[ Care and Use ManUal ]
ACQUITY UPLC CSH COLUMNS
Contents
I. GettInG started
a. Column Connectors
b. Column Installation
c. Column Equilibration
d. eCord Installation
e. Initial Column Efficiency Determination
f. VanGuard Pre-Columns
II. CoLUMn Use
a. Sample Preparation
b. pH Range
c. Solvents
d. Pressure
e. Temperature
III. CoLUMn CLeanInG, reGeneratInG and storaGe
a. Cleaning and Regeneration
b. Storage
IV. IntrodUCInG eCord InteLLIGent CHIP teCHnoLoGY
a. Introduction
b. Installation
c. Manufacturing Information
d. Column Use Information
Thank you for choosing a Waters ACQUITY UPLC® CSH™ column.
ACQUITY UPLC CSH columns feature Waters Charged Surface
Hybrid (CSH) Technology which provides excellent peak shape,
high efficiency and loading capacity for basic compounds when
using acidic, low ionic strength mobile phases. T his same particle
™
technology is used in the XSelect
thus enabling seamless transferability between HPLC and UPLC
system platforms. The ACQUIT Y UPLC CSH packing materials were
designed specifically for use with the ACQUITY UPLC systems and
are manufactured in a cGMP, ISO 9001 certified manufacturing
facility using ultra pure reagents. Each batch of ACQUITY UPLC CSH
material is tested chromatographically with acidic, basic and neutral
analytes and the results are held to narrow specification ranges to
assure excellent, reproducible performance. Every column is individu-
ally tested and a Performance Chromatogram and Certificate of Batch
Analysis are provided on the eCord
ACQUITY UPLC CSH columns were designed and tested specifically
for use on ACQUIT Y UPLC systems. ACQUITY UPLC CSH columns will
exhibit maximum chromatographic performance and benefits ONLY
when used on holistically-designed ACQUITY UPLC systems since these
systems and columns were created and designed to operate together.
For these reasons, Waters cannot support the use of ACQUITY UPLC
columns on any system other than an ACQUITY UPLC system.
CSH family of HPLC columns,
™
intelligent chip.
®
V. addItIonaL InForMatIon
a. Tips for Maximizing ACQUITY UPLC CSH Column Lifetimes
b. Recommended Flow Rates and Backpressures for
ACQUITY UPLC CSH Columns
ACQUITY UPLC CSH Columns 1
[ Care and Use ManUal ]
I. GettInG started
Each ACQUITY UPLC CSH column comes with a Certificate of
Analysis and Performance Test Chromatogram embedded within
the eCord intelligent chip. The Certificate of Analysis is specific to
each batch of packing material contained in the ACQUIT Y UPLC CSH
columns and includes the gel batch number, analysis of unbonded
particles, analysis of bonded particles, and chromatographic results
and conditions. The Performance Test Chromatogram is specific to
each individual column and contains such information as: gel batch
number, column serial number, USP plate count, USP tailing factor,
capacity factor, and chromatographic conditions. T hese data should
be stored for future reference.
a. Column Connectors
ACQUITY UPLC systems utilize tubing and connectors which have
been designed to meet stringent tolerance levels and to minimize
extra column volumes.For information on system tubing and
connectors, please refer to the ACQUIT Y UPLC System Operator’s
Guide (Part Number 71500082502).
b. Column Installation
Note: The flow rates given in the procedure below are for typical 2.1 mm i.d. by 50 mm
length 1.7 µm columns. Scale the flow rate up or down accordingly based upon the flow
rate and pressure guide provided in Section V (Additional Information).
1. Purge the pumping system of any buffer-containing mobile phases and
connect the inlet end of the column to the injector outlet.
c. Column Equilibration
ACQUITY UPLC CSH columns are shipped in 100% acetonitrile. It is
important to ensure mobile-phase compatibility before changing to a
different mobile-phase system. Equilibrate the column with a minimum
of 10 column volumes of the mobile phase to be used (refer to Table 1
for a list of column volumes). The column may be considered thermally
equilibrated once a constant backpressure is achieved.
Table 1. Empty Column Volumes in mL (multiply by 10 for flush solvent volumes)
Cloumn Length
(mm)
30 - 0.1 0.2
50 0.04 0.2 0.4
100 0.08 0.4 0.8
150 0.12 0.5 1.0
1.0 mm 2.1 mm 3.0 mm
Internal Diameter
To avoid precipitating mobile-phase buffers on your column or in
your system, flush the column with five column volumes of a water/
organic solvent mixture, using the same or lower solvent content as
in the desired buffered mobile phase. (For example, flush the column
and system with 60% methanol in water prior to introducing 60%
methanol/40% buffer mobile-phase.)
d. eCord Installation
The eCord button should be attached to the side of the column
heater module. The eCord button is magnetized and does not require
specific orientation.
2. Flush column with 100% organic mobile phase (methanol or
acetonitrile) by setting the pump flow rate to 0.1 mL/min and increase
the flow rate to 0.5 mL/min over 5 minutes.
3. When the mobile phase is flowing freely from the column outlet,
stop the flow and attach the column outlet to the detector. This
prevents entry of air into the detection system and gives more rapid
equilibration.
4. Gradually increase the flow rate as described in step 2.
5. Once a steady backpressure and baseline have been achieved, proceed
to the next section.
Note: If mobile-phase additives are present in low concentrations (e.g., ion-pairing
reagents), 100 to 200 column volumes may be required for complete equilibration. In
addition, mobile phases that contain formate (e.g., ammonium formate, formic acid,
etc.) may also require longer initial column equilibration times.
ACQUITY UPLC CSH Columns 2
e. Initial Column Efficiency Determination
1. Perform an efficiency test on the column before using it. T his test may
consist of:
a. an analyte test mixture that is commonly used in your laboratory,
and /or
b. an analyte mixture as found on the “Performance Test
Chromatogram” which accompanied your column.
Note: If b. is performed, the isocratic efficiencies measured in your laboratory may be
less than those given on the Waters “Performance Test Chromatogram”. This is normal.
The Waters isocratic column testing systems have been modified in order to achieve
extremely low system volumes. This presents a more challenging test of how well the
column was packed. This guarantees the highest quality packed column. These special
testing systems have been modified to such an extent that they are not commercially
viable and have limited method flexibility other than isocratic column testing.
[ Care and Use ManUal ]
2. Determine the number of theoretical plates (N) and use this value for
periodic comparisons.
3. Repeat the test at predetermined intervals to track column perfor-
mance over time.
f. VanGuard Pre-Columns
VanGuard™ Pre-columns are 2.1 mm i.d. x 5 mm length guard column
devices designed specifically for use in the ACQUIT Y UPLC systems.
VanGuard Pre-columns are packed with the same chemistries and frits
as our 2.1 mm i.d. ACQUITY UPLC CSH columns. VanGuard Pre-
columns are designed to be attached directly to the inlet side of an
ACQUITY UPLC CSH column.
Note: In order to ensure void-free and leak-free connections, the VanGuard Pre-column is
shipped with the collet and ferrule NOT permanently attached. Care must be taken when
removing the O-ring that holds these two pieces on the pre-column tubing.
™
ACQUITY UPLC® Column
Place wrench here
Ferrule
VanGuard
Collet
Flow
Pre-Column
Place wrench here
Installation Instructions
1. Remove VanGuard Pre-column from box and shipping tube and remove
plastic plug.
2. Orient pre-column so that male end is facing up and carefully remove
rubber O-ring that holds collet and ferrule in place during shipping
(collet and ferrule are not yet permanently attached).
3. Orient ACQUITY UPLC CSH column perpendicular to work surface so
that column inlet is on the bottom (column outlet on top).
4. From below, insert VanGuard Pre-column into ACQUITY UPLC
CSH column inlet and hand-tighten (collet and ferrule are not yet
permanently attached).
5. While pushing the VanGuard Pre-column into the column inlet,
turn assembled column and pre-column 180˚ so that pre-column
is now on top.
6. Tighten with two 5/16” wrenches placed onto ACQUIT Y UPLC CSH
column flats and VanGuard Pre-column hex nut (male end) as shown
above.
7. Tighten 1/4 turn to set collet and ferrule.
8. Check that ferrule is set by loosening connection and inspecting
the ferrule depth. A properly set ferrule depth will resemble other
connections in the ACQUITY UPLC system.
9. Reattach pre-column, apply mobile-phase flow and inspect for leaks.
II. CoLUMn Use
To ensure the continued high performance of ACQUITY UPLC CSH
columns, follow these guidelines:
a. Sample Preparation
1. Sample impurities often contribute to column contamination. One
®
option to avoid this is to use Oasis
®
columns or Sep-Pak
cartridges of the appropriate chemistry to clean
solid-phase extraction cartridges/
up the sample before analysis. For more information,
visit www.waters.com/sampleprep
2. It is preferable to prepare the sample in the operating mobile phase
or a mobile phase that is weaker than the mobile phase for the best
peak shape and sensitivity.
3. If the sample is not dissolved in the mobile phase, ensure that the
sample, solvent and mobile phases are miscible in order to avoid
sample and/or buffer precipitation.
4. Filter sample with 0.2 µm membranes to remove particulates. If the
sample is dissolved in a solvent that contains an organic modifier (e.g.,
acetonitrile, methanol, etc.) ensure that the membrane material does
not dissolve in the solvent. Contact the membrane manufacturer with
solvent compatibility questions. Alternatively, centrifugation for 20
minutes at 8000 rpm, followed by the transfer of the supernatant
liquid to an appropriate vial, could be considered.
b. pH Range
Please see Table 2 below for information on pH range and suggested
operating temperatures.
ACQUITY UPLC CSH Columns 3
[ Care and Use ManUal ]
A listing of commonly used buffers and additives is given in Table 3. Additionally, the column lifetime will vary depending upon the operating
temperature, the type and concentration of buffer used. For example, the use of phosphate buffer at pH 8 in combination with elevated temperatures
will lead to shorter column lifetimes.
Table 2: Recommended pH and Temperature Limits for ACQUITY UPLC CSH Columns
Column Name
ACQUITY UPLC CSH C
18
ACQUITY UPLC CSH Phenyl-
Particle Size
(µm)
1.7 135 185 1-11 80 45 2.3 15
1.7 135 185 1-11 80 45 2.3 14
Pore Diameter
(Å)
Surface Area
(m2)
pH Limits
Hexyl
ACQUITY UPLC CSH Fluoro-
1.7 135 185 1-8 60 45 2.3 10
Phenyl
Note: Working at the extremes of pH, temperature and/or pressure will result in shorter column lifetimes.
Table 3. Buffer Recommendations for Using ACQUITY UPLC CSH Columns up to pH 11
Additive/Buffer pKa Buffer Range Volatility
TFA 0.3 Volatile Yes
Acetic Acid 4.76 Volatile Yes
Formic Acid 3.75 Volatile Yes
Acetate (NH
Formate (NH
COOH) 4.76 3.76 – 5.76 Volatile Yes
4CH2
COOH) 3.75 2.75 – 4.75 Volatile Yes
4
Phosphate 1 2.15 1.15 – 3.15 Non-volatile No
Phosphate 2 7.2 6.20 – 8.20 Non-volatile No
Phosphate 3 12.3 11.3 - 13.3 Non-volatile No
4-Methylmorpholine ~8.4 7.4 – 9.4 Volatile Yes
Ammonia (NH
Ammonium Bicarbonate
4
OH)
9.2
10.3 (HCO
9.2 (NH
+
4
-
)
3
)
8.2 – 10.2
8.2 – 11.3
Volatile
Volatile
Ammonium (Acetate) 9.2 8.2 – 10.2 Volatile Yes
Ammonium (Formate) 9.2 8.2 – 10.2 Volatile Yes
Borate 9.2 8.2 – 10.2 Non-volatile No
CAPSO 9.7 8.7 – 10.7 Non-volatile No
Glycine 2.4, 9.8 8.8 – 10.8 Non-volatile No
1-Methylpiperidine 10.2 9.3 – 11.3 Volatile Yes
CAPS 10.4 9.5 – 11.5 Non-volatile No
Triethylamine
10.7 9.7 – 11.7 Volatile Yes
(as acetate salt)
Pyrrolidine 11.3 10.3 – 12.3 Volatile Yes
Used for
Mass Spec
Yes
Yes
Comments
Ion pair additive, can suppress MS signal, used in the 0.02-0.1% range.
Maximum buffering obtained when used with ammonium acetate salt. Used in 0.1-1.0% range.
Maximum buffering obtained when used with ammonium formate salt. Used in 0.1-1.0% range.
Used in the 1-10 mM range. Note that sodium or potassium salts are not volatile.
Used in the 1-10 mM range. Note that sodium or potassium salts are not volatile.
Traditional low pH buffer, good UV transparency.
Above pH 7, reduce temperature/concentration and use a guard column to maximize lifetime.
Above pH 7, reduce temperature/concentration and use a guard column to maximize lifetime.
Generally used at 10 mM or less.
Used in the 5-10 mM range (for MS work keep source >150 ˚C ). Adjust pH with ammonium
hydroxide or acetic acid. Good buffering capacity at pH 10.
Note: use ammonium bicarbonate (NH4HCO3), not ammonium carbonate ((NH4)2CO3)
Used in the 1-10 mM range.
Used in the 1-10 mM range.
Reduce temperature/concentration and use a guard column to maximize lifetime.
Zwitterionic buffer, compatible with acetonitrile, used in the 1-10 mM range. Low odor.
Zwitterionic buffer, can give longer lifetimes than borate buffer.
Used in the 1-10 mM range.
Zwitterionic buffer, compatible with acetonitrile, used in the 1-10 mM range. Low odor.
Used in the 0.1-1.0% range. Volatile only when titrated with acetic acid (not hydrochloric or
phosphoric). Used as ion-pair for DNA analysis at pH 7-9.
Mild buffer, gives long lifetime.
Temperature Limits
Low pH High pH
Ligand Density
(µmol/m2)
% Carbon
ACQUITY UPLC CSH Columns 4
[ Care and Use ManUal ]
c. Solvents
To maintain maximum column performance, use high quality
chromatography grade solvents. Filter all aqueous buffers prior
to use through a 0.2 µm filter. Solvents containing suspended
particulate materials will generally clog the outside surface of
the inlet distribution frit of the column. This will result in higher
operating pressure and poorer performance. See Section V for more
information.
d. Pressure
ACQUITY UPLC CSH columns can tolerate operating pressures up to
18000 psi (1241 bar or 124 MPa).
Note: Working at the extremes of pressure, pH and/or temperature will result in shorter
column lifetimes.
e. Temperature
Temperatures up to 80 ˚C are recommended for operating ACQUITY
UPLC CSH columns in order to enhance selectivity, lower solvent
viscosity and increase mass transfer rates. When operating at high
pH, lower operating temperatures are recommended for longer
column lifetime. Working at high temperatures (e.g. > 70 °C) may
also result in shorter column lifetimes. See Table 2 above for more
information on suggested operating temperatures and pH ranges.
If the column performance is poor after regenerating and cleaning,
call your local Waters office for additional support.
Table 4. Reversed-Phase Column Cleaning Sequence
Polar Samples Non-polar Samples Proteinaceous Samples
1. isopropanol
1. water
2. tetrahydrofuran
(THF)
3. tetrahydrofuran
(THF)
4. methanol 4. hexane
5. water
6. mobile phase 6. mobile phase
*Use low organic solvent content to avoid precipitating buffers.
** Unless a Hexane Tetrahydrofuran Compatibility Kit (Part Number 205000464) has
been installed, running solvents such as THF or hexane should only be considered when
the column cannot be cleaned by running neat, reversed-phase organic solvents such as
acetonitrile. Reduce flow rate, lower operating temperatures and limit system exposure
to THF and/or hexane.
(or an appropriate isopropanol/
water mixture*)
2. methanol
3. dichloromethane
5. isopropanol
(followed by an appropriate
isopropanol/water mixture*)
Option 1: Inject repeated
aliquots of dimethyl sulfoxide
(DMSO)
Option 2: gradient of 10% to
90% B where:
A = 0.1% trifluoroacetic acid
(TFA) in water
B = 0.1% trifluoroacetic acid
(TFA) in acetonitrile (CH
Option 3: Flush column with 7M
guanidine hydrochloride, or 7M urea
CN)
3
b. Storage
Note: Working at the extremes of temperature, pressure and/or pH will result in shorter
column lifetimes.
III. CoLUMn CLeanInG, reGeneratInG and storaGe
a. Cleaning and Regeneration
Changes in peak shape, peak splitting, shoulders on the peak, shifts
in retention, change in resolution or increasing backpressure may
indicate contamination of the column. Flushing with a neat organic
solvent, taking care not to precipitate buffers, is usually sufficient
to remove the contaminant. If the flushing procedure does not solve
the problem, purge the column using the following cleaning and
regeneration procedures.
Use the cleaning routine that matches the properties of the samples
and/or what you believe is contaminating the column (see Table 4).
Flush columns with 20 column volumes of solvent. Increasing
column temperature increases cleaning efficiency.
ACQUITY UPLC CSH Columns 5
For periods longer than four days at room temperature, store
ACQUITY UPLC CSH columns in 100% acetonitrile. For elevated
temperature applications, store immediately after use in 100%
acetonitrile for the best column lifetime. Do not store columns
in buffered eluents. If the mobile phase contained a buffer salt,
flush ACQUITY UPLC CSH columns with 10 column volumes of
HPLC grade water (see Table 1 for common column volumes) and
replace with 100% acetonitrile for storage. Failure to perform this
intermediate step could result in precipitation of the buffer salt in
the column when 100% acetonitrile is introduced. Completely seal
column to avoid evaporation and drying out of the bed.
Note: If a column has been run with a mobile phase that contains formate (e.g.,
ammonium formate, formic acid, etc.) and is then flushed with 100% acetonitrile,
slightly longer equilibration times may be necessary when the column is re-installed
and run again with a formate-containing mobile phase.
[ Care and Use ManUal ]
IV. IntrodUCInG e Cord InteLLIGent
CHIP teCHnoLoGY
a. Introduction
The eCord intelligent chip will provide the history of a column’s
performance throughout its lifetime. The eCord will be permanently
attached to the column to assure that the column’s performance
history is maintained in the event that the column is moved from
one instrument to another.
Figure 1. Waters eCord Intelligent Chip
Waters eCord intelligent chip
At the time of manufacture, tracking and quality control information
will be downloaded to the eCord. Storing this information on the
chip will eliminate the need for a paper Certificate of Analysis.
Once the user installs the column, the software will automatically
download key parameters into a column history file stored on the
chip. In this manual, we explain how the eCord will provide a solution
for easily tracking the history of the columns, reduce the frustration
of paperwork trails, and give customers the reassurance that a well-
performing column is installed onto their instruments.
b. Installation
Install the column into the column heater. Plug the eCord into the side of
the column heater. Once the eCord is inserted into the column heater the
identification and overall column usage information will be available
allowing the user to access column information on their desktop.
c. Manufacturing Information
Figure 2. eCord Inserted into Side of Column Heater
eCord inserted into
side of column heater
ACQUITY UPLC CSH Columns 6
[ Care and Use ManUal ]
d. Column Use Information
The eCord chip provides the customer with column use data. The column dimensions and serial number. The overall column usage information
includes the total number of samples, total number of injections, total sample sets, date of first injection, date of last injection, maximum
pressure and temperature. The information also details the column history by sample set including date started, sample set name, user name,
system name, number of injections in the sample set, number of samples in the sample set, maximum pressure and temperature in the sample
set and if the column met basic system suitability requirements. Up to 50 sample sets can be stored on the eCord chip.
ACQUITY UPLC CSH Columns 7
[ Care and Use ManUal ]
V. addItIonaL InForMatIon
a. Tips for Maximizing ACQUITY UPLC CSH Column
Lifetimes
1. To maximize ACQUITY UPLC CSH column lifetime, pay close
attention to:
Water quality (including water purification system)
Solvent quality
Mobile-phase preparation, storage and age
Sample, buffer and mobile-phase solubilities
Sample quality and preparation.
2. When problems arise, often only one improper practice must
be changed.
3. Always remember to:
Use in-line filter unit or, preferably, a VanGuard Pre-column.
Discourage bacterial growth by minimizing the use of 100%
aqueous mobile phases where possible.
Change aqueous mobile phase every 24-48 hours (if 100%
aqueous mobile phase use is required).
Old aqueous mobile phases. Remember to rinse bottles
thoroughly and prepare fresh every 24-48 hours.
Using phosphate salt buffer in combination with high ACN
concentrations (e.g., > 70%) due to precipitation.
5. Don’t: assume a “bad” column is the culprit when high backpressure or
split peaks are observed.
Investigate cause of column failure:
Backpressure
Mobile phase(s), bacteria, precipitation and/or samples
Peak splitting
Sample quality
Injection solvent strength.
6. Remember: UPLC flow rates are often much lower and, therefore,
mobile phases last much longer (only prepare what you need or store
excess refrigerated).
7. Mobile-phase related questions to ask:
Am I using 100% aqueous mobile phases? Am I able to add a
small amount of organic modifier to my mobile-phase A?
• Discardold100%aqueousmobilephasesevery24-48hoursto
discourage bacterial growth.
• Add5%-10%organicmodifiertomobilephaseAandadjust
gradient profile.
• Filteraqueousportionsofmobilephasethrough0.2µmfilter.
• Maintainyourwaterpurificationsystemsothatitisingood
working order.
• Onlyuseultrapurewater(18megohm-cm)waterandhighest
quality solvents possible. HPLC grade water is not UPLC grade
water.
• Considersamplepreparation(e.g.,solid-phaseextraction,
filtration, etc).
4. Avoid (where possible):
100% aqueous mobile phases (if possible).
HPLC-grade bottled water.
“Topping off” or adding “new” mobile pha se to “old” mobile phase.
Do I filter my aqueous mobile phases through 0.2 µm filters?
How old is my mobile phase? Do I label the bottle with
preparation date?
Do I “top off” or do I prepare fresh mobile phases every 24-48
hours?
W hat is the quality of my water? Has the quality recently
changed? How is my water purification system working? When
was it last serviced?
Am I working with a pH 7 phosphate buffer (which is VERY
susceptible to bacterial growth)?
8. Sample-related questions to ask:
If I inject neat standards prepared in mobile phase do I observe
these problems?
If I prepare my standards in water and prepare them like samples
(e.g., SPE, filtration, etc.) do I still observe these problems?
Has the quality of my samples changed over time?
ACQUITY UPLC CSH Columns 8
[ Care and Use ManUal ]
b. Recommended Flow Rates and Backpressures for ACQUITY UPLC CSH Columns
1.0 mm i.d. Columns (40 °C)
UPLC Linear Velocity
(mm /se c)
Column Dimensions
1.0 x 50 mm 0.1 4300 0.13 5600 0.17 7400 0.2 8700
1.0 x 100 mm 0.1 8600 0.13 11200 0.17 14600 0.2 17200
1.0 x 150 mm 0.1 12800 0.13 16700 0.17 21800 0.2 25600
UPLC Linear Velocity
(mm /se c)
Column Dimensions
2.1 x 30 mm 0.45 3000 0.60 4100 0.75 5100 0.9 6100
2.1 x 50 mm 0.45 4800 0.60 6400 0.75 8000 0.9 9500
2.1 x 100 mm 0.45 9100 0.60 12100 0.75 15200 0.9 18200
2.1 x 150 mm 0.45 13400 0.60 17900 0.75 22400 0.9 26900
Flow Rate
(mL/min)
Flow Rate
(mL/min)
3 4 5 6
Backpressure
(psi)
Flow Rate
(mL/min)
Backpressure
(psi)
Flow Rate
(mL/min)
Backpressure
(psi)
Flow Rate
(mL/min)
2.1 mm i.d. Columns (40 °C)
3 4 5 6
Backpressure
(psi)
Flow Rate
(mL/min)
Backpressure
(psi)
Flow Rate
(mL/min)
Backpressure
(psi)
Flow Rate
(mL/min)
Backpressure
(psi)
Backpressure
(psi)
3.0 mm i.d. Columns (40 °C)
UPLC Linear Velocity
(mm /se c)
Column Dimensions
3.0 x 30 mm 0.9 3400 1.17 4400 1.53 5800 1.8 6800
3.0 x 50 mm 0.9 5100 1.17 6600 1.53 8700 1.8 10200
3.0 x 100 mm 0.9 9300 1.17 12100 1.53 15900 1.8 18700
3.0 x 150 mm 0.9 13600 1.17 17600 1.53 23100 1.8 27100
Note: 1. ACQUITY UPLC CSH 1.7 µm particle reversed-phase columns
2. ACN/Aqueous gradient, Pmax at ~30% acetonitrile
3. Approximate maximum total system backpressure given
Flow Rate
(mL/min)
3 4 5 6
Backpressure
(psi)
Flow Rate
(mL/min)
Backpressure
(psi)
Flow Rate
(mL/min)
Backpressure
(psi)
Flow Rate
(mL/min)
Backpressure
(psi)
ACQUITY UPLC CSH Columns 9
[ Care and Use ManUal ]
Sales Offices
Austria and European Export
(Central South Eastern Europe, CIS
and Middle East) 43 1 877 18 07
Australia 61 2 9933 1777
Belgium 32 2 726 1000
Brazil 55 11 4134 3788
Canada 1 800 252 4752
China 86 10 5293 6688
Czech Republic 420 2 617 11384
Denmark 45 46 59 8080
Finland 358 9 5659 6288
France 33 1 30 48 72 00
Germany 49 6196 400 600
Hong Kong 852 2964 1800
Hungary 36 1 350 5086
Norway 47 6 384 6050
Poland 48 22 833 4400
Puerto Rico 1 787 747 8445
Russia/CIS 7 495 727 4490/ 290 9737
Singapore 65 6593 7100
Spain 34 93 600 9300
Sweden 46 8 555 115 00
Switzerland 41 56 676 7000
Taiwan 886 2 2501 9928
United Kingdom 44 208 238 6100
All other countries:
Waters Corporation U.S.A.
1 508 478 2000
1 800 252 4752
www.waters.com
India 91 80 2837 1900
Ireland 353 1 448 1500
Italy 39 02 265 0983
Japan 81 3 3471 7191
Korea 82 2 6300 4800
Mexico 52 55 52 00 1860
The Netherlands 31 76 508 7200
©2011 Waters Corporation. Waters, The Science of W hat’s
Possible, ACQUITY, UPLC, ACQUITY U PLC, CSH, ACQUITY
UPLC CSH, XSelect, eCord, VanGuard, Oasis and Sep-Pak are
trademarks of Waters Corporation. All other trademarks are the
property of their respective owners.
October 2011 720003397EN Rev B VW-P DF
Waters Corporation
34 Maple Street
Milford, MA 01757 U.S.A.
T: 1 508 478 2000
F: 1 508 872 1990
www.waters.com
ACQUITY UPLC CSH Columns 10
Loading...