Thermo Scientific Genesys GENESYS 30, GENESYS 50, GENESYS 180, GENESYS 40, GENESYS 140 Instruction Manual
...
UV-Visible
GENESYS Family
Spectrophotometers
User Guide
269-331300 Revision A February 2018
© 2018 Thermo Fisher Scientific Inc. All rights reserved.
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The contents of this document are subject to change without notice. All technical information in this
document is for reference purposes only. System configurations and specifications in this document supersede
all previous information received by the purchaser.
Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate or errorfree and assumes no responsibility and will not be liable for any errors, omissions, damage or loss that might
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For Research Use Only. This instrument or accessory is not a medical device and is not intended to be used
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WARNING Avoid an explosion or fire hazard. This instrument or accessory is not
designed for use in an explosive atmosphere.
C
Contents
Chapter 1 GENESYS Spectrophotometers. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1
Considerations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Site Preparation and Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Operating Precautions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Spectrophotometer Basics - GENESYS 40 and GENESYS 50. . . . . . . . . . . . . . . 3
Sample Compartment - GENESYS 40 and GENESYS 50 . . . . . . . . . . . . . . . 3
Single Cell Holder - GENESYS 40 and GENESYS 50 . . . . . . . . . . . . . . . . . . 4
Optional Sample Holders - GENESYS 40 and 50. . . . . . . . . . . . . . . . . . . . . . 5
Cell Holder Replacement - GENESYS 40 and 50 . . . . . . . . . . . . . . . . . . . . . . 6
Spectrophotometer Basics - GENESYS 140, GENESYS 150, GENESYS 180
and BioMate 160. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Sample Compartment - GENESYS 140, GENESYS 150, GENESYS 180
& BioMate 160 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Single Cell Holder - GENESYS 140, GENESYS 150, GENESYS 180 &
BioMate 160 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Optional Single Sample Holders - GENESYS 140, GENESYS 150,
GENESYS 180 and BioMate 160 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Disposable 50 mL Microcell Holder – GENESYS 140, GENESYS 150,
GENESYS 180 and BioMate 160 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Beckman Cell Holder – GENESYS 140, GENESYS 150,
GENESYS 180 and BioMate 160 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Peltier Thermostatted Single Cell Holder – GENESYS 140,
GENESYS 150, GENESYS 180 and BioMate 160 . . . . . . . . . . . . . . . . . . 11
Sipper Accessory - GENESYS 140, GENESYS 150, GENESYS 180
and BioMate 160 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Fiber Optic Coupler and Probe (UV Vis systems only) . . . . . . . . . . . . . . . . . 21
Spectrophotometer Basics – All Models. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Halogen Lamp. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Lid Removal and Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Chapter 2 GENESYS On-board Software. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .33
GENESYS On-board Software Home Screen and Navigation. . . . . . . . . . . . 33
Getting Started . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Thermo Scientific GENEYS Family Spectrophotometers i
Contents
Viewing Saved Experiment Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Application Home. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Creating A New Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Experiment Execution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
Data Analysis and Actions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Live Display Application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
Fixed Application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Scan Application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Quant Application. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
Calibrated and Uncalibrated Quant Methods . . . . . . . . . . . . . . . . . . . . . . . . 47
Cell Changer Setup in Quant . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
C-Mode. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Kinetics Application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Importing Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
Exporting Data and Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
Performance Verification Tests and Reports . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
Running Performance Verification Tests . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
Customized Performance Verification Tests . . . . . . . . . . . . . . . . . . . . . . . . . 54
Customizing Stray Light Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
Customizing Wavelength Accuracy Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
Customizing Photometric Accuracy Test. . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
Accessory Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
Cell Changer Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
Sipper Configuration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
Measurements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
Calibrated Sipper. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
Calibrating the Sipper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
Custom Workflows . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
Peltier Configuration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
Accessory Configuration in Scan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
SmartStart . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
Configuring and Switching to SmartStart Mode . . . . . . . . . . . . . . . . . . . . . . 75
Exiting SmartStart Mode. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
Connecting to VISIONlite . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
Enable the VISIONlite Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
Printer Setup and Wi-Fi Printing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
Setting Up USB Printer. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
Setting Up Thermal Printer. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
Configuring Network Paths. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
ii GENEYS Family Spectrophotometers Thermo Scientific
Contents
Chapter 4 BioMate 160 Applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .87
OD600 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
dsDNA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
RNA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
Protein BCA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
Protein Bradford . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90
Protein Lowry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91
Protein Pierce 660 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92
Protein Rapid Gold BCA. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
Thermo Scientific GENEYS Family Spectrophotometers iii
Contents
iv GENEYS Family Spectrophotometers Thermo Scientific
1
GENESYS Spectrophotometers
This user guide applies to the following spectrophotometers:
• Intermediate capability models – the Thermo Scientific™ GENESYS™ 50 platform – with
sample compartments configured for single samples only:
– GENESYS™ 40 Vis
– GENESYS 50 UV-Vis
• Advanced capability models – the GENESYS™ 150 platform – with sample
compartments configured for:
–single samples
– multi-cell changer option
– Peltier thermostatted cell holder option
– fiber optic probe option
• GENESYS™ 140 Vis
• GENESYS 150 UV-Vis
• GENESYS™ 180 UV-Vis
•BioMate™ 160
Note There is also a GENESYS™ 30 Vis that shares the sample compartment
configuration of the GENESYS 50 platform but offers a user experience with a tactile
rubber keypad, high resolution non-touch color 5-inch screen and simplified software.
The GENESYS 30 has a separate dedicated user guide
Thermo Scientific GENEYS Family Spectrophotometers 1
1
GENESYS Spectrophotometers
Considerations
Considerations
This document uses the following conditions:
DANGER Indicates a hazardous situation which, if not avoided, will result in death or
serious injury.
WARNING Indicates a hazardous situation which, if not avoided, could result in death or
serious injury.
CAUTION Indicates a hazardous situation which, if not avoided, could result in minor or
moderate injury.
NOTICE Follow instructions with this label to avoid damaging the system hardware or
losing data.
Note Contains helpful supplementary information.
Site Preparation and Safety
Before using the system, read the site preparation and safety manual on the documentation
media provided. Always follow the safety precautions in that manual and in this document
when using the system.
Operating Precautions
WARNING Do not operate this system without following the safety precautions described
in this manual and the documentation that came with your system.
The spectrophotometer contains precise optical components. Handle it carefully and follow
these precautions.
• Do not allow moisture to leak into the instrument interior
• Wipe off spilled chemicals immediately
• Do not drop the instrument
• Protect the instrument from mechanical shock
• Protect the instrument from dust
2 GENEYS Family Spectrophotometers Thermo Scientific
1
Lift here
Closure magnet
Light beam path
Detector lens
Monochromator
window
Tray alignment
posts
Drain
GENESYS Spectrophotometers
Spectrophotometer Basics - GENESYS 40 and GENESYS 50
Spectrophotometer Basics - GENESYS 40 and GENESYS 50
Remove all tape from the exterior of the instrument and inside the sample compartment.
Sample Compartment - GENESYS 40 and GENESYS 50
Thermo Scientific GENEYS Family Spectrophotometers 3
High durability constant torque hinges hold
the lid at any angle
Window and lens protect interior optics from spills and
vapors.
Magnet at front holds lid closed to exclude
light when door is lowered
Sample holder tray aligns to posts on baseplate.
Excess spills drain to benchtop.
1
Locater pin
Magnets
GENESYS Spectrophotometers
Spectrophotometer Basics - GENESYS 40 and GENESYS 50
Single Cell Holder - GENESYS 40 and GENESYS 50
Standard 10 mm cuvette holder Underside of a single cell holder
Tray features
• Able to contain spills up to 150 mL
• Can be removed by pulling up on the cell holder
• Can be washed in the sink or a dishwasher - dry promptly!
NOTICE
• Clean the tray with water and mild detergent. Ethanol and iso-propyl alcohol can be
used if necessary but do not soak the tray in alcohols.
• Do not allow acetone, chlorocarbons or other aggressive organic solvents to contact
the tray. The PC-ABS plastic will soften and discolor.
Removal – grasp cell holder and lift up and forward
Insertion – allow front magnet to engage. Lower cell holder into place, allowing back
magnet to guide and engage
4 GENEYS Family Spectrophotometers Thermo Scientific
Optional Sample Holders - GENESYS 40 and 50
Cell holder trays equipped to position other kinds of cells and samples are available. They
insert and remove in the same way as the standard cell holder.
Te st tu b e h o ld e r
Tall test tube adapter
1
GENESYS Spectrophotometers
Spectrophotometer Basics - GENESYS 40 and GENESYS 50
Long path rectangular cell
holder
Long path cylindrical cell
holder
Filter holder
Accessory kit for hose
entry/exit
Thermo Scientific GENEYS Family Spectrophotometers 5
1
Lift here to open
Lid hold-down
magnet
GENESYS Spectrophotometers
Spectrophotometer Basics - GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160
Cell Holder Replacement - GENESYS 40 and 50
Thermostatted cell holder and Adjustable filter holder accessories are supplied without a tray.
Loosen the captive screw at the base of the cell holder to remove it. Attach the new sample
holder in the same way.
Note The Accessory Kit for Hose Entry/Exit is required to support use of the
thermostatted cell holder. See Lid Removal and Replacement for additional installation
instructions.
Spectrophotometer Basics - GENESYS 140, GENESYS 150,
GENESYS 180 and BioMate 160
Remove all tape from the exterior of the instrument and inside the sample compartment.
Sample Compartment - GENESYS 140, GENESYS 150, GENESYS 180 & BioMate 160
High durability constant torque hinges hold
the lid at any angle
Magnet at front holds lid closed to exclude
light when door is lowered
6 GENEYS Family Spectrophotometers Thermo Scientific
1
Alignment
posts
Opto-sensor
Drain
Detector lens
Monochromator
window
GENESYS Spectrophotometers
Spectrophotometer Basics - GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160
Align arrow on sample holder base with
arrow on sample compartment and groove
on sample compartment wall.
Window and lens protect interior optics
from spills and vapors.
Sample holder aligns to posts on baseplate
and groove.
Excess spills drain to benchtop. Opto-sensor
is used for positioning cell changer.
Thermo Scientific GENEYS Family Spectrophotometers 7
1
Locater pin
Ring magnet
GENESYS Spectrophotometers
Spectrophotometer Basics - GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160
Single Cell Holder - GENESYS 140, GENESYS 150, GENESYS 180 & BioMate 160
Standard 10 mm cuvette holder
NOTICE
• Clean cell holders by wiping with a solution of mild detergent. Ethanol and isopropyl
alcohol can be used if necessary but do not submerge the cell holder in liquid.
• Do not allow acetone, chlorocarbons or other aggressive organic solvents to contact
the cell holder or base. The PC-ABS plastic will soften and discolor.
Removal—grasp blue knob and lift straight upInsertion—align arrow on left side of sample
compartment with arrow on sample holder
accessory. Lower cell holder into place.
Magnet holds accessory down. Pin under
accessory base aligns cell holder in beam and
prevents rotation.
8 GENEYS Family Spectrophotometers Thermo Scientific
1
GENESYS Spectrophotometers
Spectrophotometer Basics - GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160
Optional Single Sample Holders - GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160
Cell holder trays equipped to position other kinds of cells and samples are available. They
insert and remove in the same way as the standard cell holder.
Long path rectangular cell holder
Long path cylindrical cell holder
Filter holder
Disposable 50 μL Microcell Holder
Beckman cell holder
Peltier Thermostatted cell holder
Note Water thermostatted cell holders are not supported on this platform. See the Peltier
Thermostatted Cell Holder accessory.
Thermo Scientific GENEYS Family Spectrophotometers 9
1
GENESYS Spectrophotometers
Spectrophotometer Basics - GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160
Disposable 50 mL Microcell Holder – GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160
Disposable plastic microcells with 50 μL chambers located at a Z-height of 8.5 mm and
centered on the cell are supported by this accessory. At time of writing of this user guide, the
Eppendorf™ UVette™ is the only brand supported, but see the insert with the accessory for
updates. Cuvettes made by BrandTech™ are not supported because of poor manufacturing
reproducibility in these cells.
Note Plastic microcells are not as transparent as quartz, especially at very short
wavelengths. The combination of absorption by the cell and masking of the
spectrophotometer beam required to restrict it to the 2.5 mm tall cell window reduces the
energy passing through these cells. Photometric performance will be lower with these cells
than with full size quartz cells.
Beckman Cell Holder – GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160
Beckman™ pioneered the use of a special type of microcell with volumes of 100 μL or less.
These very short cells require a special holder to accommodate them at the appropriate
Z-height for the cell and to mask off part of the spectrophotometer beam to ensure that light
only passes though the sample, and not over it.
10 GENEYS Family Spectrophotometers Thermo Scientific
1
GENESYS Spectrophotometers
Spectrophotometer Basics - GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160
Peltier Thermostatted Single Cell Holder – GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160
The accessory holds a single cell at a temperature between 20° and 60 °C with a precision of
≤0.2 °C and an absolute accuracy of ±0.5 °C.
Note The specification for absolute accuracy is limited by the accuracy of the
thermocouple used in construction of the device. ±0.3° C is the specification of a superior
quality thermocouple. Beyond this specification the cost of the thermocouple becomes
exponentially higher and use of a calibrated thermocouple becomes a necessity if
exceptionally high accuracy is a requirement.
In our tests, using a calibrated thermocouple probe, we generally find the temperature of the
solution in the cuvette to fall within ±0.3 °C of the programmed absolute temperature.
The temperature measured and reported by the device is the temperature of the metal
cell-holder block. The temperature of stirred liquid in a full size quartz cell will reach the same
temperature as the cell holder block after approximately 6 minutes when heating from 23 °C
to 35 °C. This is only a guide. Performance will vary if:
• The initial temperature is different
• The final temperature is below room temperature
• The cuvette is not stirred
• The cuvette is not made of glass or quartz
• The cuvette is a micro-cuvette
Thermo Scientific GENEYS Family Spectrophotometers 11
1
GENESYS Spectrophotometers
Spectrophotometer Basics - GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160
The experimental parameters for controlling the Peltier thermostatted cell holder include an
equilibration time. This is a “wait time” between when the cell holder block reaches the target
temperature and when the absorbance measurement is made. We strongly recommend that
you place the probe of a digital thermometer or thermocouple of sufficient accuracy and
precision in the liquid in a cell under what will be normal experimental conditions, and bring
a quantity of appropriate matrix (solvent or buffer) to temperature in the cell holder while
monitoring the temperature to determine performance under your experimental conditions.
A white paper describing important considerations and limitations in thermostatting
experiments is included with this documentation set. Refer to Introduction to Temperature
Control for additional details.
In brief, however:
• Quartz conducts heat six times faster than plastic – samples in quartz cells come to
temperature much faster than they do in plastic cells.
• A full size cell takes approximately 66% more time to reach equilibrium temperature if it
is not stirred.
Installing the Accessory
Align white arrows as shown.
• Liquid in unstirred plastic cells did not heat to within 0.6 °C of the cell holder block
temperature when heating in our experiments.
• Plastic microcells and semi-microcells have significantly reduced surface contact with the
walls of the cell holder block compared to full size plastic cells or glass/quartz reduced
volume cells. If using a reduced volume plastic cell it is essential that you perform some
experiments to determine the appropriate equilibration time setting.
12 GENEYS Family Spectrophotometers Thermo Scientific
1
GENESYS Spectrophotometers
Spectrophotometer Basics - GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160
Lower the cell holder into the sample compartment so that the V engages with the groove on
the side of the sample compartment and the hold-down magnet engages
Connect cables as shown.
• The power supply is identical to the instrument power supply
• Operate this accessory with the sample compartment lid open
• Do not close the sample compartment lid while the accessory is running
• Optional – the sample compartment lid can be removed while using this accessory
See “Peltier Configuration” on page 69 of this guide for instructions on configuring your
method to use this accessory.
Thermo Scientific GENEYS Family Spectrophotometers 13
1
GENESYS Spectrophotometers
Spectrophotometer Basics - GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160
Sipper Accessory - GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160
Introduction to Sipper Use
A detailed introduction to sippers and how they can be used to get the best results in different
situations is provided in a separate document (English language only) included in the
documentation media with this user guide.
Components of the Sipper System
Complete sipper systems come with the following components
Item Photograph
Sipper pump
Sample compartment insert with
gooseneck
14 GENEYS Family Spectrophotometers Thermo Scientific
Spectrophotometer Basics - GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160
3 m PTFE transfer tubing
1 Flow cell, 160 µL volume
with screw connector set
Smaller volume flow cells are available but the smaller window size cuts off some of the spectrophotometer beam, reducing photometric performance. If the absorbance values measured
are below 1.0, however, this will not make a noticeable impact on data quality.
Connector package
The connectors supplied are designed for use with the # 16 tubing supplied. They can also
be used with smaller # 14 tubing with extra effort. To use larger # 25 tubing please purchase
a larger connector separately.
Item Photograph
30 cm Silicone pump tubing
(#16 size – 1.6 mm wall,
1.6 mm inside diameter)
1
GENESYS Spectrophotometers
Thermo Scientific GENEYS Family Spectrophotometers 15
1
Inlet marker
Attach long
connector to inlet
GENESYS Spectrophotometers
Spectrophotometer Basics - GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160
Compatibility of the GENESYS Sipper with tubing sold by us for use with
spectrophotometer sippers.
Material / Size #13 #14 #16 #25
Silicone
C-Flex
Viton
ChemDurance Bio
a
Chemdurance Bio tubing is suitable for use with the Smart Sipper
for Evolution spectrophotometers
a
Connecting the Sipper Tubing
1. Connect screw connectors to flow cell.
16 GENEYS Family Spectrophotometers Thermo Scientific
1
GENESYS Spectrophotometers
Spectrophotometer Basics - GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160
2. Attach the gooseneck tube.
3. Lower the cell holder into the sample compartment so that the V engages with the groove on the side of the sample compartment and the hold-down magnet engages.
4. Position sipper pump and connect cables.
Note The power supply for the pump is identical to the power supply for the
spectrophotometer. There is no risk or danger to connecting “the wrong one”.
Thermo Scientific GENEYS Family Spectrophotometers 17
1
Pump head connection taking
silicone tubing to waste
GENESYS Spectrophotometers
Spectrophotometer Basics - GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160
5. Decide where the waste container will be placed.
• If very close to the pump, run the pump tubing directly to the waste container.
6. If farther, run (less expensive) PTFE transfer tubing from the pump-head tubing to the waste container.
7. Determine the length of pump tubing required for your configuration. Minimum recommended length of pump tubing is 10 cm.
8. Cut a suitable length of pump head tubing and lay it on the bench in front of the pump.
9. Place the flow cell in the cell holder with the windows in the beam (facing to the front and back).
18 GENEYS Family Spectrophotometers Thermo Scientific
1
Zip tie
Turn right to tighten
GENESYS Spectrophotometers
Spectrophotometer Basics - GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160
10. Route the PTFE cell connector tubing from the inlet (long connector) through the gooseneck.
Refer to separate Introduction to Sipper Use for detailed information.
11. Route the PTFE cell connector tubing from the outlet (short connector) to the pump.
12. Trim all cell connector tubing to an appropriate length for your use scenario.
13. Attach the pump-head tubing to the PTFE tubing using the couplers.
Secure the connections with zip-ties if using high pump speeds or tubing larger than the
size supplied.
14. Install the pump-head tubing into the pump head as shown.
15. Test the system for leaks.
Use the table on the pump to choose a pump speed suitable to the volume that you wish
to pump in your procedure.
Note Accuracy of the volume delivered decreases as pumping speed and tubing size
increase.
Thermo Scientific GENEYS Family Spectrophotometers 19
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GENESYS Spectrophotometers
Spectrophotometer Basics - GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160
Program a custom sequence to sip at the chosen speed for 60 seconds, or longer if
necessary. Place the inlet tube in a beaker of clean solvent and pump for long enough to
completely fill the system with solvent and deliver it to the waste container.
Repeat the sip sequence while observing the PTFE tubing between the flow cell and the
pump. There should be no bubbles in the tubing. If there are, check the tubing
connections to the flow cell.
Remove the flow cell from the cell holder and check it for solvent leaks.
Check the connections between the PTFE tubing and the pump-head tubing for solvent
leaks.
16. Follow the process to calibrate the sipper.
Failure to deliver a consistent volume can be an indicator of an air leak at the connection
between the cell outlet tubing and the pump-head tubing.
Controlling the Sipper from Software
Two sipper control modes:
1. Uncalibrated – Define pump time at all stages.
2. Calibrated – Define pump volume at each pumping stage.
Sipper parameters are stored in the experimental method. Sipper control is supported only in
Quant, Fixed and Scan modes.
See “Sipper Configuration” on page 63 for instructions on programming the sipper.
Sipper setup controls
Selection Definition
Sip Pump in the forward direction
(from the inlet tube towards the
flow cell and pump)
Settle Time to wait for the liquid to stop
moving before making a
measurement
Air gap Pump air in the forward direction
to push liquid through the system
Rinse Pump rinse solution in the forward
direction
Return Pump in the reverse direction (from
the pump and flow cell towards the
inlet tube)
20 GENEYS Family Spectrophotometers Thermo Scientific
Spectrophotometer Basics - GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160
Selection Definition
Measure Record the absorbance
Prompt Display an on-screen prompt to tell
the user what to place at inlet tube
for the next step in the sequence
Uncalibrated vs. calibrated sipper operation
Customers whose existing SOPs are based on a prior sipper system that did not support
calibration for volume pumped can continue to use time-based pumping in uncalibrated
methods.
In general, calibrating the system for volume delivered is the preferred method. Once
calibrated, the system will pump the specified volume at each step.
Fiber Optic Coupler and Probe (UV Vis systems only)
1
GENESYS Spectrophotometers
NOTICE Fiber optic cables can suffer damage when bent with too small a radius. A small
radius can also lead to light loss and reduction of performance even if it does not lead to
physical damage. The recommended minimum bend radius when working is 20cm.
Note The fiber optic probes that we supply are equipped with a shorter than normal
collar at the SMA connector. This helps to maximize the bend radius of the input fiber.
Probes purchased from other vendors will generally have a 25 mm long collar at the SMA
connector. This will make them difficult to install and may result in an unacceptably small
bend radius to the fiber as it exits the collar. If you wish to use a third-party probe, we
recommend that you specify a 13 mm collar.
Assembly
Assemble the fibers to the coupler as shown in the photograph.
1. Loosen the three thumb screws on the top cover and remove it from the coupler.
Thermo Scientific GENEYS Family Spectrophotometers 21
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GENESYS Spectrophotometers
Spectrophotometer Basics - GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160
2. Remove the protective plastic covers from the ends of the fibers.
Save these covers and re-attach them if you remove the probe.
3. Slide the tips of the SMA connector into the receivers and tighten the nuts hand tight.
Do not use tools to tighten the nuts further.
4. Loosen the hold-down thumb-screw, place the fibers under it, and tighten it down to hold the fibers in place.
Do not over-tighten. Be sure to route the cables as shown in the photograph above. This
routing pattern maximizes the bend radius.
Note The routing distance for the input and output fibers is slightly different so there
will be a small excess loop between the junction nut and the coupler. This is normal
and correct.
5. Re-attach the top cover to the coupler.
6. Remove the black rubber cover from the pick-up lens. Be careful not to touch the lens with your fingers.
7. Lower the coupler into the sample compartment with the white arrows aligned.
8. Ensure that the probe tip is tight. It screws on and off in the expected way.
22 GENEYS Family Spectrophotometers Thermo Scientific
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Configuration 1
GENESYS Spectrophotometers
Spectrophotometer Basics - GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160
Operation
The recommended operation wavelength range is from 220 nm to 1100 nm. Although the
fibers are solarization resistant they can suffer cumulative damage (they become opaque) when
used at very short wavelengths. Damage to the fibers due to solarization is not covered under
warranty.
Two configurations are recommended:
1. Place the probe in a clamp and bring the container with the solution up to it.
2. Hold the probe in your hand and place it into the container with the solution.
Measure the blank solution first and then measure the sample(s) – exactly as you would if
you were making measurements with cuvettes.
It is VERY important to avoid having an air bubble in the tip when making a
measurement. Air bubbles will result in completely incorrect measurement values. Tap the
probe with your finger to knock air bubbles out if using configuration 1. “Shake” the
probe back and forth quickly and/or tap it with your finger to knock air bubbles loose if
using configuration 2. Do not tap the probe on the side of the container. This could
damage the tip or the container.
Probe Tips with Different Pathlengths
While tips with other pathlengths can be purchased for the dip probe we do not recommend
their use with the GENESYS systems and have not tested other configurations. Longer
pathlengths typically lead to greater light-loss in the tip, reducing photometric range. Shorter
pathlengths are more prone to trapping air bubbles in the tip and it is more difficult to shake
them loose.
Performance
Fiber optic couplers and probes “cost” light in a spectrophotometer system. Having less light
intensity in the system means not being able to deliver the same photometric performance
with the fiber probe system that the instrument can deliver with cuvettes in a dark sample
compartment. Do not perform the performance verification tests when using the fiber optic
probe system.
Thermo Scientific GENEYS Family Spectrophotometers 23
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GENESYS Spectrophotometers
Spectrophotometer Basics - GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160
As a guide, in our tests, we found the system to very resistant to room lighting conditions and
to deliver good linearity to approx. 1.8A and an upper limit of measurement of 2.2A. If
performing quantitative analysis with this accessory and measuring above 1A we recommend
that users choose a quadratic (second order) fit for the curve and work in an operational range
below 1.8A.
Troubleshooting
Problem Cause Solution
Readings are unexpectedly high or low
with no consistency to the error
All readings are “off” by a uniform
amount
Change in photometric performance –
depressed ABS readings or maximum
ABS reading
Air bubbles in the tip during
sample measurement
Inspect the gap in the tip prior to
making a measurement. Take steps to
remove any bubbles.
Inconsistent small bend radius Use a large bend radius and keep it
consistent
Air bubble in the tip during blank
measurement
Inspect the gap in the tip prior to
making the blank measurement. Take
steps to remove any bubbles.
Bend radius too small Configure your experimental setup to
use a large and consistent bend radius
Damaged optical fiber Follow the recommendations above.
Record a blank and scan from
1100 nm to 220 nm with deionized
water. Save the spectrum. The
spectrum should be essentially flat
with < ±0.05A baseline noise. Large
spikes or discontinuities are common
signs of fiber damage.
Try using a different probe if you have
one available.
Contact Us
Probe does not work at all – no signal Cover over pick-up lens on coupler Remove the lens cover.
Broken fiber Replace the probe.
Tip loose, damaged or missing Remove and inspect the tip. Ensure
that the mirror is present and clean.
Attach the tip and re-test the system.
Coupler not inserted correctly Remove and re-insert the coupler
SMA connector not properly
secured
Remove and re-attach the SMA
connectors inside the fiber coupler.
Ensure that the nuts are not cross
threaded.
24 GENEYS Family Spectrophotometers Thermo Scientific
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