UVP White-UV Transilluminators User Manual

White/UV Transilluminators

Instruction Manual

UVP, LLC Ultra-Violet Products Ltd.
2066 W 11th Street, Upland, CA 91786 Unit 1,Trinity Hall Farm Estate,
Tel: [phone] Nuffield Road Cambridge CB4 1TG UK
Fax: [phone] Tel: [phone] / Fax: [phone]

Web site: www.uvp.com

81-0286-01 Rev C

White/UV Transilluminators Page 2

UV Blocking Cover is

surface

Control knob for selection of
Power switch

Long life filters: 20 x

Introduction

The UVP Transilluminator offers the researcher uniform and intense sources of ultraviolet light
(radiation) and white light in one unit. UV and white light 20x20cm filter areas side by side.

The special UV design emits high intensity excitation UV wavelength for back-illumination of
transparent fluorescent materials. The UV back-illumination provides a highly sensitive method to
detect double-stranded nucleic acids that have been labeled with fluorescent dyes such as
ethidium bromide or acridine orange. Single stranded nucleic acids may be detected, but with
excitation wavelength sensitive for nucleic acid visualization.

White light is used to illuminate coommassie blue, silver stain and protein gels.
The transilluminator is uniquely designed with increased UV intensity and uniformity, instant on

capabilities, no lamp flicker and reduced electrical consumption.
NOTE: Though the manual refers to the midrange UV waveband as 302nm, others refer to this

region as 300nm or 312nm. The spectral output of all these regions is the same.
A Word of Caution: UV Transilluminators are powerful sources of UV radiation that will cause

damage to unprotected eyes and skin. Before operating any unit, be sure all personnel in the area
are properly protected. If not using the transilluminator with an imaging system darkroom, a UV
Blocking Cover should be attached to the transilluminator. Even though this cover blocks the
ultraviolet radiation emitted by the unit, UV Blocking Eyewear should be worn as well.

The White/UV Transilluminator features:

hinged and adjustable to
varying heights for access
to the transilluminator

20cm UV and white light
areas side by side

UV or white light