Thermo Scientific Nunc Cell Factory CF1, Nunc Cell Factory CF10, Nunc Cell Factory CF40, Nunc Cell Factory CF4 Instructions For Use Manual

Thermo Scientific Nunc Cell Factory

Instructions for use: CF1, CF4, CF10 and CF40

1

5

Unpack the Nunc™
Cell Factory and place
it in a laminar air
flow cabinet in order
to work in sterile
conditions.

Remove seal from
one of the white filter
adaptor caps.

Prepare cell
suspension in an
aspirator bottle, mount
with sterile connector,
and clamp.

2

43

6

Accessories:
Air filter, connector,
white filter adaptor
cap.

Immediately insert
pre-sterilized air filter
into the open white
filter adaptor cap.

Remove second white
filter adaptor cap from
the Cell Factory.

7

Insert connector.

8

Turn Cell Factory on its
side. Raise aspirator
bottle above Cell
Factory level.

Thermo Scientific Nunc Cell Factory

Instructions for use: CF1 CF4, CF10 and CF40

9 10

Gently agitate aspirator
bottle. Loosen clamp
and cell suspension
will flow into the Cell
Factory. Chambers will
initially fill unevenly
due to a difference in
pressure.

11

Turn the Cell Factory
90º, so that filling inlet
is up. Medium will
be separated with
equal amounts in each
chamber.

13

The surface of all the
trays will be covered
by medium. Remove
the connector, replace
the white filter adaptor
cap, but leave the filter
on and incubate.

12

14

When filling is
complete, allow
levels of liquid in
all chambers to
equalize through the
connecting channel.

Place the Cell Factory
in horizontal incubation
position.

To empty the Cell
Factory place it above
aspirator bottle, in the
position shown, and
the liquid will run out.

15 16

Tilt the Cell Factory to
drain it completely.

© 2010 The rmo Fisher S cientific In c. All Rights R eserved .
All trad emarks are t he proper ty of Ther mo Fisher Sci entific Inc. a nd its subsid iaries.

Asia: China Toll-free: 800-810-5118 or 400-650-5118; India: [phone], India Toll-free: 1 800 22 8374; Japan: [phone]
Other Asian countries: 65 68729717
Europe: Austria: [phone]; Belgium: [phone]; Denmark: [phone]; France: [phone]; Germany: [phone]
6940, Germany Toll-free: 08001-536 376; Italy: [phone] or 434-254-375;
Netherlands: [phone]; Nordic/Baltic countries: [phone]; Russia/CIS: [phone];
Spain/Portugal: [phone]; Switzerland: [phone]; UK/Ireland: [phone]

North America: USA/Canada [phone]; USA Toll-free: [phone]
South America: USA sales support: [phone]
Countries not listed: [phone] or [phone]

MNLSP CF10INST R 0210

To decontaminate the
Cell Factory after use
it is recommended to
autoclave: 60 min,
132°C.

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