Waters AccQ-Tag Ultra C18 Column User Manual

[ CARE AND USE MANUAL ]

ACCQ•TAG ULTRA C18 CO LUMN

CONTENTS

I. INTRODUCTION

II. GETTING STARTED AND COLUMN USE

III. COLUMN CLEANING AND STORAGE

a. Cleaning

b. Storage

IV. INT RODUCING E CORD INTELLIGENT
CHIP TECHNOLOGY

a. Introduction

b. Installation

c. Manufacturing Information

d. Column Use Information

I. INTRODUCTION

The AccQ•Tag™ Ultra C18, 2.1 x 100mm Column (P/N

®

186003837), is an integral component of Waters UPLC

Acid Analysis Solution. The Solution consists of an integrated

combination of instrumentation, derivatization chemistry, column,

eluents, methods, software, and support. Consequently and when

used as documented, analysts are assured of accurate and precise

amino acid analyses with this detailed complete application solu

tion. The use of the AccQ•Tag Ultra Column without the rest of the

application solution is not supported as an amino acid analysis

method because there are significant risks of inaccurate qualitative

and quantitative analysis.

The AccQ•Tag Ultra Column significantly differs from the

AccQ•Tag 3.9 x 150 mm Column (P/N WAT052885) in both

chemistry composition and column configuration. In addition, the

quality control tests for the AccQ•Tag Ultra Column vs. AccQ•Tag

Column are different, further supporting the fact that these two

column chemistries cannot be used interchangeably.

Note: Please refer to Waters “UPLC Amino Acid Analysis Solution: P/N

720001837EN”, “UPLC Amino Acid Analysis Solution System Guide: P/N

71500129702, and ACQUITY UPLC
Analysis System Guide: USRM134761474 for additional information related
to use of the AccQ•Tag Ultra C18 Column for this defined application solution.

®

H-Class and H-Class Bio Amino Acid

Amino

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[ CARE AND USE MANUAL ]

II. GET TING STARTED AND COLUMN USE

Please refer to the Waters UPLC Amino Acid Analysis Solution

System Guide (P/N 71500129702) for detailed information. This

can be found by searching the literature code on www.waters.com.

III. COLUMN CLEANING AND STORAGE

a. Cleaning

When required, the AccQ•Tag Ultra C18 column can be safely

cleaned with 100% acetonitrile at 0.5 mL/min for 30 minutes.

b. Storage

Please refer to the Waters UPLC Amino Acid Analysis Solution

System Guide (P/N 71500129702) for details on short vs longer

term column storage conditions.

IV. INTRODUCING ECORD INTELLIGENT
CHIP TECHNOLOGY

a. Introduction

At the time of manufacture, tracking and quality control

information will be downloaded to the eCord. This includes the

conditions and results for the Performance Test Chromatogram.

Storing this information on the chip will eliminate the need for a

paper Certificate of Analysis. Once the user installs the column,

the software will automatically download key parameters into a

column history file stored on the chip. In this manual, we explain

how the eCord will provide a solution for easily tracking the history

of the columns, reduce the frustration of paperwork trails, and

give customers the reassurance that a well performing column is

installed onto their instruments.

b. Installation

Install the column into the column heater. Plug the eCord into

the side of the column heater. Once the eCord is inserted into the

column heater, the identification and overall column usage infor

mation will be available in the ACQUITY Console, allowing

the user to access column information on their desktop.

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The eCord™ intelligent chip is a new technology that will provide

the history of a column’s performance throughout its lifetime. The

eCord will be permanently attached to the column to assure that

the column’s performance history is maintained in the event that

the column is moved from one instrument to another.

 eCord Intelligent

Figure 1: Waters eCord Intelligent Chip

eCord Inserted into Side of Column Heater 

Figure 2: Determination of Gradient Delay Volume

2

AccQ•Tag Ultra C18 Column