STANDARD ORDERING INFORMATION
Item Part Number Quantity
MassPREP Digestion Standard, Enolase 186002325 1
MassPREP Digestion Standard, Phosphorylase b 186002326 1
MassPREP Digestion Standard,
Bovine Hemoglobin
MassPREP Digestion Standard, Alcohol
Dehydrogenase
MassPREP Digestion Standard,
Bovine Serum Albumin
MassPREP Digestion Standard Kit
(one of each std.)
MassPREP Digestion Standard, Mix 1 186002865 1
MassPREP Digestion Standard, Mix 2
186002327 1
186002328 1
186002329 1
186002330 5
186002866
1
E. Coli Digestion Standard 2
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© 2008 Waters Corporation. Waters, T he Science of
What’s Possible.
January 2009 715001702 Rev B KK/PDF
Waters Corporation
34 Maple Street
Milford, MA 01757 U.S.A.
T: 1 508 478 2000
F: 1 508 872 1990
www.waters.com
E. Coli Digestion Standard 3
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