Waters Beverage Analysis Kit User Manual
[ CARE AND USE MANUAL ]
Beverage Analysis Kit
CONTENTS
I. INTRODUCTION
II. PREPARING THE STANDARDS SOLUTION
III. SETTING UP OR REPLACING MOBILE PHASE
IV. INSTRUMENT SETTINGS
V. S TORAG E
VI. OREDERING INFORMATION
I. INTRODUCTION
This instruction sheet describes the Waters® Beverage Analysis Kit
(P/N 176002534) and how to use it. It has been designed for use
with soft drink formulations containing Acesulfame-K, Saccharin,
Caffeine, Benzoate, Sorbate, and Aspartame.
The Waters Beverage Analysis Kit is made up of four parts:
1. Beverage Analysis Mobile Phase—Four 1-liter bottles
containing a pre-mixed solution of denatured ethanol and
acetate buffer (P/N 186006006). Circulate one liter of mobile
phase through the system for one week, then replace with one
liter of fresh mobile phase.
2. Beverage Analysis Wash Solvent—Four 1-liter bottles containing
a pre-mixed solution of denatured ethanol and water (P/N
186006007). Circulate one liter of wash solvent as the wash on the
system for one week, then replace with one liter of fresh wash solvent.
3. Beverage Analysis Standards—Four 50 mg bottle of
Aspartame powder (P/N 186006010) and Four 100 mL
bottles o f 5 beverage analysis standards (P/N 186006008)
Caffeine 100 mg/L Benzoate 200 mg/L, Sorbate 100 mg/L,
Acesulfame-K 150 mg/L, and Saccharin 100mg/L.
WARNING: Denatured ethanol is toxic. Do not ingest.
[ CARE AND USE MANUAL ]
II. PREPARING THE STANDARDS SOLUTION
Prepare the standards solution by mixing 5 Beverage Analysis
Standard and Aspartame. Prepare fresh standards solution weekly
and store in the refrigerator.
1. Pour the entire contents (100 mL) of 5 Beverage Analysis
Standards (P/N 186006008) into the bottle of aspartame
powder (P/N 186006010).
2. Cap and shake vigorously until the aspartame is dissolved.
Viewing against a dark background helps to determine when
this occurs.
3. Transfer to vials for direct usage. Label as “Beverage Analysis
Standard”. Store remaining unused vials in refrigerator.
Refrigerated Beverage Analysis Standard vials expire after 1
week.
The standards solution prepared by this method contains:
Caffeine 100 mg/L
Benzoate 200 mg/L
Sorbate 100 mg/L
Acesulfame-K 150 mg/L
Saccharin 100mg/L
Aspartame 500 mg/L
7. Once stable, the system should be recalibrated using the Waters
Beverage Analysis Standard.
8. The mobile phase is stable for one week under normal laboratory
conditions.
9. Set the flow rate to 0.5 mL/min.
Figure 1. Chromatogram of the Beverage Analysis Standards Separation
IV. INSTRUMENT SETTINGS
Table 1 shows the settings used to obtain the chromatogram in
Figure 1.
III. SETTING UP OR REPLACING MOBILE PHASE
The Waters Beverage Analysis Mobile Phase can be re-circulated for
one week. Waters recommends that the mobile phase be replaced
with freshly prepared mobile phase using the following procedure:
1. Set the pump flow rate to 0.0 mL/min.
2. Remove the waste line from the solvent reservoir. Place the waste
line into a waste solvent container.
3. Put the solvent line into the bottle containing freshly made
mobile phase.
4. Follow the manufacturer’s instructions for priming and starting
your solvent pump.
5. Set the pump flow to 0.5 mL/min and wait 15 minutes.
(W hen installing the ACQUITY UPLC® BEH Phenyl column
(P/N 186002885), be sure to displace the column packing
solvent and send to waste before recycling).
6. Monitor the baseline for stability. When stable, return the waste
line to the solvent reservoir to recirculate.
Pump ACQUITY UPLC H-Class
Data Acquisition Empower
®
Detection UV @ 214 nm
Sample
Waters Beverage Standard Mixture
from Waters Beverage Analysis Kit
Eluent Waters Beverage Mobile Phase
Column
ACQUITY UPLC BEH Phenyl
2.1 x 100 mm P/N 186002885
Injection Volume 1 µL
Column
Temperature
35 °C
V. S TORAG E
Store Beverage Analysis Mobile Phase at room temperature
indefinitely. Allow Beverage Analysis Standard in direct use vials
to come to room temperature before using. Make fresh standards
solution weekly. Refrigerate the bottle of aspartame.
Footer Title RIGHT
2
Loading...