Trinity Amelung CS-190, Amax CS-190 Service manual
Service Manual
Instrument manufactured by
Heinrich Amelung GmbH
Lemgo, Germany
Juni, 98
Distributed by
Sigma Diagnostics
St. Louis, Missouri, USA
Table of Contents
1
1. Table of Contents
2. Introduction
2.1 Brief Description of the Operational
Characteristics of the AMAX CS-190
2.2 Traffic Lights
2.3 Pictograms
3. Safety Regulation
3.1 Information on How to Avoid Danger to Life and
Health
3.1.1 General Information
3.1.2 Information on the Repair of the AMAX CS-190
4. Accessories / Equipment
4.1 Measurement and Adjustment Tools for Repairs
5. Technical Data
5.1 Measurements
5.2 Weights
5.3 Electrical Connection Data
5.4 Temperatures
5.4.1 Temperatures of the Individual Modules
5.5 Intended Use
5.6 Throughput of the AMAX CS-190
1 – 1
2 – 0
2 – 1
2 – 2
2 – 2
3 – 0
3 – 1
3 – 1
3 – 1
4 – 0
4 – 1
5 – 0
5 – 1
5 – 2
5 – 2
5 – 3
5 – 3
5 – 4
5 – 5
6. Measuring Methods
6.1 Mechanical Measuring Mode
6.2 Optical Measuring Mode (Clotting)
6.3 Optical Measuring Mode (Chromogenic)
7. Instrument Installation
7.1 Instrument Overview
7.2 Installation of the AMAX CS-190
7.3 PC & Accessories
7.3.1 Jumper Settings of the AML-BUS-PC-Board
7.3.2 Jumper Settings of the Modem Boards
7.4 Printer Installation
7.5 Connections
7.5.1 Fresh Water / Waste Water System
7.5.2 Electrical Connections
7.6 Turning on the Instrument
6 – 0
6 – 1
6 – 2
6 – 4
7 – 0
7 – 1
7 – 2
7 – 3
7 – 4
7 – 4
7 – 5
7 – 7
7 – 7
7 – 8
7 – 9
June, 98 1 – 1
Table of Contents
8. Subassemlies
1
8 – 0
8.1 Dilutor
8.1.1 Adjusting the Temperature of the Pre-heater
8.2 Mechanical Measuring Unit
8.2.1 Removing the Measuring Unit
8.2.2 Adjusting the Measuring Block Temperature
8.2.3 Adjusting the PC Temperature Display
8.2.4 Adjusting the Inductive Ball Sensors
8.2.5 Adjusting the Hinge Mechanism Voltage (knee joint)
8.2.6 Adjusting the Rotational Speed
8.2.7 Adjusting the Motor Torque Voltage of the
Measuring Units
8.2.8 Adjusting the Dip-switches on the PH-M-MB
(D05601) Board
8.3 Cuvette Magazine
8.3.1 Removing the Cuvette Magazine or the Incubation
Rail
8.3.2 Adjusting the Mixer in the Incubation Rail (Q10005)
8.3.3 Adjusting the Ejector (Q10030)
8.3.4 Checking the Lifting Solenoid of the Incubation Rail
8.3.5 Adjusting the Temperature of the Incubation Rail
8.3.6 Adjusting the PC Temperature Display
8.3.7 Checking the Lifting Solenoids
8.3.8 Alignment Tools Magazine
8.3.9 Adjusting the Pusher 1 Unit
8.3.10 Alignment of the Cuvette Magazine in the
AMAX CS-190
8.3.11 Adjusting the Light Barriers and the Cuvette Comb
8.3.12 Adjusting the Cuvette Comb
8.3.13 Checking the Reflex Light Barriers
8.3.14 Checking the Conveyors
8.4 Reagent-Plasma-Barcode
8.4.1 Adjusting the Cooling Circuits
8.4.2 Adjusting the PC Temperature Display
8.4.3 Adjusting the Cutoff Voltage of the Servo Power
Amplifier
8.4.4 Adjusting the Mixer Voltage
8.4.5 Mechanical Reset Adjustments at the RPB
8.4.6 Adjusting the Barcode Scanner Position
8.5 Photometer
8.5.1 Removing the Measuring Unit
8.5.2 Adjusting the Temperature of each Measuring
Channel
8.5.3 Adjusting the Photometer Lamp Voltage
8.5.4 Adjusting the Photometer Measuring Amplifier
8.6 XYZZ-Robot (gantry)
8.6.1 Adjusting the Safety Systems of the Control Unit
June, 98 1 – 2
8 – 1
8 – 2
8 – 3
8 – 4
8 – 4
8 – 5
8 – 5
8 – 6
8 – 7
8 – 7
8 – 8
8 – 9
8 – 11
8 – 11
8 – 12
8 – 12
8 – 13
8 – 13
8 – 14
8 – 15
8 – 16
8 – 16
8 – 18
8 – 20
8 – 22
8 – 23
8 – 24
8 – 25
8 – 25
8 – 26
8 – 26
8 – 27
8 – 27
8 – 29
8 – 30
8 – 30
8 – 31
8 – 31
8 – 34
8 – 35
Table of Contents
1
8.6.2 Voltage Cutoff of the X-Motor Amplifier
8.6.3 Voltage Cutoff of the Y-Amplifier
8.6.4 Speed Cutoff of the X-Motor Amplifier
8.6.5 Mechanical Reset Adjustment of the XYZZ-Robot
8.6.6 Adjusting the XY-Motors
8.6.7 Adjusting the Level Sensors (liquid level sensors
and height sensors)
8.7 Water Temperatures
8.7.1 Adjusting the Water Reservoir II Temperature
(D10068)
8.7.2 Adjusting the PC Temperature Display
9. Maintenance
9.1 Refilling the System Fluids
9.2 Cleaning the Air Filter
9.3 Replacing the Photometer Lamp
9.3.1 Removing the Photometer Lamp
9.3.2 Inserting a New Lamp
9.4 Dilutor Syringe
9.4.1 Removing the Dilutor Syringe
9.4.2 Replacing the Teflon Seal
9.5 Cleaning the Photometer Channels
9.6 Pump Tubing, Waste Water Pump
9.7 Needle XYZZ-Robot
9.7.1 Replacing the Needle
9.7.2 Installing a New Needle
9.8 Cuvette Waste Drawer
8 – 35
8 – 36
8 – 37
8 – 38
8 – 39
8 – 40
8 – 42
8 – 43
8 – 43
9 – 0
9 – 1
9 – 2
9 – 3
9 – 3
9 – 4
9 – 5
9 – 5
9 – 6
9 – 7
9 – 8
9 – 9
9 – 9
9 – 10
9 – 11
10. Spare Parts List
Spare Parts List
11. Service Software
Description
11.1 Dilutor
11.2 Mechanical Measuring Unit
11.3 Cuvette Magazine
11.4 Reagent-Plasma-Tray
11.5 Photometer
11.6 XYZZ-Robot
11.7 Liquid Management
11.8 Text Viewer
11.9 Service Logbook
11.10 AMAX Teach in Program
11.10.1 Adjustable XY-Positions
11.10.2 Adjustable Z-Positions
11.11 Barcode Setup
11.12 AMAX Continuous Test Program
June, 98 1 – 3
10 – 0
10 – 1
11 – 0
11 – 1
11 – 6
11 – 11
11 – 15
11 – 19
11 – 23
11 – 33
11 – 38
11 – 41
11 – 42
11 – 43
11 – 47
11 – 49
11 – 51
11 – 52
Table of Contents
1
11.13 AMAX Continuous Test II
11.14 AMAX Self Test Program
11.15 Error Codes
11.16 AD Converter
11.17 Photometer Auto Align
11.18 Drip Test
12. Troubleshooting
12.1 Error Examples
13. Drawings
Dilutor Block Diagram 13 – 1
Dilutor Circuit Diagram 13 – 2
Dilutor 13 – 3
Mechanical Measuring Unit (Knee Joint) Block Diagram 13 – 4
Mechanical Measuring Unit Block Diagram 13 – 5
Measuring Block Heating Block Diagram 13 – 6
Mechanical Measuring Unit Circuit Diagram 13 – 7
Mechanical Measuring Unit 13 – 8
Cuvette Magazine PC Board PH-K1 Block Diagram 13 – 9
Cuvette Magazine PC Board PH-K5 Block Diagram 13 – 10
Cuvette Magazine Circuit Diagram 13 – 11
Cuvette Magazine 13 – 12
Slide Pusher I 13 – 13
Slide Pusher II 13 – 14
Incubation Rail 13 – 15
Reagent-Plasma-Tray Block Diagram 13 – 16
Reagent-Plasma-Tray Circuit Diagram 13 – 17
Reagent-Plasma-Tray 13 – 18
Photometer Block Diagram 13 – 19
Optical Measuring Unit Circuit Diagram 13 – 20
Optical Measuring Unit 13 – 21
Measuring Unit 13 – 22
XYZZ-Cantilever Arm Block Diagram 13 – 23
XYZZ Circuit Diagram 13 – 24
Cantilever Arm 13 – 25
Water Temperature Block Diagram 13 – 26
Water Circulation System Circuit Diagram 13 – 27
Water Temperature Control 13 – 28
Water Tank 13 – 29
11 – 53
11 – 54
11 – 55
11 – 55
11 – 56
11 – 57
12 – 0
12 – 1
13 – 0
June, 98 1 – 4
Introduction
2. Introduction
2
2.1 Brief Description of the Operational Characteristics
of the AMAX CS-190
2.2 Traffic Lights
2.3 Pictograms
2 – 1
2 – 2
2 – 2
June, 98 2 – 0
Table of Contents
Water Circulation System 13 – 30
Base Unit 13 – 31
Base Unit 13 – 32
Casing 13 – 33
Light Current Control 13 – 34
Photometer Amplifier 13 – 35
PC-AMAX Circuit Diagram 13 – 36
Wirering for AC/DC-Supply 13 – 37
Moduls PCB Location 13 – 38
1
14. LIS
14.1 Bidi-Protocol
14.1.1 Data Records
14.1.2 Header
14.1.3 Identification
14.1.4 Result /Test Order
14.1.5 ID-Query
14.1.6 Result
14.2 Communication Protocol
14.2.1 Master to Slave
14.2.2 Slave to Master
14.2.3 Command Characters and Symbols
14.2.4 Import
14.2.5 Result Transmission
14.2.6 Export
14.2.7 ID-Query
14.3 Host-Communication
14.3.1 Procedure of Processing Real-Time with Barcode
14.3.2 Master to Slave
14.3.3 Slave to Master
14.3.4 Result Transmission
14.3.5 Import of Requests (AMAX = MASTER)
14.3.6 Export of Results (AMAX = MASTER)
14 – 0
14 – 1
14 – 1
14 – 1
14 – 1
14 – 1
14 – 2
14 – 2
14 – 3
14 – 3
14 – 4
14 – 5
14 – 6
14 – 8
14 – 9
14 – 11
14 – 13
14 – 14
14 – 15
14 – 15
14 – 16
14 – 17
14 – 18
15. DOS Parameters
15.1 AMAX CS-190 DOS Parameters
15.2 Files in AMAX Directory
June, 98 1 – 5
15 – 0
15 – 1
15 – 3
Introduction
2.1 Brief Description of the Operational Characteristics of the
AMAX CS-190
• The AMAX CS-190 is a fully automated coagulation instrument.
• The AMAX CS-190 is used for measuring the in-
vitro coagulation times. Both plasma and whole
blood samples can be used.
• The instrument features an optical and a
mechanical measuring unit.
• In addition to the clotting tests, chromogenic
(kinetic) tests can be carried out by using the
optical measuring unit.
• The measured results can be converted to concentration or activity data with the
help of storable standard curves.
5.5
6.
5.5
2
• The PC identifies and stores patient samples and data.
• The samples are aspirated from their primary
containers with a needle by way of the XYZZrobot probe. Then the samples are mixed in a
measuring cuvette with either a reagent and/or a
buffer. After that the samples are incubated in the
incubation rail (12 incubation slots).
• At the end of the incubation period, the XYZZrobot moves the cuvette into the optical or
mechanical measuring unit.
8.6
8.3
8.6
6.1
6.2
6.3
• After the addition of the starting reagent by the XYZZ-robot, the coagulation time is
measured.
• Reagents, volumina and incubation periods can be programmed individually.
• Measured results and their calculations can be printed out in a protocol and stored
simultaneously in the PC’s patient archive.
• In addition, the results can be transferred to an external EDP (laboratory EDP) via
bidirectonial data transfer.
June, 98 2 – 1
2
Introduction
2.2 Traffic Lights
The traffic lights are positioned in the upper, right corner of the AMAX CS-190 next to
the dilutor unit (user position in front of the instrument). The light signals have the
following meanings:
Tab. 2.2.1
Light signal Meaning
no light
green light
yellow light WARNING: missing cuvettes, waste water reservoir full, fresh
red light ERROR: temperature outside operating range, photometer lamp
green + red light ERROR: XYZZ - waste water well full
running light CANCEL: press any key - Instrument is aborting current process.
AMAX CS-190 software booted, no errors registered, processing
not yet started.
AMAX CS-190 software booted, no errors registered, processing
started
water reservoir empty, plasma-reagent-tray cover open,
photometer lamp switched off
not yet ready
2.3 Pictograms
This service manual describes the maintenance and repair of the automatic coagulation
instrument AMAX CS-190.
These instructions are written for qualified medical and technical personnel.
IMPORTANT!
Read these instructions carefully and work only in the prescribed
manner!
Pay attention to warnings and notices!
Only in this way can damage to man and machine be avoided!
2 – 2 June, 98
Introduction
To increase the overall understanding of this manual, the following pictograms are
used:
ATTENTION!
There is DANGER TO LIFE of the user!
Read these instructions carefully and work only in the prescribed
manner!
Pay attention to warnings and notices!
Only in this way can damage to man and machine be avoided!
2
ATTENTION!
The AMAX CS-190 can be damaged!
Read these instructions carefully and work only in the prescribed
manner!
Pay attention to warnings and notices!
Only in this way can damage to man and machine be avoided!
IMPORTANT / NOTICE!
Requires additional operator action
or alteration of the adjustment parameters.
also see chapter
#
one turn to the ‘right’ (clockwise)
one turn to the ‘left’ (anti clockwise)
Amelung specific part number
1¬
1«
(Q00000)
Key on the PC-keyboard
June, 98 2 – 3
<X>
Safety Regulations
3. Safety Regulations
3
3.1 Information on How to Avoid Danger to Life and
Health
3.1.1 General Information
3.1.2 Information on the Repair of the AMAX CS-190
3 – 1
3 – 1
3 – 1
June, 98 3 – 0
Safety Regulations
3
It is guaranteed that the user is protected from danger to life and health
when using the AMAX CS-190 in compliance with the regulations (§ 3(1)
MedGV).
3.1 Information on How to Avoid Danger to Life and Health
3.1.1 General Information
• If the AMAX CS-190 shows any defects which might endanger patients or user
personnel, it must not be operated (§ 6(1) MedGV).
• Wear protective clothing, especially protective gloves. It is a possibility that infected
plasma (dangerous substances) might be touched.
• Protective clothing, especially protective gloves which were in contact with
dangerous substances (e.g. infected plasma) have to be changed and disposed
immediately (Technical rules for dangerous Substances (TRGS)).
• Plasma samples, reagents and waste water are toxic waste. The toxic waste has to
be disposed in compliance with the operative regulations (laboratory regulations).
3.1.2 Information on the Repair of the AMAX CS-190
• While under repair the instrument must not be used for testing.
• After a repair, the AMAX CS-190 has to undergo a functionality check. This involves
the carrying out of checking measurements. These actual values have to be
equivalent to the theoretical values.
ATTENTION!
Live parts (power unit and connection panel) must not be
touched!
DANGER TO LIFE!
ATTENTION!
On certain parts (photometer lamp or lamp casing) there is a
DANGER OF BURNS!
IMPORTANT!
Regular maintenance helps to avoid malfunctions and protects
the AMAX CS-190 from damage.
June, 98 3 – 1
Accessories / Equipment
4. Accessories / Equipment
4.1 Measurement and Adjustment Tools for Repairs 4 – 1
4
June, 98 4 – 0
Accessories / Equipment
4
4.1 Measurement and Adjustment Tools for Repairs
It is recommended to always carry the following tools and adjustment gauges at every
AMAX CS-190 service intervention:
Tab. 4.1.1
Measuring instruments / Tools Catalog no.
Thermometer 121563
Surface probe 121565
Immersion probe 121564
Digital multimeter
Oscilloscope incl. probes: 1:1 / 1:10
Adjustment gauge I (magazine light barriers) J01847
Adjustment gauge II (row pusher end light barrier) J01827
Adjustment gauge III (adjustment gauge lifting solenoid) J01819
Adjustment gauge (alignment pusher 1 unit) J01820
Needle ejector tool J01818
Spring balance 890300
Adjustment tool auto photometer height J01817
June, 98 4 – 1
Technical Data
5. Technical Data
5
5.1 Measurements
5.2 Weights
5.3 Electrical Connection Data
5.4 Temperatures
5.4.1 Temperatures of the Individual Modules
5.5 Intended Use
5.6 Throughput of the AMAX CS-190
5 – 1
5 – 2
5 – 2
5 – 3
5 – 3
5 – 4
5 – 5
June, 98 5 – 0
Technical Data
5.1 Measurements
5
AMAX CS-190
• Height
• Width
• Depth
Commander PC
• Height
• Width
• Depth
Monitor (14 inches)
• Height
• Width
• Depth
optional: Monitor (15 inches)
ca. 56 cm ( 22.5 inches)
ca. 82 cm (32.75 inches)
ca. 69 cm (27.75 inches)
ca. 12 cm ( 4.75 inches)
ca. 40 cm ( 16 inches)
ca. 44 cm (17.75 inches)
ca. 37 cm ( 15 inches)
ca. 35 cm ( 14 inches)
ca. 39 cm (15.75 inches)
• Height
• Width
• Depth
Keyboard
• Height
• Width
• Depth
Printer
• Height
• Width
• Depth
Base cabinet for the AMAX CS-190
• Height
• Width
• Depth
ca. 39 cm (15.75 inches)
ca. 39 cm (15.75 inches)
ca. 36 cm ( 14.5 inches)
ca. 4 cm ( 1.5 inches)
ca. 46 cm ( 18.5 inches)
ca. 17 cm ( 6.75 inches)
ca. 17 cm ( 6.75 inches)
ca. 36 cm ( 14.5 inches)
ca. 21 cm ( 8.5 inches)
ca. 71 cm (28.25 inches)
ca. 82 cm (32.75 inches)
ca. 69 cm (27.75 inches)
June, 98 5 – 1
5
5.2 Weights
Technical Data
• AMAX CS-190
• Base cabinet for AMAX CS-190
• Commander PC
• Monitor
• Printer
ca. 130 kg ( 286 pounds)
ca. 78 kg ( 172 pounds)
ca. 10 kg ( 22 pounds)
ca. 11 kg (24.2 pounds)
ca. 2,5 kg ( 5.5 pounds)
5.3 Electrical Connection Data
AMAX CS-190
• supply voltage
• supply frequency
• power consumption cold, max:
warm, min:
• heat emission
Commander PC
90-132 /
180-265
47-63 Hz
1240 kJ/h
VAC
690
230 VAVA
• supply voltage
• supply frequency
• power consumption
Monitor
• supply voltage
• supply frequency
• power consumption
Printer
• supply voltage
• supply frequency
• power consumption
90-132 /
180-264
50-60 Hz
145 VA
100-240 VAC
47-63 Hz
80 VA
230 VAC
50 Hz
30 VA
VAC
5 – 2 June, 98
Technical Data
5
5.4 Temperatures
The internal components of the Amelung Amax CS-190 are heated or cooled
automatically. In order to guarantee the perfect performance of the AMAX CS-190, the
following instructions have to be followed:
• The AMAX CS-190 must not be placed directly in front of a wall.
(minimum distance 15 cm)
• The ventilation grids must never be obstructed in any way.
(Cooling the AMAX CS-190 would be impossible)
• The system fluid supply has to be sufficient at all
times.
• To provide a sufficient air flow, the air filter has to
be clean.
• Do not expose the AMAX CS-190 to direct sunshine. The room temperature must
not exceed 32°C.
9.1
9.2
ATTENTION!
Disregard may lead to malfunction of and cause damage to the
AMAX CS-190!
5.4.1 Temperatures of the Individual Modules
• reagent plasma barcode tray ca. 16.5 ± 1.5°C
• incubation rail ca. 37.5 ± 0.3°C
• optical measuring unit ca. 37.5 ± 0.3°C
• mechanical measuring unit ca. 37.5 ± 0.3°C
• cuvette magazine ca. 37.5 ± 0.3°C
• pre-heater ca. 40.0 ± 0.5°C
• cooling water system ca. 25 - 55°C
• warm water circuit (37°C)
washing in the well ca. 38.5 ± 0.5°C
pumping in the well ca. 38.5 ± 0.5°C
The temperature in the well is measured by a liquid thermometer.
In the user software
is measured during the process “Wash” and “Fill”.
The warning limits for the temperatures can be entered in the user interface software.
(main menu), select “Maintenance”, then “Wash”. The temperature
In the case of a divergence, a message will be
displayed on the monitor and the status lights will
June, 98 5 – 3
2.2
5
show yellow.
In addition, an acoustic signal will sound and the AMAX CS-190 will not reactivate.
Technical Data
5.5 Intended Use
The AMAX CS-190 measures in-vitro coagulation times and chromogenic test
reactions. Both citrated whole blood and citrated plasma samples can be used.
You can choose between the mechanical measuring and the optical measuring mode.
Depending on the used reagents and measuring methods the following hemostasis
parameters can be determined:
• Prothrombin time (PT/Quick)
• Hepato-Quick
• Activated Partial Thromboplastin Time (APTT)
• Thrombin time (TT)
• Fibrinogen (Clauss)
• Fibrinogen (derived, optical density measurement)
• Normotest
• Thrombotest
• Factor assays: II, V, VII, VIII, IX, X, XI and XII
• AT III
• Plasminogen
• Antiplasmin
• PAI
• Heparin
• Protein S
• Protein C
• APC-Resistance
• and more
5 – 4 June, 98
Technical Data
5
5.6 Throughput of the AMAX CS-190
The throughput of the AMAX CS-190 is influenced by the type of measuring method
used.
Tab. 5.6.1
patients/hour patients/hour
PT-mechanical
(pipetting mode 0)
PT- mechanical
(pipetting mode 2)
PT-optical
(pipetting mode 0)
PT-optical, derived fibrinogen 240 120 60
APTT-mechanical or optical 110 110 55
Fibrinogen (Clauss) 115 115 57
AT III 80 80 40
Factor assays 120 120 60
PT-mechanical/APTT 120 60 30
PT-mechanical/APTT/AT III 90 30 15
tests/hour single double
180 180 90
240 240 120
190 190 95
June, 98 5 – 5
Measuring Methods
6. Measuring Methods
6
6.1 Mechanical Measuring Mode
6.2 Optical Measuring Mode (Clotting)
6.3 Optical Measuring Mode (Chromogenic)
6 – 1
6 – 2
6 – 4
June, 98 6 – 0
Measuring Methods
The AMAX CS-190 features a mechanical and an optical measuring unit.
The mechanical measuring unit has 4 measuring cells which work with the ball method.
The optical measuring unit (photometer) has 4 measuring channels for measurement of
the optical density. The wavelength of the light is 405nm.
The necessary total volumes for the measurements are:
• Mechanical measuring mode,
• Optical measuring mode
• Maximum volume (mechanical, optical): 600µl
The individual measuring modes are described on the following pages.
minimum volume: 75µl
, minimum volume: 150µl
6
6.1 Mechanical Measuring Mode
The time between the addition of the starting reagent and the beginning of the
formation of fibrin is measured.
The sample and if necessary the reagent and/or buffer are pipetted into a measuring
cuvette. After the incubation time it is placed into one of the mechanical measuring
channels automatically.
There is a special steel ball on the bottom of the cuvette.
With the addition of the starting reagent, the cuvette starts to rotate around its
longitudinal axes while the ball is held in a certain position by a magnet.
As coagulation begins, the ball is removed from its position by the forming fibrin
threads, because the force of the magnet is overcome. A sensor detects the change of
the ball’s position and ends time measurement.
Pic. 6.1.1
schematic display of the
mechanical measuring mode
cuvette
sensor
magnet
June, 98 6 – 1
6
A
Measuring Methods
Impulse measurement
In order to detect very unstable clots with the mechanical method, the continuous
rotation of the cuvette can be changed to a rotation with intervals after a predefined
time.
In the example on Pic 6.1.2, the continuous movement is switched to interval
movement after 25.6s. During the intervals, which prolong automatically, a clot cannot
be detected, but it has time to stabilise itself. The CV rises in dependence with the
length of the interval.
Pic. 6.1.2
Impulse measurement
Cuvette
rotates
Cuvette
stops
dd ition s tarting
reagent
activation
4s
Begin of
impulsemovement
25 .6 s
Movement:
½ rotation
pause
0.2s
pause
+0.1s
pause
+0.2s
pause
+0.3s
pause
+0.4s
6 – 2 June, 98
[s]
t
Measuring Methods
6
6.2 Optical Measuring Mode (Clotting)
The time between the addition of the starting reagent and the beginning of the
formation of fibrin is measured.
The sample and if necessary the reagent and/or buffer are pipetted into a measuring
cuvette. After the incubation time it is placed into one of the optical measuring channels
automatically.
The timing begins with the addition of the starting reagent. The intensity of the light
beam that passes through the cuvette is monitored by a sensor. When clots are
forming, the sample/reagent mixture becomes cloudy and the intensity of the light
reaching the sensor decreases (turbidimetrical measurement).
As soon as the change in the optical density reaches a pre-set threshold, the time
measurement stops.
Pic. 6.2.1 schematic display of the optical measurement (clotting)
Optical wave guide filter lens aperture cuvette aperture lens aperture sensor
Pic. 6.2.2
Analysis of the optical measurement (clotting)
mE
The coagulation time t2 is the time taken
from addition of the starting reagent to the
point when the absorbance change has
30
15mE
20
0
E
1-E
achieved the predefined threshold value.
The absorbance change is measured from
the stable baseline value at E
0.
Example: 15mE (threshold value).
10
0
E
The derived fibrinogen is calculated from
10 20 30
lag-
1
phase
Coag. time
t
t
2
the difference between the maximum
absorbance E
1 and the baseline value E0.
June, 98 6 – 3
6
Measuring Methods
6.3 Optical Measuring Mode (Chromogenic)
The rate of absorbance change is measured at predetermined intervals.
Sample and reagent are pipetted into a measuring cuvette and placed into one of the
optical measuring channels. After the addition of the starting reagent, measurements
are taken in defined intervals.
A monochrome light beam (405nm) passes through the cuvette. The change in optical
density is measured by a sensor behind the cuvette at pre-defined intervals (kinetic
measurement).
The values of change in mE/t are further mathematically processed.
Pic. 6.3.1 schematic display of the optical measurement (chromogenic)
Light source filter lens aperture cuvette aperture lens aperture sensor
Pic. 6.3.2
Analysis of the optical measurement
(chromogenic)
E
The absorbance increase within a
25mE
50mE/min
25mE
defined interval (30/60/90) is
measured and converted into
E/min.
The rate of absorbance change
must be constant (linear).
60
90
t[s]
6 – 4 June, 98
Instrument Installation
7. Instrument Installation
7
7.1 Instrument Overview
7.2 Installation of the AMAX CS-190
7.3 PC & Accessories
7.3.1 Jumper Settings of the AML-BUS-PC-Board
7.3.2 Jumper Settings of the Modem Boards
7.4 Printer Installation
7.5 Connections
7.5.1 Fresh Water / Waste Water System
7.5.2 Electrical Connections
7.6 Turning on the Instrument
7 – 1
7 – 2
7 – 3
7 – 4
7 – 4
7 – 5
7 – 7
7 – 7
7 – 8
7 – 9
June, 98 7 – 0
Instrument Installation
5
7.1 Instrument Overview
Pic. 7.1.1
6 7 8 9 10 11
7
1234
June, 98 7 – 1
7
Tab. 7.1.1
Instrument Installation
Overview
1. Mains switch 7.5
2. Cuvette magazine (front feed chute) 8.3 / 11.3
3. Cuvette magazine (front disposal chute) 8.3 / 11.3
4. Drawer (Disposal) 9.8
5. Mechanical measuring unit 8.2 / 11.2
6. Reagent-plasma-barcode 8.4 / 11.4
7. Robot (XYZZ) 8.6 / 11.6
8. Incubation rail 8.3 / 11.3
9. Photometer 8.5 / 11.5
10. Dilutor 8.1 / 11.1
11. Traffic lights 2.2
[Chapter]
7.2 Installation of the AMAX CS-190
1. Remove the packaging material of the AMAX CS-190 and level the instrument on
the base cabinet.
Fasten the connecting bolts which connect the base cabinet and the instrument.
2. Do not turn on the instrument yet!
All movable parts were secured for transport.
(XYZZ-robot: all axes were secured)
Remove all shipping clamps.
(check driving belt XY-axes, tapes, paper boards, etc.)
Check all movable parts for free movement.
3. Check the system coolant level,
if necessary fill up with original system liquid.
(830040 (500ml bottle)).
4. Configure PC & Accessories.
5. Connect the fresh/waste water reservoir and the
liquid level sensors.
6. Establish all electrical connections between the
PC and the AMAX CS-190.
7. Now the AMAX CS-190 can be turned on.
7 – 2 June, 98
9.1
7.3
7.5
7.6
Instrument Installation
ATTENTION!
Remove all shipping clamps and securing devices before turning
on the instrument.
Disregard may lead to malfunction of and cause damage to the
AMAX CS-190!
Packaging material is recycable!
7.3 PC & Accessories
7
1. Remove all packaging material of the PC, monitor, printer, etc.
In case the AML-Bus-Board (Board PH-PC (D02801)) or the modem board (Z04005)
have not yet been installed, the PC has to be opened and the boards have to be
placed into free slots.
Check and correct the jumper settings (see below) before the installation. Disregard
may lead to malfunction or conflicts with other components.
2. Connect all components (except for the PC and the AMAX CS-190!) and establish
all electrical connections.
3. Install the software:
First, switch on the PC, screen and printer. The PC installs its operating system
automatically (selection: WINDOWS 3.1) After approx. 30 min. the installation is
complete and you can continue by yourself. Deinstall WINDOWS with only the DOS
prompt remaining on the hard drive.
Now the main software can be installed.
It consists of three or five 3,5” disks:
a. Disk with main menu AMAX CS-190
b. Disk for service software
c. Disk for AMAX CS-190 (instrument specific data)
d. Disk for modem software (option)
e. Disk for printer driver
(printer specific)
7.4
June, 98 7 – 3
7
7.3.1 Jumper Settings on the AML-BUS-PC-Board
There are two dip-switches on the Board PH-PC for adjusting the address.
The following switch adjustments have to be made so that the board can be identified:
Tab. 7.3.1
Dip-switch S1 Position Dip-switch S2 Position
Switch 1 on Switch 1 on
Switch 2 on Switch 2 off
Switch 3 on Switch 3 off
Switch 4 off Switch 4 off
ATTENTION!
Disregard may lead to malfunction of the AMAX CS-190!
Instrument Installation
7.3.2 Jumper Settings of the Modem Boards
The different modems are not distinguishable by their names. For their differentiation,
the BZT-No. is used here. This BZT-No. is printed on the packaging and on a label on
the modem itself.
Tab. 7.3.2
Designation Switch position for Com 3 IRQ 5
Internal fax-modem BZT-No.
(Company)
BZT - No. A109 822D
BZT - No. A107 434D
(YAKUMO)
BZT - No . A121 250F
(CREATIX)
BZT - No. A106 901D
The initialisation string for these modems is:
ATE0Q0V0L1X3%C1\N3\V1S0=2S7=100^M
2 and 7 (7 is a soldering bridge)
switch 1 and 6 to ON
all others to Off
switch 1 and 6 to ON
all others to Off
2,3,8
ATTENTION!
Disregard may lead to malfunction of the AMAX CS-190!
7 – 4 June, 98
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