UVP VisiDoc-It Imaging System User Manual

VisiDoc-It with Stand

VisiDoc-It with Hood

VisiDoc-It

™ Imaging System

Installation and User Instructions

UVP, LLC Ultra-Violet Products Ltd.

2066 W. 11th Street Unit 1, Trinity Hall Farm Estate
Upland, CA 91786 Nuffield Road, Cambridge CB4 1TG UK
Phone: [phone] Phone: [phone]
Fax: [phone] Fax: [phone]

Web Site: www.uvp.com

81-0309-01 Rev C

VisiDoc-It Imaging System 2

Transilluminator (check the

8” LCD Touch Screen

USB Drive

System Processor

System Introduction

The VisiDoc-It Imaging System is designed for researchers who already have a transilluminator in the lab.
Configurations include a compact hood or camera stand. The compact hood fits on top of UVP’s High
Performance and Benchtop Transilluminators. The VisiDoc-It System provides simple documentatio n of
fluorescent and non-fluoresce nt gels, me mbranes, blots, film plates and assays with the ability to save
images to a USB storage device for later quantitative analysis.

VisiDoc-It Components

Refer to the packing slip and pictured components for parts included with the system.

VisiDoc-It with Hood

Camera and Lens Kit

Hood (with handles) fits over
the filter area on the
transilluminator

packing slip to determine if
the system includes a
transilluminator)

EtBr Filter is mounted in the
top of the hood

VisiDoc-It Imaging System 3

Transilluminator with UV

Camera and Lens Kit

8” LCD Touch Screen

USB Drive

System Processor

VisiDoc-It with Stand

Camera Stand with X/Y
Movement

EtBr Filter is mounted under
the Lens

Blocking Cover (check the
packing slip to determine if
the system includes a
transilluminator)

VisiDoc-It Imaging System 4

Camera

The VisiDoc-It includes the FluorCam 210 Camera

making any adjustments to the camera setting s.

Lens

Processor

Ethidium Bromide Filter (EtBr )

The Ethidium Bromide (EtBr) Filter (50mm sq.) UV

System Specifications

with a resolution of 640 x 480 (VGA) and a USB 2.0
PC interface. All camera settings are factory pre-set
for optimum performance when viewing gels, films,
or membranes under low light level conditions. UVP
Technical Support should be contacted before

The 8-48 mm zoom lens is fitted with a close-up
diopter and step-up ring. The camera can then be
held in a fixed position. The diopter is for focusing
on objects at the focus length of the VisiDoc-It
system.



Internal Storage: 75GB or mor e

 USB 2.0 ports: 1 front, 4 in the back
 USB storage device capacity: 8GB (minimum)
 Software: TS software

blocking bandpass interference filter blocks UV and
IR radiation emitted from the transilluminator. The
filter is placed in the filter tray below the camera
assembly. The filter allows visualization of
fluorophores from 580-630nm, targeting the EtBr
emission which peaks at 605nm. The EtBr filter can
be substituted for other specific fluorophore filters or
removed when imaging non-fluorescent media
(protein gels, colony plates, etc.) in order to produce
brighter images.

Hood Enclosure or Camera Stand for the VisiDoc-It System

The compact hood enclosure is manufactured of lightweight plastic for portability. Handles are
positioned on two sides of the hood to make it easy to move the hood for access to the transilluminator
surface. The camera with a zoom lens is mounted on top of the hood or camera stand.

VisiDoc-It Imaging System 5

UV/White Converter Plate

Transilluminator

For VisiDoc-It systems, transilluminators are optional equipment. The VisiDoc-It System can
accommodate UVP’s Benchtop and FirstLight transilluminator models. The VisiDoc-It (with stand
design) can also accommodate the High Performance UV Transilluminators (25-watt) and White/UV
Transilluminators (8-watt). UVP offers a variety of transilluminators, from the benchtop models with 8­watt, single wavelength and single intensity to multiple wavelengths and high/low intensities, or the
highly uniform FirstLight transilluminator.

LCD Touch Screen

The display is an 8-inch VGA color touch screen connected to the darkroom cabinet with tilt adjustment.
The touch screen allows the user to preview, snap, save and print images, as well as select certain
preference options, without the need of an external mouse or keyboard.

A stylus pen is included for increased pointer control for selecting software options on the touch screen.

Focus Target

The focus target fluoresces when placed on a transilluminator or when exposed to overhead UV. The
Target provides sharp, fluorescent images to aid in adju sti ng the focus of the camera.

Removable USB Stick

The removable USB stick has 8GB memory (minimum) included with the system which connects to the
system allowing transfer of images.

Optional Equipment

Refer to the ordering infor mat i on s ect ion of this man ual for
optional equipment part numbers.

Thermal Printer

The thermal printer provides archive quality, 256 grayscale
prints, and five optional cost-effective print sizes.

Converter Plates

The UV/White Converter Plate allows imaging of non­fluorescent stained media with an ultraviolet transilluminator.
The converter plate is specially coated to convert the 302nm
UV output to white light, rather than using a separate white
light box.

The Visi-Blue Converter Plate converts UV to 460-470nm
designed for use with blue excitation samples and SYBR
green, SYPRO orange and GFP stains.

Transilluminators

If the system does not include a transilluminator (check the packing slip), contact UVP for ordering
information or go to
high performance FirstLight Transi llu mi nator.

www.uvp.com. Available transilluminators include the compact 8-watt models to the

Printer

VisiDoc-It Imaging System 6

The camera and zoom lens are assembled at the

Step up ring

Diopter

Setup Instructions

Components

 Camera with zoom lens, bracket, filter, cables
 VisiDoc-It hood or stand
 USB storage device, 8GB (minimum)
 Transilluminator (may be ordered separately)

WARNING: DO NOT ATTEMPT TO CONNECT ANY WIRING WHILE THE
EQUIPMENT IS CONNECTED TO ANY POWER SUPPLY

CAUTION:

Do not install the system in places with high moisture, dust, or high temperatures. Do not use any oil or
petroleum based cleaner for the cabinet. Use only mild soap or detergent solution for cleaning. Ensure that
the system is turned OFF during cleaning. Keep the equipment away from motors or other large magnetic
equipment.

Camera Setup

In the VisiDoc-It Hood

UVP factory.



Remove the cap from the lens.



Attach the step up ring and diopter to the
lens. The step up ring and diopter will only fit
one way.



Using the four thumb screws provided,
secure the bracket to the base.

 Slide the camera and lens assembly into the

camera bracket. Slide the lens through the
center hole in the gasket. The gasket forms
a seal around the lens.

VisiDoc-It Imaging System 7



processor power

USB ports

Insert and tighten the knob/washer in the
central hole in the camera to secure the
camera to the bracket.



Plug the camera cable into the top the
camera and the other end into a USB port on
back of the processor.

Connection for the

 Connect the power cord from the

transilluminator to a surge protected power
outlet.

 Connect the main power cord from the back

of the processor to a surge protected power
outlet.

Printer Setup

The thermal printer is an optional accessory. Proceed to the next section if a printer was not included with
the shipment.

Stylus and Holder

The screen is touch sensitive. If desired, use the stylus included with the system to operate the software
controls. For easy access to the stylus when operating the system, remove the tape from the back of the
holder and adhere it to the VisiDoc-It. Slide the stylus into the holder when not in use.

VisiDoc-It Imaging System 8



Main power

Using the System

Powering Up the System



Power up the system by pushing once on the MAIN
POWER button located on the right side of the

processor.

 Turn on the transilluminator.



To power down the system, push the main power
button.

Adjusting the Screen Angle

To adjust the angle of the screen, loosen the
knobs on each side of the monitor. Twist each
knob counter-clockwise.

 Tilt the screen to the desired angle.
 Turn each knob clockwise to tighten.

Operating the TS Software

The system will go through the boot-up process. When ready, the LCD will display the TS software screen
similar to the one below:

VisiDoc-It Imaging System 9

To shut down the TS software, touch the X on the bottom left corner of the screen.
To turn the system off, press the MAIN POWER button or power down from the Windows icon on the bottom

right corner of the screen.
Refer to Touch Screen Interface in this manual for detailed instr uct ions on using the software.

Using the Transilluminator

To use the transilluminator, turn on the transilluminator power on the front of the unit.
NOTE: When the transilluminator is illuminated, ensure that the UV blocking cover is in position to prevent

any exposure to UV.

Image Focusing

Prior to capturing images, prepare the image focus capabilities of the system:

 Turn on the transilluminator and place the F luor es cent Foc us Target on the transilluminator surface.
 While watching the LCD screen, rotate the lens f-stop adjustment so that the ima ge is brig ht enough to

see on the screen.

 Rotate the lens focus adjustment on the lens. Adjust this so that the image appears in clear focus on the

LCD monitor. Note: Once the proper zoom range is set, the lens will have to be refocused when
zoomed in completely.

 Rotate the zoom lens adjustm ent so that the image is as large as possible. Readjust the focus ring on

the lens, making the image clear. Adjust the zoom so that the object of interest is within the frame on
the LCD monitor.

VisiDoc-It Imaging System 10

Touch Screen Interface

Setting User Preferences on the TS Software

Before capturing images, set up the user preferences by touching the PREF (preferences) button on
the main screen. Select from the following tabs:

 Capture
 Postprocessing
 Saving

Capture Tab

Active Camera: The name of the attached ca mera w ill be shown.
Bit Depth: Select the bit depth. 16 bpp (bits per pixel) is shown for the Gel HR Camera.
Display Time Stamp: Turn the time stamp on or off.

Postprocessing Tab

Auto Rotate: To rotate the image, click the OFF button and then the down arrow. Rotate options are:

0, 90, 180 or 270 degrees.
Auto Invert Image: Click ON to automatically invert the colors within the image.

VisiDoc-It Imaging System 11

Saving Tab

NOTE: If the auto save function is enabled, the image will automatically save when the SNAP button is

pressed. If the auto save function is not enabled, press the SAVE button to save an image. A pop up
screen will allow the user to save to the USB Drive or a different location with Prompt for Location
function.

Save Images To: Select the location where images are to be saved. White highlight indicates which
button is activated. The USB DRIVE will save the file to the USB drive if one is plugged in. If one is not
plugged in, the user will be prompted to select a different location. PROMPT FOR LOCATION will ask
the user to select a location when the SAVE button is pre ss ed.

Save Format: Click the down arrow to select the file format. Save images as TIFF (16-bit), JPEG (8-bit)
or GIF (8-bit) file formats.

Auto Save To: Click the white box to active the auto save function. To change the folder to a different
location, click in the folder field and a “Browse for Folder” window will pop up. Select a folder or make a
new folder. Click OK to close the window. The selected folder will display in the auto save to box.

Auto Print: Select from Never, Always, or After Save

NOTE: These options will be reflected after capturing an image with the SNAP button, and not in LIVE

mode.
Touch the OK button to save all preferences and go back to the main screen, or the Cancel button to go

back to the main screen without saving changes.

VisiDoc-It Imaging System 12

Tool/Function

Procedure

Adjust exposure

2. Adjust the exposure time by first selecting the time interval. Touch on the

3. To view overexposed pixels, touch the EXP WARN button. Overex posed

4. To add a date stamp to the image, touch the TIME STAMP button. This will

TIME STAMP

EXP WARN

PREF

Windows Icon
OFF (X) – Shuts

PRINT - Calls up
SAVE

SNAP

LIVE
Decrease

Status Bar

Min / Sec / mSec

Increase integration

Identifying the Touch Screen Functions and Tools

Using the software touch screen allows convenient selection of image capture, save, print and other
functions of the system. Callouts are provided below to briefly describe the buttons.

Preview an image
before snapping it

Capture an image

Store the captured
image

the Print dialog
box to choose print
options

down the TS
software

integration time

Using the Functions and Tools

Adds a date stamp
to the image as
follows:
UVP####mm/dd/yy

Indicates mode of
operation and
exposure time

In Live Mode, the
system will show
overexposed pixels
in yellow
(overexposure) or
red (high
overexposure)

Navigate to the
Preferences screen

Touch icon to go
access the Windows
desktop

Display time interval

time

1. To see a preview of the image before snapping it, touch the LIVE button.

time

LIVE mode will be indicated in the status bar.

corresponding bars, Minutes, Seconds, milliSeconds, from left to right,
respectively. These bars will be animated in LIVE/SNAP mode to show
progress as time elapses. Decrease or increase the time by touching the
minus or plus button respectively.

pixels will be identified with a yellow or red color, depending on the how
saturated they are.

add UVP#####mm/dd/yy at the bottom right corner of the image. This
information is NOT burned into the image unt il the image is s av ed.

VisiDoc-It Imaging System 13

5. To snap (capture) the image, touch the SNAP button. NOTE: When using

6. Touch the SAVE button to save the image to the location specified in the

7. Print the image (if a printer is installed) by touching the PRINT button and

Status bar

8. Status bar indicat es the mod e s of operat ion : IDLE, LIVE or CAPTURE.

Managing Files

X

Min. Sec. MSec

the SNAP button, if the Auto Save function is enabled, the image will
automatically be saved.

Preferences. The file name is assigned automatically by the software as

UVP#####mmddyyyy.*

NOTE: Once an image is saved, the user cannot open the image with the

software. TIFF images can be opened by VisionWorks or comparable software
(contact UVP Tech Support for recommendations). JPEG and GIF files can be
opened with many Microsoft or comparable programs.

clicking OK at the print dialog box.

modes

Minutes, seconds, milliseconds bars display time progress. Each field

(Min, Sec, Msec) displays the current exposure time and the start of the
current exposure being taken. The large portion of each field indicates the
current exposure status. The smaller portion to the right indicates the
current exposure setting.

9. To go Microsoft Windows, touch the WINDOWS ICON located at the
bottom right corner of the screen. To turn off the system, shut down from
Windows or push the MAIN POWER button.

To close the software, click the X in the bottom left corner of the screen.

Find and View Images

The TS System allows the user to save images in several locations. The location
is selected in the PREF screen. The system has a built-in hard drive with at least
25 GB of storage space that can be used.

To save images in the USB drive, make sure a USB drive is plugged in. If one is
not plugged in, the TS software will automatically prompt the user for a location.

Viewing Images

It is not possible to view saved images on the software screen. To view images
captured and saved from the TS system, different software is required.

To view 8-bit JPEG or GIF images, exit to Windows and open Windows Image
Viewer or other comparable software program. To exit the TS software interface
and go to Microsoft Windows, touch the WINDOW ICON located at the bottom
right corner.

To view 16-bit TIFF images, o pen Visio nWorksLS or comparable program
(contact UVP Tech Support for recommendations). VisionWorksLS may be
included with the system. To check, go to Start > All Programs > UVP >
VisionWorks or look for the VisionWorks icon on the desktop.

VisiDoc-It Imaging System 14

Network

Connecting to a Network

The TS System has built-in networkability. While it is fairly simple to connect the

Connectivity

device to the network, it is highly recommend to obtain assistance from your
network administrator.

Follow the Microsoft or local standard network protocols for network
configuration. To exit the TS software interface and go to Microsoft Windows,
touch the WINDOWS ICON located at the bottom right corner of the main
screen.

Install Drivers or Additional Software

Installing Drivers

or Additional

Software

In the event that additional drivers or VisionWorksLS software need to be
installed on the system, exit the TS software interface and go to Microsoft
Windows by pressing the WINDOWS ICON button located at the bottom right
corner of the main screen.

To install drivers or additional software, copy to a USB storage device, open
Windows Explorer in TS and run the desired program. The system can accept
USB as a method of software installation.

VisiDoc-It Imaging System 15

Service Procedures

Return Procedure

A Returned Goods Authorization (RGA) number must be obtained from UVP Customer Service before
returning any product.

Replacement Parts and Accessories

To order accessories or replacement parts for the VisiDoc-It S y ste m, con t a ct UV P’s o ff i ce s.

Part Description Part Number

Filter, Ethidium Bromide, 50mm Square 38-0220-01
Filter, SYBR Green, 50mm Square 38-0219-01
Filter, SYBR Gold, 50mm Square 38-0221-01

White Light Converter Plate, 21x26cm 38-0191-01
White Light Converter Plate, 25x26cm 38-0191-04
Visi-Blue Converter Plate, 21x26cm 38-0200-01
Visi-Blue Converter Plate, 25x26cm 38-0200-04

Gel-Cutter 85-0002-01
Gel-Ruler 85-0003-01
Gel-Scooper 85-0006-01
Gel-Tray, small 85-0007-01
Gel-Sentry DNA Preparation Plate 97-0076-01
Fluorescent Standard Step Tablet 33-0014-02

Doc-It®LS Analysis Software 97-0185-02
Spectacles, UV Blocking (UVC-303) 98-0002-01

Goggles, UV Blocking (UVC-503) 98-0002-02
Faceshield, UV Blocking (UVC-803) 98-0002-04

Troubleshooting

No Power to the Darkroom

Recheck main power cord connections to the darkroom .
Check fuses, located at the back of the unit, near the power port. You will need a flat-head screwdriver. Turn

the cap and the fuse holder will “pop-out”. Inspect the thin wire within the glass fuse to see if there is a break
in the wire. If so, replace fuse(s). If fuses are blown repeatedly, contact UVP Technical Support Department
for additional troubleshooting.

No Image on the LCD Screen

To power up the system, push once on the button located on the left side of the unit.

Touch Screen not Accurate

If touching or clicking on the buttons becomes inaccurate, you may need to recalibrate your touch screen.
Please follow all instructions in the touch screen calibration instructions included in your TS software CD.

VisiDoc-It Imaging System 16

If you are in North America, S outh

If you are in Europe, Africa, the

Call [phone] or (909) 946-

Call [phone], and ask for
E-mail your message to:

E-mail your message to: [e-mail]

Fax Customer Service, and send it to

Fax Customer Service, and send it to:

Write to: UVP, LLC 2066 W. 11th

Write to: Ultra-Violet Products Ltd

Error Messages Pop Up on the Screen

Messages appear on the screen that might be related to the TS software interface, or Microsoft Windows. If
the message is related to Microsoft Windows, such as a reminder to activate or update your copy of
Windows, please contact your system administrator for assistance.

If an error message appears repetitively, and your system administrator does not recognize it as a Microsoft
Windows error, contact UVP Technical Support for further assistance.

Technical Support

UVP offers technical support on all of its products. If you have any questions about the product’s use,
operation or repair, please contact our offices at the locations below. Or go to UVP’s web site and click the
Tech Support > BioImaging Systems.

Warranty

UVP's products are guaranteed to be free of defects in materials, workmanship and manuf actu re for two (2)
years from date of purchase. Consumable and di sposab le par t s includ ing, but not l imited to t ubes and filters,
are guaranteed to be free from defects in manufact ure and ma ter ials for ninety (90) days from date of
purchase. Transilluminators carry a tw o (2) year war ranty from date of purchas e. If equipment failure or
malfunction occurs during the warranty per iod, UV P shall ex amine the inoperat iv e equipment and hav e the
option of repairing or replacing any part(s) w hich, in the judgm ent of U VP , wer e originally defectiv e or became
so under conditions of normal usage and service.

No warranty shall apply to this instrument, or part thereof, that has been subject to accident, n egligence,
alteration, abuse or misuse by the end-user. Moreover, UVP makes no warranties whatsoev er w ith r espect to
parts not supplied by UVP or that have been installe d, used an d/or serv iced other than in strict compliance with
instructions appearing in this manual.

In no event shall UVP be responsible to the end-user for any incidental or consequential damages, whether
foreseeable or not, including but not limited to property damage, inability to use equipment, lost business,
lost profits, or inconvenience arising out of or connected with the use of instruments produced by UVP. Nor
is UVP liable or responsible for any personal injuries occurring as a result of the use, installation and/or
servicing of equipment.

This warranty does not supersede any statutory r ights that may be av ailable in certain countries.

America, East Asia or Australia:

3197, and ask for Customer Service

during regular business days, between
7:00 am and 5:00 pm, PST.

[e-mail]

[phone]

Street, Upland, CA 91786 USA

VisiDoc-It is a trademark of UVP , LLC. Doc-It is a registered trademark of UVP, LLC.

Middle East of Western Asia:

Customer Service during regular

business days between 9:00 am and
5:30 pm.

[phone]

Unit 1, Trinity Hall Farm Estate, Nuffield

Road, Cambridge CB4 1TG UK