TRENDnet 18-1106-33 User Manual
MultiTemp®III
User Manual
18-1106-33
Edition AD
A
P H A R M A C I A B I O T E C H
Reading this entire manual
is necessary for full understanding and safe use of
this product.
Should you have any comments on
this manual, we will be pleased to
receive them at:
Pharmacia Biotech AB
S-75182 Uppsala
Sweden
Pharmacia Biotech reserves the right to
make changes in the specifications
without prior notice.
Warranty and Liability
Pharmacia Biotech AB guarantees that
the product delivered has been
thoroughly tested to ensure that it
meets its published specifications. The
warranty included in the conditions of
delivery is valid only if the product has
been installed and used according to
the instructions supplied by Pharmacia
Biotech AB.
Pharmacia Biotech AB shall in no
event be liable for incidental or consequential damages, including without
limitation, lost profits, loss of income,
loss of business opportunities, loss of
use and other related exposures, however caused, arising from the faulty
and incorrect use of the product.
Copyright©1997 Pharmacia
Biotech AB
All rights reserved. No part of this
publication may be reproduced, stored
in a retrieval system or transmitted in
any form by any means, without permission in written form from the
company.
Important user information
Contents
1. Introduction ..................................................................... 3
2. Safety information ........................................................... 3
2.1 Safety precautions................................................. 3
2.2 Safety standards.................................................... 4
2.3 Safety features ...................................................... 5
3. Installation ....................................................................... 6
3.1 Unpacking and installation................................... 6
3.2 Tubing connections............................................... 7
3.3 Recommended bath liquids................................... 7
3.4 Filling and draining the bath ................................ 8
3.5 Mains connection................................................. 8
4. Operation ........................................................................ 9
4.1 Start-up.................................................................. 9
4.2 Setting the temperature limit .............................. 10
4.3 Temperature setting limit .................................... 10
4.4 Temperature setting............................................ 11
4.5 Temperature correction value............................. 11
5. Trouble shooting..............................................................13
5.1 Error messages ......................................................13
6. Maintenance ....................................................................14
6.1 General.................................................................14
6.2 Fuses.....................................................................14
7. Detailed Description ........................................................16
7.1 General operating principle...................................16
7.2 Front and rear panels............................................16
7.3 Control panel........................................................16
7.4 Description of safety features................................18
7.5 Manual restart in operation mode 8.....................20
8. Technical specification .....................................................22
9. Ordering information.......................................................23
Short instructions (Back page)
2
1. Introduction and 2. Safety information
1. Introduction
MultiTemp®III circulates constant temperature liquid to
external electrophoresis equipment. The liquid temperature
range is -10 to +90 °C. The unit contains KLEA 134a*, an
ozone friendly HFC-type coolant.
MultiTemp III both supplies coolant for high voltage techniques and provides temperature control at higher temperature
(e.g. 60 °C) when performing DNA sequencing. When used
with equipment such as the Pharmacia Biotech Multiphor
®
II
electrophoresis unit, two units can be connected to
MultiTemp III at the same time.
MultiTemp III can also be used as a stand-alone constant
temperature water bath.
*KLEA 134a is the Trademark of ICI.
2. Safety information
Read this entire manual before using MultiTemp III.
1. This instrument is designed for indoor use only.
2. The unit must be connected to a grounded mains socket.
3. Ensure that the vents at the sides of the instrument are not
covered.
4. Do not use in a dusty atmosphere or close to spraying
water.
5. Set the temperature limit cut-off correctly.
6. Use only water or water with ethylene glycol in the bath.
3
2.1 Safety
precau-
tions
1. Introduction and 2. Safety information
7. Do not use the circulator beyond its specifications or for
other than its intended use.
8. Do not touch the stainless steel bath cover and tubings
when the unit is used at a high working temperature. Under
these conditions, the surface temperature can rise up to
approximately 70 °C.
MultiTemp III is designed in accordance with:
• Protection class 1 according to IEC 536: all conductive
parts that might become accessible are connected to earth.
• Protection IP 32 according to IEC 529/EN 60529: protection against accidentally touching accessible live parts by
penetration of particles with a thickness or a diameter of
more than 2.5 mm and protection against dropping water
onto the unit, inclined at max. 15 degree angle.
This product meets the following EG directives:
• 72/23/EEC through the harmonised standard;
EN 61010-1, Safety requirements for electrical equipment
for measurement, control and laboratory use.
- Part 1: General requirements.
- Part 2: Requirements for laboratory Equipment for heating of material.
Note: the operator has to apply the safety precautions listed in section 2.1 above.
• 89/336/EEC through the standards;
- EN 50081/Part 1:03.93 Electromagnetic Compatibility
emission standard
- EN 50082/Part 1:03.93 Electromagnetic Compatibility,
Generic immunity standard.
The CE symbol, and corresponding declaration of confirmity, is valid for the instrument when it is
- used as a “stand alone” unit or
- connected to other CE marked Pharmacia Biotech
instruments or
- connected to other products recommended or descri-
bed in this manual and
- is used in the same state as it was delivered from
Pharmacia Biotech AB except for alterations described in
this manual.
4
2.2 Safety
standards
1. Introduction and 2. Safety information
General cut-off
The heater, the compressor and the circulation pump can be
switched off by a relay built into the unit. This general cutoff will be activated if a malfunction is detected by the safety
sensors.
See 7.4, Description of Safety Features.
Protection against over-heating
A built-in temperature limit switch protects against uncontrolled heating of the bath liquid by activating the general
cut-off if this limit is exceeded.
The built-in temperature limit switch also senses low levels of
coolant.
See 7.4, Description of Safety Features.
Pump motor protection
When the temperature in the winding of the pump motor
reaches a certain level, the general cut-off will be activated,
preventing the motor from overheating.
See 7.4, Description of Safety Features.
Sensor check
The temperature sensor is permanently monitored. The general cut-off is activated if sensor breakage or current leakage is
detected.
See 7.4, Description of Safety Features.
Restart after power failure
MultiTemp III automatically restarts after a power failure.
See 7.4, Description of Safety Features.
5
2.3 Safety
features
3. Installation
3. Installation
1. Check the contents against the packing list supplied. Inspect
for any damage that may have occurred during transit. Report
any damage immediately to your local Pharmacia Biotech
representative and to the transport company concerned.
2. Place MultiTemp III in its working location, ensuring adequate space for ventilation of air through the grids on the
sides (air outlet) and rear (air inlet) of the unit.
3. Allow MultiTemp III to stand in an upright position for
24 hours before use. Compressor lubricant may have run
into the liquifier and evaporator if the unit has not been
transported in an upright position.
Fig. 1.
Front and rear view of MultiTemp III.
1. Control Panel
2. Temperature limit dial
3. Outlet nozzle
4. Inlet nozzle
5. Mains cable
6
6. Circuit breaker
220 V, two thermal fuses
115 V, one thermal fuses
7. Bath Drain
8. Handles (front and rear)
9. Cover lid
10. Air ventilation
1.
9.
6.
5.
8.
7.
8.
10.
2.
3.
4.
3.1 Unpacking
and
installation
3. Installation
Silicone tubing (inner diameter 8 mm) can be used over the
entire working temperature range of the unit. PVC tubing
can only be used in the temperature range of 10 to 60 °C.
1. Connect the inlet and outlet tubing to the nozzles on the
rear of the circulator (Fig. 1).
2. Connect the other ends to the electrophoresis unit. The
nozzles have an outer diameter of 9.5 mm. To ensure safe
tubing connections, secure all tubing with hose clamps.
When MultiTemp III is used as a water bath, the nozzles
should be connected to each other.
Condensation of water on cold tubing can be avoided by
covering it with insulating tubing. (See Insulation for cooling
tubing in the Ordering Information section.)
Condensation on the cooling plate of Multiphor II during
sample application can be avoided by shutting off the circulation to the cooling plate with a three way valve. (See 3-way
Valve Set in the Ordering Information section.)
Note: Do not touch or remove tubing when the unit is in
operation. Stay alert to the dangers of hot water. The
surface temperature of the tubing can reach 70 °C at
high working temperatures.
Use distilled water in the temperature range of 5 to 90 °C. A
mixture of distilled water and ethylene glycol in a concentration up to 20% is suitable for lower ranges (-10 to +40 °C).
Warning! Ethylene glycol is poisonous. Handle with care and
follow recommended safety procedures.
Warning! Methanol and ethanol are not recommended because of their low flash points. Both liquids are unsafe to use.
Remove the plastic lid and fill the bath with liquid. The distance between the surface of the liquid and the stainless steel
top plate should be about 2 cm. If it is more than 4 cm, a
sensor will activate the temperature limit switch and cause
cut-off.
To drain the bath, switch off the mains power and remove
the outlet tubing from the external equipment and let the
7
3.2. Tubing
connections
3.3 Recommended
bath
liquids
3.4 Filling and
draining
the bath
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