BioDoc-It
™ and VisiDoc-It™
Imaging Systems
Installation and User Instructions
UVP, LLC Ultra-Violet Products Ltd.
2066 W. 11th Street Unit 1, Trinity Hall Farm Estate
Upland, CA 91786 Nuffield Road, Cambridge CB4 1TG UK
Phone: (800) 452-6788 / (909) 946-3197 Phone: +44(0)1223-420022
Fax: (909) 946-3597 Fax: +44(0)1223-420561
BioDoc-It Imaging System VisiDoc-It Imaging Systems
81-0301-01 Rev A
Web Site: www.uvp.com
BioDoc-It and VisiDoc-It Imaging Systems 2
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System Introduction
BioDoc-It™ Imaging System
The BioDoc-It Imaging System enables simple documentation of fluorescent and non-flu oresce nt gels,
membranes, blots, film plates and assays with the ability to save images to a USB storage device for later
quantitative analysis or enhancement for publication.
Images are saved in 8-bit TIFF or JPEG formats allowing the images to be read by most PC or MAC
programs. The BioDoc-It System is a cost effective solution for archiving quality images in a compact
standalone package. No computer is required.
VisiDoc-It™ Imaging System
The VisiDoc-It Imaging System is designed for researchers who already have a transilluminator in the lab.
Configurations include a compact hood or camera stand. The compact hood fits on top of UVP’s High
Performance and Benchtop Transilluminators. The VisiDoc-It System is similar to the BioDoc-It in its
functions for simple documentation of fluorescent and non-fluorescent gels, membranes, blots, film plat es
and assays with the ability to save images to the USB storage device for later quantitative analysis.
System Components
BioDoc-It Components
Refer to the packing slip and pictured components for parts included with the system.
8” LCD Touch Screen
with Tilt Capability
USB Drive
Wide Access
Door
View
Port
FluorCam 210 Camera and
Zoom Lens
Filter Tray (located under the
camera) with EtBr Filter
Main System Power Switch
White Light and Darkroom
Power Switches
UV to White Light Converter
order separatel
Plate
Transilluminator
Side Access Doors (on
select models)
BioDoc-It and VisiDoc-It Imaging Systems 3
Internal Storage: 75GB or more
USB 2.0 ports: 1 front, 4 in the back
USB storage device capacity: 1GB (minimum)
Software: TS software
VisiDoc-It Components
Refer to the packing slip and pictured components for parts included with the system.
VisiDoc-It™ with Hood
FluorCam 210 Camera with
Lens Kit
Hood (with handles) fits over
the filter area on the
transilluminator
Transilluminator (check the
packing slip to determine if
the system includes a
transilluminator)
EtBr Filter is Mounted in the
Top of the Hood
8” LCD Touch Screen
USB Drive System Processor
BioDoc-It and VisiDoc-It Imaging Systems 4
VisiDoc-It™ with Stand
Camera Stand with X/Y
Movement
FluorCam 210 Camera with
Lens Kit
EtBr Filter is Mounted under
the Lens
8” LCD Touch Screen
Transilluminator with UV
Blocking Cover (check the
packing slip to determine if
the system includes a
transilluminator)
USB Drive System Processor
BioDoc-It and VisiDoc-It Imaging Systems 5
System Specifications
Camera
FluorCam 210 Camera with a resolution of 640 x
480 (VGA) with a USB 2.0 PC interface. All camera
settings are factory pre-set for optimum performance
when viewing gels, films, or membranes under low
light level conditions. UVP Technical Support should
be contacted before making any adjustments to the
camera settings.
Lens
The 5.7-34.2mm zoom lens is fitted with a close-up
diopter and step-up ring. The camera can then be
held in a fixed position. The diopter is for focusing
on objects at the focus length of the BioDoc-It and
VisiDoc-It system.
Processor
Internal Storage: 75GB or more
USB 2.0 ports: 1 front, 4 in the back
USB storage device capacity: 1GB (minimum)
Software: TS software
Ethidium Bromide Filter (EtBr)
The Ethidium Bromide (EtBr) Filter (50mm sq.) UV
blocking band pass interference filter blocks UV and
IR radiation emitted from the transilluminator. The
filter is placed in the filter tray below the camera
assembly. The filter allows visualization of
fluorophores from 580-630nm, targeting the EtBr
emission which peaks at 605nm. The EtBr filter can
be substituted for other specific fluorophore filters or
removed when imaging non-fluorescent media
(protein gels, colony plates, etc.) in order to produce
brighter images.
Darkroom for the BioDoc-It System
The darkroom is light tight for optimum imaging conditions. The darkroom features:
Gel viewer window
Overhead white light
Wide access doors with UV safety switch
Side doors (darkroom may or may not include side doors)
BioDoc-It and VisiDoc-It Imaging Systems 6
Hood Enclosure or Camera Stand for the VisiDoc-It System
The compact hood enclosure is manufactured of lightweight plastic for portabilit y. Handles are
positioned on two sides of the hood to make it easy to move the hood for access to the transilluminator
surface. The camera with a zoom lens is mounted on top of the hood or camera stand.
Transilluminator
A transilluminator is included with the BioDoc-It system. For VisiDoc-It systems, transilluminators are
optional equipment. The BioDoc-It and VisiDoc-It System can accommodate UVP’s Benchtop and
FirstLight transilluminator models. The VisiDoc-It (with stand design) can also accommodate the High
Performance UV Transilluminators (25-watt) and White/UV Transilluminators (8-watt). UVP offers a
variety of transilluminators, from the benchtop models with 8-watt, single wavelength and single
intensity to multiple wavelengths and high/low intensities, or the high-end FirstLight trans illuminator.
Note: For UV protection, there is a 10-minute timer installed in the BioDoc-It system. The timer shuts
off the transilluminator after 10 minutes of operation. A switch on the front of the darkroom allows the
user to reset the timer. When the darkroom door is opened, this automatically resets the timer.
LCD Touch Screen
The display is an 8-inch VGA color touch screen connected to the darkroom cabinet with tilt adjustment.
The touch screen allows the user to preview, snap, save and print images, as well as select certain
preference options, without the need of an external mouse or keyboard.
A stylus pen is included for increased pointer control for selecting software options on the touch screen.
Focus Target
The focus target fluoresces when placed on a transilluminator or when exposed to overhead UV. The
target provides sharp, fluorescent images to aid in adjusting the focus of the camera.
Removable USB Stick
The removable USB stick has 1 GB memory (minimum) included with the system which connects to the
system allowing transfer of images.
BioDoc-It and VisiDoc-It Imaging Systems 7
Optional Equipment
Refer to the ordering information section of this manual for
optional equipment part numbers.
Thermal Printer
The thermal printer provides archive quality, 256 grayscale
prints, and five optional cost-effective print sizes.
Converter Plates
The UV/White Converter Plate allows imaging of nonfluorescent stained media with an ultraviolet transilluminator.
The converter plate is specially coated to convert the 302nm
UV output to white light, rather than using a separate white
light box.
The Visi-Blue Converter Plate converts UV to 460-470nm
designed for use with blue excitation samples and SYBR
green, SYPRO orange and GFP stains.
Transilluminators
If the system does not include a transilluminator (check the packing slip), contact UVP for ordering
information or go to UVP’s web site at www.uvp.com
from UVP. These units include compact 8-watt models to our high performance FirstLight
Transilluminator.
. A large selection of transilluminators is available
Printer
UV/White Converter Plate
BioDoc-It and VisiDoc-It Imaging Systems 8
Set-up Instructions
Components
Camera with zoom lens, bracket, filter, cables
BioDoc-It darkroom or VisiDoc-It hood or stand
USB storage device, 1 GB (minimum)
Transilluminator (may be ordered separately)
WARNING: DO NOT ATTEMPT TO CONNECT ANY WIRING WHILE THE
EQUIPMENT IS CONNECTED TO ANY POWER SUPPLY
CAUTION
Do not install the system in places with high moisture, dust, or high temperature. Do not use any oil or
petroleum based cleaner for the cabinet. Use only mild soap or detergent solution for cleaning. Ensure that
the system is turned OFF during cleaning. Keep the equipment away from motors or other large magnetic
equipment apparatus.
Installing the Filter
Systems include a 50mm sq EtBr filter. The filter is pre-installed in the VisiDoc-It hood at the UVP factory.
To install the 50mm sq Ethidium Bromide (EtBr) filter in the BioDoc-It:
Carefully remove the filter from the protective
plastic case, holding the filter at the edges to
prevent fingerprints.
The filter tray is located under the camera bracket
on the right side top of the darkroom. Slide the
tray open.
Place the filter inside the filter tray so that the text
on the edge of the filter is positioned so the letters
are right side up when facing you.
Additional filters are available. Refer to Accessories for ordering information.
BioDoc-It and VisiDoc-It Imaging Systems 9
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Camera Set-up
In the BioDoc-It
The camera and zoom lens are assembled at the
UVP factory.
Remove the cap from the lens.
Attach the step up ring and diopter to the
lens. The step up ring and diopter will only
fit one way.
Using the four thumb screws provided,
secure the bracket to the base.
Step up rin
Diopte
Slide the camera and lens assembly into the
camera bracket. Slide the lens through the
center hole in the gasket. The gasket forms
a seal around the lens.
Insert and tighten the knob/washer in the
central hole in the camera to secure the
camera to the bracket.
Camera cable
Camera and lens
assembl
Thumb screws
secure the bracket
to the base
BioDoc-It and VisiDoc-It Imaging Systems 10
Plug the camera cable into the top the
camera and the other end into a UBS port on
back of the darkroom.
Connection for the
darkroom power
Connect the jumper cord from the darkroom
to the transilluminator.
Connect the main power cord from the back
of the darkroom to a surge protector power
outlet.
Connection for
jumper cord to the
transilluminator
USB port
BioDoc-It and VisiDoc-It Imaging Systems 11
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In the VisiDoc-It Hood
The camera and zoom lens are assembled at the
UVP factory.
Remove the cap from the lens.
Attach the step up ring and diopter to the
lens. The step up ring and diopter will only
fit one way.
Using the four thumb screws provided,
secure the bracket to the base.
Slide the camera and lens assembly into the
camera bracket. Slide the lens through the
center hole in the gasket. The gasket forms
a seal around the lens.
Step up rin
Diopte
Insert and tighten the knob/washer in the
central hole in the camera to secure the
camera to the bracket.
Thumb screws
secures the
bracket to the
base
BioDoc-It and VisiDoc-It Imaging Systems 12
Plug the camera cable into the top the
camera and the other end into a UBS port on
back of the processor.
Connect the power cord from the
transilluminator to a surge protector power
outlet.
Connection for the
darkroom power
Connect the main power cord from the back
of the processor to a surge protector power
outlet.
USB port
Printer Set-up
The thermal printer is an optional accessory. Proceed to the next section if a printer was not included with
the shipment.
BioDoc-It and VisiDoc-It Imaging Systems 13
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Stylus and Holder
The screen is touch sensitive. Use the stylus included with the system to operate the software controls. For
easy access to the stylus when operating the system, remove the tape from the back of the holder and
adhere it to the BioDoc-It / VisDoc-It. Slide the stylus into the holder.
Using the System
Powering Up the System
In the BioDoc-It
Power up the system by pushing once on the MAIN
POWER button located on the right side of the unit.
All internal components will now have power.
Turn on the transilluminator and darkroom power
switches.
To power down the system, push the main power
button.
Main powe
In the VisiDoc-It
Power up the system by pushing once on the MAIN
POWER button located on the right side of the
processor.
Turn on the transilluminator.
To power down the system, push the main power
button.
Main powe
BioDoc-It and VisiDoc-It Imaging Systems 14
Adjusting the Screen Angle
To adjust the angle of the screen, loosen
the knobs on each side of the monitor.
Twist each knob counter-clockwise (note
that you will actually be turning each knob
in the opposite direction from each other).
Tilt the screen to the desired angle.
Turn each knob clockwise to tighten.
Operating the TS Software
The system will go through the boot-up process. When ready, the LCD will display the TS software screen
similar to the one below:
To shut down the TS software, touch the X on the bottom left corner of the screen.
To turn the system off, press the MAIN POWER button or power down from the Windows icon on the bottom
right corner of the screen.
Refer to the TS Software User Manual for further instructions on using the software.
BioDoc-It and VisiDoc-It Imaging Systems 15
Using the Transilluminator
In the BioDoc-It
Once the BioDoc-It darkroom power is turned on, power is supplied to all components including the jumper
power cable that supplies power to the transilluminator. To use the transilluminator, turn the transillumin ator
power on.
For UV protection, there is a 10-minute timer installed in the system. The timer shuts off the transilluminator
after 10 minutes of operation. A switch on the front of the darkroom allows the user to reset the timer. When
darkroom door is opened, this automatically resets the timer. When the timer runs out, press the timer again.
This prevents the transilluminator from being on for too long and prevents damage to gels.
Refer to the Transilluminator Manual for additional instructions on using the transilluminator.
In the VisiDoc-It
To use the transilluminator, turn on the transilluminator power on the front of the unit.
NOTE: When the transilluminator is illuminated, ensure that the UV blocking cover is in position to preven t
any exposure to UV.
Using the Gel Viewing Window in the BioDoc-It
The Gel Viewer has a pressure-sensitive clasp. Press firmly to open the viewer. The glass is UV blocking
while providing a clear view to the transilluminator surface for visibility of samples without opening the door.
Image Focusing
Prior to capturing any images, prepare the image focus capabilities of the system:
Turn on the transilluminator and place the Fluorescent Focus Target on the transilluminator surface.
NOTE: The BioDoc-It darkroom has a UV safety switch that turns the transilluminator off when the door
is open. After closing the door, be sure the overhead white light switch is turned off.
While watching the LCD screen, rotate the lens f-stop adjustment so that the image is bright enough to
see on the screen.
Rotate the lens focus adjustment on the lens. Adjust this so that the image appears in clear focus on the
LCD monitor. Note: Once the proper zoom range is set, the lens will have to be refocused when
zoomed in completely.
Rotate the zoom lens adjustment on the lens, so that the image is as big as possible. Readjust the
focus ring on the lens, making the image clear. Adjust the zoom so that the object of interest is within
the picture on the LCD monitor.
BioDoc-It and VisiDoc-It Imaging Systems 16
Service Procedures
Return Procedure
A Returned Goods Authorization (RGA) number must be obtained from UVP Customer Service before
returning any product.
Replacement Parts and Accessories
To order accessories or replacement parts for the BioDoc-It and VisiDoc-It System, contact UVP’s offices.
Part Description Part Number
Filter, Ethidium Bromide, 50mm Square 38-0220-01
Filter, SYBR Green, 50mm Square 38-0219-01
Filter, SYBR Gold, 50mm Square 38-0221-01
White Light Converter Plate, 21x26cm 38-0191-01
White Light Converter Plate, 25x26cm 38-0191-04
Visi-Blue Converter Plate, 21x26cm 38-0200-01
Visi-Blue Converter Plate, 25x26cm 38-0200-04
Gel-Cutter 85-0002-01
Gel-Ruler 85-0003-01
Gel-Scooper 85-0006-01
Gel-Tray, small 85-0007-01
Gel-Sentry DNA Preparation Plate 97-0076-01
Fluorescent Standard Step Tablet 33-0014-02
Doc-It®LS Analysis Software 97-0185-02
Spectacles, UV Blocking (UVC-303) 98-0002-01
Goggles, UV Blocking (UVC-503) 98-0002-02
Faceshield, UV Blocking (UVC-803) 98-0002-04
TroubleShooting
No Power to the Darkroom
Recheck main power cord connections to the darkroom.
Check fuses, located at the back of the unit, near the power port. You will need a flat-head screwdriver. Turn
the cap and the fuse holder will “pop-out”. Inspect the thin wire within the glass fus e to s ee if ther e is a brea k
in the wire. If so, replace fuse(s). If fuses are blown repeatedly, contact UVP Technical Support Department
for additional troubleshooting.
No Image on the LCD Screen
To power up the system, push once on the button located on the left side of the unit.
Touch Screen not Accurate
If touching or clicking on the buttons becomes inaccurate, you may need to recalibrate your touch screen.
Please follow all instructions in the touch screen calibration instructions included in your TS software CD.
BioDoc-It and VisiDoc-It Imaging Systems 17
Transilluminator Will Not Turn On
Be sure the darkroom cabinet’s door is completely closed. There is a UV exposure safety cut-off switch
that turns the transilluminator off when the darkroom cabinet’s door is opened.
Be sure the darkroom cabinet’s main power switch is lit. If not, refer to “No power to darkroom cabinet.”
The transilluminator itself has a power switch; make sure that the transilluminator switch is in the On
position.
Be sure the transilluminator’s power jumper cord is securely connected at both the ends of the cable
There is a 10-minute timer that turns off the transilluminator automatically after 10-minutes of use. The timer
switch located at the front of the darkroom can be reset (which restarts the 10-minute count).
Error Messages Pop Up on the Screen
Messages appear on the screen that might be related to the TS software interface, or Microsoft Windows. If
the message is related to Microsoft Windows, such as a reminder to activate or update your copy of
Windows, please contact your system administrator for assistance.
If an error message appears repetitively, and your system administrator does not recognize it as a Microsoft
Windows error, contact UVP Technical Support for further assistance.
Technical Support
UVP offers technical support on all of its products. If you have any questions about the product’s use,
operation or repair, please contact our offices at the locations below. Or go to UVP’s web site and click the
Tech Support > BioImaging Systems.
If you are in North America, South
America, East Asia or Australia:
If you are in Europe, Africa, the
Middle East of Western Asia:
Call (800) 452-6788 or (909) 946-
3197, and ask for Customer Service
during regular business days, between
7:00 am and 5:00 pm, PST.
E-mail your message to:
techsupport@uvp.com
Fax Customer Service, and send it to
(909) 946-3597
Write to: UVP, LLC 2066 W. 11th
Street, Upland, CA 91786 USA
Call +44(0) 1223-42002, and ask for
Customer Service during regular
business days between 9:00 am and
5:30 pm.
E-mail your message to: uvp@uvp.co.uk
Fax Customer Service, and send it to:
+44(0) 1223-420561
Write to: Ultra-Violet Products Ltd
Unit 1, Trinity Hall Farm Estate, Nuffield
Road, Cambridge CB4 1TG UK
BioDoc-It and VisiDoc-It Imaging Systems 18
Warranty
UVP's products are guaranteed to be free of defects in materials, workmanship and manufacture for one (1)
year from date of purchase. Consumable and disposable parts including, but not limited to tubes and filters, are
guaranteed to be free from defects in manufacture and materials for ninety (90) days from date of purchase.
Transilluminators carry a two (2) year warranty from da te of pu rcha se. If equip ment fail ure or mal function
occurs during the warranty period, UVP shall examine the inoperative equipment and have the op tion of
repairing or replacing any part(s) which, in the judg men t of U VP, were originally defective o r became so under
conditions of normal usage and service.
No warranty shall apply to this instrumen t, or part the reo f, that ha s been sub jec t to ac ciden t, negli gence ,
alteration, abuse or misuse by the end-user. Moreover, UVP makes no warranties whatsoever with respect to
parts not supplied by UVP or that have been installed, used and/o r serviced o ther than in strict compliance with
instructions appearing in this manual.
In no event shall UVP be responsible to the end-user for any incidental or consequential damages, whether
foreseeable or not, including but not limited to property damage, inability to use equipment, lost business,
lost profits, or inconvenience arising out of or connected with the use of instruments produced by UVP. Nor
is UVP liable or responsible for any personal injuries occurring as a result of the use, installation and/or
servicing of equipment.
This warranty does not supe rsed e any statutory rights that may be avail able in certain coun tries.
VisiDoc-It and BioDoc-It are trademarks of UVP, LLC. Doc-It is a registered trademark of UVP, L LC .
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