UVP White-UV Transilluminators User Manual

White/UV Transilluminators

Instruction Manual

UVP, LLC Ultra-Violet Products Ltd.
2066 W 11th Street, Upland, CA 91786 Unit 1,Trinity Hall Farm Estate,
Tel: [phone] Nuffield Road Cambridge CB4 1TG UK
Fax: [phone] Tel: [phone] / Fax: [phone]

Web site: www.uvp.com

81-0286-01 Rev C

White/UV Transilluminators Page 2

UV Blocking Cover is

surface

Control knob for selection of
Power switch

Long life filters: 20 x

Introduction

The UVP Transilluminator offers the researcher uniform and intense sources of ultraviolet light
(radiation) and white light in one unit. UV and white light 20x20cm filter areas side by side.

The special UV design emits high intensity excitation UV wavelength for back-illumination of
transparent fluorescent materials. The UV back-illumination provides a highly sensitive method to
detect double-stranded nucleic acids that have been labeled with fluorescent dyes such as
ethidium bromide or acridine orange. Single stranded nucleic acids may be detected, but with
excitation wavelength sensitive for nucleic acid visualization.

White light is used to illuminate coommassie blue, silver stain and protein gels.
The transilluminator is uniquely designed with increased UV intensity and uniformity, instant on

capabilities, no lamp flicker and reduced electrical consumption.
NOTE: Though the manual refers to the midrange UV waveband as 302nm, others refer to this

region as 300nm or 312nm. The spectral output of all these regions is the same.
A Word of Caution: UV Transilluminators are powerful sources of UV radiation that will cause

damage to unprotected eyes and skin. Before operating any unit, be sure all personnel in the area
are properly protected. If not using the transilluminator with an imaging system darkroom, a UV
Blocking Cover should be attached to the transilluminator. Even though this cover blocks the
ultraviolet radiation emitted by the unit, UV Blocking Eyewear should be worn as well.

The White/UV Transilluminator features:

hinged and adjustable to
varying heights for access
to the transilluminator

20cm UV and white light
areas side by side

UV or white light

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When the UV Blocking Cover is not being
used, UV light may escape through the

Safety Plug

Specifications

White/UV Transilluminators

White/UV Transilluminator models are designed with white light and one or two UV
wavelengths. Units are equipped with a high efficiency electronic ballast.
Dimensions: 13.25D x 19.13W x 5.64H in. (337 x 486 x 143mm)

Model Part Number Volt/Hz Wavelength Filter Size No. of Tubes /Watts

TLW-20 95-0415-01 100-115/60 365nm 20 x 20cm 4 x 8W
95-0415-02 230/50 White 20 x 20cm 2 x 8W

TMW-20 95-0415-04 100-115/60 302nm 15 x 15cm 4 x 8W
95-0415-05 230/50 White 20 x 20cm 2 x 8W

LMW-20 95-0418-01 100-115/60 302/365nm 20 x 20cm 4 x 8W
95-0418-02 230/50 White 20 x 20cm 2 x 8W

Transilluminator Operation

Safety Precautions

holes dedicated to accepting the bracket
pins of the UV Blocking Cover.

 Remove the black safety plugs from

their package

 Insert the safety plugs through the

holes as shown.

Setting Up the Transilluminator

 Place the transilluminator on a level work surface. Be sure that an air space exists around the

bottom of the work surface. This space allows for the proper air circulation through the unit.

 Plug the female end of the power cord into the transilluminator. For 230 volt models, or those

requiring special power cord connectors, ensure that the proper configuration of male
connector or plug has been properly connected to the power cord.

 Plug the male end of the power cord into a properly grounded electrical outlet. The proper

voltage of the transilluminator is found on the product information label. If using the
transilluminator with an imaging system, a jumper cable is required for connecting to the
darkroom. Refer to the imaging system documentation for additional instructions.

 The transilluminator is equipped with a UV Blocking Cover. Remove the brown protective

paper from the cover. Insert the bracket pins on the cover into the holes on the front of the
transilluminator. The cover is adjustable to varying angles for access to the filter surface.

White/UV Transilluminators Page 4

NOTE: Do not operate the unit without securing the cover. If the cover is missing, a UV
Blocking Faceshield must be worn to avoid UV exposure to the skin. UV Blocking Eyewear
should be worn even with the cover in place to avoid accidental UV exposure.

Using the Transilluminator

Place gel/sample on the filter area. It is recommended that researchers place the gels on a Gel­Tray to protect the filter surface from cuts and scratches. It is recommended that gloves be worn to
prevent skin contact with gel and staining agents.

 The transilluminator can be turned on with the wavelength selector knob poin tin g in any

position. Press the ON/OFF switch to ON. The tubes within the unit will begin glowing beneath
the filter.

 Dial the knob to the appropriate wavelength setting. After viewing the sample, turn the

transilluminator off.

Maintenance/Repair/Technical Assistance

Cleaning and Care of the Transilluminator

Clean unit surface with a damp soft cloth or sponge. Never use abrasive cleaners (can damage
the filter surface). To protect the filter glass and minimize moisture and liquids on the glass, it is
recommended that you use a UV transmitting Gel-Tray. Refer to the Replacement Parts for
ordering information.

Replacing Tubes in the Transilluminator

 Disconnect the transilluminator for the electrical supply.
 Remove the filter cover: Use a Phillips head screwdriver to remove the four screws on the

sides of the unit. Lift the filter cover off the unit.

 Remove the reflectors on the left and right side. Slide the reflectors up out of the unit.
 Remove the tube: Carefully rotate the tube and slide out of the socket. Replace with a new

tube by sliding the tube into the socket and rotating into place. Insert the reflectors back into
place and reattach the filter cover.

Replacement Parts/Accessories

For replacement parts or components not shown here, please call UVP Customer Service or place
of purchase. Please have the transilluminator model number available when you call.

Replacement Part Description Part Number

Filter Assembly (LMW-20, TMW-20) 38-0189-05
Filter Assembly (TLW-20) 38-0189-08
Cover, UV blocking 19-0112-01
Tube, 8 Watt, 302nm midrange UV (FL8E) 34-0042-01 (Qty. 4 Required)
Tube, 8 Watt, 365nm longwave UV (F8T5/BL) 34-0006-01 (Qty. 4 Required)
Tube, 8 Watt, cool white (T8T5/CW) 34-0056-01 (Qty. 2 Required)
Ballast, electronic universal voltage 42-0068-04 (115V)
42-0068-06 (230V)
Fuse, 2 amp, 250V, Slo-Blo (all voltages) 56-0002-01 (Qty. 2 Required)
White/UV Selector Switch 53-0196-02

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Accessories Description Part Number

Gel-Cutter 85-0002-01
Gel-Scooper 85-0006-01
Gel-Tray, UV Transmitting, Lg. 16.5”W x 10.5”D (42 x 27cm) 85-0005-01
Gel-Tray, High Performance UV Transmitting (25 x 26cm) 38-0296-03
Gel-Ruler, UV Fluorescing 85-0003-01
Spectacles, UV Blocking 98-0002-01
Goggles, UV Blocking 98-0002-02
Faceshield, UV Blocking 98-0002-04

Technical Support

UVP offers technical support for all of its products. If you have any questions about the product’s
use or, operation, please contact UVP’s offices at the following locations.

If you are in North America, South America, If you are in Europe, Africa, the Middle East,
East Asia or Australia: Western Asia:

 Call [phone] or [phone]  Call [phone] Customer
Customer Service regular business regular business days, between 9 am
days, between 7 am and 5 pm PST and 5:30 pm

 E-Mail: [e-mail]  E-Mail: [e-mail]

 Fax Customer Service: [phone]  Fax Customer Service: [phone]

 Write to : UVP, L LC  Write to : Ultra-Violet Products Ltd.

2066 W. 11th Street Unit 1, Trinity Hall Farm Est., Nuffield Rd.

Upland, CA 91786 USA Cambridge CB4 1TG UK

Note: A Returned Goods Authorization (RGA) number must be obtained from UVP Customer

Service before returning any product.

Warranty

UVP, LLC warrants its Ultraviolet Transilluminators to be free of detects in materials and workmanship
for a period of two (2) years from date of purchase. The foregoing warranty of UVP shall be of no force
and effect if buyer has modified or damaged the product. Tubes and filters are warranted for 90 days.

All warranties or merchantability and fitness for any purpose and all other warranties, expressed or
implied, except those expressly set forth herein, are deemed waived and excluded.

UVP’s duty under the warranty is limited to replacement and/or repair of the defective part at the option
of UVP, LLC. UVP shall not be liable for any expenses or damages incurred by the purchaser except as
expressly set forth herein, and in no event shall UVP be liable for any special, incidental or
consequential damages of any kind. This warranty does not supersede any statutory rights that may be
available in certain countries.