Waters Certified Containers User Manual

[ CARE AND USE MANUAL ]

CERTIFIED CONTAINERS KIT

CONTENTS

I. INTRODUCTION

II. THE PURPOSE OF CERTIFIED CONTAINERS

III. HOW TO CARE FOR YOUR CERTIFIED CONTAINER

IV. SOLVENT CONSIDERATIONS

V. STORAGE AND STABILITY

VI. SUMMARY

VII. ORDERING INFORMATION

VIII. REFERENCES

I. INTRODUCTION

The Certified Container Kit provides the LC and LC/MS user the ability

to get reliable results unlike generic or other brands of containers.

These LC containers are (empty) bottles in 250 mL and 1 L sizes. They

are uniquely processed and treated, much like the highly-regarded

Waters low TOC vials.

At <15 ppb TOC, the container is free from any potential organic

contaminants which could result in chromatography ghost peaks.

Just as important as the type and purity of the glass, in addition to

the solvents and mobile phases used, the cleanliness of containers

is paramount for increasing the accuracy of your analytical

measurement. Certified Containers provide Waters instrument users

reliable results unlike generic or other brands of media containers.

These are for Waters, by Waters.

The containers can be used on any Waters LC system, including, but

not limited to UPLC®, LC/UV and LC/MS. These ultra-clean bottles are

Type 1, Class A Borosilicate glass that conform to ASTM E-438 and

meet U.S. Pharmacopoeia specifications for Type 1 Borosilicate Glass.

The Certified Container Kit (p/n 186007088) contains 4 certified

1 L bottles, 3 certified 500 mL bottles with LC caps in standard sizes.

The Low Volume Kit (p/n 186007278) contains 5 certified 250 mL

clear bottles, 1 certified 500 mL clear bottle, and a clean container

cap kit. Each container is uniquely processed, treated, and sealed in a

Mylar bag to prevent particulate contamination during transportation

and storage post cleaning. Mylar was chosen for the bag material as

it does not release phthalates. Phthalates are commonly found in

plasticizers using in plastic bag manufacturing which can be released

during storage with detrimental effects on LC and LC/MS systems. Your

Certified Container Kit has come with a Certificate of Analysis (COA)

indicating it’s Total Organic Carbon (TOC) level when delivered to you.

Certified Containers Kit

[ CARE AND USE MANUAL ]

II. THE PURPOSE OF CERTIFIED CONTAINERS

Certified containers provide Waters instrument users reliable results

unlike generic or other brands of media containers. The containers can

be used on any LC system, including, but not limited to UPLC, LC/UV

and LC/MS. Our unique processing and treatment prevents extraneous

peaks and baseline noise stemming from high TOC, thereby yielding the

cleanest and highest quality reservoir containers in the industry. These

containers provide analytical laboratories time saving solutions that

are easy to use and integrated into work flows ultimately meeting their

needs to simplify operations and increase productivity.

Features:



Certified —Low TOC (<15 ppb)



Certificate of Analysis



Validated Cleaning Process



Waters Branded



Individually Bagged and Boxed

III. HOW TO CARE FOR YOUR CERTIFIED CONTAINER

Be sure to use plugs included in the kit to plug all unused solvent

line holes in the cap liner to maintain cleanliness.

Caution: Do not wash certified containers. Designate one solvent or solvent mix for
one container. The Certified Containers will experience varying use conditions while in
service. During this time, if there is an increase in baseline then it is advised to replace
the Certified Containers with new stock. If baseline increase is not prominent, then it is
advised to use the Certified Containers for 4 to 6 months.

IV. SOLVENT CONSIDERATIONS

Solvent quality will have a significant impact on your performance

and results. Please follow the recommendations listed below for best

results. A more detailed review of these recommendations can be

found in Controlling Contamination in UltraPerformance LC®/MS and

HPLC/MS Systems 715001307, Rev. F at www.waters.com.



Use clean, particle-free solvents



Use ultrapure water



Prevent microbial growth



Degas all solvents



Use miscible solvents

Your sealed Certified Containers have been processed to contain

less than 15 ppb TOC upon receipt. You may notice that the familiar

polypropylene pour ring is absent from these containers. Based

on our deep and wide knowledge of our customer’s work flows,

Waters knows that pour rings can be one of the major sources of

contamination during the use and care of these containers. They have

been removed to give you the best possible product.

You can be confident in the starting point of your bottle. T he quality

of your solvents can impact the TOC level. Take care in choosing

quality solvents to minimize added contamination as described in the

section above. The kit comes with color coded caps which can aid in

segregating solvent types to a particular color code.

It is not necessary to pre-rinse your bottle with solvent but this

behavior will not hinder cleanliness. DO NOT pre-rinse your

Certified Container with solvent. This behavior will not enhance

cleanliness. Just fill the bottle with your designated solvent.

Each bottle contains an enhanced writing square to include your

important solvent information. We recommend you use only one

solvent type for the life of each bottle.

V. STORAGE AND STABILITY

The Certified Containers have been processed, treated, tested, and

sealed to maintain cleanliness. They are stable indefinitely before

opening. Once opened the containers should be used immediately to

avoid degradation and contamination which could compromise the

benefits of the product.

VI. SUMMARY

The glass processing, conditions of bottling and sterilization

processes used by your glass manufacturer could mean the difference

in your analysis and product stability due to ion leaching and pH

changes. Waters Certified containers provided to Waters instrument

users reliable results.

At <15 ppb, the container is free from any potential contaminants

which could result in chromatography ghost peaks.

Certified Containers are will help in your analysis and make your work

easier. Download the LCGC article RP1795EN00 and see how TOC

levels can impact your chromatography, and/or go online and buy the

new kit.

Certified Containers Kit