[ Care and Use ManUal ]
gUaRD-pak hOlDeR aND INseRTs
I. INTRODUCTION
The Waters Guard-Pak™ protects your HPLC column from particulate and
chemical contamination in the mobile phase stream. The Waters Guard-Pak
assembly consists of a holder and a disposable insert.
CONTeNTs
I. INT RODUCTION
a. Sorbents
b. Precautions
II. INsTallINg The gUaRD-pak
a. Conditioning the Insert
b. Assembling the Guard-Pak
c. Installing the Assembled Guard-Pak
III. ReplaCINg The INseRT
Iv. speCIfICaTIONs
v. ORDeRINg INfORmaTION
vI. WaRRaNTy/seRvICe INfORmaTION
Guard-Pak Holder and Inserts
Guard-Pak Holder and Inserts 1
[ Care and Use ManUal ]
a. Sorbents
The sorbents used in Guard-Pak inserts are identical to the sorbents used in
Waters HPLC columns and cartridges. Select an insert with the same sorbent
as your column or cartridges. Use the Nova-Pak® Silica insert when using a
μPorasil™ column or cartridge.
a. Precautions
When using a Guard-Pak insert with high performance/low volume columns,
some loss of efficiency may result. When using the Guard-Pak holder and
insert, observe the same precautions that you follow with an analytical column. To prevent precipitation inside the insert, use only miscible eluents in
series.
II. INsTallINg The gUaRD-pak
To install the Guard-Pak on your HPLC system:
• If necessary, condition the insert
• Assemble the Guard-Pak
• Install the assembled Guard-Pak holder
a. Conditioning the Insert
1. Use 0.009-inch (0.23 mm) i.d. tubing to connect the Guard-Pak
assembly to your system. Use the fittings included in the kit to
make the extra connections.
2. Connect the Guard-Pak inlet to the injector and the outlet unit to
the column or cartridge.
Figure 1: System with Guard-Pak Holder and Insert (Exploded View)
Keep the tubing between the holder and the column as short as possible.
Ensure that the tubing is seated in the bottom of the fitting before tightening
the compression screw.
III. ReplaCINg The INseRT
Replace Guard-Pak inserts before contaminants appear in the chromatogram.
For best results, replace the insert:
Before use, condition dry-packed inserts (Nova-Pak®, Resolve™, μBondapak®,
and Delta-Pak™). Polymer-based Guard-Pak inserts are shipped wet and does
not require conditioning.
Remove and discard the polyethylene foam shipping plug.
To condition the insert, either:
• Soak the insert for 2 minutes in 5 mL of the organic component of
the mobile phase.
• Assemble the Guard-Pak. Run 5 mL of the organic component of the
mobile phase through the assembled Guard-Pak and out to waste.
b. Assembling the Guard-Pak
Place the Guard-Pak insert on the outlet unit and place the Guard-Pak holder
inlet and collar on top. Hand-tighten only.
c. Installing the Assembled Guard-Pak
This procedure requires a 5/16-inch wrench. To install:
Ensure that the spiral-cut distributor disk in the outlet unit was not dislodged
during shipping.
• After completion of a set of analyses
• After running 50 to 100 samples
• When the initial backpressure increases significantly
Procedure:
To replace the Guard-Pak insert:
1. Unscrew the inlet unit from the outlet unit. Hold the outlet unit to
prevent the injector tubing from twisting.
2. Use the insert removal tool to remove the old insert.
Figure 2: Using the Insert Removal Tool
Guard-Pak Holder and Inserts 2
[ Care and Use ManUal ]
Be careful not to damage the O-ring or metal seat. Insert the tool into
the cavity of the insert only; do not pry from between the insert and
metal flange of the outlet unit.
3. Condition the new insert, if necessary.
4. Install the new Guard-Pak insert on the insert mount. Inspect the
O-ring when you replace the insert.
5. Reassemble the holder. Hand-tighten only. Check the assembled
holder for leaks while it is under pressure.
Iv. speCIfICaTIONs
Table 1 shows the specifications for a Waters Guard-Pak assembly.
Table 1: Waters Guard-Pak Specifications
Adsorption 1 mg to 10 mg, depending on contaminants
Pressure Limit 40 Mpa (400 Atm, 6000 psi)
Amount of Packing 50-100 mg, depending on the density of the
packing material
Dead Volume 60 μL to 75 μL
Table 2 lists shipping solvents for Guard-Pak inserts packed with
polymer-based sorbents. Flush the assembled Guard-Pak with a
miscible solvent before use.
Table 2: Shipping Solvents for Polymer-Based Inserts
Sorbent Shipping Solvent
Fast Fruit Juice 10% Methanol/Water
IC-Pak™ Ion-Exclusion 10% Methanol/Water
FAM-Pak
Sugar-Pak™ II 0.1% calcium acetate
IC-Pak Anion Borate buffer with 60% Acetonitrile
Silica-based Guard-Pak inserts are shipped dry and require conditioning
with the organic component of the mobile phase before use.
™
10% Methanol/Water
10% Methanol/Water
v. ORDeRINg INfORmaTION
To order these and other parts and supplies, contact you Waters
Representative.
Table 3: Guard-Pak Inserts and Spare Parts
Guard-Pak Insert Part Number
Nova-Pak C
Delta-Pak C18, 100Å WAT036870
Nova-Pak C
Delta-Pak C18, 300Å WAT036875
Nova-Pak Phenyl WAT020795
Delta-Pak C4, 100Å WAT036860
Nova-Pak Silica WAT020790
Delta-Pak C4, 300Å WAT036865
Nova-Pak CN HP WAT020800
Resolve C
μBondapak C
Resolve C
μBondapak NH
Resolve CN WAT085826
μBondapak Phenyl WAT026745
Resolve Silica WAT085825
μBondapak CN WAT026750
IC-Pak Anion WAT010551
FAM-Pak WAT011818
IC-Pak Ion Exclusion WAT020770
Fast Fruit Juice WAT015207
IC-Pak C M/D WAT044250
Sugar-Pak WAT015209
Puresil
Guard-Pak Holder Kit WAT088141
Guard-Pak In-line Filters, 5/pkg. WAT032472
Guard-Pak O-ring, 1/pkg. WAT072917
Compression screw and Ferrule, 5/pkg. WAT025604
18
8
18
18
8
2
™
Refer to the Waters Chromatography Columns, SPE, Supplies and Waters
Quality Parts Catalog at www.waters.com/chemcat for a complete list of
column accessories and supplies.
WAT015220
WAT035880
WAT085824
WAT088070
WAT026755
WAT026760
WAT044347
Guard-Pak Holder and Inserts 3
[ Care and Use ManUal ]
vI. WaRRaNTy/seRvICe INfORmaTION
Waters Corporation warrants its Guard-Pak products in accordance with the
following terms and conditions:
Waters will replace without cost any holder or insert that fails to perform satisfactorily, if notified within 90 days from your receipt. Any column returned
must have a Return Authorization Number granted by Waters Customer
Service Department. Approval is subject to the following exclusions:
• Physical damage to the holder or insert because of misuse or abuse
• Damage to the holder/insert assembly because of use with incom-
patible solvents or use at incorrect temperatures or pressures.
• Failure or decreased performance as a consequence of normal use
Sales Offices:
Austria and European Export
(Central South Eastern Europe,
CIS and Middle East) 431 877 18 07
Australia 2 9933 1777
Belgium 32 2 726 1000
Brazil 55 11 5094 3788
Canada 800 252 4752
China 8621 6495 6999
CIS/Russia +7 495 3367000
Czech Republic 42 02 617 11384
Denmark 45 46 59 8080
Finland +358 9 5659 6288
France (33) 1 30 48 72 00
Germany 49 6196 400600
Hong Kong 852 29 64 1800
Hungary 36 1 350 5086
India and India Subcontinent
91 80 2 837 1900
Ireland 353 1 448 1500
Italy 39 02 274 211
Japan (81) 3 3471 7191
Korea (82) 2 820 2700
Mexico 5255 5200 1860
The Netherlands +31 (0)76-50 87 200
Norway 47 63 84 60 50
Poland (48) 22 833 4400
Puerto Rico 787 747 8445
Singapore 65 6273 1221
Spain 34 93 600 93 00
Sweden 46 8 555 11500
Switzerland 41 62 889 2030
Taiwan 886 2 2543 1898
©2007 Waters Corporation. Waters, Nova-Pak, Delta-Pak,
FAM-Pak, Guard-Pak, IC-Pak, μBondapak, μPorasil, Resolve,
Sugar-Pak, and The Science of What’s Possible are trademarks of
Waters Corporation.
August 2007 WAT080048 Rev 11 EY-PDF
Guard-Pak Holder and Inserts 4
United Kingdom 44 208 238 6100
Waters Corporation
34 Maple Street
Milford, MA 01757 U.S.A.
T: 1 508 478 2000
F: 1 508 872 1990
www.waters.com
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