Veris VIS-NIR Operating Instructions

Operating Instructions
VIS/NIR Spectrophotometer
Table of Contents
Section 1
1-2 Warranty 1-3 Safety
Section 2 2-1 System Overview 2-6 Software Installation Guide
2-11 Configuring Ocean Optics Spectrometer 2-15 Setting port order
2-16 Setting Integration Time Section 3
3-1 Field Operations--Electronics 3-13 Bench top usage 3-15 Test/Example data 3-16 Field OperationsNIRS and EC Mobile Sensor Platform
Section 4
4-1 Maintenance and Lubrication 4-8 NIRS Wear-plate replacement 4-11 Shank Removal 4-14 Case Removal
Section 5
5-1 Troubleshooting 5-6 Procedure #1 Checking Ocean Optic Spec 5-8 Procedure #2 Checking Hamamatsu Spec 5-10 Procedure #3 Shutter Movement 5-12 Procedure #4 Troubleshooting with HyperTerminal 5-16 Procedure #5 Fuse Replacement 5-20 Procedure #6 Checking voltage on Hamamatsu
5-21 Procedure #7 Replacing halogen lamp
5-23 Glossary 5-24 Field Auxiliary output definitions 5-27 Program modifications
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Section 1
MSP with Near Infrared Spectrophotometer
Warranty
Veris Technologies warrants this product to be free of defects in materials and workmanship for a period of one (1) year from the date of delivery to the purchaser. Veris Technologies will repair or replace any product returned to Salina, Kansas, which appears upon inspection to be defective in materials or workmanship. Veris Technologies will have shall have no obligation under this warranty for the cost of labor, down-time, transportation charges, or for the repair or replacement of any product that has been misused, carelessly handled, modified, or altered.
ALL OTHER WARRANTIES OF ANY KIND, WHETHER EXPRESSED OR IMPLIED, INCLUDING BUT NOT LIMITED TO ANY IMPLIED WARRANTY OF MERCHANTABILITY OR OF FITNESS FOR A PARTICULAR PURPOSE AND ALL CLAIMS FOR CONSEQUENTIAL DAMAGES, ARE SPECIFICALLY DISCLAIMED AND EXCLUDED.
Safety
Important! Read the following SAFETY PROCEDURES before operating the Veris MSP:
Read and understand all instructions on safety decals
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• Escaping fluid under pressure can penetrate the skin causing serious injury. Avoid
the hazard by relieving pressure before disconnecting hydraulic lines. Use a piece of paper or cardboard, NOT BODY PARTS, to check for suspected leaks.
• Wear protective gloves and safety glasses or goggles when working with hydraulic and
high-pressure wash systems.
• If an accident occurs, see a doctor immediately. Any fluid injected into the skin must
be surgically removed within a few hours or gangrene may result.
• Pinch point hazard: to prevent injury, stand clear when raising or lowering any part of
the Veris MSP.
• Install all transport locks before transporting or working underneath.
• Detach and store implements in an area where children normally do not play. Secure
implement by using blocks and supports.
• Read Operations Manual before operating machine
• Review safety instructions with operators before operating machine and at least
annually
• Never stand on or use tire as a step
Do not tow the implement on public roads without the optional light package, or without
the proper safety equipment and licensing as required by your State Department of Transportation. Always use safety chain.
• Riders obstruct the operator’s view. They could be struck by foreign objects or
thrown from the machine.
• Never allow children to operate equipment.
To prevent possible electrical shock, or damage to the instrument, do not connect to
any power source greater than twelve (12) volts DC.
• Do not grease or oil implement while it is in operation.
• Disk edges are sharp. Be careful when working in this area.
• Disconnect battery ground cable (-) before servicing or adjusting electrical systems or
before welding on implement.
• Remove buildup of mud, oil or debris.
• Be prepared if a fire starts.
• Keep a first aid kit and fire extinguisher handy.
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Spectrometer case EC
Lamp
Temperature
Motor
GPS
Spare Auxiliary case
USB ports
Section 2
System Overview
Description:
The Veris Spectrophotometer acquires absorbance measurements of soil while being pulled through the field. Alternatively, the unit can be set up in stationary mode in a laboratory to acquire absorbance measurements of samples.
System diagram
Figure 1
Figures 2 and 3
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Figure 4
Shank module #30267
Cable that includes fiber, motor control and lamp power
Garmin GPS # 21221
GPS adaptor cable #30727
Note: This adaptor cable is required in order for compatibility with the provided Garmin GPS.
Shank bench top stand #33204
GPS serial adaptor cable #35482 This will connect the serial port of a GPS to the GPS port on the auxiliary case, for using an alternate GPS to the Garmin. External power is required for the GPS to function.
Figure 5
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Figure 6
Figure 7
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Figure 8
External reference blocks and reference block holder which are used to calibrate instrument during the system check.
External reference block holder
Soil temperature sensor #30276
5-meter USB cables #30281
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Figure 9
Figure 10
Figure 11
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Figure 12
USB repeaters #32891
These are to be used only when the regular 5-meter long USB cables are not long enough.
15-amp power supply #34669
This will power the instrument in a bench top setting.
Power cable #33158
This is used to power the instrument from a vehicle (for field use).
Note: Only use 15-amp fuse
Power cable for 15-amp power supply
Figure 13
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Figure 14
Figure 15
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Wearplate assembly #31934 For serial #s 1001 thru 1005
Wearplate assembly #35488 For serial #s 1006 and beyond
Figures 16 & 17
Note: For computers outside the United States of America, please make the
following change to the computer’s regional settings before installing the Veris
Spectrophotometer Software.
Step 1: Open control panel and double click on Regional and Language Options Step 2: Click on Customize, the following screen will appear. The Decimal symbol
needs to be a “.” while the Digit grouping symbol needs to be a “,”. The will ensure
proper operation of the software. Once the changes have been made click OK and proceed with installation.
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Veris Spectrophotometer Software Installation
The Veris Spectrophotometer Software installer will guide the user through installation of the Veris operating software and the necessary drivers needed to run the spectrometers. Note:
Do not plug in USB cables for cases at this time.
Keep the default directory and click next
Veris Spectrophotometer Software V1.77 and up will run on either Windows XP, Vista or 732 bit operating systems only
Figure 18
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Figure 19
Figure 20
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Now the ocean optics drivers will be installed. Select language and continue.
Leave the default directory and click next
Select next to complete install
Figure 21
Figure 22
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Figure 23
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Figure 24
At this point it is OK to restart the computer
Select Install the software automatically, then click Next to complete the hardware installation.
Next the Hamamatsu Minispectrometer installation guide will appear. Click next two process through the installer
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Figure 25 Once the restart is complete, plug in the USB cables for the auxiliary and spectrometer
cases MS Windows may then bring up the following screens about the Ocean Optics
spectrometer and the Hamamatsu spectrometer.
Figure 26
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Windows will automatically find the correct drivers for the Ocean Optics USB4000.
For the Hamamatsu installation select Install the software automatically then Next to complete installation.
If this screen appears asking for the driver version to be installed always use the specu1a.inf, then select Next.
Figure 27
After completing the Ocean Optics MS Windows installer, the Hamamatsu installer may appear. Note: These installers may appear in any order.
Figure 28
Figure 29
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The master_veris_standards file is specific to your external references and is provided by Veris.
Installation of Calibration Files
This must be complete for the software to be operational Copy the master_veris_standards.txt file from the calibration CD into the C:\Veris
directory
Figure 30
Figure 31
Figure 32
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Configuring Ocean Optics Spectrometer
Step 1: Open OOIBase32 Software (only necessary for Veris Spectrophotometer Software V1.76 and below) Start Ocean Optics software (Start programs Ocean Optics OOIBase32)
a. If software asks for a default spectrometer file click cancel
Figure 33 If it asks for a spectrometer configuration click, OK
Figure 34
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If software asks for a default spectrometer file click Cancel
Figure 35
Step 2: Open Configuration
Click on Spectrometer in the toolbar at the top and then select Configure
Figure 36 On the configuration screen select the A/D Interface tab
Figure 37
Note: Under Spectrometer Type if USB4000/HR4000 is not available select S4000
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Under serial # only one spectrometer number should be present. If no serial number is present close out of the software, unplug the USB cable and reinsert the USB cable, wait to see if windows recognizes a new device, then proceed to the open the Ocean Optics software and try again.
Click OK and a spectrum will be present on the screen.
Figure 38 Example of spectrum that should be shown on screen (when light is on at shank).
Step 3: Save Configuration
Figure 39
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Click on Spectrometer then Save Configuration As.
Figure 40 The Save Spectrometer Configuration should automatically fill in the file name with the
serial number. Click Save.
Figure 41 Choose yes to make the spectrometer configuration the default settings.
The spectrometer has now been configured.
Figure 42 Exit the software.
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Setting port order
Auxiliary case ports
Spectrometer case ports
The software could increment the number of the ports by one depending on the computer. For example, the device manager shows ports 20,21,22,23,9 but the software shows 21,22,23,24,10. Just use the incremented numbers if this situation occurs.
- Check Device Manager for port order (Start settings control panel system hardware tab device manager double click ports)
- Ports will be listed in order, as they should be set in software.
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Figure 43 In this example the order to set the ports would be as follows: (see Figure 5.) Different
computers will have the ports in different orders; however if the software is used on one computer only, the ports will never change. The ports will always be in the order of spare, GPS, temp, control, and spectrometer. For future reference, make note of the com port order your computer is using.
Spare: COM20 GPS: COM11 Temp: COM12 Control: COM13 Spectrometer: COM8
The last port (COM9) will not be used.
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Example of ref spectra shown in system check and acquisition with correct integration time set.
Ocean Optics Integration time Hamamatsu Integration time
Setting Integration Time:
The integration time is the amount of time the spectrometers collect the spectral data for averaging. It is important to have this set correctly because too much light will saturate the spectrometer, while too little light won’t allow the full range of the detector to be used. The integration time needs to be set before acquiring spectral data. This is
especially important for users switching between probe and shank modules as the optics are different and the amount of light seen by each device varies.
Step 1: Open the Veris Spectrophotometer Software and start the system check. After the first Dark and Reference measurements are stored check the ref spectra display (example shown below). The two peaks shown should be around 40000.00 counts. The first peak is controlled by the Ocean Optics (oo) int time, while the second peak is controlled by the Hamamatsu (ham) int time. If peaks are too high, then lower the integration time. If peaks are too low then raise the integration time.
Step 2: Once it has been determined which way to move the integration time, exit the software and open the acq_settings.txt file located in the C:\Veris directory.
0.010 12.000 5.000 8.000 0.150 0.150 0.300 0.150 2.000 Example of data stored in acq_settings.txt
The second value represents the ham integration time, while the third value represents the oo integration time. Raise or lower these values then save the file, open the Veris Spectrophotometer Software, run a dark and reference measurement, and then check these values again to see how the peaks changed.
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Folder icon to select working directory. A field folder must be selected before any steps can be completed. Note: Each field or site must have its own folder.
Once the desired directory is found, click on current folder.
BEFORE STARTING:
1. Before taking any data let system run for 20 minutes to allow the spectrometers to stabilize.
2. Connect spectrometers to PC.
3. Open Veris software
4. The port order must be set (see Setting Port Order). This needs to be done the first time the software is run on a machine, after that the port order will be stored for future use. Port terminology may be labeled COM or ASRL. Both are acceptable if port number is correct.
Press EXIT or F12 when ready to quit the software; this will ensure the port order will be saved.
Section 3
Field Operations: Electronics
Operating software – Software that will guide the user through the field usage steps. This will need to be completed every time the software is started. Open Veris operating software:
Figure 1
Figure 2
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Indicator light will be yellow or green
Number of times each ER has been sampled
1) System Check – This step calibrates the spectra data to known standards to ensure data integrity. This is accomplished by A) manually placing four external reference standards against the window and collecting spectra measurements for each standard, then B) transforming the data to a master set of data provided by Veris.
A) After the Dark and Reference cycle has completed and the ref check light has turned green, the system is ready for the external references. Before running the external references make sure the window on the shank and the window on the references are clean. References can be checked in any order and as many times as necessary. When program state reads REST, the ref check light is green, and controller is in neutral (N), place the correct external reference under shank and then press the corresponding external reference button. If the External Reference (ER) failure light is yellow, check to make sure the correct external reference was placed under the shank and that the correct external reference button was pressed, then run the external reference again. When ER light turns green or yellow, proceed to next reference. Once each external reference has been run at least once, press end or F12. This will exit this program and initiate the create transform program, which will take the data just acquired and compare it to the master data to create a current transform. If the transform is created successfully, then that transform will be applied to all spectra data acquired during the acquisition.
Placing the reference holder beneath shank ER held securely beneath shank Figures 3 & 4
Place the ER securely onto the shank wearplate. The dowel pins on the ER fit into four allen-head screws on the wearplate. This aligns the ER in the correct position, and magnets hold the ER firmly in place. The reference material goes towards the front of the shank and lines up directly underneath the sapphire window on the wearplate. The allen-head screws can get packed with soil after the system has had soil contact. A flat­head screwdriver can be used to remove the soil so the ER can be put in place.
Figure 5
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External Reference buttons
Failure light Number of times
each reference has been ran, since system check was opened.
Manually stores dark/reference data. This should be done if there is a failed reference check. This could be caused by the shank steps not being within specification, or one of the spectrometers is not running. Try to clear out the reference by pressing F2. If problem persists shut down system and restart.
Auxiliary data can be scrolled through by the up and down arrows on the left of the indicator. Data corresponds to chart listed on the right of indicator. Note: a reading of –1.50 indicates failure of that component or communication problem. See Glossary for Auxiliary data information table.
Ref check graph: This should be close to
a straight line except for the edges and the center. The reference check light will indicate if this is an acceptable reference check.
Root mean squared error of current external reference.
By default the RMSE should be within .10 for references 1,2,& 4 and within .15 for reference 3.
Reference check light is green for good references and red for a failed reference. If a reference fails, press F2 to store a new dark and reference.
Ref check light
Graph that compares current external reference to master reference. These should correlate to one another if system check has been completed before.
A yellow ER light indicates possible problem. Regard as a good reference unless transform step fails.
Shank steps should be 5.2 cm within +­.03 cm. If steps are not within this range then press F2 to store another dark/ref.
Figure 6
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B) Transform step.
Raw master and slave data (external reference). The master is black and the slave is grey.
Master and slave data with transform applied master is black and slave is grey.
Root mean squared error for current transform.
Maximum allowed RMSE for a good transform.
Compares the offset of the previous transform the current transform
Indicates when transform is complete. READY TO
MOVE TO NEXT STEP DATA ACQUISITION.
This compares the slope of the previous transform to the current transform
Previous transform will be a straight line the first time system is ran.
External reference data that was just taken.
Master data file, which will be used to transform slave (master file provided by Veris)
Indicates if transform is acceptable.
Figure 7
Master data file is provided by Veris.
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Once the transform is complete press close window or F12. If the transform was successful, the software will inform the user and continue on to acquisition. If the transform failed, the program will enter the system check again so the references can be ran once more. If the transform fails again, either there is a system problem (spectrometer malfunction, window is dirty, or references not taken properly) or the tolerance for the maximum allowed RMSE could be set too low; see program modifications to change this.
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Before mapping complete the following:
Start system at least 20 minutes before starting to map to ensure the spectrometers
have stabilized
Make sure auxiliary sensor data is coming in (see Glossary for auxiliary sensor data
definition). Make sure all auxiliary lights are green. Complete all steps above. Make sure Reference Check (Ref Check) light is green, and
GPS data are being received. Set depth so that EC coulters penetrate approximately 1.5" Make sure the implement frame is level with the ground surface Pull forward with shank in ground. The absorbance should not rise more than 0.1.
2) Data Acquisition--electronics – The collection of spectral and auxiliary data in the field. Data is streamed to a file in a compact format.
Pressing LOG button or ESC key stores spectrum data as absorbance and auxiliary data, which includes EC data, until LOG button or ESC key has been pressed again. No data can be logged if there is no GPS signal or there is a bad ref check. No data, including EC data, will be stored unless it program is in LOG mode. Whenever shank is raised out of the soil, press LOG button or ESC key to stop logging, re-press LOG button or ESC key to re-start logging when shank is in soil.
System will automatically collect a dark and reference every 10 minutesthe next time the LOG button or ESC key is pressed after a 10-minute period. Before re-starting at the end of each round, check to make sure system isn’t collecting a dark and reference.
If an extra dark and reference is desired, pressing F2 or Dark & Ref stores a new reference and dark spectrum. Pressing F4 or sample will store a reference, dark, then transformed spectrum data as absorbance into the acquisition_samples file located in the field directory.
When all data have been acquired press F12 or End to return to the main interface. Soil EC is recorded simultaneously with NIR data, provided the unit is equipped with soil
EC coulter module, and cables are connected properly. When EC is being collected, EC values will appear in the shallow and deep EC windows, as shown in Figure 10. Note: unit must be in Log mode for EC to be recorded. Also, see Maintenance section for information on testing EC system output.
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Ref check light This green light indicates a good reference
These indicate the temperatures and the humidity of the system. If they are not within range (see specifications) the indicator light will be red, if this occurs the system should be shut down.
Dark spectrum that was stored when program state was in dark mode
Reference spectrum that was stored when program state was in reference mode.
Ref check compares the previous reference data to current reference. As in system check, this should be close to a straight line except for the edges and the center. A bad reference is indicated by the ref check light at the top of the screen. If this situation occurs, press F2 to store a new dark and reference.
Pressing LOG or ESC stores spectrum data as absorbance and auxiliary data, which includes EC data, until LOG or ESC has been hit again. No data can be logged if there is no GPS signal or there is a bad ref check. Use LOG to store data while mapping a field. No data, including EC data, will be stored unless it program is in LOG mode.
Auxiliary data indicator
Auxiliary data can be scrolled through by pressing the up or down arrow to the left of the indicator. The auxiliary data corresponds to the chart listed on the right of the indicator.
Figure 8
EC indicators
Log and GPS lightsmust be green for data to be recorded
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As long as the acquisition software is closed properly using F12, all data is saved and the acquisition can be opened at a later time with new data being appended to the data taken previously.
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