UVP VisiDoc-It Imaging System User Manual

VisiDoc-It with Stand

VisiDoc-It with Hood

VisiDoc-It

™ Imaging System

Installation and User Instructions

UVP, LLC Ultra-Violet Products Ltd.

2066 W. 11th Street Unit 1, Trinity Hall Farm Estate
Upland, CA 91786 Nuffield Road, Cambridge CB4 1TG UK
Phone: [phone] Phone: [phone]
Fax: [phone] Fax: [phone]

Web Site: www.uvp.com

81-0309-01 Rev C

VisiDoc-It Imaging System 2

Transilluminator (check the

8” LCD Touch Screen

USB Drive

System Processor

System Introduction

The VisiDoc-It Imaging System is designed for researchers who already have a transilluminator in the lab.
Configurations include a compact hood or camera stand. The compact hood fits on top of UVP’s High
Performance and Benchtop Transilluminators. The VisiDoc-It System provides simple documentatio n of
fluorescent and non-fluoresce nt gels, me mbranes, blots, film plates and assays with the ability to save
images to a USB storage device for later quantitative analysis.

VisiDoc-It Components

Refer to the packing slip and pictured components for parts included with the system.

VisiDoc-It with Hood

Camera and Lens Kit

Hood (with handles) fits over
the filter area on the
transilluminator

packing slip to determine if
the system includes a
transilluminator)

EtBr Filter is mounted in the
top of the hood

VisiDoc-It Imaging System 3

Transilluminator with UV

Camera and Lens Kit

8” LCD Touch Screen

USB Drive

System Processor

VisiDoc-It with Stand

Camera Stand with X/Y
Movement

EtBr Filter is mounted under
the Lens

Blocking Cover (check the
packing slip to determine if
the system includes a
transilluminator)

VisiDoc-It Imaging System 4

Camera

The VisiDoc-It includes the FluorCam 210 Camera

making any adjustments to the camera setting s.

Lens

Processor

Ethidium Bromide Filter (EtBr )

The Ethidium Bromide (EtBr) Filter (50mm sq.) UV

System Specifications

with a resolution of 640 x 480 (VGA) and a USB 2.0
PC interface. All camera settings are factory pre-set
for optimum performance when viewing gels, films,
or membranes under low light level conditions. UVP
Technical Support should be contacted before

The 8-48 mm zoom lens is fitted with a close-up
diopter and step-up ring. The camera can then be
held in a fixed position. The diopter is for focusing
on objects at the focus length of the VisiDoc-It
system.



Internal Storage: 75GB or mor e

 USB 2.0 ports: 1 front, 4 in the back
 USB storage device capacity: 8GB (minimum)
 Software: TS software

blocking bandpass interference filter blocks UV and
IR radiation emitted from the transilluminator. The
filter is placed in the filter tray below the camera
assembly. The filter allows visualization of
fluorophores from 580-630nm, targeting the EtBr
emission which peaks at 605nm. The EtBr filter can
be substituted for other specific fluorophore filters or
removed when imaging non-fluorescent media
(protein gels, colony plates, etc.) in order to produce
brighter images.

Hood Enclosure or Camera Stand for the VisiDoc-It System

The compact hood enclosure is manufactured of lightweight plastic for portability. Handles are
positioned on two sides of the hood to make it easy to move the hood for access to the transilluminator
surface. The camera with a zoom lens is mounted on top of the hood or camera stand.

VisiDoc-It Imaging System 5

UV/White Converter Plate

Transilluminator

For VisiDoc-It systems, transilluminators are optional equipment. The VisiDoc-It System can
accommodate UVP’s Benchtop and FirstLight transilluminator models. The VisiDoc-It (with stand
design) can also accommodate the High Performance UV Transilluminators (25-watt) and White/UV
Transilluminators (8-watt). UVP offers a variety of transilluminators, from the benchtop models with 8­watt, single wavelength and single intensity to multiple wavelengths and high/low intensities, or the
highly uniform FirstLight transilluminator.

LCD Touch Screen

The display is an 8-inch VGA color touch screen connected to the darkroom cabinet with tilt adjustment.
The touch screen allows the user to preview, snap, save and print images, as well as select certain
preference options, without the need of an external mouse or keyboard.

A stylus pen is included for increased pointer control for selecting software options on the touch screen.

Focus Target

The focus target fluoresces when placed on a transilluminator or when exposed to overhead UV. The
Target provides sharp, fluorescent images to aid in adju sti ng the focus of the camera.

Removable USB Stick

The removable USB stick has 8GB memory (minimum) included with the system which connects to the
system allowing transfer of images.

Optional Equipment

Refer to the ordering infor mat i on s ect ion of this man ual for
optional equipment part numbers.

Thermal Printer

The thermal printer provides archive quality, 256 grayscale
prints, and five optional cost-effective print sizes.

Converter Plates

The UV/White Converter Plate allows imaging of non­fluorescent stained media with an ultraviolet transilluminator.
The converter plate is specially coated to convert the 302nm
UV output to white light, rather than using a separate white
light box.

The Visi-Blue Converter Plate converts UV to 460-470nm
designed for use with blue excitation samples and SYBR
green, SYPRO orange and GFP stains.

Transilluminators

If the system does not include a transilluminator (check the packing slip), contact UVP for ordering
information or go to
high performance FirstLight Transi llu mi nator.

www.uvp.com. Available transilluminators include the compact 8-watt models to the

Printer