Waters HSPgel Columns User Manual

[ Care and Use ManUal ]

HsPgel Columns For HIgH-sPeed gPC analysIs

Contents

I. IntroduCtIon

II. HsPgel Column FamIlIes

III. ConneCtIng tHe Column to tHe gPC system

a. Column Connectors

b. Connecting an HSPgel Column

c. Solvents

IV. Column temPerature ranges

I. IntroduCtIon

Thank you for choosing a Waters HSPgel™ column. Every column

is individually tested and Certificates of Batch Analysis and a

Performance Chromatogram are provided with each column. It is not

recommended to use a guard column with the HSPgel columns, as it

will add significant volume and possible additional bandspreading

to the system. It is recommended, however, that a ~0.5 µm in-line

filter (pre-column) be installed to trap any particulates that may

potentially plug the column frit. The GPC analysis solvent must

be filtered prior to use through an appropriate ~0.5 µm filter. The

sample solutions should also be filtered prior to injection, unless the

sample molecular weight is very high and there may be a chance of

shear degradation.

HSPgel Columns for High Speed GPC Analysis 1

[ Care and Use ManUal ]

II. HsPgel Column FamIlIes

HSPgel columns are available in four offerings, three for organic GPC and

one for aqueous GPC. All columns have dimensions of 6.0 x 150 mm.

The descriptions of the columns are as follows:

• HSPgel HR series for room temperature, high resolution GPC

analysis of organic polymers. Shipped in THF and one solvent

changeover only.

• HSPgel RT series for routine room temperature GPC analysis

of organic polymers. Shipped in THF and multiple solvent

changeovers allowed.

• HSPgel HT series for GPC analysis of organic polymers from

room temperature to 180 °C. Shipped in either THF or ODCB and

may be transferred multiple times to other solvents (i.e., TCB at

0.15 m L /mi n) .

• HSPgel AQ series for room temperature analysis of aqueous

GPC analysis of water soluble polymers.

III. ConneCtIng tHe Column to tHe gPC system

Handle the column with care. Do not drop or hit the column on a hard surface

as it may disturb the bed and affect its performance.

1. Correct connection of 1/16-inch outer diameter stainless steel

tubing leading to and from the column is essential for high-quality

chromatographic results.

2. When using standard stainless-steel compression screw fittings, it is

important to ensure proper fit of the 1/16-inch outer diameter stain-

less steel tubing. When tightening or loosening the compression screw,

place a 5/16-inch wrench on the compression screw and a 3/8-inch

wrench on the hex head of the column endfitting.

Note: If one of the wrenches is placed on the column flat during this

process, the endfitting will be loosened and leak.

3. If a leak occurs between the stainless steel compression screw fitting

and the column endfitting, a new compression screw fitting, tubing and

ferrule must be assembled.

4. An arrow on the column identification label indicates correct direction

of solvent flow.

Table 1. Specifications for HSPgel Columns

Application Columns

Ultra-Hgh

Resolution

GPC

Room

Temperature

GPC

High

Temperature

GPC

Aqueous AQ 2.5 16,000 500- 2,000 4

* MW ranges base d on polystyrene c hain lengths for HR, RT and HT series, based on polyeth-

ylene oxide chain.

** Exclusion limits for AQ series extrapolated from highest MW polyethlene oxide standard

(~900,000 MW).

HR 1.0 18,000 100- 1,000 3

HR 2.0 18,000 500- 10,000 3

HR 2.5 18,000 1,000- 20,000 3

HR 3.0 18,000 2,000- 60,000 3

HR 4.0 18,000 10,000- 400,000 3

HR MB-L 18,000 500- 700,000 3

HR MB-M 16,000 1,000- 4,000,000 Mix of 3.5

HR MB-H 10,000 5,000- 10,000,000 10

RT 1.0 16,000 100- 1,000 3

RT 2.0 16,000 500- 10,000 3

RT 2.5 16,000 1,000- 20,000 3

RT 3.0 16,000 2,000- 60,000 3

RT 4.0 16,000 10,000- 400,000 3

RT 5.0 16,000 25,000- 4,000,000 3

RT 6.0 10,000 50,000- 10,000,000 3

RT 7.0 10,000 100,000- 15,000,000 3

RT MB-L 16,000 100- 1,000 3

RTMB-L/M 16,000 500- 400,000 3

RTMB-M 16,000 1,000- 4,000,000 3

RTMB-H 10,000 5,000- 10,000,000 Mix of 3.5

HT 1.0 12,000 100-1.000 5

HT 2.0 12,000 500- 10,000 5

HT 2.5 12,000 1,000- 20,000 5

HT 3.0 12,000 2,000- 60,000 5

HT 4.0 12,000 10,000- 400,000 5w

HT 5.0 12,000 25,000- 4,000,000 5

HT 6.0 8,000 50,000- 10,000,000 5

HT 7.0 8,000 100,000- 15,000,000 5

HT MB-L 12,000 100- 1,000 5

HTMB-L/M 12,000 500- 400,000 5

HTMB-M 12,000 1,000- 4,000,000 5

HTMB-H 8,000 5,000- 10,000,000 5

AQ 3.0 16,000 1,000- 60,000 4

AQ 4.0 10,000 10,000- 400,000 6

AQ 5.0 10,000 50,000- 4,000,000** 7

AQ 6.0 7,000 100,000-

AQ MB-H 7,000 500- 10,000,000** 9

Theoretical

Plates

MW Range*

10,000,000**

Particle

Size (µm)

9

HSPgel Columns for High Speed GPC Analysis 2